WWI\lHlHl!"HH|WNW

I
»
~

145
926
HTHS

 

WEE FLUORGMETREC DETERMINATION OF
0-9HTHALIC ACES iNCLUDéRG THE
ASSEMBLY OF A FLUQROMETER

Thesis for flu Degree of M. S.
MICHEGAN STATE COLLEGE
Gian A flaemmes
1954

THE twmiwflm r: 5- araflarma a Mflwim £013)
mwzma mg .132me1 (I A mam-mm

8v

'

Glen is . Thomas:

In. THE-SIB

thumwmldWMIotmm
M Coll-a- “ Lana-am and Appllod acum-
139ml Walnut a! that W
for th- aws-u at

warm (5' $12535

dew

1931:

0x

\M ..
L

Acmmnm

nuaMMrdeIunmmuuuumhunr
Wnlmuk.wuimrfwm
mmommmmmp
«aim-unnufwunun.

EMU-UH"
fliflw
HIV.“
"fl

3440.92

TABLE} OF C33333

9363
BIJTWICLIuooooaoooucuoounvoocoocu...no"oucooosou.ccaonotcunooooo-o
“ATM a maamoo¢oonuooo00......000000.00.0.0..-"moo-0000390..

WAOOOOOCOOCOOOOOQOCCOOOOOOOOOOO.OOOOOOCIOCOOOOOOOOOOO.0C...

1. 0.53381 Wcc000000.00.coon-cc:cocooooooooooooooooouoci
2. Immntatim 8M F's-1‘9? 301931510500...cocoon-000.000.000.000

0 Hf.“ 9‘ I“ 93 3‘31. FEWOOOCQGOOCOOOQQOOOOOOOOQto...

c £1,130“ at P“ an m Fwwvme.00....":uocoooooooooooouu

So u” .I cum“ EWIO00000000000000.0000.0000noaoooobt.
6. The Effect of Varying Quantum) af Sulfuric Acid Upon the

Wit? 0‘ nwemOIOOOOOOIIOOOCOCOQOOOOOOOOOOOOOOOOOOOQ

1. Detonaimtion 0f Opt-1M Emotion Tim-00000000000000.ooniooaoo

6. Duns-“"193 0‘ 09% m Immooo.oco¢ocncoooo¢no...

9. Stability at Fluorescent 51-93138 in 2 E Sm Hych‘ozicm.......

10. infirm“ 0‘ u}. Cdibration mOOOOQQOOIOOoaooooooc.oooooo

11. fippncltion of that {lemma 1n the Mama at Various

cam“.“.000tOOOOOOOOOCOOOOOIO000.000....OOGOOOODIOQOIOO
”0 kW Woo..-oocooosoooto...ooocouconoooovooootooo

35:55 coca-acne." :- u P

23%

M
t“

g
WOO...OIOOOOO....00....0000COC£5OOOOOSII.OOCOOOOOCOOCOIOOOOOOOO

mmm cum......gononooaooogooOca-oonocnoooooooo.no¢ooono...coo 2.;
11"! ‘ ’
Aflb£mn....ggoooooocco-ooaooo000930.00.oonooowo-oooooooococoon-00.66 2‘3

fiIffl'CSICJKL

I!» continuation of 1,2-diccboay1in acids with react-Gina to yield
' dyu 03 th- nmmin type has been record”! by Foigl (1) , and the
fluorescent ooapoundl found, no I "cult of than condomstionn, hm
bun was for qualiutin identification of lfiodicarbowlio acid

Ctr-newt“. Ibo "action, according to 10151, proceed: at fallow“

OH
(3'33! 3
2 (I! o 0001! —’§2§—) coon
C
/
O 0 on
O// O
I (nummoin)
.COOH . ’
N573 A 000. i‘
c r O 3
’°
. OH
0,, c. 0‘) m
I!

 

.0

1'. smug punish-filler that-loom I'Olultl on the addition of sum
to tho auction product (I) an. to tn? tor-man at tho ionic an (n).
this anon-«no. il indicative of tho pro-om. of the 1,2-diearbovlio
laid Matti”.

