3 53'? Th‘??. $382.55 AT ‘3‘?! RAT108 T {)N AMELK FLQW RATE$ 3...: ? Egg-‘5': '31“!!! . :iv. 3 L.‘ th'y- b.3533“ F ‘V'. _ " .vv—w LIBRARY Michigan State University ABSTRACT THE EFFECTS OF THREE PULSATION RATIOS ON MILK FLOW RATES by Eldon LeRoy Spicer To compare the effects of three different pulsation ratios on the average and peak milk flow rates is the object of this study. Thirty-four cows in different stages of lac- tation and representing three of the dairy breeds, 2h Hol- stein, 6 Brown Swiss, and S Jerseys, were used in this experiment. Three pulsation ratios (the milking to rest ratio) were used in this study with two of these, a 50:50 ratio and a éu:36 ratio, applied to the entire udder while the third was a variable ratio using a 6h:36R (R indicates rear) ratio for the rear half, and a 36:6hF (F indicates front) ratio for the front half. These pulsation ratios were obtained by the use of a timer-converter and by modifying the standard De Laval milker claw and pulsator. The milk flow data that were recorded in graphic form on a tape by the recording Milk-O-Meter were studied and con- verted into milk flow rates. Samples of milk were taken from each cow at each milking to determine the effect of each pulsation ratio on udder irritation which was determined by the use of the Milk Quality Test. The irritation study was a separate project being carried on simultaneously by another Eldon LeRoy Spicer investigator. Slightly higher average and peak flow rates were obtained when the cows were milked with the 6h:36 pulsation ratio than when milked with the 50:50 or 6h:36R - 36:6uF ratios. The 6h:36R - 36:6AF pulsation ratio gave the lowest average flow rate. With the Brown Swiss and the Jersey breeds the éu:36R - 36:6MF gave a slightly higher peak flow rate than that obtained with the 50:50 pulsation ratio. Analysis of variance showed a highly significant dif- ference between the breeds in both average and peak flow rates, but no significant difference between the ratios. The results of the Milk Quality Test indicated that the 6h:36 pulsation ratio caused the least irritation, while the 50:50 ratio caused the greatest irritation. THE EFFECTS OF THREE PULSATION RATIOS ON MILK FLOW RATES By ELDON LEROY SPICER A THESIS Submitted to Michigan State University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Department of Dairy 1962 ACKNOWLEDGEMENTS The author wishes to express his sincere appreciation to Professor W. W. Snyder for his very helpful direction and assistance throughout this study, to Dr. E. Weaver and Alma M. Campbell for reading the manuscript, to Dr. W. F. Buchele for his technical advice in setting up the pulsation ratios, to R. Thomas for his assistance in handling the statistical data for the study, and to M. A. Wilson for his assistance in collecting the data. The author is indebted to Dr. C. A. Lassiter, chairman of the Department of Dairy, for making facilities and animals available, and to D. V. Armstrong, herdsman, and his assist- ants in the barn who aided in the collection of the data. Also the author is indebted to C. M. Crosby and the De Laval Company for supplying some of the equipment needed for this study. The author appreciated the financial support provided by Emmanuel Missionary College which made his study at Michigan State University possible. Finally, the author is deeply grateful to his wife, Anna, for her encouragement and assistance in the prepara- tion of this manuscript. ii TABLE OF CONTENTS Page INTRODUCTION . . . . . . . . . . . . . . . . . . . . . 1 REVIEW OF LITERATURE . . . . . . . . . . . . . . . . . History of the Milking Machine. . . . . . . . . Functions of the Milking Machine. . . . . . . . The Vacuum Pump . . . . The Teat Cup Assembly . The Pulsator. . . . . The Vacuum Regulator. Weighing Devices. . . . . . . . . . . . . . . . Factors Affecting Milk Flow Rates . . . . . . . Day-to-Day Variation. . . . . . . A. M. and P.M. Milking . . . . . . Stage of Lactation. . . . . . Variation Among Individual Cows . Variation Between Breeds. . . . . Inheritance . . . . . . . . . Variation Between Quarters. . Milking Rate and Yield. . . . The Sphincter Muscle. . . . . . Vacuum Levels and Pulsation Rates . . S o o o o Pulsation Ratios and Vacuum Levels. Effects of Stimulation Before Milkin 5&3 EXPERIMENTAL PROCEDURE . . . . . . . . . . . . . . . . 21 Pulsation Ratios. . . . . . . . . . . . . . . . 22 The Recording Milk-O-Meter. . . . . . . . . . . 31 Procedure for Collecting Data . . . . . . . . . 31 Milking Rates . . . . . . . . . . . . . . . . . 33 The Milk Quality Test . . . . . . . . . . . . . 35 RESULTS AND DISCUSSION . . . . . . . . . . . . . . . . 38 Average Flow Rate . . . . . . . . . . . . . . . RO iii TABLE OF Peak Flow Rate. Machine Time. Udder Irritation. 8111qu HY. O O O O 0 CONCLUSIONS. . . . LITERATURE CITED . CONTENTS--Continued iv Page R1 15 LL? 1+9 50 51 TABLE II III IV VI LIST OF TABLES Page The Order in.Which Data Were Collected for Three Pulsation Ratios. . . . . . . . . . . . 32 Average Flow Rates and Peak Flow Rates Ob- tained From Three Pulsation Ratios. . . . . . 38 The Mean Differences in Average Flow Rate and Peak Flow Rate For Three Dairy Breeds . . Al The Average Milk Yield Per Milking and Machine Time As Obtained From Three Pulsa- tion Ratios O O O 0 O O O O O O I O O O O O O [4.3 The Average Milk Yield and Machine Time By Breed As Obtained From Three Pulsation Ratios. O O 0 O O O O O O O O O O O O O O O 0 LL; The High, Low, and Average Machine Time for the Holstein, Brown Swiss and Jersey Breeds. O O O O O O O O 0 O O 0 O O O 0 0 O O [4.6 LIST OF FIGURES FIGURE 1 The modified De Laval timer-converter used for obtaining pulsation ratios. . . . . . . 2 The converted standard De Laval milker claw used to obtain the euz3e pulsation ratio. . 3 The converted standard De Laval pulsator used to obtain the 6h:36 pulsation ratio. . h A device used to by-pass the air ports of the standard De Laval milker claw . . . . . 5 A device attached to the standard De Laval milker claw, used to by—pass the air ports. 6 A graphic recording tape used with the re- cording Iqilk-O-Metero o o o o o o o o o o o 7 A device used to facilitate the reading of the graphic tapes . . . . . . . . . . . . . vi INTRODUCTION Now that the dairyman has firmly established the milking machine on the modern dairy farm much study is being done to make this piece of equipment even more efficient for removing milk from the udder at a faster rate and with the least possible irritation. Various methods have been employed to speed up the rate of milk flow from the udder. Several investigators have increased the vacuum level to overcome normal resist- ance to the teat sphincter, others have combined higher vacuum levels and faster pulsation rates, and some have given study to the effect of pulsation ratios on milk flow rates. The object of this investigation was to ascertain the effect of three different pulsation ratios upon the rate of milk removal from the udder. REV IEW OF L ITERATURE The literature will be reviewed with respect to the effects of various factors on milk flow rates during milking. These factors include day to day variation, a.m. and p.m. milking, stage of lactation, variation among individual cows, variation between breeds, inheritance, variation between quarters, milking rates and yields, the sphincter muscle, vacuum levels and pulsation rates, pulsation ratios and vacuum levels, and stimulation before milking. History of the Milkigg Machine Erf (17) described many types of