THE VALUE Q? VARIABLES OBTAINED QURING A. SU’EuMAXIMAL MULTIRATE AND MULTILEVEL STEP TEST IN PRIEDICTING MAXIMUM QX‘I’GER CO-NSUM’PTION ON THE HEAD-MILL BUN Thesls {0' “no Degree 0} M. A. MICHIGAN STATE UNIVERSITY Brian W. Eisner 1984 THESIS LIBRARY Michigan State University ABSTRACT THE VALUE OF VARIABLES OBTAINED DURING A SUB-MAXIMAL MULTIRATE AND MULTILEVEL STEP TEST IN PREDICTING MAXIMUM OXYGEN CONSUMPTION ON THE TREADMILL RUN by Brian W. Eisner The purpose of this study was to determine the rela- tive value of variables obtained during a sub-maximal multi- rate and multilevel step test in predicting maximum oxygen consumption, per kilogram of body weight, on the treadmill. Thirty college men were used as subjects in this study. The multilevel step test consisted of five steps ranging in height from ten to fifty centimeters. Each of the subjects stepped for three minutes at each step at a rate of thirty steps per minute. The multirate step test consisted of a single step of a height of 37.5 centimeters. 'The rates of stepping used here were 12, 18, 2A, 30, and 36 steps per minute. Two minute recovery measures were recorded at each rate of stepping. The treadmill run consisted of running 7mph on the flat for the first minute. Each succeeding minute the grade was increased 1% until termination of the run. BRIAN W. EISNER The results indicate that maximum oxygen consumption, as measured by the treadmill run, could not be predicted with a reasonable degree of accuracy from the heart rate and oxygen consumption variables of the multilevel and multirate step tests in this study. In fifty per cent of the subjects the multilevel step test was a maximal test of physical capacity. Those subjects who finished the multilevel step test, had consistently lower heart rates throughout the test, than those subjects who did not finish the test. THE VALUE OF VARIABLES OBTAINED DURING A SUB-MAXIMAL MULTIRATE AND MULTILEVEL STEP TEST IN PREDICTING MAXIMUM OXYGEN CONSUMPTION ON THE TREADMILL RUN By Brian W. Eisner A THESIS Submitted to Michigan State University in partial fulfillment of the requirements for the degree of MASTER OF ART Department of Health, Physical Education and Recreation 1964 ACKNOWLEDGMENT The author wishes to acknowledge Wayne VanHuss for his warm friendship and guidance throughout this entire thesis. TABLE OF CONTENTS CHAPTER PAGE I. THE PROBLEM . . . . . . . . . . . 1 Introduction . . . . . . . . . . 1 Statement of the Problem . 1 Significance of the Problem . 2 Limitations of the Study . . . . . 2 Definition of Terms . 2 II. REVIEW OF THE LITERATURE . A Discussion of Maximum Oxygen Consumption. 4 Selected Methods of Determining Maximum Oxygen Consumption on the Treadmill. . 5 Maximum Oxygen Consumption in Terms of Kilograms of Body Weight 6 Exercise Heart Rate 6 III. EXPERIMENTAL METHOD 8 Subjects 8 Testing Procedure and Equipment . . . 8 Test Periods . . . . . . . . . . 11 Overview of the Statistical Technique. . ll Prediction of Maximum Oxygen Consumption From a Sub—Maximal Step Test . . . . 12 IV. RESULTS. . . . . . . . . . . . . 13 Discussion. . . . . . . . . . . 24 CHAPTER V. SUMMARY, CONCLUSIONS, AND RECOMMENDATIONS . Summary Conclusions Recommendations. BIBLIOGRAPHY . . . APPENDICES iv PAGE 34 34 34 36 MO LIST OF TABLES TABLE I. Correlation Matrix: Selected Multilevel Step Test Exercise Pulse Rate Variable II. Correlation Matrix: Selected Multilevel Step Test Oxygen Intake Variable III. Correlation Matrix: Selected Multirate Step Test Exercise Pulse Rate Variable IV. Correlation Matrix: Selected Multirate Step Test Exercise Oxygen Intake Variable V. Correlation Matrix: Selected Multirate Step Test Recovery Pulse Rate Variable VI. Correlation Matrix: Selected Multirate Step Test Recovery Oxygen Consumption Variable VII. Correlation Matrix: Selected Multirate Step Test Exercise and Recovery Pulse Rate PAGE 15 l6 l8 19 21 22 23 LIST OF FIGURES FIGURE A PAGE I. Heart Rates of Those Subjects Who Did and Did Not Complete the Multilevel Step Test . . 