Rm (2) ha and. a mmtitatin application of this type of rotation
in devolving a fluomotrio method foi- thn autumnaiion of lucoinie acid

llama-m. mmmiuzsmmwmm.
deWnWdeluumoumg-u.

an: m or raw

mwmmmamudwgwnmmmmamm
of W difficulty. Th 43th of 813011 mixture: usually
inwlm mm «ecu-epic walk-£11m (3, 1.)» mmnt. or as.»
Wmummmmu solvent. (3, 6). Bothetthesemtlmm
Wmmtimudmmmwummm.

mwudmmawphmw,wmmm
unm,mamsiummmmuuum.dm
"Um his, unclean; otha‘ IWm'uo new. 22¢me 01'
Momuamdupnmmummcmumm
mmmmaumumnmwuuu‘
Wax mum ofnaphthaléne a ”main ma.

murmmawnwcrwu¢m¢ummmmu
umhwlyhidh «dint-in Waistmnmum"
m,dtbnmotuo-pmhduuid.

{LIPREBKMAL

 

mwngmnwmna mmmflndatmimtionorminio acid,
uuwtmmumwmuwbmmwu. Ban-'0
mwmaz.ouz.ommmcummm
«mwunqmmnumn1uofcumm. The
ammwnnomn.ammmmuum
unumgaywwommmmmmumm. n.
maummmnm°cmmhmuum. mum
mummqnmmuammmuumtmumdu
.mn.mnukmmdumm. Alanindiquatof
mmmummummfiummumm.
mmmmmtannu-memom. Mm
mau.mm.munumumuuamm
mummmuo-pnmum.

cwmmwmmnmvww
summing-m. “append,“dmhhi-dubnnyum
wwnuummuwmuma.mhumm
mummmmmmmmummm. n-
Mon-,W,wadhmuu~omhm¢
m. MmWhMaflanW-am
uwmmummmwmmummu
m. thqunMMmW-fl
tmmmmnwmu.

1 ”ran: any hint film-nuns. m M in .11 flank solution:
mod. ‘rln mucus blank nmmommg-aat, inmost
“.mummreo-Sfiidmwnmmmm
tun-mu.

mo- (7) mm mm a gnaw” «st to: an identification
dWWumlmmanummW
naumwmuw. BuWihttth-W
Mnmwdmmdwwwm,miy
adeu-uufi.

anamwpumwmumtmnm
MWmdmm,u-pummwammw
wmummmmmam Comm
WW,mem,mfiommuqumum
“60de. matumnagm,wmmpuondmtu
W,Mdmmnmwbmuidnfim
magnummtanmmummm. Yin-mm
WWMnmwmmnMfiImuudoW
mw,amoummommnaoimmmw
Matthieu. mtwtmmqmummwmmm
(mumnm‘mmmwnmmmrm
umuwmmmmnmomum-aw
muuuwmwmmummum
tum.

memunmmwmmmmmw
mwummgem m'mcqmly dim-attack
”mum-amass“. '

mamwmmuumaum
Maw,-M~mumuwm
umumwum. mmm
”WEWWyM,an¢h
muwmwmm,ummwpn
Wyumnmm.

muupunmmmuumwm
mw,numt~mumhm
maumwunwu-‘wmn
WWW. ramming-Maw
umummmnmmmumumm
distal-“MW.

 

mwmwbmdm¢wm,u
unmmummmwuu
min-m. “.mmmmgmmn
whoa-rm.

mmmu-mwm.wummm
amusmmuummumum.
Mint-Wm.

will
8WICCIWMR

 

 

 

u.m¢ww.mcmt5&0mmmm

Hashim-y.
ammummmbyamummuomu

mane-.mnmmmmimummunmm

TABLE I!
W U SEWW mm

 

mm typo W Ending

 

cum arms ‘ 11.5
Coming 5mm u:
cw; ih‘lfih 15.1
MAME»? 13:?
Calling #008 1.6.0

 

mmmmfimsmammmmuwmmuw
“ammunwu. mama tum. fhlmnad
Imbmmmcmwnw.mmwmum
“mimhtmequI-MW
an. Communtbummumutmammtw i135“.
hmmuuwmm,mummmwmwm
Wumumqummm
infill-Moon.

