... {4...}..- c... finknfiwfim O. §Id~|591 ...-III ..Y iv :ch ... ‘\‘Nr‘t“\. 1‘ 0%mflflpuflwlqu . ‘ ‘V \\. O . . . o a .. . ~ to Q. . o. v u. . . . 1 c 1 .' I n. . .. I? o. d v . l -\O. c. ‘ -. firming. 2.. ...-......--..«.... . awn.-.- . . C . A . . - . K. - . 4] 9:“ 4‘0';9:0!'\.flv iv? ‘Hu-WIOQI‘M‘ .Hfi‘. 1”. o ulu..v-cv-- 13.... ...-C .fl~\\o‘.1|sflv.\frn\aoun v.9.0n‘..hvw‘..x....vP ‘ .‘\..l4‘...-u¢ \OWUJ: VN. IS‘MHHQ. . “I!“ a IIIN“..H“I.L‘I\ .‘ a .4». 3‘10 3”“. 'I . . ‘\ Ih- nfio...‘ Yri‘ c .?I\ ~ v.00“. Vu’.‘ ~\ ‘1 u“.v \ ..‘N’ u. Q. t ‘us‘t. ...‘Aul- ..i. c). \flw". {\s H .. DI .‘v . E II. ‘\Uv <..)d . . ... ¢ . o‘\.so‘ v 9 \k”. .0... A .\ o ‘\ 1“. 1 as 1.. .p ‘Q {Lufxim- «\ .-.: «rim-- .- 1 .-.-a... .-..ruv... «3:13»... .- \ ..s.~....~..-.- .17... .o. v ... .I m “,9 . O.‘ . .. m. A.«..'. -.p.’ 9‘. Q-..9o.n ..o\..o o ...voL. .5 I. ii. I» .Nw..l «.oxvflu ...vOt 0.- )Jh .. . . . a. “I $.(v1q . 3‘ fiv.‘ .Q.\ \ ..- ... .... 3.. 35.-..“ . ... - ..kx... #5.». ...n. "my. . .. ..q..¢............-.)... 3...... . ... ....-. .-.. . ...... . ...-na- . .- 3»...- uw.m.s\...._..n.i. . . .. ...-.....- 1.}... «m.5.u..n.--.......n«x.. ..«.u......v....-m......- ...-...”...é .. «...-.... .... -u: -..... \‘vf. lolv . I t ‘ ‘ u ‘ . ‘0... I. .’. ' o'nu. .NO. u. ‘ .Qn. QC \O....'&‘. ‘5‘ ..I.~ o. 0.1.). Isl-«(K .. c $\ h u 0 s w“).\ .v. . ool\ ..‘.v.V . .o\ '0 "x n 'MJJ‘IYfiJFX . OJIQU. a . -o...h . "Hun“.xMWhuxno3fir. 5 {2-1-3an .. n..\..\\\!u.t.\..ohs I...“ .31... 90.“. .u id ”\VLIQ. ..Pk. ..- Nb“§.o.9u¢.;y uh“: [£6 0 .I) -1 . r. 4N5W....nuuh'hrs¢l\g~ \.¢H.‘\w~...."o 0’. ....hxflbfi.w 0.1:“...V0. h u ‘\ . .- . . . .. . . p . _ a . . u .\ ~ 5.“ 3’ Q o . 1 o \ - Ir. . .. . . - ... .. . .. . . . . . {\ f. .. \ .y \g r- ,. so . . . .t. . . q . VOLC. L3.- )érui . . - , . . . .. - . . ...-11¢.th ... .... .«fiufifidflxafirdwutl «.3... .. -....uyd. . a -..-UN. 2.39. ....w‘. in. .. .... ...- m. . . . - - . . . . . 3...... .... ...... Esra... ....»K 5...... ...-..- -..... - - -. .. u u . - . . . ..- . . ... . . -\\.s....\Myn.-.~oco1w.w).q! - \w‘K‘muufifis-f."u.f.c ......‘mwt ‘sflu. l... \u,\..... . u. .x V .. . ... . a - . - _ s . 1 oh . I‘D . u. .. . - . . . . , u . «Li-.3. 13!. . \Q . : . Av . - . a . . . c . A. . . .. . . . . . .1 fiQfi.«VI¢\Q.V.o. . Q \cmaVBE. u. v "ho‘. I ‘P..c. V .u . - a - .. . do.» ,‘n... \ 0 ouh(.‘..fhtl.r I .‘r.u ‘1- lw‘fiu. . .u . . . a Gilé‘ ‘ "ib‘U .0 60.0! .V‘O..O NU. ‘. _ |s\uflc.cv..u~i~.i\ .11” J 7 54.1.9». .w .r . . - - . . . . £13....2hy. .... ..rswxuwflrifl...“--. .... «NJ d...........v..»fi.s....-.-U. .. .. ...-... . - 3 ...smxm. ...}.-x‘nuxmd. . . .. . . . . - .. . ._ . ...-«v. -u.-\....\..~..v.!..: ... .- -. «aha-(...; 2L. .M.u...~...:. ...-(«w h-.. .... .... 77...-.. . . . . . .| O o . . u . n: .‘l'. .I .1 v x 54.31.. .r..‘....L....~E..S vi ...!‘Qufi. ”at. . . . - u .35 Q .. c .. . . .h...\. .. .. .... LS$I. .vx..-. .. ($3.-.. V... I“ .. c - s 1...... . ...-.1... "flaky-...? . .. . $5.2. ...-w......m........-......a....\.......-. . ....w-w....~.n.--un-.....» ...-(......- -... ....- .. .... .5, I \v o I . . i '5 u§rr ..N I T . . c Q Q... o. o 5 ¥ \V “ . {I} . j.- c .05”‘\I"N . - o .n ‘1. . ‘5‘“.- - ......c;I.0u\lW‘|§~ 9. Q V O\ Q. \orsit' D ‘ w. ...u......!.¢.v . “.25.... . n. . . Q! \ v A- . Q 04: ”V..H.N\c.f..fiafid. - I . .o. SIP.&.0\\\.« ....:\o.;\ .u \y‘.c..\In-u~. 00“... . . (W.WH.V“..JMQ~V. .{Qfioom o.I\s -...-4‘..tu.H.-o,. p.- . \. “Ur-Y0; J -4 9. fr...l..a.!l-. 0.. I - t . . - . - . . a . . . . q . . c n I o. . . a?! a. . . . . . . . . . . . _ . u. .. o ‘5. .. . \ n O IIIL . .. . - .. . .. . -o . . . _ hf“. \u¢ ... . . 8‘ v o \u v \- .Qw“. a \.o.u. ;.|. ... ’SW.- an..or1“fi.fl X .. I . h 0 v. H c. I . v I r. . . a II . . u v n I .u 0. I VI. .1 0‘ ‘vr‘n‘-W‘.,< .09 II... n ‘OHI '0‘. I 1“” O W.” mv‘o‘ldvhw t ..1 II t I ~ 0 Q‘ A. , . . .- . . .. . u. . . J - - , o . .- . . . . . . . I - . . .. - to»- ..P‘9 .\ - \ ....\ . . . . .- dim} dbrw, u‘.’ g o “I‘V‘ Q"\1vI-.\\“ V0.|H.V$\1‘o” ..».oo.le.$llI1.h\N.TCOa.u ‘ivt..o. 0N.-.‘u I I .‘nfl. ...-.....1. .\ f§\\.|($numouufla 1L. 3 . s. u... v... . (“..on .-P \...~.\LO 'fW»L‘§.um\l . “3.3.. Q .o.‘ M. ...“.o..- ..1. QVI.‘.\ . shun“. ...n..ha..r.._..1. ts. p; . 3...... -. ......39... ...-$33-.. ...nxs... ...-...... ... ...... .n..s-..--. -1.\t.o. 0‘ .. . I. ~ \. \...L... ..C. .s.... q) «\H‘.\. MMQ. .- l I 1n - UK... .9.- ..oc- \.\~‘v . - . .h I al’ “‘1‘. IQ.”‘II.£ 1"]- .t‘he‘x u‘ ’4' ‘.¥V~Q.r0.uc ,. 4| ‘. ..\ V o .. 1.0.1! 0" I‘|.-.~cuv‘9‘ t .-. .v 0521‘! b k k. . IVY. )I.0.. I... A: v .a '00 ‘0 \ .‘JV .9 5%!“ c ‘.‘ .‘PD’O c ‘.d C. I. ".. .Q ‘ o. ‘ o It. I '0... '0' C u .. 1.. o ‘ .‘ Jr‘.....‘ .J ."W‘l .‘0.. c o /la.0h.o 1 xx .. . (rvHWWIY...fl..O. ”My? \unflogl-r $9,? . , o 0‘ 5...)! I \(H . ..a . 4 I rung?!“ .I..- V. v . a . I 4 I but}? ‘3 4.43.: a... fh rd; . . .. . . . I. b(' l- . v on l‘anT‘QN). b ”w .lu....- ‘ .o LO. \H .\ c.53‘\ ,1‘xr ¢.... ..$.~\.fl\ 0\._u‘.on t a h” u Pol“: Q.” .\u-.O'v. at. .Ivcn‘LI-WMN‘LJ.‘ ‘ \oQHOOMPt. §u9.o Jump-‘1}... \NWQaJ‘oirofifl .r-..( 10‘... 9.0 . Is a. ‘v v [ETHWoJ tuill‘A 9"“;0‘ .INV 00 hi...- L‘, \.. Q. I: Q-\.v‘.n-h..\-«Q.un x‘1s' .‘0?sh\‘<‘c.’ L.v‘ .1 - I‘O..~ . o’Vvu‘u'oll’s r. t . 95“ a $.. .u.‘.v?fi‘t.qwm?r‘n.l§ a -0 . L1G...‘H.O\“.'I.‘.NO \..H...O..0It I \--h. .vo...l‘\|..| fl _- 0‘9 . . .-.: - .-- - - . .. $5....7: . ..2. ..-. . .-. o.\.-.- . vvlut AV .IlvlndvW4a. ‘l'n‘fl. - .J‘OOIou. .‘JQL’M q .. q . A . a . . a - o _ _ . 0O. .N‘ .. 1.. .u .o.~JF.I‘¢t 0.1.0.! vb \JWbusolLIV on ... . . ... .o-. . ..N .O&“’ .‘A ... to .. .lo\!\v‘. . firr..ui-n-\olrot. ...“...er . «FWfsn. . - - . -. . . - . . . .. .... ... .. - ,. - .- - - .. . - . t... 33...... 3....W-.:..v.w.-...2...... ...»...f.-.-...........t.... . .x; ... - -... ...4. -..- -.r- ..r. -.x- -2... . . . 3. . A. . - .- ‘ . \‘H...\6 .109. ‘00" 0’1 a u .H‘ ‘0‘.)0‘9' luau . . tu IND! up“?! A»... 1'.I."O\V“l.)l|u‘.).og..t . . - 9.3.... retail... o RS»... ..dflnu..‘Q\.’ YNIh‘! . \ ’I \ \D .0 v.\‘. 4" I":\-A’I‘1¥ " at. o a o .\ . . . .G' 1"“! 1 )q~\ ,va.,'4“.. I‘ ‘v. lu.\‘ .I a v“ ~K" \ .‘WIO. .. .\.\O. ' )0 k I.‘\V 1\I ‘\ OI VI . I i | . .. ‘ '0 gtfho} -.., || 1 ....(U . .c ' . v . A I -»5.‘A$.Q-§. 1.x.” 10“.“Ilh 10“ ‘. 0‘ o.‘ I . ‘.\ I. .4..’ )9 oo‘\ A‘ .0: 01 V ‘~ 00 In... \ 0A 0' I a . O O O O‘ \ n. . . tigh‘u ...I. I . - - u. . ‘. n : . . . -... . . I . q (“‘0‘ a“. 3 ’09“‘Y~\K\En\9c’.a ngu‘ ... \-q$\‘50.‘ ‘rno \ofic‘o-m .u. ‘I IQ. «u u...k.. 3..Q‘..V\'.‘l.'.‘ . . A 4- a . n . . A . . . u . u v . t O . u . o . u b . . . . o u . u . . ¢ . o - - \OAU‘\(.\|QMM. Q‘.‘ N Cal Q $'\.‘i‘0 K'Nn‘V . ‘ VI- ‘cx‘ ‘Q-q‘|-\ ~‘t. .-.. I u q . . ~ 0 n . . O .I - ' 0 'v. -. C .. u. - . c O . - . . . For... ,0 . O‘ o .. - .\ Vh..W..v.‘.-nl\ 60.v\‘u§.l .AI'AAcO‘ "to‘no‘.§ ”ha. .... .O.‘ Q ‘0‘ r1 r.’)‘t¢§.t¢‘hfl‘a¢\ "flflCxt \ 5. . ..y‘.‘ I O «I. 'I7 . . V.) M'fllo- . . o..¢‘§.‘nw}u\‘ai..1 \‘Q‘Jo I 7.: 6.05%. 3‘90. in..~.—...O u ..1. «in. .I‘ifn- . .. ... NO’\I\.c .A\.- \u ‘11... 6.0!! Jv’tflh l5.‘ . I ' 0“. ‘v‘\ \ IV.\ ‘ ~‘fi. .. v a . o..‘0rl.'r\db.‘l. VA ‘ a . C- ...uvort... . ...u 3%: U; . 21$... ifi:U-U1.A..Ju.os.nn3%ubnfiuflfrm- Vb .. . . navy-re. turn-3:...Q n.....\.w..~n......\1..:b.... ...s.............. x ...: MA...» .....x . .\......c. -. l ‘ ’. .n .. .d . Gd A . . . u I I I . .u I . . Q. .‘1 . ' I a. J.» o 3? 1.! ins. (.1 93...»!- Kv . ”Nab.- ..:h .19. . ... $3». «...... ...-y. ......R f .aw..k..ma #- ha... .../....xu... .. £1- . -.uv .. \.\q| . . -..-.... - - gr ~1‘... VJ§\;~ 1-“ 5| VH-“unm.l§uv ofixvofi..\anmn\w‘uv\‘qi” .‘\ 13 O. |\ 8 15‘ I .- ...3u MI L v\ as“. t &!t\ . x In. 1.‘ 1 J U. u . ¢ t ‘l 0 I. {.u - 1‘0 1 .u ‘ . a ' | v. I '.I" . -.. .. Jane‘vt... (My. ..vdh; .- 0% {Vi-uh. ..\\o%t\y t1 s.-|\ .M «x \oto...‘k.u‘¢ u‘.‘ ! 1 -.u..\ v -.. o\r0 . u o . o 4 ‘9. .ur, P .n 13‘10|0N:“ .. 60'. \l ...-o. . CV b 0: I...‘ I.\‘.Jtlv . O Ol.vo VI... I .... 00'.) It I .a. O. . ...“..Q.‘§\fi¢oflu’§tfivt) . ..o I" “inn . $3,.» ”‘ ..IUUU. M‘ u WI\\ \L .I|0¢6faw\ .‘IV! hu.w4.\. . ... a . ‘ . . .0. \0‘1 . q .I o . u\‘.v .\.?‘0u‘ O V “V: '0 ... .‘GQ‘II‘OK o -0 Nhu.‘ In‘.|\clx \oa \II t|u$l. Q? I..‘ h! ‘ QDHI O. t . -.. ' Y ILI.‘ ‘. _ N.#.~h"$n 69.1.)...“ .o ..-.I... 3‘.“ I a. b1vvv-.o.uo .Qw.-.I.. ..--O 9... {Ottakv‘u‘u o. o. l -5 o ‘3 ' . I 0 co 0 .... .l . .( .4‘t .U 13.8....725 .09 -. Qt? 9'. .. ...-x... . 4.. A .Q. noo.o..u. Va.“ 50“ 6. \.-.!. I - . . - -. v 1‘ ... \ .a- o . \ . I Q g u I q \ a. o. . . . . 6s! . U Q. t. n . - v :J‘Dotkt O/HIvvmth N‘Hfirtv‘.v.0..-v‘“.l\.qu‘v.Po-‘WM‘hHic‘l '11“- nu. ) ano‘ol. .-D‘ nu»?! \Loo§.-? .‘ v0.'u‘- . «bk \' 0‘ v . 05 | » ... N... ...”H \II \ . | ..o" w.. . .l.|\ ..u t -u . .‘ C .\\..\v .QON.§..HV~ 2! .mW! H.120"...- (o ...lo \ \\Hc-\. .. \ V. v ... .\ Jrv\ ‘..o o. ‘\..O 0 K.. c‘:.” Icl'G... \ I. o 1.3-.S7fini \1.Il.¢|\\. .N.‘ .hb‘.\.\\ .- ...-s ‘vl. Liu.‘..0 u . \\ ‘~bfl1 loco MO“\C . V‘L ‘o." . III‘I.‘I'9 .r . J . . L. t 1. -¢ . . . . I . . ... ....v \..o.‘r\.0 I... gflv‘...b Odnohu ....-\\.H.W.-.ofl.r.-....9OH.OW~HII.J l \NHHKHHW. oil ‘IulrI ... o \la. .... It”... \I. .0. \ovr I..- .‘ ...!a. . \hk'\‘.\u‘\'¢flo.. ‘cI‘IWIO-u... . H... nt‘ct. . . ¢!a.l ‘\\\\\" . $u\.v!‘\ ..-. 0‘ 01 \-n .0 V.‘ I an . nun ‘Q'~|«- O‘I" .§~v\'. ‘I‘Q . 10-... -.‘..I\ _. C KVCOOQV... ‘ . ‘1‘. ‘ Q . . . '- . I p'q . .5 .\J Q ‘0 00‘ 0.!9.l.vlal.‘ A.» , ‘5‘ \~Ol‘lll\’ t .\ c r \‘it . 1. § . c . v A . C II I. . .. c I I \ .\ I ~ .. o “\o . .nfiwh. ’0..\ fl .9 o to... 0 Q! q \ .u. ‘ ‘0‘ \O’K.’ v. D I I. \L II C. c h II .Q‘ “ ‘ . ¢ - p r p ...on | D .0 ~ ‘ \\0.._'lt‘n’0‘c O . 3‘..ch .‘ .1!“ ‘V- ”.O‘ L "H‘ ‘ v Q. U... Ill: 5‘.” I. .“1 y“ K-1150.0;'I ‘ a ..I o. I . . 1.. . - .o . o . .. . c 05‘ . .... . . .--. .\ C40 i u 0; . t! n .-.-.. '5. u o\ o. o - .--I\.I.. . D ; I I.‘ . .| . . . . o I . . t O . o k 1‘ a..- ‘ ...“ a '- Q o .n‘. ‘ N o.\o . a Q. 7. 3.111131 Km...&1-flrfl1wvflcouim¢ r Irv..-.ulvic.\$h§.u , a 4m 9 w I A ‘D A . .L . u.‘ k w .‘b o I ...: I .r s 5;. Ir... . a . 5-. w J , .. .‘k K O I L ‘\ 5'0 - 3..‘.?3¢343uw13u25 .....Aji4a4444111 1 114.114.141-112”: 11.1.2411“: E_-€wfi.¥yrEW-lbu¥wmwru: . ; awkwiymaflmmh”MwDfiJE.-.».FQ 5.3.4.. ......tr... n -- . " 1. ' v" .' . .- , - . ‘ g 1 ‘ -_ 0.. '3‘ ‘ ‘ F . .. ‘2 :75 ‘(f "(.'*;‘:§fid$ {".~(",I-': it; . - r n '0’! ':lg # ' . ' .1 - ‘ . .. .V . . - . 3‘; " $., If,',’ ig."‘.r‘:. ’ y . ' \: mc.‘¢ ' . ¥ '. " . ‘ I ' “~‘ 1. :. ‘I‘it _ g ,u _ . .. , .a '7 _ . . ' ' . ‘\,‘ f. u‘v‘rr . I.I"‘".y . .‘P 1, \’~ . . - f 1 ...?~-s=7*-w . ,-.»-~:r....;g,.-,. '- ’1‘ ‘ ‘ f ' mm " aw” ' 1 w I 1 , . 1 -.. ‘i( n :7; ‘ '. 'Q‘f‘ll"'.,r- V . ‘ .‘. - ' . g 1‘ .1 ' . "'1‘"; v’...';,_ .9 {f’aq 7m\ .-(t:‘ P_ 1‘ . 1- 33" " ”Jag {51 p f ‘ ‘ .'5“,_ ,:. Vf',:. ’z ‘. ', . » 2'.'- . ."‘" . ~- .-1 'c",1 "'5' - l . 4“» ~ . - .1...- 11... .».,- _ . ~ -. w. « . .. .111 Mae-:5", - . ~ ,1 -..‘-$.;'«"~"r,s. ' - — ' ' f- ' ‘ " .1 ' ' ‘ - . ‘ : 10: r ' 141315153. ".11“.th Dl‘, - ..‘. ‘- . . _ « t ' ' Vl- ' . ,". “"4’ "‘ ‘.. -0 -.' ,. .- . . f ‘ ("HI-(3‘; ‘:“A"‘"3é."'jh<.('n‘J-fl‘ 1‘“ 4 9'”; 1‘ I "' 3‘ 1 A: ... . '. ' ' 1 1. ; .7 I "‘ .1 . l '1- 4 ‘ | fi. '5 I} .l o 1:» ‘. ‘ .t‘t‘! J .', ‘1 M 4 r- ' {jg . .,. it? J $' “ 3 {’1 . , . ,h ,. . fig ~ 1‘ £111.32». "6 ""33 .1“ "VJ . up; .(4 . , o v .' .‘ l . :‘ .3' » ‘ 1 ‘ " r 3"», "12’ it»? .4 r“; a» ',f,»'l.:‘<;.;\7f 5 ~ ~~ .1 . ;* .w 55 \ i P, . ‘ , .. $ 5 ¢ 1 1' ‘ ‘4 . 1 ‘ ‘03., .--“, :: "’&¥‘~; ”'1‘”: 21; 1 1'3 'VV. 7'30? 1‘3"}. '1‘? . "" . Q' A ... 1',_‘ .4 ~ "_ I ‘ _ ‘ I." ’9‘... » "fif’l'fi‘ ”i\ h. 6 "I L ’3 ‘ r , 3.4-“. . ‘g‘ 0 w ”+1., \ 1‘, R ’1'3’ " ""1‘1 .‘;) .‘ " :flr k“ 7 5 I! ’. ’; ~,; .: '0 ..t‘ 'v- ~~ 5, "Y“ . 3" <- ‘ r a ‘9'":- “ , r a.‘ 9|:- % o. .b" ~ ‘ i. ‘W n. f ~9) "V ’.".v’; v ' -. v ,1. , .u; - m; , 1, ...». 4b r ‘l’ I.‘?.* ‘0‘ " I" 1K5 ' l 'gvll‘“ I ~ '. ti. .zt-fl‘l‘ ‘-1 . ‘ ‘..C‘ 5 ’ t ‘ l "\ ‘ U)’ ‘ ~ '1 -“‘1‘ . .f" A . I. .. _ - ‘v.t.'1 Q. "(-r“ '3, u 4 (. 9,‘ H121” . .y - < ',_. ‘ . ,l 1 q 914$ 1- . o (1.. ‘15- ' ,r, o v r *y. 4" M" K '3 .k r ‘y ’ 'L 's- " .fi... L, 1" ‘ 3.33 ‘ "Vi“;u _\ ‘v. .u a .$ 3 I [a '. 7‘13““ a $11 ‘1 4‘, ‘ ': . ‘Q '- "' ' ‘w‘t _. .c 'n ' ;‘ ., 7 """ ' ‘ . ‘_. . "i\“ at fR-fiw'yf'f ‘v‘ '. . \‘I P, ‘r I . “‘ ' '. ‘( . ‘ .1 . ~ ‘3 ‘3 i ‘1 ‘ f‘ - .1 H .';‘.--,‘fi 1 L ‘l‘r. .. 1 " a; :EJ.,V:\; “' ,.’f . ' . '. , , ’\‘ t ,1: -'1e-:-gx«.-$ 3,3 t: ”12:." 14..» L A ,1 _ . . :., ' . v 1" ‘ ‘ 4 . ‘ 0 ‘ I . ‘1 - ‘ H ‘9 . I ‘ ( t ‘4 ' ¢ 5,. u: 0 ~ 1. g, ,1 S a.» x‘.‘ ’1». .1 I c ’ '.‘ *1}. v .‘ ‘ ‘- ‘i L . 1 , . .' ‘1 r' g ‘ ' 5“)J,IJ.{.\\- ‘3 ’..‘.. ‘ 3 ‘ ‘r .' '0' A." y W. I, ,1_"" v‘,’ ‘ (f ‘ “ ..,' -'n o ' - v‘ 3“ ’43“) '. n \ ~—/‘?‘c(’.ho‘.1§1 -' t- ,E '; , - ‘. ,v-‘ .1551" my» fl . "'3”. 1!. 1 ',‘ .‘ _ ~'p-' {1%} ‘J\ 1 x ' , :l “*‘I' ‘3“ 1’ t‘ ’. f ... ‘ k_ "(4 ~' .. 3" " "'1 I, I; ‘L ‘~ ‘ - . .H r 'g‘; !.-.k‘... .‘lm l O - I ." . . ‘. ' . t ‘ 1': 1V ‘, ‘ ‘ ., x {7‘1 ‘ n,‘ '.“.' - § \I“ 1 95K?" »_.~..*_2. I 1.3+- -‘-' ‘ “ ‘$V . 1 ‘ 1' J."..'°. a. ' ’ 5 "l V‘.’: v N ” ‘ ‘ - 'I. . 1 ,, W -‘ "'V "1.)“ 3.- “"». . .51 0133‘s. - 1‘1".» 4-9.9 'I‘ {'n t’ 0‘ ... - ' ’ ‘ I ' | - \ If \ 4‘ ' v I ~E .\ .. ,, .J ‘. - V' ’. ’ q 'U 3,) *3 ... .y o & ‘ . \L ,. ' ~ “L ,., '_35_..‘.:, _ - 1‘ ’ . r ‘:_‘._ '. ~_ ’ ,_ 4 "'vrw»1&'*"-'.* 3'”: V“ V 5 ' _ '. _ l -vl: 31"1 ‘L|‘,"’J ‘ " ‘ \JRV .'\‘. "/. I, 3 “.p 5. I I. ‘1“? ‘J’ ..V” ‘ "Q" NL-«T - ~ 35. -.",T ‘52".. 7 ’ ' ”.3“ 2"." .1 .‘ 5'13 "..5" "1:33;"? “I". 7353‘:- Q'I‘bg' F l (4* 1‘ "'75" 1 ’ 15>: ‘ ‘1‘ -» '3‘ v 4w- :«2». ~‘-.'»‘ .5‘ -":-..=»r ‘4‘ ’ 19’»; "‘ . a 2“,“ 4.-‘.‘,"c‘. ‘mf‘u‘g". “wrap. " '- “.11." ’ 13-53; "1 vhf» : 0"")- «*‘u- “1.";1. L 1-2 "~‘<~1.=-x-'u ~ Y ‘3'- 5'" ’h'fiubg1l‘éfx " ‘4‘zm"“ '1‘. d 1“”Vr‘ a, ‘(. ‘ ‘QIC U"; 1?: .’ 1 ’. ‘ '., . _ . 6.3.. _ g, ,, f p' \ , , '1' ‘~ ‘ .. . w - 11; -; ‘5. .. 21%.? 910‘”er 1:14.. . .. ..“‘." 2‘? 1&1 ‘3. "'93 A . -. .15.» 1 ‘h ' r ”ff? "5"“ " "" “ ., ' ‘v' ‘ «‘1‘» "* w-‘4-‘h' ‘3 Mo 1". ‘ r a'-- . A. V Izqf‘ f1".';‘n :‘ ‘ '1 I.""J . . ‘7'": ‘.‘» ‘*\§"Yl 4. 1‘ ' - V " ' 'I . ‘ A; 4‘ V , l ' 1 - -‘.' h » 1'-" ‘- it“, ’ ;f‘l1“ Yfi ’ rl 1V'- g ~.-‘ . r . ‘ -‘ . V v ,I" .1. r.&,73\‘ L‘}:"l »‘ If; .... . ‘I!"3-1,:'. ..1 _ré 4‘ 'l' ,3 '~' .' ’ ~ .I f ‘- vf'i. ‘1‘ “ " , 4 - _, ‘h: ‘ . . ; ' éld'--{(JL ' ‘>""'.l('\ ‘3 ‘+§ fl '1 ‘ .. .' ‘“‘$; .‘- I II | . I n h . . L ' I ‘ . '4 . . . .4; . I _‘ ,, ‘ . 4‘ or {k f l . is. l ° 4“ afi’g‘ ... ‘ . '- . " . S It '. "I ' ~" ”I “ ' ”1% -- ' .1y‘to")' "1".» '-~‘ 55' ~ 2-"- .‘ i‘ . n : I . ' , - -" ' ’ . 'l . - ' 3 ' . " ‘ b'e¥‘:*r'fi" ‘bJ #J’Q." J I, I "1.?“ ...? ,A L"'-"":" 3‘ { “IL . ' Ln") ' A ’ v. "ES‘TKSL. - . : ‘ 2' ‘. - ”“' ’_ “‘ W1 ’3'}; ,, .1 9} ‘ J . ‘y L?V:.“'~“: , . ' . ' ‘ ‘.- .‘1- . ,' ‘ ‘0' ‘ . ' ‘3 gfi-J' I ‘\ ' . .I, 1"” ‘ 5.3". ' ..f h, :J .w‘V} ,- 3". _ .. . - I u q “-53.34! {“9 5' ‘iu . f‘ ' .24.”... ,4 s1P'-§z’f"s¢k"!\~‘.‘ ' . ‘ _ _ :.~.;,'."-¢‘ . 4-7?» "1‘“ :3 -' ' 1-3. .- L. .1 :5 -; .1 .-‘.'-."i:" r'~=~‘£»_'- . - 1 w.".‘ ,. - ' ’5‘?“ ' paw7",‘21'3-95211'wvflfl ‘11,...i9fipm. Y - -" 1} i‘ . . . L 5, '1"! L: N, '_1 L ' _ , ' I“:‘; ,r 1'.- .. “3“ 5‘ - . . . . ~ 1 131.13,:71Kv1r ->'»r'-t=:”~o¢r’:- ' U ,; :33?» ’* F. 1.17%»; 11».J"-?-wu.-!qu:t. 1‘$?*"2,133_."1J‘r . .' ~ ‘ - ‘3. 3 3 " "~13... l, " 'VJ- ’ .» "3‘th b: - a .. ...iru . 3-1-3. * 0t J. ‘ I: - v ; ". ... ' ‘- .\. . . 1 r a .v v o u o 1. 233-1; 18.5”. , . . I 3;" 3: 1 '- : ‘1 ' .7 .- ' I I. -":‘ . I ' ' “.v I I‘- ' ‘I' .. ‘5' ‘b v'-: ‘ ‘ ‘ ' . "- ' AI’; "v - i ‘ 1 ‘ .‘M 1‘ A .‘ A: I . . I 'V. “U.h 4“ '. It ‘ .ft' N " "1-". . " . ‘ .- ,. 5" ' ..‘._ 1 ' ' 71‘ ‘ " '4 ¢f _? "' I‘. f . ‘. . .' n_" ~- . I .' ' m ‘ .. ,' . . 1&4” ‘3‘ ,H . '- ‘ - ~. V‘ ‘ _ ‘ '11. 0‘2 ‘1’»4» he)... 4,}? aw 1 ~ -_ i321“. ' " Wait} «a? * As? 5’ T3“ . 3‘ I! 3 . r {é’ ..., 44’5‘1‘ _. THE DPUIDUA bfifinLIS BCVIS. (A Seroloaical, Bacteriological, Anatomical and Pathological study.) THE DECIDUA BASALIS BOVIS. (A Serological, Bacteriological, Anatomical and Pathological study.) THESIS Submitted to the faculty of the Michigan State College in partial fulfillment of the requirements for the degree of Master of Science. By . 1 Edwin R3”Carlson we June 1927. \. I ., ‘ 1 l ‘ -’ t I THESKS . ‘ ~‘ ' '."J_ ‘0 “‘1‘.‘ CONTENTS Introductory discussion of problem. PART I Serological studies. PART E Study of fetal membranes. PART If! The Decidua Basalis Bovis. A study of the deciduous nature of bovine carunculae. COIITEI‘E TS. Pa5e Introduction.................................................... 1 PART I SerolO5ical Studies............................................. 5 EXperimental Data of Serological and Bacteriological Studies.... 4 Key to A55lutination Test Tables................................ 8 Group:§. A551utination Test of Entire herd Arran5ed According to Age Of AnimalSCOOO.....OOOOOOOOOOOO......0.0.0....9 Group :1} Agglutination Test of the Original Reactors Appear- ing in Group I that Remained Positive Throu5hout the TeStOOOOOOOOOOOOOOO00000000000000000000000000.0000... 14 Group 111. A551utination Test of the Ori5inal Ne5atives Appear- ing in Group'I that Remained Negative flhrou5 nout the Test....................ODOOOOOO0.0000000000000000... 18 Group 2!} Agglutination_Test of the Ori5ina1 Ne5atives Appear- ing in Group l'that Subsequently Showed Slight fie- actionSCOCOOOOOOOOOOO........OOOOOOOOOOOOOOOO0.00.... 22 Group:z. A55lutination_ lest of the Ori5inal Ne5ztives Appear- ing in Group 1 that became Classed as Reactors and then became NegatiVCCOOOOOOOOOOOOOO..........00...... 26 L3 Group Agglutination_ Test of the Original Ne5atives Appear- ing in Group 1 that became Positive.................. 29 Group VT . A551utination Test of another Herd Compiled to Deter- mine or Show what may possibly be the Sequel to the Post-Perturient Reactors in Group‘21................. 33 Post-Parturient Reactors of Group V1 . 05136;thBCDOcOoooooeeoeoooooooooooooocooooooooooooooo 34 Case #LI BDOOCOOOOOOOOOO......OO...00......O0.0.0.0... 55 Case fihl BCFOOOOOOOOIO.O.O.OI.........OOOOOOCOCOOCCCOO 36 Three Year Monthly A55lutination Test of Group VTT'Herd......... 37 _ Page Group Vil . Family Breeding Records of Animals in Group.l with Reference to the A55lutination Test of the Dam and Her Progeny. Also Breeding Records Accounting for the Females without Tested Pro5eny in the Herd..... 4O Sub-group 1. Positive Animals without Tested Progeny.......... 41 Sub-group 2. Ne5ative Animals without Tested Progeny.......... 43 Sub-group 5. Family Records of the_2§ms with Tested Progeny from Group ll, Group All, Group E, and Group E with Reference to the A55lutination Test of the Dam a'nd Her ProgenYOOOOOOOOOOOOO00000000000000... 45 Sub-group 30 Wont.) Family Breedin Records of Absolutely Positive Dams (Group YT with Reference to the Ag5lutina- tion Test of the Dam and Her Progeny............. 46 Family A55lutination Tests of Group TT'Animals No. AI, DB, E1, FG, co, HF, 10 and 137%.”... 47 Sub-5roup 5. (Cont.) Family’Breedigg_Records of Absolutely Negative ngs (Group All) with Reference to the A55luti- nation Test of the Dam and Her Progeny........... 56 Family A551utination Tests of Group 151 Animals N00 CG, DA, PB, G0, ABI, ADA and ADHoooooooooooo. 58 Sub-group 5. (Cont.) Family Breedin5 Records of "Dams with Slight Re- actions",(Group E) with ReferenCe to the Agglu- nation Test of the Dam and Her Progeny........... 65 Family A55lutination Tests of Group 3:! Animals No. CI, DI, HG, HI and AEE .....OIOOOOOOOOOOOOOOOO 67 Sub-group 5. (Cont.) Family Breedifig Records of "Ne5ative ngs that be- came Reactors , (Group 2:) with Reference to the A55lutination Test of the Dam and Her Progeny.... 72 Family A551utination Tests of Grou VI'Ani a No. AEI and AFH p "' m 18 ....0......00.0000000000000000.... 74 Page GroupE. Imlmne P‘amilieSOOOOOOO00000000000000000000.0000...0000 76 Family A55lutination Tests of Negative and Positive Families in which Abortions have not been known to have OccurredOOOC......O...’0.00.00.00.00............. 77 Summary of Serolo5ical Studies.................................. 81 PART g Study of Fetal Membranes........................................ 82 Introduction to Study of Fetal Membranes........................ 85 Experimental Data..0.0000000000000000.0.00000.........OOOOOOO... 84 Group I Part A. Study of Placental Arran5ement upon Fetal Membranes... 88 Placental Chart of # ABH............................. 89 ff!“ BAG-oceanooeoeoonoooooooeeooeoee 94 " H H #rfl BCHOOOOOO00000000000000.00000000 97 n H II # BOBOOOOOOOOOO0.0000000000000000. 100 " II n fle BEI.............oooooooooooocooo 105 N H N #M BCFQOeoeoocoo-000.000.000.000... 109 3 :: :: in!Ell-Fife.a.ooeeeoeeeeeeeoooeooeeooooo 113 " ‘eooooooeooeoe000.000.000.000. 117 I. l. 'l .. ~ .. ii S§:::::::::::::::::::::::::::::11% Group I Part B. Study of Accessory Uotylcdons......................... 129 Placental Photographs of # AFA........................ 150 Placental Chart Of # ACGooooooeeoeo00.000000000400000 154 " " " grid BDBOOCOOOOOO.......OOOOOOOCOOCO. 158 A...Boeone.oeeeooeeoeoeoeoooeooooo 142 u N H # ABC-00.000000.ooooeooooooooooooo 146 " N N #311. BG-aoooooooooeoeeoooooeoeoeeooo 150 GPCUp 1 Part C. Study of the Presence of Naked Areas upon the Chorion. 152 filacental g}1art 3f : AIGi'gOOOQOOOOOOOOOOOQooo0000.000... 153 0000000000000000000000000no... 157 Placental Photo5raph of #H. BG........................ 162 Group :1; Part A. Study of Fetal Membranes from Cows with History of Disease Prior to Last Parturition................... Placental chart or # AFFEOOOOOOOOOOOO00.0.00000.0... Group Part B. “31 Lesions seen in No 51a icental Chart Of # BOHooooooooeeeo00000000000000. Part C. IHI Group Diseased Fetal membraneSOO0000000000000...000000000. Placental Chart of # Group :1: Part D. II N N I! I! # GEOOOOOOOOOOOO0.000000000000000 Pmal Fetal MembraneSooooooooeeoooo #101BEGOOOOOOOQOOOQOOOQOOOO0000... AEIOOOOOOOOOOOOOOOOOOO0.0.0... 7f BACO.......OOOOOOOOOOOOOOCO... W11 BAFOOOO......OOOOOOOOOOOOOO... # BOP...00............OOOOOOOO... 1f, AEAOOOIOCO.....OOOOOOOOOOOOOOO fféHo CCAOOOOOOOOCOO-OOOOOOOOOOOO... # AGAoeeoeooooooeoo0000000000... Study of Fetal Membranes from Consecutive Pa rturit- ions................................................ Consecutive Placental Charts of # I! H AEO.............. AFB.............. #M BBGeooooooooeoee. I BOFOOOOOOOOOOOOOO Microscopic Study of Interplacental Lesions seen in Diseased Fetal Membranes................................................ Summary of Placental Studies................................... PART TIT The DOCidua Basal-is BO‘Ii—SCOOOOOOOOOOOOOOO......OOOOOOOCOOOOOOOO RCView Of Literatureoooooooooooooo000000o0.0000000000000000000. Observations Concerning the Evolution of the Maternal and Fetal Placentaooooooooooooo0000co0000000000000 Pa5e 164 165 168 172 173 177 180 181 188 195 198 204 211 216 222 223 254 245 255 265 268 269 270 272 Page Anchoring Villi during Placental Evolution....................... 274 Changes in Maternal Epithelium during Placental Evolution........ 277 Changes in Uterine Glands in Early Stages of Pregnancy........... 280 Structure of Mature Maternal and Fetal Placenta.................; 281 Study of Placentitis............................................. 284 Study of Retained Fetal Membranes................................ 295 Clinical Proof of the Deciduous Nature of the Bovine Carunculae.. 511 Macros00pic and Microscopic Proof of the Deciduous Nature of the Bovine Carunculae............................................ 527 CODClUSiOn O O O O O O I O O O O O O O O O O 0 O O O O O O O O O O O O O O O O O O 0 O 0 O O O O O O O O O O O O O O O O 535 Bibliograplly . O O O O O O O O O O O O O O O O O O O O O O O O 0 O O O O O O O O O O O O O O O O O O O O O O O O O O . 556 ACknOVJledgement . O O O O O O O O O 0 O O O O O O O O O O O O O O O O O O I O O O O O O O O O O O O O O O O O O O O 337 0011313611311160111110....0.0000000000000000.0.0.0.000.00.00.000.000. 358 D?-ICIDUA Baijlel S 11 V13 II; THC" Db C TI C-‘IJ A quotation from an editorial of the January 1927 issue of the Cornell Veterinarian Oives full extression to my personal views of research. "One is tempted to comment on the manner of birth of medical literature, or the importance of separating tie tFeOIeticsl fron he real, the creed iron the proved. A first hand stray of dis- ease as it occurs naturally in large domestic anirals is an arduous task, but it leads to the main highway of truth. Philo- sophy and exnerir‘lentation are in iswcnsa‘ole, yet they must com- municate with the main channel." The purpose of my work, recorded in this thesis, is to tresent data made from a first hand stony of rhysiological are patholo¢i- cal processes as they occurred in herds under observation. It is hoped that the data rresented will aid towards clearing an the confusing and conflicting statements found in texts and current literature concerning the a1atony, nhysiolon and path- ology of the involuting processes of the bovine uterus. It is hored above all that tPe data will give a practical un- derstanding of the puerperium that will lead to rational ratler tlan impirical treatment of retained fetal membranes. I believe, that in order to treat a cow for the rrtention of fetal membranes, an urderstancing of the r7ysiologic l frocesscs is necessary. If the writings of one observer 1 ads him to make the state- ment ttat the car1.mcul.-e are not deciduous and that tine lochia seen during puerrerium is nathologic rather than physiologic, one must naturally have a different undvrstandind and line of treat- n at then if the carunmrlre Ml d<:ciduous and t1 e lochia of puer- perium physiologic rather than rathelogic. ‘ If the carunculae are deciduous, then if lesions irvolve this tarticular structure the dequel would not be so serious as would be the case if the carunculae underwent involution with retent- ion of the characteristics of the structures. If lochia is not physiologic and is to be interpreted as path- ologic when it really is physiologic as a result of deciduous carunculae then the quack can get in his successful treatment of "pyomtra" or the well meaning intelligent practitioner can be mislead to treat an otherwise normal cow because of the pleas of the owner that his cow has a "dirty discharge." In this thesis I have recorded extensive serological studies of the herds under observation, I have recorded charts of nor- mal and diseased fetal membranes and I am presenting clinical and microscopic evidence of deciduous carunculae and resulting lochia. The serological studies were made to determine the type of in- fections and the cause of the lesions seen in the fetal membranes. The fetal membranes were studied to determine the significance of naked areas, accessory cotyledons, diseased placental and inter- placental areas. The lesions of the placental and interplacental areas were studied to determine what significance 8 diseased area really had. Williams states,2 ”Any disease process occurring upon the placen- tal or uterine surface of the chorion are duplicate in character in the endometrium. The records of such lesions are as indelibly written as though chiselled in marble and once disease has invaded the cotyledons of a cow the proof of such changes will be authen- tically recorded on the fetal membranes of each conception as long as sh: breeds." "A uterine cotyledon once destroyed vanishes for- ever 0 Observations are presented in this thesis that give a different viewpoint of the ultimate termination of the carunculae; a termi- nation that leads me to state that the bovine female has what is equivalent to the decidua basalis of the human. It is for this reason that I chose the title, "Decidua Basalis Bovis' for my thesis. PART 1 SEROLCGICAL STUDIES. PART g STUDY or FETAL tarsal-tress. PART III DECIDUA BASALIS BOVI s. SEROLOGICAL STLDIES OF HERD UNLEH OBSEIVATICK. In order to determine the age of lesions seen in bacterium abortus infected fetal membranes, a monthly agglutination test was made of the herd under observation. Whether the test is absolutely accurate in determining the time the disease starts, I am not going to discuss because I have no way to prove or disprove it in this short period. In tabulating the monthly agglutination test data, many ob- servations were made besides those sought, that were apparent- ly valuable enough to record. I have therefore recorded the entire years test in the thesis. In compiling the data I have made the following groupings and sub-groupings. 