25 II. Oxygen Consumption Measures of Those Subjects Who Did and Did Not Complete the Multilevel Step Test . . . . . . . . . . . 27 III. Mean Heart Rates During Exercise and at Two Minutes Recovery on the Multirate Step Test . . . . . . . . . . . . . 29 IV. Mean Heart Rates During Exercise and at Two Minutes Recovery on the Multirate Step Test . . . . . . . . . . . . . 3O CHAPTER I THE PROBLEM Introduction Physical educators have long realized the need for an effective test to measure the maximum physical capacity of an individual. At the present time two tests, the treadmill run and the bicycle ergometer, seem to be the most widely accepted. Unfortunately both of these methods of testing are confined to laboratory conditions. At this time, the two most accurate variables in predicting maximum physical capacity seem to be maximum heart rate and maximum oxygen consumption. Testing has revealed that the treadmill can obtain these maximal figures. What has not been satisfactorily done however, is to relate a treadmill test'to a sub~maximal step test to determine if the step test can predict both maximum heart rate and oxygen consumption with a fair degree of accuracy. Statement of the Problem The purpose of this study was to determine the relative value of variables obtained during a sub-maximal multirate and a multilevel step test in predicting maximum oxygen consumption, per kilogram of bOdy weight, on the treadmill. Significance of the Problem Maximum oxygen consumption has been termed as one of the best physiological predictors of a persons circulo— respiratory fitness level. The only way we have to measure maximum oxygen consumption, at this time, is through one of the high intensity laboratory tests. By having to measure maximum oxygen consumption through high intensity work a number of uncontrolled variables, such as motivation and loss of precision in measurement are inherent in the experimentation. It is clear that if adequate prediction equations could be derived from sub-maximal tests that application could be made under less sophisticated conditions resulting in a useful, practical test. Limitations of the Study l. The motivation of the subjects could not be directly controlled. 2. The amount of rest and food the subjects had before being tested could not be controlled. 3. Diurnal variations were present as in most repeat test data. A. The sample was limited to college age men. Definition of Terms Multilevel step test.——This is a graduated step test with five different steps with heights of lO, 20, 30, 40, and 50 centimeters. The subject stepped on each step for three minutes, at a rate of thirty steps per minute. Multirate step test.-—This step test consisted of a single step at a height of 37.5 centimeters. The subject began stepping at a rate of 12 steps per minute for one minute, after which he received a five minute recovery period. The subject then stepped at a rate of 18 steps per minute. This procedure continued for 24, 30, and 36 steps per minute. Maximal treadmill run.-—This run began at seven miles per hour zero per cent grade, with no warm up. Each succeeding minute the grade was increased one . per cent, with the Speed held constant. The test was terminated when the subject was approaching maximal voluntary effort. Maximum physical capacity.