llllllllllHllllllllilllflllllllllllllllllHlllllllllllfllllll 31293 01579 6018 LITERARY Michigan State University 1 PLACE II RETURN BOX to roman this checkout 1mm your "cord. TO AVOID FINES Mum on or baton date duo. DATE DUE DATE DUE DATE DUE ll 7&3 I | MSU I. An Afflrmdlvo Action/Equal Opponunlly Imam WM! A FAMILIAL STUDY OF GROWTH AND HEALTH-RELATED FITNESS AMONG CANADIAN S OF ABORIGINAL AND EUROPEAN ANCESTRY By Peter Todd Katzmarzyk A DISSERTATION Submitted to Michigan State University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Department of Physical Education and Exercise Science 1997 ABSTRACT A FAMILIAL STUDY OF GROWTH AND HEALTH-RELATED FITNESS AMONG CANADIAN S OF ABORIGINAL AND EUROPEAN ANCESTRY By Peter Todd Katzmarzyk The purpose of this study was to compare Canadians of First Nation (FN) and European ancestry (BA) in terms of body size, physique, and indicators of health- related fitness, and to determine the familial resemblance in these variables. A total of 624 subjects, 130 FN, 494 BA from the Northern Ontario communities of Temagami and Bear Island participated. The results indicated significant differences between FN and EA Canadians, and significant familial resemblance in body size, physique and health-related fitness. Generally, FN subjects were fatter and had a more central subcutaneous fat distribution than EA subjects. In both groups, males had less subcutaneous adiposity, but had a greater tendency to store proportionally more fat on the trunk than females. Few differences were evident for stature and skeletal dimensions between FN and EA subjects. The results also indicated that FN subjects were more endomorphic than EA subjects. The prevalence of obesity in FN was generally higher than in BA. Among males and females 5- 19 years, the prevalence of obesity (285th percentile age-specific NHANES II BMI) was 38.1% and 29.4% in FN males and females, respectively, and 21.3% and 16.9% in BA males and females, respectively. In FN adults 20—75 years, the prevalence of obesity (285th percentile NHANES II BMI for 20-29 year old people) was 51.4% in FN males, 58.8% in FN females, 39.0% in BA males, and 35.0% in BA females. Analyses of secular changes indicated a positive secular trend for stature of 1.0 cur/decade in EA males. Estimated secular changes in the other groups were not significant. Correlations between first degree relatives indicated significant familial resemblance in body size, physique, adiposity, relative fat distribution, grip strength and trunk flexibility. Spousal correlations showed little assortative mating in this sample. The results suggest that the increased prevalence of several metabolic diseases in FN Canadians may in part be explained by morphological characteristics which are associated with increased risk for disease, and that these differences are apparent in childhood. For Brenda and Bradley iv ACKNOWLEDGMENTS Any project of this magnitude is a team effort. I would like to thank all of those who have assisted me in this endeavor. Firstly, this project could not have been completed if it were not for the participants. I would like to thank the residents of Temagami and Bear Island for taking the time to participate in this project. In particular Wayne Adair, Reeve of Temagami, Holly Charyna, past Chief, and J immy Twain, current Chief of the Temagami First Nation, made special efforts in getting this study off of the ground. Special mention must also be made of the Education Committee of the Timiskaming Board of Education and the teachers of the public schools in Temagami and Bear Island for their assistance during data collection. Within the academic realm, I would like to thank first and foremost Dr. Robert Malina for his guidance and friendship throughout the past three years, which allowed me to complete my degree in a timely manner. I would also like to acknowledge the efforts of my dissertation committee; Drs. Crystal Branta, Sharon Hoerr, and James Pivamik for their constructive criticism and commentary. Special thanks also to Dr. Claude Bouchard for providing comparative data from the Quebec Family Study. Last but definitely not least, I would like to thank my wife Brenda for her support throughout this long journey. Also, Brenda has acted as an unpaid research assistant throughout the entire research process. Without her efl'orts, I am sure I would still be in the field collecting data. TABLE OF CONTENTS LIST OF TABLES ........................................................ ix LIST OF FIGURES ..................................................... xiv CHAPTER I INTRODUCTION AND CONCEPTUAL FRAMEWORK Introduction ................................................................. Rationale and Purpose of Study .......................................... 3 Research Hypotheses ...................................................... 4 Limitations of Study ....................................................... 4 Significance of Study ...................................................... 5 CHAPTER 11 REVIEW OF RELATED LITERATURE Introduction ................................................................. 6 Section 1: Growth and Adult Characteristics of Native North Americans Introduction ................................................................. 6 Growth Data ................................................................ 8 Body Size ................................................................... 8 Fatness and Relative Fat Distribution .................................. 1 l Physique .................................................................. l6 Secular Trends ........................................................... 17 Summary .................................................................. 19 Section II: Familial Aggregation of Body Size, Physique, and Indicators of Heath-Related Fitness Introduction ................................................. ~ .............. 20 Types of Studies ......................................................... 20 Body Size ................................................................. 22 Circumferences and Skeletal Dimensions ............................. 24 Fatness and Relative Fat Distribution .................................. 26 Physique .................................................................. 28 Strength and Flexibility ................................................. 29 Summary .................................................................. 30 vi CHAPTER III METHODS Introduction ............................................................... 31 Research Location and Study Population ............................. 31 Ethnographic Background .............................................. 32 Study Design ............................................................. 34 Sample ............................................................. q ....... 3 5 Anthropometry ........................................................... 36 Anthropometric Indices ................................................. 40 Strength, Flexibility and Motor Fitness ............................... 42 Measurement Variability and Reliability ............................... 44 Data Management ........................................................ 46 Statistical Analyses ...................................................... 48 CHAPTER IV RESULTS Introduction ............................................................... 53 Descriptive Statistics ..................................................... 53 Anthropometric Z-Score Analysis ..................................... 61 Secular Trend Analysis for Stature .................................... 63 Stature, Skeletal Dimensions and Circumferences ................... 64 Body Mass, Fatness and Relative Fat Distribution .................. 66 Prevalence of Obesity ................................................... 68 Physique .................................................................. 70 Grip Strength, Trunk Flexibility and Motor Fitness ................. 73 Interrelationships Among Body Size, Fatness, Physique and Health-Related Fitness .................................. 75 CHAPTER V DISCUSSION Introduction ............................................................... 77 Stature and Skeletal Dimensions ....................................... 77 Fatness and Relative Fat Distribution .................................. 80 Prevalence of Obesity ............................... , .................... 84 Physique .................................................................. 88 Grip Strength, Trunk Flexibility and Motor Fitness ................. 92 Secular Trends ........................................................... 94 Body Size, Fatness, Physique and Health-Related Fitness ......... 98 CHAPTER VI SUMMARY AND CONCLUSIONS Summary ................................................................. 107 Conclusions ............................................................. 1 1 1 Recommendation for Future Research ............................... 114 TABLES ...................................................................... 116 FIGURES ..................................................................... 171 vii APPENDIX A: COMMUNITY CORRESPONDENCE ........................ 298 LIST OF REFERENCES ............................................. 301 viii LIST OF TABLES TABLE 2.1 Comparison of stature, mass and estimated BMI among selected samples of adult Native North Americans. BMIs are estrmated from means for stature and mass in each sample .................. 116 TABLE 2.2 Components of health-related fitness .......................... 117 TABLE 2.3 Evidence for familial resemblance in stature .................. 118 TABLE 2.4 Evidence for familial resemblance in body mass ............. 119 TABLE 2.5 Evidence for familial resemblance in circumferences ........ 120 TABLE 2.6 Evidence for familial resemblance in skeletal dimensions . . 121 TABLE 2.7 Evidence for familial resemblance in the BMI ................ 122 TABLE 2.8 Evidence for familial resemblance in fatness and relative fat distribution ............................................................. 123 TABLE 2.9 Evidence for familial resemblance in somatotype ............ 124 TABLE 2.10 Evidence for familial resemblance in grip strength and trunk flexibility ............................................................... q. 125 TABLE 3.1 Age and sex distribution of subjects compared to reported populations of Temagami and Bear Island ........................... 126 TABLE 3.2 Age and sex distribution of the subsample . participating in the analysis of measurement variability ....................... 127 TABLE 3.3 Mean differences (3,), intraobserver technical errors of measurement (TEM) and intraclass correlation coefficients (fl...) between replicate measurements (n=64) ........................................ 127 TABLE 3.4 Comparison of intraobserver technical errors of measurement (TEM) with those reported in selected studies ................. 128 TABLE 3.5 Sample sizes, age ranges, and intraclass correlations (Ti...) for replicate motor performance tests ..................................... 129 ix TABLE 3.6 Comparison of reliability coefficients for replicate performance tests .................................................................. 129 TABLE 3.7 Least squares regression equations for the prediction of individual skinfolds in a sample of Canadians .............................. 129 TABLE 3.8 Skewness statistics for variables with skewed distributions and skewness statistics after log ,0 transformation of the variables... ........ 130 TABLE 3.9 Effects of age, by gender, on skeletal dimensions, circumferences, and AMA ........................................................ 131 TABLE 3.10 Effects of age, by gender, on fatness, relative fat distribution, physique, grip strength, and trunk flexibility ................... 132 TABLE 3.11 Distribution of sibship size among 266 families ........... 133 TABLE 3.12 Effects of age, mass, stature and the BMI, by gender, on grip strength and flexibility ...................................... 134 TABLE 4.] Sample sizes, means and standard deviations for age and indicators of body size .............................................. 135 TABLE 4.2 Sample sizes, means and standard deviations for indicators of fatness and relative fat distribution ............................... 136 TABLE 4.3 Sample sizes, means, medians, and standard _ deviations for extremity skinfolds ............................................... 137 TABLE 4.4 Sample sizes, means, medians, and standard deviations for trunk skinfolds .................................................... 138 - TABLE 4.5 Sample sizes, means and standard deviations for skeletal breadths ............................................................... 139 TABLE 4.6 Sample sizes, means and standard deviations for circumferences and AMA ..................................................... 140 TABLE 4.7 Sample sizes, means and standard deviations for Heath-Carter anthropometric somatotype components .................... 141 TABLE 4.8 Sample sizes, means and standard deviations for grip strength and trunk flexibility .......... ‘ .................................. 1 42 TABLE 4.9 Sample sizes, means and standard deviations for age and motor fitness in children 5-15 years of age ....................... 143 TABLE 4.10 Anthropometric z-scores"2 and results of t-tests for differences between males and females, and between EA and FN samples ............................................................... 144 TABLE 4.11 Anthropometric z-scoresl for FN adults 20-75 yrs standardized using FN reference data from Canada and results of t-tests for differences between males and females .......................... 145 X TABLE 4.12 Results of the secular trend regression analysis for stature ................................................................. 145 TABLE 4.13 Results of AN COVAs for differences in stature, skeletal dimensions, and AMA between EA and EN subjects, . with age as the covariate .......................................................... 146 TABLE 4.14 Results of ANCOVAs for differences in stature, skeletal dimensions, and AMA between males and females, with age as the covariate ........................................... 147 TABLE 4.15 Intraclass sibling correlations for stature, skeletal dimensions, circumferences, and AMA ............................... 148 TABLE 4.16 Interclass spousal and parent-offspring correlations for stature, skeletal dimensions, circumferences, and AMA .................. 149 TABLE 4.17 Heritability estimates for stature, skeletal dimensions, circumferences and AMA based on regression analyses of offspring on mid-parent values ..................................... 150 TABLE 4.18 Results of ANCOVAs for differences in body mass, fatness, and relative fat distribution between EA and EN subjects, with age as the covariate .......................................................... 151 TABLE 4.19 Results of ANCOVAs for differences in body mass, fatness, and relative fat distribution between males and females, with age as the covariate .......................................................... ‘ 152 TABLE 4.20 Intraclass sibling correlations for fatness, and . relative fat distribution ............................................................. 153 TABLE 4.21 Interclass spousal and parent-offspring for fatness and relative fat distribution ........................................................ 153 TABLE 4.22 Heritability estimates based on regression analyses of offspring on mid-parent values for fatness and relative fat distribution ..... 154 TABLE 4.23 Prevalence of obesity .......................................... 155 TABLE 4.24 Differences between adult subjects classified as obese by different criteria ...................................................... 156 TABLE 4.25 Results of MANCOVAs and univariate F-tests for Heath-Carter anthropometric somatotype components between EA and FN subjects, with age as the covariate ................................. 157 TABLE 4.26 Summary of forward stepwise discriminant function analyses for the pairwise comparisons in the analysis of somatotype differences between EA and FN subjects: Entries show the component entered on each step and the F-value to enter ................................... 158 xi TABLE 4.27 Results of MANCOVAs and univariate F-tests for Heath-Carter anthropometric somatotype components between males and females, with age as the covariate ................................... 159 TABLE 4.28 Summary of forward stepwise discriminant function analyses for the pairwise comparisons in the analysis of somatotype differences between males and females: Entries show the component entered on each step and the F-value to enter ................................... 160 TABLE 4.29 Intraclass sibling correlations for ' somatotype components ........................................................... 161 TABLE 4.30 Interclass Spousal and parent-offspring correlations for somatotype components ...................................................... 161 TABLE 4.31 Heritability estimates based on regression analyses of offspring on mid-parent values for components of somatotype ........... 161 TABLE 4.32 Results of AN COVAs for differences in grip strength, trunk flexibility, and motor performance between EA and FN subjects, with age as the covariate ............................................... 162 TABLE 4.33 Results of ANCOVAs for differences in grip strength, trunk flexibility, and motor performance between males and females, with age as the covariate .......................................................... 163 TABLE 4.34 Intraclass sibling correlations for grip strength and trunk flexibility ................................................................ 164 TABLE 4.35 Interclass spousal and parent-offspring correlations for grip strength and trunk flexibility ............................. 164 TABLE 4.36 Heritability estimates for grip strength and trunk flexibility based on regression analyses of offspring on mid-parent values ............ 164 TABLE 4.37 First-order gartial correlations between body size and fatness, and grip strength, exibility, and performance variables, controlling for age ..................................................... 165 TABLE 4.38 Third-order partial correlations between somatotype components and grip strength, flexibility, and performance variables, controlling for age and the other two somatotype components ............... 166 TABLE 4.39 Results of familial aggregation analyses of flexibility and grip strength examining the effects of controlling for body size ........ 167 TABLE 5.1 Partial correlations between TER, WHR and SUM, controlling for age ................................................................. 168 TABLE 5.2 Results of familial aggregation analyses of TER and controlling for SUM ..................................................... 169 xii TABLE 5.3 Means and, standard deviations for Heath-Carter anthropometric somatotype components with comparative data from two Canadian samples ...................................................... 170 xiii LIST OF FIGURES FIGURE 1.1 General model outlining the hypothesized relationships among activity, fitness and health (adapted from Bouchard and Shephard, 1994).. 171 FIGURE 1.2 Proposed model for the study of the genetics of body size and performance ........................................................ 172 FIGURE 2.1 Stature among selected samples of Native North American males 4-18 years plotted relative to Canadian reference data (Health and Welfare Canada, 1980). Solid lines represent 10th and 90th percentiles .......................................................... 173 FIGURE 2.2 Stature among selected samples of Native North American females 4-18 years plotted relative to Canadian reference data (Health and Welfare Canada, 1980). Solid lines represent 10th and 90th percentiles .......................................................... 174 FIGURE 2.3 Body mass among selected samples of Native North American males 4-18 years plotted relative to Canadian reference data (Health and Welfare Canada, 1980). Solid lines represent 10th and 90th percentiles .......................................................... 175 FIGURE 2.4 Body mass among selected samples of Native North American females 4-18 years plotted relative to Canadian reference data (Health and Welfare Canada, 1980). Solid lines represent 10th and 90th percentiles .......................................................... 176 FIGURE 2.5 Estimated body mass index among selected samples of Native North American males 4-18 years plotted relative to US. reference data (Must et al., 1991). Solid lines represent 5th and 95th percentiles ........................................................... 177 FIGURE 2.6 Estimated body mass index among selected samples of Native North American females 4-18 years plotted relative to US. reference data (Must et al., 1991). Solid lines represent 5th and 95th percentiles ........................................................... 178 FIGURE 3.1 Map of research location in Northern Ontario ............... 179 FIGURE 4.1 Stature of EA males (0) 5-19 years and five-year age group means (A :h SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 180 xiv FIGURE 4.2 Stature of FN males (0) 5-19 years and five-year age group means (A 1 SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 181 FIGURE 4.3 Stature of EA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Canadian reference a data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines rcpresent 10th, 50th, and 90th percentiles .......................... 182 FIGURE 4.4 Stature of FN females (0) 5—19 years and five-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 183 FIGURE 4.5 Stature of EA males (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are :t SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 184 FIGURE 4.6 Stature of FN males (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are 1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 185 FIGURE 4.7 Stature of EA females (0) 20-75 years and ten-year age gzroup means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are i SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 186 FIGURE 4.8 Stature of FN females (0) 20-75 years and ten-year age mup means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles ......................... 187 FIGURE 4.9 Body mass of EA males (0) 5-19 years and five-year age group means (A i SD) plotted relative to Canadian reference . data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 188 FIGURE 4.10 Body mass of FN males (0) 5-19 years and five-year age group means (A i SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. SOlid lines represent 10th, 50th, and 90m percentiles .......................... 189 1FIGURE 4.11 Body mass of EA females (0) 5-19 years and five-year 389 group means (A :1: SD) plotted relative to Canadian reference data (Fatness Canada, 1985). Horizontal bars are :1: SD for age. SOlid lines represent 10th, 50th, and 90th percentiles .......................... 190 XV Fr .Q.‘ 92.14. Insulin FIGURE 4.12 Body mass of FN females (0) 5—19 years and five-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 191 FIGURE 4.13 Body mass of EA males (0) 20—75 years and ten-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are i SD for age. - Solid lines represent 10th, 50th, and 90th percentiles .......................... 192 FIGURE 4.14 Body mass of FN males (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 193 FIGURE 4.15 Body mass of EA females (0) 20—75 years and ten-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles ......................... 194 FIGURE 4.16 Body mass of FN females (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are i SD for age. S olid lines represent 10th, 50th, and 90th percentiles ......................... 195 FIGURE 4.17 Sitting height of EA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Hamill ct al., 1973; Malina et al., 1974). Horizontal bars are :1: SD for age. S (slid lines represent 10th, 50th, and 90th percentiles .......................... 196 FIGURE 4.18 Sitting height of FN males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 197 F1 GURE 4.19 Sitting height of EA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 198 IFIGURE 4.20 Sitting height of FN females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are i SD for age. Solidlinesrepresent 10th, 50th, and90thpercentiles .......................... r99 IFIGURE 4.21 Sitting height of EA males (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to Canadian reference data (Health and Welfare Canada, 1980). Horizontal bars are :r so for age. Solid lines represent 10th, 50th, and90thpercentiles .......................... 200 I“IGURE 4.22 Sitting height of FN males (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to Canadian reference data (Health and Welfare Canada, 1980). Horizontal bars are :1: SD for age. SOlid lines represent 10th, 50th, and 90th percentiles .......................... 201 xvi FIGURE 4.23 Sitting height of EA females (0) 20—75 years and ten-year age group means (A :1: SD) plotted relative to Canadian reference data (Health and Welfare Canada, 1980). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 202 FIGURE 4.24 Sitting height of FN females (0) 20-75 years and ten-year age aggroup means (A :1: SD) plotted relative to Canadian reference data (Health and Welfare Canada, 1980). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 203 FIGURE 4.25 Estimated subischial length of EA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles ............... 204 FIGURE 4.26 Estimated subischial length of FN males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles ............... 205 FIGURE 4.27 Estimated subischial length of EA females (0) 5-19 years and five-year age group means (A i SD) plotted relative to US. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles ............... 206 . FIGURE 4.28 Estimated subischial length of FN females (0) 5-19 years and five-year age group means (A i SD) plotted relative to US. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles ............... 207 FIGURE 4.29 Sitting height/stature ratio of EA males (0) 5-19 years and five-year age group means (A i SD) plotted relative to US. mference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. . . . 208 FIGURE 4.30 Sitting height/stature ratio of FN males (0) 5- 19 years and five-year age group means (A :1: SD)p lotted relative to U. S. reference data (Hamill et al., 1973; Malina et al.,1974). Horizontal bars are :1; SD for age. Solid lines represent 10th, 50th, and 90th percentiles... .209 FIGURE 4.31 Sitting height/stature ratio of EA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to U. S. reference data (Hamill et al., 1973, Malina et al.,1974). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles... 2.10 Fl(IURE 4.32 Sitting height/stature ratio of FN females (0) 5- 19 years aIncl five-year age group means (A :1: SD)p lotted relative to U. S. lfifterencre data (Hamill et al., 1973; Malina et al.,1974). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles... .211 xvii FIGURE 4.33 Sitting height/stature ratio of EA males (0) 20—75 years and ten—year age group means (A :1: SD) groups plotted relative to Canadian reference data (Health and Welfare Canada, 1980). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. . .. 212 FIGURE 4.34 Sitting height/stature ratio of FN males (0) 20-75 years and ten-year age group means (A 1 SD) plotted relative to Canadian reference data (Health and Welfare Canada, 1980). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. . .. 213 FIGURE 4.35 Sitting height/stature ratio of EA females (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to Canadian reference data (Health and Welfare Canada, 1980). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles.... 214 FIGURE 4.36 Sitting height/stature ratio of FN females (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to Canadian reference data (Health and Welfare Canada, 1980). Horizontal bars are :1: SD for age. Solid lines represent 101b, 50th, and 90th percentiles. . . . 215 FIGURE 4.37 Estimated arm muscle area of EA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Frisancho, 1990). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 216 FIGURE 4.38 Estimated arm muscle area of FN males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Frisancho, 1990). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 217 FIGURE 4.39 Estimated arm muscle area of EA females (0) 5-19 years an d five-year age group means (A 1 SD) plotted relative to US reference data (Frisancho, 1990). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 218 FIGURE 4.40 Estimated arm muscle area of FN females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Frisancho, 1990). Horizontal bars are :1: SD for age. Solidlinesrepresent 10th, 50th, and90thpercentiles .......................... 219 I“I(}URE 4.41 Estimated arm muscle area of EA males (0) 20-75 years and ten-year age group means (A :1; SD) plotted relative to US. reference data (Frisancho, 1990). Horizontal bars are :1: SD for age. Sclid linesrepresent 10th, 50th, and90thpercentiles .......................... 22o I‘~I(IURE 4.42 Estimated arm muscle area of FN males (0) 20-75 years a11d ten-year age group means (A :1: SD) plotted relative to US. reference data (Frisancho, 1990). Horizontal bars are :1: so for age. Solid lines represent loth, 50th, and90thpercentiles .......................... 221 FIGURE 4.43 Estimated arm muscle area of EA females (0) 20-75 years al'ld ten-year age group means (A :t SD) plotted relative to US. reference data (Frisancho, 1990). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and90thpercentiles .......................... 222 xviii FIGURE 4.44 Estimated arm muscle area of FN females (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to US. reference data (Frisancho, 1990). Horizontal bars are :1; SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 223 FIGURE 4.45 BMI of EA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data . (Najjar and Rowland, 1987). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 224 FIGURE 4.46 BMI of FN males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Najjar and Rowland, 1987). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 225 FIGURE 4.47 BMI of EA females (0) 5-19 years and five-year age group mm (A :1: SD) plotted relative to US. reference data (Najjar and Rowland, 1987). Horizontal bars are i SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 226 FIGURE 4.48 BMI of FN females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Najjar and Rowland, 1987). Horizontal bars are i SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 227 FIGURE 4.49 BMI of EA males (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to US. reference data (Najjar and Rowland, 1987). Horizontal bars are 1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 228 FIGURE 4.50 BMI of FN males (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to US. reference data (N ajjar and Rowland, 1987). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 229 FIGURE 4.51 BMI of EA females (0) 20-75 years and ten-year age group means (A 1 SD) plotted relative to US. reference data ajjar and Rowland, 1987). Horizontal bars are 1 SD for age. (N S<>lid lines represent 10th, 50th, and 90th percentiles .......................... 230 IFIGURE 4.52 BMI of FN females (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to US. reference data ajjar and Rowland, 1987). Horizontal bars are :1: SD for age. (N Solid linesrepresent 10th, 50th, and90thpercentiles .......................... 231 FIGURE 4.53 Triceps skinfold of EA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data ajjar and Rowland, 1987). Horizontal bars are :1: SD for age. (N SOlid lines represent 10th, 50th, and 90th percentiles .......................... 232 xix f? M1 '23.— 1 OT ...— m [7 _' r»;- V r-' FIGURE 4.54 Triceps skinfold of FN males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Najjar and Rowland, 1987). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 233 ‘ FIGURE 4.55 Triceps skinfold of EA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Najjar and Rowland, 1987). Horizontal bars are i SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 234 FIGURE 4.56 Triceps skinfold of FN females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to US. reference data (Najjar and Rowland, 1987). Horizontal bars are 1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 235 FIGURE 4.57 Triceps skinfold of EA males (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to US. reference data (Najjar and Rowland, 1987). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 236 FIGURE 4.58 Triceps skinfold of FN males (0) 20-75 years and . ten-year age group means (A :1: SD) plotted relative to US. reference data (Najjar and Rowland, 1987). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 237 FIGURE 4.59 Triceps skinfold of EA females (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to US. reference data (Najjar and Rowland, . 