Low copy number DNA deposited on an improvised explosive device (IED) is typically subjected to and degraded by the high temperatures during deflagration, creating a situation where it is difficult to identify the assembler. Often, when IED fragments are sent for analysis, they are analyzed both for explosive residue and fingerprints, leading to the potential loss of remaining DNA. This research examined cyanoacrylate (CA) fuming of pipe bomb fragments immediately after deflagration and its effects on the quantity and quality of DNA collected from the IED. This allows for determination of a proper order of processing for IED fragments. Twenty-four volunteers were asked to mock-assemble pairs of pipe bombs, one of which was CA fumed after deflagration and one that was not. DNA was quantified, amplified using an AmpFlSTR® MinifilerTM PCR Amplification Kit, and consensus profiles were developed. Comparisons indicated that CA fuming did not hinder DNA recovery, but due to high variation it could not be determined if it resulted in greater DNA recovery. Additionally, fuming did not alter the quality of the amplification product or consensus profiles. The decision as to the order of processing of the pipe bomb fragments, including whether or not to fume them, should be made as soon as possible when they arrive at the laboratory.
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