-—The maximum oxygen ~consumption figure the subject reached during the treadmill run. This figure was recorded in terms of oxygen consumption in milliliters, per kilogram of body weight (O2 //kg). max CHAPTER II REVIEW OF THE LITERATURE The review is limited to the pertinent research related to oxygen consumption and heart rate on step tests and treadmill runs. Discussion of Maximum Oxygen Consumption According to Hill (12) maximum oxygen consumption is reached when oxygen cOnsumption per unit time has attained, ". . .its maximum and remains constant Owing to the limitation of the circulatory and respiratory systems." Robinson (17) states that the, ”Maximum oxygen cOnsumption of a man represents his maximal rate of aerobic energy release for work, and it is attained in the second or third minute of any exhausting work in which the energy requirement Of the work exceeds his maximum capacity for aerobic metabolism.” The term maximum oxygen consumption must, itself, be viewed skeptically. It seems clear from the work of Christensen and Hogberg (9) and Taylor, Buskirk and Henschel (20) that the actual value reached for a given individual depends on the nature of the physical activity. It is therefore, maximal relative to a given set of condi- tions, which must be carefully defined, rather than in an absolute sense. Selected Methods of Determining Maximum Oxygen Consumption on the Treadmill Various methods have been employed on the treadmill to obtain maximum consumption figures. Taylor, Buskirk, and Henschel (20) report that using a constant Speed (7 mph.) and increasing the grade in steps of 2 1/2% each day, is more satisfactory then using a constant grade and increasing the Speed. Expired air was routinely collected between one minute forty-five seconds and two minutes and forty-five seconds of a three minute run. It was shown that the oxygen consumption at this time had reached an apparent steady state by examining the oxygen consumption for the following minute or running. The increase in oxygen consumption, associated with an increase of 2 1/2% grade (below the maximal oxygen consumption) is approxi- mately three hundred milliliters per minute. If the oxygen consumption at two different grade differs by less than one hundred and fifty milliliters per minute or two and one-tenth milliliters per kilogram of body weight per minute, it can safely be assumed that a maximal oxygen consumption has been attained. Mitchell, Sproule, and Chapman (15) used basically the same method as Taylor, §t_al,, except for two modifi- cations. The first modification was that the subjects were required to grip a supporting shelf with their left hand while they ran. The second modification was that more than one work load was completed during a day by each subject. The subjects in this study were not actively in training nor did they appear to be excessively fat or lean indiVid— uals. Maximum Oxygen Consumption in Terms of Kilograms offiBody Weight Investigators have found that oxygen consumption in terms of kilograms of body weight gives a more meaningful figure in comparing subjects of different weights. Buskirk and Taylor (6) report that oxygen consump— tion per kilogram of fat free body weight seem to be a more exact measurement of a persons maximum oxygen consumption. Exercise Heart Rate Robinson (17) points out that an average man under severe work has a maximal heart rate of approximately one hundred and ninety beats per minute. In 1921 Hambly and Hunt experimented with pulse rate in regards to walking up and down stairs. Later this method was given up in favor of the step up exercise in which a thirteen inch step was used at cadences varying from six to thirty—six steps per minute. Hambly, Pembrey and Warner (10) used the thirteen inch step with a cadence of eighteen and twenty—four steps per minute in order to compare various tests for assessing physical fitness. They concluded that, "For many reasons the pulse affords the best test of efficiency." Campbell (7) used eighty medical students as subjects. He employed the technique of Hambly using a thirteen inch step and a cadence of twenty-eight steps per minute. Campbell concluded that his step test showed a greater difference between the unfit and the average