pressure devices and milking tubes designed to remove milk from the udder. He stated that American inventors attempted to perfect milking machines as early as 1819. No good descriptions or illus- trations were made of these machines. One of the simplest schemes for milking, aside from hand milking, was the use of straws inserted into the cow's teat, a procedure which al- lowed the milk to run from the milk cistern. W011 and -Humphrey (R6) reported that the first American patent on a continuous suction machine was obtained in 1860. Mattick gt_gl. (26) report that in 1836 Blurton, an Englishman, patented a cannula machine in which the pail was suspended from the cow. They also state that two British inventors, Hodges and Brockenden, in 1851, were probably the 2 first to use vacuum for removing milk from the udder, and. that Clovin of the United States extended the idea in 1860 to include a form of teat-cup. In 1895 and 1896 Shields of Glasgow took out patents for a device which he called a pulsator. His idea was to relieve the teats of constant vacuum. In 1897 Lawrence and Kennedy developed a milking machine in which Shields' principle of a pulsating vacuum was used, with a small motor situated on the pail lid to obtain this vacuum. In 1902 Hulbert and Park of New York patented a teat- cup with an inflatable lining divided into three sections. Mattick 32 El! (26) also reported that Gillies of Australia, in 1903, invented a double-chambered teat-cup to operate on vacuum alone and applied this teat-cup to the Lawrence- Kennedy machine which had been imported from Great Britain. The pulsator was now used to apply a squeeze to the teat by varying the pressure in the annular chamber instead of di- rectly interrupting the vacuum applied to the teat. With this combination the Lawrence-Kennedy-Gillies milking machine was develOped, the first to embody all the principles of machine milking as we know it today. Functions of the Milking Machine Mattick and others (26) state that the principle used by all modern milking machines to draw milk from the udder of a cow is, with few exceptions, basically the same as that established by the Lawrence—Kennedy—Gillies milker of 1903. This involves the application of a constant vacuum to the end of the teat to draw the milk out and convey it to a separate container, tOgether with a periodic squeeze applied externally to the teat in order to prevent congestion at the end of the teat. This is brought about by the principle of pressure differentials, the air pressure at the end of the teat being reduced so that the higher milk pressure within the teat will overcome the effects of the sphincter muscle and thus cause the milk to flow out. The milking machine in general is comprised of four main parts consisting of the vacuum pump, the teat cup as- sembly, the pulsator, and a vacuum regulator. In addition, the vacuum line, the milk conveying system, and the milk pail could be included as part of some specific milking system, but their functions need not be discussed in this thesis. The Vacuum Pump As described by Mattick and co—workers (26) and Noorlander (28), the purpose of the vacuum pump is to exhaust air continuously from the milking system in order to maintain the necessary vacuum level at the end of the teat for milk withdrawal, to operate the pulsator, and at the same time to maintain a constant level of vacuum throughout the system during the entire milking Operation. To Operate efficiently the pump must have reserve capacity. Mattick £2.3l: (26) and Noorlander (28) report that 5 the degree of vacuum in a milking system during operation is expressed as inches of mercury measured as a differential from atmospheric pressure and indicated by a conventional vacuum gauge. This differential is commonly referred to as the vacuum level. The Teat Cup Assembly Espe and Cannon (l8) and Mattick and co-authors (26) described the teat cup assembly as a cylindrical metal shell, containing a rubber liner shaped so that it makes an air- tight seal with the shell at the tOp and bottom, leaving a chamber between the shell wall and liner. The liner is open at the tOp into which the teat is inserted, and the bottom is attached to the milk receiver or teat cup claw. During the milking there is constant vacuum at the teat end of the liner. The Pulsator Mattick gt El. (26) and Noorlander (28, 29) have described the functions of the pulsator in detail. These authors state that the pulsator admits air into the chamber between the shell of the teat cup and the liner during one phase of pulsation and then permits the vacuum pump to re- move the air from this space during the next phase of the pulsation. This causes the liner to Open when vacuum is ap- plied and then to collapse when air is admitted, with the 6 open phase being the milking part of the cycle and the col- lapsed phase being the resting part. The purpose of this air rushing in several times a minute from the pulsator is to permit the liner to close with enough force and duration to reduce the vacuum on the outer teat surface, and to permit the liner to massage the teat. Constant vacuum without pulsation causes congestion at the end of the teat, but this massaging action maintains a reasonably normal blood circulation in the teat. The fre- quency of these cycles or pulsation rates depends on the make of the milking machine, and varies from LO to 120 pulsa- tions per minute. Most milking machine manufacturers recom- mend a specific pulsation rate for their machine. The speed at which milk is removed from the teat de- pends upon the time ratio between the open and closed phases of the complete pulsation cycle. The ratio of the times occupied by the two phases is called "pulsation ratio" or "milking ratio". This ratio may vary from 50:50 to about 75:25, which means that the teat cup liner is in the milking phase 50 or 75 percent of the cycle. Most pulsators in this country are designed for ratios from 50:50 to 60:h0. Several authorities, Mattick §t_§l, (26) and Noor- lander (28), agree that a milking ratio with a wide Open time of over 50 percent usually results in faster milking, but it may not provide enough closed time to permit prOper teat massage. The Vacuum Regulator The function of the vacuum regulator as described by Mattick gt_§13 (26) is to Open an air inlet valve in the vacuum line and allow air to enter the line when the vacuum exceeds the desired level. When the Operation of the milking machine permits air to enter the system, the vacuum decreases, the valve of the vacuum regulator closes, excluding the inlet of additional air until the desired vacuum level is obtained. The regulator can not prevent changes of vacuum, it can only operate to prevent a vacuum above a predetermined level. If an inadequate pump is used or if the vacuum line has too many air leaks, the regulator is not capable of making up lost vacuum; hence the system will not be Operating efficiently. To obtain the best results from the regulator, a pump with sufficient reserve capacity and of adequate size to handle a given number of milking units, must be used. A vacuum line free of air leaks is also essential. Weighing Devices One of the simplest methods of indicating milk flow rates is the use of the stop watch for recording the length of time the teat cups remain on the cow during milking. This method is limited, because the moment when the machine is re- moved from the cow will not often coincide with the end of milk flow. Where more accurate records are needed it is necessary