1. Test of entire herd. Arrangement according to age. 2. Original reactors appearing in chart one. (That remained po 81 tive e) 3. Original negatives appearing in chart one. (That remained negative.) ‘ 4. Original negatives that appeared in chart one that sub- sequently showed slight reactions. 5. Original negatives in chart one that became reactors and then became negative. 6. Original negatives in chart one that became positive. 7. Record of another herd used to determine or show what may possibly be the sequel to the post-parturient reactors in group six. 8. Breeding records of group one with reference to the aggluti- nation test of the dam and her progeny. Breeding records accounting for the females without tested progeny in the herd. EXPERIMENTAL DATA. Group Two. (Original reactors) ' This group of animals had reactions at the start of the test that classed them as positive. A study of the accompanying chart will give an accurate idea as to the status of the animals 0 Group Three. (Original negatives, remaining absolutely negative throughout the test.) A number of animals that were absolutely negative at the start of the test never showed as much as a trace of a reaction during the entire year. The number is approximately equal to the number that started as positive reactors. In a study of its accompanying chart the absence of history of abortions are to be noted and under remarks the non-specific infections are indicated. . . u v .. .. p. . . u . u~e . ~ .» e . ... .. .. I. . . .... . . . .1. . n \ u .- .. u . . .l- . \ n 0 . d \. . ...-O at. ... ... e. . C I. I I I e . '0' 0‘ h IN I . . -. .ul . . t . . . . o 0 e O o . . ' n . . u I o . J I Group your. (Original negatives, that subsequently showed slight reactions.) The facts to be noticed are that history of abortion, his- tory of retained fetal membranes and recent abortions are greater in this group than in group three. Group Five. (Original negatives that became reactors and than became negttive.) Three animals had this type of reaction. Note the accompany- ing charts of each animal. Group Six. (Original negatives that became reactors.) This is the group that reliance was placed upon to obtain fetal membranes in various stages of infectious abortion dis- ease as signified by serological tests. Some were obtained and recorded. The interesting point brought out in this group is that five animals became strongly positive following a normal parturition. In order to obtain data that would throw some light upon the sequel of such cases I tabulated data in a herd that had been periodically tested since March 1924. This herd was negative at the start of the test. (360 Group Seven.) There were three animals in this herd that had been negative and became strongly positive. These animals have never aborted. See #MBCF, #MBDO and #MBQD, under group seven. Group 5even. Record of a herd that was negative three years ago. Data was used to show possible sequel to post-parturient reactors that appear in group six. Group Eight. \ An attempt to correlate the data of the agglutination test and the breeding records of the dams and their progeny. Consideration has to be taken of the animals without female progeny in the test records. Consideration must also be taken that females may be without tested female progeny for the following reasons. ' 1. May be a heifer without progeny. 2. Progeny may be a female under eight months of age and therefore untested. 3. May have had male progeny. 4. May have aborted. 5. Progeny may have died soon after birth. 6. PrOgeny may have been sold. 7. Combinations of the above factors should also be con- sidered. Group Eight. (:Ebip. data continued.) Three sub-groups were made of this grOUp. Sub-group 1. Positive animals without tested progeny. of group eight. Under this group all positive animals are con- sidered and their particular grouping indicat- ed, showing their relation to group one. Sub-group 2. Negative animals without tested progeny. of group eight. Under this group all negative animals are con- sidered and their particular grouping indicat- ed, showing their relation to group one. Sub-group 3. Animals with tested progeny. of group eight. - Arranged in their respective groupings, showing the particular group they came from. Discussion of the three sub-groups of group eight. In a study of the summary of the negative and positive animals without tested progeny it is interesting to note that each group had an equal number of animals. No. of animals No. of parturitions No. of living males Calves that died Aborted calves Untested females Sub-group l of group eight. 14 46 15 10 (lmale 9 females) 15 4 Sub-group 2 of group eight 0 14 43 16 17 (5 Males llFemales 7 3 fit '- ‘l. I ‘4. 4 In: - 9 l_fl Expo data of the three sub-groups of group eight continued. Sub-group 3 H v-. .“m‘. a -t, of group Af‘illfili S N 1 .31 T his: “14.) i’lth’Ll; Y o eight 0 Group E Group ill Group I Group El: N0 0 or animal 8 o 9 7 5 2 No. of parturitions. 68 41 55 4 Untested females. 3 5 1 1 Normal deceased 5 3 4 0 females. Normal living 25 14 12 0 males. Abortions. 17 6 .7 O Neg. daughters. 4 8 3 2 Pos. daughters. 13 1 2 0 PO 8 o to 3118p o 1 2 1 0 daughters. Susp. daughters. 1 O 2 9 Neg. daughters. (Grand) 1 O O 0 Pos. daughters. (Grand) 0 0 2 0 PCs. to Susp. O l 0 0 daughters. (Grand) Susp. daughters (Grand) C) 1 O 0 Note. Animals with tested progeny of Group::z Original reactors appearing in chart one. (That remained positive.) GPDUPZIII Original negatives appearing in chart one. (That remained negative.) Group 2! Original negatives that appeared in chart one that sub- sequently showed slight reactions. GroupZEI Original negatives in chart one that became positive. (Experimental data cont.) Group:iz Possible Inmur e la lilies. Case #FG " honing a family of rosiiive rnimals that In this group I an s liave IN ver etmuvtud. to the particular strain or labor-tic s 115; s |—. A lvidenflfkv or £11 strai519 of {JKUWUJS t been found in the mi in iunity exists 0 .‘ er the? have been exroscd. 5 ll: of all lactating aninals. C e if“ ABI £0 0') his aniza l is negative, she is a full sister to two other ive fezzales number AEE and A011. Cf:3 nega No abortions have occuered in the family of A51 consisting of nine females. No abortions have occurred in the family of AEE consis tirg of three fe ales. Lu1ber nCH had tlree Iorié- l p: rt1ritions and one two lLllfldI‘Cd and thirty-three day non-svecij'ic abortion. (B.coli infection) KEY TO TABLES Symbols used . * Asterisk denotes positive reaction. - Hinus denotes negative reaction. p Letter denotes partial reaction. T Letter denotes trace reaction. The agglutination test was applied. The dilutions used were 1-25 1-50 1-100 1-200 1-400 The turgidity of the antigen used was 7mm according to the Gates nephelometer. All tests were check tested. If any variations occurred changing the status of the animal from negative to positive or positive to negative, the animal was rebled and the sample taaen was tested. Bacteriological technic employed as described by Huddleson. (5) GROUP I Test of entire herd (Arrangement according to age) 10 .5 4. 4.. a .45. :94. .54...“ ..54 I$w~$$ .I‘III“ I. I‘ II II I. unema ----e $#*.=. ..Iobu 4. 4. 4.. II... ...! J4 54 .4 54 . omen uaaaa .>oz some 1.! I4... 54. 4 .4 ‘ -J‘fiuficbf 3 3 3 3 I! I. ' | ' 03...»...3. ‘4 .4 .4 .4.| as. .45II45. ..45. 5. .4 ... 5. 54 ----e "' 4- Oh .X..3. .900 been : .4534 .I I45: '4‘. o .4 c v .4 .4 I. ..o .4 54 3" 4‘ 4. Jfldfi .wn ...... ..n. «0.45 -..fl T...$* 3t..- 5': 5’4 4.. I'”. ".l. u... ....I. ...5. L 4 ..Q sea .m5¢ mdwm -|"- I. wcebvlh... ””0 -54 It 54 "|‘- ““ 1i 4‘ '|“‘ O? r ‘1--- “-“ ---I' now”. I.L-Ouw- 1:. ....a dmd om< mQ< 00¢ QQ< 5 .5 4. 14A. I“: Inc. 3. I54. 54 we. 44...»..«5 .8. IIIBQ l$$$$ n..@@ .11... .wn. sin. *e$** .>oz monk 45. 45. 9; 5a 1450 I45! .541; .4 .4 54 Mrs... .Calfln .... 3 3 3 3 3 ’Q \ U : .54-Woo .wfl Inflow? (a .s I45 1. .w». 54 c.” 3. ....4 I***# 5. I05hlmnllr 5' .0 :14 4 '3? .900 seem -uuda eases ---nu studs. IR f?» “'-| .9 $3". 'u‘l‘-’ ----a .$*## uaaae Ic‘"- slime"... -umsa Itaav l'|" (-4 ..III"* Q*** ‘|-" 33"- -‘|'- Imaae '|“' '3'-‘ (In flung-30!): “"' -"|' II*** ..."..n. .t. .1. .... 5' 5' 8' “‘-* .L 5 5 ..."..Jlla '3--- -II‘II- -"" -.."- ###*# slain...» II‘II" --‘l' -'--' adage 03* II#** "Ielu' O***# IQQ$$ --Rae eases -..“- '3--- ‘3--- -"-' HH¢ mH< mH< MHd an 0H< de ¢H< OH¢ Hmd mmd 0m¢ mm¢ Qm¢ om¢ H0¢ m0¢ 00¢ m0< 90¢ 00¢ m0¢ ¢0< mmd 0m¢ mad Qm¢ 0m¢ mm¢ oz Noam 33333 33333 33333 33333 33333 33333 ...99 33 4.5 45 45 .5o..voul.4| 33333 3. 0 33333 33*» .5 54 4. 5.45 4...: .54. .5. 05% .54 05% 3333* u--IQ ‘ ' ’5 IV» *2. ... 333333 -mmta ItIIQ - ‘ ’h ’0 ‘ I500 '54- ..r .... .... 4545454 39 #2:... .900 seek an--- 3333' 3‘33- 3333' as..# 33333 --*$$ 3-333 33333 oun*$ 33333 33"- 33333 ...:n 33333 33333 33333 33333 asset... I-|QQ nuaea “"- I$$$$ isisisiai» ia¢$$ IIIQQ unmet .wfi< maek 33333 33333 33333 33333 anaas 33333 33333 33333 33333 3333' *ukwxx... 33333 33333 33333 33333 33333 33333 «....x . fizz. c***# Inlmt hash mane -3333 -3333 3‘33- -3333 sauae 3333' 3333- 3-"| 33333 -3333 I$w$$~ -3333 -333- -3--- -3333 3-33- 3.3.. 3333' 3‘33- ’54 I ...... 4 50 .4 5‘4 . l 5 0.. l . ‘ i. earn. .. lxzak |Q*## cash mama Q$#$* IQ$$$ hmz sane 30.3.33 3.33. 33333 33333 33333 333.3 33333 33333 33333 **.x.*# sex...” 3333' 33333 33333 33333 3333“ 33333 33333 Hasa< camw. 33.33- 33333 33333 33333 ##9##.5. test... some: meme 0cm mum mom 00m 0R“ mom dum 00m Hmm mmm 0mm mmm mmm 0mm 00m 4313 DUO, 0mm H¢m mdm 0¢m mdm mam Qdm .04m mew 04m Hom mom mom MQm mom 00m mom VII..- 113 ell-slag“ ""I .nmm nun-lug.”- ofldh. ommk "~H—g* oooQ nmmw ---ee .>oz monk .poo >>¢% .pmmm o¢¢k .m5¢ mdww mafia mon% mafia monk hmz HHLQ4 £09m: peak can» mbmk Haw m Hum mom GROUP I; Original reactors appearing in chart one. (That remained positive) 14 in“ 1.5 oomhp n B auaunun u.n o>apwwon n I o>fiufiua N .3. IIIIQ using latte III## nn### sl### I#### II### tnn## mum Umoa Ing## ##### ##### ##### ##### ##### #####_ ##### mdm I#### ##### ##### Iln## suing llama IIQQ# caaa# ##### ##### ##### ##### «#m |#### nu### u#### Ia### IQ### I#### using :ula# sna## cm### stain usln# mam IIIQ# slant I#### :I### alm## II### ##### alu## III## Ian## IIQ## It### mH< sung: a--- nanna ---:a ua-zu anuag snug: ---ug u-.sn --uaa sung: naunt n44 I#### ##### ##### ##### ##### ##### IQ### It### II### IIIQ# IQ### I#### 4M4 ##### ##### ##### ##### ##### ##### ##### ##### ##### ##### ##### ##### mud IIII# IIQ## III## 33:89 II:I# Ilunt all## It:## II### I*### |I### Ian## m3< Udon ##### t#### ##### ##### 034 IQ### I#### Ina## a#### IIQ## salsa I:IQ# saal# acun# Ianl# Isal# 3391* mad II### ma### ##### ##### ##### a#### ##### ##### ##### Q#### I#### nan## 434 ##### ##### ##### ##### a#### ##### ##### ##### ##### ##### ##### ##### mhd ##### ##### ##### ##### ##### ##### ##### ##### ##### ##### ##### ##### «L4 ##### ##### ##### ##### ##### ##### ##### ##### ##### ##### ##### ;##### oz .poo .paom .m54 hadh mafia hma Henad £09m: omm#. amnwv monk >b¢#_ o###_ maww mon# aon# hoax. oan# a>m# .nmoaodmx A42H3on mmm mam Hmsaoz omaanum a a = «msmua ouanmumn m a g nmnmaub omnoauo oofladx zoo .aam.poz omaomum a a a mundanp Hmspoz mmabaam H a a emanflam woumaoma mangopm.m flagpoz omamaav m g a nmubam Hweaoz bmnaaua n a onoz omuoaao UmpmH0m« maphonm.m .wamopom QNIOHIN w : wk .hwm mumflln “was haw 0mm wcfi>aq omuwmuv n a moa*.pam mmumam auspoz mmumman n a fix .nmm amnmum awaaoz omaanma m : mua%.nwm Hmamflnoa Haeaoz magnum n = oqoz amunaum Hmsaoz omammum m a nu .pwm omnmaun .ompmaoma mapponw.m .aflm.pmm mmawuw n a mu .nmm mmn¢aav afloam a“ couaon< .aflm.pmm omcbfisoa m ocoz wua$ .nwm omamann oHHwo haw com uaom zoo mcfipfig mmuonup w = a omunm-a Hasaoz mmadaoa m = onoz mauoua Haggoz omamaub w = Hm .nmm Hmummuw Huanoz manouo p onoz onoz panoam Hmeaoz bmuwmna 0 wk .Awm wk .amm manbmlb .uaao wc«>«q-.aam.pmm banana . m g vunam.axm Hwanoz L immnmaaoax ,m‘ onoz, m* AmMAIIMHnbmanIIHHr .uxnmaom amsoom coappop4 noapaasuaum .oz upcwowam :ofipnonc no puma .hwm cozampmm mo anon mama mo mama mo .pmam knoumam mama .mmoaoflmx adeUon 5 ’c-n 17 sunnany OF ORIGINAL h?€aCTORS. None of the animals that were reactors at the start of the years test became absolutely negative. A#BAA‘became a partial reactor in October, but became a strong reactor again. This animal was exposed to the pasture abortions 6f #IE and #AIA. Eight animals aborted. Two abortions occured in a pasture with Other animals. -B.abortus was isolated from the other five cases. One aborted before start of test. See diseased membrane charts of cases #AEA and #AGA. GROUP III Original negatives appearing in chart one. (That remained negative) 18 J b .h. ~ ~ ‘ ].9 090m .aan onw§ .ooa amnfi ewoa neon .>Oz momfi .aoo pewe .pnom ovvfi .m5¢ mawa .mm>HB<3mz ease «an» manqommd cash 3% he: rnnfi Huge< oan§ woman Hwapawa o>fipwwma m>upamoa -' --- -"-' "-'- ':-- '--'- '-"' "-" -'-" ""' ‘---' '-"- "g- -"" none: came 'QV . Q ...! ~ ‘ 1“- . Q i. I Q ‘4‘ . I .1 I 1 O. ‘ I .l- . 0 iv ’1 .- . . a... ‘ I‘ a ll . I p o A. u ‘5 I. 1 | I I». 23() mam ham 03m Hnm mum mam Qnm h4m 04m m>m th mad 044 Hnd om< Q5< nhd 0&4 Dad mm4 mad Q04 094 434 had Had 4H 46 4Q .32 omloucb mmlmnnd omIOIHH mulbno mmavam mmanlm mmlwaue wmaolmn Hmeaoz mmummuaa a = = emawauoa vmamao = censuses a = = unease 2 mm“-N-anm .H = .- VN-ON-” = em-sm-s a = = .m-om-n : mmlvalr A = = nmunmno : mmlwtma H = = mmawmcm : mwnndav w = : omInIHH = mmamnn w z = omuomlm s omuamam n g . magnum Hmaaoz amicasb n = = mmlmmue HubOEma Has: E hmwm .wam.pcm bmlolm n oaoz ocoz Hmswmaad 0 “HM. Pwm .meoamonea oeagoup mHOOpmam mmuemum e mnmuaeaem muaaaem amasum Hwanz mundane n = 0:02 Hmsmlm .noapoomna.QoApm .mam.pom owlomlad e : m%.hmm Hmlmlfi .coapoomaa “Hoo.m mdpmm omen omtmmaw w 2 : amlnml¢ : omlvam m a a ONIHHI¢ Hunun henna mnpnofi m 8 m 83 were; ammo Had .ooHHHM : mwlbmnm ¢ : a omcbmlu Hmanz mmlman w = : omlonlm .hoou: and aspen: concomna.noaum .adm.&om mmubnm v z = manoalm .aoood dopoeuaa .n, .nonum,.emflaum Hmsgoz mmamflaoa e ocoze oeoz, manpmnHHILppl mxamaom Hommom Cofipaon¢ :oapHASpawm .oz mpaoomam Qpraon< upon «0 puma .awm cocamumm no mama even no spam mo .pmam anopnfim .mm>HH<5mz mBDAOmm< .-fl. i._._.-.____ n 21 SUMMARY OF ABSOLUTE NEGATIVES. No. CO was slaughtered because she was an aged strep.infected non-breeder. No. IA was slaughtered because of her poor individuality and series of weak calves. No. ADD has a history of severe dystocia at the second parturition resulting in metritris and adhesions of the uterus to the rectum. Streptococcus and B. coli were isolated as indicated in summary of breeding history. GROUP 11 Original negatives that appeared in chart one that subsequently showed slight reactions. 22 1 -.H)~ .4“ 2353 Ilsln ----B --'.n --BQ.‘ .pmm --'-* Islam ----“ -nlnl'a ----IQ taunt satin "IO-* 0 “an 009% align ulna: ---ea I. "BQ O cog nmmfi ----B ....9 all. I'B ----.‘ IIIIB Opoz momfi ""Q --...-B stunt uuuna ----B O p 00 bb¢% IIIIQ align In .paom meek Ilium cl---“ ...!th IIIIQ. "'I. . . --"* lei-I-“ ----“ xxa#* .ms¢ mawfi t..-" cl---“ sash «one. .mJOHBodmr amqum Bozm Biza WM>HB¢UMR '-'-* cash amni has been 9.33.0 on Hague came so.” as meme mum «on on | u Ir ‘5 .0 . . ct ‘ It. \ ‘1! o 4. O I. . I - . l | I lit ..l d. I I II It A II I iv. I . .5 . . . l . .' 0. w it , .| u n u . . n . .. t ‘ 2341 mmsmnma mum mmufiuoa «am mmuwanm on mmuomav u.aoaum wfioOpth mmn¢lmd .;H = H& .awm mmlnloa «m4 = omgoum m = H%.amm nmswuw 0:4 .. omnmaaw n .. = muofiuo mm< Hespoz mmumsn n oeoz a omueflum oH .mflm.pmm canvases e we .aem = mauoflav Hm Hmsaoz methane m = a ofiummuma on O “H“. Pom unease om-emuaa e = 0:02 Hmavmnn on : omummuw m g aw .aem mansum mm a mmsmaufla w mcoz ocoz maamuma an Hmsaoz museum 0 we .aem He .asm mflamauoa on .aHm.pom, rmcmmaa 0 .me .nem Hm .nmm mHuOH-¢ Ha . mumhdfimm Hvamm QOHPAOQR Cowpfihghwm . Oz mvfioomflm Gowuhooa EWM 00 Ho pawn .Adm ooafimuom Mo om span he used mo .amam snapaam .monaoeam amqum some same mm>aH¢emz.qHBdowz O own nmmfi .>oz momfi szH 32¢ m>HaHmom ms: u-nas Opoo bbwfi 'Il'-* .pdem ove¥ IIQ$$ :IQ** .m¢£ maen Qflm B138 mm>HB¢0mz AfizHUHrO hash mama «TEX. * cash mmnfi aux been Hague oana 03m mom cod none: mama mmlmalb . H .uhAOpmam waaeocam com Hmaaom. mmlmmub w Odom. = examaex acumen cowpaon< aoapaaspamm .oz wucmowam aofipaond mo puma .awm voawwpom no open no case no .pmam anopmam .W>HB¢GHZ zmma 324 m>HBHmom MZ£omm BH840mz A¢2HOHmo Quinta 03m vmlbmlb mom 93m .0: open GROUP 3': Original negatives in chart one that beCame positive. 29 53() coma» Hawpama m>apmwoz o>wpamon IRE-4 III#* [*30TX. I**** llama **$#* #*$** *$$$* 0609 II#** ***$# 3.4%.; ***$* Ignaa BQ$$$ I***$ leimlaima. ***** #**$* ##*#* ##$** .pom slams I$$$$ .x. ”ream. 311** 331*# ##$*$ Ilttt «vivaeaela at m* «433% *saaa I I R .x. 2. I9“: *$$$* $44$$ II*** .u .x..x.$* mannivnvam 89.”. a. IQ$$$ as“. $3.. Aa.*** I I*** B$$$$ I**.¥* *¥.¥.¥* .fxvaa. **$** avaenvav¢+ *3: ..llnmt. . own nmm% $$$$$ .I 1:222. w?fl£m.$* ' .l I I II calaaeimae B*$** weavietmae extant aaamt *$*$* iataiataia . > 02 monk sIQ## *aaaa **.¥ 3:8. -a--a .poo sewn "'-' ‘ ---- timbre .x.x:x..x.$ lei-" ' ---- -"-- --"- 'II "' *wxxrfl. «xxx; "" ' $$$$$ *fllu. «:12. I. :0 II I. Q $4390.“. .o\t ..Pn. .C 5P IOsI u: ‘0 - .o ~r w... 0.1%.”; fii.‘ .pdom oven *$$$$. *wxxva. IR$$$ .a.. t#*** 0“. 'I‘.I"O U“. I". .4 .4 .a ‘r .0 “"- .wdd wave 3" |..' A \ I$$$¥ I*.¥$* ‘0."lr‘ \ q *vxxxu. *$*$* hash manfi -..-xvi IQ$$$ I." o\ n\ J‘J‘ I" II- ""' Icn#$ v: n: .a\ n‘ I «v. .ol ludfl¥ -"" 'leHPuT .u .p . -"'- --"- wash mmnfi .mmoaodmz mzdomm Etna wm>HB<3mz A¢2H6Hmo ""' |'-'- "l-' l‘--- ""' hank Haad< Oflnfi noun. mbmfi HH¢ HI ’0 \l O . .o . .1 '4- . e! I .0...‘ .rl..l0.- 3i] .poan uoz omlmalm com .coan uoz- mmunmaa Mom opHpHmom .aama one amuemnH H = = emummuHH mam ohwml 09m mNIHHIQ Gum .awmn mam malonlb 0mm .mel Ohm mam .awml ohm nwtmdlm H¢m .Amml ohm mmuwmlm mHpHmom .awml mam = bmaoano a 2 = udm o>HpHmom .Amml cam stfioz wmlflalm H z a wmlbln Hom .yHeo hue mam - opHuHuom .pamo ohm mnH>HH omomHum H = = emsmum mum o>HpHmom .asmn mam Hmeaoz emuonnH H a = em-mum cam m>Hpamom .Ammu one .mHm.pom omumam H = = emumHum 0H4 .awmnpmom Hmeaoz omaealo H = a nmeHINH nn< .AwMIpmom Had opapHmom .amml cam .aHm.pom omumano .H = z nmlmHlo mad .pmmlpmom = omlmauw m = = nmvmufla mm¢ .ammlpmom = omnnfllm H a a mmlmmlmd and 0 Rd“ '9.” 0m .- mml I0 H : 8 E o>HpHmom .Aaml cam : omnmanaa _ m . = : mleIa HM4 .aJRWumom Hmeaozx omlmama o ofimz, onoz_ mwaonum pm napaop< GOHpoeom Heaven nOHpaon< aOHpHasaamm .oz mpaoomHm noapaond :90m .002 no uo puma amen .nam cechpem ovum aoprHouH ummHo no spam no open Mo .pmam haoumHm .mroaoflux mztumm.8£ma mm>HB¢,m z Q¢EH$HmO 32 SUMMARY OF ORIGINAL NEOATIVES THAT BECAME REACTORS. E1 t animals aborted. Al were classed as reactors before parturition. #303 had two negative phases followed by a strong positive phase. B.abartus was isolated from each case, either from the membranes or fetus or from both. All fetuses were dead excepting No. BOF; a small 252 day living calf 0 See charts of diseased membranes #BOF, #AEI, #BAC. Five animals became positive after a normal parturition. These were all classed as negatives prior to freshening. #FD had a suspects reaction eight days before parturition. The membranes of #FD were absolutely normal. KLOD aborted since the Close of tie test. #BOF that ahorted during test aborted abain since the close of tlfa'test. B.ahortus isolated from both cases. -._..r .1 “w.— ~— T GROUP V11 Record of another herd used to determine or show what nay possibly be the sequel to the post-parturient reactors in group six. 33 c “’3; 4 Form 161—]! BREEDING RECORD WITH REFERENCE TO INFECTIOUS ABORTION ' * Cow NO..M...BCD AGGLUTINATION TEST Isolation of ‘ DATE Blood Milk Colostrum B act. abortus Heat Dates Dates Bred Par. Date REMARKS +eB4— __ Juno - 6/29/2f Sept . ----- Nov. ----- 1925 Jan . ----- liar. *---—- ip/27/25 271 days April ---... Norma]. y ---..- ' July ----- 7/14/2 5 0V . ----- 1926 Jan. Hep..— -(-PO stwparturi :33;t r eaetien) Feb. “15p... Suspect pribr to parturiUion . Bar. 40...... Strono reactor after parturition , { April *1)..-- 4/16/26. 276 days May «ms-M B .abortus i so ated , Normal June W from mi 1k» in lay : ' July mm: 1926 3 7/ 18/ 215 Aug. eta-reex- Se 1; J W ‘ Oct . «was-Ht . NOV . *“H 1 Dec . Mil-H Po siti ve 3 1927 1 Jan . MM Feb. W 4 Mar. *fim Apr. ***** ~ B.abortus isolated 4/21/27 Normal from colostrum in ‘ g parturition. April 1927. : 277 days \‘ Milk ,readriisimnjlln.-. '4’orrey Abbreviations: Par. means parturition or calving. Ixixl 1" illllin ii . '36 Form 161—H BREEDING RECORD WITH REFERENCE To INFECTIOUS ABORTION Cow N0.M---BCE ......... AGGLUTINATION TEST - DATE . 3130:8“33 °f Heat Dates Dates Bred Par. Date REMARKS Blood M111: Colostnun 3" - a ”“3 1924— July 7/8/24 Sept 4 ----- Nov . -- --- 1925 Jan . ----- mar , --..-- 5/29/21 264 days June 6/2 7/2}? fifly’ *p--- (Post-parturient reaction) a%--- Aug. mp- ewe-x- Sept q em- Oc t . «awn-p. «mm... Dec . ate-um: 1926 J an . mus-H- Feb . ***p- 1 Apr“; pp.-- B.abortus isolated 4/3/26; 280 days lay *mp from colostrum * Norma], Juno {nu-p.- ’ 4/3/26 ‘ July «Ra-mea- Aug . *fi-fl-H 8/ 15/ 25 Sept i mau- , Oct . em 1 Nov . *‘fi'fl. Doc . *Mp 1 1927 d Jan . Mpp Feb . «Mp 1 i i J Abbreviations: A" 7 Par. means parturition or calving. Milk records from Mr. lorrey 1926 FIRST GENERATION 1927 1926 FIRST GENERATION 1927 1926 FIRST GENERATION 1997 A—I E63: l March April May J unc July August Scptcmb or October November December January February 111031 M arch April May June July Au gust September October November Dc comb or January February Ilacgd March April May June J11 ly August September OCtObCI‘ November DOCPmlWl' January February i ! 1 26 SECOND GENERATION 19:27 13953 r SECOND GENERATION 9:27 1926 SECOND GENERATION ‘ 1927 __ .3- _ _ l _ l. (323:; A, 1 C25 :_ .385. I 925 ; ' . v v : . T Herd - I I129? M ‘m 11 April M uy J uno July August September October November December January ll‘obruary 111031 March April May J unc July August ! September October November December January February No. March Apr 11 May June July August September October November December January February -—— _ 3 3, ,_ _ A», , . : l -,_. .2 _- u I : l 11“,: BCNQ‘ “1331.3.“ mane-...”: ”macaw 9:; :4 D. a: yaw“? -..-...:w IF- BOO pan-...“ ”WPF- pacer?" 2:"..C—u if I]: BOOI ' E i : Gal? fl 9/15/3n ; a *p--~ : l ~ ~ _ _ : {j (l ’C‘ [’ " :3} W," «gr :1 r l ,,":/1"; y/xg A, i I w ._. —— \ n: L). t" A ’4 :7 [A w I _ y’— 1: not i all, 3-22m i r BOA : t Boa : D- “Lu/l»; 5’98””, l‘ ‘- '. ’ i 3 , , ,, , ‘ I I I l : calvet 5/11/24 ‘ . l x _3 zll333333333-,3im,eo-_ dijg __ i, ”A s l l -3 r _____ .__;.22———H - ,;rfl I l ' ‘1'”: T: : ’— ‘ J“ a“ i : : 1 t: j ‘ . LC? : =: .a :2— -_ 0:: T can 21-. nos-e a l 4.9 “can: pin-ca: an In BO 3:: ‘3‘")("34‘9t'3‘ ] '31"): ': 1'36; 1!. .5221.) 1.11 l ~ L: W " : - ~ e P ., ' van/r35 : l a 3 :8/25/24 :Ersr . rp—-- -0 es 0 V.-] .1 F g : .‘L-‘i-‘CJ' ." ‘1 ," if, < 1 c, "a as t _ :i- _ 1' —— 3 251'; £2“in :— O", /~'3 Jlrfi/lri‘l (if ‘ I 47 dziyd. n n 1.1-1- pallir; 9.1L,“ xillcll 13,343 O I.” DOG r v“ 3 ’ l . — ' I 4 in __:(\ fl ”Isaac-us: mama—swan ; ll 1 5.6.2.. 5... . ‘ In TJOG I l d__) l , | . : 3 l l l ; I lA I I ;A_____,. _A _ --3 l_ — C 7 7 — ——— ————— — —l—V—~H ‘ .‘ v 2 _ """"" ‘ " _' _ Pk.“ -MQ,.. ‘ - “..la- -r—a-n-u- ..--ar- -"°"€-“C~ . ,3: _ w, ; ; valved: ”“3, s ”a” ...”..- P“ BAG "mm" : " g ' C /8/2:" M BAG ---.-:- ........._. ----=- .......__ --."... __________ __ _ _ ~— “"°“"“”; 3”" ‘ : ‘u . "" 3 ,3 _ I 3 "”' "' “6"”- : ‘ :17/‘F2 / 45 : r I 5 CO 0:7? I B14 qd SEE/“Ia 3r 3 l w i ‘25 a Q ’ ~ E 4/10/25 ' 6 6 ’6r3 4/1/2 /15 i ' 7 f. "u :1 3 __ _____ Z)_, <41 <13 _ v‘ —-~—————————-~ I , ...: _ .‘r ‘ lip mud”.- ”1 ll :- ”vars-'2: l. k 1’ :< IE<;_?V‘T"»:‘V ' 1 :_:. , ”mun—7“ ~m--- {IL 211:) ...-“‘F %_*p-- ...--er -~',t“ H : ---—-c- 1‘) an-l..--u. p-” 13-..."— - Gil-a- I“ BAD -~--- ”us-Clo:- p--:- p---~ --a--_ :;p~- .- I-wz-oom eep--~ -.o—u—u-n p--~- ' -~~-~ ‘ ----- l l l l i : 2 fl:}” C-H ijl,’ r' 0311‘de 7/21/65 l /:c (if F pan-.3 C 08/6 (’6 - 'i l J 1 1 ~, ~ /,:7 “IEM 11/30 #34 --3 _ _____ ,, 2423 days ! 10 39/ 53 253; d9 :3 ‘5 _‘ Afii 7 7 ii —f‘ if“ ——’ i~~ __ —— _ A cf * ~ ’ "L: -1. J. _dM———.7—_-.w:"7— ’ ' Y" : qow “Claw.- - a .9 ”-3 1’: ‘-~.g- --w9~- —~~~- I I r L! \t u I w ‘- \, 1’: “- 6 .3.“ i r 3 ., r - ‘ I l 1 "9'6“” 9 - -~ “- l‘t BAP -- ~ -~ " " "" u - E .. n I}; ,/d c -- " ~ ; .2 - .. .. a m BAP ': : ----.. *4‘ "f" ' “PM“? ’I~":"’v~"rrw -z:--::->:-~::-~;c- -:;~::--::-~,:~::: -::~-:z~e.~-x~~::— ee-zceex-ee era-ermi- I l : .27. , 't‘ (3‘- , ‘ > : :. fired o9é“niki 1737905 b.8/lo/éb I : . 3 W: ; ii/b§"az ~' “* v“ ~‘ v 956 da" 1 :;‘_ _, "’"_— ’ff‘: :"i'““]|_'_“""‘_“—i"~"‘——’_-1‘)‘£ff“ —‘;3;? 5; "W "x l ' ...- n l" B“, G. ”-..- - p____- ...:r../: ----sun pun—.- ~----- 13......“ ‘ BIXG -..-“9". - -..-......- p---~ """""""' p-"""° -'-~ix'§ ’X‘pu-m ...—......- ...-...._.. -..-..- __~__ T-c'_u I, ne'er m- » *W ‘ ant-may” .0 - u I -..-...-“ — ,. ' W.“ “A 33:14 ,_-: a L ’ par-av - ’d (,1 31.x K‘d lg/ECH I 535 /Br Cd- C O 1'0/221/26 Br ed | . k::< J: a" ‘,_ . v V p : :_: l2/1U/24 6879 2333/1914fie 276 days 1/28/27 _ ~ _ ___ __ _~____ _ __ g .l" d ‘ ”'"’ ~~W ‘ .n a - .. ..- en C.‘ 7 E F- n- ‘ I. - ’I n . ’ 1 7")."1 F, I ~ __ - u r BIL}: ....- eeeeez_p- e5 O'TI)..- -_.,.._.- —c.."~ ~ l -“h p 4 (11 ’: C-ao-‘un Pl: BA I]: p~-~- esp-l. Np- *p--¢a pp-—- p---— -—~~u. p--a-a- --—-- p-~-- p-~u- J p-~C‘~ ,‘ ~,_‘,_ an r-aHI-s a: fi.. m- .g.......,...,.. ...-r- :. ‘7. .0. I r: _ ”/1 J- v' r t ‘I l 3 2/17 as 273 , V’s eqifyhe 4/24/2« /2/25 “— ‘F: .l: CK ' ’ L '— c-r .— uu- T’ T v 7:” E,E ’ uh-... I -:_5u__'_- m; “Uh“ ”a”_- ..-..-” ,3 BBQ ..---.. ‘X'p-"~ poo-awe ____,=__ ; -—~-—S . lfl _BBO -—--- p.--- ' ' /_ / f ._ - ’ 'J . “U W - 'l W I x.: ,'/\‘ _, A b~ ‘\d an-..“ a I l l “ ‘ " :9: 25* Z/Q/zé p : (C, 1: Ie/Es as? any i “- : {Y n; r Y} 53 I I: . \r u_ v , v u ' x v' “...". .‘Lé’. .‘k.".."«.". °‘~ 0‘ v - ‘~' -'- ~ ’ ' ' ' r I \r \I \I I I ' ’ V V I 'H I \v u , l N 727:“: Y " ' " “ f C ll Cd— : ':: OM «' ”"2“? '1’-""--2{~-2"'.. 36:754.“ It T‘D A .v v v ., ~"~»‘-¥¢'~~2’»2I~ €l~2€1£4~€£~ 6169365951" '3"" "‘13”: it, 9” --,.. ecszfif in: h 1:” n n n I: I: I: I: ‘ h-x idea-:13... M BEA fink—hem 7:02:6er iv): in?» ic'umvrw €1.72 6:66—23 6. :ee-x-ee *2 ' “X 95::- 7:66: -2(-'.‘C- étdtéi‘étp ewes-re.- v‘i‘i’n‘fi-fl'p ‘3‘ Ht?!) I . lg.)¢_ 4;"; as - — on no "5‘ "n‘ ’n '1.’;.‘ a_ I -~‘ ’5; '4. I. ', ‘1 7 'r. 1‘. n \.-e i 4| '. . ‘- \ ‘ iD‘AJn “- '1: v, -', ', n t. n » ,~‘ ‘ A D 7/;U/24 bred .;;..;;.-;..;:,-:;- eea-xp- ‘ delved lO r7/25 prod C .11/1 /26 « ~ ~ r 211 drvq /7/95 __ 27$ d“u ’119/26 q; day C~ _._-____*___. ‘ _~ _ c, 4,.” “m_“~_ _l___ -... _ 7‘ Dy: ' ‘ ...-.5... -—._:'__.’ -H_____ -—-*-.- --.-.0- ...-I." [V1 BBB “---- --m—- -..-c.5- -—~¢—e- --—-— -..—-.. -—--- ----nu -———— ----- --.-c.- ..p-‘ 1:. 4.2L] —-w"~’~ “'""" ----.. M. BBB ”'""" p 1|” B ma ‘1 .: ~ Bred be: 1 VM /1 7/20 ,I‘: 3 cl . 10/19/26 are? u 4 /r, 261 d‘;‘t’3 1/15/23 9'70 dawn-a ‘l /'n 9" H — ¥»‘_“*--“ 1 do! ’4 u v I - V J' 1 ...-men: $3257.")? 'x";\‘7\‘-- 'X’ ’33'zi'n-u Y" .9’ V o" J’ I . "’ I t. ., V3.1 H I B D}‘ 1 ”spam,” 'i'vp—c-c- 6361's.“? ’25 BE BBG ‘2:"::":C';:"::7 -)(‘-,r-,:-}:p 'I‘E'Jni'um- ’;i‘mum “”“”'- :7" ”VP... ...—F-- p x p 1 his: ..le 1“---" fifp-n- ”p..