men, than between the average men and the athletes. CHAPTER III EXPERIMENTAL METHOD The purpose of this experiment was to determine if maximum oxygen consumption on the treadmill could be predicted from variables obtained during sub-maximal multirate and multilevel step tests. Subjects Thirty college men between the ages of nineteen and thirty were used as subjects in this experiment. Each of the subjects had no previous record of heart disease. The subjects were divided by random selection into three groups. Each group then began with a different test. The subjects were all tested during the day between eight a.m. and four p.m. The temperature in the testing chamber ranged from seventy to seventy—eight degrees Fahrenheit. The average (wet bulb) humidity ranged between fifty and seventy per cent. In only two cases did the humidity rise above this maximum figure. Testing Procedure and Equipment The first step after the subject arrived in the laboratory was the placement of the electrodes. The electrodes used in this study were the eight milli- meter Grass Electrodes.l The procedure in the placement of these electrodes was to first apply "Tuff Skin"2 to the area approximately one inch directly above and one inch directly below the left nipple, and also to the lower midline portion of the back. The "Tuff Skin" was used to increase the adhesion of the tape, which holds the electrodes in place, and to retard sweating in that area. A small area of "Tuff Skin" was then cleared away with rubbing alcohol and roughened, in the three above mentioned areas. The electrodes were then filled with electrode paste, and secured with one-half inch width strips of adhesive tape. Over these strips are placed one inch squares of "Elastoplast" tape.3 This additional tape is to help give added firmness to the electrode placement. Longer two inch width strips of adhesive tape were secured over the "Elastoplast." Finally, the three electrode wires were taped together, with slack, on the left shoulder. After the subject had the electrodes in place they- were plugged into a Sanborn Twin Viso RecorderfAequipped lGrass Medical Instruments, Quincy, Mass. 2Banner Manufacturing Company, Nashville, Tenn. 3Duke Laboratories, South Norwalk, Conn. 4 Sanborn Company, Cambridge, Mass. 10 for electrocardiography. This yielded continuous pulse rate readings during the exercise. A Sanborn Electro- cardiotachometer was used to give a visual reading of the heart rate during exercise. The tachometer output was recorded using a Sargent SR Recorder.5 During exercise the collection of gas began with the subject breathing into a Collins Triple J Respiratory 6 Valve. The expired air was then taken by way of twenty inches of one and one—quarter inch British Corrugated Tubing to a Hans Rudolph Five Way Valve. To four of these valves fifty liter Douglas Bags were attached. After the air was collected in these bags, they were care— fully removed and readied for the oxygen, carbon dioxide and volume analysis. During the gas analysis the gas was forced through 'the Beckman Carbon Dioxide (LB-15A) and Oxygen Analyzers (E-2fl'at a rate of two—hundred milliliters per minute. These instruments measured the percentages of carbon dioxide and oxygen in the expired air. The bags were then removed and the volume of expired gas was determined by pumping the gas through a Franz-Muller Calorimeter.8 5Sargent Company, Chicago 30, Ill. 6Warren E. Collins Incorporated, Boston 15, Mass. 7Beckman Instruments Incorporated, Fullerton, Calif. 8 Max Plank Institute, Dortmund, Germany. 