to measure the rate at which milk is removed from the cow throughout the whole course of the milking period. This is done in a number of ways. As reported by Dodd SE 31: (12), Mattick and others (26), and Smith and Petersen (35), one method is to suspend a milking machine pail from a scale and take readings at frequent intervals--10, 15, or 30 seconds. This method probably has been the most used in the past. The individual quarter milking machine has been used as described by Baxter gt_gl, (3), Hall (22), and Matthews gt_§l. (27). This is a machine to deliver the milk from dif- ferent quarters through separate tubes to four compartments in a bucket or to four separate cylinders which are graduated in pound and one-tenth pound divisions. More recently the use of automatic recording equipment has been reported by Baxter (3), Whittlestone and Phillips (R3), and Hupp (25). One advantage of this procedure is that continuous recordings can be made without having strange ob- jects near the cows during milking. Ace §t_gl. (l), Gaines (l9), and Whittlestone (kl) have described other devices for recording milk flow rates. Factors Affecting Milk Flow Rates Day-tO-Day Variation Dodd (1h) gathered data on day-to-day variation in milk flow rates from lhl cows, with 326 records. The results indicated that day-to-day variations in the true measure of milking rate for a particular cow are relatively small as long as the milking routine is constant and correction is made for lactation trends. A.M. and P.M. Milking After studying the records of six cows for six consecu- tive days, Dodd (1R) found some variation between a.m. and p.m. milk flow rates. He states that while the rates in the mid-milking rate, peak flow, and machine rate were greater at the morning milking, the total time is longer because of the much higher yield. Hupp (25) found no significant differences in the rate of flow when 12 hours intervened between milkings, or when 11 or 13 hours intervened between milkings. Matthews §t_gl, (27) and Dodd and Foot (11) showed that if the differences in interval between milkings are great, such as 9- and 15-hour intervals, or 8 and 16 hours, differences in the rate of flow between the two milkings occur, the greater flow coming from the longer interval. Stage of Lactation In collecting data from R32 milkings of R8 cows during the early, middle, and late stages of lactation, Beck gt_gl. (R) found that both milk flow rate and the total time required to milk declined during the lactation period. Dodd's work (1R) also showed that the milking rate declines as lactation 10 advances, and that the decline is greater for fast milking cows. Hupp (2R) found that cows in their first lactation milked at a significantly faster rate as the lactation pro- gressed. The change in rate of flow during the lactation of cows in their second or subsequent lactations was not sig- nificant. In two consecutive lactations of R2 cows, Holthoff (23) reported that there was no increase in the rate of milk flow between the first and second lactation. Work done by Comberg and Zschommler (10) showed that milk flow rate was not affected by pregnancy or length of lactation. Variation Among Individual Cows Perhaps the most clear-cut finding has been that the rates of milking of individual cows show very wide differ- ences, even in self-contained herds with cows milked in the same manner. Some work done in England as reported by Mattick gt_gl. (26) showed wide differences in peak flow rates of individual cows at the same stage of lactation, giving similar milk yields. Elting's and LaMaster's work (16) showed that 7.0 per- cent of all cows required less than R.0 minutes, 81 percent required R.0 to 6.5 minutes, and 12 percent required over 6.5 minutes for the complete milking time. The minimum time 11 required for any cow was 3 minutes and 22 seconds, while the maximum time was 8 minutes and 17 seconds. Mattick and co-authors (26) reported on some data that were collected from 326 lactation records which illustrated that at the peak of lactation when the duration of milking is at its maximum, most cows take from R.0 to 8.0 minutes to milk. These cows were milked with lR inches of vacuum, a pulsation rate of 50 cycles per minute, and a pulsation ratio of 50:50. Petersen (30) and Beck (R) also found great variation in the rate of milking between cows. As reported by Hupp (25), Thoele found the differences in the rate of milking between cows to be significant at the one percent level. Variation Between Breeds Beck and his co-workers (R) conducted milk flow studies to determine the variation between breeds on the average rate of flow, maximum rate of flow, percent of yield obtained at 2.0 minutes, and machine time. They used a standard upright pail—type milking machine with 15 inches of vacuum and R8 pulsations per minute. One hundred and two cows of the Ayr- shire, Holstein, Guernsey, and Jersey breeds were used. Vari- ations in machine time ranged from 2.0 to 7.0 minutes, with an average of 3.5 minutes per cow. Seventybsix percent of the cows milked out in R.0 minutes or less, and 39 percent of the cows milked out in 3.0 minutes or less. The t-test was used to determine the significance of l2 differences between any two breeds for a given measurement. In average rate of flow the Holstein breed differed signifi- cantly from the other three breeds, exceeding the Jerseys about 27 percent, the Ayrshire about 35 percent, and the Guernseys about 65 percent. In maximum rate the Jerseys exceeded the Guernseys about 63 percent which was highly sig- nificant. The differences between the Jersey and Ayrshire breeds and between the Ayrshire and Guernsey breeds were not significant according to the t-test. In machine time the Holsteins and Jerseys were similar, with both breeds milking out significantly faster than the Ayrshires and Guernseys in that order. The breeds ranked in machine time, from fastest to slowest, as follows: Jersey, Holstein, Ayrshire, and Guernsey. Statistically significant variations between the Brown Swiss, Holstein, Guernsey, and Jersey breeds were found in rate of milking by Stewart 33 El, (38). Both the rate of milking and total milking time decreased significantly with advanced lactation. Inheritance There is some indication that milking rate is a her~ itable factor. Beck gt_gl. (R) reported that there were statistically significant differences in the speed of milking that could be attributed to the sire within a breed. Dodd and Foot (13) reported one sire with eight daughters, all of which were slower milkers than their dams; and another sire 13 with eight daughters whose rate of milking was very similar to their dams. They also reported significant correlations of 0.37 on R0 daughter-dam pairs and 0.37 on 35 pairs of sibs and half-sibs. Donald (15) reported that heritability of peak flow rate derived from Observations on paired singleborn cattle and twins was 85 percent. Repeatability was 0.8 - 0.9. Other workers, Stewart gt 31. (38) and Matthews 3E.Elf (27), also presented work which indicates that milking rate is a heritable factor. Variation Between Quarters Matthews §E_gl. (27) found that in many cows there were wide differences in the performance of the separate quarters of the udder. They observed 9R cows and found that R8.9 percent of these cows showed a difference of 20 percent or more between the quarters with the lowest percentage of its total yield milked out following the first 2.5 minutes of milking. In RO.R percent of the cows a difference