  

“mWMmMMth-uflm
ummummmupaflmmuwammmatpfl

coin-11mm mmzydnmm solution, mamtm
WWIuonoto-ghthano uidvithmeinal. nun-2mm
fin mam hphthdio acid «mm M an essentially constant
mmwmwmmmwumawmmmnsfia
mwmnmmm'mwnummw.
bwhlmmom-Incflmtdflanmmwwm
WMMfimpflcmmluMm-mumumpan
matuthansofi

  

wwnmmmummwmmn
max-11mm rmmwmmm,m,
mwumnmmmfisuu
manna-Wan. omw,mmhm.mu
mmmzynwmm. than”,
itadaciddtomkinmiutimMmlJlnm-twinndiu
W. Inthunmmmmnmomumwumh
annals, mtmmnmum.ummam 0,205.0");
“Mummtmwwummnwuam.

 

mruaummmwmmmm
mumummmamwm. Wu
mmnmmwuimmmnwmwmm
Wmottrumluhlimdrwrciml. W,hnmuonof
hmfihobtflmdmingtnwmiudmmimimd W

INTENSITY

FLUORESCENCE

INTENSITY

FLUORESCENCE

 

I
I

 

I
I

I
I

I0— _

 

o I III

 

 

 

 

 

 

 

 

O 2 4 e 8 IO I2 I4 Lsu 2M 2.5M
pH OF FLUORESCEIN SOLUTION

GRAPH I. EFFECT OF pH ON SAMPLE FLUORESCENCE

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

I III I
o I” I 1111 M

pH 0F BLANK SOLUTION

GRAPH 2. EFFECT OF pH ON BLANK FLUORESCENCE

10

Wyn-0mm, no “mince“ difl'm an observed guitar
mmimaplmd. mcupluundinthumdymlfinl.
mm.“ no-phthnlio wuhuoawnuining 1.0 mg. per E1.

1mm

— ‘- ‘fimwo .- u — *- "M-wi-u-‘W- mm. .

    

 

 

 

mmmrmowmmmpmuobw,u
mmmmmnumm-wmammuga «visual
ha“n.inudhcmuflh,lbm,mbbovitmm
mudanmihintyutth-thod.

mmamcmmmmrmmummm
muWMqauu-nmmummmmm
kammemequ-g threw,
mbymmmmmwuunmmdm
m.

6, ‘ .. Effect 01: Va.- ‘* ~22 . as o! S A} ._ 5...“! £133 Irztena «-
MIMI-mom

mammmmammmmumu
unarmmm-mauphmdummmnm
mm.mwmmdum,mm¢emw.
sumuwnnflmmnwmnpmmmau
mmwm. Wfl.mumdmmbm widmluticn

Ming.p¢n1.mmdauupho.

 

 

 

131... I?
mm mm: W W ACID

'01” 3330‘ 0.01 .1. 0.05 .1. 0.19 .1. 0.15 .1. 0.20 ‘g
m Bonding ‘

Sup); 1 113.2 113.5 13.3 11.8 8.28.
W Reading

I.“ I! 12.0 15.0 ”00 nos 9.“
u. . T “ ‘ ‘ ”L :1”

 

mmnmnmumamomu.dm
WW and, ”luau-lyinginmmgn 0.01-0.0511.nd0.05-
unmanmaumummapmmm
”has.

TABLE'

"Wm -fl~v~ macs-nu..." Mw‘u'm U-W~-'an- ,

 

701:.- nfio. 0.02 ml. 0.03 In. 0.01; :1. 0.56 :1. 0.07 :1.

mm ROW
w flood“

3‘91. 11 1B5 ”4.5 115.5 114.0 1b.!)