11 Test Periods The experiment was conducted during.the months of May, June, and July. The subjects were scheduled for their tests at the same time each day. However, due to conflicting schedules of the subjects and laboratory technicians there were exceptions. Overview of the Statistical Technique The purpose of this study was to determine the relative value of variables obtained during a sub—maximal multirate and a multilevel step test in predicting maximum oxygen consumption per kilogram of body weight, on the treadmill. To achieve the purpose of this study a statis— tical technique was needed that would be able to predict a certain criteria from another set of criteria.“ The statistical technique used for analysis was multiple regression. With this statistical technique a dependent variable is predicted from a number of independent variables. The dependent variable in this study was maximum oxygen consumption per kilogram of body weight. The independent variables_are heart rate, respiratory quotient, and oxygen consumption on the multilevel and multirate step tests. The multilevel step test is a graduated step test with five different steps at heights of IO, 20, 30, MO, and 50 centimeters. The subject stepped on each step for three minutes, at a rate of thirty steps per minute. 12 The multirate step test consisted of a single step at a height of 37.5 centimeters. The subject began stepping at a rate of 12 steps per minute for one minute, after which he received a five minute recovery period. The subject then stepped at a rate of 18 steps per minute. This procedure continued for 24, 30, and 36 steps per minute. Prediction of Maximum Oxygen Consumption From a Sub-maximal Step Test Astrand and Ryhming (2) found that a step test with A a sub-maximal work level would give good information about the subject's aerobic capacity. The best results were obtained when the test work was of such a severity that the heart rate during steady state attained a level somewhere between 125 and 170 beats per minute. Martiz, §t_al, (14) found that the maximum oxygen intake of an individual can be estimated with a coeffi- cient of variation of 6% from population means relating the oxygen intake for various rates of stepping to rate of work. This value is of the same order of magnitude as the residual variance of oxygen intake due to day to day ‘variation and observer errors. CHAPTER IV- RESULTS This experiment consisted of three tests on thirty subjects. (Two of the three tests were the sub—maximal step tests, while the third was the maximal oxygen intake test on the treadmill. The purpose of this study was to determine the predictive value of the sub-maximal variables in predicting maximal oxygen intake, per kilogram of body weight, on the treadmill run. The variables selected for the following tables, and regression equations, were chosen because of their high correlations with the criterion measure, and low correlation with the other selected independent variables. The initial selections were made from the computer data and multiple regression analysis using more variables. The selection was made to yield the highest multiple regression coefficients possible from the data. The correlation matrices are presented in Appendix B. Table I presents the best three pulse rate variables from the multilevel step test. Also shown in Table I is the correlation between each variable and maximum oxygen consumption, the betas squared, and the per cent contribution to the total predictive variance. 14 In Table I it should be noted that one of the vari- ables, pulse rate during the ninth minute of exercise, is not contributing to the multiple regression coefficient. The third variable used in Table I is a pulse rate differ— ence, which was merely created by subtracting the pulse rate of the sixth minute from the pulse rate of the eleventh minute. Pulse rate during the seventh minute of exercise is contributing the majority of the prediction of these set of variables. However, the multiple regression coefficient is relatively low for these data, indicating that these three heart rate variables would not be sufficiently accurate in predicting maximum oxygen consump- tion as measured by the treadmill run. Table II presents the best three oxygen consump-' tion variables from the multilevel step test. Each of these oxygen consumption variables is in terms of kilo— grams of body weight. In Table II oxygen intake per kilogram of body weight during the eleventh minute of exercise contributes over ninety per cent of the predicted variance. 