of 0.6 pounds of milk per minute or more between the quarters with the highest peak flow rate and the quarters with the lowest peak flow rate was obtained. Smith and Petersen (35) and Comberg and Zschommler (10) also reported wide differences in the performance of separate quarters of the udder. Milking Rate and Yield Positive correlations between total milk yield and milking time (r=O.R7Rt0.0R2), and total milk yield and flow rate (r=0.7R6tLO25) were reported by Holthoff (23), but the correlation between flow rate and milking time (r=-0.097) was not statistically significant. Clough and Dodd (9) and Sandvick (32) have presented some evidence which indicated a relationship between milk yield and milking rate. This gives some strength to the belief that milk yield is dependent to some degree on the rate of milk removal from the udder. The Sphincter Muscle The sphincter muscles play a large part on the rate at which a certain amount of milk can pass out of the teat in a given time as reported by Mattick g3 31, (26), Whittlestone (R2), Gregoire (21), Smith and Petersen (35), Turner (R0), Stewart g£_§l. (39), and Gonokhov (20). Baxter and those working with him (3) conducted a study in which they milked the two hind quarters of four cows by teat cup and by teat cannula at three levels of vacuum--10.6, 15.9, and 20.R inches, using the individual, four cylinder type, quarter milking machine. The cannula was inserted into the ends of the teat and connected to the lid of one of the cylinders by the normal milk tube. During the experimental milkings the cannula was used on one hind quarter, while the 15 other three quarters were milked in the normal way with the teat-cups. This procedure made possible the study of flow rates at each level of vacuum through an orifice of a size determined by the bore of the cannula. When the eight quarters were milked by the teat-cup the maximum flow rates were significantly different. When the teat cannulae were used the eight quarters milked at nearly the same rate, sug- gesting that the teat orifice is a very important factor in controlling rate of milking. The maximum rate of milking by both the teat-cup and the teat cannula increased with the increase of the level of vacuum from 11 to 20 inches. The rate of increase was greater in the teat—cup than in the cannula milking. This suggests that the teat orifice was stretched Open to a greater degree at the higher levels of vacuum. Stewart gt_gl. (39) did some work with slow milking cows that gave support to Baxter's suggestion that higher levels of vacuum enlarge the teat orifice. Vacuum Levels and Pulsation Rates Smith and Petersen (3R) were the first to demonstrate that increased milking rate could be obtained with higher operating vacuums. They compared milk flow rates at 10, 12, 1R, and 16 inches of vacuum, with a pulsation rate of 50 cycles per minute for all observations. The differences of milk flow rate varied from 0.R3 pounds per 10 seconds for 10 inches of vacuum to 0.8R pounds per 10 seconds for 16 inches 16 of vacuum. These data when treated statistically show that the differences in milk flow rate between the different levels of negative pressure were highly significant. The work of Whittlestone and Verral (R2) did not agree with Smith's and Petersen's findings. However, a number of other workers, Stewart §t_§l: (39), Porter gt_g1: (31), and Bratlie (6), have shown that with higher levels of vacuum milk is withdrawn from the udder at a faster rate. Stewart and Schultz (39), working with slow milking cows, milked with the machine Operated at vacuum levels of 10, 12.5, and 15 inches of vacuum. They found that increasing the level of vacuum increased the speed of milking, and that the increase in milk flow rate was greater when they increased the vacuum from 10 to 12.5 than when they increased it from 12.5 to 15 inches. A standard single-unit, Sterling-type De Laval milker was used in this study. As noted by Smith and Petersen (35) the negative pres- sures developed on the nipple of a calf pail were invariably greater than the values obtained when the calves sucked cows. The maximum negative pressure develOped by sucking on the nipples ranged from 12 to 18 inches of vacuum as compared to a range of 9 to 16 inches when the calves sucked the cows. These authors suggested that the calf can develop sufficient negative pressure to overcome normal resistance of the teat sphincter. When they put the same calf on a cow known to be an easy milker and on one known to be a hard milker, they found that on the easy milker 10 inches of vacuum was needed 17 by the calf to withdraw the milk from the udder, whereas on the hard milker 16 inches was needed. It is often believed that fast pulsation speed in- creases the speed at which cows are milked. This is not the case unless the pulsation rate is so rapid that the liner does not fully collapse, and if this condition takes place damage to the teat is almost certain (26, 28). Stewart and Schultz (39) used pulsation rates of 20, 50, and 80 per minute and found that in general, increasing the pulsation rate significantly increased the speed of milking, but not as much as did increasing the vacuum level. The effect of increasing pulsation rate was most marked when increasing it from 20 to 50. No noticeable effect occurred on increasing the rate from 50 to 80. Clough at £1. (7) studied the results on milk flow rates when using three different pulsation rates. They used rates of 20, 50, and 80 pulsations per minute, with a vacuum of 15.2 inches and a 50:50 ratio. Their data indicate that the increase in pulsation rate gave faster milking, and that all the effects were significant at the five percent level. They stated that the faster the pulsation the faster will be the milk-flow at the time of maximum let-down, but it is pos- sible that it will be less efficient than a slower pulsation at the end of let-down when the internal pressure has drOpped and the teats do not fill so readily with milk. Bratlie gt 31. (5) divided 16 cows into two similar groups and used a pulsation rate of R0 for a control while 18 pulsation rates of 60 for one trial and 75 for a second trial were used on the experimental cows. The experimental cows produced R.8 percent less milk than the control cows, and the difference was significant. The milking time was slightly longer for the experimental cows, but the peak flow rate was similar for both groups. There was some indication of ad- verse effects on the teats from the higher pulsation rates, also high cell counts were more frequent in the experimental group than in the control cows. Pulsation Ratios and Vacuum Levels The first experimental work done to observe the effect of variation in pulsation ratio on milk flow rates was done by Smith and Petersen (36). They found that widening the pulsation ratio increased the rate of milk flow, but they did not consider this increase of any great importance. Raising the pulsation ratio from that of 50:50 to 60:R0 (60 percent milking) and 75:25 gave a slight increase in milk flow rate. Widening the milking ratio increased the rate of milk flow but not in prOportion to the increased time of vacuum application. The 75:25 ratio had a tendency to in- crease teat congestion. A more recent study has been made by the National Institute for Research in Dairying and reported by Mattick and others (26). They milked a group of cows at 15 inches of vacuum with varying pulsation rates and ratios. The milking rate increased over 30 percent when the