1987). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 238 FIGURE 4.60 Triceps skinfold of FN females (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to US. reference data (Najjar and Rowland, 1987). Horizontal bars are :1: SD for age. S olid lines represent 10th, 50th, and 90th percentiles .......................... 239 FIGURE 4.61 Subscapular skinfold of EA males (0) 5-19 years and five-year age group means (A 1: SD) plotted relative to US. reference ta (Najjar and Rowland, 1987). Horizontal bars are :1: SD for age. da Solid lines represent 10th, 50th, and 90th percentiles .......................... 240 FIGURE 4.62 Subscapular skinfold of FN males (0) 5-19 years and five-year age group means (A i SD) plotted relative to US. reference ta (Najjar and Rowland, 1987). Horizontal bars are :1: SD for age. da Solid lines represent 10th, 50th, and90thpercentiles .......................... 241 1FIGURE 4.63 Subscapular skinfold of EA females (0) 5-19 years a131d five-year age group means (A :1: SD) plotted relative to US. l"tfrference data (Najjar and Rowland, 1987). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles ............... 242 IFIGURE 4.64 Subscapular skinfold of FN females (0) 5-19 years and five—year age group means (A :1: SD) plotted relative to US. reference ta (Najjar and Rowland, 1987). Horizontal bars are :1: SD for age. da SOlid lines represent 10th, 50th, and 90th percentiles .......................... 243 xx FIGURE 4.65 Subscapular skinfold of EA males (0) 20—75 years and ten-year age group means (A :|: SD) plotted relative to U.S. reference data (Najjar and Rowland, 1987). Horizontal bars are i SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 244 FIGURE 4.66 Subscapular skinfold of FN males (0) 20-75 years and ten-year age group means (A :l: SD) plotted relative to U.S. reference data (Najjar and Rowland, 1987). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 245 FIGURE 4.67 Subscapular skinfold of EA females (0) 20—75 years and ten-year age group means (A :l: SD) plotted relative to U.S. reference data (Najjar and Rowland, 1987). Horizontal bars are 1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 246 FIGURE 4.68 Subscapular skinfold of FN females (0) 20—75 years and ten-year age group means (A :l: SD) plotted relative to U.S. reference data (N ajjar and Rowland, 1987 ). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 247 FIGURE 4.69 Combined grip strength (right + left) of EA males (0) 5-19 years and five-year age group means (A i SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 248 FIGURE 4.70 Combined grip strength (right + left) of FN urales (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :l: SD for age. S olid lines represent 10th, 50th, and 90th percentiles .......................... 249 FIGURE 4.71 Combined grip strength (right + left) of EA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :|: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 250 FIGURE 4.72 Combined grip strength (right + left) of FN females (0) 5-19 years and five-year age group means (A :l: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). orizontal bars are 1 SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 251 FIGURE 4.73 Combined grip strength (right + left) of EA lbales (0) 20-75 years and ten-year age group means (A :l: SD) plotted r‘elative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 252 FIGURE 4.74 Combined grip strength (right 4» left) of FN males (0) 20-75 years and ten-year age group means (A i SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are :l: SD for age. Solid lines represent 10th. 50th, and 90th percentiles .......................... 253 FIGURE 4.75 Combined grip strength (right + left) of EA females (0) 20-75 years and ten-year age group means (A :l: SD) plotted relative to Canadian reference data (Fitness Canada, 1986). .Horizontal bars are 1 SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 254 FIGURE 4.76 Combined grip strength (right + left) of FN females (0) 20-75 years and ten-year age group means (A i SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 255 FIGURE 4.77 Trunk flexibility of EA males (0) 5-19 years and five-year age group means (A :l: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are 1 SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 256 FIGURE 4.78 Trunk flexibility of FN males (0) 5-19 years and five-year age group means (A :l: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 257 FIGURE 4.79 Trunk flexibility of EA females (0) 5-19 years and five-year age group means (A :l: SD) plotted relative to Canadian x‘teference data (Fitness Canada, 1985). Horizontal bars are 2!: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 258 FIGURE 4.80 Trunk flexibility of FN females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Canadian I‘cference data (Fitness Canada, 1985). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 259 FIGURE 4.81 Trunk flexibility of EA males (0) 20-75 years and ten-year age group means (A :l: SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 260 FIGURE 4.82 Trunk flexibility of FN males (0) 20-75 years and ten-year age group means (A 1 SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 261 FIGURE 4.83 Trunk flexibility of EA females (0) 20-75 years and ten-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 262 xxii FIGURE 4.84 Trunk flexibility of EA males (0) 20-75 years and ten-year age group means (A i SD) plotted relative to Canadian reference data (Fitness Canada, 1986). Horizontal bars are i SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 263 FIGURE 4.85 Sit-ups in BA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles ............... -. .......... 264 FIGURE 4.86 Sit-ups in FN males (0) 5-19 years and five-year age group means (A i SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 265 FIGURE 4.87 Sit-ups in BA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 266 l‘IGURE 4.88 Sit-ups in FN females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 267 FIGURE 4.89 Flexed arm hang in EA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Notor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. . . . 268 FIGURE 4.90 Flexed arm hang in FN males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University ‘ Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. . . . 269 FIGURE 4.91 Flexed arm hang in EA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are 1 SD for age. Solid lines represent 10th, 50th, and 90th percentiles. . . . 270 FIGURE 4.92 Flexed arm hang in FN females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study ~data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. . . . 271 FIGURE 4.93 35-meter dash speed in BA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). orizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. . . .‘ ...................... 272 xxiii iflll‘i FIGURE 4.94 ,35-meter dash speed in FN males (0) 5-19 years and five-year age group means (A i SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are i SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 273 FIGURE 4.95 35-meter dash speed in BA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :t SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 274 FIGURE 4.96 35-meter dash speed in FN females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 275 FIGURE 4.97 Standing long jump in BA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 276 FIGURE 4.98 Standing long jump in FN males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 277 FIGURE 4.99 Standing long jump in BA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 278 FIGURE 4.100 Standing long jump in FN females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are 3: SD for age. Solid lines represent 10th, 50th, and 90th percentiles .......................... 279 FIGURE 5.1 Age-specific TER means for males 5-19 years (A EA, A FN) plotted relative to the median ofthe Quebec Family Study ............................................................... 280 FIGURE 5.2 Age-specific TER means for females 5-19 years (A EA. A FN) plotted relative to the mdian ofthe Quebec Family Study ............................................................... 281 FIGURE 5.3 Ten-year age group TER means for males 20-75 years (4 EA, A FN) plotted relative to the median of the Quebec Family Study ............................................................... 282 xxiv FIGURE 5.4 Ten-year age group TER means for females 20-75 years (A EA, A FN) plotted relative to the median of the Quebec Family Study ............................................................... 283 FIGURE 5.5 Ten-year age group WHR means for males (A EA, A FN) plotted relative to French data (Tichet et al., 1993). Solid lines represent 5th, 50th and 95th percentiles ............................ 284 FIGURE 5.6 Ten-year age group WHR means for females (A EA, A FN) plotted relative to French data (Tichet et al., 1993). Solid lines represent 5th, 50th and 95th percentiles ............................ 285 FIGURE 5.7 Stature in EA Canadian males 5-19 years from studies ranging from 1953 to 1996 ............................................... 286 FIGURE 5.8 Stature in BA Canadian males 20—75 years from studies ranging from 1953 to 1996 ............................................... 287 FIGURE 5.9 Stature in EA Canadian females 5-19 years from studies ranging from 1953 to 1996 ............................................... 288 FIGURE 5.10 Stature in BA Canadian females 20-75 years from studies ranging from 1953 to 1996 ............................................... 289 FIGURE 5.11 Body mass in BA Canadian males 5-19 years from studies ranging from 1953 to 1996 ............................................... 290 FIGURE 5.12 Body mass in BA Canadian males 20-75 years from studies ranging from 1953 to 1996 ............................................... 291 FIGURE 5.13 Body mass in BA Canadian females 5-19 years from studies ranging from 1953 to 1996 ............................................... 292 FIGURE 5.14 Body mass in BA Canadian females 20-75 years from studies ranging from 1953 to 1996 ............................................... 293 FIGURE 5.15 BMI in BA Canadian males 5-19 years from studies ranging from 1953 to 1996 ........................................................ 294 FIGURE 5.16 BMI in BA Canadian males 20-75 years from studies ranging from 1953 to 1996 ........................................................ 295 FIGURE 5.17 BMI in EA Canadian females 5-19 years from studies ranging from 1953 to 1996 ........................................................ 296 FIGURE 5.18 BMI in BA Canadian females 20-75 years from studies ranging from 1953 to 1996 ........................................................ 297 CHAPTER I INTRODUCTION AND CONCEPTUAL FRAMEWORK Introduction Studies of growth and health-related fitness, by design, are generally comparative. For example, growth status is often compared to reference data collected for a representative sample of the population. Alternatively, two subgroups of the population may be compared to each other in any of several growth and fitness parameters. Aboriginal North Americans have a higher prevalence of obesity, diabetes and gall bladder disease than is observed in the general population. This syndrome of diseases, which falls under the rubric of the "New World Syndrome", is hypothesized to have a significant genetic component, as there is an apparent relationship between degree of Native American admixture and the syndrome (West, 1974; Gardner et al., 1984; Weiss et al., 1984; Szathmary, 1994). Among Aboriginal groups in Ontario, the estimated prevalence rates of diabetes are 6.1% for males and 9.2% for females (Young et al., 1990), which is considerably higher than national rates for Canada which have been reported at 1.7% (Statistics Canada, 1981) and 2.4% (Statistics Canada, 1987). A question which is central to the study of the New World Syndrome is Whether the high prevalence of diseases observed in Native groups is the result of a greater genetic susceptibility, or from a greater exposure to environmental stresses. Also of interest is the role of risk factors in the progression of degenerative diseases such as obesity and diabetes, and genetic and environmental influences on the risk factors. Obesity and the distribution of fat within the body are important risk factors 1 2 for disease (NIH, 1985; Ducimetiere et al., 1986; Ducimetiere and Richard, 1989; Després et al., 1990). Physique is also implicated as being associated with disease risk (Malina et al., 1997). Thus, the cause of high prevalence rates for metabolic diseases in North American Aboriginals is likely multifactorial, and a better understanding of risk factors such as obesity, relative fat distribution and physique may be important in understanding the etiology of the New World Syndrome among these groups. In general, the growth of Native North Americans, and in particular, the Canadian First Nation groups, has not been extensively studied. Studies which have been done to date, with a few exceptions, have focused on the nutrition and health of the adult population, with little emphasis on growth. Since it is realized that diseases such as adult-onset diabetes and obesity have their roots in childhood and adolescence, it seems appropriate that emphasis be placed on studying the growth and health characteristics of Aboriginal children and youth. Thus, a study of growth in body size, physique, and indicators of health-related fitness among Canadians of First Nation and European ancestry may provide valuable insights into the observed prevalence rates of the New World Syndrome. Given the proposed relationship between health-related fitness and health, outlined in Figure 1.1, an understanding of how much variation in health-related fitness is due to genetic and environmental factors is important. Studies concerned with estimating familial resemblance in fitness and motor performance phenotypes are confounded by the interrelationships between body dimensions and fitness. Typically studies have not taken into account the influence of body size on fitness when estimating the impact of familial factors on these traits. A key question which remains to be addressed is: given the relationship between anthropometry and fitness (Malina, 1975, 1994), what is the genetic contribution to health-related fitness once the effects of body size have been controlled? 3 Rationale and Purpose of the Study The rationale for this study is three-fold. The first comes from the specific health concerns of First Nation people (Health and Welfare Canada, 1992). Perhaps the adult-onset diseases associated with the adoption of a westernized diet and lifestyle have their roots in childhood and adolescence, such that differences in body size and morphology (morphological fitness) between First Nation and European Canadians may be evident in this period of life. There are little data available describing relative fat distribution and physique in First Nation Canadians, which limits discussions of disease and risk factor prevention in these groups (Young, 1993). The second rationale for this study stems from indications that secular trends in body size have occurred in Canada over the past 35 years. It is estimated that a national secular trend of about 1 cm/decade for stature has occurred from 1956 to 1986 (Shephard, 1986). Persistence of this secular trend limits interpretations of comparisons with reference data collected in 1970-72 (Health and Welfare Canada, 1980) and in 1981 (Fitness Canada, 1983, 1985, 1986). The third rationale for this study is the need for a better understanding’of the interrelationships among genetics, body morphology, and health-related fitness, as outlined in Figure 1.2. The interrelationships among body morphology and fitness phenotypes will be determined using correlational analyses. The family resemblance of body size and morphology will also be determined using correlation and regression techniques, as will the heritability of indicators of health-related fitness, taking into account body morphology as a covariate. The purpose of this study is thus to (1) compare Canadians of European and First Nation ancestry in terms of body size, physique, and indicators of health-related fitness, (2) evaluate secular trends in body size in Canadians of European and First Nation ancestry, and (3) estimate familial resemblance in body size, physique, and indicators of health-related fitness in Canadians of European and First Nation ancestry. 4 Research Hypotheses The specific research hypotheses are as follows: 1) There are significant differences between Canadians of First Nation (FN) and European ancestry (BA) in body size, physique, and indicators of health-related fitness. la) FN Canadians are heavier and demonstrate greater subcutaneous fatness than EA Canadians throughout childhood into adulthood. 1b) There are significant differences in relative fat distribution and physique between FN and EA Canadians. 1c) There are no differences in stature and other skeletal dimensions between FN and EA Canadians. 2) Secular trends in body size are evident in FN and EA Canadians. 2a) There are significant secular increases in stature, mass and the BMI in FN and EA Canadians. 3) There is significant familial resemblance in body size, physique and indicators of health-related fitness in FN and EA Canadians. 3a) There is significant familial resemblance in body size, physique and indiCators of health-related fitness in FN and EA Canadians. 3b) Estimated heritabilities for strength and flexibility are greater after body morphology is factored into the analyses as a covariate. Limitations of Study The study population is unique in that all participants are from the same small community in northern Ontario. This allows a certain control over environmental factors; however, it also limits the applicability of the results to other population groups. The results of this study carry significance for the residents of Temagami and Bear Island, but caution must be used when extrapolating the results to other communities. Given that regional data are not available from the national Canadian surveys (Health and Welfare Canada, 1980; Fitness Canada, 1983, 1985, 1986) for 5 comparison, the results from this study should not be considered representative of the general Canadian population. This study was conducted over only the summer season, limiting the extension of results to other seasons. Anecdotal references by several subjects indicate that there is seasonal variation in body fatness and strength in this population between the summer and winter months. Subjects indicated that both FN and EA people are less active and may accumulate fat in the winter months; thus, the results of this study should be viewed as status during the summer months only. Data on physical activity and dietary intake were not obtained, which limits interpretations about energy balance and speculations regarding differences in body size and fatness between EA and FN subjects. The assignment of racial affinity was made by the participants themselves, such that concrete biological groupings were not possible. Difficulties arose in assigning hybrids with differing degrees of admixture to a specific group. Thus, results of comparisons between FN and EA subjects should be viewed as conservative, as the inclusion of hybrids may temper the results. A I Significance of Study This study has the potential of providing important information on the covariance among health-related phenotypes and the variation in these phenotypes which can be attributed to familial factors. In addition, the growth characteristics of FN children and youth are described and compared to national reference data, adding to the understanding of Aboriginal growth. Little data are available on relative fat distribution and physique in FN Canadians; thus, this study will provide important information on these characteristics. Although the results of this study may not be generalizable to the rest of Canada, the detailed observations from this region are valuable in and of themselves. CHAPTER II _ REVIEW OF RELATED LITERATURE Introduction This study compares growth and adult morphology among groups of First Nation (FN) and European (EA) ancestry, while flaming the data within a familial design which allows inferences about familial resemblance in parameters of growth and health-related fitness. Thus, the literature review is divided into two sections; the first examines body size, fatness, fat distribution, physique and secular trends among Aboriginal groups with comparisons to the general North American population, and the second reviews the current methodologies and understanding of the familial resemblance in body size and health-related fitness. .. e ‘1‘. 3,,‘. '1':_-~ ‘. \; -‘,‘,‘H- 3,1 Introduction Although there is considerable knowledge about the growth of children in general, there is a lack of data describing the growth characteristics of Native North Americans. Several anthropometric studies have been conducted; however, such studies have more often focused on the health and nutrition of adults rather than on the growth of children. Indeed, the first edition of Eveleth and Tanner's W W (1976) cites data from only four descriptive growth studies on Amerindian groups from North America. The subsequent edition (Eveleth and Tanner, 1990) includes growth data from only three more recent studies among Amerindians. This general lack of growth data represents a lacuna in understanding Amerindian health and variability. 7 The study of the growth of Amerindian children is important from several perspectives. First, growth studies are important to understand the nature of the growth process itself. This most basic rationale suggests that a knowledge of growth and maturation is important for understanding the findings of research within the clinical and scientific communities which work with children. Given the lack of data for Amerindian children, a growth study is important in its own right to provide basic information. Second, there are a disproportionately high number of health problems among adult Native Americans compared to the general population which may perhaps be better explained in the context of growth and maturation. Diabetes, obesity, and gall bladder disease, which are particularly problematic for Amerindian groups (West, 1974; Weiss et al., 1984; Young, 1993), may have their origins in processes occurring during the growth period, beginning prenatally and extending through adolescence. Third, a more theoretical approach to the study of human growth has been the association between patterns of growth and specific environmental influences, such as i residence in cold climates or high altitudes, which may help to explain the origins of human variability (Leonard et al., 1994, 1995). Thus, the study of diverse ethnic groups is important for the formulation of evolutionary theories about human variability. Fourth, the realization that growth parameters can serve as indicators of the quality of the physical environment has led many investigators to consider childhood growth as an important index of health and the standard of living for entire populations (Tanner, 1994). Such a rationale indicates that the health status of the Native American population may be reflected in the growth of children in these groups. This review summarizes the available data on the growth of North American Aboriginals and compares the data to reference data for Canada and the U.S. The rcference data are derived largely from samples of European ancestry. 8 Growth Data The available data on the growth of Aboriginal North Americans are largely limited to health studies in which the main focus is the health and nutritional status of the population. Considerably more data are available on the anthropometry of adult Natives than on children; however, the main focus of this review is the work completed with children and adolescents. Data on adults are presented for the purpose of comparing the end product of growth, i.e., adult morphology. Descriptive growth studies have been conducted among the Navajo (Darby et al., 1956), Apache (Kraus, 1961), Gros Ventres and Assiniboin (ICNND, 1964b), Blackfeet (ICNND, 1964a), Nootka and Chilcotin (Birkbeck et al., 1971), Athapaskan (Lee and Birkbeck, 1977), Chippewa, Sioux and Winnebago (Johnston et a1. 1978), and Cree (Coodin et al., 1980). The Alaskan Eskimos are among the most studied of the North American groups, and several studies have been published characterizing their growth (Heller et al., 1967; Jamison, 1970; Johnston et al., 1982; Rode and Shephard, 1994). Body Size W Growth studies among Native North Americans generally demonstrate few differences in stature and body mass between children of Aboriginal and European ancestry. Comparisons of stature and body mass between the Navajo and a nationally represented Canadian sample revealed no significant differences between the groups (Darby et al., 1956). Similarly, a comparison of growth rates among the Apache, African Negroes, and American Whites (Irish ancestry) found no differences based on regression lines (Kraus, 1961). A study among Cherokee adolescents demonstrated that mean statures of youths 13-17 years were similar to National reference data; however, mean body masses were significantly greater than the reference data in both boys and girls (Story et al., 1986). 9 The United States Interdepartmental Committee on Nutrition for National Defense (ICNND, 1964a; 1964b) conducted nutrition surveys on the Indian reservations at Fort Belknap, Montana (Gros Ventres and Assiniboin) and the Blackfeet Reservation located to the west of Fort Belknap (Moore, 1972). Statures and body masses of children 6-11 years of age were compared to Iowa reference data. All of the children in both studies tended to be at or above the reference mdians in stature and body mass. The Gros Ventres/Assiniboin boys tended to deviate the most in body mass fiom the reference children. In a study of urbanization of Chippewa, Sioux, and Winnebago in Minneapolis, Johnston et al. (1978) noted that Native children were similar in stature to U.S. reference values until 12 years of age in boys, when the native sample began to lag behind. By 17 years of age, the reference mean was 6 cm taller than the Minneapolis Native boys samme. Body masses, however, showed a clear and consistent difference, with the Native boys and girls being heavier than the reference means beginning with age 2. Data from Canadian Aboriginal groups parallel those fiom studies conducted in the United States. Coodin et al. (1980) reported that both the stature and body mass of Northern Manitoba Cree from birth to 6 years of age were similar to those reported for U.S. children; however, weight-for-height of the girls exceeded the reference. Studies among Athapaskan Aboriginals in British Columbia and the Yukon Territory indicated that they were shorter than Iowa reference values; however, all of the children were within 2 standard deviations of the reference (Birkbeck et al., 1971; Lee and Birkbeck, 1977). In some samples there was a tendency for the statures and body masses to lag, relative to the Iowa reference during adolescence (Lee and Birkbeck, 1977). The authors suggested that the results were consistent with moderate growth retardation during late adolescence; however, the biochemical data failed to reveal any signs of nutrient deficiencies. 10 Eskimo children are consistently shorter than other Aboriginal groups and nationally representative reference data. Heller et al. (1967) reported that the mean stature of the Eskimo children followed the 5th percentiles for White children until 16 years of age. Mass, however, was as great as that for White children until about 6 years of age; thereafter, Eskimo children weighed less. Similarly, St. Lawrence Island Eskimo children were shorter than both White and Black U.S. reference means, but body mass in the Eskimos was similar to U.S. reference values (Johnston et al., 1982). Jamison’s (1970) analysis of the growth characteristics of a sample of Wainwright Eskimos indicated that adults were, on average, 12 cm shorter than North American Whites, and that they did not reach final adult height until they were in their mid- twenties. The distribution of statures among Native North Arrrerican groups generally overlaps those of the general Canadian population. Figures 2.1 and 2.2 illustrate growth in stature for Native American boys and girls, respectively, from age 4 to 18 years. The solid curves represent the 10th and 90th percentiles of Canadian children in the 1970s (Health and Welfare Canada, 1980). In both boys and girls, age-specific means fall between the 10th and 90th percentiles. Eskimos from Alaska (Heller et al., 1967) and specifically from Wainwright (Jamison, 1970) are among the shortest of the native groups; the Alaskan Eskimos fall below the 10th percentile in the older age groups in both boys and girls. The Gros Ventres/Assiniboin from Fort Belknap (ICNND, 1964b) are among the tallest of the groups depicted in the figures between the ages of 6 and 10 (the age range for that study), tracking between the 50th and 75th Percentiles for both boys and girls (note: the 50th and 75th percentiles are not shown). As with stature, there is considerable overlap in the mean body masses of Native groups. Figures 2.3 and 2.4 illustrate growth in body mass from 4 to 18 years in Amerindian boys and girls, respectively. The solid curves represent the 10th and 90th percentiles of Canadian children in the 19705 (Health and Welfare Canada. 1980). 