-“ “.-"'-"‘ ficp—u- *p-FF *p-u-n ’X‘X‘p—o— 94—0.“- "x‘*——~ ‘3? 0-..- T--~ A. 4..) h) t ,_ ‘ .\I ‘L i h a; \I \l \{ .‘l‘J'..". If. yam-'0'! %p~-u L’23.l\]t.301/5/26 p :d r l i-lw-arl “‘P‘“ £9er ’"M‘r- “ " """’ 2a6 da TEE} C .1 9 '7 ’.L ‘ _ - \ ./iv/24 (0. 1/12/24 270 days) 4/22/25 “ YB h, 4:6: l Brod Ualved 1/8/25 4/30/24 263 davs l u [’1 RBI fi_.—_ '22- 47,1- HI, __,_. _ . —~2 _ 47 7.. . »-_,_, , W’W—v—vw— 4—_fi-w._— Wfiw", 1926 FIRST GENERATION 1927 1926 FIRST GENERATION 1927 1925 FIRST GENERATION , 1927 112 31‘ M: mh ~ April May June July August September October November December January February 1:951 March April May June July August September October November December January February { I1I\Te(1)d March April May June July August September October I November December I January ] February I l l \ l _ I l l l SECOND GENERATION 1395?8 I825 SECOND GENERATION 19526 1926 SECOND GENERATION 1927 __ __ _ ”A" 3 ___, II _ r- - .... 4.4, ‘ fi . 11%;} M 1r: l1 \pi il l\lay AuguSt ] September October November December January February 1%)? March April May J 11110 July August September October November December January February Iigd March April l May June July August September October I November December January February M BC ''''' ..--...:- """"'"" M BCD 4*. .4..- .......... -..--- ..... _____ **P-~ **p_- M BOD *---- *p~-': *‘397‘3'55’33‘ 5543993393 '35'33'35'X‘93‘ *fiHi‘éé'X‘ 46444-434:- -e:~4:~%-:(- l 434544-341) eageggaep 94.344444.” %.**** C .3/2'7/25 Bred C 04/13 596 I - M BC ‘ M6 ~ --— '71 day 7/14/25 : 276 day s l 4 -..-..- --.._~_.- ’ ~~~~~ M BCE 13"“- ----- p“-..- ..-—..- : ---------- p”-..- *p--- p“"“ Inna-cuc- M BCE ..--..- pu-‘- %:-~-- 9%-..-“ pp-..” p-~-- p'”"" -..-"'I' I -‘-~ *cn-I-I- Tau-- T---- 0.3/29/25 4a---- p---.. C./1/3/21? Bred p p e L e % x 267 days Bred 7/7/25 «_ j ' 570 day 6/19/26 1 :m BCF -..-..- _ a .- c. - *p--- 615-393(1)... I ‘X‘éi‘éfi'fiu *%%p- 'X'iépn - "k'x'JC‘x 'X’ '5? V y X * *‘**p :M BCF **--n p..-- *‘%**p **p~- T’IJII’V"), I“. J a I 2% we), M“); ;’ 'MT’IAH’V .3! “I.” * ! **%* I ' n-ua- . uuuuu -..-.-- »~ 1 ~ ' " " " 1" "l‘ I K” (fun; \"A I: - - *** y‘gu Bred. l 1 ' i 0 ,5/29/25 Bred 4444...... 4:-::-4:—4<-4:- 5 4:44:44- 1 C ,4/5/2 Bred p pp ‘ ”*‘K'P 37/8/24 L j r ' 2964 days: 6/27/25 ’ M BCH 280 day 8/16/26 I , I ; étaléteH‘. __--- l ——————————————— -..-..“ -..--.., -....- ____~ _____ I y l .......-.. l l «6'» M BCH ...-u. : ----- ---------- ----- ----- ...-...... 4‘ ‘ p I --~-- ----- -..-..- ..---- l : 31’ ed 1 ‘ i l 0 .5/1/26 Bred L 35/11/86 Bred i g&%flm l _: l . ; _mflrflwwm_Hflmfifiggggwwgfle &qh@5.:: m1: 9M ' 4 p---- _\ .~§.. ..‘0. -§... Erna / _b 91.14; 4 ENY 1926 FIRST GENERATION 1927 1926 FIRST GENERATION 1927 INOil \ March April May June July August September October November December January February I liegd Mal‘Ch April May June July August September October November 13000111130? January FObl‘UflTy . . 1926 SECOND GENERATION «)7 1926 SECOND GENERATION 1927' __4___ 1__ 6 192.5 15226 I??? M arch April 1\ I :1y June July August September October November December January February Iiféd March April May J une July August September October November Dee ember January February Bred ‘ ' 11/26/25 Bred C '1/10/2'7 l4/2/25 258 days 4/4/26 * T I p-D’” -9agfi ...--..- calved -u-‘m- ..---- M BF‘) .0...” --"""' -------------------- ----- --.--- ..... (‘1 1:7:-/26- - T-~=- Bred ( Doe-Ire: 12/18/25 red ‘Eéeédfol 4/1/25 l, a__ 4 A r 271 day B 3/19/25 ‘ “3’5 l c r , w ‘ ..--neo- ‘ 9 -g~-~ : 1 ..... _~__- _____ ..--..- M BFI) ...-~ --~-- -_--- ----- -"""‘-" -"‘"-- --------------- ‘ -—--- ----- f" 1 | . l . a i 4/18/65i r l 7 I 2.6164; s 4/10/26 ; -..- _______ --..-- _____ B t “““““ i 1 a“"‘"" i t ~~~~~~~~~~ -—o-7- ~-~-- M BFF' -__--' _____ B d -ncz-c- --~-- “---- : n-un— ...-..v—o- -— I’05 i ‘g C . 1 20/25 Bred re 3 4/137 Bel l l i 1 l 272 days l4/29/26 5/28/26 1 t i -.."-.. ‘ i ..... L T ...ew...’ E p---‘ ' Colt-CI- NI B4 -"Fo-u- -..-r-— .~---- -..-~- -~—-—- ~---— -~——- ---------- --"’- ----.. ---“~ ‘1 l i I Bred ‘ C .6/6/26 Bred l ‘1 l i l9/18/25 261 days: 9/10/25 \ i I I l ‘ l i _ _i'____ ‘_.____________‘____—————4 GROUP Vii—1' Breeding records of group one with refer- ence to the agglutination tist of the dam and her progeny. Breeding records accounting for the females without tested progeny in the herd. 4O T— SUB-GROUP 1 OF GROUP "1T1? POSITIVE AlfII-IALS KITEOUT TESTED I‘ILC'GEI'Y. 41:3 POSILIVE ANIMALS WITHOUT TESTED PHQGENY. (Sub-Group l of Group V111.) Calves Bate Total Living That Aborted Untested No. Born Par. Malesmflgied‘qgalxes Egmalq§_ufigmiggg_ ‘__ FD 5530-18 6 ‘T' 5 2 F7 O 1 Post-Par. reactor. (Group 6) EH 5-1-19 4 2 1 F 1 o Per.#1 History of (Group 2 See footnote) abortion. GE 4-28-21 4 1 2 F 1 o Par.#l 192 day (Group 2) abortion. II 4-4-22 5 o 1 M 2 o Par.#2 end 5. 81 and (Group 2) 148 day abortions. HD 1-20-17 10 2 2 F 5 0 Chronic aborter. (Group 2 See footnote) (offspring AFO sold) ABH 5-15-20 5 3 O l 1 (Group 2) ACG 5-15-21 3 l 1 F O 1 Normal 280 day (Group 2) parturition. Calf died at 7 mo. of age. AED 2-2-22 4 2 o 1 1 Par. 4 1 221 day (Group 2) abortion. AGA 2-7-25 2 1 O l 0 (Group 2) AGE 6—15-23 1 o o 1 0 Par. #1 256 day (Group 6) abortion. AHG 7-16-25 2 1 1 F O O 284 day parturition. (Group 2) Calf died at 2 mo. Of 866 0 A10 1 O O l" 0 Par. f l 201 day (Group 6) abortion. BOI 5-7-24 1 O O 1 0 Par. # l 230 day liv- (Group 6) ing calf. Killed. BAA (Group 2) TOTALS 14 animals 46 16 10 15 4 #HD One animal was disposed of.(301d) Note. #FH died at start of test. #HD sold into nearby herd so record was kept but not recorded on test of herd, Group one and two. F Female M In “9.1 O SUB-GHOUP 2 CF GROUP V111 IEEGJEIVZ AI~T'"11.=.LS ‘.'!ITEiCUT $132131) mommy. NEGATIVE ANIMALS WITHOUT TESTED ROGENY. (Sub-Group 2 of Group V111. . Calves Date Total Living That Aborted Untested No. Born Par. Males Died Calves Females Remarks Jfi ‘33—‘16218410 4 1 5 F 1 ’ 0 Par. #1”§ead 2 del. (Group 4) Par. #2 1wins died. IA 1-27-20 4 o 4(2M) . o o Calves died at 2 to (Group 3) . (2F) 4 mo. of age. GA 8-50-20 4 5 1 F O O Pneumonia. (Group 5) AGO 4-20-28 4 2 1 F 0= 1 261 day parturition. (Group S) Died at 1 mo. of age ACH 4-23-21 4 2 1 F 1 O 255 day abortion. (Group 5) Strep infection. ADC 8-2-21 2 1 1 F O O Calf died from (Group 5) Pneumonia. ADD 8-7-21 4 O 1 F 4 O Hist. of severe (Group 5) dystocia. Uterine adhesions. AEB 4-22-22 3 o 2(1M) o 1 Calves died 2 to 5 (Group 5) (1F) mo. after birth. Pneumonia. AFC 8-20-20 5 4 1 F o 0 Par. #1 Calf died at (Group 3) three mo. of age. AFD 11-3-20 4 2 2 M O 0 Par. #1 ani 2 Calves (Group 5) died at 2 to 4 mo. of age. AGI 1 O 0 O 1 (Group 5) AIB 5-12-24 1 l O O 0 (Group 3) AIF 10-14-24 (Group 3) 302 10-14-24 1 o o o 1 (Group 5) TOTALS 14 animals 46 16 17 6 4 F Female M NM1e SUB-GROUP 3 OF GROUP VITI' (Family Records of the Dams With “Tested Progeny from Group 11, Group 111, Group 'TV and Group VT'with Reference to the ‘Kgglutination_Test of the Dam and Her Progeny.) 4' S Summary of Brpediné To ta 1 £23311; Ere—fl j. n (IXZSIETKFC.DaLY .4111; (‘1‘, +~‘.L ”a: /: 4-47.37»... ', \ :1 HH- “:1 1 111.1111. .11) 17(- a]: -=. .L V ._L . r71 " 4’? i” Records with 11913911521105 to 44.5511113111125121011 ‘1‘»; 7. S eC 0 :’1 d G 5:11. 3“ <3 T’ri‘c". 1 t3?“ _ Note. #GO sold into nearby herd. The aniual always tested positive. Test not recorded on charts of L*roup one and two. ...—£3.” fig! Normal Po 8 . of Deceased ‘ - - fit0 (I W W w Dam Par. Females Femalggm, ._ébOTPiER§11§§§;_ ROS. 22§LQW.F§E§ELM,N”‘ T08 MPEP§£LamBE§Iu__ —AI 8 ”"W O -WHT—T 4 l l l . O O O O 0 5 O O O 5 ,0 fiAEA C O O O O O 1 O 0 l O #803 O O O O O O O 5 O O O 4 O O l O O O O 0 DB 0 o O O O O O #300 O O O O 0 DD 5 O O 5 1 O _l O O O O O O 1 o o o 1 o #BOF o o o o o 0 El 7 o 1 5 O 1 2” O O 1 O O O 4 l O O 8 .O #1193} O O “B O O O 1 o O 1 0 #A10 ,0 O O O O O O O o O O O O #BAE O O O O O 0 FG 4 O O l O O 5 O O O O O O 1 1 O O O O ,,.5AFG O O O O O O 1 O O 1 O 0 #21111 O o O Q 0 O o O O O O O #332 O O O O O 0 Go 0 O 2 1 0 J2 O O O O O 0 (See 1 O O O 0 #1311119 0 O O O O 0 foot- 0 O O O O O #BAB O O O O O 0 note,) HF 5 O O 2 O l 1 O 1 O O O O 4 O 5 l 0 -#IC (0 O O O O O C 1 O O 1 O O WARD O fr‘B O O O. 0 IO 4 O O l o o ,,5 o. o o o o o 1 O O O l O fAGG O O O O O O O O O O O C) #BOE C) O O O O O o o o o o o #3130 o o o o o 0 7—424- 5 0 l 5 1 o o o o o -0. _ __ ‘ 1 0 1 o #AHO o o o o o 0 TOTALS 9 5 4 15 1 of the Dam and Her Progeny , .- \ 1 T111. rake-13.6 m 1128 u [GO—ll BREEDING RECORD #AI with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION Date lorn FIRST GENERATION April May June July September November February 1 l .10/27/16 4*p-- **p—— **p-- **p-- *%*p_ *%*p. ***_. ***pT sncoxr)ouxuuATMr< 19m; SECONI)GENERATKX€ 1:4)Kllijlnhinli’flir \‘o I Date Born \llhlys in Utero Sex Remarks April May June July September November February [ ,A_______ _ ._ _—__.____.4 ,,,,,,, A 1. __ _,_1 AI l l ._-11_ E12/20/12 E 272 1 Male Died ! ' j I AI 1 1 ; I K ( 2 (10/24/19 L 211 Female "ecfl_@_del. 1 l 1 .41 l g E i l ‘ E 5, :1/2/21 L 277 Male Normal ' l ! AI i l 1 3 z .4 43,37.;4:' ’.3€’7:€$ J'ecee ‘e eeé'eeeeee 4:749.=.». eeeeeeoca. e: 4e».447:4e ‘ 4 ’ 2/ 7/ ‘ 2 l__ 286 y I? emale 1116131112111 _ " "' '1‘". " m " . * ' T 1 AI 3 i 5 24/15/25 '276 Male Nornal __1l_ 1" AI ‘: i i / / F : ---—a- --"-" ----- ----- “~-~- -'.-"'~ ----- 64 37127 24 271 .emelew__ Tm‘lr. or —— Bl ' ' a gglngand .1 eformed 7 10/10/25 269 bemale gilled. AI 8 10/17/26 266 Male Normal \ [ lo Generat on AEA 1 ¢ ( s _ 1 . 5/22/24 266 Male Dead @ del AEA 3 2 4/10/25 259 Male Dead 6 de14 ARA 6 ; 4/24/26 1256 Male Normal K11~ vBOB 1 7/18/26 275 Male Normal Sold ' . breviation : Par. means parturition. a. m 160—11 BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1927 i Date. Born FIRST GENERA’l‘lON 139:1 March April May June July August September October November December January February 1 u ! fix/1.].//1LE) 1)I3 eeeeaeee.. eeaeeéaeae 424.4.9444 eeeeeéaelp seareeesez 434:425243 424442437 aesceee... 449.7.4ree 4:4eecee». 444444631) geeee:ecec a SIC(‘(1)ND ClCNIRA’l‘lON 1920 SECOND GENERATION 1927 '\“ 'f‘ u‘ I - 7 ‘ ' .0\:,H{3(1)>1”1f;.8_f \v I Date Born Days in Utero Sire Sex Remarks I‘lfigd March Aprll May J unc July August September October Novel'nber December January February ' 1 . . r . . H. ! ‘ J. . 1' _7__6__711_ l DB : l 1 . i i 1 l 1 - r l , l .1 1.1 32/14/2 1 ? 1.62 7; 1... i 1:4 2 1 l 1 . 2 ‘ 1 I i l T ; !_ i L 2 17/11/22 3 292 g gFemale (Dead 0 del. ‘ l ‘ ‘ i l 7 ' ' A i“ I F 1 1 ' “ :28 E a ; 3 l 4 r l i g E ; lfibrin i7 ' fl 1 l 3 9/21/‘5 ‘ 263 Femalesgflezd @ del. L 12B ; g s 1 i, 1 I i 4 9/9/24 262 l ‘Femf‘ 1° 1 Duo 4‘2 del i | L , ' b t N I_111 111_1_11:;: E l_1:;;11;;1¢; _1‘ a, _ _ 1 DB ‘- i " e 5 1 l i g 7 . L 5 9/5/25 Female BCO p NO r‘na l i .__-.. .__...“ lo breviation : Par. means parturition. imummn . . - BREEDHK3RECORD #DD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1927 l l w r 1 1 r 1 r 1 T 11-0111 , . ' Date Born 1‘ IRS l GEN luRA ll( )N No March April May J une July August September October November December January February 5- 4 l i i 4 7/23 y/lg DD Mex-4:4- .;4 47444.34 44., .;-;4-;4 ewe-reap 44-44-1449.- 4;~~..«:. .4 44-44-72. -;:- a—-.:~-,.»;;»;e 4-444. 44- -::~:4~-;:~;:~“~ +4444 '.. 4444-44-13... \ '. l SECOND GliNlClLVl‘lON 1926 SECOND GENIERA'I‘ION 1027 . . l "M " ‘ Oils )ruw ol I 1 4 . - i 4. 4 Herd - 4 v 1 . ,1 o). _ \. Dale Born 1,333. m {WWW gm. Sex Remarks . March April May June July August September October November December January February ()‘v‘v :\(). 1(11- .1“). ‘l h()- ‘ ~ i3; ”‘1“ H “TM * I ————- —7 1 __ 7 . i , . l 4 1 , » v | i f l i E g I,” i ‘ "'" I I1 I ____ 11 410/10,21 i WTHMale 4 V4 loo 11 DD E i l 3 i l i g l ‘ I 7 g / / i : 3C i l 2 ‘ 7 4 29 I 19" ‘ ‘ _- " N psi .1 _ -l -‘ ~._1_,, 1 1 _ _, _ DD 1 l i , 4 i i ‘ ‘ l . 4 l I . 1 i ( l l 1 i _ ; ! ‘ Y "7 : ' l ,' x ‘4 . 1 5 9/7/25 279 a (Male , Normal Sold i . t L 4 —* 1 ; DD l 1 1 l l 7 ’ , I ‘ i 1 1 ’ ‘ l \I O I Q! I \ Q | R \' \' \I I \ £1 \ \I \' \ 5] " l , ‘ ‘ l . .. c. .. a. ‘i -- --- .. — .. -- *‘K-c-u- 46765.65- “int-'X'p- ‘>\"n"t\“u"n‘ is". firm-w em. a. 7.". water/rm *1rzr'2r2. 1‘4"). '2. *5? l l 1 . ,.- ( I ‘ r , 4 ' 1 4 .a/o/24 . 274 i _ F.16164_Nermelmwflli BOP _4, or , i 3 l t f i ’ t 4 10/27/25 ’(‘1‘ 53. ...: (D U 1.1. Q i l i r I i % 16/11/25 1 277 i i I 4 Third Gene‘ation ' BOF In—J 9/15/26 252 Male 8 no. Calf f i ‘ bbreviation: . Par. means parturition. . 1 160—11 BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY #EI 1926 FIRST GENERATION 1927 Date Born FIRST GENERATION 112931 March | April May J une July August September October November December January February 1 i i l i 1 2/6/17 EI *%***_ f *%*%% *%%%* ***-- *%**% %%%** %%%%% Killed : I 1 smox I) c: ICN 1:14.11103‘ 1926 SECOND GENERATION 1927 mi) f'\f:\'(l))1'in{€l11f V” 11 Dar" e Born [Days in L'tero Sire Sex Remarks T1931 March April May J uue - July August September October November December January February El l 1 l 1 l 1 1’ .___1;,1. 9/5/19 1 1 luale l 1 El 1 i 1 1 l i , 1 , l l l 5 1 I 1 1 a 1 :‘ ,_ 1 » 4 <__ l V ‘ 1__272 1 i‘enale‘ Uiad _ w 1 __ ~1_7 l l 1 1 | I. I I 1 l 1 1 4 1 4 pp-x *p 1 94»? >4--::--:& -3(-~):--;(--;& o; ;- aese-x-aeee -x-o;:—-;e-;;«;;- ‘ was- .. : Meet-me emcee... .. *ez-eeme me 62-11-36 +4". 51-21;:— -:4 m:- 4-2:- 5 {10/18/21 1 292 1 lbemele Normal g ADG __ f l '. l I 1 1 F El 1 1 1 1 . I l 1 1 l l 1' ('x I, - p, i : l 4 11/4412o 282 l 1__1lMale.___,Killed_a -_ 1 _El . 1 l --°‘-M ! ..-...“- -----— --~~~ --u-c- --—-—-—-— --.-.__.- ..c-so-c— -———- ...-an...- -—---- ...-CD- 1 l .5 1 44/20/2341 2179 1 Female ‘ Normal , AIO , , 1 l i i _ - -- '- ‘I- l .. .. .. ..‘.. ... _______________ ... — - -_- - — - -. - - **':\‘- .. ‘X‘fiHHH'r 'X'ér'fi'ié‘it “ii-ii'fi‘fi')? 3H??? ('fi‘ 151 '2/28/25 281 .1 Female ,_normalmnm, BAE 111,4 ‘ - 7 63/6/25 285 Man re vealed Third Ueneratinn 1 1/27/24 240 k"erriale Dead @ del ape O 1 i 2 1 8/50/24 156 l Femal 6 Dead @ del . .ADG *f -8[4r/25 . 281 Female Normal BBD I #403 DG Ifi 12/1/26 Female Normal AIO .1 9/27/26 277 Male Normal Abbreviation : Par. means parturition. 160~Il . BREEDING RECORD #FG with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 19‘)" Date Born FIRST G ENERATI ON lg? i\ larch April May June July August September October November December January February 1__1_ 11.1.1 -11 _1_ 5/26/19 - PG 1111* Killed /6/26 uh» b SECOND (llCNlCRA’l‘lON 1926 SECOND GENERATION 1927 ()l‘l‘sprim: of 1 _ 4 4 . n 11 4 Herd - M 1 v 1 , 1 1, 0w V11 V11. \*0 Dale >01'11 Days In IMero Nre Sex Remarks No 1\~Iarch April 33’ June July August September October i\ovember December January February 4. - ll . I . 1. .A I I . 1 1 ~ 1.1111_1_.__1 ~ I 1pc 1 1 1 I 3 1 4 1 (r I" 1 l ‘T - , 1 ("7? 19 p 1 l\.-n1er1:2'l Up; ed . f 1 w ‘- 1 ‘ v Wu v ‘1.)- 1’ w"; JV r i l 1 i 1 t I i l l ‘ l I I I l p I \I \l s) \P ‘1 \l \c I ‘1 H \ I \I \ \I \ \I I_ I Q a \I s \ \P u 1 a \I ‘,- i I l I ...-Q... l --_-"' ”---- *fi -- M?e\'7\"¢\- 'lf'n' "A”A‘ ')\"r"7\ '4 'fl T€.h°n'n ’n‘ 70";- *7. ’n '1'.“ 'l '0 'A ‘){’2- .h": .I\_ (‘0 «:1 5.45; 1“"1 Female iflogmglwu_ 1 AFG 1 1 | 5 2'17? 1 Farm 13 . 1 AIH ‘ 1 l : 1 1 mg l l 1 $ t 1 l 1 1 1 l 1 I 1 1 ‘ ‘ u . \ I l I l A \n \I.\I_.\I 1“ "\LIL r_ ‘1. I.u_\ \- I1 4 2 1 ‘ 1 1 ‘ 1 ...-CD *p--- *FHF*- **%’X ‘:\ I *‘X"? '2: '; l 'X"::':~":f';§ '3"; Tu '0'" 'n“: 7"!\ n 1 '3‘? l\'l\)ls 'fl 9"“? '1‘ . l _11, 1 LC: 1 PA emalc 1 1 1 1 1 1 1 y 1 _— —— 7’7 ‘ 4‘ — __ . / 11. T 1 . 1 . I l 1 l 1 1 l 1 1 1 _ 1 1 Third _ ~ 7211 1 11 1 ‘AFG 1 1 benel tion1 1“_111 1 1...: D3 "\ 1...: TV) 35‘ U3 N 133 4: 1b.. 13 .44 V 1m (3. Ngrmal J ¢g1w~u '1 . 1 1 43/1/27 Female; 110mm] 1 _.1_.1__.._ -1 __ _..~_._._-.1 4..-,.__._____.... " bbreviation: . Par. means parturition. A USO—ll BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 . FIRST GENERATION 1927 1311111 Born WRIT GICNICRA’I‘I()N 112311 March April May June July August September October November December January February _V _-,_ 4_____V .4 ' I I \I \ \ I \l‘ \I‘ 1'" I‘ \'_ I_ I I. u“ \1.\~ L\I_'\l_ "k'ii'k ‘33}: *7 I ’l ‘3‘ "n 'n )l\':\'l\ ‘$\'n“3\ '1‘ 'n .I\.l\;l\'l| u L ,. QI. \l. \t_ «I, I_ .v_ t'. H 1‘ \(_ \ \'_ \I I_ \I. \I. \_..\1 l_ \.1 \I‘)' a! is '3‘ ‘nfis'n ‘1‘;l\'% 'n 'r. 6"n'n ’n 'n "x'n '1‘ '1‘ w T‘." 7‘ " rill-led SIC(,‘();\'J) (.HCNlCli:\'l‘I().\' 1926 SECOND GENERATION 1927 . . T -qu v --- 1 - 1 _ . , , ,1. 1 * 1r - 7 r e , w . 1:)l\'-:}1)11“1“), :1” \v 1 1 Date torn Days 111 [Hero Mre Sex Remarks Igod Mareh Aprll May June July August September October l\ovember Deeember January 11 ebrualy L . , Z . 4' l . ‘ A . 11 _,_ 1 GO 1 a 1 1 1 1 1 j 1 1 ' 1 m 1/ A a l T 3 3 l IRE/E5122 1 lee 1 1 IQ mean Q del. GO 1 2 1 . 1 1 1 1 1 -. 1 ‘ :- 1‘ 1 I 1 ‘ 'T ’. I' .f, ‘- r A 2 12/3/25 1 258 1 1 kale Normal _hil} d 1 _‘ 1 T 1 1' 1“” t ” "" ' . " r1. ' 1 1 1 1 £40 1 1 5 1 1‘ J A "LJ"U"’,""" A r «I , u r u I n e \- I r I u I u u I u I u r , e w I U u / '1 1 ; 1 " " " " " 1 %o}(~~)(-'I'-31 36-31616 , 61-61 ‘33.)?“ ewe-a4:- icéc'IHr» *éréficw '3? '%sn: fi-énr’kw '3Hric'3rk *7('-.:'k‘%. fiver-1.1."): 1 I 1/E/en 1 271 , female normal AHF I g} (13 (:3 _1 -.st'y‘ u_.)l. I , , .. . . I , o l ., . , I I . . ‘- . A 1 n 4. 1 n 4"- -)‘ ‘Xa. -;;- *‘X‘e. 93% 'X"):%» ‘3.“‘3: ire: 'X' it"): 'X‘w'ic'k'ln 'A‘flcer'X‘m' 71"I\"3:'3\"3~" '3("3(“}\"X"X‘ Slaub'l‘gt 631" e d 5’) fr (‘1 F 1 {A 1 "(I 1 ,._ .1 1 4 _la/a5/afl 48; female Reree‘ BAB {‘3 _.___—J C) ..- I -.E A ____,___-.,_1,__ 1 ‘. ‘ ' " ' ‘ “ ‘ ‘ '1“ ‘"““”‘" Vow had net; ned ‘ fetal pl? 0 a . hale Normal K11 ed OT 1‘ L—v ——--—— _—.—A_ Adv—a—A _— u 1 1 110/27/25 ”84 | | l 1 1 | ._e . -_——r—~—-— —- Shire-1 -» GGH “f. at: _ —~—-e m“ __, AHF l j5j31/26 269 Female Normal __..-.__ .Aq-WM~-_ .. 4-”, _ .__—.— ..1PA bbreviation : Par. means parturition. mumwu I #HF BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1927 \ Date Born FIRST GENERATION 1 £931 M arch April M ay June July August September October November December J anuary February I i l ? 1/6/18 HF *p-—- **pp- *%%*- **p_— *--~- **p-- **p-- **--~ **p-- **p—~ %*—-- pT--- ‘ 1 ,% SIX ‘ON I) (,1 EN JC l’qu‘ION 1926 SECOND GENERATION 1027 0\:,)&:1))1mf,f:l No. \ Dui e Born ilk.“ in Utero Eli re Sex Remarks 1?ng March April M {Ly J une July Au gust September October November If)eeember January February I HE E l i i 2 ‘3 1| ; --.-g- -=--- --—o-— --—-- ----‘- --“‘-." -"'"'~- ---"'- ..--..." ----- -'-~-- T---- l L 2/15/20 i l iFemale l Normal 10 HF i '1 i z , l l l i i 2 ; 4/9/21 a are i fear; fierwel J in 1 T ! I 1 HF i 1 a : . g l, l l 1 ,— ': I / ,_ l ‘: i . , i i Q : Bylaygz ; 4281 ; {Male l Normal ._- j l EF 1 = . 1 T 1 . l 4 12/181" 25 i 2‘75 _,,_W_gif‘_e_m§3;1.§w_ Norma-ng, ,, % AHD ‘_ e A ' l v = * I l; i E ll 5 --.-.-— --v-o-— can-on-- -..-n-.- -xt-‘- ----- ——---— ————— *p-u- ; u . 10/1125 ' 289 . {Female Normal BOA l Third Generat’on IO 1 1 6/15/22 281 Female Normal A00 Deed H 10 2 12/12/23 292 female Normal dAHC Died Positivenanimel 10 I l l E l 3/3/2.w 298 Male Normal _mwr IG 1 l g 2/2fi/27 ’ Female Normal Died later. breviation : Par. means parturition. I,(")()~II BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY ( #IO _, 1926 FIRST GENERATION 1927 ____ _ — _ - ml Herd - , i Date PM.“ \ FIRST (‘ ENE RA "ll()\ No March Aprll May June July August September October November December January Februarv v [ -_I ’ l l I l/‘r’ 6/1; l 10 *%4*>t'><'p Hex-ma- mews: '2: *%—X—-):%: ewes-x :- Dead l _,__- _\_ Sl-I(‘( )T\'I) (HCNICR.\'1‘I().\' 1926 SECOND GENERATION 1927 I _ \:)l\ (1)” ”‘1‘: (:1 No I Date Horn 111m \ in Ute -ro Sire Sex ’lemarks 13:31 Mare 11 April May June July August September October November December January February 3 _, l _4_- __e____ l -_ I _- 7*“ I A I _, “W b “H“ _ 1 I > a 1 l l i i i 1 l. l i. / l‘ ' Tl VM i 1 ; lit/15.5 '22 i 282 i lma i “0'" 81 l IO l : . i 1 l * 6394-73157 . ., _ r , ; ‘ g ”9/ A fl r 0" I I: 31.1 .‘ ,‘ *‘33‘X“X~X- $3 'B‘ét-Z‘c-Z: %7\-').~1c7: K11] ed b/lO/‘gb l 55 g 11/ 15;: '3 #3 1.0 l g. erzolc l he. err; i + AGU ‘ .__T _ cf ‘ l ‘ -__ ——— 7 —‘— _ er—Vfi -- WV 7 I HT — 7" ~— A —k -0— '— .__. I 1 MC 1 l i ' l l l i l I I I i ' \I \I I \’ I I I ’ I I i 1 i ; 9cp--.‘ p-.." 7.?7‘"’x.p- I ‘-~- '37p—I-O- pp-—- ‘ *‘a‘f’li'n‘u ’u ‘p. " $1.- 'k'k'k'X'p *‘Lfi-p *7‘:"" ‘- l * } l \ v - 1'1 1 e e/s4/er L 290 'remele 1 Normal 305 “m_____. g 10 e l 1 l i ’ l ‘ I 1 i I; A} J 0‘11 a (1d I ‘ .. 0—.— OII — - an. -¢— -I-c— .- . *‘ec‘p- .- *':€":§.-- *‘33'33‘2‘1- *‘3359'X’; 4352‘?» 5» 5351-536 r I ~ “ r“; t ’1 " i .. v 1 : ‘fl‘é (7/ DC? 2:3 {:1 g :60 t _4_ egrfii 1-1., i emQIC SEC» 1 7. ’ ___ \ “i— _ _._....__,.__. ..- ,_ _____"l:llirr.__etfine1:ationh AG 13‘ 3 .~‘/ ,, 1 2/~~€ee Geo » uale____ a: q 'f k ._Eend_£;fie;, .-.. __._~..___._ ..V .__...___4._.__.__’ . _r. .__-..-/ a4,- ‘. breviation : l 'n" "1”" TH »——-—-r—————-I——’* A Par. means parturition. m [GO—II BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY #IE 1926 FIRST GENERATION 1927 i Date Bi >ru FIRST GENERATION 1%? March April May June July - August September October November December January February i . , U ., , wees->995 was—x— “<- -x-.. .. .. .. meet an: sex-em»: wee-tease: -‘;--:;~::—ee«x- eases-gee aux-aerate m;- %'}1-'§€ 3'»):— "wk-3.- 5/12/20 13 awwwa . , ’ , A SECOND GENERATION 1926 SECOND GENERATION 1927 ‘. (‘\:.)f.\‘fil)nilnfi_8.i V” i Date Born illiys in Iit eroi Sire Sex Remarks Iiegd M areh April May J une July August September October November December January February , " " I i "‘ ‘ — —"—”’—“‘ “ " "“ “ I13 I I 1 t i , e 2 i i . _*__Lle i4/10/22 I 241 I lTwins IUeuu e Gel. l ' l '1 1 I E ; 3 . ‘ i i ' i l ! ”--..” -..--- ~-~CD~ --~t.-~ ----- ..m-. ..---0. ————— -_--- ----- -~-.-— ---~- ,2 ée/ae/ze I 2:5 FemfleT Normal I A AHO _____ ‘ t l I a ‘ ~ I i t , i l I l t I E I 3. ! i l 35 TlO/4/24 291 : , Male thermal '2 IE : . ' I : i i : '. f i. t s l i i 1 y » t i i | ‘ t4 5/17/26 I 1o2 auuw___flrén__a_Deaa,@ delq A, W_Ij,fl_ . gm i a t I i . '. ; l I i l 1 I i l . i a _ , 1 55 ire/1v/aa I 158 L t x Uea e del. ,- IE, I l i l e I ‘ ¥ ‘ 2' i - Third Fienerat on 1 7/14/26 N) {D U) Male Hermal Died 8/19/26 , bbreviation : Par. means parturition. l i t ‘ i l 3 l. i I SUB-GROUP 5 . , OF !_ 5 .r' ;' GROUP VIII (ABSOLUTELY NEGATIVE DHLS) : ‘ l l :5 I I . E i .’ 4 a; 1‘. 3‘ .3 _ i. 3’ -i .11 rtiu,.!ru a 4‘ H H o o o m H m o wH n a He 5 mHHaoa o o o o o umm* o o o o o o o . o o o o o H o o o H H o m mm< o o o o o o o HaHB¢wmzv mack %Dox6 ”CI BREEDING RECORD flé with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1027 :I’-,- . r__ _..._._,’ ‘ } Date Born FIRST GENERATION 11%?)(1 March April May June July August September October November December January February . % ¥ I ' ‘ ‘I I SIX MN!) ('1 iCN ICRNI‘IOf 1926 SECOND GIGNERA'I‘ION 1027 ()I'I'fln'i—ng; M v I D-u'e Born i)‘ w in Uteroi \‘ire l Kiev P(‘111‘11'1'< Herd M'm'h April M‘Iy June Iulv Amulet ‘4<’\)t'(-1'nber October November November I‘mu'rrv Febrmr OW ;\<). l’ur. No. l ‘ Ll ' i ‘ } } A t ‘ M No. ‘ ‘ " ' v n . ' L I - » A , H r v _ - y in §mmufiCmfi;"wmfl'fib_rummwr_n_ _" " _ “‘7””“_ 3“ ‘ l i E I i E g ' ‘ ‘ ‘ ‘ 'r‘ r /( l (W ‘ ‘ 1‘1? 1 a 1 U r ' "7) d "4 .3 - _iwfilrfiL— ; 5% 41; ; i . rim 9 t (-32.11 t: a, .1- ! GI f 3 i i I i r i l l ; ? ‘ m I 1 - ‘ 1 ‘_ _ ‘ ‘ __'_ 2 . 8/ :J, )1 2,91 3 2272 ; Mala ' Dead _LQ, w(1533.. .; ’ a v —-' -—A~~ —— —A» ‘I 5 CI l ' r 1 ‘1/7/24 ' 280 Female ifiormal AHG Tor 3 3 , w ' ----“ -..-..- .-s-—-- 03 ----~ ‘ --—-~ --..-- -"u‘fi- --~-~ --~-- .~ . w --l ----.- l | k 1 r 3 1 ‘ 1 i % 4 -4/1/25 ; 280 j _Ewe Lfigmgle;_£:eeEa/g/25 '.,,_,e-r 2 ,T. '01 ; i i E i . i 1 z . I i ; ‘ l 1 ? I i ' 5 %F/25/26 * 274 * r a ' ‘ m .i _ _ - -__ ‘ 2 1 T l t i .' I —‘d~.h.d- AHG 5...; H \ ‘(O \ K) 0 281 Male Normal (Exg. born)m___ 2' bbreviation : Par. means parturition. [GO—II BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY #DI 1926 FIRST GENERATION 1927 K Date Born FIRST GENER:\’1‘I(’)N 171$? M arch April May June July August September October November December January February r- l- , ‘ I. I I r l l Sl‘l(_‘( )N D (El-1N 1C R.-\Tl( )N 1926 SECOND GENERATION 1927 .. . . if V “ bxfr’)£t\ijly)lllllii.,ii.i \3) l Dill“ BUT” ”£133“ in WT“ fill“ L 90X RPWU'liH £12751 Mareh April May June July August September October November Deeember January February , 1 — ”H ’" “ ““ ”‘ l DI ‘ l l c 2 ’ I . l _ l , . q l 1 :1017/3’ g 275 l 3 Male Neffiai _ T l l l 5 D11 é l l 2 l i i l i i 3 . , = ; ‘ p b 1 2 IZ/BH/du l 1 5 hale Normal __“4 I_.__ l D]: l l a l l g j a | r ‘ i l ' ‘ ‘ ...-....“ -——-- ----- --~'.D- ----- --— --~‘- --—--I -..-...“ T c- ~~in a... l 5 57/4/2é 3 271 l : remaie normal All I ..r ‘ ' ' , : i 91 a l l e i ' ‘ 1 l l I 1‘ ---D-.. ----- -~--- ---------- ----- 1 1 [11/18/25 ; ; s “emare Normal 4 Bee ; l : 1 2 — *‘ *‘“* ‘“*"‘* ' . ‘ ' ‘“ i 1 z 1 l t g i . l _, N , ; Tnlr‘ uenerat10nflfl_fl 1 fl 77777 All Cow threw herself and l I, / ' V 2‘: R Fem-C; 1 3 913911th I ma _ {I \. Iv . l i l l ,i__.i-i__-_ - A _ l 1 l l I i M j breviation: Par. means parturition. 1 160——II BREEDING RECORD #HC with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1927 A‘ Date Born ‘ FIRST GENERATION \ liege March I April May J une July August September October November December January February z 1 l I, l l l i 1 l ; , . ' 12/26/16‘ ‘HC ¢¢wua l -~—-- --—-~ *---- ----- *--~~ pp--- --~-- --------------- ----- SECOND (llCNlillA'l‘lON 1926 SECOND GENERATION 1927 ' .. . , “ “"'“ i ‘$)l*\l:1)).1'1111<§”ol No. \ Date Born ‘lmys in Uteroi Sire L Sex Remarks liegel Mart-l1 l April May J une July August September October NOVOIlllLX‘I‘ Deeember January February 7—‘ “_ I ‘ i AAAA ”I "’ _________ _“fi‘m ”‘ 1 HQ * l i i 1 ‘: 2/2/3.9 , 287 a Femel e Normal HH ; l | l HG '; l l l 5 1 ‘ g ‘. ! 5 l l l l y ‘ ’ 2 l 1/21/29 l l lFemale , Normal __l IB __ l l I l HO l l 3 l l i , l l a 2/10/21 1 285 l 3 Male Normal ‘ 5 l HO 1 l l l l g l l l ‘ i i 4 1 4/7/22 1 280 ,__ ____,_Nomal___ _o r_ t; l HG l ‘ ' ' l . t r _ l l l 5 l. 5/11/25 285 T Mal 6 Normal J _w _,_ , __zffl _ \ HC 1 l \ HG ‘l 5/16/24. 28'? l T Female U1 ed6/29/%4 'f 7 1 6/1/25 2923 l Mel 6 Normal A HO 8 8/15/26 297 Male Normal J _P_I Third generation l HH l 1 5/21/21 282 1 Male Dead €23 del. i \ HE ‘ ‘ l l. l l .I a i g l 6/7/22. 28; l lMQle Dead @ del ; l HE E [Male 1! l 5 l 4/24/93 275 l Female Normal 1 l. HH l ’ ’ " "V i l 4_ l 8/2/24 277 Female Normal BOG l l EH l f ‘ Died 2 mo . g 5 ‘ 9/18/25 289 IF after hirin. 4 l l l Fourt Gerrarwa’r i on . BOC l o k I 1 1/50/27 254 Fem-1L Dead @ del I to breviation : Par. means parturition. ___. _Mrw—qu.d .. ....C::V——_i:1.‘~i A - ..-, ii 160—11 BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 5H1 1926 FIRST GENERATION 1927 l! Date Born FIRST GENERATION I119? M areh April May June July August September October November December January February I S'ICCON l) GIGNICI—L\'l‘ [ON 1926 SECOND GENERATION 1927 011* —' - r T H '1 aw {(131 “W; No R Date Born Days in lit ere \9: March April May J unc July August September October November December January February ' l HI l l 1 1 58/21/21 3; g -3‘-%1~p_- *---- *---- *---- *---- *p-nu- p-- ~- Mp-.. téié-té'k‘T *‘X-pp— flux-7‘64 d fink-kp- I, - .. _ , . 2 le/ra/es 2% age III F l l i 5 iS/V/Qa XIA a%%*- a%%p_ *p--- *N -_ *%*-— %%%p- %+%e% *%%%* %a%*% %%m%, *%*%% mnw%% III E l p / /(\r Killefio l 4 i 5,14,60 Deformed % i . l l l r t i ran Generati AUD 1 8/17/25 ' AIA 1 8/7/26 3 breviation : BBH p--~~ p---- Par. means parturition. 1 3‘2: . r n 160—“ BREEDING RECORD #AEE with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1927 ‘1 Date Born \ FIRST GENERATION 1:931 March April May June July August September October November December January February i l l l f A l i b/lO//82 ! IXEE -nr¢:~ --.-..— ---—- ----- -—--- p--—-~ —--~- -"--- -"--- ---'-- ----- ----- i l SECOND GlCNlCRA'l‘lON 1926 SECOND GENERATION 1927 mimyilnimflfii Yo \ Date Born [Days in Utero Sire Sex Remarks liegd March April May June July August September October November December January February A . L . A . ‘ l V._‘.