pulsation 19 rate was increased from R0 to 160 per minute at a pulsation ratio of 1:1. With a pulsation rate of R0, the milking rate was increased by 23 percent when the pulsation ratio was changed from 1:1 to 2:1, and 3R percent when the pulsation ratio was changed from 1:1 to 3:1. When the pulsation rate was set for 160 cycles per minute there was an increase in milking rate of R0 percent when the pulsation ratio was changed from 1:1 to 3:1. Average yield and milking time of a group of cows milked at 15 inches of vacuum and 60 pulsations per minute with ratios of R:1 and 1:1 at intervals of 12 and 12 hours or 1R and 10 hours were compared. With each milking interval milking time was reduced when the pulsation ratio was changed from 1:1 to R:1. The milking time of slow-milking cows was also decreased. Changing the pulsation rate and widening the ratio enabled Ardran g£_§l: (2) and Clough and Dodd (8) to increase the maximum milking rates. Wilson (R5) reported that when the pulsation ratio was widened from 1:1 to R:1 there was a greater increase in peak milk flow than that obtained by an increase of vacuum from 13 to 25 inches. Effects of Stimulation Before Milking Higher rates of milk removal were obtained by Smith and Petersen (37) when cows were stimulated before milkinG. Hupp (25) reported that the maximum rate of flow was significantly faster if the teat-cups were put on the teats 20 immediately after preparation. It has been suggested by Whittlestone (AU) and Turner (R0) that the reason for some cows being slow milkers was due to insufficient let-down of milk, but Hupp (25) injected a group of cows with oxytocin and found that it had no ef- fect on rate of milk flow with average and fast milking cows. EXPERIMENTAL PROCEDURE All the cows that were housed in one of the dairy barns of Michigan State University were used in this experi— ment. At the beginning of this study the milking string consisted of a group of 65 cows in different stages of lac- tation. During the experimental period some cows ended their lactations, others started their lactations, some were culled from the herd, a few develOped some adverse udder conditions, and data were not collected from a few cows due to some ir- regularities in communications. Because of these factors it was not possible to collect data from 31 cows during the entire experimental period, and it was deemed necessary to drOp these from the experiment. This left a total of 3R cows in the final analysis, consisting of 23 Holstein, 6 Brown Swiss, and 5 Jerseys. The cows were milked in tie-stalls by one man Oper- ating three milker units of a De Laval pipeline milking system. The man operating the milkers also prepared the cows before milking. The udders Of the cows were washed with warm chlorinated water, massaged to facilitate the prOper let-down, and checked with a strip—cup for mastitis. In most cases the teat cups were placed on the teats within two minutes after udder preparation. As far as possible the cows were milked in regular order. 21 22 In this study the De Laval Magnetic milking machines were used, with some alterations made in order to obtain the proper pulsation ratios desired. The milking machines were Operated with 12.5 inches of vacuum and a pulsation rate of R8 cycles per minute. The De Laval 05 liners were used during the entire experiment. Pulsation Ratios In this experiment the average milk flow rate and the peak milk flow rate for three different pulsation ratios (the milking to rest ratios) were studied. With two of these ratios, a 50:50 ratio and a 6R:36 ratio, the entire udder was milked with the same ratio. The third ratio was a vari- able ratio using 6R:36R (R indicates rear) for the rear half and a 36:6RF (F indicates front) for the front half. Since on the average 60 percent of the milk is produced by the two rear quarters and R0 percent by the two front quarters, this third ratio was used with the hope that greater flow rates would be obtained. In this thesis the third ratio will be referred to as the 6R:36R - 36:6RF ratio. The desired pulsation ratios were obtained by modifying the standard De Laval timer-converter, as shown in Figure l. The mercoid switch had been removed and replaced with a ro- tating cam. The cam rotated at the speed of the electric motor in the timer—converter, in this case approximately R8 revolutions per minute. This caused the milker to milk at a constant pulsation rate of R8 pulsations per minute. Figure 1. The modified De Laval timer-converter used for obtaining pulsation ratios. 23 2h The rotating cam activated a micro switch by means of an arm which pressed against the perimeter of the cam. When the raised portion of the cam pressed against the arm, the arm was moved upward so that the micro switch was closed and a current was allowed to pass through the switch and to the solinoid on the stall cock. When the lowered portion of the cam was pressed against the arm, the arm was lowered and the micro switch Opened, thus stopping the flow of current to the solinoid at the stall cock. The solinoid on the stall cock Operated a valve which when raised would admit atmospheric air to the outer chamber of the teat cup. When the valve was lowered the outer cham- ber of the teat cup would be opened to the vacuum line and the air would be pumped out of the outer chamber by the vacuum pump. When the electrical current was passed through the solinoid, the valve was raised. When the current was stopped, the valve was lowered. Therefore the rest phase of the pulsa- tion corresponding to the length of time current was passed through the solinoid, which in turn was the length of time the micro switch was closed. The length of time the micro switch was closed in re— lation to the length of time it was open was determined by the portion of the cam which was raised in relation to the portion which was not raised. The cam was so constructed that the raised portion could be adjusted to any particular pulsation ratio desired, 25 within certain limits. The cam was composed of two discs, each of which had a raised portion. The discs could be adjusted so that the raised portion of the entire cam could be lengthened or shortened. It was by changing the length of the raised por- tion of the cam that the various pulsation ratios were ob- tained. The vacuum recorder, as described by Noorlander (28), was used to check the setting of the timer-converter in order that the desired pulsation ratio could be obtained. The 50:50 pulsation ratio on all four quarters was obtained by setting the timer-converter for a 50:50 ratio, and by using the standard De Laval milker claw and pulsator. To obtain the 6R:36 pulsation ratio on all fOur quarters the timer- converter was set for a 6R:36 ratio, one air port of the standard De Laval claw and pulsator was closed, and a single air hose was used to connect the milker claw and pulsator. Figure 2 shows the converted claw, and Figure 3 shows the converted pulsator. The 6R:36R - 36:6RF pulsation ratio was Obtained by setting the timer-converter for a 6R:36 ratio and using the standard pulsator with a double air hose. The air ports in the milker claw were by-passed by brazing three-eighths inch copper tubing together in such a way, as shown in Figures R and 5, that a front to rear milking action could be had. A hole was drilled midway in a three inch piece of OOpper tubing, and to this drilled hole another three inch piece of Figure 2. 