11 All age-specific means fall between the 10th and 90th percentiles. The Gros Ventres/Assiniboin (ICNND, 1964b) and the Chippewa, Sioux and Winnebago (Johnston et al., 1978) are among the heaviest girls and boys in the older age groups. Among males, the Apache and Navajo are among the lightest groups at younger ages, whereas Alaskan Eskimos are the lightest at older ages. In girls, Alaskan Eskimos are the lightest group throughout the age range represented. mummies! Considerable diversity in adult stature and body mass exists among selected Amerindian groups (Table 2.1). It is difficult to compare adult values to a nationally representative sample of Canadians (or to each other), as the age ranges vary and possible secular variation is not controlled (Jamison, 1970; Miller, 1970; Sugarrnan et al., 1990: Knowler et al., 1991; Rode and Shephard, 1994). Blackfeet men and women appear to be tallest, while the Eskimos are shortest. This difference appears to be quite real, as it is unlikely that a secular trend over one generation could have accounted for the observed difference of 11.1 cm in men and 9 cm in women between these two groups. The Eskimo women measured by Jamison and Zegura (1970) in Wainwright, Alaska are the heaviest group; however, they are also the shortest. The heaviest men in are the Chilcotins from British Columbia, while the lightest are the Alaskan Eskimo men. Fatness and Relative Fat Distribution Fatness ' Body fatness is generally estimated in anthropological studies fiom skinfolds or the BMI. The former indicates subcutaneous adipose tissue, while the latter is an indirect indicator of obesity. In the general adult population, the BMI is correlated with fatness (Roche et al., 1981) and is independent of stature, but its utility in children is limited since it is correlated with stature (68111 et al., 1986). However, the BMI is commonly used in children as it is simple to calculate from stature and body mass. 12 WW There are little data available on the BMI and subcutaneous fatness of Amerindian children and youth. Johnston et al. (1978) reported that among Minneapolis school children 6-17 years, urban Natives had greater weight-for-height indices than national reference data Among Navajo schoolchildren 5-17 years, mean BMIs also exceeded national reference data (Sugannan et al., 1990). Similarly, Story et a1. (1986) indicated that in Cherokee youth 13-17 years, mean BMIs were consistently greater than reference values in all age and sex groups. Estimated BMIs were calculated from the stature and mass means presented in the previous section. Figures 2.5 and 2.6 present estimated BMIs for boys and girls, respectively, for ages 4 to 18 years. Solid lines represent the 5th and 95th percentiles for the BNII in U.S. children (Must et al., 1991). All age-specific estimates for the BMI fall between the 5th and 95th percentiles in both boys and girls. There is considerable overlap in the distributions among groups in both sexes. It is thus difficult to determine population differences based on these estimates. The prevalence of obesity among Amerindian children and youth varies by geographic location and tribal affiliation. Malina (1993) examined prevalences of obesity in North American children and youth (defined as 285th percentile NHANES II BMI) fi’om 11% in Chippewa females to 78.3% in Southwestern Arizona Native females. There was also a trend over time, with older samples having lower estimated prevalences of obesity than more recent samples. These results are similar to those presented by Broussard et al. (1991), which indicated that the estimated prevalence of obesity among Native American schoolchildren varies considerably by region. The review demonstrated that the average prevalence of overweight among Native American adolescents was 24.5% in males and 25.0% in females. Compared to data from the Ten State Nutrition Study, prevalence rates of obesity in Cherokee youth (285th 13 percentile triceps skinfold) were 49.7% in boys and 31.6% in girls 13-17 years (Story et al., 1986). In a review of anthropometric variation among Amerindian groups, Johnston and Schell (1979) indicated that, in general, mean skinfold thicknesses tend to vary as does body mass. Compared to U.S. reference data, mean subscapular skinfold thicknesses in Chippewa males and females from infancy through 18 years of age were significantly greater than the reference medians, whereas in males, triceps skinfold thicknesses were significantly less than the reference medians below 6 years of age; thereafter, they were significantly greater than the reference medians (Johnston and Schell, 1979). Similarly, triceps skinfold thicknesses of Manitoba First Nation school children were well within the distribution for the general U.S. population (Coodin et al., 1980). Alternatively, Story et al. (1986) presented evidence that Cherokee youth demonstrated significantly greater means and medians for the triceps skinfold compared to U.S. reference data, indicating that these youngsters were somewhat fatter than the average American child WW There are more data on the BMI of adult Aboriginal groups than for children. Among selected samples of North American Aboriginals, Eskimo women have the highest BMI, while Nootka men are the heaviest for stature (Table 2.1). Although variable, there does not appear to be a clear pattern of variation in the BMI by geographic location. There is consistent evidence that the prevalence of obesity, defined by the BMI, is greater in Aboriginal groups than the general population. In a survey of U.S. Native groups, Broussard et al. (1991) estimated prevalences of overweight (BMI 2 27.8 in males and BMI 2 27.3 in females) in adults 2 18 years at 33.7% in males and 40.3% in females, which were higher than national estimates for the U.S., 24.1% and 25% in males and females, respectively. Age specific prevalences of overweight (BMI 2 27.8 14 in males and BMI 2 27.3 in females) among the Pima ranged from 31% to 78% for males 220 years, and fiom 48% to 87% for females 2 20 years (Knowler et al., 1991). Navajo adults were also significantly overweight (Hall et al., 1991). The prevalence of overweight (BMI 2 27.8 in males and BMI 2 27.3 in females) was 30.3% in males and 50.0% in females. Prevalence data for Canadian First Nation groups parallel those from the United States. The proportion of a Northwestern Ontario population of Cree and Ojibwa classified as obese (BMI 2 27 in males and BMI 2 25 in females), increased with age; 70% of women 35 to 64 years and 50% of men 35 to 44 years were obese (McIntyre and Shah, 1986). Similarly, Gittelsohn et al. (1996) estimated prevalences of obesity fiom 22.8 % to 50.0% in Ojibwa and Cree from Northwestern Ontario (BMI 2 30); and prevalences also increased with age. Young and Sevenhuysen (1989) surveyed four Northern Canadian First Nation conununities. The data suggested that the prevalence of obesity was higher in Native groups compared to Canadian national averages; however, specific figures were not indicated. Relative Fat Distribution Relative fat distribution refers to the relative amount of fatness, subcutaneous or visceral, in different regions of the body (Malina, 1996). Two common indicators of relative fat distribution are the ratio of trunk/extremity skinfolds (TER) and the waist/hip circumference ratio (WHR). The TER is relatively simple and is useful when measuring large numbers of people; however, its use assumes that trunk and extremity subcutaneous fatness increase in a linear manner (Garn et al., 1982). The WHR is useful in adults, but its utility in children and youth has not been established (Malina, 1 996). W A single study considered relative fat distribution among Native North American children (Johnston et al., 197 8). Among urban Native schoolchildren, 15 triceps skinfolds were below reference medians for the first 5 years of life in males: thereafter, they were larger than the reference values. In contrast, subscapular skinfolds were consistently larger than the reference medians. In females of all ages, triceps skinfolds were consistently below, whereas subscapular skinfolds were consistently above reference medians. The data thus suggest that Native children accumulate proportionally more subcutaneous fat on the trunk than the extremities. Note, however, inference on relative subcutaneous fat distribution based on only two skinfolds must be made with caution (Malina, 1996). was! There are little data available on relative fat distribution among Native North Americans. Young and Sevenhuysen (1989) indicated that the obesity among four Northern Canadian First Nation groups was primarily of the central type as gauged by the subscapular/triceps skinfold ratio and the WHR. Although raw data were not presented, the authors indicated that 36% of men and 11% of women had WHRs >0.99, which corresponds to approximately the 95th and 99th percentiles of French reference data for men and women, respectively (Tichet et al., 1993). Also, 29.8% of men and 22.4% of women had subscapular/triceps ratios >1.64. Among Canadian Eskimos, a truncal fat distribution (ratio of triceps/subscapular+suprailiac skinfolds) skinfolds was greater in women than men (Schaefer, 1977). A similar observation in women has been made in other cold adapted populations such as the Evenki reindeer herders of the central Siberian taiga (Leonard et al., 1994) and Mongolian pastoralists (Beall and Goldstein, 1992). It has been hypothesized that a central distribution of subcutaneous fat is an adaptation to the cold. Caution must be used when interpreting these results, however, as relative subcutaneous fat distribution is fat dependent. In other words, as overall fatness increases, the ratio of trunk/extremity fatness also increases (Garn et al., 1982; Malina, 1996). On the other hand, the subscapular/triceps 16 ratio among Canadian Inuit and Siberian nGanasan 17-49 years of age was higher in men than women (Rode and Shephard, 1995). There is an apparent relationship between a truncal fat distribution and adult- onset diabetes (NIDDM) among Native groups. Among female Navajo, risk of NIDDM increased with an increase in the WHR. A similar nonsignificant trend was evident in the males. Mean WHRs were 0.96 for males 20 years and 0.90 for females 2 20 years. These values correSpond to approximately the 75th and 95th percentiles of French reference data for males and females, respectively (Tichet et al., 1993). Szathmary and Holt ( 1983) used principal components analysis and hierarchical analysis of variance to examine the association between relative fat distribution and blood glucose levels among the Dogrib in the Northwest Territories. Although the analysis did not provide a measurable fat distribution phenotype, there was a significant association between truncal fat distribution (as assessed by principal components of subscapular, midaxillary, suprailiac, abdominal, triceps, forearm, and medial calf skinfold sites) and elevated blood glucose levels (Szathmary and Holt, 1983). Physique Physique refers to the configuration of the body as a whole. It is most often quantified as a somatotype, which characterizes physique or body build in three components: endomorphy, mesomorphy, and ectomorphy. Currently the Heath-Carter anthropometric protocol for estimating somatotype is most widely used (Carter and Heath, 1990). There are very little data on the somatotypes of Native North Americans. A single study among Wainwright Alaska Eskimos 16-75 years of age, measured by Jamison and Zegura in 1958, using both the Heath-Carter anthropometric and photoscopic techniques (ratings by Heath) indicated that Eskimo men and women had a physique characterized by high endomorphy and mesomorphy, with many extreme mesomorphs (Carter and Heath, 1990). l7 Secular Trends Secular trends in stature, body mass and fatness have been examined in Native North American populations (Jamison, 1970; Miller, 1970; Knowler et al., 1981, . 1991; Sugarman et al., 1990; Rode and Shephard, 1994). Miller (1970) demonstrated a 1.3 cm increase in stature and a 5.9 kg increase in body mass between 1940 and 1967 (one generation) of Apache men; change per decade was not estimated. The change in stature was similar to the secular change which was occurring in military recruits over the same time frame; however, the secular change in body mass was quite dramatic. The secular change in body mass was attributed to shifts in dietary patterns as the Apache adopted a "westernized" diet with acculturation into American society. This dietary shift, in combination with a susceptible genotype, has been used to explain the increased incidence of diabetes and obesity in Native groups (W eiss et al., 1984). The Pima Indians of Arizona are obese, on average, compared to national reference data for the United States (Knowler et al., 1991). Additionally, there has been a modest increase in the BMI among adult Pima over the last 25 years. In almost all age and sex groups, the mean BMI increased in the sampling periods 1965-72 to 1973-80 to 1981-88. The mean BMI of the youngest adult age groups were also higher than that of the older age groups (Knowler et al., 1991). Pima children 5 through 18 years may have undergone a secular trend in body mass since the turn of the century. Weights of Pima children, adjusted for height by linear regression, were 6 kg heavier than children of comparable height in measured in 1908 (Knowler et al., 1981). Navajo schoolchildren were taller and heavier in 1989 than they were in 1955 (Sugarman et al., 1990). Compared to the data collected in 1955 (Darby et al., 1956), Navajo boys and girls in 1989 were 6.1% and 4.4% taller, respectively. Similarly, mean weights increased 28.8% and 18.7% in boys and girls respectively, from 1955 to 1989. No estimate of change per decade was given. The authors speculated that the 18 secular changes were the result of changes in nutrition, daily energy expenditure, and the availability of health care. Eskimos of Wainwright, Alaska, and Northern Canada have also experienced secular changes in stature and body mass. Jamison (1970) noted that Wainwright Eskimos in 1969 were taller then those of 1955, and by using a regression approach on the birthdates, an increase of 4-6 cm between the years 1880 and 1940 was estimated. A secular trend towards earlier maturation may have occurred among the Canadian Inuit, and this influences stature. Rode and Shephard (1994) published an update of their ongoing work with the Canadian Inuit of Igloolik in the Northwest Territories. The data suggested that at younger ages (10- 12 years), children were taller in 1990 than in 1970 (+1.7%); however, in the older ages (17-19 years), there was a trend for shorter stature in 1990 than observed in 1970 (-2.2%). The trends were the same in both males and females. Estimates were a 1.1 cm/decade increase in the youngest students, and a -1.7 cm/decade decrease in 17-19 year olds. The authors suggested that the trend may be due to earlier maturation, but there are little data on which to base this assumption. An alternative hypothesis put forth by the authors was that high speed snowmobile driving causes vertebral compression in the older children, resulting in relatively shorter individuals in the older age groups. This hypothesis, however, has not been tested. The data showed no consistent “trends in body mass over the three decades, but the sum of three skinfolds indicated that the 1990 sample was significantly fatter than previous samples from 1970 and 1980 (Rode and Shephard, 1994). Since few studies of Amerindians provide estimates of change per decade, it is difficult to determine if secular changes are comparable to those in the general population. Positive secular trends have occurred in all socioeconomic groups in Europe, Japan and the United States (Malina, 1990). Tanner (1988) suggested that between 1880 and 1950, a secular change of approximately 1 cm/decade in adults had 19 occurred in Europe and the U.S. Given the ever-changing demographic composition of the U.S., it is difficult to estimate secular trends; however, data from several national surveys in the U.S. in the 1960’s and 1970’s indicate that the trend towards larger body size has ceased (Malina, 1990). Additionally, there is no consistent evidence for differences in secular increases between Black and White children. Comparisons of the BMI in Americans between 1960 and 1980 indicated no change in the BMI in males 18- 34 years, but both Black and White females showed an increase over this span (Malina, 1990). Summary The data suggest that among Aboriginal groups, adult Eskimos are shortest in stature, while other groups such as the Gros Ventres/Assiniboin and Chippewa, Sioux and Winnebago are tallest. There are no clear trends in body mass; however, Eskimo women, as well as being the shortest, are also the heaviest. The prevalence of obesity among Amerindian groups varies by geographic location and tribal affiliation. There is consistent evidence that the prevalence of obesity, defined by the BMI, is greater in Aboriginal groups than in the general North American population. Data on relative fat distribution among Native groups are limited, and results indicate that North American Aboriginals, particularly adults, have a more- central or truncal fat distribution. Data are equivocal regarding sex differences in fat distribution. Secular trends appear to have occurred in Native North Americans. The Pima are among the most obese of the Native groups, and the mean BMI appears to have increased over the last 25 years. A single study of secular change in Inuit children suggests that earlier maturation may be accounting for taller children at younger ages and shorter older children in 1990 than in 1970 within the same community. Estimated rates of change are rarely provided, which would aid in comparing studies. Introduction There is considerable interest in determining the familial resemblance in body size and components of health-related fitness. Fitness is generally defined as an individual’s ability to withstand stress, and is usually defined in terms of health- and performance-related components (Caspersen et al., 1985). Performance-related fitness refers to the individual’s ability to perform physical work optimally, whereas health- related fitness refers to those phenotypes which relate to health status. The definition of health-related fitness has recently been expanded to include five components (Table 2.2): morphological, muscular, motor, cardiorespiratory, and metabolic (Bouchard and Shephard, 1994). This section outlines the types of studies which have been used to estimate the heritability of phenotypic characteristics as well as the evidence thus far accumulated to suggest a familial component in the observed variability in body size and health-related fitness. For the purpose of this review, morphological fitness phenotypes, such as the BMI, fatness, and relative fat distribution, will be considered in the section on fatness and relative fat distribution. Types of Studies Two major types of studies have been used to estimate the contribution of genetic factors to phenotypic variation. The first design is based on studying populations, while the second is based on studies of related individuals. Population studies generally attempt to estimate the influence of genetic and environmental factors for a given characteristic (Bouchard and Malina, 1983). Anthropological studies of populations generally compare populations of similar ancestry living under different environmental conditions, or alternatively, compare populations of different ancestry living under similar environmental conditions 21 (Bouchard and Malina, 1983). Population studies are typically concerned with determining the extent to which genetic and environmental influences have impacted given characteristics within a given population; in other words, these are studies of adaptation and accommodation (Frisancho, 1993). Studies of relatives have a long tradition in population genetic research. Studies of this type generally consider phenotypic variation among relatives. Basic designs may consider only relationships among nuclear family members, whereas other designs may involve extenkd pedigrees and complex statistical analyses aimed at investigating genetic mechanisms associated with a given trait, or genetic associations among traits (Bouchard and Malina, 1983; Rice et al., 1995). Studies of relatives can generally be divided into two main types, family and twin. Family studies usually involve examining associations among all possible relatives, generally through the use of spouse, sibling, and parent-offspring correlations. More complex studies may include relationships among extended relatives (aunts, uncles, cousins, etc.). Examples of large family studies include the Quebec Family Study (QFS, Bouchard et al., 1988), the Canada Fitness Survey (CFS, Perusse et al., 1988), the Framingham Offspring Study (FOS, Heller et al., 1984), the Muscatine Ponderosity Family Study (MPFS, Moll etal., 1991), and the Nord- Trandelag Norwegian National Health Screening Service Family Study (Tambs et al., 199 l ). Study of adopted relatives is often useful in determining the influence of genetic and environmental factors on a particular trait. Examples of adoption studies include those utilizing the Montreal Adoption Survey (Annest et al., 1983; Biron et al., 1977) and the Danish Adoption Register (Sorensen et al., 1992a, 1992b). Twin studies are specific investigations which eXamine variation within and among pairs of monozygotic (MZ) and dizygotic (DZ) twins. Although relatively simple in design, the twin method is more complex than originally thought (Bouchard 22 and Malina, 1983). Significant biases may result from (1) differences in means of twin types, and (2) associations between twin type and the phenotypic variance of the trait of interest (Bouchard and Malina, 1983). Twin studies generally provide higher estimates of heritability for a given phenotype than family studies, which should be taken into account when interpreting results from different studies. Twin studies typically present associational data in the form of intrapair correlations for various phenotypes such as fatness, physical working capacity, and strength (Komi et al., 1973; Engstrbm and Fischbein, 1977; Price et al., 1987). Twin studies have also been used to study genotype-environment interactions, especially with regard to changes in morphology following training and/or dietary manipulation (Poehlman et al., 1986, 1987; Bouchard et al., 1990). Examples of large twin studies include the Leuven Longitudinal Twin Study (Macs et al., 1993, 1996) and studies utilizing the Danish Twin Register (Herskind et al., 1996), the Finnish Twin Cohort (Korkeila et al., 1991), and the Swedish Twin Registry (Stunkard et al., 1990). Body Size This section focuses on familial resemblance in stature and body mass. In a review of 24 studies presenting parent-child correlations in stature and body mass, Mueller (1976) indicated that the heritability of stature in school aged children from 6 studies varied from 0.31 to 0.58 based on mid-parent regressions. Heritability approximations based on twice the parent-child correlation in the same 6 studies yielded higher estimates ranging from 0.44 to 0.88. However, the average parent-child combined correlation for stature from 20 studies demonstrated a heritability of 0.62. Body mass, based on parent child correlations from 9 studies had an average heritability of 0.52 (Mueller, 1976). There is considerable variability in estimates of the familial resemblance in stature and body mass among family studies conducted since this early review in 1976 (Tables 2.3 and 2.4). Spousal correlations should theoretically approximate zero in the 23 absence of assortative mating, since spouses are biologically unrelated. However, spousal correlations for stature range from 0.06 in U.S. Blacks (Malina et al., 1976) to 0.43 in the CFS (Pérusse et al., 1988). Similarly, spousal correlations for body mass range from 0.15 in a sample of African Americans (Rotimi and. Cooper, 1995) to 0.39 in a sample of Canadians fiom Montreal (Biron et al., 1977). These results indicate that assortative mating for stature and body mass occurred in some groups. In addition, shared lifestyles contribute to the spousal similarities in body mass (Ramirez, 1993). Sibling and parent-offspring correlations indicate significant familial aggregation of stature and body mass (Tables 2.3 and 2.4). For stature, sibling correlations range from 0.14 to 0.67, whereas parent-offspring correlations range from 0.01 to 0.67. Similarly, sibling correlations for body mass range from 0.16 to 0.61 and parent-offspring correlations range from 0.16 to 0.52. Within studies, spousal correlations, with few exceptions, are generally lower than those for first-degree relatives, indicating that genetic factors may be contributing to the covariation among families. However, given that spousal correlations reach the strength of the association among first degree relatives in some cases, common environmental (household) effects may also be important. It is difficult to infer genetic effects from familial correlations, since the effects of genetic factors cannot be distinguished from common environmental factors due to cohabitation. The purpose of most of the studies surveyed in Tables 2.3 and 2.4 was to examine familial resemblance in stature and body mass; however, three of the studies used additional analyses to estimate genetic and environmental contributions to stature and body mass. Perusse et al. (1988), using path analysis (TAU model), determined that transmissibility fiom parent to offspring (genetic and cultural) accounted for 27% and 28% of the variability in stature and body mass, respectively. Thus, non-transmissible 24 factors such as shared sibling environment, personal lifestyle, and variation due to unreliability of measurements accounted for over 70% of the variance in stature and body mass in this sample. The role of the shared environment may not be the same for both stature and mass. Using maximum likelihood methods, Annest et al. (1983) determined that genetic factors made a highly significant contribution to the familial aggregation of both stature and mass, whereas common household effects contributed significantly to variation in stature, but not in body mass. A study of adopted and natural children and their parents indicated a significant genetic component of the variability in body mass (Biron et al., 1977). The correlation between natural parents and their children was significant (r=0.31, p<.001); however, the correlation between adoptive children and adoptive parents was not significant (r=0.01, p=.85). Similarly, the correlation among natural siblings was significant (1:039, p<.001) and the correlation among adoptive children was not significant (r=0.01, p=.94). Circumferences and Skeletal Dimensions Circumferences Circumferences are heterogeneous measurements (Bouchard et al., 1997). A given circumference includes skin, adipose tissue, and various underlying tissues depending on the location of the measurement. Although simple to measure, data on the family resemblance in body circumferences are very limited. Evidence indicates a significant familial component in the variance of several body circumferences (Table 2.5). Spousal correlations range from 0.04 to 0.31, indicating assortative mating in some groups. Sibling correlations for body circumferences are generally higher than parent-offspring correlations within studies, suggesting that shared environment is important in explaining variation in these traits. Sibling correlations range from 0.09 to 0.50 and parent-offspring correlations range from 0.14 to 0.53. In general, correlations are of similar strength among studies and 25 across all circumferences. Family correlations vary with the age and sex of the subjects. Byard et al. (1983) indicated that sibling correlations for calf circumference varied with the age of the siblings, and that correlations decreased at 12-13 years of age in boys and at 11-12 years in girls. The decreases corresponded to periods of variable growth rates around the pubescent growth spurt in stature. The strength of correlations was also different when children and adult family members were compared. Skeletal Dimensions Familial resemblance in skeletal dimensions is supported by data from several studies (Table 2.6). Surprisingly, few studies report spousal correlations for skeletal dimensions, which makes it difficult to interpret sibling and parent-child correlations in explaining genetic and environmental influences. Sibling correlations range from -0.02 to 0.68, while parent—offspring correlations range from 0.07 to 0.49. Sibling and parent-offspring correlations are generally of similar magnitude within and among studies. Few studies have attempted to estimate the genetic component of the variability in skeletal dimensions. Evidence indicates a genetic component to the variance in skeletal breadths (Clark, 1956; Vandenburg, 1962) as well as genetic pleiotropism such that genetic factors may work synergistically to influence the breadth and robusticity of the skeleton (Bouchard and Lortie, 1984). Note that many anthropometric skeletal dimensions are composites across several bones, e.g., sitting height, biacromial and bicristal breadths, etc. (Bouchard et al., 1997). Bouchard et al. (1980b) estimated heritabilities of 0.48 for sitting height, 0.62 for biacromial breadth, 0.22 for bicristal breadth, 0.50 for bicondylar breadth and 0.54 for biepicondylar breadth. These estimates were computed as two times the sibling correlation, controlling for seven socioeconomic familial indicators. Using twin data, Kramer et al. (1986) estimated the heritability of bicristal breadth as 0.51, which was higher than the estimate of Bouchard 26 et al. (1980b); however, twin studies generally generate higher heritability estimates than family studies. Fatness and Relative Fat Distribution The genetics of body fat and relative fat distribution have recently become of great concern, mostly because of the identification of obesity as an important risk factor for many diseases. Variables such as the BMI, percentage body fat, skinfolds, and trunk/extremity skinfold ratios have been utilized in several studies in an attempt to quantify the genetic contribution to fatness, obesity and relative fat distribution. BMI The most frequently studied index of obesity is the BMI (mass/stature), although the BMI is actually a measure of heaviness, as it does not distinguish between lean and fat tissues. Any estimate of the genotypic variance of the BMI will thus be . contaminated by the unknown genotypic effects on both fat and fat-free tissues (Bouchard, 1989). There is considerable variability in estimates of familial resemblance in the BMI (Table 2.7). Spousal correlations indicate a small assortative mating effect, especially in an Indian sample (r==0.37, Nirmala et al., 1993). With this exception, spousal correlations for the BMI range from -0.05 to 0.19. The range of estimates probably represents different mating strategies among world populations and different contributions of the home environment to the BMI in different ethnic groups. Correlations among first-degree relatives for the BMI are consistently higher than spousal correlations. Parent-offspring correlations range from 0.02 to 0.38; however, with the exception of two studies (Ramirez, 1993; Annest et al., 1983), estimates of heritability based on two times the parent-offspring correlation range from 0.36 to 0.76. This agrees well with the overview of Bouchard et al. (1997), suggesting that the heritability of the BMI derived from family studies is generally about 0.30 to 0.50. Further, twin studies tend to produce higher estimates of 27 heritability, while adoption studies yield lower estimates. Combining research strategies, the estimated heritability of the BMI is approximately 0.25 to 0.40 (Bouchard et al., 1997). Body Fat Studies investigating the genetics of direct assessments of body fatness are few (Bouchard and Perusse, 1988). Ramirez (1993) estimated lean body mass using bioelectrical impedance and in turn percentage body fat. Sibling correlations were 0.21 (brother-brother) and 0. 36 (sister-sister), and parent—offspring correlations ranged from 0.17 to 0.25, after adjusting for the effects of age and the BMI. Bouchard et al. (1988) considered percentage and total body fat estimated from densitometry in 1,698 relatives from the Quebec Family Study. Interclass correlations were 0.23 for parent-offspring comparisons and 0.17 in siblings for percentage body fat. The results were consistent with an additive genetic effect explaining 25% of the variance, whereas approximately 55% was transmissible (cultural+genetic) (Boucth et al., 1988). Subcutaneous fatness (sum of skinfolds) also demonstrates aggregation within families (Table 2.8). Spousal correlations for skinfolds range from 0.02 to 0.46. As for the BMI, this variation may be explained by differential mating patterns and different contributions from the living environment. Parent-offspring and sibling correlations for the sum of skinfolds range from 0.13 to 0.68. Fatness is a complex phenotype and is influenced by many environmental factors, such as energy intake and physical activity. However, the incorporation of activity levels and food intake into genetic models has only a small effect on parent- child similarities (Bouchard et al., 1989). Indicators of fatness still exhibit a significant genetic influence after physical activity and lifestyle variables were considered in an Indian population (Mitchell et al., 1993) and the Quebec Family Study (Savard et al., 1983). 