- i r 7" A o — . 1. l l i AEE ‘ ‘ i 1 ‘ - v . --c ~--- -..-...- p—-——— pus—cu ph- - - -...- -o-v--e pp-.- :- p--- l '_____iLer:1crlL‘ ‘ " “ - Norma l AIIJ p p l 1 l l ‘g l r ! / r . l p I p---- pm- ----- .23 l 4/20, 25 276) E gr ema 16 l‘lom‘lal c In 131m _, u l AER l ‘ l g i a 3 1 4/6/26 276 lFemale Normal 4 ; s I 2 e t l ‘ l l i i __,_____v,_*‘- , _ _ - i i Third Generat Qn AID l 1 9/8/26 281 Male Normrl.“yealed ~.' . breviation : Par. means parturition. SUB-GROUP 3 OF eHOUP'VTTI (Dans THAT BECAEE anaeroas) ND l'p’ '7i3 o o o o o o o m o o o H ¢ m mqraoa o o o o o o o mmma o o o o o o o o o o o o a o o o o m mma o o o o o o o mama o o o o o o o o o o 1 o o H o o 0 4H m H3 H5” o «5% .mo& twoz .mmzm .Qmsn .mom owez unoapnon< modes moawsom nonEom .adm Elm Op 0» Hweaoz commooom oopmop Ho Ho . m oh . mlom dunno? IGDI . o? . oz uOHdEom .GOD GAHSB meamfiom .cwc wcoocm mwaouwm mzwnmuhm.kwwfigm Hausa O .hh dam. 5 mowogm gem ccw Ema 0gp Mo ”woe :OHpmsHpsHmm¢ on 66:6a6e6m an“; meaootz.mnaemoam m Tax/H838 margin 84:3 mafia MSHarmmzv NHw m 30:3 ISO—II BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY #A El 1926 FIRST GENERATION 1927 \ Dut e. Born ‘ FIRST GENERATION 1:9? March April May June July August September October November December J anunry February [ I i ‘l ‘ 9/21/33 ‘ AEI ----- ______ .__--- ___-.. ..... -__..- ..---- ----.. er'wx- sex-7.1», #30 ~ am e:--;:-,;--;:--,: 2 l SECON D GENERATION 192G SECOND GENERATION 192; OfTspriue; of A I . T .1 Herd , . v ‘ No. I’nr. N0. Date Born ‘iDuys m I; tero bu‘e hex Remarks I To. March April May J une July August September October A ovember Deeember J :muary I' ebruary L 1 I £ T! AEI 1! ‘ l l 1 ‘ ‘ i ‘ | —-~m- ...-.....- ————— -ao-~- —-—-- ----~ ---------- —-‘-- . 1+3; 7/35 I, 277 % LFF-zmale I‘nrmal 3 BBF ‘ AEI 1 i E I \ l i I } a ] ; _L2_-L e : r“ m 5 . r ‘ l i ; I I t j I y I ' | 1 i ‘2 i ; ‘ 1 g I 4 r % ‘L ‘ \ I i I i ‘ t . ‘ ‘2 ; i i ‘5 i 5 1 ‘e ‘1 I i i k ‘ 4% I I {L : _,__- _ ' l 1 - . I i ‘ L: I. 1 a = l I i i 1 i I * I I i i ‘ breviation: Par. means parturition. ‘.Itl . ' #AFH BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1927 l Date Born \ FIRST GENERATION 171$? March April May June July August September October November December January February : l l l 1 l 1/9’1’253 AFH “an,” ...“.-- -.. ...... .... ..-- ..... .. -... .. amatee-x- annex-e: -;:~:;-~;:~-;:-v:;— ec-ee'xwzses- n ’37 an. '2 me I l l i J SECOND (’ll‘lNlCI-{i-VJ‘ION 1926 SECOND GENERATION 1927 Offspring of W \, . \ . T, w , _ _ I'Ierd F T ‘7, 1 A ,-1 M , v _ w No. Par. No. Lat e Lei u Lays m I; tero b; re bex Remarks No. Mal (- 1 p11 , ay June. July August _ September October I\ evember December January I' ebruary l l _. ——< _._ . ___V ,_ AFB l l a I ‘ l l '. I _Mo-gn— ___-..- ---—-_ -o—o—o-e— ----- anon—Inn- 1 L 8/ 4X 2 5 l l P ema l a Ii 0 “mi a l BEE l ‘ AFB l} l l l ' if?" A | 1 1 ~ 3.: _‘ l ‘ 2 l 8/161” :16 , M23413 11 O rma l j y f I l. : I T "— l ! ~ i : l l. l -. "Wm—am”--- i l I l 'a breviation : Par. means parturition. GROUP I" A. (IMMUNE FALILIES) POSITIVE 1111:1513 E‘rirIIIl..Y I; It USO—II BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1927 fFG Date Born pf FIRST GENERATION H?“ M arch April M ay J unc P 1313‘! l E: I: n rev-1 {fl .. ___—«M-.- . -—..—,—-r~7 bbreviation : I\ 0 July August September October November December January February Tr ~ /r* « t " ’3 fl!“- ("\ t1"! \ ‘ \r 5' I' 15.1 11 ed AE/b/ d b __ y - - J. if 'n‘ 'If'tv'n 'n‘ SEC(‘)ND GENERA'I‘ION 1926 SECOND GENERATION 1927 Offs arin:—-;; -)T -, -);--21--)i--21-~) ~ -3(--)& 99:2"): 61-0. 7 6‘ - -3 ~-;; 46'7“} ~\ (- ,‘ A -.‘ ‘ E \ \ ' t . _ V /' T ‘ --. I 1 b ‘4 . 3412: 4/2: /‘19 L :3 I] :1- nl ’ 'H' Milt-7‘," 15" _i' “xwwvri‘éil __| ;;:IL‘[ _"_____. 1 ‘ JV :7 l "T 1 I ' -- --- '- T' L515”?- | ' l t t i ll 0: \-" ‘ ‘1 ‘ I 1 t i l l I \’ ’ A I ’ ’ I r 1 t 4? e? [#017 u ,e l or”? \ I t I ‘1 ’r‘ j , " p i 5.! t 2); I; :~' 1" L. - 1‘ r a T * «'- L u ,L L» l * ~4- ~ _ t l Ll‘vr‘ l I t i J» J t t t t l :- = ‘ i ' i t ‘ I I-‘L;‘.\L ;',.\-"r_.‘ -|-'-. - I. .U_ “>.r.\l_.li‘ \_\ w \- \' s/ K . \ 1r ... .. \r .v r . t ’ I I". ‘, ‘ 1 ’— ‘ l‘ l p t a p. l‘ «- an... a. :- an‘pc-n .- 0. '3? n n I\ - if ;\ u: u n n X *‘z: )1» n 'u u n n '4. 'r- ’n’lf'n‘ fly'fl ',.“,‘ 'n- 7;.“ 7:7;- -;‘ .t "n -' oh.“ .;;..;\. .3839? 1 {tr t by ..'-i V." 43:" t ’ if,“ i l J" L L116 . Fifi? t 1 t 1 i “ ' " l t i t t t l i : t ' t t t l l l l l a t l t J, l _ -_ , ___ _ - l» t t I l I L T t t ‘ l t 1 my. _ ~ 1 ‘ y . t IliPC Uenena ~_Lon _ H ,_ _________ M -or ' 41 a 3 / , I ~ i 7. -1“ ‘54! 1 ;1'. i/ g; 1" 2:: 5 1Q a {a I 1 x: 1‘: n raw 3 .1 ' I J“ L, ; a“ w .. , . , ..1— AI}: n. . .. w B/L/Z? Par. means parturition. racer _I‘: T'pw FW'V'Y'T“ V1“ 1. J’Lré‘a‘lVJL 1.1..IJ-AI t4 FAITIILY ‘il [GO—~II BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1927 Date Born FIRST GENERA'I‘ION 229:)“ M areh April May J une July August September October November December January February I i SEC(’)ND GENERA'I‘[(')N 1926 SECOND GENERATION 1927 , Oil'spriua' of t D‘ ._ l‘ . I . . T ‘ ‘ q ‘ ‘1“ Herd W M" "l A ..1 i M J J 1 A ‘t q x _} ‘ O 't } ' N Y \ l H D ‘ }> ‘ J‘ ‘ .7 F x] ‘ ‘ 0“, No. I’ar. No. i Me .50111 [Day s m IIIOIO hue t ex Remai \s No. air 1 p11 j ay une u y ugus .. ept< m Ma Q 0 )Cl i ow m )(1 (0011] )(‘I anuaiy ( )iuaiy L »—‘ ~ . 5.31 l t I l l ‘ a 6|- :3 m a: - a. “u :3- i ------------------------- L t r" /r- In. t r‘ 1'} 1 ~ 1* 1 'h\ l l -------------------- .__-..- _i,a+ t 3/3/49 1 57A; irwnfiaz 1 Marina AEC t #1341». l} t. 1 F i i r t ,H ,e” g a i t” s , ,w : -~-~- ------------------------- -~--~ ----- l a t EyES/EQ 4L 87b l ire, 1e 1 Berna] AGD ! W137 t i = ' — r“ ‘i ' “”* l 1 t t l , i t . I i l y I I CBC-cos.” -..—.... 5 --------------- -"-- ---‘-— --v--.. ...-”.... ----- -_-_- -__-... I '2 i 42 /'! '3 x/n/‘s 3H? ‘ "Ia- (. A I , . .1 l «I i , p l U E u; may (It-.- . a («‘e.) 1 l:- ~*l;.ln.- t In? ‘ .3.“ ‘AIl’b ' “rt-EFT ‘ t . t t —- f. 1),,1- 4.. L t t i l , l' t i i t F / l t ' 1 ~ i 4 5’ lif/Q'S 'l 2&74 t; 'yfif‘zqfll'n ‘ 1\O_’r1$1‘, 1L. t f : ----- -----. a—uuno-I-cu ----- ...-I.- *---~ %.*PT- *ée*-- i;'*—-- *p--- 55]: t l “I i — _ I“ FF I 3‘ - a I g i g a _ i 5 0/4/25 s} 279 t Perez 15‘ Normal t t 'rd Gene: ‘ati Q'n 6/15/24 M21 8 paw 5/18/25 [0 Q y. p. NQ‘Pm g] i“ 52:] mm 0 A ”___—A [A 5/21/25 1 AGD 12/8/25 275 Win-IA vIerMQ'I U ‘l .... r-\'\*Kj hYUJ..'FLu.L ' 274 l“ email 6 N arm a]. 2/21/27 279 . F remake; Afiormal baled BCD Normal U! 0 H I - h a.» .__,I—._.—__ _,_ .__-M_T_._._. _.__-4_i_._,ii . - ---_u .fi'ifié..- **--- pT-~- pT--- DD"""" 4%---.- pT- una- ?. bbreviation: Par. means parturition. 1. 2. 3. 4. 5. 6. 81 SUMMARY OF SEROLOGICAL STUDIES. The animals that had a Bang titre at the start g£1the test strong enough to class them as positive (Group._l) were positive at the end of the test. The animals that were absolutely negative at the SEQEt of the test and remained negative during the test (Group ill) had the best histories before and during the test inspite of the fact that severe non-specific infections existed in the herd. All animals that were negative at the start of the test and then aborted due to Bacterium abortus (Group 2:) were spotted by the agglutination test before the abortion occurred. Post-Parturient Reactors. Six animals in group 2::became post-parturient reactors. Three animals in group 911 have already demonstrated that this group is evidently immune. The significance of this data is that we find a possible explanation for the five plus reactor that has never abort- ed. Evidently organ immunity exists in the uterus. The organism has been isolated from the milk of the three animals in group:EI:. (Milk data obtained from Mr. Torrey.) Any sign of Bang titre in a previously negative animal should be regarded with suspicion. The fore-running Bang titre of several aborting animals in group 2: was low. Imune Families. (Group E) 1. Positive family--no abortions. 2. Negative family-~no abortions. The negative family is probably immune to the particular abortus strain found in this herd. There is probably organ immunity of the uterus in the positive family. Bacterium abortus has been isolated from the milk of all lactating animals of this positive family. 82 STUDY OF FDTAL hfihbhnLEs. Plan of Procedure. Group l Normal fetal membranes and their anatomical variations. \ Parallel arrangement of cotyledons, A. Semi-,arallel arran'ement of COleedons. i.) Irregular arrangeWHnt of cotyledons. B. Presence of accessory cotyledons. C. fresence of naked areas upon crorion. Group 1 A. Fetal membranes from cows with history of disease at prior parturitions. B. Lesions in normal fetal membranes. 0. Diseased fetal membranes. D. Consecutive membranes. Summary of fetal-membrane study. 0.. r _ .H..Nd..,§.§.fl§-.;§E~ .‘iu .vnb . 11", . 83 ‘ i m * r ,‘ Paul. 1 _ r'1fl1Tf1 71 11 root") 3“TT‘|(\ Q-LU] (51‘ 4;.2‘IJ iA-Lih ‘ l‘mx¢o 3J|J. IIWTii.J7KY‘IC‘ By fetal membranes or afterbirth is understood the allantois- chorion tith the amnion. The fetal membranes Were r :Cord d to obtain charts of consecu~ tive parturitions. This we 3 done in 0rd r to observe possible differences in normal prrturitio s and to obs rve t”e results of lesions soon in diseased Hombr:1:s uron the subse Qilcnt membr nos. To accure.tely record these nembrrnos was a difficult problem be- cause of the lack of facilities to do the work. Photography ex- cert in special cases was out of the question. The charts herein recorded were made by directly tracing the fetal membrznes upon trecing paper. The membr: nos VJHI'C out along the umbilical veins and spread uron the floor which hLd been previously cl<:tncd and disinfected. The memban“-s were then covcrrd by a l rge f emcd glas which would take care of any width, but had to be shifted for the length of the outs stre tched membrixnos. P151011 IllEiWCJHtB l E.I"Cc. Wits (_iil‘i ECtly tr; 0:311 urjnn tie :35??? through the glass. This gives a fairly accurate roco ord of the exact size and location of anr n a ontal arcs, internlrcontal 6nd rlaCental lesions otc. ‘he umoilic a1 veins Ind arteries Uwro recorded to th e extent which rould indicate :ir location. When the outline of are 3 to be recorded had been H( the 5M ss was removed and each placental area individually €Lt11“vfl und :r a flood of Wat: to determine the presvrce of disease. The i torrl Conto 1 grm 0 Were likewise examined fcr extent of l~oinns, (f The necrotic glrccntal are s More jwdicgt’a in yellow uron the ‘ r ' 0 w a 0 Chart . The int ( rplac onto 1 lorricrs \IJ: re 1 id: c: t 1":(1 in White . Guinea rig inoculationsC more made if necrotic rlecrntal areas 0 o tx>GENERATHX< Mn? Ofl'spring of l , . . ‘. Q 110111 . ..1 A '1 M J l X S l ' O- l ' \" ' l ' D ‘ '1 ‘ " I‘d ‘ ‘ 0w No. Par. No. 9 Date Born Days :11 IliOl‘O Sire ..e.\ Remarks N0. Mare 1 pm ay une. Ju y 1 ugust ._eptem in (to )01 l m em in emu: )er January w. )Iuaiy lei { l l l i , , i I .' , , l n . ~‘ . .. l a li/eO/l i 278 1 i Male: .Ulfiv 1 _ AI ‘ i l l l o . ,, » . r g » l- x a 1 e 370 ‘:/l@ . all g ; FemalelDead L oe“.; ; , I . A: _ _~ A1 i l i 1 \ 1 ‘ E l l, l t l H i l f“ A" i n i ‘ ..' , I »- ‘ ‘ . 0 /<;41 ‘ ~77 ; hale :hnrmal . 1 1 l ‘T i I I . AI 1 1 i a . l i : l ‘ i e:eee.a;ee eeeoe;e:¢e i eeeeeseeee e| t . i 1.. .I: 1 . it]! . \ f i . r . 7% .II. BREEDING RECORD Cow No...M..BEI ...... Breed. Owner. Name. Date. Reg. No. Has animal been tested for infectious abortion? IhmBmm. 12/18/23 Age to Date. Date -_ Reaction.-..Nega.time...-.. _ PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Heat Dates. Dates Bred. 4 / 4 /26 Parturition No. 2 . Date. 1/10/27 Gestation in days. 281 Hours before passing?.--.-..---- Lormal ............................... Aid? Fetal Membranes. Treatment? the r t mad 0 Condition? Iiemangioma of f e ta 1 membran e s Disposal? Normal? N 0 rm 8 1 Vaginal discharge? Diarrhea? Sequel to Parturition. Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf N ormal? N 0 mm 1 Sequel to delivery. Dead? Die? Date and cause of death. Sex Of calf .......... Male. .................. Post-partm'ient History of the Cow Parturition Date. Post—parturient Heat Dates Post-parturient Service Dates g 1/10/27 9 0 I. ..i. 'l viii". 11‘ [I . N y I? . . I r . . .. . .. . 5 | . . git ...”... lnrz-.mf...... .- ,. -. - .- . -. . . . u. I! . . i - _ .. .i s _ llal . . . D. . I .O 1' 1.. HI ,5... i .. I ‘Il‘ll . 4' . a. . cl lien... i.’..br.--tuftlwul.flp E. u l A . I‘ “§ I i v 1“ . 1 i .1; ”H3 1 I 'btnsfi‘j I .305 I... ’DW’IE, IR .‘lhlr ...“! {BS-u.” T E ...!t \r’ 51", .. t..- w . . . _ .. ..I I. , . I; ..LanI. -..-.0 ’F10‘ 1 .1 ... a1. . . .. . I. . f . . IA $s . ... .. . .k/ 0. . 11‘ x. . v . II | D i ‘n . ’ .a.I‘ ._ '1 5"! v :4 \l . ~rl axf‘h.ll ..‘nnd. l ) CHART #M BCF OF GROUP _1; A Study of Placental Arrangment. 1. Apex of gravid horn. 2. Umbilical veins and arteries. 3. Cervical end of gravid horn. Classification. Five semi-parallel row arrangement of placental areas. :2 Form 162—41 Breed N am Reg. No. Date Born 4/]. 4/25 BREEDING RECORD Cow No..-M--.B.CE ........ Owner Date Has animal been tested for infectious abortion? Date Reaction . po 31 t 1 V6 BREEDING HISTORY Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 2/29/25 264 Normal 4/3/26 280 Normal Due May 1927 Par.No. Heat Dates Dates Bred 1 7/8/24 2 6/27/25 3 8/13/26 I l_____\\\\~_‘ .MMmfiukmm. Par 1:: Ga, "1% parturition or calving. gestation or number of days carrying calf. Form 161—11 BREEDING RECORD wrrn REFERENCE TO INFECTIOUS ABORTION Cow NO..-M-.-B.QE._--.-. AGGLUTINATION TEST . DATE . Blatiatlb): 3f Heat Dates Dates Bred Par. Date REMARKS 1925 Blood Milk Colostrum -3 r “3 hr. ----- 3/19/25 Normal lily ----- 6/27/25 July “D.- Aug. «us-m Sept . W 00130 *“p- Nov. 9%“. Dec . 49% 1926 Jan. 49% Feb. “pun “‘1‘. '~ **--. April pp--- Pos . in Colo trum 4/5/26 Normal Straps also n 601- ‘ ostrum. Neg. to B. abortus in m mbrane . lly *MP July Hat-mt» A113- W 8/13/2qi Due May 1927 Sept. «was» Oct. MHz-4H- II..T---- Not attempted. 1 .T'I‘--- Nov. “H- .T---- Not attempted , .TT--- .T---- .me Dec . mews-p .g.--- Not attempt: .T---- CDT--- 1927 J an. *‘IHI-pp Feb. Han-p 1 Abbreviations: Par. means parturition or calving. -- o‘.~.- '_--'_<‘ - l'. ...J -- _ ' r - _ by W--.» — — . ‘ ~ 4—4-1... .-O.. . - l . --.. . 1. . u V1.4 74,," i\ .i. NdLV...Anlu.\ .1 ‘ ...“. .Gumt‘, WI. . CHAMT -/yf BEB OF__ GROUP A A Study of Placental hrrangement. l. Apex of gravid horn. 2. Umbilical veins and arteries. 3. Apex Of non-gravid horn. Classification. Five semi-parallel row arrangement of placental areas. 1 1‘! “J Form 162-11 Breed Name Reg. N 0. Date Born 10/15/23 BREEDING RECORD Owner Date 1 Cow No ..... M.-BEB----- Has animal been tested for infectious abortion? Date BREEDING HISTORY Reaction N ega t 1 V6 d Par. No. Heat Dates Dates Bred Sire ‘Par. Date Ges.in Days Sex of Calf Sequel Of Parturition 1 2/6/25 11/1/25 268 week calf. Died in six itrs.’ Cleaned in 5 hrs. 2 4/14/26 1/14/27 275 Female Dystocia. Abbreviations: Par. means parturition or calving. Gas. means gestation or number of days carrying calf. 1:) .l m o ., . b, .| . o v ., tlllltllnilyl ‘ ill-III. £9.11: 1| .v l"! a? . .1 o r Iv'vtcil. I I! I i BREEDING RECORD Cow NoMBEB Breed. Owner. Name: Date. REE- N 0- Has animal been tested for infectious abortion? Date Born. 10/ 1 5/ 2:5 Age to Date. Date Reaction"?!Q55331M9. ......... PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Heat Dates. Dates Bred. 4/14/26 Parturition N0 ..... 2 . Date. 1/14/2'7 Gestation in days. 275 Hours before Pas-Sing?"NoKaela-passage-------~---------- Aid? Dystocia Posterior P. embm'neS. Treatment? Ch . , art mad 3 Condition? 0 .11 . Disposal? Normal? Vaginal discharge? Th? 6 81 t on Cd Segue] t Diarrhea? ever Si on O f u t eru S 0 Parturition. Mastitis? Milk-fever? (given Chloral ) Pneumonia? Septicemia? History of the Calf Sequel to Normal? N o rma 1 d . . ehve Dead? Die? Sex of calf Date and cause of death. "““‘~-~F.‘-emale ............. \ Post-partwient History of the. Cow P .\ -... won Date . Post-parturient Heat Dates Post-parturient Service Dates 1/14 /2 ,7 \ ___—___...— VJ VJ CHANT ,9? ADC OF_ amour }_ A Study of Placental arranéement l. Apex of non-gravid horn. 2. Umbilical veins and arteries. 5. Apex of gravid horn. Classification. Irregular arranbement of placental areas 0 1.10 l‘ “J Form 162—11 Breed Name Reg. N 0. Date Born 8/2/21 Owner Date BREEDING RECORD Cow No...AD..C. .............. Has animal been tested for infectious abortion? Date BREEDING HISTORY Reaction Nega ti ve Par. No. Heat Dates \— 4 10/2 5/26 Par. :11 ‘ Gang parturition or calving. gestation or number of days carrying calf. Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 6/21/25 12/2 7/23 282 Fema 1 e No ma 1 #AHE 7/4/24 8/29/24 6/8/25 282 Male Normal Sold 9/8 25 10 7/25 7/11/26 277 Female Normal 9/50/26 * lllr -ll’l In. ..yli ..ltl‘ll‘r- t‘l‘iutbylbh. 1:. In v: BREEDING RECORD Cow NoADC -- _4 a...__.__.ui__——L ‘—— ' Breed. Owner. Name. Date. Reg. No. Has animal been tested for infectious abortion? Date Born. 8/ 2 / 21 Age to Date. Date _____ Reaction---ll.eg;a.t_i_1r.e.-..--.. PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Heat Dates. Dates Bred. 9/8/25 10/7/25 Parturition No- 5 . Date. 7/11/26 Gestation in days. 2'77 ' T 17‘, r Hours before passmg? O I ..lal ........................................................... Aid? Fetal Membranes. Treatment? Condition? Disposal? Normal? N o r tn 2. l Vaginal discharge? Diarrhea? Sequel to Parturition. Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Normal? N orma l Sequel to delivery. Dead? Die? Date and cause of death. Di ed 10/9/26 Sex of calf ........ Female .............. Mechanic a l Pneumonia Post-parturient History of the Cow x K Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates 7/11/26 [Z]. CHA H'T [)5 AFH OR_ GROUP l A Study of Placental Arrangement. 1. Apex of'non-gravid horn. 2. Umbilical veins and arteries. 3. Apex of gravid horn. Classification. Irregular arrangement of placental areas. 122" Form 162—H Breed Name Reg. No. Date Born 1/9/25 BREEDING RECORD Cow N0 ....... A EH. ......... Owner Date Has animal been tested for infectious abortion? Date Reaction Neg. until Sept. P080 in Sept. BREEDING HISTORY Par.No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 23327:: 2 10/17/25 11/19/25 v s 10/11/2 11/20/2 12 15/26 1 6/27 1/28 27 2/5 27 2/17/27 8/4/25 505 1 Female Normal #BBE 8/16/26 271 Male Normal Abbreviations: Par. means parturition or calving. Ges. means gestation or number of days carrying calf. BREEDING RECORD Breed. Name. Reg. No. Date Born. 1/9/25 Age to Date. Cow NoAPH Owner. Date. Has animal been tested for inf ectious abortion? Date ........................................ Reaction. -.Neg ..-..an 1111.. Se pt . P08. in Sept. PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Parturition No. 2 Fetal Membranes. Chart made . Recorded as a non-lesion neg. Sequel to Parturition. Heat Dates. Dates Bred, 10/17/25 11/9/25 Date. 8/16/2 6 Gestation in days. . 2'71 Hours before passing?-... "Normal .................................... An? Treatment? Condition? Disposal? Normal? Norma l Vaginal discharge? Diarrhea? Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Sequel to delivery. Sex of calf Male. Normal? N orma 1 Dead? Die? Date and cause of death. Post-parturient History of the Cow Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates g 8/16/26 10/11/26 11/50/26 12 15/26 1 6/27 1/28 27 2/5 2 ”Iran ——-“‘ ”I I! ill“ lill‘;.l‘l l a. ... 1 .Jr’..; - VII. IV] oli'vf .dez'--.."!o.l’, . tail... . . . Ar. title'ta .u Jr ' 0"..‘1‘ -‘A‘. ‘1 «.7: Form 161—11 BREEDING RECORD WITH REFERENCE TO INFECTIOUS ABORTION Cow N0 ....... AEH ......... AGGLUTINATION TEST - — DATE Blsoiatlgn ff Heat Dates Dates Bred Par. Date REMARKS Blood Milk Colostrum ac ‘ a or us .4. :1 4 fi fi- Mflr . ----- Apr . -..--— M‘y Qua-un- 1 .5 i s 4 ‘1 t 3 Sept . *4é'x—6Hé l 0013. ***** ---~- 1001;. fast test pp--- j wa s it"s-)3: *fi'-.. .t ‘Iov . taxes-2:4:- 1 Dec . **+(-** A 2927 ‘ u an . 43°23'31"; '9" i t F eb . mete-H- Abbreviations: Par. means parturition or calving. ‘l lu 160—H AGGLUTINATION TEST OF THE DAM AND HER PROGENY BREEDING RECORD with reference to the 1926 FIRST GENERATION 1927 Date Born ‘ FIRST GENERATION l 119:;(1 M arch April May June July August September October November December January February l l l ‘1]? l3”: i in}; I, t 1,]! ,‘3/ ‘1‘) ‘ 'n-H anew-acou- _muuu- “-amo. --~-~ -O-¢--- —~—~- **e{';b~yf ‘i'c’3\"3."}$"}i‘ ';;";\ ‘2.”2323 '}(-'..'3C’)1'X‘ '}("3r‘i 'Ih'n’ eeey 'Iéfi‘f’" i SECOND (‘thN’lC'l’tAil‘ltlx i l l l t l l Ollspring of I . . 0W N0. l’ur. No. Date PM“ \Dill's‘ in View Sire ‘1 Sex Remarks {Read l l AFB 1 1 l ! i N I / . I! i ‘r 1 I .V ‘1 K 13/ 4-; 2'): l 10 in '3 i l .L‘ renal E: ; 401771211 BBL AFH 3 l l 1‘ l ‘ g /1 A ”z" ll ‘ ! l l 3 8; 1e, 2t 1 2'71 Lit-:1e 1; mal | i ii i t I i l l i l l l l i l l I l l < ‘ we ”.__-._pqm i 1 l l ~t ‘ bbreviation: 1926 SECOND GENERATION M ureli April M ay June August September October November Deeember January February _ --.»...— — —-. ”“393” ~_’-.- -_‘u’. ““‘““l‘ ,1 -..—__t- _ ......_D.M~.-A L--. .---.-_..- _~_-_, __m.r.._ ——_. - . Par. means parturition. 127 . 00' \o. ... I: ¢.i’- II 'v .g't: JJJ. . . o . , 1. I \ 9. u .. .a’. . all t . I - '0r .\ O . 1| '1‘ ’1' )2‘....‘. o .73 07'! 11‘"- to}... 1 .'i atlas ill" . JV: 1’» .. . O ‘ .i". It... 'li‘Illl‘l I 0 It i 7 Q ..I I. D ’I.‘ it . l . t . t I a. e i r i . .ii ii .v rl i. b Ii . n‘i. 404101,: ‘4. it . ftbii.£‘£: .‘ . ii 1 . ..l t 910‘..‘ Uiii it.llla:l’ 5! I. . [it El. . . ,:I ll». . u I. . v 0' t -34).-.. 1.. .il. iUnHwidi - t. ....o I l i .10 r I..' s... . AI lull-‘7’ llltll 0' I CHanT f AFG OF amour 1‘ A Study of Placental Arrangement. 1. Apex of the gravid born. 2. Umbilical veins and arteries. 5. Apex of the non-Crevid horn. laccnta from a Jersey heifer following a normal parturition. First calf. Note the; few piglet-311,221 arm 8 in the bravid horn. I, 1’? ... 1 ii 1 iii .11. t til. t ., if x . .f .....Irvl 1. ti. ...-II t .I. lit . . vi. . ... u]? ,. ,t’. I ’1 - O. .. I. t‘ eel-O'va Y ’ 3 .’ .. 1 p. . 1" ..7..J (r.." l I! .XlIHviflIu.\ h ‘ t l u r Flu; . r. 10'. .4 a i lit :91: B n. 3?? {I c 3 I .aa‘l 47 |...Dt!l‘ 0.1 c e. 4’. v .I It ll 0. ' DP . ..a PART B OF GROUP 1 PAMT B Study of Accessory Cotyledons. CE 1.30 «...... - ( my‘fi ' ‘1‘t7V o‘.’ ' _ 5 i v a " ), ..K’ I l L ‘J t J t O i i .-l - ...-...- o .... w ’ 1' 1'!" ‘- -“1—4...“ ”.1- romI-‘i m-- w “t-m" 3-“W‘Wuvfi A‘mm‘. "l :4. J I.“ .‘i. 3 ft ‘3 I st ‘t Ii .- m . ..‘1'1‘ ‘34... V’IVW r .4: ii i .--- - -| t -u a. t'fi‘ ”‘W‘ H .... “~w- Y "Q .. 9‘ a 7" * 1%.--- . v‘ ‘W'm “figfi 4*..- *i‘iVW“ . .... ¥- 0 .M' *~ .. A ...c. .... . -———-—. CHART # AFA (1) CHART # 5E3 (2) Photoaraphs used to interpret accessory and diffuse placental areas. AFA (1) AFA (2 l l. Cervical end of non-gravid horn. 2. Umbilical veins and arteries. 5. Apex of non-gravid horn. 4. Several accessory cotyledons. 5. Dense group of accessory cotyledons. 6. Diffuse placental area. Gravid horn of same case showing, (4) Cluster of accessories. (6) Diffuse placental area. Form 162—11 Breed Name Reg. N 0. Date Born 39/13/20 1 BREEDING RECORD Cow No ...... AFA..--...-.. Owner Date Has animal been tested for infectious abortion? Date Reaction Po 8 1 t 1 v c BREEDING HISTORY Par. No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 11/19/22 Male Normal Sodl 2 5/22/23 6/8 25 12/14/25 x need at delivery 5 4/11/24 5/22/24 2/24/25 279 Feme1e Normal (BAD) 4 6/1/25 2/10/25 265 Eema1e Deed at delivery Retained 39150 cnta . 5 5/15/26 2/18/27 Female Retained piecente Abbreviations: Par. means parturition or calving. Ges. means gestation or number of days carrying calf. 134 J' ' 1“, “‘al.d‘tt . ‘..‘i. ‘5' . in". CHART # ACG OF__ GROUP .1. B Presence of Accessory Cotyledons. 1. Apex of gravid horn. 2. Umbilical veins and arteries. 3. Apex of non-gravid horn. 4. Accessories throughout non-gravid horn and apex of gravid horn. No history of abortion or disease. Form 162—11 Breed Name Reg. N 0. Date Born 2/12/21 BREEDING RECORD Owner Cow No. 16v AQG Date Has animal been tested for infectious abortion? Date BREEDING HISTORY Reaction Po 3 i t 1 V6 Par. No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 11/18/25 See 7/14/22 8/23/22 9/11/22 9/30/22 1/11/2e 2/2/24 3/18/24 5/22 24 9/4 24 11 12/24 11/18/24 10/15/25 notes '7/51/26 7/5/25 5/5/26 277 63/10/58? 280 Male Female Female Normal Sold Normal (Died 1/8/26) Normal AManMmm: Par. means parturition or calving. Gas. means gestation or number of days carrying calf. 1.37 BREEDING RECORD Breed. Name. Reg. No. Date Born. 2/ 15/ 21 Age to Date. Cow No..A.Q.G......- Owner. Date. Has animal been tested for infectious abortion? Date ........................................ Reaction-.--PQ 8.1 Live. ........ PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Parturition No. 5 Fetal Membranes. Chart made of olacenta. necorded as a non-lesion reactor. Sequel to Parturition. Heat Dates. Dates Bred. Date. 5/5/25 Gestation in days. Hours before passing?---....Normal ............................... Aid? Treatment? Condition? Disposal? Normal? N 0 I’m a l Vaginal discharge? Diarrhea? Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Sequel to delivery. Sex of calf..--l.“.emal-e ................... Normal? Normal Dead? Die? Date and cause of death. Post-partmient History of the Cow Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates illil C In?! 139 CHART fiM BDB OF. GROUP 3; B Study of Accessory Cotyledons. l. Apex of gravid horn. 2. Umbilical veins and arteries. 5. Apex of non-gravid horn. 4. Groups of accessories to be seen in apical ends of gravid and non- gravid horn. Negative animal. No history of calving difficulties. ‘7‘. ann162~H Bumd Name Reg. N 0. Date Born 5/1‘7/25 BREEDING RECORD Owner Date 1-4:CD Cow No.---M--BDB ...... Has animal been tested for infectious abortion? I)ate Reaction N e Ca ti V e BREEDING HISTORY Par. No. Heat Dates Dates Bred Sire Par. Date Ges. inDays Sex of Calf Sequel of Parturition ___n_§____ 1 1 10/10/24 11/19/24 7/15/25 266 Normal 2 11/1/25 7/3/26 264 Cleaned. Normal Weak calf. Calf died 3 mos. later of Pneumonia. Abbtevlafions: PM. mans Ges. m Parturition or calvmg. gestation or number of days carrying calf. Inad. Name. Reg. N 0. Date Born. 5/1 7/25 Age to Date. 141‘ BREEDING RECORD Cow NOMBDB Owner. Date. Has animal been tested for infectious abortion? Date ........................................ Reaction--- .Nfi éfitifilfi} ........ PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Heat Dates. Parturition N o ........ 2 Fetal MembraneS Sequel to Parturiti 011. Dates Bred. 11/1/25 Date. 7/5/26 Gestation in days. 264 Hours before passing?--...-..-....-8--h.p-g-,--.-.-... 'Aid? Treatment? Condition? Disposal? Normal? Vaginal discharge? Diarrhea? Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Normal? Dead? Die? Date and cause of death. D1 ed at 82 days of age. Pneumonia. Post-parturient History of the Cow Post-parturient Heat Dates Post—parturient Service Dates 'v til-\dll i.“ 3.0: nt‘ .Dix ‘11‘ v .0 i . v . vtl shit)! i .L CHART # AEB OF__ GROUP l B Study of Accessory Cotyledons. 1. Apex of gravid horn. 2. Umbilical veins and arteries. 3. Apex of non-gravid horn. 4. Accessory cotyledons. No history of abortion or disease. ‘1 Form 162‘}; Breed Name Reg. N 0. Date Born 4/2 2/22 BREEDING RECORD Owner Date Cow No..--AEB ............ Has animal been tested for infectious abortion? Date Reaction N e 83 t1 ve BREEDING HISTORY Par. No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition \ 4 1 9/11/25 '7/24/24 259 Femal e 0 .K. (1300) 2 Di ed l2/50/24Pneumon 8/18/24 5/19/25 275 Mal e 0 .K. 9/13/24 Died '7/20/25Pneumon 12/12/24 5 9/17/25 10/9/25 '7/10/25 275 Female 0 .K. 4 9/26/26 ___.._—\ Abhflfions: I’Giim parturition or calving. gestation or number of days carrying calf. . .1. >“-‘-"‘<--.