26 The converted standard De Laval milker claw used to Obtain the 6R:36 pulSation ratio. Figure 3. The converted standard De Laval pulsator used to obtain the 6R:36 pulsation ratio. 27 Figure R. A device used to by-pass the air ports of the standard De Laval milker claw. 28 Figure 5. A device attached to the standard De Laval milker claw, used to by-pass the air ports. 29 30 tubing was brazed, making a tee. Two tees were made with the stem of one being an inch shorter than the other, the shorter tee being brazed to the tOp side of the longer one in such a way that the ends of the stems were in line vertically. A clip was brazed to this double tee so it could be fastened to the milker claw. By attaching the double air hose from the pulsator to the stem ends of this device and making sure that the cross ends of the top tee were attached by single air hose to the teat cups for the two front quarters, and the cross ends of the bottom tee were attached to the teat cups for the two rear quarters, a front to rear milking action was obtained. With the above device in place and the timer—converter set for a 6R:36 ratio, one side of the double air hose oper- ated on a 6R:36 ratio, and the other side operated on a 36:6R ratio. The vacuum recorder was used to indicate which side of the double hose operated on the 6R:36 ratio, and this side was attached to the bottom stem of the tee that Operated the teat cups for the two rear quarters. This gave a 6R:36 ratio to the two rear quarters, and a 36:6R ratio to the two front quarters. The stem end of the lower tee was painted red as was also the 6R:36 side of the double air hose so that no mix-up would be made during the assembling of the machines. The equipment used for the three ratios mentioned above was very similar to standard milking equipment and did not appear as strange objects near the cows during milking. The milking routine ran just as smoothly as it did with the 31 standard equipment. In all three ratios the milk was carried from the milker claw to the pipe line in the usual way. The Recording Milk-O-Meter In this experiment the recording Milk-O-Meter as de- scribed by Hupp (25) was used to collect milk flow data. The Operating parts of this meter are the same as in the regular Milk-O-Meter, except that two electrical contacts have been added so that each time the weigh tray trips, an electrical impulse is sent to the recorder. This impulse from the meter is carried by a wire which is plugged into a jack. One jack is located behind each.Milk-O-Meter mount in the barn. These jacks are connected to a low voltage wire which goes all the way around the barn and leads to the recorder. Each time the recorder receives an impulse from the meter, it makes a mark with an indelible marker on a tape which is driven past the marker at a constant speed. The recording meter can be used for any cow in the milking line Of the barn with the recorder located in one place. Procedure for Collecting Data Since it has been fairly well established that with equal intervals between milkings there is very little dif- ference between a.m. and p.m. milk yields (11, 25, 27, 33), data were collected from the evening milkings only for this experiment. 32 Only one recording Milk-O-Meter could be used, so data were collected from one-third of the herd each day. During a period of nine days three observations were made from each cow with two days between each observation. A two—week period divided into two parts was used for each pulsation ratio. Four days were allowed for an adjust- ment of the cows to the new ratio, while the second part con- sisted of the nine days in which the data were collected. Each treatment was repeated once, but in a different order as shown in Table I. This gave a total of six obser4 vations from each cow for each treatment except for the 6R:36R - 36:6RF ratio. While collecting data on the 6R:36R - TABLE I The Order in Which Data Were Collected For Three Pulsation Ratios Treatment Pulsation Ratio Number No. Observations of Weeks Per Cow I 50:50 Entire Udder 2 3 II 6R:36 Entire Udder 2 3 III 6 :36 Rear Quarters 3 :6R Front Quarters 2 3 II 6R:36 Entire Udder 2 3 III 6R:36 Rear Quarters 36:6R Front Quarters 2 O I 50:50 Entire Udder 2 3 33 36:6RF ratio the second time, a number of the cows became very nervous and a few developed adverse udder conditions. Because of this the treatment was discontinued. This left a total of only three observations for this ratio. Milking Rates In addition to the graphic record made of each milking by the recorder, a stop watch was used to obtain the total milking time for each cow, this time being taken from the time the teat cups were placed on the teats until they were removed. The total pounds of milk produced were taken from the tape in the Milk-O-Meter and the average flow rate for each cow was determined by dividing the total pounds of milk produced by the total milking time.‘ The data used to determine peak flow rates were ob- tained from the recording Milk-O-Meter. The Milk—O-ueter records in quarter-pound increments, and each tine the re- corder received an impulse from the meter it made a mark with an indelible marker on a tape that was driven past the marker at a constant speed. Figure 6 shows a tape that was used with the recording Milk-O-Meter. The distance between each indelible mark represents one—quarter of a pound. Each division on the tape equals a second, with 15 divisions to the inch. Since all the cows for one milking were placed on one long tape, a pencil line was placed across the tape and the number of the cow written in to show when the start and the end of milking took place for each cow. Figure 6. A graphic recording tape used with the recording Milk-O-Meter. 3A 0000;. WI. ] l::l:n.l.zl.,:li'sia =1 'lmzlszul.than] i. ; 35 The record on each cow was checked in several places on the tape in order to find the greatest flow rate. In the area of the greatest flow rate five pounds of milk were marked off and the number of seconds or minutes were re- corded that covered this area. These data were converted into pounds per minute for peak flow rates. In two cases the milk yield was not large enough to mark Off five pounds for the greatest flow rate, so two pounds of milk were used in place of five. A simple device shown in Figure 7 was constructed to facilitate the reading of the tapes. The roll of tape was placed on the roller at one end and stretched over the board and attached to the roller at the other end. By turning the handle the tape could be rolled out over the board and read. The Milk Quality Test There were some changes in the regular milking routine in that a small quantity of milk was removed from each quar- ter before udder preparation and after udder preparation, a composite sample was taken with the Milk-O-Meter butterfat sampler, and some strippings were taken after the completion of milking. These samples were used to determine the effect of the above ratios on udder irritation, which was determined by the use of the Milk Quality Test, with some alterations, as described by Noorlander (28). Figure 7. A device used to facilitate the reading of the graphic tapes. 