RELATIONSHIP OF INTERNAL TJMPERATURE REACHED AND MAINTAINED BY VEGETABLES D73 NG BLANCHING TO QUALITY RETENTION DURING FROZEN STORAGE by Jean Carstensen h A THESIS (Submitted to the School of Graduate Studies of Hichigan State College of Agriculture and Applied Science In partial fulfillment of the requirements for the degree of Master of Science Department of Foods and Nutrition School of Home Economics 1950 ACKNO'. .‘LEDGXL'N T The writer would like to express her sincere appreciation for the expert and sympathetic guidance of Dr. Pauline Paul. She would also like to express her gratitude for the efforts of the judging panel, Olive Batcher, Haura Bean, Katherine Gaffner, Ruth Ingalls and Anna Nae Redder. Without their cooperation this study would have been impossible. f’ftri.? a2=)_ i 27,, TABLE OF CONTENTS Introduction IO...O.I00......5....OOOIOCOOOOICOIOOIIDO RGViev, of Literature 0.0.0.0090390000.00....JIOOCOICOO Relation of blanching to enzymes and keeping qualities of frozen vegetables................... Enzyme activity. Effect of heat. Index of adequate blanching. Tune-temperature requirements. Variation with length of storage. Nature of enzymes Early work. Recent work. Enzyme characteristics of different parts of the plant. Loss of Ascorbic Acid with blanching............. Effect of freezing. Extent of loss during blanching. Blanching medium................................. Effect of blanching on texture................... Procedure............................................. History of vegetables............................ Preparation for freezer storage.................. Blanching. Hater. Stemn. Cooling and packaging. Freezing and storage. Temperature records. Chemical and physical tests...................... Ascorbic acid. Total solids. Enzyme test. Texture. OI‘ganCleptiC testOOIOOOOOI'OOIIIQUOICOOOOOOOOOII. 13 15 16 18 18 Discussion of results................................ 23 Temperature reached during blanching............ 23 Increase with time. Variation with size. Variation in temperature of medium. Difference in vegetables. Palatability scores............................. 28 Broccoli. General appearance. Color. Flavor. Odor. Texture.‘ General conclusions. Cauliflower. General appearance. Color. Flavor. Odor. Texture. General conclusions. Analysis of variance of the scores.............. l0 Broccoli. Cauliflower. Objective tests................................. Hi Ascorbic acid determinations. Broccoli. Cauliflower. Total solids. Enzyme test. Penetrometcr readings of texture. Cauliflower. Broccoli. Smary and ConCluSions‘ooo‘oon.coco-coonooo-ooocoulloo 56 Graph Table NO‘UL-F‘UJN FIGURES AND TABLES Average Internal Temperature Reached by Broccoli wring BlanchingIOIIOOOIIOOOOOOIIIOOOIODICOOOIOOI Average Internal Temperature Reached by cauliflower Du-ring BlanChingo o o O o o o o o o o o a o o o o o o o c 27 Mean Scores for Treatments - Broccoli............ 33 Mean Scores for Treatments - Cauliflower......... 39 Analysis of Variance - F Value................... Q3 Ascorbic Acid Content - Mg/lOO Gram (Wet Basis).. A6 Percent of Total Solids...-...................... i8 Peroxidase Blanching Test........................ 51 Comparison of Penetrometer Readings and Texture Scores-Broccoli-0.0.0.0000.0DD-OOIIOOOIOOOCII... SLI- Comparison of Penetrometer Readings and Texture Scores - Cauliflower............................. 5 INTRODUCTION Blanching vegetables prior to freezing refers to . heating the individual pieces with boiling water or live steam for a specified period of time. It is now established as an essential practice in the preparation of vegetables for preservation by freezing. Evidence has indicated that the following benefits are derived from blanching: 1.) partial or complete inactivation of the enzymes, which of themselves or in conjunction with air, cause poor texture, aroma and flavor during freezing storage, 2.) wilting of the product so it can be more easily packed, 3.) expulsion of gases that may promote oxidation, L.) brightening or setting the green, yellow or red colors in vegetables, 5.) washing and practically complete sterilization of the fresh product and 6.) dissolving some of the undesirable flavors in certain vegetables. The heating that occurs in blanching, however, often causes profound modification in texture and reduction in the amount of aroma, flavor and soluble nutrients in vegetables. Therefore, there haVe been many studies conducted in an attempt to develop blanching procedures which would 1 insure the advantages derived through heating, but which would result in a minimum of the undesirable changes. Attempts have been made to see what variations in blanching practices would result in products of the highest quality. The main objective of this series of experiments was to determine the effect of the internal temperature reached and maintained by broccoli and cauliflower during the blanching process on the quality at Various intervals during subsequent frozen storage. Different mediums and time intervals for blanching were used to produce variation in the internal temperature. REVIEH OF LITERATURE Although all investigators agree that blanching is necessary in the preparation of vegetables for freezing, there is much difference of oninion concerning the best temperature, period of exposure and medium to be used in blanching. Probably most of this difference of opinion can be traced to the different objectives of the respective investigators. Relation of Blanching to Enzymes and Keeping Quality of Frozen Vegetables Enzyme Activity Effect 23 Eggt. In the early investigations in the field, most of the attention was paid to the relationship of heating and enzymatic inactivation to keeping quality of vegetables during frozen storage. As early as 1929, Kohman (a?) pointed out that cooking vegetables enough for serving stopped respiratory processes and preserved the flavor of the vegetable in freezing storage. Joslyn (hO) was also among the first to report that Certain vegetables heated through blanching and packed in brine were successfully preserved by freezing, but similar samples not scalded deteriorated in appearance and flavor. At this time, he 3 attributed this deterioration to the action of certain ripening enzymes, which low temperatures retarded but did not entirely inhibit. Woodroof (87) observed that although freezing greatly reduced most biological action, it did not entirely prevent respiration processes that resulted in loss of fresh flavor, aroma and color. At this time, he predicted the possibilities of preliminary scalding or blanching of the raw product as a solution to the problem of enzymatic inactivation. Tressler (77) stated that vegetables blanched and quick-frozen were found to retain their fresh flavor even when stored for long periods of time. lgggg 2f adeguate blanching. Following these early reports, there were numerous studies conducted to determine what enzyme could be used as an index of adequate enzyme inactivation. Diehl (28) indicated that there was a definite correlation between heat penetration and catalase activity. He suggested that scalding sufficiently to destroy catalase would prevent enzyme deterioration. Later, Tressler et a1. (81) agreed with these findings. Smart and Brunstetter (72, 73) found that when scalding resulted in the practically complete destruction of catalase, satisfactory packs of lima beans, spinach and kale could be expected. However, Alrighi, et a1. (3) stated that it was necessary to heat at a higher temperature than that required L attributed this deterioration to the action of certain ripening enzymes, which low temperatures retarded but did not entirely inhibit. Woodroof (87) observed that although freezing greatly reduced most biological action, it did not entirely prevent respiration processes that resulted in loss of fresh flavor, aroma and color. At this time, he predicted the possibilities of preliminary scalding or blanching of the raw product as a solution to the problem of enzymatic inactivation. Tressler (77) stated that vegetables blanched and quick-frozen were found to retain their fresh flavor even when stored for long periods of time. gnggg g: adequate blanching. Following these early reports, there were numerous studies conducted to determine what enzyme could be used as an index of adequate enzyme inactivation. Diehl (28) indicated that there was a definite correlation between heat penetration and catalase activity. He suggested that scalding sufficiently to destroy catalase would prevent enzyme deterioration. Later, Tressler et a1. (81) agreed with these findings. Smart and Brunstetter (72, 73) found that when scalding resulted in the practically complete destruction of catalase, satisfactory packs of lima beans, spinach and kale could be expected. However, Alrighi, et al. (3) stated that it was necessary to heat at a higher temperature than that required a to inactivate the catalase to insure a product of good quality. Joslyn (#2) concluded that in general, if the scalding treatment was not sufficient to inactivate the catalase and peroxidase, frozen vegetables would develop off-flavor. Conversely, processed samples off in flavor, contained active enzymes. 0f the two, peroxidase more closely paralleled the off-flavor formation than did catalase. Bedford and Joslyn (7) found that in frozen green beans the catalase activity and acetaldehyde content decreased as the quality of the product improved, but they were not a reliable index of flavor retention. The ascorbic acid oxidase activity closely paralleled that of catalase. They maintained that peroxidase activity would serve as a better index of quality than would catalase activity. Joslyn and Harsh (Ah) determined the correlation between catalase inactivation and flavor retention to discover whether complete inactivation of peroxidase and ascorbase and phenolase was necessary. They observed that even though vegetables gave a positive catalase and peroxidase test, they retained flavor and texture for short storage periods. It was difficult to correlate the qualitative enzyme tests with keeping quality over short periods of time. However, quantitative data on enzyme activity indicated that complete inactivation of both peroxidase and catalase was obtained in samples judged to be best in flavor for certain vegetables. : I Kertesz, Dearborn and Mack (ab) concluded that the most desirable duration of time for blanching peas was established by a determination of the catalase activity in the vegetable. Heating required to inactivate the catalase also inactivated the ascorbic acid oxidase. In l9h0, Diehl (21) studied the quantitative relation between the activity of phosphotase enzyme and the retention of quality of asparagus during freezing. In very recent years, there have been further studies to substantiate the findings of these earlier investigators. Masure selected peroxidase as his index of adequate blanchr ing (56). Cruess and Smith (18) in an examination of frozen fruits and Vegetables on retail markets in central California used peroxidase and catalase enzyme tests to check on adequacy of blanching. The authors believed that blanching should be long enough to inactivate the peroxidase enzymes. In their estimation, it was not enough to merely inactivate catalase by blanching. Campbell (12) recommended catalase test for peas. In his experience, peas exhibiting a negative test for catalase activity never exhibited abnormal color when properly stored. In the latest available report, Fitzgerald (33) stated that enzyme catalase, which he considered the chief offender in poor quality in storage, could be inactivated at a lower temperature than enzymes such as peroxidase. However, in the case of green beans and asparagus, inactivation of the latter enzyme was exceptionally important. 