28 Relative Subcutaneous Fat Distribution The distribution of adipose tissue within the body, both subcutaneously (external) and viscerally (internal), is as important a risk factor for many diseases as is the total amount of fat (Ducimetiere et al., 1986; Ducimetiere and Richard, 1989; Després et al., 1990). Evidence is available primarily for familial resemblance in subcutaneous fat distribution in contrast to internal vs external fat distribution, for which little data are available. Relative fat distribution shows familial aggregation (Table 2.8). Spousal correlations are generally similar to those reported for overall fatness, while correlations among first degree relatives vary among studies, ranging from 0.22 to 0.36 for TER and from 0.00 to 0.24 for the WHR. Fatness and relative fat distribution are multifactorial traits which exhibit high individual variability and change dramatically over the lifespan (Malina, 1996). The evidence indicates that there is a significant genetic component to variation in these complex phenotypes. Physique Several studies have investigated the farniliality of physique, as assessed by the Heath-Carter anthropometric somatotype. Somatotype is a three component descriptor of physique, the configuration of the body as a whole. Since the three components, endomorphy, mesomorphy, and ectomorphy. are interconelated, it is important to statistically control for the other two somatotype components in analyses (Song et al., 1993, 1994). Recent studies have used regression analyses to control for the other two components; however, earlier studies by Kovar (1977), Bouchard et al. (1980a) and Perusse et al. (1988) did not control for the effects of the other two somatotype components. There is considerable evidence for familial resemblance in somatotype (Table 2.9). Spousal correlations for specific components are generally low, ranging from - 0.08 to 0.23, whereas sibling and parent-offspring correlations are of a higher magnitude. Sibling correlations range from 0.22 to 0.59 and parent—offspring 29 correlations range from -0.04 to 0.41. There are no apparent trends in correlations by somatotype component; however, mesomorphy appears to demonstrate a higher degree of familial resemblance than the other components (Song et al., 1993; Sdnchez-Andres, 1995). Since spousal correlations are generally lower than sibling and parent-offspring correlations, the transmission of somatotype is probably under some degree of genetic regulation. However, within studies, sibling correlations are generally greater than parent-offspring correlations, suggesting that the shared household environment may also be important in the familial resemblance. Twin studies generally demonstrate higher sibling correlations than family studies (Kovar, 1977; Song et al., 1994). Intraclass correlations among pairs of MZ twins (0.51 to 0.90) are higher than correlations among DZ twins (0.15 to 0.64), indicating that genetic factors are contributing to the variance in somatotype. Strength and Flexibility Familial resemblance in grip strength and trunk flexibility is reviewed subsequently. For a more extensive review of the genetics of performance, the reader is referred to Bouchard et al. (1997). ' Grip Strength The available evidence suggests significant familial aggregation in grip strength (Table 2.10). Spousal correlations range from low to moderate, 0.01 to 0.26, whereas sibling correlations range from 0.10 to 0.55. Parent-offspring correlations are generally of lower magnitude than sibling correlations, ranging from -0.05 to 0.31. Although there is evidence for genetic effects, the role of the shared environment is also important in the familial resemblance in grip strength. Trunk Flexibility Flexibility appears to be highly heritable at least in the broad sense of transmissibility. Perusse et al. (1988) demonstrated a heritability of 0.48 for the sit- and—reach in a stratified sample of the Canadian population, while Devor and Crawford 30 (1984) calculated a transmissibility of 0.66 for the same measure in a sample of Kansas Mennonites. Correlations among family members indicate significant transmissibility of flexibility between parents and offspring, while the shared environment is also important in explaining the variance within and among families (Table 2.10). Using intraclass correlation within pairs of twins, Macs et al. (1993) indicated that MZ twins (0.82) had higher correlations than DZ twins (0.53), suggesting that trunk flexibility is in part genetically determined. The flexibility phenotype is largely a measure of the architecture of the joint and surrounding musculature (Bouchard et al., 1997), which may explain the high degree of concordance among studies. Flexibility is joint specific and heritability estimates of trunk flexibility may not be applicable to other joints. Summary The available evidence suggests significant familial aggregation in body size, adiposity, relative fat distribution and indicators of health-related fitness; however, the magnitude of the family effect varies among studies. Inferences regarding the genetics of the selected phenotypes are difficult to make based on correlational studies, but comparisons of the magnitude of parent-offspring, sibling and spousal correlations suggest the influence of genetic factors. Spousal correlations for all phenotypes indicate that assortative mating may occur in some groups, and that common environmental (household) effects may be important in explaining the familial component of the phenotypic variance. CHAPTER III METHODS Introduction The purpose of this study was to compare Canadians of First Nation and European ancestry in terms of body size, physique, and indicators of health-related fitness, and to determine the familial resemblance in these variables. The protocol, therefore, involved the collection of anthmpometric and health-related fitness data in such a way as to allow statistical familial analyses. The most critical aspect for the analysis is the knowledge of the familial relationships among the subjects, which is why a family study approach was used. Research Location and Study Population The northern Ontario town of Temagami and the First Nation community of Bear Island were selected as the sites for study. The nearest city to these communities is North Bay (pop. 60,000), which is located 100 km south along a national highway. New Liskeard is a farming town of approximately 5,500 people, located 70 km north of Temagami (Figure 3.1). Bear Island is approximately 24 km from Temagami, and can only be reached by water. Each community has a public school (K-8), with 130 children attending the Temagami Public School and 17 attending the Laura McKenzie Learning Centre on Bear Island at the time of the study. Many children are bussed to North Bay or New Liskeard for high school. The exact population of the Temagami area is difficult to determine. According to Statistics Canada, Temagami had 1,030 residents in 1993, based on tax returns with 31 32 an address using the Temagami (POH 2H0) postal code (Statistics Canada, 1995). According to the Corporation of The Township of Temagami, the total population of Temagami was 864 people in 1994 (Township of Temagami, 1994). These two sources provide different estimates of the population of Temagami; however, the area encompassed by the Statistics Canada report is larger than the Township of Temagami proper, as there are many people living outside of the township who get their mail delivered to Temagami. On the other hand, there are also people who are not full-time residents of Temagami who use this address for tax returns (students, seasonal workers, etc.). All things considered, it is likely that the true population of Temagami is between 864 and 1,030 people. The Temagami First Nation has accurate records of the population of Bear Island. Each building on the Island is numbered and the occupants of each building are known. Based on the Band records, the community of Bear Island had 174 residents at the time of this study. Ethnographic Background Present day Temagami, Teme-augaming (the place of deep water),lis inhabited by both Aboriginal and European Canadians. The history of each group in the area is unique. The Aboriginals of the area are the Teme-Augama Anishnabai (the people of the deep water) and are traditionally an Algonkian speaking group. The Teme-Augama Anishnabai have documented 6,000 years of occupation of their homeland, N'Daki Menan (Teme-Augama-Anishnabai, 1990). Their homeland at the time of European contact encompassed approximately 3,800 square miles around Lake Temagami; however, the band was small, numbering fewer than 200 people (Hodgins and Benidickson, 1989). The population of Temagami (Bear Island) in 1913 was 95 people (Speck, 1915). Because of the small population size, the Temagami people had a pattern of "migration” by intermarriage and spread outward through neighboring 33 tribes, thus leading to a distinctive grouping of Ojibwa, Cree and Algonquin settling in the area (Hodgins and Benidickson, 1989). The archeological record reveals a long history of occupation in the Temagami area by prehistoric hunter-gatherers. Paleoenvironmental and geochronological studies indicate that paleo-Indian occupation as early as 10,500 BP is a distinct possibility; however, no sites this old have been found using current survey methods (Gordon, 1990). Evidence of human habitation at 5.0301240 years BP at a site on the Montreal River is the earliest reliable date obtained from a reliable stratified context (Knight, 1977, as cited by Gordon, 1990). Since deglaciation approximately 10,500 years ago, Temagami has undergone dramatic shifts in hydrology, vegetation, and climate (Gordon, 1990). Prehistoric groups inhabiting the area would have had to adapt to these environmental changes. Archeologists are interested in studying cultural adaptations to the changing environment over time using the archeological record; however, biological adaptations to the changing environment should also be considered. The earliest European contacts in the Temagami area were through, the fur trade, and by the late 1800s, the Hudson's Bay Company was established in the area (Mitchell, 1977). Following the fur trade, the late nineteenth century saw lumbermen, missionaries, prospectors, railwaymen, sportsmen and canoeists entering the area ‘ (I-Iodgins and Benidickson, 1989). From this point on, lumbering, mining and tourism became the chief industries of Temagami. A major blow to the recent economy of the area was the closure of Defasco Canada's Sherman Iron Mine and William Milne and Son's lumber mill in 1990. The main industry in Temagami is now tourism and the spin-off labor market (hospitality, commerce, construction). Today, individuals of Aboriginal and European ancestry are living together in the same community: they attend the same churches, shop at the same stores, and work alongside one another in the area's limited industries. Given the differing periods of 34 habitation of the two groups, inferences about biological adaptation to the northern Ontario environment may be made by comparing First Nation and European Canadians in terms of growth and health-related fitness. ' Study Design The design of the study involved collecting anthropometric, performance, and health-related fitness data on a sample of subjects from two ethnically distinct populations inhabiting the same environment. The data were collected in an overarching framework of a family study, in which the purpose was to estimate the heritability of the anthropometric and health-related fitness variables. Data were collected during the spring and summer of 1996 (May-August). All residents of Temagami and Bear Island from 5-75 years of age were eligible to participate in this study. Permission to undertake this study was obtained from the Township Council of Temagarrri, the Temagami First Nation, the Timiskaming Board of Education, and the University Committee for Research Involving Human Subjects (UCRIHS) at Michigan State University. Copies of letters of permission are in Appendix A. All adult subjects signed informed consent forms. In addition, parents were required to sign a consent form for their children (<18 years old) to participate in the study. Children gave their assent by signing the form along with their parents. Subjects were recruited over the telephone and by going door to door (for adults), and using a letter sent home from the principal of the public school (for children 5-15 years). The majority of the children 5-15 years were measured at school, whereas adults were generally measured in their home. Reasons for non-participation included having other time commitments, not knowing about the study (away from home, could not be reached by phone, etc.), and not wanting to be measured. Given that non-participation included not getting measured, it is difficult to determine if there were differences between participants and non-participants in body morphology or health-related fitness which may have biased the results. 35 All subjects were measured for several anthropometric dimensions, grip strength and trunk flexibility. Additionally, children attending the public schools (5-15 years) completed an extended battery of motor fitness tests. Sample A total of 624 subjects, 130 First Nation (FN), 494 European ancestry (EA) participated in the study. The age and sex distribution of the sample is compared to population statistics for Temagami and Bear Island in Table 3.1. Approximately 50% of the total population of the area participated. Some FN people live on the mainland, and some EA people also live on Bear Island. It must be noted that the population statistics include all residents of the area; however, the present sample includes only those individuals 5 through 75 years of age. Thus, the study sample represents more than 50% of the individuals within this age range. Subjects were assigned to either the FN or EA group based on self-ascribed ethnic status, and was not based on biological markers such as blood groupings or skin reflectance. Those who indicated admixture (hybrids) were assigned to the FN group if they had a parent or grandparent who was FN, or if they were classified as a Status Native by the federal government. A higher participation rate was obtained in the public schools over the overall participation rate. Of 130 children attending the Temagami Public School, 108 participated (83%). Additionally, all 17 children attending the Laura McKenzie beaming Centre on Bear Island participated in the study (100%). 36 Anthropometry Several anthropometric dimensions were taken on each subject: stature; sitting height; body mass; skinfolds at the biceps, triceps, subscapular, abdominal, suprailiac, supraspinale and medial calf sites; biacromial, bicristal, biepicondylar, and bicondylar breadths; and flexed and relaxed mid-arm, maximal calf, waist and hip circumferences. All bilateral measurements were taken on the right side of the body. Subischial length was estimated as stature minus sitting height, and arm muscle area was estimated from arm circumference and the triceps skinfold. Several indices were also derived: the body mass index (BMI), Heath-Carter anthropometric somatotype, the sitting height/stature ratio, hip/shoulder breadth ratio, sum of skinfolds, a trunk/extremity skinfold ratio, and the waist/hip circumference ratio. The procedures for the anthropometric dimensions were as follows (Lohman et al., 1988): Body Size Stature (cm) was measured using a field anthropometer (GPM, Switzerland) to the nearest mm with the subject in bare feet, standing on a flat surface with weight evenly distributed on both feet. The head was positioned in the Frankfort Horizontal Plane. Sitting Height (cm) was measured using a field anthropometer to the nearest mm with the subject seated on a table with the legs hanging freely and hands resting on the thighs. The backs of the knees were close to but not touching the table. The subject was sitting as erect as possible with the head in the Frankfort Horizontal Plane. Body Mass (kg) was assessed to the nearest 0.2 kilograms using a spring scale (Medixact Proshape, Sunbeam-Oster, Schaumburg, IL) resting on a hard flat surface. Although the standard technique involves the use of a beam scale, the use of a spring scale is recommended for field work when there is no practical alternative. Body mass was optimally measured with the subject wearing only light shorts and a t-shirt. In those cases where this was impossible, an adjustment for the mass of extra clothing was made based on weighing several individuals in shorts and then in heavy pants or 37 jeans (heavy pants or jeans = 0.90 kg adjustment in adults, 0.45 kg adjustment in children <18 yrs). Skinfolds Skinfolds were measured with Holtain (Holtain LTD, Crymych, U.K.) calipers to the nearest 0.2 mm. Triceps skinfold (mm) was measured over the midline of the triceps muscle, midway between the lateral projection of the acromion process and the inferior margin of the olecranon process. The measurement was made 1 cm proximal to the marked level with the upper extremity hanging loosely with the palm facing forward. Biceps skinfold (mm) was measured as the thickness of a vertical fold raised over the belly of the biceps muscle, 1 cm superior to the line marked for the measurement of me triceps skinfold. The upper extremity was relaxed at the side, palm pointing forward. Subscapular skinfold (mm) was measured just below the inferior angle of the scapula on a diagonal, inclined infero-laterally, approximately 45 degrees to the horizontal plane in the natural cleavage lines of the skin. . ' Abdominal skinfold (mm) was measured as a horizontal skinfold 3 cm lateral to the umbilicus and 1 cm below it. Suprailiac skinfold (mm) was measured in the midaxillary line immediately ~ superior to the iliac crest. The arms hung loosely, or were slightly abducted to facilitate measurement. The skinfold was raised following the natural cleavage of the skin, approximately 45 degrees to horizontal. Supraspinale skinfold (mm) was measured 5 cm superior to the anterior superior spine of the iliac crest along ‘a 45 degree angle to the horizontal in the natural cleavage line of the skin. 38 Medial calf skinfold (mm) was measured with the subject sitting with the knee flexed 90 degrees and the sole of the foot on the floor. This measurement was made along the long axis of the medial aspect of the calf at the point of maximal calf girth. Breadths and Circumferences The following bony breadth measurements were made to the nearest mm by applying firm pressure with the upper end of the anthropometer. Biacrornial breadth (cm) was measured with the subject standing. The measurement was made from behind as the distance between the most lateral aspects of the acromial processes. Bicristal breadth (cm) was measured with the subject standing with the feet about 5 cm apart and the arms folded across the chest. The measurement was made from behind at the widest biiliac breadth. Bicondylar breadth (cm) was measured with the subject seated with the knee flexed 90 degrees. The distance between the lateral and medial condyles of the femurs was measured. The following measurement was made to the nearest mm by applying firm pressure with a small sliding caliper (GPM, Switzerland). Biepicondylar breadth (cm) was measured with the subject's arm raised to the horizontal, and the elbow flexed 90 degrees. The measurement was made between the lateral and medial epicondyles of the humerus. The following measurements were made to the nearest mm with a Grafco flexible fiberglass tape. Relaxed mid-arm circumference (cm) was measured with the subject standing with arms hanging at the sides, palms facing the thighs. This measurement was made at the midpoint between the acromion process of the scapula and the inferior margin of the olecranon process of the ulna. 39 Flexed mid-arm circumference (cm) was measured with the subject standing. The subject was instructed to flex the biceps or to "make a muscle" with the right arm. This measurement was made at the point of maximal circumference of the flexed arm. Maximal calf circumference (cm) was measured with the subject standing and weight evenly distributed on both feet. The tape was placed around the calf and moved up or down to locate the maximal girth, at which point the measurement was recorded. Waist circumference (cm) was measured with the subject standing and weight distributed evenly on both feet, arms at the sides and abdomen relaxed. This measurement was rmde over one layer of light clothing (t-shirt, dress) at the natural waist, which is the narrowest part of the torso. In obese subjects it was necessary to move the tape up and down and record the smallest horizontal circumference between the ribs and the iliac crest. Hip (buttocks) circumference (cm) was measured with the subject standing erect with arms at the sides and feet together. The measurement was taken at the level of maximum extension of the buttocks in a horizontal plane. This measurement was taken through one layer of clothing. 40 Anthropometric Indices The derived indices were as follows: Subischial length (SIL), or estimated leg length was estimated as stature minus sitting height. Arm Muscle Area (AMA) was estimated using the following formula (Frisancho, 1990): [C-(nYSFM’ AMA (cm’) = 4n: where C is arm circumference (cm) and TSF is the triceps skinfold (cm). Body mass index (BMI) was calculated as body mass/stature2 (kg/m2). Sitting height/stature ratio (SSR) was calculated as sitting height/stature x 100. Hip/shoulder ratio (HSR) was calculated as bicristal breadth/biacromial breadth x 100. Sum of trunk skinfolds (TRUNK) was calculated as the sum of 3 trunk skinfolds (subscapular, abdominal, and suprailiac). Sum of extremity skinfolds (EXTREMITY) was calculated as the sum of 3 extremity skinfolds (biceps, triceps, and medial calf). Sum of skinfolds (SUM) was calculated as the sum of 6 skinfolds (subscapular, abdominal, suprailiac, biceps, triceps, and medial calf). Trunk/extremity ratio (TER) was calculated as the ratio of the sum of the subscapular, abdominal, and suprailiac skinfolds to the sum of the biceps, triceps, and medial calf skinfolds. Waist/hip ratio (WHR) was calculated as the ratio of waist circumference to hip circumference. 4i Somatotype Somatotype was derived using the equations of Carter and Heath (1990). Endomorphy Endomorphy = -0.7182 + 0.1451 (A) - 0.00068 (A2) + 0.0000014 (A3) where A: [(triceps (mm) + subscapular (mm) + supraspinale (mm) skinfolds) x (1701.8lstature (cm)] Mesomorphy Mesomorphy = [0.858 x biepicondylar breadth (cm)] + [0.601 x bicondylar breadth(cm)] + [0.188 x CAG] + [0.161 x CCG] - [stature (cm) x 0.131] + 4.50 where CAG = corrected arm girth = flexed arm circumference (cm) minus triceps skinfold (cm), and CCG = corrected calf girth = maximal calf circumference (cm) - medial calf skinfold (cm) Ectomorphy If SMRZ 40.75, Ectomorphy == SMR x 0.0732 '- 28.58 If SMR < 40.75 but > 38.25, Ectomorphy = SMR x 0.0463 - 17.63 If SMR 5 38.25, Ectomorphy = 0.1 where SMR = stature mass ratio = eta: 3:3) If any somatotype component is zero or negative, a value of 0.1 is assigned (Carter and Heath, 1990). 42 Strength, Flexibility, and Motor Fitness Adolescents and Adults (16-75 yrs) Each adolescent and adult performed a strength test and flexibility test. Left and right grip strength of the hand was measured with a. Stoelting adjustable dynamometer (Stoelting Co., Chicago, IL) to the nearest 0.5 kg following the procedures of the Canadian Standardized Test of Fitness (Fitness Canada, 1986). The subject held the dynamometer in line with the forearm at the level of the thigh. The dynamometer was then squeezed vigorously so as to exert maximum force. Three trials were allowed with each hand, the best one being retained in the analysis. Trunk flexibility was assessed using a sit-and-reach exercise following the procedures of the AAHPERD Physical Best manual (1988). The subject was seated with the legs fully extended and feet pressed up against the test apparatus. The subject extended forward with the hands placed on top of one another to perform the test. Each participant was allowed three trials in which they had to hold the position for at least. one second. Flexibility was measured to the nearest 0.5 cm. A measurement of 23 cm corresponds to touching the toes. Children (5-15 years) Children were tested on a larger number of items than adults. The following additional fitness tests were administered during regular class time at the Temagami Public School and the Laura McKenzie Learning Centre on Bear Island: right and left grip strength, trunk flexibility, flexed arm hang, sit-ups, standing long jump, and 35-meter dash. Only children with signed parental consent forms participated in this study. All items in the battery of fitness tests were repeated 3 times and the best performance was retained for analysis. Right and left grip strength was assessed as in adults. Trunk flexibility was assessed as in adults. 43 A Flexed arm hang was conducted on a bar parallel to the ground, following the protocol of the Leuven Growth Study of Flemish Girls (Claessens et al., 1990). Hands were in the pronated position, and the time was stopped when the eyes dropped below the level of the bar. Time was recorded to the nearest second. Sit-ups were assessed following the protocol of the Canadian Standardized Test of Fitness (Fitness Canada, 1986). The subject was in a supine position with legs flexed 90 degrees at the knee. The researcher held the subject's ankles to assure that the heels remained in contact with the floor. With hands placed beside the head with fingers over the ears, the subject touched the knees with the elbows and returned to the starting position as many times as possible in 60 seconds. The Standing long jump was assessed by having the subject jump as far as possible from the standing position into a jumping sand pit. Distance from take-off to the back of the heels on landing was measured to the nearest 0.01 m. A 35-meter dash was conducted from a stationary start position and was measured to the nearest 0.1 second. 44 Measurement Variability and Reliability In order to estimate measurement variability and demonstrate reliability, several considerations were made in devising the protocol for this study. Measurement Variability of Anthropometry , Replicate anthropometric dimensions were taken on a subsample of approximately 10% of the study sample (64 subjects). The replicate measurements were made at least 1 day apart and no more than 2 months apart (mean 10 days). The intro-observer technical error of measurement was calculated using the following formula (Malina et al., 1973): TEM= M where d2 is the sum of the squared differences of replicate measurements and Zn represents twice the number of pairs. Additionally, reliability coefficients (intro-class correlation coefficients) were calculated for the anthropometric measurements. One- way analysis of variance was used to obtain the mean square among subjects (MS A) and the mean square within subjects (MSW). The formula for the intraclass correlation coefficient is as follows (Baumgartner and Jackson, 1991): rm: (MSA - MSW) I MS A The closer the intraclass correlation coefficient is to one, the smaller the error introduced by the observer. Table 3.2 presents the age and sex distribution of the subsample used in this analysis. The average age of the sample was 25.4 years, ranging from 4.4 to 75.3 years. Descriptive statistics are not presented due to the wide range of ages represented in the subsample. The mean differences between replicate measures are presented with the intra- Observer TEMs and intraclass correlation coefficients in Table 3.3. Table 3.4 compares the intra-observer TEMs with those from selected studies (Malina, 1995). The intra- Observer error in this study is similar to that of the Hispanic Health and Nutrition Examination Survey (Chumlea et al., 1990) and the National Health and Nutrition 45 Examination Survey (Johnston et al., 1972; Malina et al., 1973). The TEMs reported by Siegel (1995) and Klika (1995) are quite low; however these studies involved athletic subjects within a small age-range. The sample of the present study is comparable in age range to the national surveys described above; thus the intra-observer TEM is of similar magnitude. The technical errors of measurement for the somatotype components were 5 0.2 somatotype components, which compare to those reported for the Quebec Family Study (5 0.3 somatotype units; Bouchard, 1985). Reliability of Strength, Flexibility and Motor Fitness Within day reliabilities of the fitness tests were estimated using intraclass correlation coefficients (rm) between the best versus the second best trial of the three trials which were achieved for each test (Baumgartner and Jackson, 1991). Intraclass correlations for the strength, flexibility and motor fitness variables are presented in Table 3.5 along with the sample sizes used for the calculation of each correlation. Intraclass reliability coefficients are compared to selected studies in Table 3.6. The fitness tests in the present study are very reliable, demonstrating intraclass correlations greater than or equal to 0.99 for all variables. Given that the intraclass correlations were calculated over a large age range, it is not surprising that they appear higher than those of the other studies, which use single year age groups. 46 Data Management Each subject was identified by an ID number and data were entered into a database management system (Dbase III). A file was created which contained the subject id number, parental id numbers, sex, ethnicity, birth and observation dates, and all anthropometric and performance data collected on each subject. All data were double entered into a second Dbase 111 file, which was merged with the original file using SPSS procedures (SPSS, 1990). An SPSS program was written to compare the two Dbase III files and flag discrepant values. Errors were corrected in the Dbase III files until no discrepant values were found. The corrected Dbase 1]] file containing all anthropometric and performance data was converted into an SPSS system file which was retained for further analysis. SPSS programs were written to compute fractional ages and anthropometric indices based on existing data. Missing data were coded as system missing values with the exception of single skinfolds. For individuals who were missing a single skinfold, this value was predicted using multiple regression from the subject’s age, sex and the remaining six skinfolds. This procedure resulted in increasing the sample size for anthropometric indices which have skinfolds as a component. A total of 25 individual skinfolds were predicted in adults, none in children. Table 3.7 demonstrates the prediction equations and the standard errors of the estimates for the predicted skinfolds (abdominal, subscapular, and medial calf). For the purpose of identifying outliers, examining distributions for normality, and adjusting values for age, the sample was split into four groups based on age and sex. The groups were as follows: males 219.50, males $19.49 years, females 219.50 years, and females $19.49 years. Frequency distributions for all variables were examined for normality, and those variables significantly skewed were loglo transformed where appropriate. The variables demonstrating skewed distributions and 47 their associated skewness statistics are presented in Table 3.8. For the comparative analyses between EA and FN samples, the log,0 transformed values were used in place of the raw data. Outliers were considered to be values falling beyond 4 standard deviations of each age group mean (see above) and were eliminated from further analysis. The elimination of outliers resulted in the loss of 29 values from 11 subjects. Regression analyses were used to adjust raw data for the effects of age within each of the agchex groups defined above for the purpose of the familial aggregation analyses. Scores were adjusted by applying the following multiple regression: Y = age + age2 + age3 The residuals of the multiple regression were retained for further analysis, and were considered to be adjusted for the effects of age and sex . Since somatotype is a three component descriptor, each somatotype component was further adjusted for the effects of the other two components by regression procedures (Song et al., 1993).' Tables 3.9 and 3.10 present the variability in the original variables which was accounted for by the adjustment procedures. For the purpose of the familial aggregation analyses, the data were reorganized into parent and offspring samples. A total of 266 nuclear families were differentiated following the reorganization. Variables were then z-standardized within generations which served to normalize the distributions of several skewed variables. The offspring generation was further reorganized into sibships based on parental id numbers. The distribution of sibship size is presented in Table 3.11. 48 Statistical Analyses The analyses for the specific hypotheses are as follows: Hmthcsiu There are significant differences between Canadians of First Nation (FN) and European ancestry (EA) in body size, physique, and indicators of health-related fitness. Hypothesis 1a) FN Canadians are heavier and demonstrate greater subcutaneous fatness than EA Canadians throughout childhood into adulthood. Analyses: Within age/sex groups, indicators of heaviness (body mass, BMI) and subcutaneous fatness (SUM, TRUNK, EXTREMITY) were compared between FN and EA subjects using AN COVA with age as a covariate. Individual anthropometric z- scores were calculated for body mass, the BMI, triceps and subscapular skinfolds, and AMA using the following equation (Gibson, 1990; 251): individual’s value - median value of reference population z score: standard deviation value of reference population The reference data used for body mass were from Health and Welfare Canada (1980), while data fOr the BMI, and triceps and subscapular skinfolds were from the NHANES II for the U.S. population (Najjar and Rowland, 1987). AMA was z-standardized using the reference data of Frisancho (1990) for U.S. Whites based on NHANES I and NHANES 11. Additionally, z—scores for body mass in FN subjects were also calculated using First Nation reference data from Health and Welfare Canada (1980). Since the conversion of data into z-scores theoretically eliminates the function of age, independent samples t-tests were used to make comparisons among FN and EA subjects, and between males and females in terms of z-transformed variables. Prevalence rates for obesity were calculated for FN and EA samples by age and sex group. Two indicators of obesity were used: theBMI and the triceps skinfold (TSF). For subjects 5-19 years of age, the criteria for obesity based on the BMI and 49 TSF was greater than or equal to the respective age- and sex-specific 85th percentiles of NHANES II data (N ajjar and Rowland, 1987). For subjects 20-75 years of age, the criteria for BMI obesity was 2 85th percentile NHANES II data for 20-29 year old people (BMI 2 27.8 in males and BMI 2 27.3 in females) and the criteria for TSF obesity was 2 85th percentile NHANES II data for 18-24 year old people (TSF 2 17.5 mm in males and TSF 2 29.5 mm in females). Subjects were classified as obese by both the BMI and TSF independently. Additionally, subjects were classified as obese by the BMI only (BMI Obese), TSF only (TSF Obese), or both the BMI and TSF together (BMI+TSF Obese). This classification is based on Malina et al. (1989), which generally corresponds to the scheme of Van Italic and Abraham (1985): “overweight not obese” - high BMI, low skinfolds, “obese not overweight” - high skinfolds, low BMI, and “overweight and obese” - high BMI, high skinfolds. Characteristics of subjects in each classification of obese were compared in terms of selected bony dimensions, relative fat distribution, and somatotype. Hypothesis lb) There are significant differences in relative fat distribution'and physique between FN and EA Canadians. Analyses: Within age/sex groups, the TER, an indicator of relative subcutaneous fat distribution, and the WHR, an indicator of central/peripheral fat distribution, were compared between FN and EA subjects within age/sex groups using ANCOVA, with age as a covariate. Heath-Carter anthropometric somatotypes were compared using the protocol of Cressie et a1. (1986), which involves three steps. Since somatotype is a three component index, each component should not be considered as a separate variable. The first step in the analysis was to perform an overall MANCOVA between the groups with age as a covariate. For those comparisons demonstrating a significant 50 MANCOVA, univariate F-tests were performed to determine which components were contributing to the significant difference. Finally, for those groups demonstrating a significant MAN COVA, a forward discriminant function analysis was performed to determine which somatotype components best distinguish between the two groups. 