36 37 The Milk Quality Test is a screening device for de- tecting abnormal milk due to irritation in the udder. Chem- icals in the reagent that is used in the Milk Quality Test condense or thicken the protein found in the white blood cells or in the cytoplasm of damaged tissue. The degree of thickening or viscosity will show the concentration of these white blood cells in the milk. The geometric average cell count from milk of healthy cows has been found to be 70,000 per millimeter. Any thickening that persists will indicate over 500,000 body cells per millimeter of milk, and is an indication of udder irritation. When the Milk Quality Test is applied to abnormal milk the reaction due to the reagent starts to thicken when the leucocyte count exceeds 250,000 per millimeter, and becomes a very thick viscous gel at 5,000,000 cells per millimeter. The quality of the milk is scored according to.the thickness of this reaction. No re- action is represented by a score of "O" and indicates no irritation, a score of 1 represents a slight thickening or cell count of about 500,000 cells per millimeter, a score of 2 indicates a medium thickening with a cell count of 2,500,000, a score of 3 represents a very thick gel and an indication of 5,000,000 or more cells per millimeter. The irritation study was a separate project being car- ried on simultaneously by another investigator. RESULTS AND DISCUSSION In Table II the data are presented showing the effects of three different pulsation rates on the average flow rate TABLE II Average Flow Rates and Peak Flow Rates Obtained From Three Pulsation Ratios Part A Pulsation Ratios* I II III I II III Average Flow Rate Peak Flow Rate lbs./min. lbs./min. Cow No. Brown Swiss 3078 2.85 R.25 3.86 R.36 R.80 5.20 30R7 R.56 R.85 R.00 7.09 7.28 7.23 3068 3.R7 3.03 2.12 5.66 6.32 6.55 3008 5.31 6.23 5.12 6.5R 7.73 6.22 3OR8 3.56 3.31 2.9R R.66 R.96 R.39 3077 3.85 3.52 3.18 5.11 5.91 5.11 Mean 3.93 R.20 3.5R 5.57 6.17 5.78 1157 3.80 3.35 3.20 6.10 6.57 6.71 1181 2.51 2.53 2.20 R.7l 5.71 R.7O 118R 2.13 2.38 1.86 3.30 R.O2 3.53 1185 1.97 2.00 2.26 3.8R R.5R 3.66 11R9 2.98 3.21 3.22 5.8R 6.67 5.90 Mean 2.68 2.69 2.55 R.76 5.50 R.90 *Pulsation Ratios: I--A 50:50 ratio on the entire udder. II--A 6R:36 ratio on the entire udder. III--A 6R:36 ratio on the rear half and a 36:6R ratio on the front half of the udder. 38 TABLE II--Continued Part B 39 Pulsation Ratiosfi I II III I II III Average Flow Rate Peak Flow Rate lbs./min. lbs./min. Cow NO. Holstein 609 5.08 5.35 R.90 7.RS 7.78 7.20 626 3.R8 R.Rl R.OO R.72 6.12 5.59 572 2.R5 2.81 3.18 5.13 6.21 5.21 596 5.19 5.8R 6.22 9.15 10.96 10.25 638 2.30 3.RR 2.3R 3.10 3.R7 3.16 686 3.50 R.R7 3.58 6.96 8.86 7.17 A110 3.0R 3.R0 3.0R 6.20 7.52 7.38 630 3.R6 3.97 3.16 R.38 5.22 R.21 A 78 2.22 2.01 2.10 7.08 8.6R 5.97 5R2 R.l7 3.50 R.31 8.33 8.85 8.69 685 3.RR R.00 2.72 R.R9 5.Rl R.76 '6R0 3.53 3.16 2.5R 5.82 6.32 5.81 608 5.59 R.9l 5.30 7.69 9.03 8.67 6R3 R.5l R.R6 R.78 7.81 9.22 8.37 6R9 R.3O R.26 3.8R 5.56 6.89 5.73 612 5.69 6.5R 6.08 7.R6 8.69 8.0R 598 3.65 R-l7 3.50 5.89 7~R9 7.73 K302 R.1O R.01 5.16 6.21 7.61 6.97 6RR 2.65 3.3R 2.6R 3.68 R.39 3.86 606 5.16 6.10 R.5R 8.R9 9.99 9.09 693 1.9R 2.36 2.06 2.50 2.88 2.77 6R1 R.6l R.98 3.58 6.88 7.31 6.RO 63R 2.70 2.93 2.9R R.91 5.91 5.17 Mean 3.77 R.ll 3.76 6.51 7.16 6.RR fiPulsation Ratios: I--A 50:50 ratio on the entire udder. II—-A 6R:36 ratio on the entire udder. III--A 6R:36 ratio on the rear half and a 36:6R ratio on the front half of the udder. R0 and the peak flow rate for the individual cow, and the mean rate for each breed. Averagg Flow Rate The results of this experiment indicate a slightly larger average milk flow rate as shown in Table III when the 6R:36 pulsation ratio was used, with the 50:50 ratio and the 6R:36R - 36:6RF ratio following in that order. Within the Holstein breed the average milk flow rate was R.1l pounds per minute when the 6R:36 pulsation ratio was used, as compared with 3.77 pounds per minute for the 50:50 ratio, and 3.76 pounds per minute for the 6R:36R - 36:6RF ratio. With the Brown Swiss breed R.2O pounds per minute were obtained from the 6R:36 ratio, 3.93 pounds per minute from the 50:50 ratio, and 3.5R pounds from the 6R:36R - 36:6RF ratio. .The average milk flow rate within the Jersey breed was as follows: 2.69 pounds per minute with the 6R:36 ratio, 2.68 pounds per minute with the 50:50 ratio, and 2.55 pounds per minute with the 6R:36R - 36:6RF ratio. The mean for all breeds follow the same pattern: 3.91 pounds per minute with the 6R:36 ratio, 3.6R pounds per minute with the 50:50 ratio, and 3.5R pounds per minute with the 6R:36R - 36:6RF ratio. Analysis Of variance shows significant differences (P>0.0l) between the breeds for the average rate of milk flow, thus verifying the work done by Beck and his co- workers (R). The difference between the ratios is not sig- nificant. Rl TABLE III The Mean Differences in Average Flow Rate and Peak Flow Rate For Three Dairy Breeds Pulsation Ratiosfi I II III I II III Average Flow Rate Peak Flow Rate —-lbs./min. --—lbs./min.-- Breed No. of Cows Holstein 23 3.77 R.ll 3.76 6.51 7.16 6.RR Brown Swiss 6 3.93 R.20 3.5R 5.57 6.17 5.78 Jersey 5 2.68 2.69 2.55 R.76 5.50 R.9O All Breeds 3R 3.6R 3.91 3.5R 5.80 6.7R 6.10 *Pulsation Ratios: I--A 50:50 ratio on the entire udder. II--A 6R:36 ratio on the entire udder. III--A 6R:36 ratio on the rear half and a 36:6R ratio on the front half of the udder. When the Brown Swiss cows were milked with the 50:50 ratio and with the 6R:36 ratio their flow rates exceeded the Holstein and Jersey breeds in that order. The Holstein breed exceeded the Brown Swiss and Jersey breeds in that order when milked with the 6R:36R - 36:6RF ratio as shown in Table III. Peak Flow Rate The results obtained for the peak flow rates do not follow the same pattern as those for the average milk flow rates. With the Holstein breed, differences between the AZ pulsation ratios are similar to those of the average milk flow rates. The 6h:36 ratio gave the highest peak flow, 7.16 pounds per minute, with the 50:50 ratio giving the next to the highest peak flow rate, 6.51 pounds per minute, and the 6h:36R - 36:6hF ratio giving the lowest peak flow rate, 6.uu pounds per minute. However, as shown in Table III, with both the Brown Swiss and the Jersey breeds the 6h:36R - 36:6HF ratio gave the next to the highest peak flow rate, 5.78 pounds per minute for the Brown Swiss and b.90 pounds per minute for the Jerseys. In both the Brown Swiss and Jersey breeds the 6u:36 ratio gave the highest peak flow rate, 6.17 pounds per minute for the Brown Swiss, and 5.50 pounds per minute for the Jerseys. The 50:50 ratio gave the lowest peak flow rate for the Brown Swiss and Jersey breeds, 5.57 pounds per minute for the Brown Swiss, and n.76 pounds per minute for the Jersey. No precise explanation can be given for this difference. The mean rate for all breeds follows the same pattern as that for the Brown Swiss and Jersey breeds, with the 64:36 ratio exceeding the 6A:36R - 36:6uF ratio and the 50:50 ratio in that order. The peak flow rates for all breeds are as follows: 6.7a pounds per minute for the 6h:36 ratio, 6.10 pounds per minute for the 6h:36R - 36:6MF ratio, and 5.80 pounds per minute for the 50:50 ratio. As with the average milk flow rates, analysis of vari- ance shows a significant difference (P>0.0l) between the breeds for peak flow rates. The difference between the #3 ratios approaches significance at the five percent level for the peak flow rates. TABLE IV The Average Milk Yield Per Milking and Machine Time As Obtained From Three Pulsation Ratios Part A Pulsation Ratios* I II III I II III Milk Yield Machine Time II lbs. per milking -———-—minutes Cow No. Brown Swiss 3078 17 2h 26 6.1 6.0 6.6 30u7 30 29 26 6.6 6.0 6.3 3068 1h 13 13 u.3 u.u 5.5 3008 30 32 30 5.7 5.6 5.8 30MB 25 23 2a 7.0 7.5 8.2 3077 18 19 18 u.7 5.3 5.7 Mean 22 23 23 5.7 5.8 6.u Jersey 1157 l7 l6 l7 u.6 u.8 5.u 1181 9 9 9 3.6 3.7 h-l 118A 9 9 9 u.3 3.8 u.7 1185 9 10 10 u.5 5.0 u.3 11u9 15 16 15 5.1 5.0 u.7 Mean l2 l2 l2 u.u u.5 u.6 *Pulsation Ratios: I--A 50:50 ratio on the entire udder. II--A 6uz36 ratio on the entire udder. III--A 6h:36 ratio on the rear half and a 36:6u ratio on the front half of the udder. TABLE IV--Continued Part B Pulsation Ratiosfi I II III I II III Milk Yield Machine Time lbs. per milking minutes Cow No. Holstein 609 30 31 30 6.0 5.7 6.2 626 21 22 23 6.1 8.9 5.8 572 17 17 17 6.8 6.8 5.7 596 27 27 30 5.5 11$? 