6 Time—Temperature requirements. Following and concurrent with.the work determining what enzyme could be used as an indication of adequate blanching, there were many studies on the time and temperature of blanching needed to inactivate these enzymes and maintain the quality of frozen vegetables. The earlier investigators recommended longer periods of blanching than are now being used, either because of their anxiety to completely inhibit all enzyme activity in the vegetable or for some other objective. Recently, more emphasis has been placed on determination of the extent of inactivation required. Joslyn (MB) found that blanching peas, string beans and asparagus for sixty seconds in steam made possible flavor retention during freezing storage for more than one year. Diehl (20) stated that at low blanching temperatures, (200°, 180° and 160° F. ) even for comparatively long periods of time, the catalase in peas was not inactivated, with subsequent deterioration in color and flavor, even at 0° F. storage. However, negative tests were consistently obtained with exposure as short as thirty seconds at boiling temper- ature. Smart and Brunstetter (72) found that scalding for two minutes at 212° F. or twelve minutes at 190° F. would inactivate the catalase enzyme of lima beans. Joslyn and Harsh (uh) said that there was considerable difference in flavor and odor in ranges of blanching 7 tenmperature from 1680—1710, from 1760-18;0 and from 1900— 2120 F. Samples of spinach and peas blanched in the lowest range were of poor color, odor and flavor after two year's storage. Those blanched at temperatures over 188° F. were satisfactory in color and flavor. Later work with green beans, (Bedford and Joslyn, 7) Substantiated these findings. Green beans blanched below 80° C. developed undesirable odors, flavors and color during storage. Between 850-900 C., there was best retention of quality. Above 95° 0. most of the samples lost their string bean flavor. Arighi, et a1., (3) obtained a satisfactory product by scalding peas for two minutes at 800-900 C. This was at a higher temperature than that required to inactivate catalase. Peas blanched for various periods of time at lower temper- atures were all found to be undesirable in color, odor and flavor. Investigators have not overlooked the objectionable changes caused by overblanching. (3, 8, l9, h3, 72, 86, 88) Overblanching caused cooked odors and flavors and mushy textura.not usually associated with frozen vegetables. These increased as the temperatures increased. There seems to be a critical temperature range below which the formation of off-flavor is not entirely inhibited and the color is not "set". Above that temperature, there is a loss of fresh flavor, formation of a cooked flavor, and softening of texture. The temperature apparently is different 8 \ IA--~ c. ““2... . for' different vegetables, increasing with the intended storage periods. Variation EEEB length 2: storage. Variation of the enzyme inactivation and the length of storage was mentioned by Joslyn (42). He stated that complete inactivation of the peroxidase was not necessary for storage of three to seven months at 0° F., but most of the initial activity must be destroyed. Masure (56) also mentioned the possibility of blanching in relation to length of time the product was to be held in freezer storage. The results of Woodroof's work (88) showed that the quality of vegetables considered to be underblanched could be maintained for a short period - three months in this case. After this, there was a rather rapid loss of quality, and at six and nine months, the vegetables that were adequately blanched were higher in quality. (Nature 22 Enzymes Most of the blanching procedures were introduced before there was much information available as to the nature of the enzymes involved, the substrates acted on, and the products formed. There were some early studies on enzymes themselves, but it has only been in comparatively recent times that extensive investigations have been conducted. @2311 £233. Typical of the early work was that of Kohman (A?) Who believed that changes in flavor during freezing were brought about by a process of anaerobic respir- 9 atiLui and more directly by resoiratory enzymes. They based this on the fact that during freezing, macerated vegetable tissues act as a barrier to the penetration of oxygen to the sound tissues underneath, which then undergo anaerobic respiration. This view was not entirely in agreement with' Tressler (77), who stated that most enzymatic changes in foods Were both oxidative and hydrolytic in nature. Joslyn and Marsh (A3) agreed with both of these invest- igators. They said that the chemical changes in flavor and color were due to oxidation, largely arising through the oxidizing enzymes, and changes in flavor were due to anaerobic respiration and other causes. In 1938, Joslyn (kl) pointed out that enzymes had been found to be unaffected by exposure to 10West temperatures investigated (liOOO below 0° F.) and many of them were extremely active. He claimed that enzymes must be inhibited by preventing the necessary combination between all constituents of the enzyme-substrate system or by destroying one or more of the components of the enzyme by heat. Lineweaver (52) concluded that a study of the energy of activation of enzyme reaction should make it possible to predict the nature of major changes during storage. Diehl (21) ascertained the stability of proteins present in peas under freezing storage conditions. He found various reactions, some varieties of peas showing protein decomposition and others protein stability under the same storage conditions. He considered the possibility of development of undesirable 10 flavors as being due to changes in the fatty constituents of peas. Recent work. However, not until 1942, did Balls (h) conduct investigations into what actually happens to enzymes during blanching and freezing. He believed that all enzymes are proteins and consequently the problems of destroying enzymes by heat and the effect of heat upon proteins were the same. Heat denaturation and inactivation of the protein occurs when foodstuffs are blanched. However, Balls also pointed out that protein denaturation is not always permanent and the protein may revert on cooling to its native state. This would account for the reactivation of peroxidase discovered by many investigators. (8, 22, 71, 88) Balls (4) and Proctor (68) both remarked that although catalase and peroxidase tests were commonly used for adequacy of blanching tests, these two enzymes were not necessarily those that caused the development of undesirable changes during frozen storage. These are merely indicators and not necessarily the actual damaging enzymes. Balls pointed out that in normal vegetable tissue, the enzyme system is extremely complex and that the undesirable colors, odors and flavors may be due to any disruption and accumulation of intermediate products in the long chain of biological oxidation. He. concluded that total destruction of enzymes is usually not practical nor for ordinary purposes is it necessary. This is in agreement with Diehl, et a1., (22) who, in 1936: said 11 thaj; recovery of enzymatic factors in peas after scalding was 110t industrially important. In l9h3, Phaff and Joslyn (o3) emphasized the necessity for selection of the proper indicator for an enzyme test. Indicators of enzyme activity differ in their specificity, ease of oxidation and other properties. Since the relative peroxidase activity toward indicators varies with vegetables, there must be discriminate application of peroxidase reagents in testing for adequacy of blanching. Joslyn (AB) said it was necessary to select proper indicators for particular vegetables tested. Not only did the relative activity of the peroxidase of a particular vegetable tissue vary with the indicator used, but the apparent ease of thermal inactivation of the enzyme. Only reagents giving a strong positive reaction in unblanched tissues and a negative reaction in tissues blanched sufficiently for flavor retention are suitable. Another comparatively recent investigation is that of Rosoff's and Cruess' (71). It was mainly a study of the peroxidase and ascorbic acid oxidase in cauliflower. They found that purified ascorbic acid oxidase and freshly expressed cauliflower juice catalyzed the oxidation of ascorbic acid by molecular oxygen and by hydrogen peroxide. Some studies have shown that different parts of single vegetables have different enzyme characteristics, Which must 12 'be cxxrne in.mind when making blanching tests. Hergentime (inf) said peroxidase activity of baby lima beans was quite strong in the skin, but weak:in the cotyledon, while the catalase reaction of the cotyledon was strong. Phaff (63) said that peroxidase was the most active in those portions of the tissues where respiratory and other metabolic processes were greatest. Rosoff and Cruess (71) found that peroxidase in cauli- flower was mostly concentrated in vascular layers, in the pidermal layer beneath the skin and in the flower buds. The parenehyma had comparatively little peroxidase activity. Loss of Ascorbic Acid with Blanching There have also been many'studies to determine the loss of nutrients during blanching. The vitamin C content has been most often used as a or terion of nutritive value of vegetables. It is the most easily destroyed and if retained, probably indicates that the other vitamins also are present. Effect 2: freezing Apparently freezing in itself does not adversely affect the ascorbic acid content of vegetables, if the vegetable has been sufficiently blanched. (3, 25, 28, 75, 85, 88) However, if the vegetables have not been previously blanched sufficiently to inactivate the ascorbic acid oxidase, there will be considerable loss of ascorbic acid due to the action of this e zyme. (27, S7, 39, h5, h6, SS, 57, 76) 13 I )l . ‘ ‘ ‘ V. m \I) “It ‘ . u I U rl , . .1 a. .3 Aw Ad .7. a j :M .31. I. .. J” . ..