 

The results; show that an essentially comtmt, maxim flumscema
13 Obtained with all whats of mifxn'ic acid in the range 0.32-0.05 m1.
Suntan, 0.3:. :1. a: concentrated sulfuric acid in. Islam 83 the
0pm- vflnn, an. it Want: the middle or thin range.

I T-"o Raw

 

In tho (mum at tho optimum than a! "sedan the oven tatgzeraum
Inn-Wm: «125°C mo.cmd.ammmm acid
inland. nmzmuwmcmwguoagm: mama.
In. againuod tar tho Maple martian.

TxflLfi VI
mm RWIQ mm

 

-u - Mat-I’M. “a... ‘W- “a. I”. wwuw “W “‘0‘: NIP-M” .Hoo-- ‘ '0de ‘-'-r‘ 1’4

Bonuurim 122'. 1hrJ521n. 112153013111. 1!!.I45n1n.2h%.
W finding

M1. I 5.65 1,300 than ”5.2 1,400
w landing

0-91. 11 1.50 limo 1.1.8 11w 1h.o
m “” .__ :1: .___ ““51 M ‘ 3:2": “I 1“ I A”

 

 

MWMMOitummthnmnthi-mm
Mummummnm. Km,nnmuanu7m,
MWUHMumOpun-umumwmny
“Ml-awn. I
0-W

humdmnmwnmmfimn
mmmnflmmmmmamm

13

dnfiwmua,cuphu ammmmtmmmmwm
Macaw. Emanwnmcinimmwuw
MMCWNMuninuflwmth-mnwmo
”Wham.

2mm
mmmrm

uh - no- - nu- .. . d _ ‘4‘...“ *‘1-“Wmlrw- as“- -1 “IV" "Il..-a-- Mun—t vm-H‘Wumwmmm o“. -—.~-W--*wn=z—-m- vb“

   

 

Sapl- I 6.16 11.1 1%.! 0.28 11s.! 0.59
8‘1. II 1.15 13.5 115.5 ‘ 0.2.1 313.0 0.58

 

ammmmumumummhum-W,
mmyxmwmnwWWMa
I'm I‘v-mmmnmwwummnm
mvtdhflthIOJ-OJ-Im. hum-11mm;-
www.mmmmumuwgm
mug-manwcwm.

MW
MermromtiOhth-MUnppmm,"

W. Iimtoulth‘flumitiudo-mhnuwhuun
Sonata-compound, itmdtnod‘hmw

III

"lulu. mumamuunmummom
mamw,umm,uMa-mu
mun-many. CMEMQIIIIWU
“Okinawan-1h“. tumumlhnmpmd
wumudwnmnm. runway-cum
wnwumo.fiu.nioom. Mm,thuppur
“mmiumum-unyu,dwmmu
ammomm,mmmmuunuuumur
W.
rumwummmunm,mmumm
mmmmdmummm. roan-OME-
Wwvmmurmwmmw
ammm,mmmmum~mm. rho-i:
mumm-mdmm,ummmw. Each
MQNW,W,WuI-mmnm

mum—pun.
mun

CWO! (U mm can MA

W

i o d 1 6 A
50 0.800 0.850 0.770 0.850 0.825 0.770 0.821“
75 1.10 1.18 1.15 1.22 1.12 1.13 1.15
100 1.58 1 .55 1 .55 1 .60 1 .50 1 .50 1.55
100 3.05 2 .95 3 .05 3 .05 2 .90 3 .00 3.00
m 050 hose 1‘08) I158 huh; 1‘50 “.52
w 5080 5.79 5.90 60m 6000 $095 50,1
SW 7 .hS 7 .h0 7 .II0 7 .50 7 .5 7 .20 7 .38
0m 11.0 11.2 11.5 11.5 11.5 11.2 11.3
m “.5 “‘00 11"; “.0 MOS “.2 ”‘03
1000 18 .2 28 .2 27 .5 27 .S 28 .S 28 .0 28 .0
3000 no.5 ho.s no.0 ho.o 1.2.0 Ia..5 h0.8
woo 50.5 h? .3 53 .0 S2 .2 55 .5 53 .0 52.3