8.8 638 20 23 20 9.1 9.2 9.2 686 17 19 18 8.9 8.8 5.1 A110 15 16 17 5.2 5.5 5.5 630 19 20 18 5.6 5.1 6.3 A 78 10 10 11 8.7 5.1 5.0 582 16 16 16 3.9 8.6 3.9 685 20 20 20 5.9 5.1 6.1 680 16 17 16 8.9 5.3 6.2 608 27 29 32 5.0 6.0 6.0 683 21 21 23 8.6 8.8 8.8 689 26 211 23 5.3 5.6 6.0 612 38 33 35 6.1 5.1 5.7 598 19 22 23 5-7 5.3 6.5 K302 23 22 26 5.8 5.3 5.6 688 18 18 18 6.8 5.5 6.9 606 28 25 28 8.7 8.1 5.8 693 15 15 16 8.0 6.5 7.5 681 20 22 20 8.8 8.8 6.1 638 13 13 13 5.1 8.6 8.2 Mean 20 21 21 5.7 5.8 5.9 *Pulsation Ratios: I—-A 50:50 ratio on the entire udder. II-—A 68:36 ratio on the entire udder. III--A 68:36 ratio on the rear half and a 36:68 ratio on the front half of the udder. L15 Machine Time The results for machine time and milk yield for each cow and the individual breeds, are presented in Table IV. As presented in Table V there is very little dif- ference between the ratios in machine time. In all three ratios the Jersey breed required the least time to milk out, but the milk yields for the Jerseys in all three ratios were much lower than those for the Holstein and Brown Swiss, a fact which may account for the lower machine time. TABLE V The Average Milk Yield and Machine Time By Breed As Obtained From Three Pulsation Ratios Pulsation Ratios* I II III I II III Milk Yields Machine Time Ibs. per milking minutes Breed No. of Cows Holstein 23 20 21 21 5.7 5.8 5.9 Brown Swiss 6 22 23 23 5.7 5.8 6.8 Jersey 5 12 12 12 8.8 8.5 8.6 All Breeds 38 19 20 20 5.5 5.3 5.8 *Pulsation Ratios: I-—A 50:50 ratio on the entire udder. II--A 68:36 ratio on the entire udder. III--A 68:36 ratio on the rear half and a 36:68 ratio on the front half of the udder. 86 TABLE VI The High, Low, and Average Machine Time for the Holstein, Brown Swiss and Jersey Breeds Pulsation Ratios* I II III minutes Holstein High 9.1 9.2 9.2 LOW 309 he]. 3'9 Average 5.7 5.8 5.9 Brown Swiss High 7.0 7.5 8.2 LOW 14.03 “-0,-6- 5.5 Average 5.7 5.8 6.8 Jersey High 5.1 5.0 5.8 Low 3.6 3.7 8.1 Average 8.8 8.5 8.6 All Breeds High 9.1 9.2 9.2 LOW 306 307 3'9 Average 5.5 5.3 5.8 *Pulsation Ratios: I--A 50:50 ratio on the entire udder. II--A 68:36 ratio on the entire udder. III--A 68:36 ratio on the rear half and a 36:68 ratio on the front half of the udder. As shown in Table VI, variation in machine time for all cows and all three ratios range from 9.2 to 3.6 minutes, with an average of 5.6 minutes per cow. Cow Number 638, known to be a hard milker, has the highest machine time in all three ratios as shown in Table IV. When the 50:50 ratio was applied, a Holstein cow LL? required the longest time to milk out, 9.1 minutes, and a Jersey cow required the least, 3.6 minutes. This was also the case with the 68:36 ratio; a Holstein cow required 9.2 minutes to milk out, whereas a Jersey cow required only 3.7 minutes to milk out. For the 68:36R - 36:68F ratio the highest and lowest machine times were obtained from two cows of the Holstein breed, with a high of 9.2 minutes and a low of 3.9 minutes. When comparing the ratios for the percentage of cows milked out in 5.0 minutes or less and 8.0 minutes or less, it was found that for the 50:50 ratio and the 68:36 ratio 81 percent of the cows were milked out in 5.0 minutes or less, and 6 percent were milked out in 8.0 minutes or less. For the 68:36R - 36:68F ratio 26 percent of the cows milked out in 5.0 minutes or less, and 3.0 percent in 8.0 minutes or less. When considering all the cows and all three ratios together, 36 percent of the cows milked out in 5.0 minutes or less and 5.0 percent in 8.0 minutes or less. Udder Irritation As mentioned above, while applying the 68:36R - 36:68F ratio the second time, some adverse udder conditions were noticed with some cows, while a number of the cows became very nervous and would move back and forth while being milked. In a few individual cases it appeared that the cows held up their milk. O 88 It is difficult to know how these conditions came about. There is a possibility that either the front or rear quarters were over-milked causing udder irritation and general discomfort to the cow. If this were the case, these conditions might have been avoided if two different pulsa- tors had been used, one to control the rear quarters and one to control the front quarters. With separate pulsators the teat-cups on the rear quarters can be in Operation while those on the front quarters are inactive, or vice versa. There is also a possibility that irritation started with a previous treatment may have reached its peak during the second replica of this ratio, causing general discomfort and irritation to the udder. The Milk Quality Test results show the least irrita- tion with the use of the 68:36 pulsation ratio, and the greatest irritation with the 50:50 pulsation ratio. SUMMARY Comparisons were made of the effects of three dif- ferent pulsation ratios on the average milk flow rate and peak flow rate. These pulsation ratios consisted of a 50:50 ratio and a 68:36 ratio which were applied to the entire udder, while the third was a variable ratio using a 68:36R (R indicates rear) ratio for the rear half, and a 36:68F (F indicates front) ratio for the front half of the udder. Thirty-four cows, consisting of 23 Holstein, 6 Brown Swiss, and 5 Jerseys, were used in this study. Data were collected from these cows during the evening milkings. For the 50:50 and 68:36 pulsation ratio six observations were made on each cow. Some adverse udder conditions arose during the second replica of the 68:36R - 36:68F pulsation ratio, and because of this, only three observations were made on each cow for this ratio. A timer-converter was develOped so that the three desired pulsation ratios could be obtained. The standard De Laval milker claw and pulsator were adapted to fit the de- sired ratios obtained by the timer-converter. A vacuum re- corder was used to check the setting of the timer-converter to be sure that the proper pulsation ratios were obtained. The recording Milk-O-Meter was used for collecting the data on milk flow rates and machine time. The Milk Quality Test was used with each pulsation ratio to detect any irritation that might occur in the udder. 119 CONCLUSIONS From this study the results indicate that a slightly higher average flow rate and peak flow rate were procured when the 68:36 ratio was used as compared with the 50:50 ratio and the 68:36R - 36:68F ratio. The 50:50 ratio gave a slightly higher average flow rate than that obtained by the 68:36R - 36:68F ratio. With the Brown Swiss and Jersey breeds the 68:36R - 36:68F ratio showed a slightly higher peak flow rate than that of the 50:50 ratio. For the Holsteins the ratios ran from highest to lowest in peak flow rates in the following order: the 68:36 ratio, the 50:50 ratio, and the 68:36R - 36:68F ratio. Analysis of variance showed a highly significant dif- ference between breeds for both average milk flow rate and peak flow rate. There were no significant differences be- tween the ratios. 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