¢ 5, M. Av Ii ~W‘k. «I Luv P4 3 “mi “N .2 .v .5; n... AJ. «.5, a. .. NW v :M .m a 3 a... Extent of loss during blanching ‘Most investigators have found that in the blanching process preparatory to freezing loss of ascorbic acid may occur. Fellers (29) said that the loss may often be as much as 50% in peas and lima beans. Later workers found smaller losses. Tressler, et al., (81) found that loss of vitamin c during blanching in itself resulted in 103 less, and during the whole processing the total loss was approximately 303. These closely approximated the results of Fenton and Tressler (31) who found a 383 loss in peas-due to blanching, chilling and packaging. Batchelder, et al., (6) made an extensive study of the effects of blanching on vitamin C. Although they found that there was no loss of ascorbic acid during scalding and freezing of asparagus, peas retained only 75% of their ascorbic acid and broccoli retained approximately 50$ and 70¢,with water and steam scalding respectively. Todhunter and Robbins (76) showed that the process of scalding peas in boiling water for one minute and cooling in water caused a loss of 30-373 of the ascorbic acid present in the fresh state. Farrell and Fellers (28) found a 33¢ loss in ascorbic acid in fresh green beans due to blanching. Gleim, et a1., (35) studied the effect of blanching on asparagus and Spinach. In general, the spinach lost a greater percentage (63$) than did aSparagus (Ski). With cauliflower, Kaloyereas 1 la U+5) found that blanching in boiling water for 2% minutes resulted in an 18.3% loss of ascorbic acid. As the period of blanching is increased, there is greater loss of ascorbic acid, probably due to the leaching of the vitamins into the blanching medium. (1, A6, 39, 55, 59, 82) Most of the reports in literature have been on the losses occurring during commercial freezing. Phillips and Fenton (6h) indicated that the same trends were witnessed in home freezing. They found that blanching beans in boiling water for two minutes resulted in loss of 13% of the ascorbic acid. The total loss of the vitamin during freezing procedure was about 20%. Retzer,et al., (69) indicated that the loss in the freezing preparation of cauliflower was 18% or 19%, for steam and hot water blanch respectively. Blanching Medium Some investigators have stated that the medium used has no effect on the subsequent quality of the frozen vegetable. They claimed there was no marked difference whether steam or .hot water was used. (19) It is generally agreed that boiling water scalding destroys the natural enzymes present in vegetables more rapidly than does steam blanching. Steam blanching, there- fore, requires a longer period of time than does water scalding. (6, 2);, 38, 69) Some workers have claimed though that in hot water 15 blaxuihing more of the nutrients are leached into the medium than into steam. iI."ner<:i‘.')'.r‘e, steam blanchinr is to be preferred even though it requires a longer period of time. (8, 11, 27, 37, 61, 81, 86, 88) Some workers have found various results for different vegetables. Thus Batchelder, et al., (6) found no signifi- cant differences in palatability and ascorbic acid retention between water-scalded and steam-scalded frozen asparagus. On the other hand, peas that had been water—scalded retained more natural flavor and ascorbic acid during storage than did the steam-scalded samples. Steam scalding of broccoli gave a more palatable product with higher ascorbic acid retention than did water scalding. The work of Retzer, et a1., (69) indicates a possible relationship between the divergent opinions. They found that immediately after blanching, the stemn—blanched cauliflower contained a significantly larger amount of ascorbic acid than that blanched in hot water. However the steam blanched product lost a larger amount of ascorbic acid during the cooling process. Results indicated there was no significant difference between the two methods when cooling was also taken into consideration. Effect of Blanching on Texture Worth noting is a special study conducted at Ohio State University to show if the type of blanching was an important factor in the tenderness of frozen beans. (2) Here they 16 ,0 used a texture-meter, which read the pounds of pressure required to pass through an 30 gram sample of green beans. They found that blanching in steam produced a much more tender product than blanching in boiling water. There was an apparent correlation between readings on the texture- meter and organoleptic scores. Lee (51) has found the tenderometer prominent as an instrument to be used for the determination of the quality of raw peas. Jenkins and Lee (38) used the same instrument and found that it had decided advantages in indicating the tenderness or quality of asparagus. 17 L: - ...»Ar.«..hnwmw.whfflfi a» -bfiffl? - {Hindu . _ I}, I fire PZOCCD"HE ‘V history of Vegetables The broccoli and cauliflower were nurchased from the college food service denartment, After delivery, the un- trimmed heads were packed loosely into crates and held at a O F. until reacv for use. 0 temperature of aufiroxinately h Since the vegetables were received in large quantities, it was necessary to hold some of them for one or two days before they could be used. Broccoli and cauliflower, used as fresh control samoles during the study, were purchased from the local market. In preparation for freezing and cooking, the broccoli was cut into h% - 5% inch lengths and trimmed to remove the attached leaves. The smaller inner leaves were not removed but the leaves extending beyond the head were cut back. The stalks were split and soaked in salt water for approximately one half hour to insure removal of adhering bugs and worms. The cauliflower heads were trimmed, thoroughly washed and separated into flowerlets. " .3. Preparation for Freezer storage Blanching Hater. The samples of vegetables to be blanched in hot water were weighed, placed in a galvanized wire basket and immersed in an enamel kettle containing boiling water. 18 'LhL—r Enough of the vegetable was blanched at one time to fill two packages for frozen storage-—6SO grams of broccoli or 800 grams of cauliflower. Three gallons of boiling water were used for these amounts. The broccoli was blanched for lg. 3 and 5 minute intervals in the hot water; the cauliflower for 2, 4 and 6 minute intervals. The water returned to boiling temperature in the 5 minute blanch period for the broccoli, and in the 4 minute blanch period for the cauliflower. .§E§E§° The samples to be blanched by steam were placed on a wire franc, which was supported on an iron rack approx- imately two inches above the boiling water. Only enough broccoli or cauliflower to fill one package for freezer storage was blanched at one time. The samples of vegetables were distributed as evenly as possible over the wire frame, to facilitate the circulation of steam around eace piece. The broccoli was blanched for 25, 5 and 7 minutes, and the cauliflower for 3, 6 and 8 minutes in steam. Cooling and packaging Immediately after blanching, the vegetables were cooled in cold running tap water. 'After draining, the vegetables were removed from the basket, placed on towels to remove excess water, and packed in pliofilm bags, which were protected by cardboard boxes. These bags were sealed with a rubber band and the packages were placed in a quick-freezing unit. Ereezing and storage After a minimum of twenty-four hours in the freezing unit, the frozen vegetables were transferred to freezer l9 storage, either at the local locker plant or in a SGOTaOe cabinet in the laboratory. The vegetables were held for 1%, 3 and h months in storage, when they were removed for sampling. Temperature records The temperature reached internally by the samples of vegetables during blanching and cooling was determined by means of a potentiometer. Copper-constanten thermocouples were inserted 1) into the blanching or cooling medium, 2) into the base of a com,aratively larger or medium-sized stalk or flowerlet and 3) into a comparatively small stalk or flowerlet. The extremes in size were selected so as to give a representative picture of the range in internal temp- eratures reached by vegetables during blanching. The temp- eratures reached in these respective places were recorded on a revolving drum inside the potentiometer. Later, these charts were removed, and the final temperatures read and recorded. Chemical and Physical Tests Ascorbic Aggg Samples of fresh, fresh cooked, blanched, blanched and stored, and cooked frozen vegetables were tested for ascorbic acid content. In all tests, care was taken to maintain the proportion of stem to flower that would be typical of the vegetables prepared for serving. The method of determination was that of Loeffler and Ponting. (53) 2O a “‘1 v) . ..... MM”, u-AIVWHLWT‘.‘ HWhQIfiAQE ‘ \Z .. :wlr WWI—"lull 1 . Total Solids Total solids were determined using a semi-automatic moisture testerl. These tests were run concurrently with ascorbic acid determinations and on the same samples. Samples were selected to get a representative portion of stems and flowers. These vegetables were finely cut up, weighed and placed in the moisture tester for two hours or until half hour reading checked within 0.05%. This never required more than two and one half hours.- Enzyme Test The qualitative test for peroxidase developed at the Western Regional Laboratory was used. (83) Tests were made on the samples immediately after blanching and at the end of the final storage period. Texture Samples of freshly blanched and cooked frozen vegetables from one replication in each test period were tested for texture using a penetrometer2. Representative stalks and flowerlets were selected for sampling. These samples were Dlaced on a cork on the penetromcter platform, with the cut surface of the vegetable resting on the cork. The texture was measured by the depth, which a cone-shaped piece of metal would penetrate into the uncut side of the stem in two seconds. The total load using the cone was 150 grams, 1. Brabender Moisture Tester 2. Micrometer Adjustment Penetrometer 21 ‘ fie. :31. z (needle bar, h7.§ grams, and cone, 102.5 grams). The two second penetration thee was selected because it would be comparable to the almost instantaneous penetration experien- ced in biting. Three readings on three different stems were recorded. Organoloptic Test The cooked vegetables, at the different testing periods, were judged for palatability by a panel of five judges. The judges were all members of the Foods and Nutrition Department. The test periods were at the beginning of the study and, at the end of one and one half months, three and four months of frozen storage. Three replications were conducted at each testing period. The following palatability factors were judged: 1.) appearance, 2.) color, 3.) flavor, h.) odor and S.) texture. The general conclusion score was the judge's over-all reaction to the vegetable, and not an average of the other factors. The score cards also showed whether the product was accept- able or unacceptable on the basis of palatability. The judges were asked to record any comments they had on any particular product. A sample of the score—card used in the study is shown on page i in the Appendix. 