-v‘" - .-.— 1'45 BREEDING RECORD Breed. Name. Reg. No. Date Born. 4/2 53/22 AgetoDate. Cow NOAEB Owner. Date. Has animal been tested for infectious abortion? Date ........................................ RCaCt-lon--N€,6&.t.i..VQ .......... PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Fetal Membranes Placenta 1 Chart made lesion me 11 {excellent condition. -, arid recorded as non- bativeo Sequel to Parturiti 011. Heat Dates. Dates Bred. 9/17/25 10/9/25 Date. 7/ l 0/ 26 Gestation in days. 275 Hours before passing?..................-.....N9.pmal ................... Aid? Treatment? Condition? Disposal? Normal? N o rma l Vaginal discharge? Diarrhea? Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Sequel to delinry s ....................... Normal? Norma 1 Dead? ‘ Die? Date and cause of death. Post-parturient History of the Cow rt'1011 Date. Post-parturient Heat Dates Post-parturient Service Dates 9/25/25 ‘9. .... JD cl; ’7‘ J .‘OOIOIOI 1““ I 1“ DC... “...K “00.00 0 J.- ... ..vl .. o'...‘e- an. I I... o a. . ..tooc al.h\\ 9K .. .j '05. .O C... .. .o’ to'oi ..e ltt :0. ..r ... .O’l I 'i‘n. I... [NI-I} i 3% J! u}. l l i |,| ' ‘ it? t I r. I ;..n 'w ll: I I 1‘. I! I ll ‘ i I l I i CHART # ABC OE_ GROUP 1 B Study of Accessory Cotyledons. l. Apex of gravid horn. 2. Umbilical veins and arteries. 5. Apex of non-gravid horn. 4. Accessories. 5. Denser group of accessories. 6. Still denser group of accessories. No history of abortion or disease. 141': 'va—r'i' """" “ ~ Form 162—H BREEDING RECORD Cow No.----A.EC ........... Breed Owner Name Date Reg. N 0. Has animal been tested for infectious abortion? Date Born 5/ 5/ 22 Date Reaction Ne 5a t1 ve BREEDING HISTORY Par. No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 9/15/25 5/15/24 275 Male Normal (Exp. born) 2 5/19/24 5/15/25 275 Female Normal #BAI 5 5/20/25 5/21/25 275 Male Normal Vealed 4 7/5/25 7/24/25 5/28/25 9/21/25 10/11/2e Abbreviation: Par. means parturition or calving. Ges. means gestation or number of days carrying calf. (chi .a.. ..irnar 0 '0 ”V... '0 II“. A, no. BREEDING RECORD Cow No.------AEC.... T49 Breed. Owner. Name. Date. Reg. N 0. Has animal been tested for infectious abortion? Date Born. 5 / 5 / 2 2 Age to Date. Date ........................................ Reaction- -- -N egatixew... PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. ' Heat Dates. Dates Bred. 53/20/25 Parturition No. 3 Date. 55/21/25 Fetal Membranes. Chfl P t mad 0 o Non-lesion negative. Many accessories. Few regulars Sequel to Parturition. Sequel to delivery. Sex of calf ........... Male ................. Gestation in days. 2'75 Hours before passing?-.--....N 01338.1 ............................... 'Aid? Treatment? Condition? EXC 51 1 en t; Disposal? Normal? Nor ma]. Vaginal discharge? Diarrhea? Mastitis? Milk-f ever? Pneumonia? Septicemia? History of the Calf Normal? N OI‘ma 1 Dead? ‘ Die? Date and cause of death. Vealed Post-parturient History of the Cow Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates 5/21/25 7/5/25 7/24/25 8/28/26 9/21/25 10/ 11/26 CHART # HBG OF GROUP 3; B Study of Accessory cotyledons. 1. 2. 5. 4. 5. 6. This photograph illustrates accessory cotyledons in the cervical region of a normal fetal membrane. Membranes are from a first calf heifer. Parturition was normal. The calf lived. These accessory structures could not have been due to disease of prior parturition. ’ Accessory cotyledons Normal cotyledons Umbilical veins and arteries. Gravid horn. nest of horn normal and not included in the photograph. Apex of the non-gravid horn. Parturient rent. 151 PAliT C CF _ GROUP 1 PIN-1T C Study of presence of naked areas upon chorion. . Ii:-I career # AGC OF_ cueu P l. C St11C1y of the presence of naked areas upon th e chorion . 3.. Apex of gravid horn. 22. Umbilical veins and arteries. :3. Apex of non-gravid horn. 4. Naked chorion of non-grrvid horn. 5. Group of accessory cotyledons. 331113 animal had a dead calf at the prior IDElrturition. infine an mal is negative. tulmis parturition was normal in all respects. 290 interpret tle significance of a naked chorion 34m this case one has to depend upon fetal mem- Tbranes of future parturitions. 154 l." I ' 100 Form 162—H BREEDING RECORD Cow Nowfbfgc ............. Breed Owner Name Date Reg. N 0. Has animal been tested for infectious abortion? Date Born 4/8/25 Date Reaction N 8551 t i ve BREEDING HISTORY Par. No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 12/5 24 1/7 25 9/22/25 259 Female Dead at delivery 2 11/5/25 8/6/26 275 M91 e Normal 3 9/1/26 Abbrev'mtions: Par. means parturition or calving. Ges. means gestation or number of days carrying calf. BREEDING RECORD Breed. Name. Reg. No. Date Born. 4/8/23 Age to Date. Cow N 0..-..-AGC ....... Owner. Date. Has animal been tested for infectious abortion? 1.5H6 Date Reaction. .--liegat-i.v.e....-.. PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Parturition No- 2 Fetal Membranes. ert made Recorded 2 s a non-lesion negative Sequel to Parturition. Heat Dates. Dates Bred. 11/5/25 8/5/26 Gestation in days. 275 Date. Hours before passing?-......-.-.-Normal ......................... Aid? Treatment? Condition? Disposal? Normal? N 0 rum 1 Vaginal discharge? Diarrhea? Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Sequel to delivery. Male Sex of calf Normal? Norm a 1 Dead? Die? Date and cause of death. Post-parturient History of the Cow Parturition Date . Post-parturient Heat Dates Post-parturient Service Dates 8/6/25 9/1/26 157 . .... . .2,” - .lldi".\ltl‘t .1 Fri}. .. n. n. a . “.1010: r‘tH‘DHM M’Ifigw M540‘“... .. . Vt . 0 . Ill» i . . A . 1.. .w”\o.}la.u- ..Ill. . i Orl- CIumT ifHC CF ‘ GROUP '1' C Study of the presence of naked areas upon the chcrion. 1. Apex of gravid horn. 2. Umbilical veins and arteries. 5. Apex of non-gravid horn showing necrotic tip. 4. Naked area of non-gravid horn. This animal has had eight normal parturitions. See attached breeding record. - ,1 EDI-m 16241 “Pition or calving. tion or number of days carrying calf. l K S - BREEDHH§RECORD Cow No.-..hC. ................ Breed Owner Name Date 1238- N0. Has animal been tested for infectious abortion? Ute Born 12/28/16 Date Reaction PO st P8 r'turi ent ‘ suspecg BREEDING HISTORY Par.No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 4/22/18 2/2/19' 287 Female Normll #HH 2 5/19/19 1/21/20 7 Female Normal #IB ‘ 5 5/50/20 2/10/21 285 Male Normal 4 6/26/21 4/7/22 280 Male Normal 5 5/31/22 5/ll/25 285 Male Normal 5 6/l}/25 9/5 25 5/15/24 287 Female Died 5/29/24 7 7/2/24 8/15/24 6/1/25 298 Male Normal E 8 5/24 25 8/5 25 10 21/25 8/15/28 297 Male Normal 9 10/15/26 x I] Amn'e'ififioms _d Par. means it ...? - 160 BREEDING RECORD Cow NoHC Breed. Owner. Bane. Date. RE N0. Has animal been tested for infectious abortion? Ilate Born. 12/28/16 AS? t0 Date. Date ........................................ Reaction.-BosL-partturi en t suspect PRESENT BREEDING RECORD Beat and Breeding dates for the last pregnancy. Heat Dates. Parturition No ........ 8 Dates Bred.6/34/‘35 53/5/25 10/31/25 Date. 8/15/26 Fetal Membranes. SeClue] to Parturition. Mmmmmm Gestation in days. 297 Hours before passing?-.---.--.-liomal Aid? Treatment? Condition? Disposal? Normal? Normal Vaginal discharge? Diarrhea? Mastitis? Milk-f ever? Pneumonia? Septicemia? History of the Calf Normal? N 0 ma 1 Dead? Die? Date and cause of death. Post-parturient History of the Cow Part . . ”- wk, Post-parturient Heat Dates Post-parturient Service Dates 8/13/26 10/15/26 \ I'm-m 161-11 BREEDING RECORD WITH REFERENCE TO INFECTIOUS ABORTION Cow N o ..... HQ ............... / AGGLUTINATION TEST . Isolation of -- - DATE Blood Milk 001mm Bact. abortus Heat Dates Dates Bred Par. Date REMARKS \ ¥ fi ~11". ----.- l 9 Apr. ----- I 6 may ---- c. ’ I t. July ...-u.— I ’ 4 5 Aug. an.-- . . 8/13/2t3 Eighth pa r- ! 1turltion. 297 4 ‘day ges. ,9 5 Oct . --.. ' ~~ . Milk plated magative . Quick test for * Oct. on the f» ‘ blood we 3 TT--- j'flov. -.-__ f Dec . g-.‘~ l Jan . ___- 1 Feb 0 --.- ‘ I u 1.; ,. L . vn _—\ Abbrefiafions. \ P _ ' . . lLi !.ti All! g! \. fl a- ll .L‘Il a» rem-” ,J 0- L \I o) ". emu-.13 ,4; HBG OF_ GROUP A C Study of the presence of naked areas upon the chorion. l. Gravid horn towards the apical end. Balance of apical end, normal but not included in photOgranh. 2. Umbilical vein and artery. 3. Non-gravid horn. E 4. A large naked area in the cervical region of gravid and non-gravid horn. Membrane from first calf heifer. Normal rar- turition and sequel. L- fl! ‘ ..r in. . .7. A 3 .A A \r ‘1 o. A .... C J n. \ 3 0, ... a PART A OF GROUP E PART A Fetal membranes from cows with history of disease prior to last parturition. u . ,fi‘. ..- \vs.‘ . . '1. 31‘ &) ensue # AFFE or GROUP _II A ' . ‘ Fetal membranes from cows with history of disease prior to parturition. l. Apex of gravld horn. 2. 3. 4. 5. 6. 7. Umbilical Veins and arteries. Apex of non-gravid horn. Diffuse placental area. Accessory cotyledons. . fi Dense groups of accessories. ii? Diffuse placental area, only less extreme than number four. The circular red areas are normal placental structures. No necrosis is present. [- it}! Kl Form 162—H Brood Name Reg. N o. IMWBWD 10 yrs. of age Owner Date BREEDHK§RECORD 167 Cow No. AFFE Has animal been tested for infectious abortion? Date BREEDING HISTORY Reaction P0 si ti ve . Par.No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 3 7/27/21 5 2/26/25 MMmmfimm: Par. means parturition or calving. 5/17/20 7/4/21 12/28/21 11/17/22 12/15/23 5/50/25 9/1/26 Ga. means gestation or number of days carrying calf. Female Strong Female Strong Female N o rma l N O rma l Aborted 7 mo. fetus(aborted) Retained Placenta Metritis-Manual re- moval of cotyledons maternal and fetal. Fetus, placenta and milk showed B.abort-_ v.30 Aborted. 8% mo. fetus Retained placenta, removed manually 12/15/23. Severe metritis. Metritis continued long while. Cleaned Normal Noticed a few large placenta 6 to 8 in. in diameter. Non-gravid horn has diffuse apex. Placenta neg. to B. abortus. Chart made of placena ta. No B, abortus in placenta. Abortus isolated Iron milk. .‘ .. _W‘U‘. .. 169 CHART 7% GE OF amour 1': A Fetal membranes from cows with history of disease prior to parturition. l. Apex of gravid horn. 2. Umbilical veins and arteries. 3. Apex of non-gravid horn. 4. Two rows of dense groups of accessory cotyledons. 5. Semi-diffuse grouping of accessory cotyledons. 6. Accessory cotyledons are to be noted throughout the gravid horn. The real significance of these accessories cannot be determined until 6 nsecutive placentas have been studied. 17o Form 162—H BREEDING RECORD Cow Now-”GTE ............. Breed Owner Name Date Reg. No. Has animal been tested for infectious abortion? Date Born 4/28/21 Date Reaction pc si ti Ve BREEDING HISTORY Par. No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 11/10/22 5/20/25 192 Male Deadat delivery 2 7/24/25 8/14/25 9/25/25 6/25/24 274 Female #AIG 3 8/6/24 5/19/25 287 Male Normal Vealed 4 8/24/2’5 9/29/25 . 11/21/25 7/12/26 279 Femal e Normal Died 83/17/26 5 9/28 26 1/2 27 2/5/27 Abbreviations: Par. means parturition or calving. Ges. means gestation or number of days carrying calf. Breed. Name. Reg. N 0. Date Born. Age to Date. 4/28/21 BREEDING RECORD Owner. Date. 171‘ Cow No...-....GE .......... Has animal been test-ed for infectious abortion? Date Reaction-.-."ID-0-31.3116. ........ PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Heat Dates. Parturition No. 4 Fetal Membranes. Sequel to Parturition. Sequel to delivery. Sexof calf Female ‘ Dates Bred. 8/24/25 9/29/25 11/21/25 Date. Gestation in days. 7/12/26 279 Hours before passing?.-.----..-..liar-11111 .............................. Aid? Treatment? Condition? Disposal? Normal? Vaginal discharge? Diarrhea? Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Normal? Dead? Die? Normal Date and cause of death. D1 C (1 8/17/26 Post-parturient History of the Cow Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates '7/12/26 9/28/26 1/2/27 2/5/27 PART B OF GROUP E PART B Lesions seen in normal fetal membranes. CHART # BOH OF GROUP _I_T_ B Lesions seen in normal fetal membranes. 1. Apex of non-gravid horn. 2. Umbilical Veins and arteries. 5. Apex of gravid born. 4. Group of necrotic cotyledons. 5. Cotyledons with calcareous villi. The cow is negative to agglutination test. The subcutaneous inoculation of guinea pigs with a saline suspension of ground cotyledons was negative to B.abortus. 1&4 Form 162—11 Breed Name Reg. N 0. Imam 10/14/24 175 BREEDING RECORD Cow No...-BQH ............ Owner Date Has animal been tested for infectious abortion? Date Reaction N 5 ga 13 i V e BREEDING HISTORY /_,__i fflLNO- Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition / \ 1 2/19/26 11/25/26 2'77 Female Normal K“ Abbreviations: Par. means parturition or calving- Ges. means gestation or number of days carrying calf. ...i._.""V-.‘ BREEDING RECORD Cow N030H Breed. Owner. Name. Date. Reg. F—o. Has animal been tested for infectious abortion? DateB—om. 10/1 4: /24 Age to :Date. Date Ten Te 3 i: s Reaction...-l§le€;s-tivc ........ PRESENT BREEDING RECORD Bettfij/ Breeding dates for the last pregnancy. Heat Dates. Dates Bred. 2/19/26 Partmit-iomNo --------- l ...................... Date. 11/25/26 Gestation in days. 277 Hours before passing?-.--_-.1.\lo.1.'.' U131 1'10 Aid? Fetal M e Inbranes. Treatment? Condition? Sec: Char t Disposal? Normal? N 0 rma 1 Vaginal discharge? 1) . . Diarrhea? Sequel “‘1 art untlon . Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf eliv Normal? N 0 mm 1 q \g\\/d ery_ Dead? “‘3‘ Die? Date and cause of death. male. .............. i Post-parturient History of the Cow ffltm'Itlon Date. Post-parturlent Heat Dates Post-parturient Serv1ce Dates ————-'—'/k - .....o-C'w- .( b‘ a' ll i. P! ' I v. -. Ill..'. -1 .r 0' . It. . . . all .. o‘ l I," c 2d- .4‘. ...' be” 00. r . . cram ..é BEG OF GROUP E B Lesions seen in normal fetal membranes. 1. Apex of non-gravid horn with large necrotic tip. 2. Umbilical veins and arteries. 3. Apex of gravid horn. 4. Calcareous villi of cotyledons. 178 \‘ i“- - r I ulszlxi l‘omzl Qg‘fi 1 7 9 BREEDING RECORD Cow No ...... M.-BEG.--- Breed Owner Name Date Reg. 1“ 0. Has animal been tested for infectious abortion? Date 50m 11 /1Q/25 Date Reaction Su spec t (See records in Group 911 of Serological tests. BREEDING HISTORY Pelt?) Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition .__/T W 1 2/5/25 11/6/25 276 Female Strong calf. Passed placenta. 2 2/7/26 11/20/26 286 Male Normal 1- " "fi-p. J'.l{'i:l“‘m g. V A Aumnr‘mmmm P‘r\ means parturition or calving. ”Jeans gestation or number of days carrying calf. PART C OF GROUP ‘E PART 0 Diseased fetal membranes. I (3" ‘de ._ CHART ”a ABI OF GROUP _T_I_ C Study of diseased fetal membranes. Classification. Five plus reactor fifteen days prior to the abortion. 1. Apex of gravid horn showing necrotic tip. 2. Umbilical vein and artery of gravid horn. 5. Cervical region of gravid horn. 4. Group of necrotic cotyledons. 5. Peripheral necrosis of cotyledon. 6. Peripheral and intracotyledonary necrosis of cotyledons. 7. Diffuse placental area. No necrosis. Note absence of interplacental lesions. 182 v- .. Breed Name—- Reg. E0. Datejom 9/21/22 BREEDING RECORD Owner Date 100 Cow No..-AEI ............. Has animal been tested for infectious abortion? IMWe ll/l/é6 BREEDING HISTORY Reaction mete-x- } o 5; i t i 1; e (Neg. previous 9 mos.) ELF—"40‘ Heat. Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition ___/A'— _'—\ #___- 1 4/15/24 6/22/24 11/19/24 12/5 24’ 9/7/25 277 Female Normal 2 12 7/25 2 18/26 11/15/26 270 Normal fl Abbzeviations: PM. means parturition or calving. 3. means gestation or number of days carrying calf. Breed. Name. Reg. No. Date Born. 9/21/22 Age to Date. BREEDING RECORD Owner. Date. Has animal been tested for infectious abortion? Date ........................ PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Heat Dates. Parturition No- 1 Fetal Membranes. Sequel to Parturition. Sequel to delivery. Sex of calf Femal g x k Parturition Date. ¥ ................ Reaction-..---Egg'elfiiflfiuuu ‘ ' Cow No ..... A El .......... Dates Bred4/15/24 6/22/24 11/10/24 12/4/24 Date. Gestation in days. 99/7/25 277 Hours before passing?---.-....l‘.lo.pmal ........................ . ........ Aid? Treatment? Condition? Disposal? Normal? Vaginal discharge? Diarrhea? Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Normal? Dead? Die? Normal Normal Date and cause of death. Post-parturient History of the Cow Post-parturient Heat Dates Post-parturient Service Dates 9/7/25 177/: F7: 2 18/26 BREEDING RECORD Cow No.......AEI ......... Breed. Owner. Name. Date. Reg. No. Has animal been tested for infectious abortion? Date Born. 9/21/22 Age to Date. Date ....... 1111/26 ............. Reaction-..‘l?:35‘.'?i‘.'3.'~::3$‘.-.15.0.8.1; i V 6 (Neg. previous to 9 mos.l PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Heat Dates. Dates Bred. 12/7/25 2/18/26 Parturition No. 2 . Date. 11/15/2 6 Gestation in days. 270 Hours before passing?-."IiiQ-I?YLl§.3.—...p§.§-§§;c§Q. ................. Aid? ? . Fetal Membranes. Treatment. 110 tree m ent Chart made and recorded as a Condition? lesion reaction. . Disposal? Normal? Normal Vaginal discharge? Diarrhea? Sequel to Parturition. Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Normal? . m r SBQuel to delivery. Dead? 14 o r. .... 1 Die? Date and cause of death. Sex of calf ......... Eemale .............. Post-partlrient History of the Cow Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates Open 186 .. ...l U- .q-u" up, Form 161—H BREEDING RECORD WITH REFERENCE TO INFECTIOUS ABORTION . Cow No.-AEI .............. AGGLUTINATION TEST - . DATE . gfiiagggrzis Heat Dates Dates Bred Par. Date REMARKS JO 0 5‘ Blood Milk Colostrum *— lrlar'. "'""""" 1 ‘ 2 3 4 Apr. """-- 1 2 5 4 Liay ----- 1 2 3 4 June ----- 1 2 5 4 July ----- l . 2 i 5 4 Aug. ----- 1 2 5 5 4 SCpto --""- 1 2 5 4 OCto -"“-- l 2 f5 4 Nov. aes-x-ee-x- 1 **p—- Abortus isolated 11/15/23". Normal calf 2 asp-- from fetal mem- 'Placenta chart- 5 **%*- branes. ed. 4 pp--- Colostrum plates Dee. *té-lt'lé'li' 1 neg. to B.:.bq:*t1.es 2 5 4 1927 Ja . «sex-eac- 1 2 3 l 4 1 Feb. -)HH:--l:-* 1 l 2 3 4 \_ H11» ‘ ’ ~:_..lsir_.*§lorrey Abbreviations: "' Par. means parturition or calving. ‘7 orm 160—11 BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1927 . m 1 (V ‘ I ‘ T ‘. 7 ‘ T III-(‘1‘ 1 , ' \ Date lm-n ‘ 14 IRS l. (1 lhi\l‘1R;\ l l( ).\ Nof March April May J unc July August September October November December January February I ,2 __ l l (f) 1"" z! (. l ' .H.'r . ' . . ’ ‘x I J/é~ i ’ ..I. i :3. .__; .4. an as: no ~I M er c-o :3 an :0 a :1 ..- _ ... ... ... ... .. w _ - _________ -- a— - .- «n - — - ~ ~ '33.):79'4‘" ’X‘ 9."): 'k'X‘ "X". 'X'Vi'ei' ':(":;";\";<";;" l 31.x «( )X 1) (”xx 1.: l{.\'l‘l( lN 1926 SECOND GENERA'I‘ION 1927 . 9fl§pllng 0f .__» —_ . l IIerd I W W , 011M, Par. N0_ Dale l’mru Days 111 171mm Sire \ Sex Remarks \90 March April l\l:1.y June July August September October November December January February l as: l i i I ‘ ”-3.9-..Lu- ~m~\-¢- ————— -a-o‘cuuo ~~~~~~ ..--Inna. ...-.5..." ...-.4qu ..--...” /r‘~1 “ - i (’ 4 - ... .. {fish/ill , me i g— . 1 .- l I «L .1 i 1.31 l “‘31 l i 1 l ' l . l I g l i l i I ”I” A l-LLL;_oi/_§:«;‘e _L 21L." __ l__ _ 1- _ _11 Ex e I 7 ; i 1. ; 1W1 ’ l _ __ a = ‘ t ' ; l l i l 1 i 7 ‘ : l = l 1 l ‘ i i : ‘ l l l 1 j . l y l ; l l lv 1 I: i l i i i‘ A 1 l 1 i A i) ‘ l 1 \ -_' l ;__ __ A; _ J. J _ l l t 1 7 l t ' i _ , I '—'_ T U" l ; ! l ' 1 * s. ‘ 1 . l l i . 1 » l l ik- __ _rl. _ l I l _ . _ l i ' i —— — — __— , » 3‘ i s l l l . l l I i . NE __ I , _ _ '_ 7_.l__.___._.k_ _' fl _ ___. ___‘___ I _— _é _— i \— -‘__ 1 l h ‘7 _ my I l l {x I 1 , ! i i _ “‘ - —~——»- .... ' I K A . k ar, means parturition. CHART # BAG or GROUP E 0 Study of diseased fetal membranes. 1. Apex of non-gravid horn. Umbilical veins and arteries. Apex of gravid horn. Yellow color signifies necrosis. Extent and location of necrosis is indicated on the chart. White color indicates the leathery thick- ening of the interplafiental area described by William and Hagen. 4 Lesions confined to gravid horn.~ Mo 8 t lesions are apical. These lesions Could not be due to cornual in- fection from a prior parturition as this is a heifer with first calf. The naked non-gravid apex with accessories cannot indicate prior disease for the same reason. _. M L ...-*‘Iu-a‘ Form 162—11 Breed Name Reg. N 0. Date Born BREEDING RECORD Cow No...--.I.3.!.‘I.Q ............ Owner Date Has animal been tested for infectious abortion? Date ReactionNeg. until Dec. P03. in Dec. 3: Jan. BREEDING HISTORY Par. No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 5/10/26 1/10/27 245 Female Aborted Abbreviations: Par. means parturition or calving. Gm. means gestation or number of days carrying calf. , -_.-"""§‘_ BREEDING RECORD Cow N0.....B.A.Q........... Breed. Owner. Name. Date. Reg. No. Has animal been tested for infectious abortion? Date Born. Age to Date. ' Date "Reaction“-.Neg ....until..De c . P080 in. Dee. & Jana PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Heat Dates. Dates Bred. 5/10/26 Parturition No- 1 . Date. 1/10/27 Gestation in days. 245 Hours before passing?-.----l\.:.Q.1f‘.1Tlal ' Aid? Fetal Membranes. Treatment? Condition? Necrotic apical Chart made gravid horn. Disposal? Le sion res. c tor . Normal? Vaginal discharge? Diarrhea? Sequel to Parturition. Mastitis? Do 9 s no t gi v e any mi 1k . Milk-fever? Pneumonia? Septicemia? History of the Calf Normal? Calf dead at delivery Sequel to delivery. Dead? Die? Date and cause of death. Sex of calf ............. female ......... Post-parturient History of the Cow Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates ...- _ "ms. 1/13/2'7 l-écc emulsion Plated lung liver abomasal fluid of Calf. 192 Form lGl—H BREEDING RECORD WITH REFERENCE TO INFECTIOUS ABORTION ,, , Cow No ..... BAC ............ AGGLUTINATION TEST Isolation of DATE Blood Milk C 1 Ba ct. ab ortu 3 Heat Dates Dates Brod Par. Date REMARKS .1925. o ostrum fl MRI". -'“""" 1 2 3 4 Apr. ---“- 1 2 5 4 may -..--.. 1 5/10/26» 2 3 4 June ~----- 1 2 3 4 July ----- 1 2 . 3 j 4 1 Aug. ...-D“ 1 ' 2 3 s i 4 1 Sept . ----.. 1 1 2 J ‘ 5 l 4 i Oct. ----- 1 , 2 . 5 4 Nov. ----- 1 2 3 4 Dec . ee-iepT‘I’ l 2 3 4 1927 Jim: ***** l B.abortus isolated 1/10/2'17 245 day dead 2 from fetus and fetus. 5 fetal membranes. 4 Killed 51 s a no -milker 1 20/27 Abbreviations: Par. means parturition or calving. l __ .MM.‘ CHANT #M BAF OF GMCUP I: C Study of diseased fetal membranes. Classification. Five plus reactor on July 1, 1926. Aborted August 10th, 1926. A period of forty-one days. Has remained five plus ever since. .1. Apical end of gravid horn trampled and missing. 2. Umbilical veins and arteries. 5. Apical end of non-gravid horn trampled and missing. 4. Yellow color indicates extent and location of necrosis. 5. Interplacental thickening of dfifrion as des- cribed by Williams and Hagen. The lesions seen here are nearer the cervical end of the horns. There is no necrosis toward the apical ends of the horns. Form 162—11 ' BREEDING RECORD Cow No ....... I *1--BAF--.. Breed Owner Name Date Reg. N 0. Has animal been tested for infectious abortion? Date Born 8 / 1 6 / 22 Date Reaction PO 3 1 t 1 vc BREEDING HISTORY Par.No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 11/25/25 8/19/24 268 Normal 2 11/25/ 24 8/28/25 278 Normal 3 11/27/25 8/10/26 256 Aborted Kmehflmm: Par. means parturition or calving. Ges. means gestation or number of days carrying calf. Calf died at birth. Cleaned in 8 hrs. . . I L ,- BREEDING RECORD f; . l ' Tl y . . r 3 s , -, . t ' Cow No..-M--BAE ....... l A. : l“ Breed. Owner. l ' l = Name. Date. A . Reg. No. Has animal been tested for infectious abortion? l ‘ i A Date Born. 3/15/22 ‘ 4 . 1 1 Age to Date. Date Reaction-....P-gsi.t.1.vg ....... T l PRESENT BREEDING RECORD ,4 _r ‘: - Heat and Breeding dates for the last pregnancy. Heat Dates. , 1: Dates Bred. 11/27/25 1 l Parturition No 3 . Date. 8/10/26 A ' ' Gestation in days. 256 Hours before passing? .......... Burma- ................................ Aid? ‘5 Fetal Membranes. Treatment? . ‘ ‘ ‘ Chart mad e Condition? N cc r0 ti c ,; ‘f -. - z ' 4 Note . Apex of eaCh' horn Disposal? '. is mi 5 singg'l‘rampl ed . g .- Normal? Vaginal discharge? l . Diarrhea? l l Sequel to Parturition. Mastitis? ‘ 1 Milk-fever? - Pneumonia? Septicemia? History of the Calf '; " i Normal? i ‘ l ' Secuel to delivery. Dead? 5 Die? Date and cause of death. Dead at delivery. Sex of calf Post-parturient History of the Cow ‘ . Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates ‘ ‘ ‘ ‘ s: .. l ~ V 13‘ Form l6l—H BREEDING RECORD WITH REFERENCE To INFECTIOUS ABORTION - Cow No..--M--BA.F ...... AGGLUTINATION TEST . DATE _ 31:2?232558 Heat Dates Dates Bred Par. Date REMARKS ] o 2 4 Blood Milk Colostrum fl tier . ----- May ----- Aug. ----- 8/19/24 268 day normal Sept ----- parturition . Nov. ----- 11/25/24 1925 Jan 0 ----"0 ‘iar 0 ”'“fi- May --—-- July ----- Aug. ..---- 8/28/25 278 day normal Sept t 1. ----- parturition Nov. ----..- 11/27/25 1926 Jan. -..--.. Feb . --~-- l Mar. «..-- 1 Apr . ----- 1 June ----- " July see-r.-- ! A11g. «wee-me I 30121 ted B .adortu 3 8/10/26, 256 day abort- from fetus and f ion. . placenta ' bept. meme-x» Oct . sees—mt- Nov . meme-n Dec . see-rem:- 1927 Jan . ewe-2* Feb . Haas-x- \‘ fi_ .._i_ ___..__ Abbreviations: Par. means parturition or calving. .- _- _ - . 3.11.1.1!!! 4 I r t 1 . f: i u 5 i I -.. , i CthT j BOF l c9 ' GdCUP 11 C 1 4‘- Study of diseased fetal membranes. Classification. Two plus reactor on June 1, 1926. Aborted a living male fiept. 15th, 192‘. A period of 107 days during which she reacted. Animal has been a five plus reactor since aborting. l. Apex of non-gravid horn. 2. Umbilical Veins and arteries. 5. Apex of gravid horn. 4. Yellow color indicates necrosis and show the extent and location of the lesion. 5. White color indicates the interplaCQntal thickening described by hilliams and hagcn. 6. A normal cotyledon entirely surrounded by the interplacental thickening. The light placental infe01ion probably accounts for the living calf. Form 162—-H Breed Name Reg. N 0. Date Born 9/5/24 BREEDING RECORD Owner Date zuuTK Cow No..-B.QE ............. Has animal been tested for infectious abortion? I)ate BREEDING HISTORY Reaction Po 8 i t i ve M P .N . ar 0 Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 # . 1/7/26 9/15/26 252 male Living and active. 11/4/25 Ah\ 1,,“ Par. muons : Ges earls o . o 'mea partuntlon or calvmg. gestation or number of days carrying calf. L ‘5“. ‘. _" fl . -..“-‘fi‘. ..._ , ZUL BREEDING RECORD Cow No..---BOF .......... Breed. Owner. Name. Date. Reg. NO- Has animal been tested for infectious abortion? Date Born. 9 / 5 / 2 4 Age to Date. Date ...... -- Reaction.Positive-mum PRESENT BREEDING RECORD Heat and B ' reedmg dates for the last pregnancy. Heat Dates. Dates Bred. 1/7/26 Parturition No.-_. l ................ Date. 59/15/26 Gestation in days. 252 Hours before passing?-.----.1‘io rmal....pa-ssage ............. Aid? Feta] Me C min-an . Treatment? hart m ade, Condition? Extensive necrosis Hecopd e (i as a lesion reactor. Disposal? Normal? Vaginal discharge? 30131 e di sci;an e . 9Quel to Pa. Diarrhea? rt, rltlon ~Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf _ Sequel to d . Normal? Normal but under sized . “”813; Dead? Die? SeXof calf.“ 1;" Date and cause of death. Ki 116d 9 d6 ys 18 t er . “““ ‘lale \ Post-parturient History of the Cow Pa 1‘1; ~ . W1 Date. Post-parturient Heat Dates Post-parturient Service Dates 9/ l 5/26 11/4/26 \ _____________ u ...,‘VN-h .. .....i s.