1c) There are no differences in stature and other skeletal dimensions between FN and EA Canadians. Analyses: Stature, sitting height, the SSR, the HSR, and skeletal dimensions were compared between FN and EA subjects within age/sex groups using ANCOVA, with age as the covariate. Anthropometric z-scores were calculated for stature and the SSR using reference data from Health and Welfare Canada (1980). Additionally, z-scores were calculated for stature and the SSR among FN subjects using First Nation reference data from Health and Welfare Canada (1980). Differences in anthropometric z-scores between FN and EA subjects, and between males and females were assessed using independent samples t-tests. HmhcaiLZ Secular trends in body size are evident in FN and EA Canadians. Hypothesis 211) There are significant secular increases in stature, mass and the BMI in FN and EA Canadians. Analyses: This hypothesis was tested two ways. First, comparisons of stature among several samples collected in Canada over the last 35 years were made. Second, the approach of Himes and Mueller (1977a) was used for an internal analysis of statural changes within the FN and EA samples. The technique of Himes and Mueller involves two steps. The first step was to estimate statural losses due to aging, which was accomplished by regressing stature on age, controlling for subischial length (SIL). The 51 partial regression coefficient was retained as the shrinkage factor. Stature of all individuals aged 30 years and older was adjusted by the shrinkage factor. Age 30 was chosen as the age representing maximal stature as some growth may occur into the mid- twenties, especially in males (Trotter and Gleser, 1951; Hertzoget al., 1969). The second step was to regress the adjusted stature estimates on age, and then to use the regression coefficient as an estimate of the secular change. An internal secular trend analysis of body mass and the BMI was not possible due to the natural tendency for these variables to increase with age. This hypothesis was tested by comparison of body mass and the BMI among several samples in Canada over the last 35 years. HaunthcsiLl There is significant familial resemblance in body size, physique and indicators of health-related fitness in FN and EA Canadians. Hypothesis 3a) There is significant familial resemblance in body size, physique and indicators of health-related fitness in FN and EA Canadians. Analyses: The assessment of familial resemblance in the traits of interest was approached using correlation and regression analyses. The following four approaches were used: 1) Intraclass sibling correlations were computed using the ANOVA procedures of Donner and Koval (1980, see also Donner and Eliasziw, 1991). This analysis was limited to 106 sibships with at least 2 siblings. The following summarizes the ANOVA: Sum of Mean - Scarce df SQW F Between Sibships n -1 888 M88 MSB/MSW Within ..Sihshins J- n SSW MSW where n is the number of sibships, 52 k = 2k. , the total numur of observations. 18] The ANOVA estimator of the sibling correlations is defined as: g MSB-MSW MSB+(lr.-1)MSW ’ r1... where 1 1" k.=—k—— k2. n_1( k; .1 2) Pearson interclass spousal correlations were computed between each set of parents. 3) Pearson interclass parent-offspring correlations were computed between each parent and offspring in the following combinations: father-son, father-daughter, father- offspring, mother-son, mother-daughter, and mother-offspring using the pairwise estimator method (Donner, 1979). 4) The regression of offspring on mid-parent (mother + father / 2) values was done to estimate heritability (h’). Using this approach the regression coefficient is the estimator of h’. It must be noted that the regression coefficient obtained is not directly comparable to the correlation coefficients obtained using the previous approaches. Hypothesis 3b) Estimated heritabilities for strength and flexibility are greater after body morphology is factored into the analyses as a covariate. Analyses: Estimates of familial aggregation for grip strength and heritability were computed as described above. Age-adjusted grip strength and flexibility scores were then adjusted for body mass, stature, and the BMI independently using regression procedures. The amount of variability accounted for by age, body mass, stature, and the BMI on grip strength and flexibility using regression techniques is presented in Table 3.12. The four estimates of familial aggregation were then recalculated using the values adjusted for body size. CHAPTER IV RESULTS Introduction The results are presented in two sections. The first section presents descriptive statistics for the sample and comparisons with reference data, while the subsequent section presents results of analyses related to specific hypotheses. For descriptive purposes, the sample was divided into the following sex- specific age groups: 5-9, 10-14, 15-19, 20-29, 30-39, 40—49, 50-59, 60—69, and 70-75 yrs. The age groups were chosen to correspond With those used in national Canadian surveys (Health and Welfare Canada, 1980; Fitness Canada, 1983, 1985, 1986). However, for the statistical analyses, the sample could not be broken down into the same age categories due to insufficient numbers in the Fust Nation group. The age groups used in the analyses were 5-19 years and 20-75 years and age was incorporated into each analysis as a covariate. Descriptive Statistics Tables 4.1 through 4.7 present descriptive anthropometric data for all variables measured and derived. Tables 4.8 and 4.9 present sample sizes, means and standard deviations for the health-related fitness measures. These data are presented for descriptive purposes and are compared to the most recently available reference data for Canada, andattimestoU.S. datawhentherearenocorrespondingreferencedatafor Canadians. 53 Stature Stature was compared to Canadian reference data from the Canada Fitness Survey (Fitness Canada. 1985, 1986). Stature of EA and FN males 5-19 years is presented in Figures 4.1 and 4.2. Stature is between the 10th and 90th percentiles until about ages 10-12 years, when it appears to increase relative to the reference values in EAmales,andapproximatesthemedianinFNmales. 5-yearagegroupmeans approximate the reference mdians (Figure 4.3 and 4.4). Stature of EA and FN males reaches a peak between ages 20-25 and then slowly declines throughout adulthood (Figures 4.5 and 4.6). Adult 10-year age group means fall between the 50th and 90th percentiles. Mean stature of EA and FN females track at the 50th percentile throughout adulthood, and the distribution of statures overlaps the reference data (Figures 4.7 and 4.8). Body Mass Body mass was compared to Canadian reference data from the Canada Fitness Survey (Fitness Canada, 1985, 1986). Body mass in BA males 5-19 years follows the same pattern as stature (Figure 4.9). It approximates the 50th percentile tmtil about age 10-12 and then begins to climb relative to the reference data. F'N males are heavy relative to the reference data throughout childhood (Figure 4.10). The body mass ofEA and FN females 5-19 years (Figures 4.11 and 4.12) generally approximates the 50th percentile in EA females until about age 10-12 when variability increases. Body mass in FN females generally falls between the 10th and 90th percentiles. Body mass remains high throughout adulthood in EA and FN males, with 10- year age group means generally between the 50th and 90th percentiles of the reference data (Figures 4.13 and 4.14). Similarly, 10-year age group means for body mass in BA females track between the 50th and 90th percentiles (Figure 4.15). The body 55 masses of FN females are high relative to the reference data, with lO—year age group means approximating the 90th percentiles throughout adulthood (Figure 4.16). Sitting Height Sitting height was compared to reference data from the U.S. for children and youth (Hamill et al., 1973; Malina et al., 1974) and from the Nutrition Canada Survey for adults (Health and Welfare Canada, 1980). Sitting height in BA and FN males 5-19 years follows the same trend as stature (Figures 4.17 and 4.18). The 5—year age group means generally approximate the reference medians, but sitting height increases relative to the reference in BA males after about age 12. Sitting height of EA females 5-19 years follows reference medians, whereas 5-year age group means in FN females 5- 19 years are between the 50th and 90th percentiles (Figures 4.19 and 4.20). The distribution of sitting heights in adult EA and FN males overlaps the distribution of the reference data, and values decrease throughout adulthood (Figures 4.21 and 4.22). Similarly, sitting height in adult EA and FN females approximates the reference medians (Figures 4.23 and 4.24). Subischial Length Estimated leg length (SIL) was compared to reference data from the U.S. for children and youth (Hamill et al., 1973; Malina et al., 1974). In all age and sex groups, SIL approximates the reference medians throughout childhood and adolescence, but in EA males, SIL climbs relative to the reference in late adolescence (Figures 4.25 through 4.28). I Sitting Height/Stature Ratio The sitting height/stamre ratio (SSR) was compared to reference data from the U.S. for children and youth (Hamill et al., 1973; Malina et al., 1974) and from the Nutrition Canada Survey for adults (Health and Welfare Canada, 1980). A significant number of EA and FN males 5-19 years fall below the 10th percentile for SSR, indicating that they are relatively long-legged compared to the reference (Figures 4.29 56 and 4.30). The 5-year age group means fall between the 10th and 50th percentiles. Figures 4.31 and 4.32 present corresponding data for the SSR in EA and FN females 5-19 years. In EA females, 5-year age group means are between the 10th and 50th percentiles, and they are slightly higher in FN females, although there is considerable variability in the data. Throughout adulthood, lO-year age group means for SSR are below the 50th percentile in BA males (Figure 4.33). There is considerable variability in the SSR of FN males 20-75 years; however, lO-year age group means approximate the median (Figure 4.34). The 10-year age group means for SSR in EA and FN females tend to fall between the 10th and 50th percentiles throughout adulthood, although the distributions overlap the reference data (Figures 4.35 and 4.36). Arm Muscle Area Estimated arm muscle area (AMA) was compared to U.S. reference data for Whites from NHANES I and II (Frisancho, 1990). AMA in BA and FN males 5-19 years approximates the reference medians (Figures 4.37 and 4.38). Similarly, 5-year AMA means in EA and FN females 5-19 years also approximate the medians (Figures 4.39 and 4.40). The AMA of adults is presented in Figures 4.41 through 4.44. In all sex/ethnic groups, the distribution of AMAs overlaps the distribution of the reference data. Similarly, 10-year age group means in all sex/ethnic groups approximate the reference medians. BMI The BMI was compared to U.S. reference data from NHANES II (Najjar and Rowland, 1987). In EA males 5-19 years, the distribution of the BMI is bimodal, with many subjects between the 10th and 50th percentiles, and many individuals at the upper extreme of the distribution, >90th percentile (Figure 4.45). Similarly, the BMI of FN males 5-19 years is generally between the 10th and 90th percentiles, with some subjects 57 exceeding the 90th percentile (Figure 4.46). With few exceptions, the BMI of EA females 5-19 years falls between the 10th and 90th percentiles of the reference data, and 5-year age group means approximate the median (Figure 4.47). FN females 5-19 years also demonstrate a distribution of the BMI that extends from the. 10th percentile to >90th percentile (Figure 4.48). There are a significant number of EA males 20-75 years with a BMI >90th percentile throughout the age range (Figure 4.49). FN 20-75 years are also heavy, with lO-year age group means falling between the 50th and 90th percentiles (Figure 4.50). The BMI of adult EA females follows the same pattern as adult EA males; 10- yearagegroupmeansarebetweenthe 50thand90thpercentiles,andtherearealarge number of people with a BMI >90th percentile (Figure 4.51). Similarly, FN females 20—75 years are also heavy, with lO-year age group means falling between the 50th and 90th percentiles (Figure 4.52). Triceps Skinfold The triceps skinfold was compared to U.S. reference data from NHANES II (Najjar and Rowland, 1987). The distribution of the triceps skinfold in EA males 5-19 years overlaps that of the reference data, with values ranging from <10th to >90th percentile. Similarly, 5-year age group means fall between the 50th and 90th percentiles (Figure 4.53). FN males 5-19 years also have triceps skinfolds which generally fall between the 10th and 90th percentiles for ages 5-19 years (Figure 4.54). Note that the 10th and 50th percentiles of the reference data are relatively stable; however, the 90th percentiles vary with age in males 5-19 years. As in males, the distribution of the triceps skinfold in EA females approximates the distribution of the referencedata,andthoseofFNfemalesaregenerallywithinthe10thand90th percentiles (Figures 4.55 and 4.56). Adult lO-year age-group means for the triceps skinfold approximate the reference medians throughout adulthood in EA males and females (Figures 4.57 and 58 4.59). Although the distribution of the triceps skinfold of FN males and females overlaps the reference distribution, 10-year age group means tend to fall above the medians (Figures 4.58 and 4.60). Subscapular Skinfold . The subscapular skinfold was compared to U.S. reference data from NHANES II (N ajjar and Rowland, 1987). The distribution of the subscapular skinfold in both EA and FN males is bimodal in the 5-19 year age group, with most of the sample falling between the 10th and 90th percentiles, and another group falling above the 90th percentile (Figures 4.61 and 4.62). The distribution of subscapular skinfolds in BA females is similar to that of EA males, such that it is skewed towards the upper extremes (Figure 4.63). The subscapular skinfolds of FN females generally fall between the 10th and 90th percentiles of the reference data (Figure 4.64). In adulthood, 10-year age group means for the subscapular skinfold are between the 50th and 90th percentiles for EA and FN males, with a large number of subjects above the 90th percentile (Figures 4.65 and 4.66). Although the distributions of the subscapular skinfold overlap the reference distributions, adult EA and FN female 10-year age group means are between the 50th and 90th percentiles of the reference data (Figures 4.67 and 4.68). Grip Strength Combined grip strength (right-Heft grip) was compared to reference data from the Canada Fitness Survey (Fitness Canada, 1985, 1986). There is a linear relationship between grip strength and age in males and females 5-19 years, and no apparent differences between EA and FN children. Values approximate the medians of the reference data in all sex/ethnic groups (Figures 4.69 through 4.72). Amongadults,10-yearagegroupmeansinEAmalesareabovethe50th percentile, whereas corresponding means of FN males are below the 50th percentile, with the exception of the 20-29 year age group (Figures 4.73 and 4.74). In contrast, 59 means for EA and FN adult females are above the 50th percentile and the distributions overlap considerably (Figures 4.75 and 4.76). Trunk Flexibility Trunk flexibility was compared to reference data from the Canada Fitness survey (Fitness Canada, 1985, 1986). There is no apparent relationship of flexibility with age, and values generally fall between the 10th and 90th percentiles of the reference data for children 5-19 years (Figures 4.77 through 4.80) . The distributions of trunk flexibility in adults overlap the distributions of the reference data, and both EA and FN 10-year age group means approximate the 50th percentile throughout adulthood (Figures 4.81 through 4.84). There are no apparent differences in the distribution of flexibility scores among EA and FN groups. Sit-ups The number of sit-ups performed in 60 seconds was compared to reference data from the Canada Fitness Survey (Fitness Canada, 1985, 1986). The number of sit-ups increases with age in both males and females, and the distributions of EA and FN children overlap considerably (Figures 4.85 through 4.88). Compared to the reference data, EA and FN children perform poorly, with 5-year age group means falling between the 10th and 50th percentiles in all sex/ethnic groups. Flexed Arm Hang ThetimedflexedarmhangwascomparedtodatafromtheMichiganState University Motor Performance Study (Haubenstricker et al., 1991). Performance increases with age in BA males (Figure 4.89), but does not show a linear trend with age in FN males (Figure 4.90). In females, the age related increase in the reference data is not as marked as in the males (Figures 4.91 through 4.92). The distribution of values in EA and FN females overlaps the distributions of the reference data, and the distributions of EA and FN children overlap. considerably. Although low, the values of EA and FN children fall within the range for U.S. (Michigan) children. 60 235-meter Dash Running speed (m/s) in the 35 meter dash was compared to running speed (m/s) for a 27.5 ureter dash in the Michigan State University Motor Performance Study (Haubenstricker et al., 1991). Running speed increases linearly with age in boys and girls (Figures 4.93 through 4.96). There are no apparent differences between EA and FN children in speed; however, mean running speed is at or below the 10th percentile of the reference data in both EA and FN boys and girls. Standing Long Jump The standing long jump was also compared to data from the Michigan State University Motor Performance Study (Haubenstricker et al., 1991). Performance increases linearly with age in BA and FN boys and girls, but the s10pes vary (Figures 4.97 through 4.100). There are no apparent differences between EA and FN children. Relative to U.S. (Michigan) children, performance of the EA and FN children generally falls between the 10th and 50th percentiles. 6i Anthropometric Z-Score Analysis Anthropometric z-scores indicate that both EA and FN males are tall and heavy relative to Canadian reference data (Health and Welfare Canada, 1980). Mean z-scores for stature in males range from 0.42 to 0.98, and those for mass in males range from 0.68 to 1.44 (Table 4.10). Relative to U.S. NHANES II data (N ajjar and Rowland, 1987), male BMIs are also greater, demonstrating mean z-scores ranging from 0.40 to 0.82. Mean z-scores for stature in females are consistently positive and similar in magnitude as males, ranging from 0.41 to 0.75. Female z-scores for mass are more variable among age and ethnic groups, ranging from -0.43 in EA females 20-75 years to 1.11 in FN females 5-19 years. Female BMI z-scores are also similar in direction and magnitude as males. BMI z-scores range from 0.26 to 0.99 in females. Table 4.10 demonstrates that the SSR is consistently lower than Canadian reference data in all age and ethnic groups, values ranging from —0. 10 to -0.73, indicating that the subjects have relatively long lower extremities compared to the reference. Subcutaneous fatness shows considerable variability in distribution of z-scores of the triceps and subscapular skinfolds. Triceps skinfold z-scores are similar to those for the BMI in 5-19 year males (EA 0.45, FN 0.80). In males 20-75 years, triceps z— scores are lower than in the younger age group (EA 0.13, FN 0.31). Subscapular skinfold z-scores are also positive in males, ranging from 0.44 in BA 5-19 year males to 1.47 in FN 5-19 year old males. Among females, triceps skinfold z-scores are low and positive, whereas subscapular z-scores are positive and greater in magnitude within each age/sex group. There are significant ethnic differences in anthropometric z-scores within age groups. In 5-19 year old males, FN boys are significantly taller, heavier and demonstrate greater subscapular skinfolds relative to the reference values (p50.05). In 20-75 year old females, the FN sample demonstrates a significantly higher mass, BMI, 62 triceps and subscapular skinfolds, and AMA than the EA sample relative to the reference (p50.05). There are also significant sex differences in anthropometric z-scores within age groups. In the 5-19 year old EA sample, males are significantly taller than females relative to the reference (pS0.05). In 5-19 year old FN subjects, males have significantly greater triceps and subscapular skinfolds than females relative to the reference (p50.05). In the 20-75 year old EA and FN samples, males are significantly heavier than females relative to the reference (pS0.05). Anthropometric z-scores of FN adults standardized against Canadian FN reference data (Health and Welfare Canada, 1980) are presented in Table 4.11. Mean z-stature in FN males is 0.70 standard deviations above the Canadian national reference. Similarly, mass in FN males is 1.38 standard deviations above the reference. In FN females, z-scores for stature and mass are also positive (z- staturmO.98, z-mass=0.69). The mean z-score for mass in males is significantly greater than in females (p50.05). There are no sex differences in FN SSR z-scores, which are zero and negative in both males and females, respectively (males 0.01, females -0.37). Caution must be used in interpreting z-scores derived from reference data from various sources such as above. Secular trends may have occurred between studies which make interpretation of the absolute values of z-scores difficult. Given that comparisons within a given trait were made using the same reference, the temporal trends in the reference data do not play a role in interpreting the results. 63 Secular Trend Analysis for Stature The regression of stature on age in adults 20-75 years indicates a significant decrease in stature with age in EA males and females (Table 4.12). Estimated rates of decrease are 0.21 cm/year in BA males (p<0.001) and 0.10 cm/year in EA females (p=0.002). The regression coefficients for FN adults, in contrast, are not significant, suggesting no secular change in stature. Based on partial regression coefficients for stature on age, controlling for SIL, shrinkage estimates are significant in BA males and females. Statural loss due to aging is estimated at 0.12 cm/year in males (p<0.001) and 0.06 cm/year in females (p<0-001). After adjustment for shrinkage due to aging, a significant secular trend for stature is apparent in BA males. Estimated secular increases in stature adjusted for age- related shrinkage are 1.0 cmldecade in EA males (p<0.005) and 0.4 cmldecade in EA females (p=0.19, ns). 64 Stature, Skeletal Dimensions and Circumferences Ethnic Differences Table 4.13 presents results of the ANCOVAs for differences in stature, skeletal dimensions and AMA between EA and FN. There are few differences in stature and skeletal dimensions. Stature, the SSR and AMA are not different in any age and sex group. Among males 5-19 years, the FN sample demonstrates significantly greater biacromial breadth and HSR (p50.03). In males 20-75 years, the only significant difference is biepicondylar breadth, which is greater in the FN sample (p=0.05). Among females, only the 20-75 year age group demonstrates significant differences in skeletal dimensions. Bicondylar, biepicondylar, biacromial, and bicristal breadths, as well as the HSR are greater in FN females (p50.02). Sex Differences Males are significantly taller in all age and ethnic groups, except for the 5-19 year FN subjects, where the difference is not significant (Table 4.14). Females demonstrate higher SSRs than males in all groups; however, only the 20-75 year EA sample shows a significant difference in the SSR (p<0.001). In all age and ethnic groups, males have significantly greater biepicondylar, bicondylar and biacromial breadths than females (p50.05). Bicristal breadth is only greater in males in the 20-75 year EA sample (p=0.003). In all age and ethnic groups, females have a higher HSR than males, and males have greater estimated AMA (p50.05). Familial Resemblance Stature, skeletal dimensions, circumferences, and AMA were examined for familial resemblance. Intraclass sibling correlations for stature are 0.53 in the total sample, 0.66 in the EA sample, and 0.79 in the FN sample (p<0.001), indicating significant aggregation of stature within sibships (Table 4.15). Similarly, sibling correlations for sitting height, SSR, SIL, biacromial breadth, and AMA also demonstrate significant aggregation within sibships in the EA, FN, and total sample 65 (p<0.05). Sibling correlations are also significant for all of the other skeletal breadths and circumferences in the EA and total samples, but not the FN sample (Table 4.15). The non-significant results in the FN group are probably related to the small samples. There is no evidence for assortative mating for stature, skeletal dimensions, circumferences or AMA in this Northern Ontario population. Spousal correlations are low and generally not significant in both ethnic groups (Table 4.16). Interclass correlations between parents and offspring are also presented in Table 4.16. In the EA sample, stature correlations range from 0.29 to 0.43 (pS0.05). The correlations for stature in the total sample are somewhat lower, and the father-son correlation is not significant. Familial conelations for stature in the FN sample are low and generally not significant except for motherodaughter (r--0.51, p50.05) and mother-offspring (tr-0.29, p50.05) pairs. Patterns of familial correlations indicate significant familial resemblance in all skeletal breadths, circumferences and AMA. Significant correlations vary with sample sizes, as larger correlations are required to reach significance in small samples. This probably explains the large number of non-significant correlations in the FN sample. Estimates of heritability based on mid-parent regression indicate that stature, skeletal breadths, and circumferences are significantly heritable in me EA and total samples, but not in the FN sample (Table 4.17). AMA does not show significant heritability in any group. Estimated heritabilities for stature are 0.68 (p<0.001) in the EA sample and 0.40 (p<0.001) in the total sample. Significant heritabilities range from 0.25 to 0.59 in the total sample for these measurements. 66 Body Mass, Fatness and Relative Fat Distribution Ethnic Differences Table 4.18 presents the results of ANCOVAs for differences in body mass, fatness and fat distribution between EA and FN samples within age and sex groups. There are several significant differences. In the 5-19 year males, the FN sample has significantly higher means for SUM, TRUNK. EXTREMITY, TER, and WHR (p50.05), indicating that they have greater subcutaneous fatness and relatively more truncal or central subcutaneous fat. In males 20-75 years, EXTREMITY, TER and WHR are significantly higher in the FN sample. Other indicators of fatness are also higher in the FN sample; however, they are not statistically significant due to a large amount of variability and small sample sizes in the FN sample. The results suggest that FN adult men have a greater propensity to store subcutaneous fat on the trunk. In females 5-19 years, FN subjects have a significantly higher TER, indicating that they store relatively more fat on the trunk than EA females (p=0.006). Adult FN females differ from EA females in every indicator of fatness and relative fat distribution. Body mass, BMI, SUM, TRUNK, EXTREMITY, TER and WHR are significantly higher in FN females than in EA females 20-75 years (p50.05). The results indicate that FN females 20-75 years have more subcutaneous fatness and a more central pattern of subcutaneous fat distribution than EA females. Sex Differences Table 4.19 presents the results of the ANCOVAs for differences in body mass, fatness, and relative fat distribution between males and females within age and ethnic groups. In the 5-19 year EA sample, females have significantly higher means for EXTREMITY and SUM, indicating that they are storing more subcutaneous fat than males, possibly due to adolescent loss of fatness on the extremities in males. 67 EA males 20-75 years are heavier and have a greater BMI than females; however, females have higher values for SUM, EXTREMITY and TRUNK, indicating greater subcutaneous fatness. Similarly, FN females 20-75 years are lighter than males, and they have higher means for SUM, TRUNK and EXTREMITY (p50.002). In every age and ethnic group, males have significantly higher TERs and WHRs than females. The results indicate that males accumulate proportionally more subcutaneous fat on the trunk than the extremities than females. Familial Resemblance There is significant aggregation of fatness and relative fat distribution within sibships (Table 4.20). In the EA and total samples, significant intraclass sibling correlations are evident for all indicators of fatness and relative fat distribution. Correlations range from 0.40 to 0.57 in the EA sample, and from 0.13 to 0.38 in the total sample. In the FN group, indicators of fatness do not aggregate within sibships; however, indicators of relative fat distribution show significant intraclass correlations, 0.25 for the TER and 0.50 for the WHR (pS0.05). There is also significant familial resemblance in fatness and relative fat distribution between generations (Table 4.21). There does not appear to be assortative mating for body mass, fatness or relative fat distribution in this sample. Spousal correlations are low and not significant for all variables. Significant parent-ofi‘spring correlations range from 0.20 to 0.57. All variables, with the exception of the WHR, demonstrate significant father-offspring and mother-offspring correlations in the EA sample, and with the exception of TRUNK and WHR in the father-offspring correlations, all correlations are significant for father-offspring and mother-offspring in the total sample. Correlations in the FN sample are low and rarely reach significance probably due to small sample sizes. It appears as though there are several spurious negative correlations, particularly in the father-son category which has only 10 pairs of FN subjects. 68 Heritability estimates from regression of offspring on mid-parent values are presented in Table 4.22. The estimates in the FN sample are generally low and none are significant. With the exception of the WHR, all heritability estimates are significant in the EA and total samples (p50.003). Estimates of h2 range from 0.30 to 0.45 in the EA sample and from 0.25 to 0.42 in the total sample. Prevalence of Obesity The prevalence of obesity using the BMI and triceps skinfold as criteria are presented in Table 4.23. Prevalence of obesity using the BMI is higher in FN subjects of all age and sex groups, ranging from 29.4% in FN females 5-19 years to 58.8% in FN females 20-75 years. However, the prevalence of obesity using the BMI is also high in the EA sample, ranging from 16.9% in BA females 5-19 years to 39.0% in EA males 20-75 years. The prevalence of obesity, based on the triceps skinfold, is highest in females 20-75 years. Prevalences of obesity based on the triceps skinfold range from 11.8% in FN females 5-19 years to 47.1% in FN females 20-75 years. The prevalences of obesity based on the triceps alone (TSF Obese), the BMI alone (BMI Obese), and the triceps and BMI together (TSF+BMI Obese). are also presented in Table 4.23. Estimated prevalences of TSF Obesity range fiom 0.0% in FN females 5-19 years to 8.5% in EA females 5-19 years. In contrast, the estimated prevalences of BMI Obesity range from 2.7% in BA males 5-19 years to 40.0% in FN males 20-75 years. Estimated prevalences of TSF+BMI Obesity range from 10.2% in BA females to 43.1% in FN females 20-75 years. Prevalences of obesity differ significantly between EA and FN females 20-75 years. The estimated prevalences are higher in the FN sample (pS0.05) for triceps independently, the BMI independently, and TSF+BMI Obese (Table 4.23). Differences between adult subjects classified as BMI Obese and TSF-t-BMI Obese are presented in Table 4.24. There were insufficient numbers in the 5-19 year old groups to make comparisons. Also, there were too few subjects classified as TSF 69 Obese to compare to the other groups. In all sex and ethnic groups except EA females, BMI Obese subjects have greater TERs than the TSF+BMI Obese (pS0.05). In all groups, except FN males, there were significant somatotype differences between the groups classified as obese by different criteria. In general, endomorphy is greater in the TSF+BMI Obese group, and in the EA sample, mesomorphy is greater and ectomorphy is lower in the TSF+BMT Obese sample. In EA males, the TSF-t-BMI Obese group has a greater mean biepicondylar breadth, while both EA male and female TSF+BMI Obese have greater bicondylar breadths. 