22 a“. ittVr¢1rIPVIuv (1.1mm: LPN). .3.“ *0. *1! .I I DISCUSSIO‘ OF RESULTS L Temperatures Reached During Blanching The average results of the potentiometer for each blanchr ing treatment are presented in Figures 1 and 2 on pages 26 and 27. A complete set of recordings is included in the Appendix on pages 11 and 111. Increase with 31mg. The records of the potentiometer showed that as the blanching time increased, the internal temperature reached by the vegetable also increased. The longer the time was extended, the closer the values for the internal temperature and the final medium temperature coincided. With the longest periods of time used, both in steam and hot water blanching, the temperatures of the vegetable and the medium were at or very near the boiling point of water. The recordings from the potentiometer showed the internal temperature gradually increased from the time the vegetable was immersed in the medium until it was removed or until it had reached 2120 F. Variation Kipp gigg. Within the time intervals, the temperatures reached by the smaller and larger pieces of the same vegetable varied. The smaller pieces approached or reached the temperature of 23 u H. u,. ,. IL! ‘1 ii It .1 J the blanching medium more often and more quickly shah the larger pieces. The difference between the internal temp- eratures of the large and small sizes diminished with increase in blanching time. With longer times, the smaller pieces reached the temnerature of the medium and the temperature rise was stopped; whereas, the larger pieces still had not . completed the climb before they were removed from the medium. Variation in temeerature 23 medium ' T'D‘EJE‘ “he. The recordings also indicated that the medium used caused some variation in internal temperatures reached. The vegetables blanched in steam required a longer time to reach similar internal temperatures than those blanched in hot water. This can be traced to the differences in the range of temperature of the medium, itself. As indicated by the graph, the range in temperature of the water was very slight. Addition of the cold vegetables did cause a slight drop in the temnerature of the water. During the longer blanching periods, the temperature returned to the boiling point. The addition of the vegetable to the steam caused a sharp decline in the temperature of the medium. The return to the boiling point was much more gradual and took a longer period of time. No matter how quickly the vegetables were hmnersed into the steam, considerable steam escaped into X the room before the cover could be replaced on the blanching t container. This and the addition of the cold vegetables were R 21L the causes of the drop in temperature of the steam. Difference in Kgggtables Both broccoli and cauliflower followed the same trends, as indicated above. The differences in blanching times selected probably account for the differences in the internal temperatures reached. 9.4 5*) ( Temperature OF. H x} O 160 wrath 1 Average IIZT.“’1‘11;_1 is?“ 1331; are Trencher? a; arocccli huring slauchinb ‘ ”10:. - .'_ 3.. suT . .. m\\\\\\\\\\\\\\\\\1 is» >3\>>\\>>>§>\>>>>>\> . _.s..ewstxmseven Key 15 min. 3 min. h min. ‘ ,. i din. 7 min. not water ' ote'am elanching Ireutment blanc.7ng -------- Jegetab:e --------- -Range of medium, :‘1':';';:.-'_ umall fiece,[[[flul.arte i-‘iece (.— l... r ryy‘ cur. LII" Y n .0. 8 h.u CY. ai e,n he 1 e; Vial. no t l.g I H e‘l ET mu Eu : .l 18 1“ n0 Tl 8f ti n1 TLU a EU to a T e V 1 l LLO C O I I I . aw ... “wt... {him 0 .mfi. cu.‘f‘.m 5 n. .. .l...d. .1..- o 5.0. 8 o n uusni§n~:s cow. .1“ ...na%o..-.-.uo-..:nuu.uoaens A Wu. 0 3 Lin. tin. A o r 210 200. 190. 7 1 .ho onspshoasma 0 JO 1 r . “—9 t earn «ate t . 110 "L 1 1’15 r , Hui L. l 51 e..- _——-—-—— Large Piece. ——-—-——Veg small riece, .- m Blanch medium, 0/ Key 2' ham 27 Palatability Scores Broccoli The mean scores for the broccoli in each test period are presented in Table l on page 33. General Appearance. With short storage periods (1% and 3 months), the general appearance of the broccoli blanched for the shortest period of time and to the lowest internal temperature was preferred over those blanched for longer intervals. As the blanching time and the internal temp- erature increased, there was some loss in color and texture changes that were detrimental to the general appearance of the vegetable. However, with the four month storage period, although the trend still held for the steam-blanched samples, the scores for the t ree water-blanched samples were closer together and not much preference was indicated. The scores sh wed that tiere was not much deterioration in general appearance of frozen broccoli during short storage periods. The general appearance of the hot water blanched vegetables seemed to be preferred over those vegetables blanched by steam. This was especially true of the longest blanching periods. Throughout the study, the cooked frozen vegetables were compared to freshly cooked vegetables. This was done in two ways: 1.) Comparison with freshly cooked vefetables from the same lot as the frozen samples at the beginning of the 28 n' , Hfinrnir nun. TV... .. i M... ll E 1 ‘ study and 2.) compcrison with fresn vegetables purchased and cooked at each testing period during the study. With the exception of the seven-minute steam treatment, frozen broccoli compared favorably_with the general appearance of the freshly cooked vegetable from the same lot. The setting of the green color during the blanching period acts as a factor in preserving a good appearance. The fresh g broccoli, purchased and cooked during the stuoy and used as ; a control, was a different structural appearance and not of equal fresh quality wit: the broccoli frozen for storage at the beginning of the study. The general appearance of frozen broccoli was rated higher than the fresh broccoli purchased during the winter months of the study. legg. Since color probably was one of the largest factors used as a criterion in Judging general appearance, many of the same trends were observed in the ratings. The lowest scores Occurred in the ratings of the color of the vegetable blanched for the longest period of time in both media. Long blanching periods produced undesirable changes in color, turning the color to an almost olive green in the case of the broccoli blanched for seven minutes in steam to an internal temperature of 2120 F. The low score given to the five-minute steam-blanched vegetable during the four month storage testing period, can be traced to a prejudice of one of the Judges against this sample. 29 Storage for four months had little affect on color. Most of the samples maintained approximately the same score throughout the storage periods. Hot water blanching was better for producing desirable cdlor than was steam blanching. The color of the frozen vegetable rated hiéher than the celer‘ of fresh broccoli purchased during the winter months from the local market. Flavor. The flavor of the broccoli blanched for 1% minutes in hot water to an internal temperature of 180.50 F. was judged the highest during all storage periods. The mean scores which this sample received were closest in value to the mean score given the fresh broccoli at the beginning of the study. For short storage periods, longer blanching periods do not appear to be necessary for flavor retention. The hot water blanch was more desirable than the steam blanch for maintaining the flavor. The longer time intervals used in the steam-blanching could be responsible for greater loss in flavor. Storage for this short period of time had little effect on flavor. The vegetables blanched to an internal temperature closely approaching that of boiling water lost more of their flavor during storage than the vegetables blanched to a lower temperature. By their scores, the judges indicated a preference for the flavor of frozen broccoli over the vegetable purchased 30 and cooked as a control during the study. Again, it must be remembered that the structural characteristics of the broccoli were different except for the last storage period. The stalks and clusters of buds were comparatively smaller and different in appearance. gggg. The shortest blanching period in hot water seemed to be best for preserving desirable odors. The greatest drop in odor ratings during storage occurred in the 22 minute steam blanched sample. Results of enzymatic tests showed that the peroxidase enzyme was not inactivated in this sample. Longer storage periods might show an even greater decline in the scores given odor. Storage seemed to have little effect on the odor of the other samples. The palatability scores for odor indicate that the odor of hot water blanched vegetables was preferred to steam blanched broccoli. None of the scores for odor compared to the scores given to the freshly cooked samples at the begin- ning of the study. However, the odor was rated higher than the vegetables purchased during the study. Texture. Throughout the study, the broccoli blanched to the lowest internal temperature generally rated higher than did the broccoli blanched to higher internal temperatures. Although the cooking times were shortened for the vegetables that were blanched for the longer times, the variation in texture was still noticeable. Storage had little effect on texture scores. Most of 31 If“ "“" the vegetables maintained approximately the same texture scores during the entire study. whether the vegetable was blanched in hot water or steam produced little difference in the scoring results. In comparison to freshly cooked broccoli, the texture of frozen cooked broccoli was either equal or slightly beIOW. Freezing apparently did cause some modification in texture desirability. General Conclusions. The scores given under general conclusions were a reflection of he trends witnessed in the judging of the other factors. For short storage periods, the broccoli blanched in hot water for one and one half minutes was preferred consistently over the other samples. Scores given to broccoli blanched in hot water were on the whole, higher than scores given to comparable samples blanched b7 steam. Storage had little effect on the scores given to the vegetables, with the exception of broccoli, blanched in steam for 5 minutes. A prejudice by one of the judges lowered the mean score considerably at the h month storage period. The frozen broccoli did not rate as high as the fresh broccoli judged at the beginniig of the study. However, it rated higher than fresh broccoli purchased from the local market during the winter months. firaauf.~_’lw-.__(::_:i;." b .-.... Table 1 Mean Scores for Treatments — Broccoli Kean Scores Fresh Broccoli - Before Freezing Gen'l Color Odor Flavor Texture Gen'l Appear. Concl. g.9 6.1 5.9 5.6 5.6 5.7 Treatment Av.int. Gen'l Color Odor Flavor Texture Gen'l temp. Appear. Concl. 