-- .1 5' 202 REMARKS A Killed 9/24/26 Form l6l—H BREEDING RECORD WITH REFERENCE TO INFECTIOUS ABORTION Cow No ..... .5 OF ........... AGGLUTINATION TEST . DATE Blood Milk Colostrum 131311121331: 8 Heat Dates Dates Bred Par. Date Mir 0 ""'""""' l 2 3 ‘4 Apr . ----- 1 2 3 4 May ----- l 2 3 4 June as»..-— l 2 3 4 July *m- 1 2 3 4 Aug . *éHK-p- 1 2 , 3 l 4 ; Aborted Sept. “Hi-46* 1 lB.abortus isolated 9/14/26 living male 2 from fetal mem- " 3 brane. ‘ Par. NO. 1 4 ‘ 252 day ges. Oct. .me-se-Ht- 1 ***%% l.plates p03. . 2 ***-- 2 o ' n Cg o l 3 ****p 3."" pos. 4 ----- 4. neg. liov. erases-rai- 1 . 2 3 4 Dec . *ez-ez-ee-u- 1 2 3 4 Jan . -X~-IHH':-K- 1 2 3 - 4 Feb . 4’:-:H:-'x-* 1 2 3 4 Abbreviations: Par. means parturition or calving. BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1997 H ‘ Date Rom EL FIRST (HQNIQRATION \ 12-72931 March April May J unc July August September October November December January February I I i l E Vii/i) ’7 If“? r‘ i ’\n 9?? ’8“)?- ':§'h ’0‘.“ 'X‘ a? ’XVX“'X* ‘X' 31*).L'36p ’X‘ec'. 7r“. .x 7‘ ei'ii’dx '2 ’2 ’n 'n": " ‘13.: '1 9.: ’i: ii"). ';\ ’1 'X‘ 'x": 't '31 'n 7%", ‘n "Ii-.X. ex" 5;; ’;\'";i";:‘ l l-J I _. | a. I l l 1 ‘w ‘ V ‘ ‘ V ‘ r \ ' " ‘V ' 1 T . r x T g)-.. SluCON l) (ilnbluliAl I().\ 1920 SECO). D GILT ERA 1.10). 19.4 ()fl‘sprino ——* ——— — ~ . h of 7 ., . I . "VNO. Par. No. Dfm‘ Born Days in U toro Sire Sex Remarks liegd March April | May J unc July August September October bovombor December January February ~¥ >_ _- V—w-‘Aii—‘h—Kii r_‘_— —7#—‘-—‘ A. I ; DD — m” | l I l l i I l i z , ' . I I I male I vealed I I I E I I I l I | . I i I i ' ‘ .. '1 " F) l ‘ l ,3, 9 L) i i 2‘. l l l f i i . I i V l ‘ l I i l I I I I i = I ' I . I I l C ' ‘ f k I l 1 ' , u L I ~ : ) :n__ni 2”? ' kale swornul ugla i I I DD I ‘ ‘ I l I 'I l ' I I l J’J’ IUII I I Ilr‘l\.’\‘ I.,r\/’ 11!, H 'II' \ I\ III ‘I ' i i : ... .. i - "‘ “ " -' n .r- - - {ct-‘fir‘k- ’0‘“, 'f p— ices-cram.- 1."): '2: 7."): trade-X"..- item-3.- 9: 33% 9i")??? *‘k‘tc'iv'i’c i I a. an - a on - — n i l I ’ I l l C) r. A r 1 I l ‘ »- 13.1 4 I /O- ’4 "711' I t‘ «foal c Inn“ ‘idl 802 i I 5 I l l . I f , i l ,. t l i ( I . ' . _ . ‘I I 13 11/3»; I :37? I 1.1...10 a; x io/fl/JR I I ‘ | l I I l l “___" “ ‘““”“ ' '”" I ll 1 \I\ ' ' —‘J‘ TM rr fienei°aiiionm-__m BOF --.. \Li 9 3.5/2.6 252 L- is a if. c 1r. __ K I . I l \ I E I I I ”A 'f' l I ~‘_L_-._ l - AbbreviatiOn . i . ar. means parturition. 0mm? f} AEA OF GROUP _II 6 Study of diseased fetal membranes. Classification. Positive during period of test which existed from March 1926 to Feb. 1927. Five blus re- actor during period. . Two previous parturitions were abortions with dead issue. ' l. Apex of gravid horn. 2. Umbilical veins and arteries. 5. Cervical end of gravid horn. 4. Yellow color indicates necrosis of plaCental areas. Extent and leestion are indicated. 5. Extensive thickening of interplacental area indicated by white color. The light placental infection probably accounts for the living eight month calf. Form 162—H Breed Name Reg. N 0. Date Born 12/6/22 BREEDING RECORD Ovmer Date Cow Nonnfififi ........... Has animal been tested for infectious abortion? Date BREEDING HISTORY Reaction PO 5 1 t1 vc Par. No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 8/60/26 5/22/24 266 Male Dead at delivery 2 7/26/24 4/10/24 259 Male Dead at delivery 5 6/26/25 8/12/25 4/24/26 256 male Living Normal 4 7/30/26 8/26/26 9/16/26 11/8/26 Abbreviations: Par. means parturition or calving. Ges. means gestation or number of days carrying calf. BREEDING RECORD add. Name. main. Date Born. 2/6/22 Age to Date. Cow NOAEA Owner. Date. Has animal been tested for infectious abortion? Date ........................................ Reaction? .0. $3-1 tive PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Parturition No. 3 Fetal Membranes. Chart made . Recorded as lesion reactor Sequel to Parturition. Heat Dates. Dates Bred. 6/26/25 8/12/25 Date. 4/2 4 /2 5 Gestation in days. 2 56 Hours before passing? .......... Normal ................................ Aid? Treatment? Condition? Extensive necrosi s Disposal? Chg rt ed and burn Cd. Normal? N o rma l Vaginal discharge? Diarrhea? Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Sequel to delivery. Sex 0f calf ......... Male ................... Normal? 1.“ 0 rm a 1 Dead? Die? Date and cause of death. Post-parturient History of the Cow x.— Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates 4/24/26 '7/60/26 8/26/26 9/16/26 11 /8/26 209 Form 161—3 BREEDING RECORD WITH REFERENCE TO INFECTIOUS ABORTION COW No ...... AEA .......... AGGLUTINATION TEST I . DATE 301““ °f H tD t D Blood Milk Colostrum Bact. abortus ea :1 es ates Bred Par. Date REMARKS Mar. arms 1 H 2 3 4 Apr. *i'r-HM- 1 emf-H- Guinea pig (fetal 4/24/26 256 days 2 . f*f** inoculation membranes.) living male 5 *’W** positive. Chgrt node ° «Me-Hm I-Il . a . ,._. .. - a. 1 , ' “L , may *w'mfi. i ilk Ild COlC t1" lolljhu DODitch. of placenta 2 3 4 June mas-Isa- 1 2 3 l 4 July ss-zs-x-x-xs % 7/60/26 3 I 4 . Aug. it-IHHI-fi 1 fi/26/26 ' 2 . 3 4 1 Sept I was i 9/16/26 ' 3 4 I Oct . messes-rs 1 Q I 2 5 r 4 i hov . remem- 1 , 2 3 4 Dec. aux-I 4m 1 2 3 4 Jan. *-::-)’c** 1 2 5 4 Feb. asset-ms 1 2 5 4 1 ”4 'I {bbreviationm 1 .RF Par. means parturition or calving. } 2 . HR - 3 0 LF 4.1-. { LI: ...-2m 1 MM... omi 160—H BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1927 m)“ an FIRST GENERAT I ( )N I i931 M arch April May June July August September October November December January February {.1 (7 y. L .. l" 'I‘L _/_ 7 r ? I ' ' ' I ’ ’ \I ’ ' I , l .' .IV '.' 1‘ ' .2. L : $93,? p.- as '21")? p- a. I 6:")? p- a fi‘ecp- - dr'kp— 0" 9:53: pt- ’;€7\.7‘:‘p~ 6""?- u- '- ‘k'fi'fi‘i'p- di‘icw‘i'p .- ':€':f’X‘- -- ';\"X":€'pT I i I Sl'lC‘ON D (lliNl‘lRA’l‘lON 1926 SECOND GENERATION 1927 Cf OH§I)rillg 0f 1 I I I- 1101. 1 ___ fl—vw‘ — --r . i T J“ ‘ 0' P313 N0. DM“ “"1“ Days in U 1(‘1‘0 Sire Sex Remarks No( March April May J unc July August September October November December January February :1) H l l O ’ ‘ Q/ 41 -\ l. 1 — ... 1. I/. 1 I «:11 I Ii - r 7 ’ “ l 3 . ._ I .‘A/7 A /' l_ i'l ‘ I’ " 3.! or». I ADJ; i; l jJ‘ f“,‘.kgl_.}‘ \/ ' I). 5’, QKJ a - A1 ‘ l l l l ‘I ‘ \ ' I I I I l I i l I l l I I (fir-tr“ 'T. ‘.' .. . ‘1 ' .4 .. I 4 f I“ ll 13“} 15.1.3: “I\ I54 J- i l l ' '. I l ' I ‘r j l i l A . 'I I l .‘C.’~.‘l-J’—." ’r._\.'.,'“,.\r.ui I , ., ‘ ‘p v ; w J’..“'..". .L__1‘.\ :2; .\ _w_;; :5.» .‘ .3; \r _r’v~ .\;..u_.\4.'u"v_ u..\_u .\ \ t__'\'_\ .\I_ )1 1’). I§‘.\ \ \I_ I l l n n u n n n n n I l- %¢7:'n“,c',c 9C7: a. l\ n n n n“ n n n n n‘ A n l\ N‘n‘ n n n n n n n 7\ a 7. 'II‘ 0' 'N7\ I.‘ Is';. n*n"’\' n 6“ n n'1\ ‘ l . l 7 a - - .1 I f.‘ .... r. ‘ ' -,-».r " l ”V "1 ‘f" '4 ’ 'i E 3 ”5:15.116 ’ 3n 0 £31.14- j 11.143). I I | l . I l I I I' 'I T‘ ' . U “ "-\ ' Inez... 6 1.0122511. _7__ % l . I, agree-Cuzc- agun—c-s; I -cc-um -a-I-sf-‘N Can-t."— $$p-- -~--- -—-—~- ---—~— ~-_—— --.-...... --._..-r .1 . .. I e "Ts? .. , i - - - , . BOB I I. em... -1.": 1.0.7.0151 1 ,, _. _ _ _ . 3'1 ‘ ‘l J ‘ I v C. I 4..an mild f I . l 266‘. M5 -l I: Isn‘mnal __ #— 1‘ rc. Ges.-242112.15, i on ; H 07 3V) \‘l 5:“ HS l i I I0 C3 CD AEA Male Dead G. e11. 1. 2 V r - - W 259 Male Dead 53 Ciel \Lm/gggg 955 Male Tigers 1 came. 275 Male Normal LI: 0 IPJ \ A1lbw Viation: r ar. means parturition. 211 CHAMT #H CO A 0a; GNOUP.ll C Study of diseased fetal membranes. C assifieetion. Artifically infected by feeding Sept.7th, 1926. Reacted **p-~ Sept. 20th, 1926. hborted eidht month fetus Nov. 30th, 1926. 1. 2. 3. Note This the m “pex of gravid horn. Umbilical veins and arteries. Apex of non-gravid horn showing lere necrotic tip. Yellow color indicates extent and location of necrotic placental areas. White color indicates the interplacental thickening described by Williams and Hagen and others. apical necrosis of gravid horn placental arses. animal is also a first calf heifer and we know source of the infection. This could not L:ve been a cornual infection due to harbored organisms of infection. Form 162—H Breed Name Reg. No. Date Born Feb . 1925 BREEDING RECORD Owner Date 2:113 Cow No.---fl-..CC--.A--- Has animal been tested for infectious abortion? Date Reaction Po 8 i t 1'. V6 BREEDING HISTORY Par. No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 Unknown ll/tO/EG x Aborted. 8 mo . fetu S. Lived one hour. Passed placenta. Abbreviations: Par. means parturition or calving. Ges. means gestation or number of days carrying calf. 214 BREEDING RECORD Cow No.H--.CG.-A ...... Breed. Owner. Name. Date. Reg. NO- Has animal been tested for infectious abortion? Date Born. Feb. 1925 A89 to Date. Date ........................................ Reaction.--Bo.si.t.1.ve ......... PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Heat Dates. Dates Bred. Unkn o wn Parturition No.--_ J. . Date, Abort ed 11/ 50/ 26 Gestation in days. Hours before passing?-_.----.---.-_- Aid? Fetal Membranes. Treatment? Condition? Disposal? Normal? Vaginal discharge? Diarrhea? Sequel to Parturition. Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Normal? 8 mo . fetus . Lived one hour . Sequel to delivery. Dead? Die? Date and cause of death. Sex of calf ______ X Post-parturient History of the Cow \ \ Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates K 11/30/26 m -..-'VK ...- {0 15 Artificially in- fected by feed- culture :9 7/26.Heacted Par. means parturition or calving. Form 161—H BREEDING RECORD WITH REFERENCE TO INFECTIOUS ABORTION Cow No.-H.-.C_C,-.-A ..... AGGLUTINATION TEST ‘ - DATE gmiaugn ff Heat Dates Dates Bred Par. Date REMARKS Hm! mm cmmm aiamm liar o "' " "'"" Apr . --- -- Allg . nu- - I- .- Sept. sap-— :in 00 t . a: amt—4'. 9/20/26 ibv. .*n+%* .From fetal Membranes. 1.Lungs of fietus 2.Kidney J L . 5.Liver Ike. *aasa 4.Abomasa1 fluid Jan . «leases-I:- F cb . MHz-r a: .Mbmfimmmm _ A __.-o-"*.. ..- ."Hb‘ __ ’wr‘. Clh’iiil‘ if [IGA OF GROUP ‘_i'__1' C Study of diseased fetal membranes. Classification. I do not know how long this animal was infect- ed because she aborted fifteen days after the first test. The first test was five plus and this reaction has been constant for the year. 1. Apex of gravid horn. 2. Umbilical vein and arteries. 5. Cervical end of gravid horn. 4. Yellow color indicates extent and location of placental necrosis. 5. White color indicates internlacental thicken- ing of chorion as described by Lilliams and lkugen. 6. Petechial lesions similar to five. The extensive placental necrosis probably accounts for the dead fetus. *- .—___ _.._ not" 9 ‘1 ‘. ' l l ‘ Form lGZ—H H l 8 - BREEDING RECORD ' ‘ V \ COW No.---AGA ............ i ‘ ' . Breed Owner 2 S {I I Name Date Reg. N 0. Has animal been tested for infectious abortion? ; ‘ [ Date Born 2/7/23 Date Reaction P0 81 ti ve BREEDING HISTORY . l Par. No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 7/7/24. 4/15/25 285 Male Normal Sold 2 7/20/25 8/16/25 4/15/26 245 Female Dead at delivery Fetal pneumonia. 5 5/51/26 6/24/26 _. 8/15/26 f l l l ‘. ‘ l l 3 ; l l .__. l .MMnmwuhmu Par. means parturition or calving. Ges. means gestation or number of days carrying calL _ r -.— a. w -- “er-"r , “ t—‘W‘ ~-~ e- ---——- . w-i'V“, ' 9. 1 9 BREEDING RECORD Cow No....AGA ........... Breed. Owner. Name. Date. Reg. No. Has animal been tested for infectious abortion? Date Born. 2 / '7 / 23 Age to Date. Date Reaction--.1?9.§.1.t.i.V..§ ........... PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Heat Dates. Parturition N o- 2 Fetal Membranes. Chart made (Right horn) Recorded as an infected positive Sequel to Parturition. Sequel to delivery. Sex of calf ............... Egmale ........ Dates Bred. '7/20/26 8/ 16/25 Date. 4/15/26 Gestation in days. 2.43 Hours before passing?..-.--..'I'.-en hours Aid? Hetained non-gravid horn Treatment? 011.. and iodofor'm Condition? Badly diseased after-birth. Disposal? Normal? Vaginal discharge? Diarrhea? Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Normal? Dead at delivery Dead? Die? Date and cause of death. Post-parturient History of the Cow Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates 4/15/25 5/51/26 5/24 /2 6 8/15/26 < Form 161—H BREEDING RECORD WITH REFERENCE TO INFECTIOUS ABORTION Cow No..-AGA ............. AGGLUTINATION TEST . DATE Blood Milk Colostrum alfiai‘fil‘a’fis Heat Dates Dates Bred Par. Date REMARKS “‘1' o ”m 1 2 5 4 Apr. *H-K-tb :’ 1 B .abortus isolated 4/15/26 24's day 2 from fetus and fetal abortion. 5 membranes. Fetus dead 4 _at delivery May was 1 5/51/26 ' 2 5 4 June mate 1 6/24/26 2 5 4 July *Mfi-fi- “ 1 2 3 4 AUg . we“ % 8/15/26 3 l 4 . Sept . same-2H!» 1 . 2 : 3 4 00 ‘L . #4443“? 1 2 3 1 4 NO V o *fi‘lfii‘fi- 1 2 5,- 4 Dec . «em-r. 1 2 3 4 Jan . fiH-ééfi- ‘ 1 2 3 4 Feb . *-i:--x--2£% 1 2 5 4 \ Abbreviations: P - - ° W‘- lneans parturition or calvmg. ,mi, Form IGO—H BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1927 M \_._ _ \j hm. 1.3m.“ 191qu‘ (TNFRN’I‘IUV 12'“le March April May June July August September October November December] January I February ‘ "‘ “""“ l). ’e. w n l 1 3 5"" (3 13/31 ‘31 {J \I C d I l (J ) Milt “fies-'5‘ gamut-.- I _a--=.=’ -ggo-u- :_&-:_ .__-...... ~—--g&g¢- up-cvau-g h.) £KU6 L r\~/I‘ | SIX t()\'1_) (llfiNICRNl‘ION 192G SECOND GENERATION 1927 \ . Waring; of fixww , y . . , ‘ l ‘ I v r \ — . \ ‘ ‘ ‘ t v I (“WM 1).“. \*( l‘rtle l’mru Days in [item gm. gm. Reum'lts “Nd March April May June July August September October l\oumbe1 IIOPNDDOI January Febluaiy . k . - i. , ‘ ‘ l _ J _ k . No. I I M:- Q l . l | l I . . l 7 " f.. l . -' A; . . i I -,/‘\/"g"./m7 rad-3 ,l..‘ 7 .... 1 W“ ' 'fl *— -‘ -’ '5 " Lg“ :ALLRLJ G”. Al‘s“? 5‘] . CG 1 3H”; 'i l i I l f i l , «I ~ ' i y 'w w." .3 .‘ . .‘ ‘-'.\-.~ “.94-. . .m. . we- AL.‘,’..$.- .‘_.‘L.“. . , I .,'. .1 .\I \I - . Lp"~a . . - - ~p— - ‘ no 0 o - I I‘ ‘ ’\ I. lb ’\-- l l ’;\’3:‘ppp -).--)\"X"X‘.. l ~234HHC-p 64-1.» I n n 'n n';. n n 0% “”0 n n n I up n n n n n n n n n a n u . a t . pp \ 2 V (t) (’3‘, l/‘—>r": l {- fNr) t 3H3 MI" 1 ’1 T. . ~4' 1r 1 3 ‘7'. i ‘l W K’_/—{J"k,t-} 1 a3), ; ’ td-wtl. 19C m -. .445“ A. - z x- 1 v , I . J , I l i i ‘ l l I “any” i m-“ma ; _n-nu ~mw~°r= -mno . ’2 ’ / 3 . - 5 <1 ‘1 r ~. l . ‘; - ”i 9741 . U I 1’3“” ’4! a :Crtji) I f 3) g} Q A i'a‘r'ijj fl .4 stain: k : CG ? i . i l . i . . e , i I I l 4 i 1 {a} "‘1 ( , p/l/ ', F“ E (D m P“ I ‘. 7 .i 3 ‘ —. 7r -‘ f~ .1 ‘ l-i - {j l r, ‘1 r? /(N l ' www t. ‘ « Litil e itL_]_l_giL _4 4 a J;£_ . J. Ll». t,,_ i J} t ( _- l l 1 l ‘ I H. ’! 1' . a I k“. . -: . . AGA xx 4-1111 c. uenerat on , l l a l ./'l F’ m 'M RT - C: q ‘ -.,,-_ \‘l l _‘ 7‘ _ “ .. ‘3 Ralf; real, (5 normal Doll... I 4:3 Rennie-ls Ileana u. del. . » a F» D'l /... 5. ‘\ If) 1’5) {0 <1 f. ). ." \ _ '1 ale 1m r e 44- \ Abbre \ Viation : ar. means parturition. I?£rii3f I) OF ”1ch IT Study of fetal membrenos from cons:cutive parturitions. ‘1’\‘ PART D o F amour I}: Uharts of # AEO On Uhert AID parturition numb r three, the gravid horn has fifty-one Placental arvag an inch or more in diameter. The non-bravid horn has twenty-nine and 1 many accessories. The arex is naxed. On Uhart AEO parturition number four, LL re is forty- nine placental areas an inch or mOve in diameter. In the nor.1_-(-_-)ravid born there, is about tr-xenty and inch or more in diameter and an entirely different picture of accessory structures.- The apex s naked. C 1 iii; 1T ff n :30 OF JMCJD P I i. D (First of censecutive fetal mem- branes. Parturition number three.) Study of fetal membrcnes from consecutive parturitions . l. Apex of grcvid horn. 2. Umbilical veins and arteries. ‘. I" o. Cervical end of gravid horn. No record as to whether the gravifi horn w" I C-‘. the right or left. 8 223 E 1k (1114.1? ,4 .‘rr A-&Ltt~ H 51.4 c? O _ /'7) 7' lLCMP 11 D * (Firet of confidentive f'tal mem- branes. Parturition nunt r three.) Study of f tel membr n e from consecutive nertmritiens. l. Abex Cf nen-4revid hern. u‘ \- ‘ Acceseeriis [fl 0 o Dense Lgroup. of ecceee"ri~ Ch] 0 m 40 1473‘?sz (”Ft/fix. 27 .- I! r f as): O J .‘i ‘ if 41’. on J 6109.01.- 6.91; sh . I! It.‘ ',lll.4'!t luv..." . I}. II. 0mm 2,: 11:30 OF GROUP E D ‘ (Second of consecutive fetal mem- branes. Parturition number four.) Study of fetal membranes from consecutive parturitions. l. Apex of gravid horn. 2. Umbilical veins and arteries. 5. Apex of non-gravid horn. No record made as to whether the gravid horn was the ridht or the left. ‘JHU 230 Form 162—H BREEDING RECORD Cow No.---AEO ............. Breed Owner Name Date Reg. N 0. Has animal been tested for infectious abortion? Date Born 2/2/22 Date Reaction Po si t ive BREEDING HISTORY Par. No. Heat Dates Dates Brod Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 8/20/2‘" 5/28/24 221 x Dead at delivery 2 6/22/24 5/24/25 2763- Mel e Normal Sold 5 5/2 7/25 5/28/26 275 Lg. 1e Normal Sold 4 5/8/26 2/5/27 2'76 Female Normal Abbreviations: Par. means parturition or calving. Ges. means gestation or number of days carrying calf. ’ 4!, III! a «.1 y .p I x O I ‘1‘ lOr. 'I .. .... I .0 I l.|||’ ll ill! _ «lili- l! i] I} I! r I. Q. If BREEDING RECORD Breed. Name. Reg. No. Date Born. 2/2/22 Age to Date. Cow No....AEO ........... Owner. Date. Has animal been tested for infectious abortion? Date .-Reaction.-.. .. .20 Siting, ..... PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Parturition No- 15 Fetal Membranes. Chart mad e Cla ssifi ed a s non-1e sion reac tor Sequel to Parturition. Heat Dates. Dates Bred. 6/ 2 '7/ 25 Date. 3/ 2 e / 26 Gestation in days. 275 Hours before passing?-.-.-.liormal .................................... Aid? Treatment? Condition? Disposal? C ha r t ed a nd burned Normal? Ii 0 rma l Vaginal discharge? Diarrhea? Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Sequel to delivery. Sex of calf ........ MEL 1e Normal? 1‘? o 1" ma 1 Dead? Die? Date and cause of death. Post-parturient History of the Cow Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates 3/28/ 26 5/8/26 420B BREEDING RECORD Breed. Name. Reg. No. Date Born. 2/2/22 Age to Date. Cow NOAEO Owner. Date. Has animal been tested for infectious abortion? Date ......... Reactionf 0.3.1 .tilxic ........... PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Parturition No. 4 Fetal Membranes. Sequel to Parturition. Heat Dates. Dates Bred. 5 / 8/ 26 Date. 2: /8/27 Gestation in days. 2'76 Hours before passing?..-.-.llormal .................................... Aid? Treatment? Condition? Disposal? Normal? Vaginal discharge? Diarrhea? Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Sequel to delivery. Sex 0f calf ........ Female ............... Normal? Normal Dead? Die? Date and cause of death. Post-parturient History of the Cow Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates 2/8/27 Form 160—11 BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY .__...“ R “A ......“ ___.ufl— FIRST GENERATION fixmfi 77777 w “A“ I . ' " . ’ ' N... l \I IInqu‘ lI'ebruarv \ ' ‘ ’ ‘ " ‘ ' Herd ~ . " Ma ’ June Jul Aurrust September Octobel howmbm J((,(nl;)(1 ..1 . y ‘ \A Dale im'n FIRST Gluh ERA 1 [UN N0. 31.11911 Aplll 3 y b i | l .. .. , , .. u l : . I I x .‘ 1:00 ," ,"_'\_,“."~'. -' -- -‘- U . e- \' \- \I U 91“}. Jyou" __. “ JLgb‘I‘ .II.‘_.:J_- ;I\.4\_——- . . ,I ~\ I I“. t “‘1' VI. 4\ n I! n I I I -‘ u .. V U --_'~-_'\;_.\.. I. 1.- <.v_.u_7‘. .\_.I‘.-._,‘..‘. .-. ,~-~ c \ ‘ ‘ ‘ n n 1" I\ l A I 1:,- :2 "‘ ’ p 1wp .... n ,. ,. a p--- ”m .. .. .. I I I .. I I I I . pp l i \S‘L— -EA 1 ‘ ‘ 1 -1 1 r‘ v ()-v ‘ ' ‘ " ' 1926 SECOND G LNLRAJION 19hr SECOND (lla.\ln[i.\ l I()I\ . OHSIH‘iDQ of I i“ . . . ) . .1, , Herd 'M'u'eh April May June July August September October Novm'nber December January February (UWUO. Pa1th. 1 Dale Bum Days 111 Utero Sire MK “”1“” ‘5 N0. ‘ ‘ 1 A130 *1 ___ l 1 i ' l / . l r, j : \mmlxfig :___.. A L if 7 ll _ *1 J. 2 a '11—: _ f AB“ I ‘ l l : V l i 3 1 i l . : .. _ _ ‘ 5 1 i ZOE: l_ __ ,# h____. .Minfl-_ “ V ’ l'ei- l_-____,__1 _ ___. n.-_ __T_____._ ‘L I I i ‘ i 3’ E ' | l l i ‘ l ‘ 1 l I | l i j r 4’ ‘ 3 1:37;. ', -]LL‘ 1‘, ‘ LOT-"13.1 I! . - 1 _ , i . l ‘- E l . , 2 l I . ; g i ' r ‘ i. I T I : 11 276 is, ___tfemarle _fi~b_owrm_arl_ a 1 a; z 1 E l I . | i l, I 5 'g 2 \ l £— fl KER h _. .4 ___—_— , V l i \ ‘ ——4 fix 1: .f \ | RM 1 l _ _ w 1 _ - ,‘2 _ I 53 \M g /" \ l: .3 Abbr > - eVIatIOn: ar. m . . g, cans Dartumtlon. if PART D OF GROUP ”._E__I' “harts of.# AFB On Charts AFB All parturitions of this animal have bran I‘Lomal. The two consecutive parturitions I have recorded here are the third and tle fourth. Tie brevid horn of tLe third harturition hes seventy-seven placental arees. The non-grtvid horn has thirty-two plaCental are s and numerous access- ories. The :aternel mnnbranes of the fourth parturition has a 5reator number of plaCentel FPPES ard an en- tirely differevce ricture of accessories. The large necrotic tip is absent. 90" 9.35 \i: .. CfinuT j “EB (F amour II D (First of ecwxmmNJtive fetal mem- branes. Parturition number three.) Study of fetal membr;ges from consecutive parturitions. l..kxn:of orevhllmrn.‘ 2. Umbilical V f‘\ 31H 5nd artery. 03 O C :3 <1 H- o r. H CL 0 H3 gravid horn. -.. ' I .rr’.‘ ,f tut h‘ 2 3'7 I O .4 . t 2r". ‘ ‘0? . it, 55.2. 59......5 («or £k§tzbtk on. rilrgrxt r 649;} L r I; . Qu. ’5 . . y ‘ ”ivy- II: o :. _ Kw. - . . .‘ {fl uw-iukw... if’l‘filrwl‘tkt . a ‘.r(‘” rav 1M," - x ‘ ~ u CHan f AFB OP dhOUPiIz:D (First of consecutive fetal mem- branes. Parturition number three.) Study of fetal membranvs from consecutive parturitions. 1. Apex of non-bravid horn showing large necrotic tin. 2. Umbilical vein and artery. 3. Cervicxl end of non-brevid horn. 4. Accessory cotyledons. lg; 00. [- / 0‘ —od‘- 5.. b~‘ C“ $4 4 239' t o. . . . . . . . . , . . 1 é?w ‘ a. i if £.v. Egg. . ..l ..n. ‘34 3E35.'0 no ' ..ér gfbrvfr’ ‘55.“?de I .urgg‘ . L wit? . VI t‘gi _‘..Qf .oT-u‘vvnr...|.. r - 4. . . . q. - r. . .. . . . . . qt. Ibiza)! .l . . Id. .. . , , . y i . . CHANT f AFB u HCUF II D vc fetal nem- (Scccnui of C(w;sch, t four.) ti b a:nn3. Parturi ian nunh Study of fatal membr:ncs frov consecutive parturitions. 1. Apex of SPKViG Lorn. 2. Umbilical veins and arteries. 5. tripe}; 0f mwn-(‘J'rzAvid horn. um.“ - J A ~.- own N 0.41 Form l62—H BREEDING RECORD Cow No..--AF.B ............ Breed Owner Name Date Reg. N 0. Has animal been tested for infectious abortion? DateBorn 6/10/20 Date Reaction Positive BREEDING HISTORY Par. No. Heat Dates Dates Bred Sire Par. Date Ges. in Days Sex of Calf Sequel of Parturition 1 4/19/22 1/27/25 282 Male Normal Sold 2 7/20 25 8/6 25 8/25 25 9/8 25 9/26/25 10/31 25 12/7 25 12 27/25 5/15/24 12/26/24 279 Male Died 12/28/24 3 4/4/25 1/15/26 285 Female Normal #BCE 4 4/5/26 l/ll/2'7 282 Female Normal Abbreviations: Par. means parturition or calving. Ges. means gestation or number of days carrying calf. I . ,l‘llllll‘ I.. BREEDING RECORD Cow No..-AEB.......-.-.. Breed. Owner. Name. Date. Reg. No. Has animal been tested for infectious abortion? Date Born. 6/ 10/ 20 . Age to Date. Date ........................................ Reaction-.-P.O siting: ......... PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Heat Dates. Dates Bred. 4/4 /25 Parturition No. 5 . Date. 1/15/2 6 Gestation in days. 285 Hours before passing?-----.---..1hnce--bnur-a ................. Aid? Fetal Membranes. Chart made Non-lesion reactor Sequel to Parturition. Sequel to delivery. Sex of calf ....... .139.le ................ Treatment? Condition? Disposal? Normal? Vaginal discharge? Diarrhea? Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Normal? Dead? Die? Normal Normal Date and cause of death. Post-parturient History of the Cow Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates 1/15/26 BREEDING RECORD \ Cow N 0.....-AFB ......... Breed. Owner. 1 Name. Date. ‘ Reg. No. Has animal been tested for infectious abortion? 5 Date Born. 6/10/20 ‘ Age to Date. Date ........................................ Reaction-.PQg.i ting ........... PRESENT BREEDING RECORD ~ 9' Heat and Breeding dates for the last pregnancy. Heat Dates. Dates Bred. 4/5/26 Parturition No. 4 . Date. 1/1 1/27 Gestation in days. 282 Hours before passing?---.-.---llonual ................................. Aid? None ‘ Fetal Membranes. Treatment? 7; Chart made Condition? Exo ellent '. 4 Disposal? Normal? 1‘4 o rmal Vaginal discharge? Diarrhea? Sequel to Parturition. Mastitis? ; Mi1k_fever? Pa rturi ent pare si s ; Pneumonia? ‘ 7 Septicemia? ‘ History of the Calf Normal? NO I’Y‘la l Sequel to delivery. Dead? Die? , Date and cause of death. Sex of calf Perla 1e Post-parturient History of the Cow Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates 1 1/11/27 . I. l . .I I f ..Ibi'l'llll I I . 1 II I I‘ll. .rll'll“ v.0 . . I .Illii . ll IIIII orm160—H BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1927 E \ Date Born FIRST GENERATION 111$? March April May June July August September October November December January February X-__ __ __p _wm -m,___ I __ ___“ l a . 5 l . ; AFB *%%%% *%%%% i 65/10/530 assesses-:6 ewe-e454 'I'r'fi’ié’ii'fi' eras-x44»; '3'c'31'~""}H(° aeaxei- We“. flew” meat-sea- Meme-:1- 1 31.300“) (”QNIQRA’I‘ION 1926 SECOND GENERATION 1927 Off. ~' 0' ~,' E "I I" r ‘ _—_—fi “"7“— " . H '7 - "fl”— . T 'ow Ngéilmfiy \v” l Date Born llDavs in Utero Sire Sex Remarks §$éd March April l May J uno July August September October November December January February ‘ 'f_;_i . __ 1 ‘ __dgm ”l4.--— #F_g_ ___ .7 ,_ V _ ,_ ' g .l__, _ ,_, r ll,____-l - ,_ - l l AFB t . i l l ; 1' ; l l . l ... ‘ l - 4 ,1”- L&%&5llam. on”- than JLLK.&fldv .. _ _ ———F——m—~—fl—~Wu -— 4 {AFB , ! i l l 4 l l i l I l l " = l ~i-12/26/24e—2?9fl-a~um4—--leale~- Died~12/28/24 t "gnun4_—um——~u—~-_m~—'ar~——_~—~~~—w~~—~#———-~3~ ”We ; ' , i i g j i , i 1 , . . g AFB l L---’__l[.lfi/26 i 285 A _. Female[_Q,o_Ii_..o,___ll--_§ BCE -.- , ! AFB l ‘ g 1 l l i I i ' l l I :2: =7: ‘1» d". \b 1" -~~'.J -—~-— -~'-— ---—- ----- ‘3’" . _1/;1/27W1_282._; , V- -_ _ __ Female; 0. ...- ,_ _ 5-! — o—o———~-»——~-—~—-l Km.— \XJ_ Abbreviafi 011 : . 31‘. , means parturition. ~~ ..‘M _ -.. < r _ . 7 "’ ' .’ :1...) ’ p PART D OF GROUP 11; Charts of M BBG 245 z ‘2, .. 0*. \ l I l 3 I \ '3 11 'i D t N 4 i l‘ ‘ j ‘ t b E00 BBG A lieeord of t'. .o ConaeC'eive re turitions both known to save occurred in time linLt horn. luvs-e are the. secr‘r; and t 1rd p rturitions. J Parturition nu"b‘r t¢o was a two hundred 03C I‘ilty—s x d?y z-hortion.11.cwv we psssed ind reermmhwi. Uron ex- aeirvrtion incuticter'jj1e lelflfi horwrsts rqn . t co‘rwoln s a CJZM‘ were noted lw et 'eu Tunen ~nd horas. Theee Could be brocen deno 1’; ' - _0 ‘ ”- _' ,_ n‘ r ‘ ~15 ,~ r" 7- I‘he exteha1te necroe1s C1 3110 l r1?CCHtul area 01 Ar,tld horn is to be, note-cl. Tlrz‘re air-r: "1"‘tv-t11rie Pf‘,‘COT‘Clr‘:(‘l placen- tal arrgs 1n tle grsvid Earn =hd t lee wareoerfi d norwal ervw 9 1L the I‘tiIM1*W£WXP€ X oi‘tflvx:grevifi *(J”l‘fl 3135 a to 31 of sirty -tmo. The non-tflmvid Zorn his tV‘fity-PPVCH w '2 I/xvx '. (“1“: r1( ‘-,iLt.41 ‘. J -' S. The rVMRnwnrta of the tldxwl'rrrturition Ce tet 9 C)VJJ< Cr: (>f‘ t}, a (if 3 “.C13 i_HCKiC;a1"d it; 13 6 Cl *:“t 01' SECO'ICI T F’l‘iz'ltlul. 1:"«43‘ f disease 1n the apex ofi the The aeeeseeny s ructu3e in tie CerviCzl region ,enhot be a.ttrib.ted to Ciseaae 0f the prior pertfirition. ..., N- .v—fi ___—...— CanT ”-5111 LEG CF .‘ v \ cu..— JxéCL‘l" 11. D —-- (First of Consucutive fwtal mwm- 7‘ branes. :arturitinn number two.) fatal nenbr*nfis from consecutive C s Apex of gravid horn. Umbilical veins 2‘ 31d &!I"tfi‘1°i€s. Apax of nan-gravid horn. Yellow C0101 siQHifies decrosjs. Extent and 100 Lion of necros's is indicated on the Chart. White Color inmicates the l athery thick- ening of the im'; “pr.“ cemtz-l :rea described by Williams and 1431ch. . .__—A A..