15 mac. n m who.b use. n m . m . m . H . I Hmo + xmooo + xmooooo I xmooo I I w oo.OOH HERA. .dee HHIo ooCoEOMMHQ mm.oH omo. mmfi. 0mm. Ram. opmm omfism IIIII Hooo. wmm. mam. .EHE Bum opmm omasm .eHs BBS mo.mw sea. ma:.- oemm omasm soap mm .dfls HHIm .cfls .dHe HHHEURer moaomflhm> Isoahpcoo oodohommao Bum cps .pz zoom oo ammo mom oumm omasm opmm oumm .mx\oxmqu omasm omasm mo ESEmez mmqmmHMd>.MBMQHBQD2 QMBomqmm H mqm Iseesedoo .oz seom .p: zoom .pz soom .hz Aeom oo pcoo pom mo .mm\ mo .wx\ mo .mM\ .wM\oxmp:H OEMpCH mo oxmucH mo oxmpCH 0 mo Eseflxmz mmqm¢Hm<>.MMMAHBADE Qmaomqmm ”XHmB peoo pom maoum OMIwH mgopm mm .p3 zoom mo oocohommam opmm .wM\oxmucH oumm omasm omasm mo Eseflxmz mesmeHm<> meem mmqpm mmHommxm Emma mMEm ME _Isnfigpqoo m hampm :N mmoum ma mQOpm NH HHHEUMOEB de0 Mom .pz hoom .93 zoom .93 hoom .pz hoom Mo mo .mM\ mo .m&\ mo Imx\ .wM\oxmch oxmpsH mo UXMpCH mo oxmch NO NO Esefixmz mmqm mEeBZH zmewxo mmHommxm Emma mmem meamHequ mmeomqmm .meeaz 20HeH mqm Isnampcoo mmopm OMIwH woman mm mmoum NH .p3 zoom o0 pcoo pom monopommam oumm oumm .MM\oxmp:H oumm omasm omasm omasm mo EBEmeE mmqm-meoomm.BmMB mmem WB.mqm<8 22 HHO. I m use In 0H3. u m smo. + mReHs. I mamme. + Hsmo.H I » oo.ooH mmm. mHmooe OCHE\ woman mm .ez zoom mo .wm\ mm.mm HmH. emo. com. moH.I ossoeH mo .GHE\ maoum 2N .o3.soom Mo .wm\ ms.eH omo. Hes. mmH. oxopeH No .GHE\ maopm NH .uz seem so .EM\ om.Hm soH. emu. oasedH 0 goes mm .est .:Hs\ .:HE\ HHHeesohe moHolos> IBQHmpqoo woman mm woman :m macaw NH .vs zoom.wo peoo dos .uz seem .o: soon .u: seem .wu\osooeH no .mux no .mux so .wmx we assess: oxsoeH m5 osooeH mo osoeeH mo E II‘ wmgdem¢> ZOHBmEszoo ZMQNNO Nmm>oomm "NHMB MHmooom :w.os mow.H mow. pom. mom. Smm.I opmm mmHsm .cHs\ mmopm mm mpo>ooom mm.>m :wo. oww. mwm. :mm.I whom omHsm .EHE\ moose omImH oocopommHQ :o. Hoo. mso. qu.I opmm omHzm .CHE\ mmoum om mm.H mzo. Hm:.I opmm omHsm coHp m .GHE\ .CHE\ .QHE\ .CHE\ HHHEomohB moHQmHgm> Iangucoo m mmopm OMIwH mmopm mmopm maopm mm .pz zoom Ho pcoo pom monopommHQ mm omImH opwm .MM\oxmch who>ovom .oom .mmHQ omHsm mo EBEmez opmm odem opmm spam omHsm omHsm WWE¢m mmHDm mmm>oomm Q24 mmHommxm Emme mMBm WB mqm- 9315-5— 23» tug- ! 9.0:: "hr afigah 8 =3 swim zo-.—-(I—Dm(-—- sang: 624 33:: 3138 . 10:133. 02¢ 1033-9: 0.: 19:02: .22»)... .51... I {9:932 433:: .151: 3 55:13 5.9921: :4: I! 55:31; :«S 2122!: - , -- - -- -..I. - I. o u _ IIo n m m I NF mflqqlflqn n. In. aniwuw. 8N... if «or. n IMMEI N I nmm. fin. =or CE N1 3.0 wwu. flu. E..- 60-- 31.1.3»... I “Md. kahuna“ I E. Cu... Ed... n3. 5%. «he. owqrdqdi dqxr I NSF vqu «NM. :w. xvn. _wu- «mu. IILW ._ I “.2: N... t 51.1.- MME N3- m2. I I 34.... «3. =2. «Er «3+ 4.3. Ntr NS. mam. ma. NE. «E. I a..- L I find. the 3.4.1 «3f 3.- «N11 quf «RV uan 0%- 83. wqu .36... «66.. EN. quvI Mum. Sm... 5v- New- -.. I. III NNQ- “.qu “MN. ram... an-I \om. “on. Hxxf :0- QQN. d\\- Nun... W10- E4 “\Yfl. “Sf thf Mudf mdxf .........--... I War «m1. gar .I :n. qu. 3.x... :qr ME. «mi \tf «E. «3. «fix. NS. 13. NUT- $4- -WIMAI . I an. M3. J3... . :6. furl +2.- :1- _.mdf on..- Bar «.3..- kin. in- ASH. _ I 02. and. N I \m\. uw‘ 22f hudf v.3-I inf 5dr «imf in. won- 3.“... d... m4.-r-.z.....MW._ I +3. wfif 3!. MN. «Er rh~r war Snr «if My»..- Tr. Nu . .43- 9.4. N... -I I wear EN. ME :4. «Sr :1 wNov ntr 2m... M3. . .1 2hr 2a-- ...I..--I... n. ,_ I ha. o+~. 3m. mum. EN gm. hnn. Vnm- in- GI: NW... .25-. .II. I i. _ I MNM- Eh. ham- d~n. T4»..- in ohm. ..an .43.. 3... wk... Iu-4 ... _ I 32.