1% months storage Fresh(control) h.3 h.7 L.6 “.0 4.g t g Blanched & Frozen Hot water OF. * 1% minutes 130.5 5.? 0.1 5.6 5.h 5.1 5.h 3 minutes 200.3 6.0 5.9 S.h 5.0 5.0 5.1 5 minutes 203.8 5.0 “.h 5.3 5.2 5.0 L.9 Stems 2% minutes 137.0 5.h 5.5 .1 5.0 5.3 5.0 5 minutes q0.31.0 5.3 3.4 W.L 5.0 4.9 5.0 7 minutes 212.0 H.2 L.2 M.7 H.3 L.2 fl.2 3 months storage Fresh(control) 1.6 b.C ),g “.1 3.9 h.l Blanched E Frozen Hot water 1% minutes 190.5 {.0 6.1 5.5 5.5 5.1 5,5 3 minutes 200.3 “.d s.7 §.: 6.3 h.9 5.3 5 minutes 208.8 1.9 5.5 h.8 5.2 ”,0 5.1 Steam 2% minutes 137.0 5.5 5.6 h.0 3. h.8 6,1 5 minutes 2031.0 5. 3.8 5,2 5,1. 73,2 5,3 7 minutes 212.0 h.~ h.3 h.6 L.7 h.§ h.6 h months storage Fresh(control) h.9 5.5 G,1 5,3 g,g _,o Blanched & Frozen Hot water 1% minutes 180.5 5.7 6.1 5.5 5.7 h.9 5.L 3 minutes 200.3 6.0 6.2 h.9 h.s A, 3,6 5 minutes 208.8 5.3 5.9 5.h h,9 h,Z 1,9 Steam 2% minutes 187.0 5.3 5.3 h.5 ).3 5.1 5.1 5 minutes 20h.0 h.8 h.6 h.0 h.h L.O h.1 7 minutes 212.0 5.1 h.8 h.h L.5 h.é 1.5 33 Cauliflower The mean scores for cauliflower in each test period are preserved in Table 2 on page 39, General Appearance. The scores of cauliflower displayed different trends than did broccoli. The medium blanching period was consistently favored over the long and short periods for both steam and hot water. The internal temp— erature of the cauliflower was related to the blanching time, showing a low, medium or high value with a short, medium or long blanching period. Both short and long blanching periods seemed to produce deleterious changes in the general appearance of the cauliflower. The four minute hot water blanch for cauliflower to an internal temperature of 208.00 F. rated the hinhest, when general appearance was concerned. Using steam or hot water apparently produced little difference in the mean scores for comparative blanching periods. Nor did storage for the length of time used in this study produce undesirable changes in general appearance. In general, frozen cauliflower did not rate as high as freshly cooked cauliflower. This applied both to the fresh cauliflower cooked at the beginning of the study and to that purchased from the local market during the study. Color. As with broccoli, the color of cauliflower apparently was the important factor in judging general appearance. As the scores for general appearance varied, 3h so did the scores for color. In most cases, the scores varied less than O.h from the scores given general annearance. The same conclusions about the mean scores can be given, as those given above under general appearance. Before cooking, several of the frozen samoles disnlayed a pink discoloration. This was esoecially noticeable in the cauliflower blanched for three minutes in steam. The ninkish tinge was not noticeable in the cooked nroduct. ngg. The scores given odor pivoted around the medium blanching period and medium internal temperature. The long blanching period, using both steam and hot water, nroduced a noticeable lack of distinctive odor. In the Short blanching periods, the odor was not as pleasing as those samnles that had been blanched to the medium internal temperature. ”i Whether steam or hot water was used, there were little 3‘ differences in the range of mean scores between the two blanching methods . Host of the samples deteriorated in odor quality with storage. The greatest dron was w tnessed in the mean scores of the 6 minute steam sample at the four month storage period. In general, the odor of freshly cooked cauliflower Was better than that of the frozen samples. Flavor. At the 1% month and 3 month storage testing periods, the judges indicated by their scores that there was a preference for the flavor of the cauliflower blanched in 35 'I ‘0 '$-.. hot water or for the 6 minute steam interval. At the four-momth storage testing period, the mean scores of all the samples were closer together and considerable lower than the previous storage periods. This was especially true of the water blanched samples. While for short storage periods, water blanching seemed to insure better flavor "Fl 31' retention, longer storage seemed to equalize the scores for o .p‘J. the two blanching methods. The results indicated that long a“ ...u.:- a... - wh-u. blanching periods are not desirable for flavor retention. '. Worth noting was the rather erratic behavior of the 3 minute steam blanched vegetable. For the first two storage periods, the mean score rated the lowest among the samples. At the four month storage period, it was rated better than several of the other samples. During the three month storage testing period, at two out of the three replications, the judges graded this product as being unacceptable. At the four month storage period, it was again rated acceptable. This was the only sample that gave a positive enzymatic test, and the erratic behavior could be attributed to unpredictable enzymatic activity. Of the factors judged for palatability, the scores for flavor were the lowest. Apparently, preparation for freezing and frozen storage altered flavor more than any of the other factors. This was indicated by comrarison with the scores for flavor of the freshly cooked vegetable. The flavor of the freshly cooked vegetable was consistently rated consider- / 3o ably higher than the frozen vegetable. Texture. Texture scores indicated a different trend than the scores for the other factors. Vegetables blanched for the shortest period of time and to the lowest internal temperature in either medium were judged higher than vegetables blanched for a longer period of time. Although cooking time was adjusted to allow for difference in blanching time, the resulting scores indicated that there were still differences in texture with the different blanching treatments. The shorter blanching period gave a somewhat firmer cooked product, which apparently was preferred by most of the judges. Storage affected the texture of the vegetable blanched to a higher internal temperature more than it did the low internal temperature samoles. Uhile texture scores for the vegetables blanched for a short time tended to remain high throughout the storage periods, the texture scores for the medium.and long blanching period deteriorated in storage. In comparison to the fresh vegetables scored at the beginning of the study: texture scores of frozen products were considerably lower. The scores also rated lower than the scores for the freshly cooked cauliflower purchased from the local market during the winter months of the study. _general Conclusions. During the first two storage periods, the water-blanched sam les and the sample blanched in steam for the six minute time interval were judged to be 37 -.'!‘.~n-.~_-' ‘ - t - I ,5 W \ I 1- 9 U 4‘1". 1"- I . 'of better over-all quality than the vegetables blanched in steam.for longer or shorter periods of time. However, by the end of the study, blanching treatment caused small differences in the judges' ratings. Storage, apparently, tended to equalize any differences present early in the storage. This was also noticeable in the ratings between steam and hot water methods of blanching. Early in the study, water blanched samples were judged better than steam-blanched 8am9163. This difference, also, decreased with storage. i JUdgeS preferred freshly cooked cauliflower to frozen r cauliflower. This preference was more noticeable as the length of storage increased. 38 Table 2 Mean Scores for Treatments - Cauliflower Mean Scores Fresh Cauliflower - Before Freezing Gen'l Color Odor Flavor Texture Gen'l Appear. Concl. 5.7 5.7 5’06 507 6.2 5.6 Treatment Av.int. Gen'l Color Odor Flavor Texture Gen'l temp. Appear. Concl. l%-months storage Fresh(control) h.7 h.7 h.5 h.9 5.2 h.7 Blanched & Frozen Hot water OF. 2 minutes 1790+ h.5 8.3 h.5 L.2 5.0 h.1 h minutes 208.0 5.2 5.3 h.g h.5 .0 h.5 minutes 211.8 a.7 h.7 h. h.5 1.7 h.6 Steam 3 minutes _ 192.2 h.7 h.7 h.0 3.2 h-S fi.5 6 minutes 211.h .3 5.1 5.2 h.7 .3 ,.8 8 minutes 212.0 L.2 h.3 h.3 3.8 4.1 3.8 3 months storage Fresh(control) 3.7 5.6 h.8 5.1 5.4 5.2 Blanched & Frozen Hot water 2 minutes 179.h h.h h.6 h.7 h.8 5.2 h.5 1 minutes 208.0 _.3 5.h h.9 h.2 h.h h.h minutes 211.8 b.8 h.9 h.1 h.2 h.6 L.3 Steam 3 minutes 192.2 h-Z h.0 3'8 .1 a.7 .3 minutes 211.h 5.0 5.0 h. i.0 h.1 i.1 8 minutes 212.0 L.2 2.2 h.0 3.5 h.0 3.7 h months storage Fresh(control) u.9 b.8 L.3 5.0 5.0 h.9 Blanched & Frozen Hot water 2 minutes 179.h .h.2 h40 .h.0 Jul. h.8 h40 1 minutes 208.0 5.5 5.5 h.8 3.8 h.h M.2 8 minutes 211.8 h.1 3.7 3.5 2.9 .8 3.h Steam 3 minutes 192.2 h.8 h.7 h.3 3.8 h.5 h.0 6 minutes 211.2 2.9 2.5 3.5 3.b 2.2 3.7 8 minutes 212. h.2 3.9 3.fi 3. h.l 3.0 .41 __._.M p. J ‘flu . r.-. ”1:” Analysis of Variance of the Scores The scores for color, flavor, texture and general conclusions were analyzed statistically to determine whether the variation was due to treatment, storage or replication or to interaction between these three factors. Table 3 on page A3 gives a summary of the analysis. Broccoli Calculations showed that replication caused little difference in the scores of the broccoli. In the case of the ratings of general conclusions, replication was not accountable for any of the variation. The variation attributable to treatment was highly significant for all scoring factors considered. The previous discussion of the mean scores of the different scoring factors, has indicated that the judges showed a preference for samples blanched by hot water for a short period of time. .As the blanching time increased, the scores generally decreased. The preference for hot water blanching over steam blanching would also result in a high F value due to treatment. In the case of flavor and general conclusions, analysis of variance showed that storage caused some of the variation between scorings. Flavor either remained approximately the same or decreased in quality with length of frozen storage. The interaction between storage and treatment was significant for the color of broccoli. The figures indicate ho .‘m'YP‘fi ‘ """ ‘4‘ s_'_ 1 J {3‘ "K: x‘. IF": do iii-T" - ‘ the lack of uniformity between the response of color to different treatments with storage. The greatest variation was observed in the steam-blanched samples. The color of the samples stemn blanched for 2% minutes and 5 minutes showed a drop in value, whereas the values for the other samples remained approximately the same throurhout the entire storage period. The interaction between storame and replication was highly significant for flavor, texture and reneral conclu- sions. Apparently the judges reacted differently to samples presented at the first replication after a comparatively long time away from judging, than they did to subsequent judgings. This significant figure is rather difficult to trace, especially since the F values due to storage and replication are both 10v. Cauliflower Replication was not a statistically significant cause for variation in color, flavor, texture and general conclu- sions scores. Treatment caused highly significant variations in color and texture, and significant variation in flavor. The discussion of the mean scores for color indicated that medium blanching periods in both steam and hot water insured better color in the frozen product than did the long or short blanching periods. On the other hand, the shortest blanching periods were the most desirable for texture quality. hl - P“ 2-: -jmf .i‘ 92' 1 I. ‘.r ‘13}‘35'7; .