— Chnn'fl ”"le BEG (F mom? _Ij; D (Scccnui cf ccu1secwtfiwns fhts].rncw- brines. Parturition number t1ree.) Study of fetal membrrnfls from ccnsccutivc parturitions. l. U) s 93 |,_.1 H Apex of ncn-bravid horn. DMOVinQ ncCPCtiC tir. UNbiiijl Yflins and arteries, Apex of dravid horn. Nocrctic areas. Accessory cotyledons. BREEDING RECORD 251 Cow N0.L..BBG ..... Breed Owner Name Date Reg. No Has animal been tested for infectious abortion? Date Born 1 13/25/22 Date Reaction Lit 1‘0 "1U SL1 s :3 EC t to Positive BREEDING HISTORY Par. No. Heat Dates Dates Bred Par. Date Ges. in Days Sex of Calf Sequel of parturition 1 4/17/24 1/12/25 270 2 4/22/25 1/5/26 e 256 Aborted Placenta passed :5 4/ 6 / 26 Abbl’eviations: Par. means parturition or calving. G08- means gestation or number of days carrying calf. BREEDING RECORD Cow NokBDG Breed. Owner. Name. Date. Reg. No. Has animal been tested for infectious abortion? Date Born. 12/23/22 Age to Date. Date Reactionfflfi ll’iVB See Charts PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Heat Dates. Dates Bred. 4/1 ‘7/2 4 Parturition No. 1 . Date. 1/12/25 Gestation in days. 270 Hours before passing?.-......51X..-ll€:.ur.s ........................... Aid? Fetal Membranes. ' Treatment? 1'; o t 1” e a tm Fin t O . 1? Condition? Disposal? Normal? normal Vaginal discharge? Diarrhea? Se‘luel to Parturition. Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Normal? No I'm a 1 Sequel to delivery. ' Dead? Die? Date and cause of death. Sex of calf ....... Post-partin-ient History of the Cow P aNiurition Date. Post-parturient Heat Dates Post-parturient Service Dates 1/12/25 4/2: /25 .4” ‘ . M’. 253 BREEDING RECORD Cow No.-.Li.-BBG ....... Breed. Owner. Name. Date. Reg. No. Has animal been tested for infectious abortion? Date Born. 12/25/22 Age to Date. Date ........................................ Reaction---EQSitiflfi .......... bird Ulléli‘t S PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Heat Dates. Dates Bred. 4/22/25 Parturition No ............. 2 .................. Date. 11/33/53 6 Gestation in days. 256 Hours before passing?-...----§.i.?< hour 8 Aid? Fetal Membranes. Treatment? 0 i 1 301° 1 0 AC 1 d Char-t made Condition? Recorded as a lesion reactor . Disposal? Normal? Adhesion to Hectum and Iiurnel Vaginal discharge? Va O-‘i r12 1 Di 8 0 ha 1" g; e Diarrhea? Secluel to Parturition. Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Normal? Very weak Segue] to delivery. Dead? Die? Date and cause of death. D’ l r ‘ SeXOfcalf ______ 1 er l/Q/db F1 +. ' . Post-parturient History of the Cow I 801 ”1 8 CO CC 1 t 1 S M -__ Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates 1/3/26 4/6/26 J: " {Il- ' -u‘u — :- Form 161—11 BREEDING RECORD _ WITH REFERENCE TO INFECTIOUS ABORTION \ Cow No.--M.--BBCr ...... AGGLUTINATION TEST Isolation of DATE Blood Milk Colostrum Ba ct. ab ort us Heat Dates Dates Bred Par. Date REMARKS lQfiL Jan. %**-- l ----- B.abortus orgtsism 1/3/26 8 mo. living 2 ----- never isolated fron calf. Died 3 ----- this animal or its 2 wks. later. 4 ----- issue, Ascities and Ebb. **p-- l ----- Coecitis. 2 ----- . 5 ..... 4 ----- liar . 'K' -- - - l 2 l 5 4 Apr. *P-" 1 4/6/26 2 3 4 day ‘K‘ p- - -' l l 2 3 4 June ‘*---- 1 l 2 5 4 July *p--- l 2 l is t 4 ; Aug . *p--- 1 ‘ 2 3 4 Septt *p--- l 2 3 4 Oct. **p-- 1 2 5 4 l": o v . 42- T-- - fl. 2 3 4 Dec. **T-- l 2 5 4 Abbmfihfimm: Par. means parturition or calving. Li 256‘1 CHART # BOF or amour _I_I_ D (First of consecutive fetal mem- branes. Parturition number one.) Calssification. Two plus resetor on June 1, 1926. Aborted a living Kale bapt. 15th, 1926. A period .5 107 oays during which she reacted. Animal has been a five plus reactor since aborting. l. apex of non-bravio horn. 2. Umbilical veins and arteries. 5 5. Apex of gravid horn. . 4. Yellow color indicates necrosis and shows ‘ the extent and location of the lesion. 5. White color indicates the interplacental thickening described by Williams and Hagen. 6. A normal cotyledon entirely surrounded by the interplacental thickening. The light placental infection probably accounts for the living calf. ‘I CHART # BOP OF GROUP E D (Second of consecutivr fetal mem- branes. Parturition number two.) 1. .2, 3. 4. 5. 6. 7. Apex of non-gravid horn Umbilical veins Umbilical arteries Apex of gravid horn Hyaline necrosis of infiltrating endothelial cells of the inter- placental part of chorion. Necrogis of nlacental areas. Semi-necrotic placental areas. “.1 x. BREEDING RECORD ZDU Cow No..-..B.Q.E ......... Breed Owner Name Date Reg. N 0 Has animal been tested for infectious abortion? Date Born 9/5/24 Date Reaction Po 8 i t 1V0 BREEDING HISTORY Par. No. Heat Dates Dates Bred Par. Date Ges. in Days Sex of Calf Sequel of parturition 1 1/7/26 9/15/26 252 Male Living and ac tive . 2 10/26/26 11/4/26 4/25/27 170 Female Dead @ delivery . Abbreviations: Par. means parturition or calving. Ges. means gestation or number of days carrying calf. BREEDING RECORD Breed. Name. Reg. No. Date Born. 9/5/24 Age to Date. Cow No.....I.3.QE...-....-.. Owner. Date. Has animal been tested for infectious abortion? Date ........................................ Reaction--..---Bn 31.12116.-." PRESENT BREEDING RECORD Heat and Breeding dates for the last pregnancy. Parturition N o. 1 Fetal Membranes. Chart made Recorded as a lesion reactor Sequel to Parturition. Heat Dates. Dates Bred. 1/7/25 Date- 9/15/26 Gestation in days. 252 Hours before passing?-.1i9..ri§l§lu£§§ S 383. .- Aid? Treatment? Condition? Ext en si ve n ec ro si s Disposal? Normal? Vaginal discharge? Som e d 1 so he. rge Diarrhea? Mastitis? Milk-fever? Pneumonia? Septicemia? History of the Calf Sequel to delivery. Sex of calf_- Ma 19 Normal? Normal but undersized Dead? Die? Date and cause of death. Killed 9 days later Post-parturient History of the Cow Parturition Date. Post-parturient Heat Dates Post-parturient Service Dates 9/15/26 11/4/26 *1 9.62 Form 161—H BREEDING RECORD WITH REFERENCE TO INFECTIOUS ABORTION C m ow No..- ............. DATE ‘ AGGLUTINATION TEST Isolation of H tDat 1) t B 1 P D H l 2 Blood Milk Colostrum Bact. abortus ea cs a cs re( ar. ate REMARKS mar . --.-nu fi—V Apr . ...-.0- May ----- June H--- July W l 1 Aug. “*p- 1 ‘ . " Aborted Sept. medic-w B.abortus isolated 9/14/245 living male {rem fetal mem- . Killed 9/24/26 rane. ‘ Par. No. 1 a , 2 2 . Oct . ***** “m 1. plates p03 1, 5 day 8.8 “in- 2 . " neg 0 1 ****P in " pan 4. l N 0V . “m . 8 ii Dee . «was 1927 J an. eta-4149* Feb . ***** MI 3]: t 'J.’ y Abbreviations: 1 . RF Par. means parturition or calving. 2 . RR 3- :TfF 9’. 6 3 Form 16l—H BREEDING RECORD WITH REFERENCE TO INFECTIOUS ABORTION Cow No ..... BOP--. ........ AGGLUTINATION TEST ,. . DATE Blood M'lk C 1 t 13153323333 Heat Dates Dates Bred Par. Date REMARKS l 0 OS rum 1 a Mar , *W* 1 2 5 4 Apr . *MHH“ 1 B .abortus i solat ed 4/23/27t Aborted 1'70 2 from fetus and fetal day female 5 Tombrane. fetus. Dead 4 @ delivery. May “*4”: l Parturition 2 No . 2 3 4 \\ Abbreviations: 31-. means parturition or calving. Jim—*fl Form 160-II BREEDING RECORD with reference to the AGGLUTINATION TEST OF THE DAM AND HER PROGENY 1926 FIRST GENERATION 1927 E:— \J Date Born FIRST GENERATION Ii??? h‘lar'eh April May June July August September October November December January I February 1 I '7I/23'7I/3~99 IDI) eeseeeee.. eeeeeeeeee eeeeeseees eeaeeeee}? eeeeezeees eeeeee~.r eraseee-ee eeeezeeeec asaeacece. e.e.¢.e 4c gee e—e.e eeeeaeeeee ! \g . _ SECOND GliNlCRA’l‘lON 1926 SECOND GENERATION 1927 .OHSDI‘iHQ of . Herd . - . . - . - . . .. . . (“\K‘No. PM. \‘o 3 Date Born Days 111 U tero Sire Sex Remarks No March April May June July August September October r\m ember December January February \\-;;;;Hm___ ‘ ' DD 3 l ‘ } \RLmio/Io/m : Male: Vealgd * DD 3 ; \KZFV 3/22 195 f: X. v-——. . A 1 DD 5 E l 1 r ; i I i l ' ‘ 1 i . l 5 i j l E ' I l ’ - s F ' . \J‘; 9/7/23 2'79 : 2 Male ~* Normal Sold ; 1 ; 3 ' l r! { DD Z ‘ l ‘ E ‘ ! I I i : : g 3 i 7' ......a fink—...- *'3H("X‘- I **%p— ‘Ié'ii'ifr'X'X- [‘1 6643*6343 6(‘95'24‘fi'X bees-2:4 '21— ‘léicidvk ir'hfr’n‘l'c \M/fl . 274. g i Female 1 Normal ; BOF I ; 7 « ’* ’7 “ . 1 . 1 DD l % Retained fetal ! g / ‘ membrane. i \iM/zs 277 Mal C Di ed 10/27 26 ; l i . l \sxm ........... Third .ien el‘ati in ,_ __.___,___ ,-_.__ . -7 ‘ I H (O \ L.) (“n '3 O3 252 Male 8 mo. calf k: H m o \‘ to q 170 Female Dead @ delivery. / / / / / I AbbreViation . ar‘ . . means parturition. ,___ ‘g._ 1.... 265 Microscoric study of interplacental lesions seen in the diseased fetal membranes. INTERPLAUENTAL LESIONS. Hyaline Necrosis of Infiltrating Endothelial Cells. PLATE 1' CASE # BAP l. Chorionic vessel 2. Zone of cellular debris showing chrOmatin material. 3. Surface of chorion, area of complete hyaline necrosis of infiltrating endothelial cells resulting in the thick leathery interplacental lesion indicated in white upon the various charts of diseased fetal membranes. 267’ INTB JiPLACENTAL LESIONS. Hyaline Necrosis of Infiltrating Endothelial Cells. PLATE LI ta." -“:£. L ..f‘lax .5.;L - rte; WV Detail of Plate I. 1. Chorionic vessel 2. Chromatin material of disintegrated endothelial cells. 5. Zone of hyaline necrosis of endothelial cell infiltration. l. 2. 3. 4. 6. 9. SUMMARY OF PLACENTAL STUDIES. Four and five parallel rows of cotyledons are the rule rather than six. The parallel rows usually occur in the gravid horn. Variations from parallel rows to scattered arrangement of cotyledons are to be found. Accessory cotyledons are not necessarily the result of disease processes. Naked areas upon the chorion are not necessarily the re- sult of prior injury to the endometrium. The significance of a naked area upon a fetal membrane can only be deter- mined by a study of consecutive membranes. All potential placental areas of the endometrium are not used at each consecutive parturition. Injury to the endometrium calls forth compensatory struct- ures such as accessory cotyledons and diffuse placental areas. However, placental areas unusually large in size have been seen as a compensatory arrangement for lack of placental areas. The interplacental lesion is not as significant as the placental lesion for the viability of the calf. R0 conclusion can be arrived at as to the significance of the location of the infection upon the membrane. Necrosis involving only the placental area and the adjacent carunculae is less significant than an endometritis because the carunculae are deciduous. The interplacental lesion seen in abortus infection is a hyaline necrosis of the chorion and of the infiltrating endothelial cells. or): 269 PART TIT DEUIDUA BASALIS BOVlS. IN TRODU CT ICN As memtioneg_in.the summary of my study of placental mem- branes (Part'll) I am herewith presenting clinical and photo- micrographic proof of the existance of deciduous carunculae in the cow and that the resulting lochia is physiologic rather than pathologic. élU PART 1T1" DECIDUA BASALIS BOVIS. Anatomical Comparison of the Human.and Bovine Placenta. Since I am adopting terminology used in describing the human pla- centa a comparative study of the human and bovine placenta is neces- sary e Prentissédefines the human placenta as follows: "The placenta is composed of the decidua basalis, constituting the maternal placenta and the chorion frondrosum and the placenta foetalis. The decidua basalis is equivalent to the maternal carunculae of the cow. The chorion frondrosum is equivalent to the fetal cotyle- donary areas of the bovine fetal membranes. The placenta foetalis is equivalent to the interplacental areas of the bovine fetal mem- branes. Decidua Basalis. This is the maternal placenta, it is differentiated into a com- pact layer and a spongy layer. The compact layer or basal plate forms the floor of the intervillous spaces. This is essentially similar to the fibrous connective tissue band seen at the base of the bovine carunculae. From the basal plate extend the spongy part of the decidua basalis. In the human this is different from the bovine. In the human pla- centa the spongy part consists of blood sinuses in which the villi of the chorion frondrosum are in direct contact with maternal blood. .Because the villi are free in the blood sinuses two provisions exist that are different from the bovine. These are the anchoring villi attached to the basal plate and the synctium of the villi that are free in the blood sinuses. The synctium serve the same purpose to the villi as the endothelium of the veins and arteries. In the spongy part of the bovine maternal placenta no attachments (Jf villi to the basal plate are to be seen. Blood sinuses are not :Ln.evidence. An elaborate intermingling network of fetal villi and Inaternal crypts are to be seen. There is not any direct contact be- twveen the maternal blood and the villi of the fetal cotyledon in the crypts of the maternal carunculae. The only evidence of anchoring villi in the bovine that I have found (see plate 'fil’) is what may be a temporary provision while taie new aborizations are developing along the chorionic projection. Comparative Classification of Placentae. DaviSSstates, "Huxley divided the placentalian mammals into two primary subdivisions, named deciduata and the nondeciduata. In the first or deciduata, the uterine mucosa undergoes rapid evolution and modifcations to form decidual tissue and the maternal and fetal parts become united. In the nondeciduate forms the uterine mucosa does not undergo this evolution and consequently the union of fetal and maternal structures is less intimate. With a few animals this classification is upset. This is true of the Sirenia, dugong, in which a nondeciduate zonary type of placenta exists. Birth does not release any of the maternal tissue, and in addition part of the fetal tissue is left in the uterus to be absorbed. The placenta of the mole is not shed at birth but becomes gradually absorbed by the mother. This type of placenta has been called contradeciduate. According to Dorlandethe definition of "deciduous" is as follows. "That which is eventually cast off." If we are going to have the classification according to time per- haps the quotation from Davis would hold true. However time should not constitue a basis for determining the deciduous nature of a structure. If the structure is eventually cast off it should be con- sidered deciduous whether it is immediately cast off as in the human or absorbed as he states in the mole or passes as lochia as in other animals. Placentation. LDevelopment of Bovine Placental Areas. 7 DeBruin, under nutrition of the fetus states during the pre- placental period nourishment is obtained from "Uterine milk, essen- tially composed of lencocytes in various phases, may furnish the :food. He recognizes that the fetus is free in the uterine cavity during the embryonic stage. 8 Beaver states that the ovum of the cow is embedded as in the human. My observations are that the embryo is free in the uterine cavity. 9 Miller states concerning villi upon the chorion "Th1 3 means that tflne villi which at first developed over the entire chorion disappear iirom.the greater part of it. Those villi which remain are limited tc> a definite area or areas and develop extensive aborization." l7 DeBruin states the chorion in the beginning of the fetal period possesses a number of dark-brown villi. Between these villi the cluorion is smooth and transparent. For this reason Franck7terms the snuwogh portion chorion loevis" and the villous are "chorion ville- sufl]. DeBruin considers that the foetal state starts around the twenty-eighth day. The statements of these authors without definite presentations of proof leaves it an open question. ' 271 So little is known concerning the development of placental areas that the presentation of some data may aid to give a lead to some one else who may happen to make a particular study of this. It may lead to an understanding as to why some placental areas are larger than others or eXplain the method of extensive aborizations seen in diffuse areas. It is quite plausible to assume that some areas in the bovine pla- centa are more attactive for the location of the chorionic villi be- cause of the variation in vascularity and type of mucosa etc. In my study of an intact fetal and maternal cotyledon of a three and one-half months fetus it is interesting to note what is going on at the periphery. The maternal mucosa has developed folds at the periphery of a placental area. These folds are entered by finger- like projections of the chorion. Aborizations are to be noted next to carunculae. :By this process of development any size cotyledon or diffuse area could be developed as the demand for nourishment would require. It is possible that the elongated uterine mucosa becomes the septa seen in the carunculae, and from this the maternal abori- zations develop. A study of the chorionic projection in the uterine crypts reveals the following. The epithelium of the chorionic projection consists of two types of cells. A smaller cell which is the more numerous of the two, and a larger cell appearing at regular intervals along the epithelium istpparently similar to Hofbouer's cells as des- cribed by Miller. The majority of these cells are apparently under- going.mitosis as two nuclei are seen in many of them. The adjacent maternal epithelium is undergoing a change apparently due to the presence of the chor§gpic projections. This change has 'been termed an erosion (Hallman of the maternal epithelium. A true erosion would producega necrotic zone as seen around the implanted Ihuman ovum. (Miller) The process going on in the maternal epithe- lium appears to be of a deciduous nature. The old epithelium is 'being replaced by a new epithelium due to the apparent new function of the area . The uterine glands show evidence of changed function. Part of the gland mucosa is replaced by large cells. These changes have not laeen observed in later stages or pregnancy. It is quite evident the «xhange belongs to the early stages of pregnancy when the fetus is fgeefi: the uterus. Possibly they play a part in the nourishment of , e e 1130 ‘27V23 EVOLUTION OF MA”EHNAL AED Fb'AL PLACENTA. PLATE _I Normal three and one-half months pregnancy. 1. 2. 3. A. B. C. D. Chorion Maternal placental area with fetal placental area intact. Chorionic projections entering the folds of maternal mucosa. See detail in plate 1:. See detail in plate E. See detail in plate :2. See detail in plate I. EVOLUTION OF MATERNAL AND FETAL PLACENTA. PLATE LI Detail of "A" in Plate 3;. Note the secondary branches of the projection next to the mater- nal struc ture . l. Chorionic projection. 2. Secondary branches. 3. Maternal aborizations. 274 EVOLTUION OF h‘lATERIIAL AND FETAL PLACENTA. PLATE III A Detail of "B" in Plate 3;. BShowing anchoring of fetal villi while the immature aborizations are developing. 975 EVOLUTION OF MATVRNAL AND FETAL PLACENTA. PLATE III B Detail of Plate Ill A 'l’Showing anchoring of fetal villi while the immature aborizations are develOping. 237'fi EVOLUTION OF MATERNAL AND FETAL PLACENTA. PLATEZIE Detail of "C" in Plate i. Showing cell structure of chorionic projection. 1. Large gnultinuclear cells similar to those described by Miller as "Hofbouer's cells." 2. Smaller and more numerous epithelial cells. 977 EVOLUTION OF MATERNAL AND FETAL PLACENTA. PLATE 2 A Detail of "D" in Plate 1. LDeciduous process of removal of old maternal epithelium. 1. Old.maternal epithelium. 2. New maternal epithelium. 3. Note absence of necrotic zone which would be present 19 a true erosion3 such as found in the Bryce Teacher ovum. 1. Dorland (6) 20 Miller (9) 278 EVOLUTION OF MATERNAL AND FETAL PLACENTA. PLATEEB Detail of "D" in Plate 1. 1. Old maternal epithelium a 2. Mitosis of old maternal epithelium 3. New maternal epithelium 4. Chorionic epithelium 9.79 EVOLUTION OF‘MATEHNAL AND FETAL PLACENTA. PLAT Iii I Showing normal structure of uterine glands in later stages of pregnancy and in non-pregnant state. EVOLUTION OF MATERNAL AND FETAL PLACENTA. PLATE VII Three and one-half months pregnancy showing type of cells to be seen in the gland mucosa in early pregnancy. These are the cells that probably supply the "uterine milk" of early pregnancy and account for some of the eroded material demonstrated in sections. 'The large cells are found in the gland nearest the surface of the uterine mucosa. 280 28] MATURE MATERNAL AND FETAL MEMBRANES INTACT. PLATE l F1111 term 0 l. chorion 2. Chorionic villi 3. Intermingling aborization of fetal and maternal placenta. 4. Connective tissue band 5. Zone of veins and arteries. MATURE MATERNAL AND FETAL PLACENTA INTACT. PLATE 1;: Detail of Plate I showing chorionic villi trunk. ' h. 1. Chorion 3. Aperizations of villi 2. V1111 4. Maternal aborizations forming the crypts. 9.82 283 NATURE MATERNAL AND FETAL PLACENTA INTACT. PLATE III Detail of Plate 1 showing lace like aboriaations in the deeper parts of the placenta. l. Chorionic villi as in plate I; 2. Aborizations of villi 5. Aborizations of maternal placenta. 9.84 PLACEI-ITITIS FOUND IN RETAINED PLACENTAS. PLACENTITIS PLATE :_I'_ Section illustrating chronic productive placentitis through the whole depth of carunculae. Full term. Normal parturition. Retained fetal membranes. 1. Chorion 2. Carunculae 3. Necrotic villi (darkest flecks throughout field are necrotic villi) 4. Chronic productive placentitis of maternal aborizations. (grey field surrounding necrotic villi) 285 86 mm: ENTITIS PLATE E . . » -. 1.:- ~153W.we , , .' J' 5 o / I . ‘ ' ' ‘ 5") v”?z‘ ‘ by ' ;. . . . I ' _ _. Q) ' .‘V. ~ } - l. .'o ’. " C e‘ ' Illustrating chronic productive placentitis of cases ADA,;DB and GO Detail of Plate 1. 1. Fetal villus 2. Necrotic fetal villi in a field of maternal tissue 5. Maternal aborizations showing chronic productive placentitis. Hellman.lo 287 mm ENTITIs PLATE ll'i Showing normal and diseased areas. 1. Normal maternal aborizations 2. Normal fetal villi 3. Necrotic fetal villi 4. Chronic productive placentitis of the maternal aborizations. PLACENTITIS PLATE ll Bacteria seen in section of Plate 3:. l. Villus 2. Maternal structure 5. Bacteria in villus 7 .v-r~ mi — 288 289 PLACENTITIS PLATE 2 Enlarged photograph of cotyledon from case # CBO shOWing hemorrhagic and congested zone in addition to evidence of Chronic productive placentitis. l. chorion 2. Hemorrhagic and congested zone. 3. Zone of chronic productive placentitis. PLAC ENTITIS PLATE E Photomicrograph of Plate E. 1. Chorion 2. Hemorrhagic and congested sane 3. Some normal architecture left 4. Area showing chronic indurative placentitis. spots are necrotic villi. The dark 9.90. PLACENTITIS PLATE VII . I: . ‘ .. o' ‘ . \ § «‘ ' I \ _ ;§$ };\flfifitVi.' . Detail of Plate E and E Showing normal fetal villi in a field of congested maternal placenta. 1. Normal villus (longitudual section) 2. Normal villus (cross section) 3. Dilated capillaries of the maternal placenta. PLACENTITIS PLATE VIII Detail of Plate I and LI 1. Hemorrhagic area showing blood cells. 2. Necrotic villi in hemorrhagic field. (\9 293 PLAUENTITIS PLATE :1} Q N 5",1 ' " r.T _ .11" 1’9“” 1 I. ‘ .I {21 "‘ A ‘ -w’ Detail of Plate 2 and E 1. Necrotic villi 2. Clumps of bacteria as seen throughout section. Higher magnification of this group of bacteria revealed a rod. B. coli and a streptococcus were isolated from this cotyledon. 294 PLACENTITIS PLATE 3 Detail of Plate _V_ and E 1. Necrotic villus trunk 2. Clumps of bacteria as seen throughout section. 295 RETAINED FETAL MEMBRANES. CASE REPORTS. 296 RETAINED FEIAL MEMBRANES. My observations have been that the seriousness of retained fetal membranes varies in different herds and within the herds, in dif- ferent individuals. The prognosis of retained fetal membranes is dependent upon the extent of the lesions found in the tract. If the infection is virulent enough to produce conditions in the uterus similar to the lesions I am illustrating in this part of the thesis, the prognosis must be considered unfavorable. Delayed involution due to pathological processes established dur- ing gestation and the presence of the pathogenic organism producing the lesions seen makes an unfavorable condition for full recovery. To the laymen and also many practitioners retention of fetal mem- branes is considered to be similar in all cases and that treatment should be the same for all. Comparisbn of results obtained by one practitioner by a certain method of treatment, which happened to be successful in a run of cases he had does not mean another practi- tioner can apply the treatment and get similar results. Several factors prohibit the application of any linenof treatment. 1. The operators judgement may not equal the other practitioner. 2. The Operator may be less skilled. . 5. The operators technic may be such as to make an unfavorable Egognosis in any treatment. e condition of the animal may be such as to make an unfavor- . able prognosis in any treatment. 5. Complications may have accompanied the parturition, lacera- tions, dystocia, and a host of other varying factors. 6. l“inally, the type of infection and extent of the infection producing the retention of the membranes. It may involve not only the carunculae but also the endometrium. 4. No doubt the ideal thing to do would be to remove the fetal mem- branes manually. In some herds and in individuals in some herds with a less serious type of retained placenta, I have found this to be un- wise and even impossible. Unwise because of the danger to the cow and impossible in some cases because of chronic indurative placenti- tis, and stony of the uterus. In stony of the uterus contraction is so slow as to make it impossible to reach and carefully remove the membranes without injury to the cow. Let us compare that which is removed and that which is left in many cases of manual removal. Even if it were possible to remove all of the membrane there is still left the bulk of fibrotic carunculae, perhaps equal in weight in many cases to the entire fetal membranes. 297 It is wisdom I believe, for the sake of the cOW, to let natural processes proceed, aided by non-inhibitory and non-irritating uter- ine antiseptics, until such time that the fetal membrane can be re- moved e I have found that in less severe cases, and by severity I mean the degree of placentitis etc, the membrane will loosen of its own accord and pass out. In the more severe types removal will be ne- cessary, when the membrane has loosened, because of the pendulous uterus found in atonic conditions. See photomicrographs illustrating lesions seen in placentitis re- sulting in retained fetal membranes. Cemparison with the normal architecture as seen in plate i (stature intact maternal and fetal membranes) will impress one as to why in- volution following retained placenta must necessarily be a slow process and even a dangerous period in the life of a Cow. It is unfortunate that methods of treatment in the field by the practitioner must of necessity be a one trip affair regardless of the type of retained fetal membranes. Any experimental line of treatment however that does not take into consideration the limitation of calls on‘a case will not meet with the practitioners approval. The schooling of selected clientle in the preliminary and after- care of cases of retained fetal membranes is practised by many successful veterinarians. RETAINED FETAL MEMBRANES. CASE REPORTS # ADA CAI CBO mew: 8 30i| RETAINED FETAL MEMBRANES. CASE # ADA The third parturition of this animal was normal. Notes taken concerning the appearance of the fetal membranes at that time were as follows. Normal parturition. Normal male calf, The afterbirth was passed within six hours. The gravid horn was normal except for an edematous condition ef the entire horn. The non-gravid horn had a naked area and some accessory and diffuse cotyledons. The parturition previous to this was a two hundred and forty- eight day dead delivery, followed by considerable discharge according to the caretaker. The case discussion presented here covers the fourth parturition. On November 20th, 1926, the two hundred and fifty-second gesta- tion day of the fourth parturition this animal aborted. A ease of dystocia with retained fetal membranes. The caretaker had success- fully delivered the twins (a male and a free-martin) living. On November 20th, 1926, at the forty-eighth hour, eXploration of the uterus revealed a firmly adherent fetal membrane. The cotyle- donary areas were pendulous from the walls of the uterus. There was apparently a lack of uterine tone. Manual removal was out of the question because of the extremely adherent membrane. All of the membrane could never have been reached at this time because the horns were not contracting very fast. To attempt manual removal would be to invite infection to the mucosa of the uterus. The infection no doubt was non-specific as the animal had been negative during the entire test. The only thing to do was to wait and see what nature would do. To see whether she would do as well as in case of number I BCI studied in this group. 