70 Physique Ethnic Differences There are significant differences in Heath-Carter anthropometric somatotypes between FN and EA subjects (Table 4.25). Significant MANCOVAs, with age as the covariate, are apparent for males 5-19 years (p=0.002), and 20-75 years (p=0.04), and for females 20-75 years (p<0.001). The MANCOVA for females 5-19 years is not significant (p=0. 10). Results of the univariate somatotype component F-tests for pairwise comparisons are also presented in Table 4.25. In males 5-19 years, FN subjects are significantly more endomorphic than the EA subjects (p=0.004). In males 20—75 years, endomorphy (p=0.03) is significantly greater and ectomorphy (p=0.01) is significantly lower in the FN sample. In 20-75 year old females, endomorphy (p<0.001) and mesomorphy (p=0.001) are significantly greater, and ectomorphy (p<0.001) is significantly less in the FN sample. Although not significant, somatotype differences between EA and FN females 5-19 years are in the same direction as in the other age and sex groups (greater endomorphy). Forward discriminant function analyses indicate that endomorphy is the most important discriminator between FN and EA subjects in all age and sex groups, entering the analysis fust in all groups (Table 4.26). In males 5-19 years and females 20-75 years, ectomorphy enters the analysis as the second most important discriminator, followed by mesomorphy. In males 20-75 years and females 5-19 years, mesomorphy enters second, followed by ectomorphy. Sex Differences Somatotypes of males and females differ in both the EA and FN samples. Table 4.27 presents the results of the overall MANCOVAs for sex differences, which are determined for all age and ethnic groups (pS0.004). Table 4.27 also presents the results of pairwise comparisons for somatotype component differences between males 71 and females. EA males 5- 19 years and 20-75 years are significantly less endomorphic and significantly more mesomorphic than females (p50.003). FN males 5-19 years are significantly more mesomorphic than females (p=0.02), and FN females 20-75 years are significantly more endomorphic than males (p<0.001). The results of forward discriminant function analyses for discriminating between males and females are presented in Table 4.28. In the EA sample, endomorphy is the most important discriminator, followed by mesomorphy and ectomorphy in both age groups. Similar results are evident in the 20-75 year FN sample; however, in the 5-19 year age group, the best discriminator is mesomorphy, followed by endomorphy and ectomorphy. Familial Resemblance Intraclass sibling conelations indicate significant aggregation of somatotype within sibships (Table 4.29). The EA sample demonstrates significant correlations for mesomorphy (rim=0.57, p<0.001) and ectomorphy (rm=0.55, p<0.001), whereas the correlation for endomorphy is not significant (rim=0.07, p=0.374). The total sample follows a similar pattern; rim=0.29 for mesomorphy (p<0.001) and rim=0.27 for ectomorphy (p<0.001), and rm=0.07 for endomorphy (p=0.171). The FN correlations are IOWCI’ in magnitude and not significant. Spousal and parent-offspring interclass correlations for somatotype are presented in Table 4.30. Spousal correlations are low and not significant, indicating no assortative mating for somatotype. Only 8, 2, and 9 of 18 parent-offspring correlations are significant in the EA, FN and total samples, respectively. Significant correlations range from 0.20 to 0.49 across all samples. Correlations in the FN sample are generally of the same magnitude as in the EA sample; however, correlations are not significant due to small samples (9-34 pairs). Significant correlations do not follow an apparent pattern. 72 Heritability estimates based on mid-parent regression indicate significant familial resemblance in somatotype in the EA and total samples (Table 4.31). Heritability estimates in the FN sample are low and not significant, probably due to small sample sizes. For endomorphy, h2=0.27 inthe EA sample (p=0.027) and h’=0.26 in the total sample (p=0.022). Similarly, h2=0.34 in the EA sample (p=0.001) and h2=0.24 in the total sample (p=0.012) for mesomorphy. Heritability estimates for ectomorphy are slightly lower and not significant. 73 Grip Strength, Trunk Flexibility and Motor Fitness Ethnic Differences There are few differences between EA and FN subjects in grip strength, trunk flexibility and motor fitness (Table 4.32). The only age and sex group which shows significant differences is males 20-75 years. Adult EA males are stronger in both right and left grip strength, and are more flexible in the lower trunk than FN males (p50.02). Sex Differences Males and females of European and FN ancestry consistently differ in grip strength and flexibility (Table 4.33). Males in all age and ethnic groups are significantly stronger than females in grip strength (p50.03). On the other hand, EA and FN males 20—75 years are not as flexible as females of the same age (p50.05). Familial Resemblance . Intraclass sibling correlations for strength and flexibility are presented in Table 4.34. With the exception of flexibility in the EA sample, all variables demonstrate significant aggregation within sibships. Sibling correlations for right grip strength are 0.36, 0.30 and 0.28 in the EA, FN and total samples, respectively (p50.02). Similarly, sibling correlations for left grip strength are 0.49, 0.26, and 0.25 in the EA, FN and total samples, respectively (p50.04). Sibling correlations for flexibility are 0.27 and 0.15 in the FN and total samples (p50.03). The correlation is of similar magnitude in the EA sample (rim=0. 16); however, it was not significant (p=0.21). Spousal correlations for grip strength and flexibility are low and not significant (Table 4.35), indicating no assortative mating for these variables. Generally, parent- offspring correlations indicate significant familial resemblance between generations for grip strength and flexibility (Table 4.35). In the total sample, 16 of 18 correlations are significant and range from 0.00 to 0.48. There is no apparent pattern to the correlations; however, due to small sample sizes in the FN group, few correlations are significant. 74 Table 4.36 presents heritability estimates based on regression of offspring on mid-parent values. Grip strength and flexibility are significantly heritable in all samples. Estimates of h2 for right grip are 0.28, 0.62, and 0.34 for the EA, FN and total samples, respectively (pS0.03). For left grip, h2 estimates are 0.29, 0.57 and 0.36 for the EA, FN and total samples respectively (p50.03). Heritabilities for flexibility are 0.49, 0.38 and 0.49 for the EA, FN and total samples, respectively (p<0.001). 75 Interrelationships Among Body Size, Fatness, Physique and Health-Related Fitness Body Size, Fatness and Health-Related Fitness Table 4.37 presents first order partial correlations between indicators of body size and fatness, and motor fitness, controlling for age. In all age groups, body size (stature, mass and the BMI) is positively associated with right and left grip strength; larger people are stronger. SUM is consistently positively related to right and left grip strength. but the association only reaches statistical significance in 20-75 year males and 5-19 year females (p50.05). Correlations between body size, fatness and flexibility are generally negative, and are significant in females 20-75 years. Correlations among body size, fatness and motor fitness in males and females 5-19 years are variable in magnitude and follow few patterns. In males, all indicators of body size and fatness are negatively associated with the flexed arm hang but correlations in females are not consistent. In both sexes, SUM is negatively related to distance covered in the standing long jump (p50.05). Body mass, the BMI and the TER are also negatively related to the standing long jump in females (pS0.05). Correlations for the 35-meter dash show different results for males and females. Indicators of fatness and relative fat distribution are negatively related to time to cover 35 meters, i.e., positively related to performance in males. In females, indicators of fatness and relative fat distribution are significantly positively related to the time to cover 35 meters, i.e., negatively related to performance. Physique and Health-Related Fitness Third-order partial conelations between somatotype components and fitness measures, controlling for age and the other two somatotype components are given in Table 4.38. Significant correlations appear only sporadically in the table, with few patterns apparent. Mesomorphy is positively related to right and left grip strength in all age and sex groups, with correlations ranging from 0.20 to 0.35 (pS0.05). 76 Endomorphy is negatively related to flexibility, demonstrating significant correlations in three of the four age and sex groups. Correlations between endomorphy and flexibility are -0.17 (as) in males 5-19 years, -0.20 (p50.05) in males 20-75 years, —0.37 (p50.05) in females 5-19 years, and -0.30 (pS0.05) in females 20-75 years. Endomorphy is also negatively related to right and left grip strength in females 20-75 years, r=-0.17 and =-0.15, respectively (p50.05). In the 5-19 year group, endomorphy is negatively related to the number of sit- ups performed in 60 seconds. Correlations between endomorphy and sit-ups are -0.31 in males and —0.35 in girls (p50.05). There are no other trends apparent in the analysis of motor fitness. Body size and Familial Resemblance in Grip Strength and Flexibility Table 4.39 presents the results of analyses aimed at investigating the effect of incorporating body size into familial analyses of grip strength and flexibility. Intraclass sibling correlations do not increase once the effects of body size (mass, stature or BMI) are controlled using regression techniques. In the case of flexibility, the intraclass correlations decrease below the level of significance once body size is accounted for. An examination of parent—offspring correlations reveals that incorporating body size into the analyses has little effect on the magnitude of the familial resemblance. Similarly, heritability estimates based on regression of offspring on mid-parent values are not improved by the incorporation of body size in the regression. CHAPTER V DISCUSSION Introduction The results indicated significant differences between Canadians of First Nation (FN) and European (EA) ancestry, and significant familial resemblance in body size and indicators of health-related fitness. This chapter discusses the results in terms of stature and skeletal dimensions; fatness and relative fat distribution; physique; grip strength and flexibility, and the interrelationships among body size, fatness, physique and familial resemblance in health-related fitness. Due to small numbers in the FN sample, familial correlations were generally not significant for most variables. Likewise, differences in familial resemblance between EA and FN could not be determinedduetosmall numbersintheFNgroup,althoughtheredidnotappcartobe any differences in EA and FN correlations. Therefore, the discussion focuses on familial resemblance in the total combined sample of EA and FN. Stature and Skeletal Dimensions Phenotypic Comparisons Stature did not differ among EA and FN subjects within age and sex groups. Also, z-scores for stature indicated no differences between EA and FN subjects relative to reference data. An examination of the distribution of stature by age in males and females did not demonstrate any apparent differences between EA and FN groups; however, the stature of EA males and females increased relative to the reference data in late adolescence into early adulthood. As expected, males of all age and ethnic groups were significantly taller than females. The results are consistent with the findings of 77 78 several studies which indicate that stature of Aboriginal North Americans is not significantly different from the general population (Table 2.1). There were few differences in skeletal dimensions between EA and FN subjects. The exception is the 20-75 year old females who had significantly greater biacromial, bicristal, bicondylar, and biepicondylar breadths than EA females (Table 4.13), indicating that there were ethnic differences in skeletal robusticity. Perhaps the greater adiposity and BMI of the FN females has had an effect on the skeleton. Adult FN females Males generally had greater skeletal breadths than females who had significantly greater HSRs than males of all ages. Additionally, there were no significant sex differences in bicristal breadth, with the exception of EA adults, in whom males had a greater mean than females (Table 4.14). These results are consistent with findings that males have broader shoulders than females, relative to the hips, but absolute hip breadths are not different between the sexes (Malina and Bouchard, 1991). Familial Resemblance Several studies have indicated significant positive assortative mating for stature based on spousal correlations (Tables 2.3 and 2.4). However, spousal correlations were low and not significant in the present study (Table 4.16). Spousal correlations for stature in the CFS were 0.43 (Perusse etal., 1988), while in a sample from Montreal, spousal correlations were 0.25 (Annest et al., 1983). Correlations of a similar magnitude have been reported for samples from the U.S. (Ramirez, 1993; Rotimi and Cooper, 1995, Heller et al., 1984; Malina et al., 1976). The spousal correlation for stature in the present sample (r=-0.01) is similar in magnitude to the value of 0.06 reported for Black Americans from Philadelphia (Malina ct al., 1976). The pattern of correlations among relatives indicates that both genetic and environmental factors are important in explaining familial resemblance in stature. The intraclass sibling correlation for stature is higher (r=0.53) than parent-offspring correlations, which range from 0.16 to 0.37 (Tables 4.15 and 4.16), indicating that the 79 shared family environment is important in the familial aggregation of stature. The estimated heritability based on offspring-midparcnt regression is 0.40, while the parent- offspring correlations are significant (with the exception of father-son), indicating that genetic factors are also probably important. Spousal similarities in skeletal dimensions and circumferences are rarely reported; however, sibling and parent-offspring conelations suggest genetic factors are important in explaining the phenotypic variability in these traits (Tables 2.5 and 2.6). The sibling correlation for sitting height in the present study (r=0.47) is similar to those reported for Belgians (#040; Susanne, 1975), U.S. Whites (0.34 to 0.61; Mueller and Malina, 1980), and U.S. Blacks (0.39 to 0.61; Mueller and Malina, 1980). Similarly, sibling correlations for skeletal breadths fall within the range of those reported in Table 2.6. Parent-offspring correlations for skeletal dimensions and circumferences also fall within the range of values reported in other studies. The magnitude of sibling and parent-offspring correlations are similar, which makes it difficult to speculate about genetic and environmental influences on these traits. 80 Fatness and Relative Fat Distribution Phenotypic Comparisons EA and FN subjects differed significantly in fatness and relative fat distribution phenotypes. Generally, FN subjects were fatter and had a more central fat distribution that EA subjects. The differences between ethnic groups were greatest among adult females; however, there were differences apparent in each age and sex group. Some differences were not statistically significant in all groups, but the direction of the difference was consistent. FN females 20-75 years had a greater mean body mass than EA females of the same age, while body mass did not differ between ethnic groups in the other age and sex groups. A comparison of adult body mass relative to reference data indicated that FN females were heavy throughout adulthood, tracking at the 90th percentile. These results suggest that FN females are at increased risk of overweight and/or obesity during adulthood. Males generally had less subcutaneous adiposity, but had a greater tendency to store proportionally more fat on the trunk than females. These differences are. particularly evident in the adults of both ethnic groups; the trends are also apparent in the younger age groups, especially for relative fat distribution. The finding of greater central fat deposition in males is consistent with observations among the Canadian Inuit and the Siberian nGanasan (Rode and Shephard, 1995). Similarly, Hall et al. (1991) indicated that mean WHRs among the Navajo were 0.90 for females and 0.96 for males 2 20 years of age. Corresponding WHRs in the present study of FN are 0.85 for females and 0.93 for males, indicating a similar relative fat distribution. According to anthropometric z-scores in each age and sex group, EA and FN subjects carried more subcutaneous fat on the trunk versus the extremity relative to reference data (NHANES II; Najjar and Rowland, 1987). In all groups except FN 81 males 5-19 years, anthropometric z-scores for the triceps skinfold were consistently lower than those for the subscapular skinfold in each group. The results are somewhat consistent with the data presented by Johnston ct al. (197 8) for urban Native American school children, which indicated that Native American females tend to carry proportionally more subcutaneous fat on the trunk relative to reference data, while boys do not. In the present sample, both EA and FN groups had higher z-scores for the subscapular skinfold than for the triceps skinfold. The TER was compared to values from the Quebec Family Study (QFS) in Figures 5.1 through 5.4. In EA males and females 5-19 years, mean TERs approximate the medians from the QFS, whereas FN TERs are higher. Among adults, the TERs of EA subjects are higher than the QFS values, and FN TERs are higher still. These comparisons suggest that adults in the present sample have a more central or truncal subcutaneous fat distribution than the Quebec sample. There are little North American reference data available for the WHR. The WHRs of the present sample of adults were compared to a study fi'om France (Tichet ct al., 1993). In general, the WHRs of EA adults approximated the medians of the reference data (Figures 5.5 and 5.6), whereas the WHRs of FN adults were between the 50th and 95th percentiles. In both males and females, FN adults demonstrated greater WHRs than EA adults, and the difference was more apparent in the females. The comparisons of relative fat distribution indices to other studies indicate that the present sample have a proportionally greater amount of subcutaneous fatness on the trunk than on the extremities. Although males have greater TERs and WHRs than women, FN adult females appear to deviate the most from reference values. Familial Resemblance There is significant familial resemblance for all indicators of fatness and relative fat distribution, however, spousal correlations for all variables are low and not significant, ranging from 0.01 to 0.11 (Table 4.21), which suggests that assortative 82 mating for fatness has not occurred, and that the role of the living environment has had only minimal effects on spousal similarities in fatness and relative fat distribution in this population. These results are somewhat consistent with the spousal conelations presented in Table 2.8, which indicate that some populations have significant spousal correlations and some do not. Possible explanations for this finding include a sexual division of labor and changing activity patterns in this population. There appears to be a significant sexual division of labor such that men are occupied in activities which may be more energy expensive (construction work, guiding etc.), whereas females may be involved more in less energy expensive activities (housework, clerical professions). The FN people of Temagami and Bear Island are acculturated and they do not have to rely on traditional lifeways to survive. The acculturation process has probably resulted in changing activity patterns in both men and women, such as has been demonstrated in other populations (Godin and Shephard, 1973). Thus, a sexual division of labor, as well as perhaps differential effects of acculturation on FN men and women may explain the lack of significant spousal correlations for fatness. Intraclass correlations among siblings indicate significant sibship effects. The magnitude of the correlations are similar across the BMI, SUM and TRUNK; 0.28, 0.23, and 0.29, respectively. The sibling correlation is lower for EXTREMITY, 0.13, and higher for TER and WHR, 0.38 and 0.37, respectively. Parent-offspring correlations are generally of similar magnitude as the sibling correlations. Mother-offspring correlations are the strongest and most significant, but this may reflect larger sample sizes for mother—offspring than for father-offspring conelations. Alternatively, the maternal influence on fatness and relative fat distribution may be greater than the paternal influence. A greater maternal influence could operate through genetic or environmental pathways, or both. However, there is no clear evidence from the literature for a specific maternal or paternal effect on fatness (Bouchard et al., 1997). 83 Correlations for all indicators of fatness are of similar magnitude, but TER and WHR demonstrate lower correlations. Similarly, heritability estimates based on offspring mid-parent regressions are higher for fatness indicators than for TER and WHR. The results suggest that both genetic and environmental effects are important for the familial aggregation of body fatness. Relative fat distribution may have a greater influence from the living environment, based on high sibling correlations. The results for relative fat distribution are not consistent with studies in the literature (Bouchard et al., 1997). Using the twin model, Selby et al. (1989) indicated that the level of heritability for central deposition of subcutaneous fat was quite high (0.77). Similarly, the transmissibility of the TER and the WHR across generations was 37% and 28%, respectively, in the CFS (Perussc ct al., 1988). Perhaps greater environmental effects on fatness were operating in the present sample- which increased the familial resemblance. Such an effect might overshadow a possible greater influence from genetic factors on fat distribution. Relative fat distribution is dependent on overall fatness (Garn et al., 1982; Malina, 1996). In the present study, there was a positive association between SUM and the TER and WHR which indicated that relative central subcutaneous fat distribution increased as fatness increased (Table 5.1). With the exception of FN females (rs-0.32, p<.05), correlations between SUM and indices of relative fat distribution were generally positive and significant (0.18 to 0.80). The results in the FN females should be viewed with caution, as this sample is significantly fatter than any other group studied, and interrelationships among fatness indicators may differ in the markedly obese. . Given that there is a relationship between subcutaneous fatness and relative fat distribution (Table 5.1), family correlations for fat distribution (TER, WHR) were recalculated after controlling for subcutaneous fatness (SUM) (Table 5.2). With few exceptions, the recalculated correlations are similar to the original ones. Differences do 34 not follow a pattern. Thus, the use of regression to adjust indicators of relative fat distribution for the effects of fatness does not appear to affect estimates of familial aggregation. The estimate of heritability for body mass is 0.26 based on mid-parent regression, which is lower than that for stature; however, parent-offspring correlations for body mass are higher than for stature, ranging from 0.10 to 0.45. The intraclass sibling correlation for body mass is 0.29, which is lower than the correlation for stature, and generally lower than the parent-offspring correlations for body mass. Thus, it appears as though genetic factors are more important than the living environment in explaining the familial resemblance in body mass. Prevalence of Obesity The prevalence of obesity in FN is generally higher than in EA. Among children and youth 5-19 years, estimated prevalences of obesity (285th percentile age-specific NHANES II BMI; Najjar and Rowland, 1987) are 2.3% in BA males, 38.1% in FN males, 16.9% in BA females, and 29.4% in FN females. These prevalences were greater than those reported by Broussard et al. (1991) among Native American adolescents: 24.5% in males and 25.0% in females (285th percentile age-specific NHANES II BMI; Najjar and Rowland, 1987). Based on comparisons to 95th percentiles of the BMI in the NCHS data set, 11.2% and 12.5% of Navajo girls and boys, respectively, exceeded the cut-off (Sugarman et al., 1990). Prevalences of obesity in FN subjects 5- 19 years based on the triceps skinfold (285th percentile age-specific NHANES II triceps skinfold; Najjar and Rowland, 1987) were 28.6% in males and 11.8% in females. Corresponding estimates in Cherokee youth (285th percentile triceps skinfold, Ten State Nutrition Study) were 49.7% in boys and 31.6% in girls 13-17 years (Story et al., 1986). These statistics are not directly comparable though as the present sample encompasses a wider age range. 85 Significant differences in the prevalence of obesity were evident only in the 20- 75 year old females. In adult females the estimated prevaleirce of obesity based on the BMI (BMI 2 27.8 in males and BMI 2 27.3 in females) was 58.8% in the FN and 35.0% in the EA (p=0.002). Corresponding prevalences in adult males were 51.4% FN and 39.0% BA for the BMI (Table 4.23). Broussard et al. (1991) estimated that prevalences of overweight (BMI 2 27.8 in males and BMI 2 27.3 in females) in Native American adults 2 18 years at 33.7% in males and 40.3% in females, which are lower than those estimated in the present study. The prevalence of overweight among the Navajo (BMI 2 27.8 in males and BMI 2 27.3 in females) was estimated at 30.3% in males and 50.0% in females (Hall et al., 1991), which is similar for females but lower than that for males in the present study. Age specific prevalence rates for overweight (BMI 2 27.8 in males and BM] 2 27 .3 in females) among the Pima ranged from 31% to 78% for males 220 years, and from 48% to 87% for females 2 20 years (Knowler etal., 1991). The estimated prevalence of obesity based on the triceps skinfold (triceps skinfold 2 17.5 mm in males and triceps skinfold 2 29.5 mm in females) was 47.1% in FN and 29.9% in BA females 20-75 years (p=0.03). Corresponding prevalences in males were and 17.1% FN and 14.1% EA. There is considerable variability in the cut-off point used to define overweight/obesity among studies. Estimated prevalences will vary by the percentile cut-off used (ex. 85th, 95th percentile) as well as the reference data used to define the cut-off. Although different criteria were used among studies, the evidence indicates that Native Americans have a greater prevalence of obesity than the general North American population. Studies of the prevalence of overweight among Native groups generally indicate higher prevalences in females than males (Broussard et al., 1991; Hall et al., 1991; Knowlcr et al., 1991; McIntyre and Shah, 1986; Young and Sevenhuysen, 1989). 86 Results of the present study indicate that adult FN males have a similar rate of obesity as females, 51.4% and 58.8%, respectively. This trend is also evident in EA adults. Males have a rate of 39.0% and the females have a rate of 35.0%. Females 5-19 years demonstrate lower prevalences of obesity than males of the same ethnic group. The results suggest that adult males in the present sample are heavy for their stature, but they are not overly fat, as BMI Obese rates are more than double triceps obese rates, and TSF+BMI Obese rates are 11.4% FN and 13.0% EA (Table 4.23). In contrast, adult females are both heavy and fat, as indicated by high prevalences of BMI Obesity and TSF Obesity, with TSF+BMI Obesity rates of 43.1% FN and 26.0% EA. Using the classification scheme of Van Italic and Abraham (1985), prevalence rates of obesity differ by the criteria used, i.e., BMI, triceps skinfold, or both (Table 4.23). There are also morphological differences between adult subjects classified as obese by the different criteria (Table 4.24). In all sex and ethnic groups except FN males 20-75 years, BMI Obese subjects have greater TERs than TSF+BMI Obese (p50.05). In general, endomorphy was also greater in the TSF+BMI Obese group, and in the EA sample, mesomorphy was greater and ectomorphy was lower in the TSF+BMI Obese sample. In EA males and females, the TSF+BMI Obese group also demonstrated greater bicondylar breadths. Comparisons between TSF Obese and BMI Obese may not be equivalent for FN and EA groups. Given that FN has a significantly greater truncal subcutaneous fat distribution, the use of the triceps skinfold to assess obesity may not be valid, and may underestimate the prevalence. Likewise, the use of the subscapular skinfold may overestimate the prevalence of obesity in FN groups. A combination of triceps+subscapular may be the best alternative. A similar question can be raised when comparing obesity rates between men and women because men have a greater truncal subcutaneous fat distribution than women. 