- «am? «Ne. 2+- in. IE“. JMEI EMF 17.- ..I.. «I .. - m I NW“: +3.. “.3. v3. .3“. . SII “an.-- :Tr {TI Nu. _.I I rm»: tum. ..I.. «mm: :mrIjfiII v1.5- Jwfi Id.-- .II_ I m3. ml. 3%. Err to. ,- rum..- MEII 43-1 I .I..I_ I So. .. qflf S... I t... - 4:.--» u... ....I1 I Ch... «Mei .42...- «an- WTIW MG. - . . I ...nhfl ,uw - Mn“..- .3... ._ .54- ....- .I. -xIquJ... «Nu: 1N4 ISM. II in..- “.4; :5. I.I.I I a... . HEW . III 3.3» .3. a .2... . ..z n .5. I 2. -... .. _.z . . . 3., 3..., .2... ... 3. .. 3 .- H . - It“?! {flat-Pr ilhfirTIOuH—M» Jilouu' Iliad“; first?” 3 :aHu—rnrjdxcUHU—NL 41!”. up”! )UHWM. {Hun :ruh cum” )CUWU :5”on ””4”...” urn...“ .. II P ..I -.. 1......» .0 a Ga .Gd «:5 “0 (...II. «Juno 3...: 3. min t. 3 2. 51%.» av}... J...hd ~23 II} by? .03....“ 3S6 ...a.h......n a. a ... a u .. I a toga-(h uZo» g g 3.0:: I... 10339: g .20.:32 3...»... .51... B .6551: :45 3 var—(43.4.. 8 3.3 hmwim 2035393.. xu-(uwuu ot< 3.0:: ewt..:>.z: 56 toads... 3;»... .254... 8 20. r (=0: 3 Civil: ubh_w-u>_13 u>dra 13.20.} I th- «mu. mac- filgfldfll. EM. «we. gals-H.113“... «5... MS. 3.. w - 2:. a... «#1 war 9a.- 43. NE. 3d- 1..u.....-.... I hum. who... .8... HQ. 49- to... :3. @3113? Sq- “do... a . Mac. 2.0. :d- «Md- mun. i. 53. vi. 45... «E. - ...H.... .I exordnmf :4. mg. 5.9. .. m!” PS- a .. «ha- muqf 4.3. Nnof i- «4N- arm. «a... rd? var run. . HM... I ... hm“- tn... mafiai a. mum- «flu. ddq. mdq. v.5. an- at- :2. .30. $5.: ENC .. ...ww ..F I Nd” w: n: 103.! N3... Nut... mimil 5*.I h.~+.l '94.] «RN I 3.1 ..qu- L30. «ix- «WC. 30... «2.- 1.3.1 at. u... a ll Nno. Eu. .31. .I vaf a. mi... mazl Nah... um‘f MG: mNV. M2- v.3- «xx. wow. :3. I... QNm. WIN- an... +3": WNW 3+... N3... 3n... «Mn... «2... MAM”. +2- a1 :1 mmw- 3N- I mkqfl haw... “N“... 84.13.“... .33.... Now... mmfl. huh. van. and. $6.: .30.! $6... $8. . I “xx. 35. .nflu. N1“. :3 04‘ Mai. ME... «2. «E. :3. Mam- mMo. . I own. MM“- «MM. :+- NJN. «4*- 3+. 3*. rail 50... MQU in..- .20.- \N\. N2- .r......... I... dam. ww~- 3‘. «3. 5d. ER- mum- 30.1 Too. «M1 0:. rug- r:. m)... . .21.. 1.-- I «a. «a... Eh. NC. an. oi- 9m... 37! 43... n4- .4“... Maw. .33... . ImJ. II 3...: Nxb- in Na. - “nu. Mum..- N3... Mwu... $3. .9... 3.x. «we. . .u. I n: NE. 34. .34. 3:. «3. Eq. Ms. “1.... «$2..-. 1.- . I Mun. N3. row. .03... owe. 36.1 m...:...... 3r..- rlf v.9. . .-1 . I ~Qm. hwm. 55... Na. Ex. 30.x. :1... Tad.-. SINCQWWI I wam- Tux... ..me... NSF S- :9. $3. 4.1.. I... .1. II. Em... «3... who... .36- 3...... 3.0... 45.- r .4. ...... -. «hm- NE. T3 .23.- in..- 2...- i .. .1.. I a..- . m3- n1--- «1...- f-.. I. .1... a..- ...I.- :1- 2...... .1 a... L...\ . ..v. . mg“- \\t ”.11 1.!111! . u w- I)... twp: 1.. .n. .. “..M r.» TN Ln: {3. rngn fig T\nv~ If. .333 rm...» 3...qu Fifi... I...“ m. 1.. H.. w..- .n z... ..m. 1...... .- .. . .3. I I - wily.» mum-MI Mun...» W->Ln»w.»b>rr 74>»)? .102)? ...Inb BIJL Yrs? .rrrnF Hf“..— .mdfau . pt I m...» .C. wit.» flip...» a I. s .. 0 MM..-» .. K. ..I.; . . .21: . . 351$»: 13...? m u an ad U... ... a 3.4. «who .33.. 9.5.1 man.“ 1-..... ....,.,.~n.......C Saw ..I...- ...r. I. .-.bJI ..I.. 1 ..L. £8252» 2.9 (.F .013. I: 3.3». P. 20:25.3. 8 up: SWIM ZO-PSDD(.P .623. 92 3.3: 3.533 £02392 iuawnnuuuwwkudo 5.12:3 .I‘|r. 'lu‘ '. . 5".I ! a a a 8 n a R a n a a a a ..I. 3‘... EM. .3. bed ._ ANSI one. .37 .1 at... dad- duo. ado. a.m.: “2.: M3. afiflmfiaa I. «E. a muu.Jun{[.id.1 I ho. .. 2...... N .. 2.... En. dud- n. 12.... «5. 3e. 3.? 2.11 .n. 3 n...» 2 I H44. EN EN. N2... M3. N5 «JG Nux d1? flu: n! v.2. 3N. MM} «.3. 9:2 inn.“ AM... i... whnilund. "hr... :7: t2. *2 dud. +2. 1!..- to... daN dam. «Na. 5... 5?: $5 En: II ham. r 3+. E. in... numf .n 2a. 2a... 3.....- air air 95.. E... 3.. ........n-u..w.w:2 III +OMAI ”NH" ”‘2 Nntl. NHQ.‘ Tmf N31 ““0. g. MSG. ~01 OJ—. Ord.‘ «IqllhufiNhHHade— ll Mdm. five. and... mm..- «at mvN one «I... «3-- N2... Q- M3. C1: 2-33%.: I 03. v3. 51 3.1 «3. an. «3.. E..