 

.:

3

3

go

3

3
o
2:
o
o

15

tramwalm(mlndh)itmnndihhm
Mammn-nwcmumnwmiwv
uwmwuqmmm. immuno-
nofithdbflmumu-plmwtmnmflw.
m,mmunmmmmumrwtooougooom
"Mmuwomummmrwmtmnm
"In. '

mmwumomumWw-ammwmm
MmdemumWAp-wmm
mummummsmOnmm-mmnm.
“Mmew,mumwm-m
W'Mflmhhum.dwwwd.uw
“ummus-u.unhunuwwu. tho-MIL
Mmmummmmmmm
annual-u mm,uu1mmn,u 83.7.100,k19, 500, £31
umwwammuuuwn. Oufl.mmdthou
Mummiamuwumumm
mm mmuwm,mwwm
mmvmummmmmm,mm
murmuumm-mmu». indium-£311.
m.

uuumwam-Wmmtmmm
1w,umdmrphmwmmmmd
Mime-utmost“. torso-Lbdnuoudm
wwmwmmpm,nmqu-amm.

INTENSITY

FLUORESCENCE

0|

.5

16

 

 

 

 

III! II

 

I

 

I00 200 300 400
MICROCRAMS O-PHTHALIC ACID REACTED

GRAPH 3. CALIBRATION CURVE (I)

500

 

INTENSITY

FLUORESCENCE

l7

 

 

 

I

IIILII I

 

 

 

 

500

I600

2000 3000 4000 5000
MICROCRAMS O-PHTHALIC ACID NEACTEO

GRAPH 4. CALIBRATION CURVE (2)

 

TABLE :1
WM?!“ C! CW2“ CW3
rim. Iii-rap.- rpm mmtgphm {from

i
1.” $1.8 83.7 4.3
105s Mgo 100.0 0.0
6.22 1121.0 “9.0 «3.6
7.“ 515.0 500.0 0.0
“I: 915.0 WOO do;

mmmmdumm-umnmm. run-p1»
mummdwmuhtimuwWOb
mumps-mums“. Aawmdmifimfi:
wuuwnummummmmmm
WW.

TABLE I
hummus: G mm W ACID wuss

 
 

 
 

fl' 5‘“

HM m In. «’50 E1. 9c 50 x1. Percent
1.10 139 6.95 1.00 0.1
J.” 22'! 11A 11.0 3.6
8.87 585 29.3 29.0 1.0

15A 1093 SM 515.0 0.9

 

 

mmmtmummqum-pmm
“.muuxmmmuummmmmnmm

1?

acid phflnlato cautions we checked against tho causation curve.

11..,-,_,,_,;,~->.~ .. :53; 34,913 ,s. _' . .. . Cvg;

twmmmmmmamnrunmmmum
magma-u map emanation dthnmmltoyiold nmmont
w. harm-wmummuuo-phmamuu
Womuumwmumnmm.mtmm
“WmmmhthomdtMnum. m.
Wuamammmmmvuamummgp
~.~mm..mmmupnbmfioumnmhm
qua-titan thtwmnhmnumm int-lay. ndobyit.
nun-mu...

In m- ania-um - ablation ”tn-ma with 10033118110110 and my.
mmammwmsoomwuo-pmmuuwu.
no“. Ml.0d.mmmmtbmmmoottho
WWMdflOIiIO-pln,muudhdotmimm
Whmcmunm. mmmumm
hut-m,mtmuhh9&mromuwll. thinénicrw-
Wmmwmmmmamumi,mum
wwwmmfiaflmmnwwoanpm.

ammmma-naphtmummwxm.uum
damn, n u:- mu-m Wofnafihthalem a mum. and, In
.ttuptmmuappummmmmfimutmucomu.

mwmmmwmpmm «not cum-owed; ham,
thwaudm-nmhthqdm-mewmaumulm
“kWh-flightmminmintwtw.