87 A study among U.S. school children from Philadelphia also demonstrated differences among subjects classified as obese by similar criteria as used in the present study (Malina et al., 1989). The TSF+BMI Obese children were heavier and taller, had greater alrn muscle circumferences, and had greater bicondylar and biepicondylar breadths than the TSF obese group. The characteristics of the TSF+BMI Obese EA samples in the present study had greater bicondylar and biepicondylar breadths than the BMI obese group, but comparisons to a TSF obese group could not be made due to small numbers. Physique Phenotypic Comparisons The results indicated that FN subjects were significantly more endomorphic than EA subjects in all groups except 5-19 year old females, in whom differences in somatotype were small but in the same direction as the other groups (greater endomorphy). Females were consistently more endomorphic than males within age groups; however, the somatotype difference was not significant in the FN 5-19 years. Also, males were significantly more mesomorphic in all age and ethnic groups, except FN adults, where the difference was small and did not reach significance. Thus, FN females were the most endomorphic in the present study. The results are consistent with a study of Alaskan Eskimos which demonstrated that Eskimo men and women had a physique characterized by high endomorphy and mesomorphy (Carter and Heath, 1990). Mean adult somatotypes in Eskimos were 3.4- 5.9—1.3 in males and 6.4-4.8-0.8 in females. These data suggest that females are more endomorphic than males, and that males are more mesomorphic than females. Mean adult somatotype in this study was 5.2-6.2-1.0 in FN males and 7.4-5.9-0.7 in FN females. Corresponding values for EA subjects were 4.6-6.014 for males and 6.1- 5.1-1.3 for females. There are three major sources of comparative somatotype data for Canadians: the YMCA-LIFE program (Bailey, 1982), the Canada Fitness Survey ( CFS, Perusse et al., 1988), and the Quebec Family Study (Katzmarzyk et al., 1997; Malina ct al., 1997). The YMCA-IJFE program was a nation-wide testing program conducted in 1976—78 to characterize the lifestyle and fitness of Canadians (Bailey etal., 1982). A large sample (13,599 subjects) of Canadians were somatotypcd by the Heath-Carter anthropometric protocol as part of the YMCA-LIFE program The CFS was conducted in 1981 and involved collecting anthropometric and fitness data on 13, 804 subjects 7 to 69 years of age from across Canada (Pérusse et al., 1988). The anthropometric 89 battery of the CFS included the dimensions necessary for the calculation of Heath- Carter anthropometric somatotypes. Phase I of the Quebec Family Study (QFS) was conducted from 1978-82, which involved collecting anthropometric, activity, dietary, fitness, and metabolic data on a sample of French Canadian subjects from the Greater Quebec City area (Bouchard, 1989). Heath-Carter anthropometric somatotype was assessed as part of the anthropometric battery of the QFS, and the data used in the analyses of Katzmarzyk et al. (1997) and Malina et al (1997) were reanalyzed according to the age groups used in the present study for the purpose of providing comparative data. Little data have been presented on the somatotypes calculated in the CFS. Perusse ct al. (1988) present mean somatotypes for the entire CFS sample, from 7 to 69 years of age. The mean somatotypes were 3.6-4.9-2.2 for males and 4.4-4.2—2.1 for females (Perussc et al., 1988). These results compare to mean somatotypes of 4.2- 5.6-1.8 and 5.0-5.7-1.5 for EA and FN males, respectively, and 5.7-4.7-1.7 and 6.7- 5.3—1.3 for EA and FN females, respectively, from 7 to 69 years in the present sample. The EA sample in the present study is more endomorphic and mesomorphic, and slightly less ectomorphic than the CFS sample. The FN sample is considerably more endomorphic and mesomorphic, and less ectomorphic than the CFS sample. Table 5.3 presents mean Heath-Carter anthropometric somatotypes for this study, the YMCA-LIFE program (Bailey et al., 1982), and the QFS (Katzmarzyk et al., 1997; Malina et al., 1997) by age and sex. The QFS group is less endomorphic and more ectomorphic than the other studies in the 15-19 and 20-29 year age groups; thereafter the QFS means are similar to those for the YMCA-LIFE program The FN sample is consistently more endomorphic and mesomorphic, and less ectomorphic than the other samples, especially in the older age groups. Similarly, the EA group tends to approximate the means of the YMCA-LIFE program in the 15—19 and 20-29 year age 90 groups; thereafter, the EA sample consistently demonstrates higher endomorphy and mesomorphy than the other samples, with the exception of the FN. Familial Resemblance Spousal correlations for somatotype are uniformly low and not significant, demonstrating correlations of 0.14 for endomorphy, 0.08 for mesomorphy, and 0.02 for ectomorphy. These are comparable to the spousal correlations of 0.14, 0.10, and 0.12 for endomorphy, mesomorphy, and ectomorphy, respectively, in the CFS (Pénrssc etal., 1988). Low spousal correlations have also been demonstrated in the QFS: 0.05, 0.10, and 0.06 for endomorphy, mesomorphy and ectomorphy, respectively (Song et al., 1993). Corresponding spousal correlations based on maximum likelihood estimations in a sample from Spain were 0.19, -0.08, and 0.14 for endomorphy, mesomorphy, and ectomorphy, respectively (Sénchez-Andres, 1995): Thus, the available evidence suggests that assortative mating for physique, as assessed by the Heath-Carter anthropometric somatotype, is quite small; however, there may be differences among other cultures. Parent-offspring and sibling correlations are generally higher than spousal correlations indicating significant familial resemblance in somatotype. Intraclass correlations within sibships are 0.29 for mesomorphy and 0.27 for ectomorphy, whereas the correlation for endomorphy was low and not significant. Resemblance between fathers and sons, based on interclass correlations, was quite low, whereas mother-daughter correlations for all three somatotype components were significant. Correlations among fathers and offspring are low and not significant for endomorphy, while those among mothers and offspring are significant, indicating that there may be a maternal effect in the transmission of endomorphy between generations. Since endomorphy generally indicates a preponderance of fatness, the maternal effect could be explained by cohabitation and the mother’s role in providing nutrition for her Children, rather than a maternal genetic effect. 91 Parent-offspring correlations in the present study are similar in magnitude to those reported in the literature (Table 2.9). Parent-offspring correlations in the present study ranged from 0.00 to 0.45, which compares to ranges of -0.04 to 0.30 in a Spanish population (Sénchez-Andrés, 1995), 0.15 to 0.41 in the QFS (Song et al., 1993), and 0.21 to 0.24 in the CFS (Perusse ct al., 1988). Heritability estimates, based on mid-parent regression, indicate that 18% to 26% of the phenotypic variance in somatotype is explained by familial factors. Using a path analysis, Perusse et al. ( 1988) indicated that the transmissibility from parents to offspring (cultural and genetic factors) accounted for between 36% to 45% of the variance in somatotype in the CFS. The pattern of familial correlations and regressions of offspring on the mid- parent values indicate that mesomorphy demonstrates the most consistent pattern of familial resemblance. Parent-offspring correlations for mesomorphy ranged from 0.15 to 0.22, and the intraclass sibling correlation is 0.29, while the regression coefficient for the offspring-midparent regression was 0.24. The other somatotype components did not show a consistent pattern of association among relatives. These results are consistent with those of Song et a1. (1993), which also demonstrated that the familial aggregation for mesomorphy was the most consistent and strongest of the three components. Similarly, sanchcz-Andrés ( 1995) demonstrated that parent-offspring correlations for mesomorphy tended to be greater than for endomorphy or ectomorphy. Thus, available evidence suggests that familial aggregation for mesomorphy may be greater than for the other somatotype components; however, the results from the studies surveyed cannot separate genetic from environmental effects. 92 Grip Strength, Trunk Flexibility and Motor Fitness Phenotypic Comparisons The distributions of trunk flexibility and combined grip strength were generally between the 10th and 90th percentiles of the reference data in all age and sex poups (Fitness Canada, 1985, 1986). Ten-year age-specific means for combined grip strength (right + left) in BA males 20-75 years were peatcr than the 50th percentile of the reference data. Similarly, EA males 20-75 years are significantly stronger than FN males. All other ethnic comparisons in performance were not significant, with the exception of males 20-75 years, in which EA males demonstrated greater trunk flexibility than FN males. EA and FN children 5-15 years did not differ significantly in sit-ups, flexed arm hang, 35-mcter dash, and standing long jump. The distributions of performance scores generally were between the 10th and 50th percentiles of the reference data for both ethnic groups (Fitness Canada, 1985; Haubenstricker et al., 1991), indicating that the EA and FN children were not performing as well in these events as children in the Canada Fitness Survey (sit-ups) and the Michigan State University MotOr Performance Study (flexed arm hang, dash, and standing long jump). Motor skills are not taught as part of physical education of the Temagami children which may explain some of the observed differences between this sample and the reference data. Given the associations between body size and motor fitness (Malina, 1975, 1994), body morphology in the present sample may help explain the apparent differences between the EA and FN children and the reference data. There is a positive association between body size and grip strength (Table 4.37). Since the body size (stature, mass, BMI) of children in this sample is similar to the children in the Canadian Fitness Survey (see descriptive results), it seems appropriate that the combined pip strength is also similar, although there may be dynamometer differences between the present study and the CFS (Figures 4.69 through 4.72). There is generally a negative 93 association between fatness and performance in events which require the subject to move the body through space, such as in the dash or standing long jump, or to support their body mass, as in the flexed arm hang (Malina, 1994). Triceps and subscapular skinfolds were consistently peatcr than reference data for US. children (N ajjar and Rowland, 1987), demonstrating anthropometric z-scores ranging from 0.08 to 0.80 for triceps and 0.39 to 1.47 for subscapular (Table 4.10). Perhaps the peatcr adiposity of the sample may lead to poorer performance in sit-ups, flexed arm hang, standing long jump, and the 35-meter dash. These associations are explored in the subsequent section on body size, fatness, physique and motor fitness. Familial Resemblance There was significant agpegation of pip strength and flexibility within families. Spousal correlations approximated zero, indicating no assortative mating for these variables. Spousal correlations for grip strength and flexibility in selected studies are also low (Table 2.10), with the exception of studies from Czechoslovakia (Kovar, 1981) and Poland (Szopa, 1982), which demonstrate spousal correlations of 0.26, and 0.15 to 0.26, respectively, for measures of grip strength. Malina ct al. (1983) also reported significant spousal correlations for right grip (r=0.29) and left pip (r=0.27) in a rural Zapotec community; however, second order partial correlations controlling for the ages of husband and wife were not significant (right pip m—O. 12, left grip r=- 0.04). . Sibling conelations for pip strength were within the range of correlations reported in other studies (Table 2.10), and the sibling correlation for flexibility (0.15) was lower than those reported in a Mennonite community (0.44, Devor and Crawford, 1984) and,in the CFS (0.36, Perusse et al., 1988). Parent-offspring correlations for grip strength and flexibility were generally significant, and were of similar magnitude to those reported in selected studies across all variables. 94 Heritability estimates based on the regression of offspring on mid-parent values were 0.34, 0.36, and 0.49 for right grip, left grip, and flexibility, respectively. These values compare well with transmissibility estimates of 0.37 for pip strength/body mass and 0.48 for flexibility in the CFS (Pérussc et al., 1988). Secular Trends Stature There were significant decreases in stature with age in EA males and females, but not in the FN group. Statural loss due to aging (shrinkage) was estimated at 0.12 cm/year and 0.06 cm/year in BA males and females, respectively. These estimates are peatcr than those obtained in Colombian women, which were 0.024 cmldecade in a lower socioeconomic status (SES) group and 0.013 cmldecade in an upper SES poup . (Dufour et al., 1994). The estimated statural loss due to shrinkage in rural Colombian women was 0.027 cm/year, whereas that for men was 0.121 cm/year (Himes and Mueller, 1977a, 1977b). There was also an association between SES and age-related statural loss in the rural Colombian sample, such that individuals from higher SES lost stature at a slower rate than those from lower SES (Himes and Mueller, 1977b). Trotter and Glcscr (1951) estimated an average rate of decline in stature with age of 0.06 cmldecade and suggested that it may be applicable to the general population. The shrinkage effect in EA females was the same as reported by Trotter and Gleser; however, the EA males lost stature at twice this rate. The analysis of secular change in stature indicated that within this sample of Canadians, there was an estimated secular increase of 1.0 cmldecade (p<0.05) and 0.4 cmldecade (us) in BA males and females, respectively. Shephard (1986) reviewed several studies conducted in Canada from 1953 to 1981, and suggested that a secular trend of approximately 1.0 cmldecade in both males and. females has occurred in Canada over the past 25 years; however, there may have been some regional variation (Shephard, 1986). The data also suggested that urban centers in Canada may have 95 experienced a lesser secular gain than rural areas. It must be noted that statistical analyses of the data were not performed, and conclusions were based on examining trends in means among studies. The results of the present study were compared of data collected in 1953 (Pett and Ogilvie, 1956), 1970-72 (Health and Welfare Canada, 1980), and 1981 (Fitness Canada, 1983). Figure 5.7 presents the mean statures of Canadian males 5—19 years from four studies conducted since 1953. It is apparent that stature has increased from 1953 to the present; however, stature in the present study is similar to the 1981 Canada Fitness Survey, with the exception of late adolescence, where the present sample is taller. Figure 5.8 presents the results of four studies of adult males conducted since 1953. There is thus a secular increase in stature over time. Stature among Canadian females folloWs a similar trend as in males. Stature increases with time among the various studies (Figures 5.9 and 5.10). Among females 5-19 years, the present sample is similar in stature to the 1981 Canada Fitness Survey (Fitness Canada, 1983), allowing for sampling variation. Adult female stature is , peatcr in this study than in any previous Canadian study. Body Mass Since body mass has a tendency to increase with age, an internal statistical analysis of secular changes in body mass was not possible. Age specific means for body mass in males 5-19 years are presented in Figure 5.11 with corresponding values from Canadian surveys conducted in 1953 (Pctt and Ogilvie, 1956), 1970-72 (Health and Welfare Canada, 1980), and 1981 (Fitness Canada, 1983). In general, body mass has increased from 1953 to the present; however, the age-specific means of the present study are similar to the 1981 CFS, with the exception of the older age poups, which are heavier (Fitness Canada, 1983). This trend is similar to that observed for stature in the same samples. Among adult males, the current sample is heavier than observed in 96 any study in Canada since 1953 (Figure 5.12). Body mass has generally increased with time in Canada. Body mass of females follows the same trend as in males (Figures 5.13 and 5.14). There has been an increase in the mean body mass of Canadian women since 1953. There is considerable overlap among studies, and the increase does not appear to be as peat as in Canadian males. However, the mean body masses of adult females in the present study are consistently higher than those presented in any previous study. In a review of body mass among selected studies from 1953 to 1981, Shephard (1986) indicated that there had been an increase in body mass among Canadian males and females. Sampling problems and changes in lifestyle (smoking) over the past 35 years made it hard to interpret changes in body mass relative to stature; however, it was noted that the increase in body mass was not peatcr than what would be expected due to the increase in stature which had been observed. Although increases in body mass are apparent with time, this does not mean that there is increasing obesity in the population, as stature has also increased over time. The question which must be addressed is whether stature and body mass have increased in a complimentary manner, or whether body mass has increased proportionally more. To answer this question, secular trends in the BMI were examined. BMI Given recent concern over increasing obesity in the North American population (NIH, 1985), the BMI was estimated from mean statures and body masses in studies from 1953 and 1970-72, and the derived BMI from 1981, and were compared to the BMI in the present study. Figures 5.15 and 5.16 illustrate the BMI in males 5-19 years and 20—75 years, respectively. The trend over time is difficult to interpret from these figures as the distributions overlap considerably. In adult males (Figure 5.16), the 97 present sample has considerably higher BMIs than previous studies, but the previous studies overlap considerably. The BMI of females hour the studies is presented in Figures 5.17 and 5.18. As in males, there is considerable overlap in the distributions of the various studies, and there is no apparent trend over time. . Among adult females, the present sample demonstrates higher BMIs than in any of the previous studies, especially in young adults. The temporal comparisons of the BMI demonstrate that, although body mass has increased in Canada since 1953, it has apparently increased proportionally with the increase in stature. Overall, body size of Canadians has increased from 1953 to 1981; however this increase has not resulted in an increase in the BMI. The exception to this trend is the significant increase in the BMI in the present sample of adults over any previous study. There are two possible explanations for this finding: (1) the population of Temagami demonstrates siprificantly higher values for the BMI than the general population ofCanada,or(2) therehasbeenasignificantincreascintheBhflof Canadians over the past 15 years (1981 to 1996), and the population of Temagami is representative of the rest of Canada. It is difficult to extrapolate these findings to the national level, due to regional differences in body size among Canadians (Shephard, 1986). It is likely that a combination of both explanations could explain the higher BMlsinTemagami, suchthattheremay havebcenanoverall increaseintheBMIin Canada over the past 15 years, and the population of Northern Ontario demonstrates peatcr BMI values relative to Canadians in general. 98 Body Size, Fatness, Physique and Health-Related Fitness Body Size and Health-Related Fitness The results of this study are consistent with established relationships among stature, body mass and health-related fitness (Malina 1975, 1994). In all age and sex poups, stature and body mass were positively associated with right and left grip strength, which has been demonstrated in numerous studies spanning the ages of early childhood through adulthood (Malina, 1975). Greater stature is generally associated with peatcr strength. Partial correlations between stature and right and left pip strength were 0.52 and 0.47 in males 5-19 years, respectively, and 0.59 and 0.69 in females 5-19 years, respectively, controlling for age. Among Philadelphia children 6-11 years, age-specific conelations between stature and right pip strength range from 0.26 to 0.76 in boys and 0.01 to 0.75 in girls (Malina, 1994). Corresponding correlations for left grip strength range from 0.22 to 0.82 and from —0.23 to 0.54 in boys and girls 6-1 1 years, respectively (Malina, 1994). Correlations between stature and right and left pip strength were somewhat lower in a sample of 4-5 year old children (sexes combined), ranging from 0.12 to 0.46 (Merrctt, 1992). Correlations between body mass and pip strength are similar to those reported for stature. Among males and females 5-19 years, partial correlations between body mass and right pip strength, controlling for age were 0.53 in males and 0.58 in females. Corresponding partial correlations for left pip strength are 0.51 and 0.67 in males and females, respectively. Among Philadelphia schoolchildren, age-specific correlations between body mass and right pip strength ranged from 0.34 to 0.79 in boys and from 0.16 to 0.77 in girls (Malina, 1994). Similarly, correlations between body mass and left grip strength ranged from 0.24 to 0.91 in boys and from 0.06 to 0.76 in girls (Malina, 1994). Corresponding correlations among 4-5 year old children 99 (sexes combined) ranged from 0.05 to 0.29 for right and left grip strength (Merrctt, 1992). Partial correlations between stature and body mass, and flexibility, controlling for age followed no consistent pattern, ranging from -0.22 to 0.00 in the present study. There are few comparative data available relating body size to trunk flexibility (Malina, 1994). In boys 5- 19 years, correlations between trunk flexibility and stature and mass were -0.22 (P<0.05) and -0. 16 (ns) respectively. The results are consistent with those of Montoye et al. (1972), who reported age-specific correlations ranging from -0.17 to 0.18 for stature, body mass and trunk flexibility in boys 9-18 years. In girls, correlations were -0.05 (ns) and -0.09 (ns) between flexibility and stature and mass, respectively. Corresponding age-specific correlations for girls 9-18 years ranged from -0.19 to 0.05 (Montoye etal., 1972). | Relationships between body size and sit-ups were low. In boys, correlations between sit-ups and stature and mass were —0.12 and -0. 14, respectively. Corresponding age-specific values for sit-ups were —0.04 to 0.06 for stature and -0. 13 to —0.05 for mass in boys 10-17 years (Espenschade, 1963). Similarly, Montoye et al (1972) reported age-specific correlations ranging from -0.09 to 0.23 for stature and - 0.30 to 0.02 for mass in boys 9-18 years. Correlations between body size and sit-ups were low and positive in girls: 0.18 for stature and 0.16 for mass. Espenschade (1963) reported age-specific correlations of —0.09 to 0.07 for stature and -0. 18 to 0.10 for mass in girls 10-17 years, while Montoye et al. (1972) indicated age-specific correlations ranging from -0.18 to 0.04 for stature and -0.33 to -0.08 for mass in girls 9-18 years. Thee is considerable variability in reported correlations between body size and the dash (Espenschade, 1963; Rarick and Oyster, 1964; Montoye ct al., 1972; Malina, 1975, 1994). In the present study, correlations between stature and the dash were 0.20 (us) in boys and 0.16 (ns) in girls. Corresponding correlations for mass were 0.21 100 (ns) in boys and -0.33 (p<.05) in girls. In 8 year old boys, Rarick and Oyster (1964) reported correlations of 0.19 and 0.07 between the 30-yard dash and stature and mass, respectively. Age specific correlations between the 50-yard dash and stature ranged from ~0.35 to 0.18 in 10-17 year old boys (Espenschade, 1963) and -0.41 to 0.01 in 9- 18 year old boys (Montoye et al., 1972). Corresponding correlations in boys for mass were -0. 14 to 0.30 and -0.11 to 0.26, respectively. Among girls, a similar pattern is evident. Age-specific correlations ranged from -0. 13 to 0.02 for stature and 0.04 to 0.24 for mass in 10-17 year olds (Espenschade, 1964), and from -0.26 to 0.08 for stature and 0.09 to 0.45 for mass in 9-18 year olds (Montoye et al., 1972). It must be noted that neither Montoye ct al. (197 2) nor Espenschade (1963) indicated whether the correlation for the dash had been inverted as in the present study. Age-specific correlations between body size and performance in the 35-yard dash ranged from moderately negative to moderately positive in a sample of Philadelphia school children (Malina, 1994). Correlations in boys from -0.33 to 0.34 for stature and -0.56 to 0.21 for mass. Similarly, correlations in girls ranged from - 0.28 to 0.60 for stature and -0.32 to 0.68 for mass. Correlations between body size and the flexed alrn hang differ by sex in the present study. In boys, correlations between the flexed arm hang and stature and mass were -0.32 (p<0.05) and -0.42 (p<0.05) for stature and mass, respectively. The corresponding correlations for girls were 0.30 and -0.07 for stature and mass, respectively. Comparative data for the flexed arm hang are limited. Montoye et a1. (1972) reported age-specific correlations ranging from -0.26 to 0.08 for stature and - 0.47 to -0.35 for mass in girls 9-18 years. Although not directly comparable, Espenschade (1963) reported age-specific correlations ranging from -0.24 to 0.01 for stature and -0.35 to -0. 10 for mass and number of pull-ups in boys. The available evidence suggests that there is generally a negative relationship between body size and performance in the flexed arm hang, particularly in boys. 101 The standing long jump demonstrates low correlations with body size. Stature was positively related to performance in boys (r=0.25, ns) and negatively related to performance in girls (r=-O.12, ns). Similarly, mass was positively related to performance in boys (r=0.13, ns) and negatively related to performance in girls (m- 0.44, P<0.05). Among Philadelphia school children (Malina, 1994), age-specific correlations between the standing long jump and stature ranged hour 027 to 0.41 and -0. 12 to 0.57 in boys and girls, respectively. Corresponding correlations for mass ranged from -0.39 to 0.39 and —0.34 to 0.41 in boys and girls, respectively. Espenschade (1963) showed positive correlations between stature and the standing long jump, ranging from 0.04 to 0.34 and 0.05 to 0.22 in boys and girls, respectively. Age specific correlations between mass and the standing long jump ranged from -0. 13 to 0.14 in boys and -0.22 to -0.03 in girls. Similarly, age-specific correlations between stature and the standing long jump were generally positive in 9-18 year old children (Montoye et al., 1972). Correlations ranged from -0.02 to 0.42 in boys and -0.02 to 0.34 in girls. Correlations were more negative for mass, ranging fi'om -0.30 to 0.22 in boysand -0.35 to 0.02 in girls. The low-to-moderatc correlations suggest that there is a relationship between body size and motor fitness; however, the relationships vary by sex and age. In general, body mass is negatively associated with performance in events in which the body is propelled through space, and positively associated with strength (Malina, 1994). Fatness, Relative Fat Distribution and Health-Related Fitness In all age and sex poups, there are significant associations between fatness, relative fat distribution and performance. Partial correlations between the BMI, SUM, and grip strength, controlling for age, are consistently positive, although they are of lower magnitude than the correlations between stature, mass and pip strength. Correlations ranged from 0.26 to 0.43 for the BMI and grip strength and from 0.11 to 102 0.28 for SUM. The results are consistent with those among Philadelphia schoolchildren 6-11 years, which indicated that the sum of three skinfolds were generally positively related to right and left grip strength (range -0.21 to 0.72; Malina, 1994). The results suggest that fatness per se does not negatively influence strength; the positive correlations reflect the larger size of fatter children (Malina ct al., 1989). Fatness was weakly and negatively associated with trunk flexibility. Correlations ranged from -0.16 to -0.05 for the BMI, and from «0.26 to -0.12 for SUM. The results are consistent with those reported for Belgian males 12-20 years, which demonstrated correlations between 0.00 and -0. 13 for the sum of four skinfolds and trunk flexibility (Beunen et al., 1983). The associations among fatness and motor performances in males and ferrules 5-15 years were variable in mapritudc, and followed few apparent patterns. SUM was negatively related to the standing long jump in both males and females, with partial correlations, controlling for age of -0.32 and -0.66, respectively. The BMI was also negatively related to the standing long jump in females (@4147), but not in males (1:002). Since the BMI does not distinguish between lean and fat tissue, it may not be representing the same thing in male and female children. The adolescent powth spurt in mass is characterized by increases in muscle mass in boys moreso than in girls (Malina and Bouchard, 1991); thus, the negative association between the BMI and the long jump in girls may be due to increased fatness with a higher BMI in girls but not boys. This explanation is also suggested by the negative association between SUM and the standing long jump in both boys and girls. Similar associations between the sum of three skinfolds and the standing long jump have been reported in Philadelphia children 6-11 years (Malina, 1994). Age-specific correlations ranged from -0.61 to -0.05 in boys and girls (Malina, 1994). SUM was also negatively related to sit-ups in males and females, although the correlations were not significant. Similarly, SUM was negatively related to the flexed arm hang in males (r=-0.43) and females (r=-0.21), 103 which is consistent with results in Belgian males 12-20 years, in whom the sum of four skinfolds had correlations of —0.44 to -0.28 with the flexed arm hang (Beunen ct al., 1 983). Results relating indicators of fatness to the 35-metcr dash are puzzling. In males, there was a positive association between fatness, central fat distribution, and performance in the dash. In females, there was a negative association between fatness and central fat distribution and the dash (Table 4.37). Results of other studies generally indicate a negative association between fatness and the dash or shuttle run (Malina, 1975, 1994). In events which require the subject to propel the body through space (standing long jump, dash) or support the body (flexed arm hang), there is a negative association with fatness (Malina, 1994). Additionally, some evidence suggests that'a more central distribution of subcutaneous fat may have a negative effect on the motor performances of children (Malina and Pena Reyes, 1994). The results of the present study generally fit this suggestion, taking into account a few spurious correlations. Physique and Health-Related Fitness The results of this study generally indicate that endomorphy is negatively associated with fitness and mesomorphy is positively related with fitness, while ectomorphy is not related to fitness. The results are consistent with those from other studies, although not completely comparable, as many studies report zero order correlations within narrow age ranges, and do not control for the other two somatotype components (Malina, 1975). Partial correlations between endomorphy and pip strength ranged from -0. 