é'fli“ ' 21:3. E ‘f.’ 1 l . 1'. i . .. ‘l ‘3, ' .i ‘ but. Treatment did not produce as great a variation in the flavor scores as it did for color and texture. Kean scores indicated that low and high internal temperatures at the completion of the blanching period in steam were not desir- able for flavor retention. In th water-blanched samples, the variation was not as obvious. {fl}... . Storage produced hirhly significant variation in texture F scores. With storage, scores for texture decreased in value i and this was reflected in the high F values for this factor. 3 The interaction between storage and treatment was ’ significant for the texture of the cauliflower. This figure indicates the lack of uniformity in the response of the different treatment to storage. Storage had a more dele- terious effect on the texture of the medium and long blanched samples than it did on the short blanched samples. In the author‘s opinion, the lack of any significant figures under general conclusions, reflects the over-all response to frozen cauliflower. hediocre scores indicated that it probably is not a too satisfactory vegetable for freezing. Table 3 Analysis of Variance - F Values .~ .115" Source Color Flavor Texture General Conclusigng Broccoli Treatment 22 . 57* 3 . 07-;:--::- br. 28-;:~::- 7 . 07-::-::- ' Storage .lh 3.85% .21 3.78% Replication .35 .31 '6% .09 T x s a.57v .23 .70 1.28 T X R 2.97 1.31 1.21 1.00 S X R .Oh ..Sh*% 7.21%* 3.28% Cauliflower Treatment 5.31*£ 3.0hfi 7.17%% 2.66 Storage l.8§ 3.30 8.83%» 2.63 Replication 2.2a 3.00 .25 3.30 T x s 1.03 1.76 2.50% 1.36 T x R 1.uo .Ig; 1.16 .Le S X R l.h0 .50 1.00 .93 ha Significant Significant at 11 level at SK level '1 _J_. ' u D“. . (1 _ M} . . Objective Tests Ascorbic Acid determinations _______._.____ _____.__...____ Broccoli. The results of the ascorbic acid determina- tions are presented in Table A on page he. The results indicated that there are general trends in ascorbic acid content with different treatments. Occasional discrepancies in the general pattern were probably caused by errors in sampling. Tests immediately after blanching showed that consider- able ascorbic acid was lost during the blanching process. The longer the blanching was continued, the greater was the loss of ascorbic acid. Storage tended to nullify these differences. During storage, ascorbic acid was lost at a greater rate in the samples blanched to a low internal temperature and for a short period of time. The low value for the 3 minute hot water blanch at the four month storage period was probably due to an error in smnpling. At the four month storage period, the ascorbic acid values for all treat- ments were much closer together. The blanching process caused a greater loss in ascorbic acid than subsequent frozen storage. Freezing tended to maintain the high ascorbic acid content of the fresh vegetable. However, it must be remembered that this was only a four month study. Longer storage might result in greater changes, especially in the samples blanched to a low internal temp- erature. 1:11 Steam blanching seemed to be slightly better for maintaining the ascorbic acid content of the fresh vegetable than did hot water blanching. However, the difference was not great enough to clearly indicate that one medium should be rated higher than the other. The values for the cooked samples are included in the Appendix on page iv. Cauliflower The results of the ascorbic acid determination for cauliflower are presented in Table h on page A6. The extremes in internal temperature reached by the vegetable were not conducive for maintenance of the ascorbic acid content of the fresh samples. Throughout the entire study, the medium blanching period in both steam and hot water resulted in higher ascorbic acid content. The ascorbic acid content for the hot water blanched samples was consistently higher than for comparable steam- blanchcd samples. Storage for four months did not materially decrease the ascorbic acid content of the A minute hot water sample. Samples from the short and long blanching treatment lost considerable ascorbic acid during storage. The ascorbic acid values for the cooked samples are included in the Appendix on page iv. 12-5 ,sgvr.’ \ .‘K‘?m|l.fll.)uov ~ .flf'lfi.’ 1 Q? 3.qu ‘ . ‘1‘. HI. .’l|<40‘.. [I "nu Ascorbic Acid Content - Mg/lOO Gram.(flet Basis) Table 8 Treatment Av.int. Av. Freshly Frozen Frozen Frozen temp. raw blanched l%-mo. 3 mo. 8 mo. Broccoli Hot Water 1% minutes 180.5 102.88 87.75 83.00 85.00 70.33 3 minutes 200.3 102.‘8 77.75 82.00 8.83 80.50 5 minutes 208.8 102.8' 72.00 72.67 2,33 61,33 Steam 2% minutes 187.0 102.88 89.50 10 .0 3 8 2 5 minutes 208.0 102.88 73.50 8i.87 8.8% 72.3% 7 minutes 212.0 102.88 k7.00 68.17 63.33 Zh.33 Cauliflower Hot Water 2 minutes 17 .h SS.O§ hh.15 85.20 hh,60 81.0 h minutes 20 .0 55.05 85.30 80.80 88.90 87.30 minutes 211.8 55.05 7.40 35.00 38.80 33.20 Steam - minutes 192.2 55.05 82.80 28.80 35.0 17.20 minutes 211.h 55.05 hh.lO 53.30 36.2 37.h0 8 minutes 212.0 55.05 39.80 82.90 29.80 27.20 as w“ ”_.- 4. «1 n .T.‘ “WW - Total solids The results of the tests for total solids are presented in Table 5 on page hB. The percentage of total solids in the raw samples were higher than in the blanched or frozen samples. Additional water apparently diffused into the tissues of the vegetable during blanching and for equivalent weights of vegetable this resulted in lower values for the .1. total solids in he frozen vegetables. Concurrent with this was the leaching of certain soluble solids into the cooking water. Treatment and storage had very little effect on the total solid content. host of the variation probably resulted from sampling techniques. In sampling, efforts were made to include the frosted part of the frozen vegetable, but it was difficult to accurately gauge how much should be included with each piece of frozen vegetable. This resulted in differences in total solids va ues. The values for the cooked solids are included in Appendix on page iv. Table 5 Percent of Total Solids Treatment Av.int. Av. Freshly Frozen Frozen Frozen temp. raw blanched l%-mo.. 3 mo. hjmo. Broccoli Hot Water p 1% minutes 180.5 10.19 9.30 .03 9.31 8.31 3 minutes 200.3 10.19 8.88 .10 7.80 8.%3 5 minutes 208.8 10.19 7.8 8.10 7.79 7. 0 Steam 6 2% minutes 187.0 10.19 7.00 8.81 8.9? 3.20 5 minutes 20h.0 10.19 7.9? 7.85 8.5% .3 7 minutes 212.0 10.19 8.21 7.39 8.15 9.17 Cauliflower Hot Water 9 2 minutes 179.8 10.02 8.87 8.01 8.38 8.78 minutes 208.0 10.02 8.28 6.03 7'73 '55 minutes 211.8 10.02 8.18 8.7 8.;O 7. Steam , minutes 192.2 10.02 $.58 Z.g5 8.%g 2082 minutes 211.8 10.02 {.92 . 0 7.1 7.79 8 minutes 212.0 10.02 0.04 7.28 7. 9 . 88 ‘4. I l‘.‘ I , a '- Enzpne tests The results of the enzyme tests are presented in Table 6 on page 51. In evaluating these results, it should be remembered that the test used for the presence of peroxidase enzyme is simply a qualitative one and, like all enzyme tests, has its limitations. The results for broccoli and cauliflower were the same. With both vegetables, the short blanching period in steam was insufficient to inactivate the peroxidase enzyme. The internal temperatures of the steam blanched samples for the shortest period of time were not as low as the internal temperatures for the water-blanched samples for the shortest period of time. Some other factor beside temperature seemed to operate to make the water blanching more effective than steam in inactivating peroxidase during short blanching periods. The results of the test at the close of the study were the same as at the beginning. The presence of peroxidase activity in broccoli blanched for two and a half minutes in steam did not seem to result in detrimental effects on palatability factors or ascorbic acid retention for short storage periods. At the four month storage period, the ascorbic acid content of this sample had drOpped considerably in comparison to other samples. If the storage were continued, the effect of the presence of this peroxidase enzyme might result in more noticeable effects. 1'9 .42.. .u ~5fi.»fi.¢ if. “in... . ,7 ‘0 .. .,.. . o . \ a. . . . H t. . Aw . L . .. . . .. .. 1 \ t 7. . , ‘1 n I?! .I. by?» k i! $I.'-Y , .5450" x» . 9! .VI . u . . , . .. 1.. .. .l. i L , . \ I. I 1% .ttilnflb . . 1.. l n L . .. . IN . ,III. I . . Uh ... . at; .rI 5". 1’4)”. i . «1% a; .3 ”ii-1b. 1 . .) . r . , . . . 1 .. w w.\.. 1 . . AV '1 .1 I Study of the ascorbic acid values and palatability scores for cauliflower showed that with storage, the sample with the positive enzyme activity had lower values than the other samples. In many cases, the lowest values were dis la ed by the 3 minute steer blanched sample. P . 1 50 n! _‘ ‘:_*. __ " I“ ~ " 71-4 . ,- 2 8V . _ .~m 4 A <34 ' A - v 1 b .42.! “a! P' .9: L" ‘4‘)" 7"“ .. 3,. 1 .3» -~‘-\‘“,.. 1A "f Table 8 Peroxidase Blanching Test Vegetable and Average himediately At close of treatment internal after blanching storage period til-2%.) . Cauliflower Raw Positive Positive Hot water blanched 2 minutes 177-d Negative Negative 1 minutes 218.0 Hegative Negative 5 minutes 311.8 Negative Negative Steam blanched minutes 192.2 Positive Positive 6 minutes fill.h Negative Negative 8 minutes 313.0 Negative Negative Broccoli Raw Positive Positive Hot water blanched 1% minutes 130.; Negative Negative 3 minutes 200.3 Negative Negative 5 minutes 233.8 Negative Negative Steam blanched 2% minutes 187.0 Positive Positive 5 minutes 208.0 Negative Negative 7 minutes 212.0 Negative Negative a . .2 . . w‘v .‘n. ylul t f; .AKL C q . i. ‘ .4 . ..k. , 1 $3: . .. A Q - .u . _.f . . \ )1 .,;| .. . I . I 3.2.! :4 T . I, f! ‘ |.I|P ngetrometer readings of texture This was an exeloratory investigation to determine whether the nenetrometer had any nlace in testing the texture of cooked frozen vegetables, and whether the read- ings would comoare with nalatability scores for texture. The results are nresented in Tables 7 and 8 on pages Ed and 55. First, it should be nointed out that penetrometer readings were taken at only one of the three replications at each test period. The average reading and mean texture scores for this particular reelication are nresented in the table. The average score for all the renlieations in that particular test eeriod are also included, but it should be remembered that the average nenetrometer scores cannot be justifiably compared to these values. Cauliflower. The penetrometer scores, as Well as the texture scores, varied with the treatments. The longer the vegetables were blanched in either the hot.water or the steam, the higher the nenetrometer readings became. Possibly, the contact with heat and moisture softened the cell walls in the vegetable to such an extent that the vegetables became comparatively softer. The penetration of the cone of the penetrometer Was a measure of the firmness or body of the vegetable. The general trend indicated the lower values in penetrometer readings were associated with higher average 52 * ii efir .353st ‘ f. -,_..._ _’~C/’ A 5.5%. 