011 and iedoform treatment as mentioned under case number I B01 was introduced into the uterus. 300 Because of the animals lack of uterine tone it was thought ad- visable to again enter the uterus to remove the placenta which by this time should be loosened if not already gone. I found the membrane free in the cavity. The maternal carunculae had sleughed as in M BCI but were much less disintegrated, apparently because of a more extensive placentitis. Only small button-like protuber- ances were to be found as in M BCI upon the uterine mucosa indicat- ing the position of the formerly attached carunculae. Oil and iodoform was again introduced and the case allowed to rest. See photograph of removed placenta. The prognosis for the case was unfavorable. She was examined again in January 1927 and apparently Was normal. The animal came in heat February 11th, 1927 and was bred. 302 RETAINED FETAL MEMBRANES. CASE REPORT # ADA PLATE 2; Plate 1: A. Umbilical veins and arteries. B. Maternal placental area attached to fetal placental areas. C. Interplacental area. Plate :1 A. Surface of one-half of intact maternal and fetal placental area. B. Cross section of A showing firm consistency. Note: Removed on the ninth day. The membrane was free in the cavity with the carunculae firmly attached. DB ASE REPORT OF # C 3‘ 305 RETAINED FETAL MEMBRANES. CASE REPORT # DB PLATE g Plate 1 .A museum jar of fetal membrane with carunculae attached. Plate I; Cross section of cotyledons from those seen in plate:i. A. Chorion B. Carunculae. Breed Name Reg. No lDateBorn May 11, 1919 BREEDING RECORD Owner Date 7 ___“.— -.. T 366 Cow No.-.DB ........... Has animal been tested for infectious abortion? Date BREEDING HISTORY React-ion Pesitive Par. N 0. Heat Dates Dates Bred Par. Date Ges. in Days Sex of Calf Sequel of parturition l ll/ll/2o 11/29/20 1/9/21 5/5/21 9/14/21 132 .X Aborted 2 10/13/217/11/22 292 Female Dead at delivery 3 1/2/23 9/21/23. 263 Twins F Dead at delivery Retained placenta. Placenta areas sloughed. 4 4/5/23 12/22/2j 9/9/24 262 Female Dead at delivery 5 ll/5/24 12/2/24 9/3/25 2'76 Female Normal (sec) 6 10/28/2 ll 24 2 ‘ 1 ll 26 3/29/26 4/22/26 1/15/2'7 269 Female Normal. Retained placenta. 7 4/16/27 Abbreviations: Par. means parturition or calving. Ges. means gestation or number of days carrying calf. ... -__—RI RETAINED FETAL MEMBRANES. CASE # DB. Strong Bang titre. See attached breeding history. First abortion was probably due to Bacterium abortus. The case discussed here is the sixth parturition. A two hundred and sixty-nine day normal delivery. Membranes retained. They were firmly attached as in case of number ADA. Treatment same. Prognosis name. Last time examined on April 15th the genital tract was apparent- ly normal. Gave hormone to stimulate heat period as the animal has not had an apparent heat period. Bred April 16th, 1927. See photograph of recovered membranes. No material could be obtained for bacteriological examination to determine causative agent of retained membranes. CASE #M CBO Report of case from which the hemorrhagic cotyledon was obtained. History Negative to B.abortus. First calf heifer. Twenty-seven months of age. An eXperimental animal extremely emaciated at time of parturition. Two hundred and twenty-eight day non-specific abortion. Case of dystocia, (an edematous fetus, dead at delivery) retained placenta, eversion of uterus. This animal lived twenty-eight days and died of necrotic pneu- monia. Normal involution had taken place. Uterine mucosa was apparently normal. The placental areas were 1/16 to 1/4 inch in diameter. Bacteriological findings. Streptococcus and B.coli isolated from an intact cotyledon taken at time uterus was everted. 308 RETAINED FETAL MEMBRANES. CASE #M CAI Case with uterine.infection similar to #l 080. History Negative to B.abortus. First calf heifer. , Experimental animal similar to #M CBO. Parturition. Two hundred and fifty-eight day delivery of dead female fetus. Retained placenta. At the twenty-fourth hour of retained fetal membranes the uterus was entered and an attempt made to determine the possibilities of removing the afterbirth. The membrane was firmly adherant. Since the fetal placenta was too firm to be removed manually at this time a cotyledon was taken for bacteriological and pathologi- cal examination. This cotyledon was removed from the cervical end of the gravid horn. It was removed without destruction or tearing or Otherwise injuring the maternal tisSue. This may be accomplish- ed by removing the cotyledon above the connective tissue band. In some animals this may be done with the thumb. In others removal is impossible without injury to the mucosa. Fetal Placenta. The fetal placenta was adherant to the maternal placenta. The interplacental portion was thin and easily torh. It was of the type in which about all one can take away is an occasional handful con- sisting of the placental arteries and veins. ‘Treatment. No attempt was made to remove the membrane. One and one-half pints of oil and boric acid was injected. Post Mortem. .Animal slaughtered for beef forty-eight days after parturition. Normml.involution of the horns had taken place. 309 CASE #M CAI (cont.) Macroscopic Examination of the Genital Tract. Vulva Vagina Cervix L .Iiorn LeOVidugi L.Ovary R.Horn BoOViduc-‘E R.Ovary Normal At the time of parturition the vulva was severely torn. Perfect healing had taken place. Records of lacerations were not noted previous to post mortem examination. No lesions or evidence of lesions were to be seen at the time of post mortem. This was the non-gravid horn. No afterbirth was removed manually from the horn. Normal involution had taken place. The placental areas averaged 3mm in diameter. The uterine mucosa of the horn was normal in appearance. The surface of the horn was slightly moist. No excessive fluid or pus was seen. Normal 0 2113-0111 by 1'51é'cmo Normal Normal involution for the period. The horn was 2% inches in circumference at the bifurcation of the horns. The uterine mucosa was the same as in the non-gravid horn. Normal to macroscopic examination. Size was 4cm by 2%cm. CeLePe 20m by 201110 Fluctuating cyst-like structure at the end opposite to thc COLOPO 13130171 by lficmo The ovary in contrast to the left ovary is very flacid. Bacteriological examination of cotyledon. Streptococcus B.coli isolateda. Microscopic examination. Lesions similar to #M CBO RETAINED FETAL MENBRANES. CASE # GO Born September 1, 1919. Strong Bang tire. History. Parturition #1 165 day fetus. Parturition #2 258 day male---Killed. Parturition #5 271 day female-Normal. Parturition #4 281 day female-Normal. Parturition #5 284 day male-~-Normal. Retained fetal membrane at the fifth parturition. Plateji of microscopic study of placentitis was taken from this case. A severe case of chronic productive placentitis. Non- specific infection as abortus was not isolated from the cotyledon or placenta. Involution was very slow. The endometrium no doubt was also involved. 311 Clinical Proof of Deciduous Nature of Bovine carunculae. Case # M BCI Case # BAC 212 LOCHIA. 313 LOCHIA. In literature pertaining to lochial flow of the human female dur- ing puerperium there is perfect harmony of Opinion. In veterinary literature the subject is in a state of discord. This state of affairs is due to the lack of knowledge or proof of the true physiological processes accurring during the puerperium. The less intimate contact with the discharges from the bovine female during puerperium is the reason why such a difference of opinion exists. If the handling of the bovine female was as careful and as intimate as in the human, during puerperium then perhaps less differ- ence of opinion would exist. 7 Concerning lochia DeBruin states, "The lochia are a chocolate colored or only faintly colored fluid consisting mainly d? uterine milk and also uterine epithelium, leucocytes and fat. Williamszstates,"After the eXpulsion of the fetus and its membranes, there inevitably occurs in all animals a more or less recognizable discharge from the uterus, consisting of blood and fragments of the uterine mucosa or of villi of the placenta. The discharge is known as the lochia." "In the mare lochial discharge should not be re- cognizable except after a few hoursfl nor should it be conspicuous in the cow for more than a day or two. He goes on to say, "So far as can be determined at this time the condition above described is the basis for the dirty, scarlet-gray discharge so often seen during the puerperium and generally desig- nated by veterinary obstetrists as lochia. It is commonly regarded as physiologic but in reality is pathologic. Commonly in the milder cases no sloughed cotyledons are recognized and perhaps the gross necrosis fails, but there are hemorrhages into the crypts with per- haps important cellular necrosis and decomposition of the blood coagula which, mixed with exuded fluids, constitute the scarlet dis- charge, or lochia or the puerperal state." 1 Fleming states, "That lochi may continue foridays or weeks and that seven or eight quarts of lochial discharge from the uterine cavity have been removed from the mare three days after parturition. In the cow following a normal parturition I do not look for the greatest lochial flow during the first two days of puerperiun. The true lochial flow varies with the individual and is dependent upon the rate at which the carunculae disintegrate. .314 The color scale varies from red, chocolate, grayish red, gray and white to clear mucus. The color is dependent upon the time follow- ing parturition that one observes the discharge. The lochia consists of disintegrating carunculae. At times I have observed pieces of the disintegrating carunculae which still showed the outline of the crypts. If the carunculae are normal and do not show an excess of denser tissue, such as is found in chronic product- ive placentitis, then the lochial mass will be more homogeneous in character. 315 DEUIDUA BASKLIS BOVIS. CASE # M BCI DECIDUA BASKLIS BOVIS. CASE # M BCI On April 10th, 1926 animal number H BCI aborted a forty-five pound, two hundred and twenty-nine day fetus. Animal was neg- ative to abortus until April. In April and since then the Bang titre has been strongly positive. The fetal membranes were retained. At the forty-eighth hour manual removal of the:membranes was attempted but they were too firmly attached to attempt removal. A half ounce of iodoform in gelatine capsules was placed in each horn. This was followed by a quart of merusol (mineral oil) and the case allowed to rest. The capsules and the oil Were put between the chorion and the endometriun. Because of the firm attachment of the membranes in this parti- cular case I deemed it ncesssary to explore the tract again. On the seventh day after parturition I explored the uterus and found that the membranes were free in the cavity. I removed that which had not disintegrated and then drained the disintegrated mass as involution had progressed sufficiently to allow me to reach the apical end of the horn. It is to be noted that at no time was there any odor from the case. The disintegrated material found upon the seventh day had the grayish appearance of lochial fluid. I removed three quarts of this fluid. Noticing that the non-disintegrated portion of the fetal mem- branes had portions of the carunculae attached to them I again entered the uterus and explored the entire tract. Very little of the carunculae were left and in the majority of cases only a firm area indicating their point of attachment remained. I did not attempt to douche the animal at this time but again put in the same amount of oil and iodoform. Fourteen days later I douched the uterus with Lugols solution. Uneventful recovery resulted.and the animal was bred July 7th, 1926. She was pro- nounced pregnant to this date on October 1926. we :20 mg I60 ... . +r_ At.» | DECIDUA BASALIS bOVIS. PLATE i: Case # M BCI Photograph of case # M BCI at the time of abortion followed by retained placenta. This animal was being used on a feeding experiment. 319 BCIDUA'BASRLIS BOVIS. PLATE E Case # M BCI Showing museum jar full of partially disintegrated carunculae, with some fetal membrane attached. These carunculae together with approximately three quarts of disintegrated material were re- moved unon the seventh day following the abor- tion with retained fetal membranes of cow shown in Plate ;, 320. w- 321 £36! DECIDUA BASALIS BOVIS. PLATE III Case # M BCI Photograph of case # M BCI after normal parturition. First and only service July 7th, 1926. Freshened April 1927. Ninty— two pound calf. (Bee copy of fetal membranes on the next page.) 322_ fl. v. . \ Q. I ..u ..‘O- 3:2 4 DECIDUA BASALIS BCVIS. PLATE g Case # M BCI Chart of fetal membrane of the normal 1927 parturition following conception to the first service after the April 1926 abortion with re- tained fetal membrane, and known disintegration and passage of the carunculae as shown in Plate “ll of this case. 1. Apex of non-gravid horn 2. Umbilical vein 3. Umbilical arteries showing anastomosis 4. Apex of gravid horn 5. Necrotic foci in placental areas. DBCIDUA BASALIS BOVIS. PLATE 1 Case #M BCI Showing the umbilical arterial anastomosis shown on chart of #M BCI. (Formalin fixed specimen.) I have found the anastomosis to be quite common. It is obscured in many cases by the membranes. 1. Umbilical veins from non-gravid horn. 2. Umbilical artery to non-gravid horn. 3. Anastomosis of arteries. 4. Umbilical vein from gravid horn. 5. Umbilical artery to gravid horn. 6. Interplacental membrane or part of chorion. 7. Wire through anastomosis to show connection. 326 ii 327 DECIDUA BASALIS BOVIS. CASE # BAC Macrosconic and microscopic proof of the deciduous nature of the bovine carunculae. 998 DEC IDUA BASALI S BOVI S . CASE # BAC See chart of placenta under part D of Group E. On January 10th, 1927 the animal aborted a two hundred and forty- five day fetus which was dead at delivery. Since the animal had four secluded teats and no milk, she was slaughtered. The animal was killed ten days after parturition. At her death at least a pint of lochia passed from the vagina. The lochia was of a reddish chocolate color in which were suspended lighter colored particles. EXTENT OF INVOLUTION IN TEN DAYS. The gravid horn was 74 cm in length. Its greatest circumference was 21.50m. This circumference was taken 44cm from the apex of the gravid horn. The non-gravid horn was 500m in length and 14cm in circumference 290m from the apical end. Upon Opening the horns I saw about 250 cc of the same lochia seen on the killing floor. The carunculae were reduced in size. The larger carunculae were 4cm by 5cm across and Item high. The aver- age size was Son by fem high. In the apex of the gravid horn the carunculae still showed evi- dence of necrosis; the dry firm material similar in appearance to the necrotic areas of the fetal placental lesions. Allctarunculae were undergoing disintegration. Since the fetal membrane chart of this case gave me evidence as to which areas were normal at the time of parturition I chose ma- terial from areas free from disease. Carunculae were taken from the cervial end of the gravid horn and also the non-gravid horn. These were fixed in Zenkers fluid and finally sectioned. See phetomicrographs of this case. See photographs of the disintegrating carunculae of this case. Dr. Hallman had reported the process of disinte ratin caruncula in the July 1924 ileue‘of the Cornell Veterinarias. Hofiever he 6' left it as an open question and in the October 1924 issue of the Cornell Veterinarian Williams made statements absolutely contrad- ictory to the observations presented by Dr. Hallman. 399 .I 330 DECIDUA BASALIS BOVIS. PLATE 1 Because the carunculae were easily disintegrated by handling, the photograph was taken with the specimen in the museum jar. l. carunculae 2. Uterine mucosa DECIDUA BASALIS BOVIS PLATE _11' Case # BAG ‘ _ —-——-_- ___‘_-_.__' ,4 ’. ,7 V. —x _ - . . ~"‘"‘ -,, - ‘1 c! ék‘ 5:, \" -" u‘.“ r‘hfiv‘: V '4 ' ‘s Section of disintegrating carunculae. l. Disintegrating zone. 2. Connective tissue band. (Basal plate.) 3. Uterine mucosa. 4. Uterine glands. 331 232 DECIDUA BASALIS BOVIS. PLATE III CASE it; BAC \ .- .—.c _‘_M*_. Detail 5: Plate ““111. 1. Showing vestige of former structure. 2. Cellular infiltration. 333 DBCIDUA BASALIS BOVIS. PLATE fl Case # BAG Detail of Plate E. 1. Cellular infiltration 2. Hyaline degeneration 5. Giant cells. 334 wfivvv _ DECIDUA BASALIS BOVIS. PLATE V Case # BAC A ...l 0’. Pr IV Detail of Plate 1. Giant cells of the foreign body type were numerous throughout theistructure. DECIDUA BASALIS bOVIS. PART III A study of the evolution and involution of the bovine placenta. CONCLUSION The carunculae of the involuting bovine uterus are eventually cast offl and passed from the uterus as lochia. The lochia seen following normal parturition is physiologic and is the result of physiological cellular pro- cesses of reduction. 7. 8. IO. 14. Udall, R.R. Williams, w 01—. $3236 BIBLIOGRAPHY. Editorial. Cornell Veterinarian. January 1927. Studies in Genital Diseases. Cornell Veterinarian. Vol.XlV No. :2 p. 524, 526 and 548. October 1924. Huddleson, I.Forest, Hasley D.E. and Torrey, J.P. Prentiss, C.W. Davis, James E. DO rland, W 0A or: o DcBruin, M.G. Beaver, Donald C. Bailey and Miller Hellman, E.T. Craig, J of... Sisson, S. B61101", Ko-Fo Further Studies on the Isolation and Cultivation of Bacterium Abortus (Bang). Journal of Infectious Diseases. Vol. 40 No. 2 p.352-sea Feb. 1927. Text-Book of Embryology. p. 245 1915 Anatomical and Clinical Studies upon 875 Placentae. The American Journal of Obstetrics and Gynecology. Vol. Vlll No.6 p.4 July 1924 American Illustrated Medical Dictionary. Thirteenth Edition. Bovine Obstetrics. p.16-25 1901 Bacteriology and Pathology of Sterility in Cattle. TCCImical BUlletino 1100 5 p. 2]. Text-Book of Embryology. Fourth Edition. p.578, 584 and 5850 Further Studies in the Disease of the Reproductive Organs of Cattle. Cornell Veterinarian.(Reprint) p.255 July 1924 Veterinary Obstetrics. (Fleming) Veterinary Anatomy. p. 518 1911 Anatomical, Physiological and Bacteriological Researches of Pregnancy and Birth in Domestic Animals. Cornell Veterinarian. Vel.XVlI1 No.1 p.30 January 1927. The value of Placental Examination for the Diagnosis of Infectious Abortion of Cattle. Cornell Veterinarian. Vol.XV1 No.4 p.245 00 tObfiil" 1926 o ACl‘ZIé‘O‘JJLL‘DCEIsIEN T . Grateful acknowledgement is given to Dr.E.T.fiallman and Dr.I.Forest Huddleson for assistance and suggestions received and to Miss Eugenia McDaniel of the Department of Entomology for making photomicrographs illustrating this thesis. 3 3 TEC HN 10 "A record of the instruments and methods developed while attending Michigan State College. [-1 Ol’nl‘tll; a... VAGINAL SPECULUM AND CERVICAL FORCEPS (Original) Instruments made to aid me in the treatment of cases. 1. Open speculum showing light carrying rod with battery directly attached. 2. Closed speculum with battery cord attached to light carrier. 3. Tenaculum pointed forceps. 4. Knowles jaw forceps with removable handle to allow passage of speculum over forceps. R41 MODIFIED ASPHALTUM FOR SEALING MUSEUM JARS. (Original) Considerable difficulty had been experiencied by the path- ology department to obtain a perfect seal for the museum jars. The perfect material must be easily applied, should be in- expensive, must have excellent sealing properties, including adhesive properties and must not harden with age. I knew that asphaltum would not harden with age. A supply of asphaltum had been bought by the department years age and discarded for sealing purposes because they found it difficult to apply to the Jars. I found out that Xylol was an excellent solvent for asphaltum. Using a minimum amount of Xylel and a slew heat I obtained a pre- duct that could be easily handled and applied. When applied I found that its adhesive properties were excellent. The method has been in successful use for three and one-half years in the museum of the pathology department. TISSUE WASHING RACK. (Original) The original rack was made in 1922 out of a one-half inch brass pipe, fifteen inches long. The pipe is closed at one end and fitted to attach rubber tubing at the other end. Twelve one-eighth inch tubing pieces one inch in length are placed in a row to allow two- hole rubber stoppers to be placed in a row. These stoppers -are of a size that will fit a one inch shell vial. PARAFFIN MOLDS. (Original) To replace the paper paraffin molds and forms used to make them I made the metal paraffin molds illustrated. They are made from one inch square tubing with one side cut away. The ends are movable to adjust for the size of the tissue to be embedded. The idea originated with me while working for the Battle Creek Sanitarium. The first ones were made out of tin. The devise has been in successful use for over three years. NEW ANIMAL BOARD. (In co-operation with Dr. Huddleson) My contributions to this board were the swivel ratchet arms front and rear. Also the slide lock- ing loop to fasten the limbs of the animal. EOSIN The present eosin stain used by the pathology department was decided upon by me. This was brought about by the fact that we ran out of our original supply of German eosin dyes. No one realizes the hard time I had to run down a stain that answered to the staining properties of the original Grubler stain e I made two trips to the Battle Creek Sanitarium. Two stains were obtained. Neither of these answered the purpose. ' While in New York I spent considerable time locating dyes. I brought back an Eosin Bluish Alcohol Soluble and also an Eosin Bluish Water and Alcohol Soluble. These were unsatis- factory. Six stains were then obtained from the Will Corporation. These were tried on various tissues but were less satisfactory then the Battle Creek stains or the Grubler stains obtained in New York. Three old stains found in the bacteriology store room were then tried with the same discouraging result, Finally we obtained a Will Corporation Eosin Yellowish and a Harleco eosin yellowish stain. The Will Corporation stain was fair but the Harleco stain not only looked like the original German dye but had all the excellent staining properties. METHYLENE BLUE (Original) Reduced Alkalinity. The proportion of Potassium carbonate to methylene blue in ‘Unna s stain was reduced from one gram of potassium carbonate to five tenths of a gram per one hundred cc stock solution. Old Formula New Formula .1 gram methylene blue 1 gram methylene blue .1 gram potassium carbonate .5 gram.potassium carbonate 100 cc distilled water 100 cc distilled water This proportion was eXperimentally determined to give the best staining results. 3435‘ MINERAL OIL INFILTRATION. Prior to Oven Paraffin Infiltration. (Original) Knowing that tissues easily harden when infiltrated in the paraffin oven, I decided to infiltrate my specimens with an unheated mineral 011. Two immersions are made to get out all the cedarwood oil before putting the tissues in the even for final infiltration. Shorter time and fewer changes are necessary in the oven. I have successfully stored tissues several months in oil. As tissues vary in procedure time according to size and kind, the real purpose of mineral oil infiltrations is to en- able one to have a rest period before final infiltration when a group of tissues are being put through the alcohols and clearing oils. FILING OF PARAFFIN BLOCKS OF EMBEDDED TISSUES. (Battle Creek Method) Originally the paraffin blocks of the pathology department were stored in shell vials. When several specimens were placed in one vial and the paraffin melted, it was a difficult matter to identify a particular piece of tissue. After melting and re-embedding several dozen blocks I decided to store them in coin enve10pes, according to the method used in the laboratories of the Battle Creek Sanitarium. The blocks do not melt in the coin envelOpes. More tissues can be stored in a given space. An envelope is cheaper than a shell vial. 347 USE OF SODIUM THICSULPEITE. (Mallory-Wright) When I started staining slides in the pathology department, removal of the iodine from the slides during the staining pro- cess was by the aqueous method only. After being troubled by loss of sections and loss of time in the procedure, I adopted the method of using sodium thiosulphite to remove the iodine from the sections. I found that the method was faster than the older method and the staining properties of the tissues were not impaired. USE OF KEUTBAL XYLOL. (Original) To overcome the possibilities of introducing acid over into the neutral balsam, I tested the Xylol used, to determine whether it was neutral or acid. I found that the reaction was acid. .The Xylol was made neut'al by use of Anhydrous Sodium Carbonate. This step has materially aided in preventing loss of the nuclear stain during the staining process, and possible loss when the section is mounted in balsam. 4 IVEETIIUD OF NUInibEhIEJG SLIDES FOR STUDEKT BOAES. (Original) The slides are numbered according to numbers in the box making it easy for the student to replace the slide. The numbers are easily seen because they are made of colored paper. The paper numbers are picked up with a pin and placed upon the slide that carries a drop of weter at the place where the numbers are to be fixed. The numbers are then dried with blotting paper. When dry, a film of celloidin is painted over the figures. 3454 I . l . « ‘ a ' - ">- ,. .'. . . - ~ I A A _ v . .. _ . —.' " " . .' ,1, . ' n A 'i a _ v .. '- . 4» . ‘..I r'." .T' g," . ‘. ' w‘vfi .A' . . \ 3... ".- , ‘ ’ ' _ ‘ ' '. ‘J .-"‘ 2" ' . ‘“31?&‘:§~:t~, It); i . 9.3.11. _ tw‘?‘-‘."s ;‘ gig . . , . g-A‘ 3%". I : 9. .‘figifiw‘m‘: F ‘1 ‘.'.' " -..- ‘ ’ '. .'.’l.'-" I“. C "3". k. 3' .‘> .' I . _ ‘h .‘1‘ fi‘ll “A 49" “g“; 233...“; j Y. .. C W . :).*’\::J‘ ‘. 3"." ' A" use“ ~ ‘ 1T .- —‘—3~ - I ll '_ _‘ ’_':V‘o.. ’- '1 43 4’ I ' V. . a t 0*“? . ,.; .4~‘ .‘-‘+ ‘- ' ~ 1‘ " ' ':- ' ‘ ‘ ' ‘ .I' . .’ . ' ~ , ‘ . avfyt ' ' ‘..h . . “ " 2'-J - . ;- ~'" ' " ‘ . . ' - - ', , , _ v ' .‘ .- . . " *i’ . ‘*~ - -- A- - -~ ;az,w..e.»..,-a- .1 z. r . . > . .3” .‘ . ._- ._ .' ' . ~v ”.79932'3‘S'f '1‘“; . -; 1' .‘f .‘i'fi. ~‘ é -1), ‘ ’ VI ' v ,rflzl- " ‘1 .' .v T t w . . ' 1 g.. . - l.. r . "I v ROOM USE 0PM INI'ER-UBRARY LOAN 2 5 sat-’40 - "WW-'7‘" “"Tfiliiiiiuflflllufitfl{fliujflfifllflflyflflfimi‘s