TABLE 1'1
mac: (3' mums CORTEWS

 

 

 

 

Satan-Om With Suture“; Isl—Eh that“; with 13330:

 

Contaminant lemon Acid a—naphthoquinom Acid and
a: r d-napMW
Him-09'.-
o-phthllia acid
taken 1000 1000 1000
11mm ,
”hm-lie acid
but 990 . 915 950
PM Err. 13 2.51 5;
A _w_ w M

 

 

 

 

elm-paw...“

murutmpmummumwwuum
madma-naphW.MumdSSII-M rho
m,m,uamunqummhmma
a-naphmmuoa-tm.
uwWom-mmmwuummmu,
mawnmmumm. Mm,moflntdtho
Namath-Muhammad. nun-mama»
Wtdhouflatdyitmumwont. TMWW‘MW
mwmmmumumumw,mmun
«mmmwmmnmmm

n. W
Tho ransom remind to: «flying out this determination m;
www.mumammmummamm

«Mlflmtwmxido.

3

Anamoolnfiondo-qmmuunhmuhmd. imam,
fllwmuhhd-W,dhmnamwaaphhu
wmam,mawmummwm'
“Manna-nary.

Llfifl.mwtdihmumpoluumbp1pothdmc
smmmoamamwmmmwuwnm
twat-11.9115“. tMSthIgn.orcmudhpIn-o
mumummwmumm
nth-ml. tho-plummnplmdhnm,uammnod
www.mamuuumm. nun
wummm,mmmmmmam
«Mucous-mm. mummumthl
mmmwuumuwumwmmmu.
woman-«mam. nuufimcwowm
"datum,”wmwwuwum
mmm.,m,wmunumum¢m
wwmmwmm. manages-10.1.»
uuwmum-zon.mmummum«ua
balm-LN“. mumm.ummumu
coda-W.

mmmwm.mwamm.mww
mwmwummmummm
«Mamma. cum.ummwummi
adamanm-mmmmmmamuud
Wmmmsomwmmw. nus-sauna

mmwawummmmmmmtyammu
W,ny,mmtm1fimm:uphnw
mmv. For owl-,innlsomroynmru "madam
mmmmmmuwumom.umzxmhymm,
hMVdmmnmmmeMawmm
mmmmwummnmmwmnuun
ammummmmumozmmnmmmo
m. wwmumummamcma than
mum-.maummnwummpu anal-mm wan-1w
wwwmncuuummmztmm
mmmmnweamamummwum
alumna“. tmrm,1$mm1mdmmahtmml,
lMdW,mmmfidflth-mmormmv
ammawmuumwummmmm.
mmmm,mmmmmmm,uw
mamamwtyum. mmmumuun
ammuummhammmnmmvdm
Malnuuhnadm‘mummung. Rumm-
W,mummm1hm-y,uwtummm
www,aummmmumum.uoo-
mm. Ammm,¢wmmmmtam
rug-bdngawud, in atrium rubl- far mic pun-poet.
magnum-cummtydtmulnuamm-om,
mmvawmwnmdmgutmwm
deWWfifim-GWM.

Althoughmcorroouonh mpued tarbhnkmorcm,uimu
ummwumwumuwmmnoguogmu,
ltuamomtopmpmabim,uumm1,twnacbmd
unlaw-

nu calibration curve 1.- pnpmd by reacting o-pbthalic «:13 m.
m,¢mmaum,ummabonmaemmngmu
W0 nun-11.1”. Aplototnwmc immune: eta-dime
unimmudwhwduwumtadumuuflmmua
Wm.

tummmmumdammmumcmgfifiw
“WWIflmmthMEMSIHWMuIRPv
15.91“ mm “ah abut-ho, mac-1.11, tho 36$ alum-naming)
”in.