1 A _ 2v." -. is?! , A, , A as n7;é£h? v“ ' '4 " " S r. . 33°93 -. .7 F. palatability scores of texture. A? arent v the judges d .1; preferred a more firm vegetable. The blanching nedium.used seemed to have little effect on the penetrometer readings for conoarative blanching times. The time of exnosure and internal tenterature reached seemed to be the detennining factor in variations in readings. The decli.e in texture scores with sterote was not reflected in the nenetr meter readings. The correlation coefficient between the texture scores and the jenetr meter readings was detelmined. The value was ’ O y below the value of the 5g signifi- Q -.k§3. This was slifht cance level. (. 55) More extensive investigation is recommended before the penetrometer can be used as a testinr instrument for deter- mining texture quality in frozen-vegetables. Broccoli. The behavior of the broccoli under the penetration cone was more erratic than that of cauliflower. Penetrometer readings of tie freshly blanched samnles showed that the values did vary with tr,atnent. it the first test period, techniques for working with the nenetrometer had not been sufficiently develoned, so the readings were not very satisfactory. The correlation coefficient of the nenetrometer values (I and the texture scores at the _our—month neriod was -.lh92, This was not statistica 1y significant. Table 7 Comparison of Penetrometer Readings and Texture Scores - Broccoli Treatment Av.int. Av. a range Score for Av.texture tgmn. of readings same sample score 7,._ 1/10 mm Freshly blanched Hot water 1% minutes 190.5 8.3 (§—TO) 3 minutes 200.3 3.0 (7-17) 5 minutes 208.8 1%.? (13—19) Steam 2% minutes 187.0 7.7 (5—11) 5 minutes 20H.0 8.3 (7-11) 7 minutes 212.0 20.0 (10—23) 1% months storage Hot water , 1% minutes 180.5 120.3(120-130) 5.0 3.1 3 minutes 200.3 132.0(120—1 8) S.h 5.0 5 minutes 208.8 127.7(101-116) 3.0 5.0 Steam 2% minutes 187.0 111.7(109-112) 5.5 §.3 5 minutes 20h.0 102.7 (90-118) 6.3 i.9 7 minutes 212.0 123.7(107-138) h.5 h. h months storage Hot water 1% minutes 180.5 73.3 (57—76) L.8 h.9 3 minutes 200.3 109.0 (92-121) 5.0 h.z 5 minutes 208.8 103.7 (99-111) 3.8 u. Steam 2% minutes 187.0 90.3 (70-103) 5.3 5.2 5 minutes 20M.0 115.3(105—126) h-B u.1 7 minutes 212.0 117.0(102-131) h.8 h.5 Table 8 Comoarison of Penetrometer Readings and Texture Scores - Cauliflower Treatment Av.int. Av. & range Score for the Av.texaure tgmp. of readings smue sample score F. 1 7‘) 1Y1 # Freshly blanched Hot water / 2 minutes 17Q.h-10.7 (13-22) h minutes 200.0 §§.3 (3n-37) minutes 211. 8 44.7 (3w-Sl) Steam minutes 192 2 11.3 (9-13) 0 minutes 211.h 9.3 (EQ-EF) 8 minutes 212.0 h9.0 (bk-9;) 1f:months IIOt Va'ater r" F, {.2 2 minutes 179.h 93.3 (72-‘17) 3-2 9-0 minutes 208.0 120.3(123-132) [3.0 E.o minutes 211.8 12.1-8.3(132-198) n8 1.? Steam h v minutes 192.2 89.3 (09-103) 17.3 . minutes 211.2; 112.3 (93-137) ‘7-2 153 8 minutes 2 .2 .0 venue-loo) 1.2..2 I--1 3 months Hot water i 2 minutes 179.h 8;. 7 (“0 121) v.0 f'fi minutes 208.0 97. 3 (92-100) {0} ‘fi'“ minutes 211.8 11;.0. 3(110-176) 1.0 Steam minutes 192.2 90. 7 (63‘100) L.8 %'1 minutes 211.24. :99. 7(110-12J) 1:40 1,00 8 minutes 212.0 8. 3(128“ )6) 3‘3 L. i_ h months Hot water , 1; .8 2 minutes 179-9 80' 7 (02—96) [.5 fi.h b minutes 208°C 113' 0(139 11?) 1I43 3-8 6 minutes 211.8 130.7( 20 13'J) ‘“ Stem ' 0 minutes 192.2 83- 0 (S7 107) %°§ fi,g minutes 11. h 121 0(109"131) i‘3 H.1 8 minutes 212.0 151- 3(120‘170) ' ' _ii SS SCKLAR: AID COHCLUJIOIS This study was conducted to determine the effect of the internal temnerature reached and Jaintaincd by broccoli and cauliflower during the blanching rrocess on the quality at various intervals during subsequent frozen storage. The vegetables were blanched in hot water or steam for different time intervals. Records wer nade of the internal temnerature of the broccoli and cauliflower during the different blanch- ing procedures. At various intervals in frozen storage the vegetables wcr removed from storage and tested for Luality. A judging 7anel scored nalatability, and objective tests were conducted on ascorbic acid, texture and total solids. Jhen different blanching periods were used, there was a difference in the internal temneratures reached by the vegetable. As the blanching time increased, the interna temperature reached by the vegetable also increased. Lhen the longest neriod of time was used, both in steam.and hot water blanching, the temperature of the vegetable and medium were at or very near the boiling noint of water. Results of nalatability Scores and ascorbic acid determinations indicated that for broccoli a short blanching time (1% minutes in water, 2% minutes in steam) and a low n for internal temperature (130.5 and 1717.00 -.) were best 50 - W .- w wail ' 7“ '4' 4' \m" 1" 'x.‘ .2 ‘Afim 'r :92 w . .. 9.. . . .,. . .. . .1 . .a. 2‘ . . a. <. .u $.71 (1v . a. quality retention of broccoli over short storage periods. Blanehinfi in hot water was more satisfactory than blanching in steam. Frozen broccoli retained much of the quality of the fresh product during storage. It rated hither in nalatabil— ity scores than fre°h broccoli nurehased durinc the winter months of the stud? from the local market. The results of the stud" indicate that cauliflower blanched four minutes in hot water or six minutes in steam to an internal tennerature of 203.00 F. and 211.h° F. resnectiVely produced the most satisfactory nroduet in frozen storage over short nerioas of time. Longer or shorter blanchinr tines resulted in lower quality retention. Under blanching resulted in undesirable flavor and odor, probably due to ensffiatic activity. Over blanchin: nroduced loss in odor and flavor. The product also became soggy. For short storage neriods, hot water was the better blanching medium. However, at the end of four months, results indicated little difference in the quality of cauliflower blanched by either hot water or steam. Freezing cauliflower was not as satisfactory for quality retention as was freezing broccoli. The frozen cauliflower rated lower in nalatability scores than the fresh veeetable scored at the beginning of the study and at the different testing eeriods. 57 Exnloratory investigations on the use of the penetro- meter in testing texture in frozen vegetables showed a general relationshie between tenetrometer readings and palatability scores of texture. U thin limits, the lower the nenetrometer readings, the higher the texture score. However, more work is recommended before the penetrometer readings can be used as a criterion for evaluation of texture. 58 U 10. ll. __ xff/‘W'IXIj _L..: .1 4.4,.) r) . 1L1“... . L LAVA Adan, 3n :1 1‘11 I", C., and Stanworth, J. Chan-(363 Occurring During the Blanchin; of fegetables. J. doe. Chem. Ind. 01, (l _ 2) 7o _ 99 Anon. Green Beans. ,uick Frozen Foods 10, (19L?) - .4 Fly . 110. l, ;)l~_)1‘:_. Alrighi, A. L., Joslyn, I. A., and Zarsh, G. L. Enzyme Activity in Frozen Vegettbles. Ind. Eng. Chem. 23, (1930)) 5/)“‘80 Balls, A. K. 'h.e 1% te of Enzvnes in Processed Foods. . . 4. V .~ Fruit Products J 2211? , 33—3). Barnes, 3., Tressler, D. K. s.nd Fenton,F. Effe ct of Different Cooking Cethods on the Fit. C Content of Quick Frozen Broccoli. Food Research 8, (19.”3), l3—'30. Batcheldcr, 3. L., Kirknn trick, I. 3., Stein, K. 3., and harron, I. K. Jffeet of 3caldi115 Iethods of lualit' of Three home-grown Vegetables. J. hone Econ. 39: (l/-7)! 982-286. Bedford, C. L. and Joslyn, h. A. 3nz.,me Acti vitv in Frozen Vegetables, “tr ngbeans. Ind. Eng. Chem. 31,(1939)1 751‘7580 Bellinger, F. and Kethley T. F., Frozen Food Research Program is Launched at Georgia Tech. Food Ind. 20, (1938), BSQ-Sal, )03- )ab. Birdseye, C. The uicL—lreezln’ of Pcrisha ole Foods. Ice and Refrig. 78, (l)?fi ) 7-; i .‘ thre ;uiek Frozen Vegetables Stand Today. Food Ind. 3, (1?. )1), —13- Brown, K. D. Proper Blanehing is sures Good Results. “ H ‘ ll‘ ;uic1: Frozen Foods 6, (lyhhj, 170. a, 10-5+ Campbell, Horace..)olor Deterioration in Peas#3uring Freezing Storage. guiek Frozen Foods 12, (leQ), Ho. 7, 129-132. 18. 19. 211. 25. . Undcsirable Color Change in Frozen Peas Stored at Sufficiently Low Temreratures. Food Research 2, (1937). 55-37. Carlton, H. Home Freuaration of Fruits and Vegetables for the Freezer Locker. Tenn. Exn. Sta. Bul. 108, (1939), 1-11- Clar, J. E. Preservation of Food Eroduets b7 Freezing. N. J. Agric. ix Sta. Cir. Mel, (19fi3). Crafts, A. S. 5on0 Effects of Blanehing. Food Ind. 16, (lQih). Ida-185. Cox, H. J. and Fae Lasters, I. K. Lierosco ic Studies of Tissue of Frozen Fruits and Vegetables. Food Research 1 r1 7, (lead), 13p-13a. Cruess, 3. V. and Smith, I. 3. Better Blanehing Needed. Quick Frozen Foods 8, (1930),.Uo. 12, S7. D° c.ugjhtcrs, K. 3. ”he Flavor and Texture of Frozen Vegetables. ‘uic: Frozen Foods 9, (l9ho), No. S, 50- o7 Diehl, H. C. Progress Re ort on Frozen Pack. Ice and Refrig. 90, (1730). }—‘H)0 . Freezing Preservation and Frozen P9 ck Research. Ice and Sefrig. 13, (19"_O), 191-406. . Camebell, E., and Ber.y, J. A. Freezing of Aldennan Peas. Food Research 1, (1930), 01—71. . Dingle, 11., andB rry, J. A. Enzymes Can Cause Off-Flavors Sven Jhen Feeds are Frozen. Food Ind. 5. (1933), 300-301- Du 3013, C. U. and Tressler, D. K. The Preparation and Freezin of Certain Vegetables in Locker. Refrig. Eng. 39: (19’0): 107‘108. Bunker, C. F., Fellers, C. 3., and Fitzgerald, G. 1. Stability of Vit. C in Sweet Corn to Shir hing, Freezing and Cannin,. Food Desearch 3, (1937), 1.1-5 0. Evers D. F. Frozen wood Industry. Ind. fins. Chem. b.0, (1918), 2251- -22;3. Farrell, K. T. Vitamins Tested in Freezing, Dehydrating and Canning. Huick Frozen Foods 5, (10i3), No. 11, flip-'15, IO. 12’ 210 . and Fellers, C. 3. Vitamin Content of Green Snau Beans. Influence of Freezing, Canning, and Dehydration on the Content of Thiamine, Riboflavin and Ascorbic Acid. Food Research 7, (l9h2), 171—177. Fellers, C. I. The Sffect of Processing on Vitamins in '06 Fruits and Vegetables, a review, .a o. igr. gxo. Sta. 