- 5v 9.... v22 «34 Sawflfimwfl III 34 Nun Qfld. his 6mm. mu. «um. Wu.“ Gm.“ er. Ni- 3.34:1. Amman. Il er. 3h Em: «Md. «6. man. 2..“ «id. flvd M:. ..x......:.« ”um: I, Sn. oi- :4- n5 :2. «MN- Maw. E- n3.-.1........4.n.\...2 III. a mint. g4- “Nd- “Nu. wfifi‘ 43. Haw—AH.” WWW; ..ll 2:. «Eu. In- muq- 5v. fifi. n6. M....x..z....m..‘n. rnn- g 31. may- :1. “Nu. Wmfiflnwm W: \l 5.. qt. ...vw «C- Fa. ..H........w....u.....flw. \II: 4.; :~ 57 $0. www.cw...m m.“ I‘llll Mllfl.\1 UH.“- WJOqI 11. J13 41d! E- .2: given?“ :1 3.. ...m.¢...m...... .||I ..1. 4.31 it“: afid (.31.... viruJ ..I“;M fligjlflqflm ”DIN .‘ iQN 4H. niN JG 31d viii-IN 3n. 2.!. .uua 1:0 .3... :iNLéL swan 4.3.3»... 1. dc ‘ll Alida-e :43... .11"? 5‘.» I}: I}: ...th I}? I‘M. 303 .530! kW!“ ’3'! t} N~ $5041 Sknn Iw¢V (3 .ra ..Zou Vuzusuub 333:...— aootfi waft!» DAL...» nip)» firtfi fit)» his» 3:2. “SEC hut)» Natar mi?» N4"; I53. «305:1. niw 516.... ..o .11 :1... 1. 3.3 1. :ca .3 91:0 3“.an 3.}0 345 aiqu fin P. 9:an «:6 an «EUR. 1.32.. no :3 p: .7.” .1 Bag 5. £9452.— 9.8.— 80-5- 2 333-.» :33 63a. .5 use. .I..! Emlm ZO..—.<._Dm<._. 82.15.45. 8 :3 10:33: 9.2 .29333 33:: 1:3: .0 5.3.4.! 5.2.5 :1.» I! 3332:: 21m 146:0... F: u u . . .t I I. . .-y..|.o -5: I. I.... I In--. 58 . n '1‘. a a a _n 3 Q Ca - . ha .1 nuns. 51%| 3?. «m1: NHC. one. «a.m...nlfiin m.!. 5H. man. Htfnnn... 9*. r18... #5... Mn..- «3... «41 3:. 3m... oz..- 3. .. ...- in 1 RV1. Na? *3. «RN Mi. Mm NNN. ..«xq. qu. $00.. Ndo. .GQM: a. NE... NW? «a... .34. Max. Gd. wt. «mm: Mun. ham- awn/H.431... 1 .3? $1.. «5.. a. .3..- 2.x}. 2.»..- m3 *2... «*1. +2... m5... :3... $1 «I... .05.- am..- .3“ wa- «0+... wflf $1... 9...! :Jdizna 1 nfiq- 3N. m$\.\ NS... QM. Ni. a.m.... v.3... 3dr C gnu M3. SN-.. Maor $1.. .1 «Hr .. g... wvm Mvfi 3?. .13.. 2hrd1... fin 1 .th.NNu. dmw. 2+. «on rt“. at. *w mi EN- omm1 Ni. oNN nu“- hgm. in- mum. v3. +0.0. 3.1.- an.....u..,...:~a 1 .93. moqm. E. ~.qm-+.hq.n «.13.. «GM mun..hm.N- omw. SM... Mun. EV ma... :1. i4. NAN w -. $4.- .3 - rwwwflvwz II 4:. 2M. :1 a3-.. tr. 311$“ 1%. MN? 3.... mm to. New. M3. .03. v.2. KT. $4. FE- uqa.~.uw.w.a - - a.m.... qhq. rnq... Mom. dud. a.m.... m9... woof 3n. Rd. W : .03.. Man. and. 5:... .1». ..1. .4- 3......WAd2 - - $5.. In..." Hod :1 «no. t... m3. 0%... mow 1.1x me wmw P1. SQ. N. :1 a... . 10%;“: - . ~14. NMo.M~Q.l emu- wm Bum: www. “PM «5.0. Mm... «MN. own. u. $1 1...... ~11 a.m.." ...u: . - “Q. 05.- «.3. NW... EN- \mN. m3 T47 m3. :1. .Jmm. m... u... . am... T... 3.... warm“: - 0mm :u. 39... :3. Law. +mw .21 3,-.- 314;- «a... .w. 2.- .11.. 1W1: 1 mmm. 9v..- 3... mum! mum. Fm Eu... 3...... $4.. «31 “.3. :1: J . 2.1.0.: dqu 23... 3.. in. ham. an. gm. .3..- 94.- 1M1- M- \n\.-....-.....1..fi-2 - “no.-- ...il N3... NV... MM... “1.11 3:... Min..- 2&1 ...I- . .1411. 2 v2.1 3?. $1. :6..- cl... .1. fiu- 3.... 1. v . 1:...1. __ 1 H4... 2.» in «mu. “1... 3.“- .1. 1.3-7.. ..... 2 hum. 2». +4 1.... 1.....- FE. .... 1.- . a 03.. 2?. PM- 1.-- w... . . 1.1. .. . .v.-..u. $.11 Wu“.-. .....-.- v.1. m.u . .fi ...- 3... T1,-.- 1. T . .. ...1U1. v.1 ”.4 - 2..“ .uu- 41 «.1 4.. r-r. 4.1.“- ...., . n - 49w .... 1 ”n - 3......1 wuwhfl __I¢ on- v. T». a“ tfiadfllrfilhn “Jam“ “mm“.m. (Nu My Tm FF»: r}. ...3 ...qu r\non5_1.¢:i.x.fin_.?_16r 2.1.fiv 2.7.3“ ..I.»? 7.1.902: Cr .1...» «:13: - 71.. its: 5.. 5F... ah; F8 ..CJbLaF Niovw» Wkr I u)... L . L \lfisrlr la 13L ...E:L .541 V3.3» Que .Uua at am ..Q uld .25 “11k, at...» .dt?» WEE...» ..I..»SL. _anI .53! C13; .53... 2.1: .53.. :33: gr : .52. 5.1 gt}. 3.0 a...» rib 51.6 426 15.0 166.33.: 3...: It». +2 9. 0 L330 L330 3 t. 3.61. 3:40 3.70 3:...” .330 LS. - ex}. 3...... r... 2 :3. -1 ...a 5 3833: 2.8 1 3‘1 >5; gin. 8 aka Buzm 20.2.59: rt .1 II! 0.0... . 10:33: 92 10:53. 33:: £53.. 8 past-«.8 E322: .2: 21:5... -. 10 .-...1.. I; .2 3.03..» :33 ROSE-it USE ONLY. R ”"111 ifixififlujlflfijgfiu fififlflffl‘fi N 315% mu“