EUEULEX

A 5M3 nether! ha been museum-:- {m- m: detmbmtinn at
o-phtmna mid. Comma-luau a! o-phthslilo acid ranging from 5-3 to 5000
Inform pt! 31., can be detmimd in th- W. of beanie, ism
phallic and tertphthnlic acid: with an expected u’udnn mar of approx}.-
ntdy M. In tin pream- ota-naphtlnquimm I slightly large:- error,
Natal: 55, in to be enacted due in color {nation resulting in
m1 abut-puns! of fluorescent mission. The time remix-mm of tha
flatbed :- appromately two home .

mmwaa (SHED

25

1. £01.51, 1., .Wfl £3113”.- by 3130‘ 1W, 9. 29?, 3mm
Wm; Co. 1.13., an lurk, (1939).

2. m, c. 5., mm ramming, Vol. 23, no. 1;, pp, 2.2.3.251. (193.12).
3. nanny, 1.. 3., Anal. mm. 1.2, sea-m (191.7); g, 831-73 (1929).

h. antenna”. 1., 319813,“. 1., md‘Blaisdall, A. c., Ini.1’ngf?1xm.,
Am. n.3, 320-21. (193.5).

5.0.5.11, ..I. mm, c. I. ml. Eng.)Chu.,Am1.Ed.LRGh-5
(1931); 5: aid-So (1933) 3m 3, 570.5 (193:3) .

6. had, I. n. and m, 2., man. Chm. 35815-21 (1913).
Y. Dodson, 639., It". “on. Magnit- ”mum, Hi: We. 68. 3-5 (17159).

8. MW! Bulletin Ember 305 Mating mm for the fiwtovalt
mm mm mm 5614.

26

W
WOW,MNmnm-wmumtmm
mmmgnwmmmmflmmw
h,amm,omtmmdnmtmmu¢om
“Mmuomumufihuumvwm. “option-1
«Mpnuummuwomumummm
“dnumuummmm. fiflMpl’hlflflfll,“
”manuumcmmammnwmmm
tun-mm.
mmmummmManW
mummmnmmhmtwmummm. Aphou-
mumwmnmumuumh.
mmm,ummuugml.uamgdumm
lawman. tumutnmumammow
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33

THE FLUCEQIETRIC DEM-{INATION 0? CW ACID
INCLUDING THE ASSEMBLI (F A FIEORCMETEE

B
y i .

Glen A . Thomas

“ABSTRACT

Submitted to the School or Grad-who Studie- ai‘ Michigan
State College of Agriculture and Applied Science
in partial fulfillment of the NW
for the deg-en at
mar-53 W son-ms
Department of Chemistry

Year 1951;

Approved WW _
U

Glen A . Thomas

THESIS mom-gr

A fluoronotric method has been developed for the determiner-ion of
o-phthelio ecid either slone or contaminated with various other carboxylic
eoids . The method involves tho couiemetion or o-phthelio ecid with
resorcinol to tors fluoresoein. The condensation is effected through use
of concentrated sulfuric said end e reaction temperature of 125°C.

A solution or the sodium salt of the fluoresoein {cried in this manner ,
yields an intense goon fluorescence on excitetion with ultra-violet light
and the intensity of the fluorescence servee es s qusntitetive measure of
the quantity of o-phthelic ecid re acted.

Concentrations of o-phthalio soid renging Ira 50 to 5000 micrograms
per 1-1., can be determined in the presence of beneoic , isophthslic sud
terephthllio aid. with en expected nun-m error or spproxlnately M. In
the presence of o! ~naphthoquinonc e slightly larger error, approxinetely
5% is to be emeotod, The time requirement of the method is approxinetely
two honre. ‘ I

A direct reading fluorometcr us essembled , employing s Photovolt
Beetronio Photonoter nodal 501-14, for use in this work. The appendix of
the thesis, {run which this abstract is drain, contains instructions end
diegrems describing the usenbly of such on inntment.

 

AN STATE UNIVERSITY LIBRAR. E5

II I III III III IIII

93 031773