'fi '-' "\ ’7 V'vf: ’ oul. no. 332, (lyJU). Fcnton. F. Nutris t ti e of guick Frozen Foods. Refrig. nng. £2, (1911 u I l , 1 0—112. - and Tressler, D. K. Losses of Vitmain C During Commercial Freezing, Defrostinn, and Cooking of 7 - _r ‘ I 1 ’ Frosted Peas. Food Research 3, (1936), 409—410. Fitzgerald, C. A. Effects of Freezing on Vitamin C Content of Vegetables. Refrig. jng. 37, (1939), 33-39. ghy You Freeze it That Jay. Food Ind. 22, (19505; £31—eo5. and Fellers, C. R. Carotene and Ascorbic Acid Content of Fresh Earkot and Commercially Frozen Fruits and Vegetables. Food Research 3, (1938), 109- 120. Gleim, 3. G., Eressler, D. F., Fenton, F., Ascorbic Acid, Thiamine, Riboflavin and Carotene Content of Asparagus and Soinach in the Fresh, Stored and Frozen States. Food Research 9, (l9hh), h7l-490. Graham, E. The Nutritive Value of finiek-Frozen Foods. Fruit Products J. 21, (1912), 2n3-2ao. Greaves, V. D. and Boggs , M u. irends in Freezin' Preservation of Foods. J. o S Home Coon. 37: (19%;), 23-26. Jenkins, R. R. and Lee, F. A. Tenderometer Readings as an Index of Quality of Fresh Asraragus. Food Research / // 5. (19h0), 1o1-1oo. ,and Tressler, D. K. and Fitzgerald, G. A. Vitamin C Content of Vegetables, VIII Frozen Peas. Food Research 3, (1933), 133‘1M0- Joslyn, M. A. Preservation of Fruits and Vegetables by Freezing Storage. Calif. an. Sta. Circ. 320, (1930)- . Certain Technological isvects of Preservation by Freezing. guick Frozen Foods 1, (193o), No. 2, 10—13. s1 T . Inzyme Activity, Index of iuality in arogeo Vegetables. Food Ind. 18, (1916), 1204-1310, 133d.- and Marsh, G. L. Changes Occurring During Freezing and Thawing of Fruits and Vegetables. Calif. Agr'l. Ex“. Sta. Bul. 351, (1933). and . Blanching Vegetables for Freezing Preservation, Food Ind. 10, (1933), 379- 3131. 11.39. Kaloyereas, S. A. The Effect of Various hethods of Blanching on Ascorbic Acid and Soluble Solids in Cauliflower and Soinach, Effect of Rapid Freezin« on Catalase Test. Fruit Products J. 25, (19h7), l34-l35. Kertesz, Z. I., Dearbern, 3. 3. and Hack, G. L. Vitamin C in Vegetables./IV. Ascorbic Acid Oxidase. J. 3101. 01102.2. 11'»), (1)30) , 717-725. Kohman, E. F.’ Freezing of Fruits and Vegetables. Ice and Refrig. 7c, (1939), 113—110. . Preservation of Iutritive Values of Foods . ._ . .. .. . .. , v”) 0 In Proces31ng. d. an. nee. assoc. 110, (1912), J31—038. and Sanborn, W. h. Lffect of Resniration on Vegetable Flavor. Ind. Eng. Chem. 20, (193;), 773-770. Lampett, L. F., Baker, L. C. and Parkinson, T. T., Vitamin C Content of Vegetables. II. Effect of Storage on Raw Vegetables. III. Average Figures for Raw Vegetables. J. Soc. Chem. Ind. on, (thS), 200-203. Lee, F. A. The Influence of Smnoling on the Accuracy of the Tenderometer. The Canner 39, (1939), 12, 13. Lineweaver, T. T. Jhe Energy of Activation of Enzyme Reactions and Their Velocity Below 0°. J. :1. Chem. .. v f. sec. 01, (1939), uO3-Hu3. Loeffler, H. J. and Ponting: J. D. Ascorbic Acid, Rapid Determination in Fresh, Frozen, or Dehydrated Fruits and Vezetables. Ind. Eng. Chem., Anal. jd., (19h2), She-819. L: - Magoon, C. A., Keeping 3uality as Related to the o u , . r) 1 Distribution Problem. Ice and Refrig. o0, (1931), 39-t1- ~.1.z\.' .V v I‘ (I .I . U1 \n o mahoney, C. 1., Sallis, 3. F., Hunter, H. 1., and 5 ‘1 .I- ~ \ ~ - . a acctt, L. A. Vitamin Content 01 Ieas, Effect of Freezing, %§g Canning, and Dehydration, Ind. Eng. Chem. 38, (19:0), CSL- -QE 7 .3*~,‘ Masure, M. P. and Campbell, H. Rapid fistbzation of h${;f Pero::idase in Vegetable thracts, a1 Index of Blanching {7 Adeouate for Fiozen Vegetaolefl 0. Fruit Products J. 23, _. 7 (191:), 3.: 371, 333. "9' u n u . . . fiu‘ no 'regor, m., anu Ceuford, C. L. Scalding Vegetables ?§a} Necessarv for Freezing. Quick Frozen Foods 9, (19h?) 9* 1‘“ No. e. 130. ”"“' me Intosh, J. Vitamin C Content in Fresh and Frozen Vegetables. ;uick Frozen Foods 6, (1W.1 , No. 13 311. I)! 9 G+s . The Effect of ?reaaration for Freezing, Freezing Storage and Coolzin~ on tlie Vitamin Content of Vegetables. ‘Fruit Lroducts J. 33, (19 1;-), 113 -ln§, 1.9. , Tressler, D. K. and Fenton, F., The Effect of Different Cooking Lethods on the Vitamin C Content of yuick Frozen Veietables. J. Home Econ. 3h, 3lh—318. Kelnick, D., Hochberg, i. and Bernard, L. 0. Comparative Study of Steam and Hot Hater Blanching. Food Research 9, (lgbiL) , lst-ljBO Mergentime, L. Control Lethods for scalding Vegetables for Freezing. guicy Frozen Foods 1, (1939), No. v , 1}+‘1S, 31.0- 11, 1.2, 11.00 Phaff, H. J. and Joslyn, L. A. Peroxidase Test for Blanching liequires Careful Arrlication. Food Ind. 15, (19:3) , 50-220 Phillips, M. G. and Fenton, F. ffcct of home Freezing and Cooking on Snap Beans. J. H ‘e Econ. 37, (1913), Flagge, H. F., Fruits and Verctables — Studies Relating to l‘heir Freezing and storag Ice and Refrig. 9}, (1938), 220-22 3. . Freezing, Preservation of Fruits and Vege- tables. 'uick Frozen +oods l, (1933), 110. 1, 39. . Four Lessons for Refrigerated Loclcer Patrons in Freezing Vegetables and Fruits. Ice and Refrig. 109, (1993): 1)l-2 70- 71. f) t—o 73. 74. 7S. PPOCtOP, 3.‘ dimnle fists Reveal Injroner Blanehing. Food Ind. L1, (1912), 31-52. Retzer, J. 1., Van Duyne, F. 0., Chase, J. T. and Simnson, J. I. jffeet of Steam and hot Eater Blanehing on Ascorbic Acid Content of Snap Beans and Cauliflower. Food Research 10, (1015), 518-53 . Robertson, 5, F. Jhy and How to Blanch. ,uick Frozen Foods 7, (1945), :0. 11, 90. . and Cruess, E. H. The Oxidase of Rosoff, Harold D , | | Food Research la, (1949), 282. Cauliflower. Smart, H. F. and Brunstetter, B. C. Lima Beans in Frozen Pack. I. Blanching Tests. II. Iicrobiological I / Studies. The Canner S3, (1930), lh-lo. and . Srinach and Kale in Frozen Pack. I. Scalding Tests. IT./hierobiological Studies. Food Research 3, (1937), 131-103. Strasburger, L. V. Some Notes on Blanching Peas. The -7 I Canner 7S, (1932), no. 2h, 13-14. Todhunter, L. H. and Robbins, R. C. Ascorbic Acid (Vitamin C) Content of Garden Tyne Teas Preserved by the Frozen Pack lethod. Has}. Agr. Exn. Sta. Bul. 1108, (191:1). Todhunter, B. N. and Searding, B. L., Vitamin Value of Garden Tyne Peas Preserved by Frozen Fack‘uethod. I. (1 _ Vitamin C. Food Research 3, (l93o), 439-49o. Tressler, D. K. Chemical Problems of the Suick-Freezing Industry. Ind. Eng. hem. 21, (1932), 632-686. . 1uality Control Vital to Success inJFrozen . Changes Ihieh Kai Occur in Frozen Foods During Cold Storage. Food Ind. 5, (1933), 336. Tressler, D. K. and Evers, C. F. The Freezing Preservation of Feeds. The Avi Publishing Comnany, Inc. New York, 19h7. Tressler, D., hack, G. L. and King, C. G. Factors Influencing the Vitamin C Content of Vegetables. J. Am. Pub. Health Assoc. 26, (193o), 903-909. . . I, v 4 J . i L 1 ' d‘ {" n - J #4,: .r - b I -) ‘n L {1 I .K, 1 l}. ’0 K A 3; , ,7 "1‘! c!“ ‘3‘} Tu F.‘ 1‘ f ‘1’: Tressler, D. X., hack, 9. L, Jenkins, R. R. and King, C. G. Vitamin C in Vegetables. VII. Lina Beans. Food Research 3, (1937), 179-131. Western Regional Laboratory, Test for Ldequacy of Blanching in Frozen Vegetables. Inf or.1abion Sheet from the ester. Regional Laboratorv, Albany, Calif., Bureau of Agricultural and Ind. Chem., Agr‘l Res. Ac D. A. 'f I‘ .1. ’ L}. Q. fiheeler, Katherine and Tressler, D. K. ”lit uzin C Content of Vegetables. KII. Broccoli, Cau aliflower, Endive, C ntaloup, Parsnirs, Few Zealand Sninach, Kohlrabi, Lettuce and1_ale. Food Research 1, (1939), 593—604. Uilmot, J. 1Wuritivehf.lue and Ireparo tion of Frozen Foods. Refrig. Lngin. 3o, (1939), 7w-JO. Uintor, J. D. and Instrulid, A. Freezing Foods for Home Use. U. of Xinn. Agr'l Ext. Serv1ce. Ext. Bull. 21111., (17.11;) . Hoodroof, J. G. Prescrveam wmo go mwwwhom haw ”noneam poem haw» I H .wmondeaoem no: node awesome .mhoeo adhdpeeq: heave so emuflcwHo ”Mono soon I N ultllu h .cowumnoaoowfie mango Sum .mefinsopn .mmmcaase .wuflamxeme no wanesm upoaou EdHUHMM m .1: . . .1 . u . _.1unxnm . doom 1 m nofiunom vfiaom Seem wwdofia mo cowp31mtmm woes: mamas; 2H one doom ahm> Io .mefiMw mo mnfiflmzoam .mcwwmmaaoo .wuwaxewhx .mcfignfihdm odes: ”moqehweggu prwcea pceaaw)xq M c n "hapeHSQthwm woo: up upommom hem meaeoom Ho mewloanwpmeoo< nofimfiHocoo Hesmnea ondpxma nobaflh HOUO hoaoo .moz mSSamm Score Card Used For ”udgi'Ig Paltibility ween IlllllllllllllllllllllIIIIIIIIII. eemz weapopewo> dad muflsnm uwmohm Mom chem ogoom Internal Temperature Reached and Time Heintained by Broccoli During Blenching Treatment OF OF OF Time OF medium. small minutes large minutes cooled piece niece media 1% minutes 206-208 200 160 60- 0 206-208 176 1Z0 58-68 206-208 176 1 0 56-66 3 minutes 206-210 210 202 58-66 206-210 210 208 62-70 206-210 208 161 206-210 208 172 56-71 5 minutes 206-212 212 206 52-5h 206-212 212 206 5h-56 206-212 212 208 52-52 208-212 212 210 54 2% minutes 1 196—190 180 138 Sh-GB 2 166-206 202 188 20-32 E 178-210 206 172 66-68 1 166-206 176 178 50-58 5 166-208 200 182 60-62 5 minutes 1 156-212 212 208 1/1 5b-56 2 161-212 212 212 3/6 52-56 166-210 208 202 56-70 i 120-208 178 18 58-60 5 132-208 202 178 58-62 7 minutes 1 160-212 212 212 2-1/6 56-58 2 160-212 212 212 1-3/6 6-58 P 160-212 212 - 212 1-1/2 56-62 1 158-212 212 212 2-3/1 56 5 151-212 212 212 1-3/t 56-58 Internal Temperature Reached and Time Maintained by Cauliflower During Blanching Treatment or OF Time 0F Time OF medium small minutes large minutes cooled piece piece media Hot Water 7 2 minutes 1 206-208 181 166 18 2 20L-206 188 156 38-88 3 206-208 208 1 178 50-62 h minutes 1 206-212 208 202 56-56 2 206-212 212 1-1/8 206 58-60 3 206-212 212 3/8 208 51-86 6 minutes 1 208-212 212 1-1/2 212 1-1/2 58-62 2 206-212 212 3-1/8 210 52-5 P 206-212 212 1 212 . 3/6 52-5 1 208-212 212 1-1/2 212 1-1/2 58-62 Steam 3 minutes 1 166-210 202 1 1 56-60 2 160-208 186 168 56-58 P 161-210 200 176 54-56 1 loo-210 172 188 51-56 g 161-210 202 200 52-5% 162-212 210 176 -5 7 166-210 206 202 5 -60 6 minutes 1 166-210 210 1-1/2 208 1/2 5 -58 2 186-212 212 2-3/h 212 2-3/h 5 -60 i 160-212 212 212 58-60 160-212 212 2 212 58-62 5 162-212 212 2-3/1 212 2—3/1 58-62 8 minutes 1 156-212 212 5 212 8-1/2 60-66 2 168-212 212 /f 212 3-3/1 58-62 3 158-212 212 8-1 1 212 3-1/2 60-62 1 168-212 212 u-l/u 212 1-1/8 60-62 5 168-212 212 1 212 1-3/1 60-62 Ascorbic Acid and Total Solids - Cooked Samnles Treatment 1% months 3 months Qimonths solids liquids solids liquids solids liquids Broccoli Hot Tater 1% minutes 10.75% 3.18 36.50 30.6 19.00 18.10 7.171% 1.10 8.51 1.61 7.07 1.75 3 minutes 35.13 8.75 33.63 23.50 35.75 8.10 8.20 1.75 8.52 1.36 8.71 1.51 5 minutes 30.75 7.38 11.25 7.10 16.75 8.85 7.18 1.21 8.73 1.25 7.18 1.20 Steam , 2% minutes 13.03 7.23 11.38 18.30 12.88 18.10 7.71 1.15 8.30 1.16 7.87 1.61 5 minutes 37.38 8. 5 11.50 16.30 13.13 12.80 8.10 1.11 7.01 1.57 8.18 1.51 7 minutes 33.25 1.25 37.75 23.50 18.25 13.15 7.52 2.10 8.72 1.1 8.18 1.11 Cauliflower Hot Water 2 minutes 28.88 6.60 20.38 5.38 17.67 1.70 6.75 1.10 7.37 1.21 7.30 1.22 1 minutes 28.21 6.17 11.01 6.16 37.38 5.57 7.81 1.00 7.70 1.11 7.05 1.37 6 minutes 15.75 2.72 13.63 3.71 13.63 3.51 6.78 .85 7.56 1.02 7.68 1.12 Steam 3 minutes 11.57 1.72 12.07 1.71 28.63 3.18 7.27 1.20 8.1 1.36 6.58 1.15 6 minutes 35.71 2.80 25.6 1.78 20.07 1.18 6.01 .77 6.71 1.13 7.21 .22 8 minutes 28.63 5.05 12.88 3.68 18.38 2.13 6.71 .72 .30 1.00 7.21 1.03 - Ascorbic acid mg/lOO gm. xw Total solids - percentage iv 2523M -‘ \. 4 r. ‘ i 41594:.2’ . 1““? .2— _~. .. 311%. t N M'11111711le1![11111171111115s