17 to -0.08, and from -0.37 to -0.17 for flexibility, controlling for age and the other two somatotype components. In a longitudinal study of boys 12-17 years, Clarke (1971) reported correlations ranging from 0.07 to 0.21 between endomorphy and a composite strength score. Generally, correlations between endomorphy and strength are low and 104 positive (Malina, 1975). There was also a negative association between endomorphy and sit-ups in males and females 5- 19 years, controlling for age and the other two somatotype components (—0.31 and -0.35, respectively). Partial correlations between endomorphy and other performance measures showed no consistent pattern. Mesomorphy was positively related to grip strength (r=0.20 to 0.35) and showed no relationship with flexibility. Similarly, Clarke (1971) demonstrated positive correlations ranging from 0.27 to 0.40 between mesomorphy and a composite strength measure in boys 12-17 years. Mesomorphy was not consistently related to other performance measures in the Medford Boys Study (Clarke, 1971). Ectomorphy was not related to strength, flexibility or motor fitness in this study. Generally, correlations between ectomorphy and motor performance variables are low and variable in direction (Malina, 1975). Ectomorphy did, however, demonstrate negative associations with strength, indicating that high ectomorphy was related to a deficit in strength (Malina, 1975). Ectomorphy was generally quite low in the present sample, which could explain the absence of associations with strength and performance. Body size and Familial Resemblance in Strength and Flexibility The results of this study indicate that including measures of body size in correlation and repession analyses does not appreciably alter estimates of familial resemblance (Table 4.39). Sibling correlations were virtually unchanged while heritability estimates from repessions on mid-parent values varied somewhat for pip strength. Table 3.11 presents the amount of variation explained by age-r-agc’+agc3 as well as that explained by adding mass, stature, and the BMI into the repessions independently. In each instance, the incorporation of body size into the repession increased the amount of variance explained; however, the increase was not very peat, since age explained a considerable amount of the variability, particularly in the 5-19 year poups as would be expected. Given that the pr0portion of the total phenotypic 105 variance in performance measures explained using multiple repession did not increase appreciably by incorporating body size into the repession it does not seem surprising that the familial correlations did not change (Table 3.12). The results are not consistent with those of a study in which stature and body mass were partialled out of correlations for performance measures between siblings (Malina and Mueller, 1981). Sibling correlations for strength and motor performance were reduced slightly when body size was controlled by partial correlation, suggesting that removing body size removes some of the covariation due to environmental factors (Malina and Mueller, 1981). However, the results are not directly comparable since different analytical strategies were used in adjusting the variables for the effects of age and sex, and interclass conelations were used to estimate sibling effects rather than intraclass correlation, which was used in the present study. Grip strength is sometimes expressed as a ratio with body mass, i.e., kg/kg body mass. Pérusse ct al. (1988) indicated a parent-offspring correlation of 0.20 and a sibling correlation of 0.29 for grip strength/kg body mass in the CFS. Similarly, Perusse et al. ( 1987) reported a parent-child correlation of 0.32 and a sibling correlation of 0.28 for the same measure in the QFS. These values fall within the range of reported values for unadjusted grip strength (Table 2.10). Grip strength was expressed as right pip, left pip, and relative pip in a study of Polish families (Szopa, 1982). Relative pip strength was calculated a (right+left)/body mass. Familial correlations for left pip and right pip were of similar mapritude as relative pip strength. The variability among correlations for right and left was as peat as between relative pip strength and either right or left. Thus, as in the present study, adjusting pip strength for body mass did little to alter the family correlations. Adjusting performance measures for body size may impact inferences regarding sources of variation in a given trait; however, familial correlations may not be sensitive 106 enough to demonstrate siplificant differences. Additionally, measurement variability will increase the error in the familial correlations. A study which incorporates low measurement error and large numbers of subjects may be needed to estimate the effects of incorporating body size into the estimation of familial effects on performance. CHAPTER VI SUMMARY AND CONCLUSIONS Summary The purpose of the study was to compare Canadians of First Nation (FN) and European (EA) ancestry in terms of body size, physique, and indicators of health- relatcd fitness, and to determine the familial resemblance in these variables. Data were collected during the Spring and Summer of 1996 (May-August) in the Northern Ontario communities of Temagami and Bear Island. All residents 5-75 years of age were eligible to participate. A total of 624 subjects (130 FN, 494 EA) participated in the study. Nineteen anthropometric dimensions were taken on each subject: stature; sitting height; body mass; skinfolds at the biceps, triceps, subscapular, abdominal, suprailiac, supraspinale and medial calf sites; biacromial, bicristal, biepicondylar, and bicondylar breadths; and flexed and relaxed mid-amt, maximal calf, waist and hip circumferences. Eight indices were derived: subischial length; the body mass index (BMI); Heath-Carter anthropometric somatotype; the sitting height/stature ratio; hip-to-shoulder breadth ratio; sum of skinfolds; a trunk to extremity skinfold ratio; and the waist-to-hip circumference ratio. Grip strength and trunk flexrhility (sit-and-reach) were also measured as . components of health-related fitness. Additionally, children attending the public schools (5-15 years) completed a battery of motor fitness tests which included the standing long jump, flexed arm hang, sit-ups, and the 35-meter dash. Technical errors of measurement for the anthropometry were similar to those reported for national Surveys in the United States (Johnston et al., 1972; Malina et al., 1973; Chumlea et al., 107 108 1990; Malina, 1995). Reliability coefficients for the fitness tests exceeded 0.99 for all tests. The results indicated significant differences between EA and FN Canadians and significant familial resemblance in body size and health-related fitness. Generally, FN subjects were fatter and had a more central or truncal subcutaneous fat distribution than EA subjects. The differences between ethnic poups were peatest in the 20-75 year sample of females; however, there were differences apparent in each age and sex poup. Some differences were not statistically significant in all poups, but the direction of the differences was consistent. Males generally had less subcutaneous adiposity, but had a peatcr tendency to store proportionally more subcutaneous fat on the trunk than females. There were few differences for stature and skeletal dimensions. FN subjects were generally more endomorphic than EA subjects. Results were significant for all poups except the 5-19 year old females, in whom differences in somatotype were small but in the same direction as the other poups (peatcr endomorphy). . The estimated prevalence of obesity in FN was generally higher than in BA. However, siprificant differences in prevalences of obesity were evident only in the 20- 75 year old females. Among males and females 5-19 years, the prevalence of obesity (285th percentile age-specific NHANES II BMI) was 38.1% and 29.4% in FN males and females, respectively, and 21.3% and 16.9% in EA males and females, respectively. In FN adults 20-75 years, the prevalence of obesity (285th percentile NHANES II BMI for 20-29 year old people) was 51.4% in FN males, 58.8% in FN females, 39.0% in EA males, and 35.0% in EA females. There were differences between adult subjects classified as obese by the BMI only and the BMI+t1iceps skinfold in combination; however, the numbers were inadequate in the 5-19 year old poups to make comparisons. Also, there were too few subjects classified as obese by the triceps only to compare to the other poups. In 109 males, BMI obese subjects had greater TERs than the BMI-{triceps obese (p50.05). In general, endomorphy was also greater in the BMI+t1iceps obese group, and in the EA sample, mesomorphy was greater and ectomorphy was lower in the BMI+t1iceps obese sample. In EA males, the BMI-{triceps obese group also had greater bicondylar and biepicondylar breadths. Analyses of secular changes indicated significant decreases in stature with age inEAmales andfemalesbutnotinFN. Theestimateddecreasesinstaturewithagein FN were similar in magnitude to EA, but due to small numbers, the estimates were not significant. The statural loss due to aging (shrinkage) was estimated at 0.12 cm/year and 0.06 cm/year in EA males and females, respectively. Taking into account the estimated statural loss due to aging, positive secular trends of 1.0 cmldecade (p50.05) and 0.4 cmldecade (ns) in EA males and females, respectively, were estimated. A comparison of studies from 1953 to 1981 indicated that a secular trend in stature had occurred in Canada, and that the temporal trend in body mass appeared to mirror that of stature; however, there was no secular trend in the BMI. Mean BMIs of adults in the present study were greater than any reported study in Canada since 1953. There are two possible explanations for this finding: (1) the population of Temagami demonstrates significantly higher values for the BMI than the general population of Canada, and/or (2) there has been a significant increase in the BMI of Canadians over the past 15 years (1981 to 1996), and the population of Temagami is representative of the rest of Canada. It is possible that both scenarios may help explain the high BMls in the present sample. The results indicated significant familial resemblance in body size, physique, adiposity, relative fat distribution, grip strength and flexibility. Spousal correlations showed a lack of assortative mating (positive or negative) in this population. Further, the role of a shared living environment has apparently had minimal effects on spousal similarities in this population. 110 Interclass correlations between parents and offspring were significant and demonstrated familial resemblance for all variables, suggesting that genetic factors were operating on the familial associations. Additionally, intraclass correlations indicated significant sibship effects. Sibling conelations were typically higher or of the same magnitude as parent-offspring correlations, which suggested that the shared living environment may be important in explaining some of the variation within families. Results of partial correlation analyses indicated that mesomorphy was positively associated with right and left grip strength, whereas endomorphy was negatively associated with flexibility. There were few consistent correlations between body size and motor performance in children. The sum of skinfolds was negatively associated with the standing long jump in both sexes, and all measures of body size were negatively related to the flexed arm hang in boys. There was significant familial resemblance in grip strength and flexibility, and the inclusion of body size as a covariate in the correlation and regression analyses did not appreciably affect me results. 11] Conclusions The conclusions are best framed within the explicit hypotheses presented in Chapter 1. Hypothesis 1 There are significant differences between Canadians of First Nation (FN) and European ancestry (BA) in body size, physique, and indicators of health-related fitness. la) FN Canadians are heavier and demonstrate greater subcutaneous fatness than EA Canadians throughout childhood into adulthood. This hypothesis was partially supported. FN subjects were not significantly heavier in terms of body mass and the BMI, except in females M75 years. FN males 5-19 years were significantly fatter than EA males, and FN females 20-75 years were significantly fatter than EA females, in terms of subcutaneous fatness. There were few statistically significant differences among males 20-75 years and females 5-19 years; however, FN subjects were consistently fatter in all indicators of subcutaneous fatness. lb) There are significant differences in relative fat distribution and physique between FN and EA Canadians. This hypothesis was supported. In all age and sex groups, FN subjects had significantly greater TERs, and in all groups except females 5-19 years, FN subjects had greater WHRs. These results indicated that FN subjects had a more cenu'al subcutaneous fat distribution than EA subjects. Additionally, FN subjects were significantly more endomorphic than EA subjects in all groups except females 5—19 years. Among females 5-19 years, FN females were also more endomorphic, but the difference was not statistically significant. 1c) There are no differences in stature and other skeletal dimensions between FN and EA Canadians. This hypothesis was partially supported. Significant differences in stature and skeletal dimensions appeared only sporadically among the comparisons, with the 112 exception of females 20-75 years. Among adult females, all skeletal breadths were significantly greater in the FN group. FN adult females had a larger overall frame size than EA females. Hypothesis 2 Secular trends in body size are evident in FN and EA Canadians. 2a) There are significant secular increases in stature, mass and the BMI in FN and EA Canadians. This hypothesis was partially supported. There was a significant secular trend towards increasing stature in EA males; however, the secular trend in EA females and FN males and females was not significant. Comparisons among selected studies from 1953 to 1981 indicated that body mass had increased over time in a similar manner as stature. However, the BMI had not increased significantly over time in the Canadian population, with the exception of the present study, indicating that body mass has not increased more than would be expected given the secular trend in stature. The EMS in the present study were greater than earlier surveys in Canada, which could mean that a recent secular trend in the BMI has occurred in Canada since the last national survey (1981), or that this sample is not representative of the general Canadian population. Hypothesis 3 There is significant familial resemblance in body size, physique and indicators of health-related fitness in FN and EA Canadians. 3a) There is significant familial resemblance in body size, physique and indicators of health-related fitness in FN and EA Canadians. This hypothesis was supported. Spousal correlations indicated an absence of assortative mating (positive and negative) in this population. Correlations among nuclear family members and regression of the offspring on mid-parent values indicated significant familial resemblance in body size, physique, adiposity, relative fat distribution, grip strength, and trunk flexibility. Ethnic differences in familial 113 resemblance could not be determined due to insufficient sample sizes in the First Nation group. 3b) Estimated heritabilities for strength and flexibility are greater after body morphology is factored into the analyses as a covariate. . This hypothesis was not supported. The incorporation of stature, body mass, and the BMI into familial aggregation analyses for grip strength and trunk flexibility did not increase the correlation or regression coefficients. The incorporation of body size had little to no effect on the magnitude of the associations. 114 Recommendations for Future Research This study has demonstrated significant differences between Canadians of First Nation and European ancestry in components of health-related fitness; in particular, physique, fatness and relative fat distribution. Considerable evidence has been accumulated to suggest that excess fatness and a centripetal fat distribution are both independent risk factors for coronary heart disease and metabolic disorders. Similarly, there is also research which suggests that physique itself is related to risk factors for disease, or may be in and of itself, a risk factor. More research is required to better characterize the relationships between physique, fat distribution, metabolic fitness, and disease among First Nation Canadians, who are at increased risk for metabolic disorders. This study has demonstrated that differences between Canadians of European and First Nation ancestry are apparent in childhood and adolescence. Since many metabolic disorders such as obesity and diabetes may have their roots in childhood, emphasis should be placed on studying the growth characteristics of Native North Americans. The present study presents cross-sectional data on the growth of children; however, a longitudinal study may be more appropriate such that growth rates and other growth parameters may be estimated. More study is needed to better characterize activity patterns and daily energy intakes and expenditures in Native North Americans. These data are difficult to obtain, but their value becomes increasingly great. Clinical interventions are necessary to determine the effects of diet and activity programs among Native groups. Particular attention should be given to the genetic aspects of fatness and relative fat distribution, especially among Native North Americans. As the human genome becomes better characterized, ethnic variation at specific loci may help explain the greater susceptibility of Native North Americans to several metabolic diseases. 115 The best way to further the understanding of the etiologies of metabolic disorders among Native North Americans is through the use of family data. The ideal design would be a large scale longitudinal family study similar to the Quebec Family Study, which would include measures of dietary intake, physical activity, indicators of metabolic fitness, and anthropometry among members of extended families of Native North Americans. TABLES gags. EN 42 ...8 o... can. 3N .... jam .8. .0220. 9mm «.3 3.8 8.8 8. 838.2 832m .8. .85 ...o 98. SN 2. 88.2 2.82 :2 ...e .o .83... new a. : 98 e... ....8. SN mm 325.8 5.5m 5.8.30 E: ...e .n .83.... «.8. an. «.8 a... «.8. SN me 295.8 5...... 9:82 k2 £885 a 8.. 98 s..: ...... e... 2.... 2a 9. 99.5.8 .25 E: 2895 a 8.. new ...... ...... .... 0...... 2A 3 5.3. E: .89.... ... 8.. mew «d. «.6 ..m «.8. ...a .... es..; c.3895. .3230". 82.833 ... 8.52. new a... «...... N... no... ...«N 9. 9.82 85:3 18. 6220. ...8 5.. «...... 8-8 on 898.2 screen 2.... 8...... new 8.2. «.2. Raw 8 9.8.... 2.8.... .8. .88.. new 8.8 ..u 98. SN 5 83... 289.. {.2 ...e .e .895 .....N ...... ...: am 92.. SN 8 39530 5...... 5.8.20 .5. ...n .n .89.... IN. ...... 0.2. o... .3... cum on 99:28 5...... 9:82 E: £89.... ... 8.. ......N ...: ...... on am: ...a 2 29:28 5...... 2.2 189:... a. 8.. ...»... ...: «.8 a... n. 2.. 2a 2 5.3 E: 3895 a 8.. «em a. 2 ...8 m... ...8. 2a 3 es..; 5.3.2.... no...- flaauac am am 3 : 35.3 «Bauer—Sail“; 6.3.838“ 6.9.... ..oao :. one... u..- 228. .e. ...-o... Ea... 082...... e.- ..an 28.85... ....e: 3.3. ......- .e 83...... 8.8.8 9.2... in 85...... we. .8... .28... 3 53.858 ..u 352 116 117 TABLE 2.2 Components of health-related fitness. Morphological BMI Body Composition Subcutaneous Fat Distribution Flexibility Muscular Power Strength Endurance Motor Agility Balance Coordination Cardiorespiratory Exercise Capacity Heart and Lung Functions Blood Pressure Metabolic Glucose Tolerance Lipid Metabolism W Adapted from Bouchard & Shephard (1994) 118 TABLE 2.3 Evidence for familial resemblance in stature. 0‘0 533 0.12 0.26 to 0.54 0.01 to 0.2 American Utah Intraclass 529 0.37 0.14 to 0.43 0.87 to 0.48 CFS Interclass 1 8073 0.43 0.34 0.20 FOS Interclass 7948 0.39 0.44 to 0.51 0.47 to 0.54 Montreal interclass 997 0.25 0.34 to 0.43 intraclass 0.37 London Interclass 1083 0.46 to 0.67 Rural Interclass 1447 0.24 to 0.29 Colombia Belgium interclass 532 0.59 0.51 Montreal interclass 41 5 0.34 U.S. White Interclass 583 0.34 0.31 to 0.48 0.28 to 0.44 646 0.06 0.50 to 0.67 0.15 to 0.34 U.S. Black interclass FOS: Framingham Offspring Study; CFS: Canada Fitness Survey Rotimi 8r Cooper,1995 Ramirez,1993 Pérusse et al.,1988 Heller et al.,1984 Annest et al.,1983 Hawk & Brook,1979 Mueller 8 Tltcomb,1977 Susanne, 1975 Bouchard et al., 1980b Malina et al.,1976 Mueller & Malina,1976 Mueller 8 Malina,1980 Malina. et al.,1976 Mueller 8 Malina,1976 W 119 TABLE 2.4 Evidence for familial resemblance in body mass. Typeof Canalatirms fill‘ 3 0 " African Intraclass 533 0.15 0.30 to 0.35 0.36 to 0.52 Rotimi & Cooper,1995 CFS Interclass 18073 0.16 0.34 0.16 ' Pérusse et al.,1988 Montreal Interclass 997 0.18 0.22 to 0.35 Annest et al.,1983 intraclass 0.16 London Interclass 1083 0.30 to 0.47 Hawk 8 Brook,1979 Rural interclass 1447 0.28 to 0.37 Mueller 8 Titcomb,1977 Colombia Montreal Interclass 998 0.39 0.31 Biron et al.,1977 Belgium Interclass 532 0.54 0.34 Susanne, 1975 U.S. White Interclass 583 0.17 0.21 to 0.54 Mueller & Malina,1976 Mueller 8 Malina,1980 U.S. Black interclass 446 0.23 0.43 to 0.61 Mueller & Malina,1976 MW CFS: Canada Fitness Survey 120 . 3... d. 8... .8 82 8...... a. 5.8.2 8... e. we... :5. m3 8520.... .8... .m... 8... e. 8... .8 . 82 .232 a. 5.8.2 8.... e. 8... =5. 8... 892...... 9...; 8.: a... e. 8... 8... 2... es. .8. ...n .o .2: 2.... e. 8... on... 8... an; 8.. 8529:. 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C 3 8829.8 .8 on... 6:20:25! .520... 5 8659508. 35:... .3 §>m a.“ “405—. 122 TABLE 2.7 Evidence for familial resemblance In the BMI. . : : 2| : Rotimi & Cooper,1995 =I-0u on. African Intraclass 533 0.12 0.26 to 0.23 0.28 to 0.30 . o italy interclass 250 0.27 to 0.33 Antonella et al.,1994 Utah Intraclass 529 -0.05 0.21 to 0.46 0.03 to 0.29 Ramirez.1993 India Maximum 1691 0.37 0.28 to 0.55 0.24 Nirmala et al.,1993 Likelihood MPFS Interclass 1302 0.17 0.22 Moll et al.,1991 Intraclass 0.35 Norway interclass 74994 0.12 0.21 to 0.26 0.18to 0.21 Tambs etal.,1991 LRC Intetclass 3925 0.09 0.22 0.18 Price et al.,1990 CFS Interclass 18073 0.12 0.31 0.20 Pérusse et al.,1988 QFS Interclass 1698 0.10 0.26 0.23 Bouchard et al.,1988 Jerusalem Interclass 5740 0.08 0.33 0.22 Friedlander et al.,1987 Michigan Interclass 9226 0.12 0.27 Longini et al.,1984 Intraclass 0.23 to 0.38 FOS f Interclass 7948 0.19 0.09 to 0.27 0.21 to 0.27 Heller et al.,1983 Montreal Interclass 997 0.11 0.40 0.02 to 0.18 Annest et al.,1983 ___lmtaclaa CFS: Canada Fitness Survey; FOS: Framingham Offspring Study, QFS: Quebec Family Study; MPFS: Muscatine Poderosity Family Study; LFiC: Lipid Research Clinics program 123 myoEEm £§§+38$io£=8a=w+§=§a$ ”Em... ”floss. ...833.183.738.32.2oBa+..m...2..3+.a.=..83=2 Em» .2225... 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N2. 2.25 $2.... 2.2.8 ”w“... 225 2.22 2.5.5.8.... 825.. 8...... . . 2.59.8... 2.25. 8... .... ... 822 ...... 2 85 85 2.22.2“. .8 22532 w»... ......» 85...... ... 822 .85 ..85 2......on .... ..8 8252.... 8.... ...22 8... 8... ...... 2......on 58...... ... 828.. .....5 8... 8... 8222.5 5...... 822...... mu... 22...... 8... 3.... 85- 2......on 8.5.2220 ... .26.. 85- 8... ...... 2.0 28.2.... .8 8222... 2.........22 2.2.... ...... ... 8... 85 2 5.... 8... ...... ..3 N8. .....on 8... 2 8... 85 2 ...... m... ...... .....E 58. 858.... 2.2.... 2...... .8. ....2... .55 2 B5 8... ...... ..m. 8222... 2.8... 22.... ....5 ...... ...: «cm 2...; .w... 8... 2.0 ...... ..8 285 .w... t... ...... ......m «cm 2...; 5.: 8.22.8.2 ... ......22 8... 2... 2...... 8m 8.22... 22.... 5.: 2...... ”a... u n n u ... _ ..l—«O : ”a n a .— J u} .4. ”a tn u... q 1... u u: o« n. . =u1 . 58.322... ... 2.... 22:8 ... 2... 2.2.2.8.. 2...... ...... 59...... 2... ... 85.228: .222... .... 8:225 ....a 39: 126 TABLE 3.1 Age and «x dlstl'lbutlon of sublccts compand to nportod populations of Tommml and Bar Island. hmmml Number at - - Sum <1 5 200 65 55 15-29 170 55 43 30-44 230 68 81 45-64 300 65 68 265 130 16 22 149 133 29.2 29 15-29 52 12 1 5 27 51.9 30-44 37 6 1o 16 43.2 45-64 38 10 1o 20 52.6 255 19 2 3 5 25.3 mm 1:1 4 2 4 4 LL 4L4____ 'Data from Statistics Canada (1995) ”Data from Temagami First Nation band records 127 TABLE 3.2 Age and sex distribution oi the subsample participating in the analysis of measurement variability. Mean Age Age Range 11 FN EA (years) greats)— Males 34 5 29 22.2 4.7 - 75.3 Females 30 5 25 29.0 4.4 - 67.6 1913] 64 10 g 25.4 ’ 4.4 - 75.3 TABLE 3.3 Mean diiterences (X4), intraobserver technical errors of measurement (TEM) and intraclass correlation coefficients (rm...) between replicate measurements (n=64). Measure n X, TEM r... Mass 64 0.21 kg 0.72 kg 1.000 Stature 64 0.28 cm 0.54 cm 1.000 Sitting Height 63 0.63 0.95 0.997 Skinfolds 4 Triceps 63 0.19 mm 0.94 mm 0.994 Biceps 64 0.08 0.96 0.981 Subscapular 63 0.26 1.03 0.994 Suprailiac 63 0.07 1.13 0.988 Supraspinale 63 0.20 1.34 0.986 Medial Call 60 0.09 1.11 0.984 Abdominal 63 0.14 1.64 0.990 Email]: Bicondylar 63 0.02 cm 0.18 cm 0.989 Biepicondylar 64 0.03 0.10 0.995 Biacromial 64 0.12 0.57 0.995 Bicristal 64 0.09 0.58 0.993 W Flexed Arm 64 0.11 cm 0.40 cm 0.998 Relaxed Arm 64 0.13 0.51 0.996 Maximal Cali 60 0.01 0.34 0.998 Waist 64 0.29 1.15 0.997 Hip 64 0.22 1.43 0.996 W Endomorphy 63 0.06 0.20 0.995 Mesomorphy 60 0.03 0.17 0.993 W 64 0.04 0.13 0.295 128 .35.. 88:. .58.. 9...... .68.. .32.... 5...... 559.52 2.. 8.5.2. 5.8.. .12 .3589 H6...... .... .5 35.2. .53.. ..a ... c286,... .68.. .... ... 8.52... E. «N. ...... an a... 5.. 5... 5a; mN... ...... .N... o... 5... mo... .5... 50.5592 ..N... N .... B... 5... mm... 8... .5... :5. 699.3. ...N .... .... so ...... E... 8.8.“. $835.35 5N... ...... ...... ...... o... 3... .9305 ...... ...... 9... ..m... 8... B... .8585 N .... 2.... 8... N... N .... ..N... ...... 5.5.8285 5.... N..... N.... m... .2. so ...... 5.2.855 Hausa .4... 8... x... 5552...... ...... 3.... .N... N.N ...... N.N .... .8 5.8.2 9.... 8... 3... 5.8.8653 8... .5... ...... m.N 5.. .N... N... 3.935 3... ...N... Na... 55... ....N .5. NN.N 8.. 5.5.5825 .5... ...... 8... 885 N..... ..N... mm... N5... N.N .5... ...... EE 3... 88.... «33...... NN... ...o .5... 3... B... .5 3... £95: 9.55 NN... .N... ...... 9.... o... 3... ..N. .5 E... 2335 .N... «N... .N... .N... N... .... .... N... gs. ...... _ 52...: ......wm ... . . .N...—...... 41.4 41- ’30 g 882...... 8...... 588 5.5.39.5 :85... 2... 62...... weave—ea ... corona. one... 5.3 $5.... 52:23: .o 22.8 30.5.93 ..eEoeooahc. .o coatsano v.» ”55—. 129 TABLE 3.5 Sample sizes, age ranges, and Intraclass correlations (rm...) for replicate motor performance tests. Age Range Test n (years) r... Right grip 617 4.3 - 76.5 _ 0.997 Left grip 618 4.3 - 76.5 ‘ 0.998 Trunk flexibility 592 4.3 - 76.5 0.995 Standing long jump 102 5.9 - 14.8 0.991 W 29 5.1. 14.8 O-QQD—___. TABLE 3.6 Comparison of reliability coefiicients for replicate performance tests. M : :- .5 :‘: ”.3 ‘_ : 5;: Right grip 0.997 0.79 - 0.98 0.85 - .97 0.63 - 0.97 0.51 - .94 Left grip 0.998 0.80 - 0.97 0.79 - 0.93 Trunk flexibility 0.995 ‘ Standing long jump 0.991 0.72 - 0.95 0.50 - 0.93 0.88 - 0.84 0.82 - 0.94 ‘Malina and Mueller (1981) I'Maiina and Buschang (1985) TABLE 3.7 Least squares regression equations for the prediction of individual skinfolds in a sample of Canadians. Males __.Eennlaa : | : e“: 5‘ .3 ; ‘ : _:‘ 5: ‘z ‘ A : ‘2‘ .3 ‘: Constant - .311 5.937 2.291 7.744 4.929 Age 0.059 0.073 -0.042 0.098 -0.020 Subscapular 0.383 0.034 0.216 -0.070 Abdominal 0.178 0.007 -0.020 Suprailiac 0.265 -0.021 0.246 0.494 0.127 Supraspinale 0.241 0.612 0.124 0.108 0.029 - Medial Calf 0.082 0.036 -0.022 Triceps -0.120 0.399 0.406 0.092 0.548 Biceps 1.128 -0.264 0.354 0.043 0.169 R’ 0.77 0.66 0.63 0.76 0.61 u- 4-2L___L2§___2J_Q: 4.9.9 4-7L 130 TABLE 3.8 Skewness statistics for variables with skewed distributions and skewness statistics after log", transformation of the variables. Transformed W 1! S.E. Z n 1 S.E. Z a 5-19 yrs Mam Mass 0.79 0.25 3.20 <.005 ‘ 0.19 ' 0.25 0.76 ns BMI 0.82 0.25 3.33 <.005 ' 0.54 0.25 2.18 <.05 ' EXTREMITY 1.16 0.25 4.66 <.001 " 0.38 0.25 1.52 ns TRUNK 1.32 0.25 5.31 <.001 " 0.42 0.25 1.70 ns SUM ' 1.24 0.25 4.99 <.001 ‘ 0.47 0.25 1.87 ns TER 0.84 0.25 3.39 <.005 ' 0.32 0.25 1.27 ns Endomorphy 1.27 0.25 5.06 <.001 ‘ 0.40 0.25 1.58 ns Mesomorphy 0.60 0.25 2.40 <.025 ‘ -0.12 0.25 -0.46 ns Right Grip 1.01 0.25 4.11 <.001 ' 0.18 0.25 0.72 ns Left Grip 1.06 0.25 4.34 <.001 ' 0.12 0.25 0.49 ns Hang 1.46 0.37 4.42 <.001 ' -0.05 0.33 1.36 ns Eflmfllfifi Mass 1.47 0.27 5.43 <.001 ' -0.02 0.27 -0.08 ns BMI 1.52 0.27 5.50 <.001 ' 0.98 0.27 3.62 <.001 ‘ EXTREMITY 1.39 0.28 5.02 <.001 f 0.61 0.28 2.19 <.05 ' TRUNK 1.43 0.28, 5.11 <.001 ' 0.34 0.28 1.21 ns SUM 1.36 0.28 4.88 <.001 ' 0.53 0.28 1.91 ns TER 0.55 0.28 1.98 <.05 ' -0.19 0.28 -0.69 ns Endomorphy 1.09 0.28 3.92 <.001 ‘ 0.40 0.28 1.45 ns Mesomorphy 1.08 0.28 3.91 <.001 " -0.25 0.28 -0.88 ns Right Grip 0.58 0.27 2.11 <.05 " -0.41 0.27 -1.49 ns Left Grip 0.82 0.27 3.00 <.005 " -0.35 0.27 -1.27 ns Hang 1.29 0.37 3.49 <.001 ' 0.12 0.37 0.32 ns 20-75 yrs 9 Males Mass 0.70 0.17 4.18 <..001 " 0.17 0.17 1.02 ns BMI 0.65 0.17 3.89 <.001 ' 0.14 0.17 0.81 ns EXTREMITY 0.99 0.17 5.90 <.001 ' 0.00 0.17 -0.02 ns TER 0.60 0.17 3.64 <.001 " -0.01 0.17 -0.04 ns Ectomorphy 1.11 0.17 6.63 <.001 " -0.46 0.17 2.75 <.01 ‘ Bicondylar 0.44 0.17 2.63 <.01 ' 0.23 0.17 1.40 ns Biepicondylar 0.46 0.17 2.78 <.01 " 0.22 0.17 1.33 ns Eamales Mass 0.85 0.16 5.33 <.001 ' 0.35 0.16 2.16 <.05 ' BMI 0.81 0.16 5.00 <.001 ' 0.39 0.16 2.43 <.025 ' EXTREMITY 0.44 0.16 2.72 <.01 ‘ -0.30 0.16 -1.83 ns TER 2.23 0.17 13.40 <.001 ' 0.14 0.17 0.82 ns Ectomorphy 0.89 0.17 5.24 <.001 " -0.22 0.17 -1.32 ns Bicondylar 1.07 0.16 6.69 <.001 ' 0.77 0.16 4.78 <.001 ' y skewness statistic Z: standardized skewness statistic (skewness statistic / S.E.) 131 TABLE 3.9 Effects of age, by gender, on skeletal dimensions, circumferences, and AMA. Males Eamam B” x 100' R” x 109' 5-19 yrs Stature 93.4 ‘ 90.5 ' Sitting Height 90.9 " 88.4 ‘ SSR 22.2 ‘ 14.4 " SIL 90.1 ‘ 86.5 ' Biacromial Breadth 87.9 ' 83.5 " Bicristal Breadth 90.1 ' 74.7 ' Bicondylar Breadth 81.2 ' 48.9 " Biepicondylar Breadth 81.1 ‘ 66.6 ‘ Flexed Arm Circumference 70.8 ' 52.5 " Relaxed Arm Circumference 67.0 * 48.3 “ Maximal Calf Circumference 78.3 ’ 61.7 " Waist Circumference 64.7 ' 38.5 ' Hip Circumference 79.7 ' 65.1 " AMA 82.7 ' 53.5 ' 20-75 yrs Stature 15.3 ' 6.4 " Sitting Height 20.9 * 11.0 ' SSR 3.0 7.2 " SIL 4.6 " 1.4 Biacromial Breadth 8.8 ' 0.3 Bicristal Breadth 8.2 ‘ 9.5 ' Bicondylar Breadth 0.9 2.6 Biepicondylar Breadth 3.9 " 11.7 ‘ Flexed Arm Circumference 5.5 * 3.0 Relaxed Arm Circumference 6.0 ' 2.5 Maximal Calf Circumference 4.0 * 2.4 Waist Circumference 16.1 " 4.6 " Hip Circumference 2.8 2.7 AMA 5.8 ‘ 1.0 ‘Regressions significant at p50.05 'Ysage+age’+age’ 132 TABLE 3.10 Effects of age, by gender, on fatness, relative fat distribution, physique, grip strength, and trunk flexibility. Males Eemales R3 xJQO' R2 x 199' 5-19 yrs Body Mass 85.2 ' 60.1 ‘ BMI 42.9 ' 32.6 ' SUM 11.0 " 18.3 " TRUNK 15.2 ' 20.4 ' EXTREMITY 5.3 12.6 ‘ TER 35.1 ‘ 25.3 " WHR 28.8 ' 40.6 ' Endomorphy” 71.9 ' 76.4 ‘ Mesomorphy” 82.1 ' 72.5 ' Ectomorphy” 90.1 ' 83.2 ' Right Grip 86.7 r 74.9 * Left Grip 87.3 ‘ 70.8 “ Flexibility 2.4 6.3 20-75 yrs Body Mass 3.7 " 2.5 BMI 10.5 ‘ 2.6 SUM 6.4 " 3.4 TRUNK 9.6 ' 2.9 EXTREMITY 1.9 2.7 TER 6.9 ' 2.8 WHR 33.7 ' 6.4 ' Endomorphy” 54.2 ‘ 71.4 ‘ Mesomorphy" 68.8 ' 65.7 " Ectomorphyb 76.6 ‘ 75.9 " Right Grip 25.6 * 19.7 1 Left Grip 22.6 ' 19.2 ' Elaxibilifv 24.4 ' 9.5 ’ 'Regressions significant at ps0.05 'Y-age+age’+age’ ”Adjusted for the effects of age( as in note above), and the other somatotype components 133 TABLE 3.11 Distribution of sibship size among 266 families. m 1 2 3 4 5 6 Tom N EA 114 50 23 2 - 1 190 297 FN 46 21 6 3 - - 76 118 mm 160 71 29 5 - 1 266 .415 134 TABLE 3.12 Effects of age, mass, stature and the BMI, by gender, on grip strength and flexibility. Males Eemales R2 x 100 R2 x 100 5-19 yrs Fiexibility‘ 2.4 6.3 Flexibility” 4.5 6.7 Flexibility‘ 6.2 6.6 Flexibility” 2.9 7.3 Right Grip‘ 86.7 ‘ 74.8 ‘ Right Grip” 90.9 ' 95.9 ' Right Grip“ 99.0 ' 93.0 . Right Grip” 88.8 ' 81.9 " Left Grip' 87.3 " 70.8 " Left Grip” 92.0 ' 83.5 ' Left Grip" 89.2 ‘ 80.8 ‘ Left Grip“ 90.1 “ 79.0 ' 20-75 yrs Flexibliity' 24.4 * 9.5 ' Flexibility” 25.9 * 13.1 * Flexibility‘ 25.0 * 9.6 * Flexibility“ 25.3 ' 13.3 ' Right Grip‘ 25.6 ' 19.7 " Right Grip” 43.6 * 33.9 ' Right Grip° 33.9 ' 32.9 * Right Grip" 37.6 ' 27.1 ' Left Grip' 22.6 " 19.2 ‘ Left Grip” 42.1 ' 32.5 ' Left Grip‘ 32.3 r 30.7 * 1.911.600" 35-4 ' 165 “ 'Regressions significant at p50.05 'Yeage+age"‘+age° Wzage+age2+age3+mass ‘Y-age+age’+age’+stature Waage+age2+age°+BMi 135 TABLE 4.1 Sample sizes, means and standard deviations for age and indicators of body size. Age Body Mass Stature Sitting Height SSR A96 Group _Ms)_ _.(K£i)._ _.icml_ .4901).— _£%L_ _ML n .3 : |t: : I I t: 3| I ”(:‘z I I ”-13.3 I I Males EA 5-9 37 7.0 1.6 25.3 7.5 121.5 11.6 64.8 5.1 53.4 2.1 10-14 22 11.8 1.4 42.2 12.2 150.6 13.0 78.4 6.7 52.1 1.3 15-19 16 16.8 1.3 73.7 9.3 179.2 5.6 92.8 3.5 51.8 1.5 20-29 34 24.6 2.9 80.3 12.1 180.9 6.6 94.8 3.8 52.4 1.2 30-39 39 34.7 3.0 85.4 15.9 176.5 5.7 92.6 3.0 52.5 1.4 40-49 45 44.5 2.8 83.0 16.3 174.4 7.2 91.8 3.9 52.7 1.6 50-59 34 54.3 2.7 89.3 17.4 173.8 7.5 90.6 3.7 52.2 1.7 60-69 15 63.7 2.4 81.5 12.5 172.6 6.5 89.1 3.0 51.7 1.6 70-75 12 72.0 2.0 79.2 15.3 169.2 4.8 87.5 2.3 51.7 1.3 EN 5-9 8 6.8 1.3 29.8 10.0 125.5 9.4 66.0 6.2 52.5 1.9 10-14 6 11.2 1.4 45310.9 150.0 12.9 78.0 5.5 52.1 2.6 15-19 8 16.9 2.2 67.9 18.4 175.5 9.0 91.1 5.8 51.9 1.3 20-29 11 24.8 3.1 87.8 17.0 178.0 5.5 93.6 3.1 52.6 2.2 30-39 10 34.0 2.4 80.6 4.9 174.2 6.3 92.4 3.8 53.1 2.3 40-49 6 45.8 2.9 97.1 17.3 178.0 5.0 92.4 5.5 51.9 2.4 50-59 4 51.5 1.3 84.4 7.2 173.1 4.9 91.9 2.6 53.1 0.9 60-69 4 64.5 3.5 90.2 12.3 173.2 2.9 90.8 2.2 52.4 0.7 70-75 - Females EA 5-9 24 6.9 1 5 23.5 5.9 120.1 13.3 63.8 6.4 53.2 1.7 10-14. 20 11.7 1.2 43.8 14.0 150.1 9.3 78.8 5.3 52.5 1.2 15-19 16 16.7 1.5 61.7 18.9 165.1 8.3 86.7 4.9 52.5 1.0 20-29 19 24.4 3.1 66.2 14.1 162.1 5.9 85.8 4.1 52.9 1.9 30-39 54 34.1 2.9 66.1 12.7 163.0 5.8 86.4 3.2 53.0 1.1 40-49 40 44.4 2.5 73.1 19.5 163.6 5.9 87.2 3.8 53.5 1.7 50-59 33 53.5 2.9 70.5 14.6 161.4 5.4 85.6 3.1 53.0 1.1 60-69 24 64.4 3.2 66.3 12.5 158.4 5.3 83.3 3.1 52.5 1.1 70-75 10 71.4 1.3 65.2 16.2 158.4 5.0 83.0 3.2 52.4 1.0 EN 5-9 4 7.2 1.9 28.0 5.2 123.5 10.7 69.1 3.7 54.2 2.0 10-14 9 11.6 1.4 45213.5 152.4 11.1 79.6 5.6 52.3 1.1 15-19 6 16.5 1.6 62.9 28.0 162.1 7.9 87.3 5.4 53.9 0.9 20-29 17 23.1 2.5 74.8 17.3 165.5 4.4 87.6 3.0 52.9 1.0 30-39 12 32.9 3.0 77.8 14.0 162.7 5.4 86.3 2.9 53.0 1.4 40-49 12 44.4 3.5 73.6 15.0 158.7 6.3 85.0 2.7 53.6 1.2 50-59 5 52.8 2.5 78.9 11.3 161.6 4.0 85.8 2.5 53.1 1.7 60-69 7 62.6 2.9 76.5 13.2 165.4 3.8 85.0 2.1 51.4 0.9 70-25 1 71.1 70.3 153.4 78.9 51.4 SSR: sitting height / stature ratio (sitting height/stature X 100) 136 TABLE 4.2 Sample sizes, means and standard deviations for Indicators of fatness and relative fat distribution. BMI SUM TER WHR A96 Group Mm?)— _mm)_ .iirmim). .m— 4169! n WHR. Males EA 5-9 37 16.9 2.5 49.9 31.5 0.91 0.19 0.99 0.06 10-14 22 19.3 3.4 59.7 36.9 1.01 0.36 0.94 0.05 15-19 16 22.9 2.2 71.5 22.9 1.56 0.37 0.95 0.05 20-29 34 24.6 3.9 75.0 31.4 2.01 0.50 0.97 0.04 30-39 39 27.3 4.3 96.6 43.1 2.29 0.60 0.90 0.05 40-49 45 27.2 4.9 90.7 39.2- 2.26 0.54 0.91 0.05 50-59 34 29.5 4.9 106.4 39.2 2.53 0.63 0.96 0.05 60-69 15 27.7 4.1 90.5 31.1 2.37 0.62 0.96 0.03 70-75 12 27.6 5.0 97.1 29.0 2.10 0.33 0.95 0.05 EN 59 9 19.5 4.1 73.9 40.4 1.29 0.37 0.94 0.04 10-14 6 19.9 2.6 95.5 25.4 1.59 0.33 0.99 0.03 15-19 7 22.1 4.6 73.3 51.1 1.43 0.39 0.96 0.02 20-29 11 27.6 4.7 103.9 35.4 2.09 0.43 0.99 0.04 30-39 10 26.6 2.1 97.7 22.5 2.61 0.94 0.91 0.03 40-49 6 30.5 4.1 111.1 45.7 2.50 0.56 0.96 0.05 50-59 4 29.2 3.3 99.0 24.4 2.72 0.29 0.96 0.09 60-69 4 30.0 3.4 124.0 27.2 2.51 0.33 1.00 0.06 70-75 - Females EA 5-9 24 16.1 1.9 53.4 21.5 0.91 0.19 0.97 0.05 10-14 20 19.1 4.2 77.4 42.7 1.02 0.30 ' 0.79 0.07 15-19 16 22.3 4.5 97.4 43.2 1.19 0.29 0.76 0.06 20-29 19 25.2 5.2 117.5 51.5 1.12 0.27 0.75 0.04 30-39 53 24.9 4.9 109.7 43.0 1.19 0.29 0.77 0.04 40-49 40 27.3 6.9 136.6 49.9 1.24 0.24 0.90 0.06 50-59 33 27.0 5.1 135.4 43.9 1.26 0.30 0.90 0.07 60-69 24 26.4 4.6 137.4 44.2 1.29 0.45 0.92 0.06 70-75 10 25.9 5.3 126.6 53.2 144 0.99 0.92 0.05 EN 59 4 19.2 0.3 73.5 19.2 1.22 0.10 0.92 0.12 10-14 9 19.2 4.4 92.9 41.1 1.22 0.29 0.91 0.07 15-19 5 20.4 2.4 79.4 16.3 1.20 0.29 0.76 0.04 20-29 17 27.2 5.9 139.4 57.3 1.57 0.46 0.93 0.06 30-39 12 29.4 4.9 197.5 40.7 134 0.23 0.95 0.06 40-49 12 29.3 6.1 142.0 32.9 1.57 0.39 0.94 0.05 50-59 5 30.2 3.9 170.0 32.0 1.27 0.06 0.97 0.04 60-69 7 29.1 5.5 156.7 57.0 1.43 0.23 0.99 0.03 70.75 1 29.9 4490 2.10 0.91 SUM: sum of six skinfolds, (triceps+biceps+mediai calf+sumcapular+suprailiac+abdominai); TER: trunk I extremity ratio (subscapular+suprailiac+abdominai /triceps+biceps+medial calf); WHR: waist I hip circumference ratio 137 TABLE 4.3 Sample sizes, means, medians, and standard deviations for extremity skinfolds. Triceps Biceps MediaICalf EXTREMITY A96 Group _iumi_ _imm)___ ____imm)__ _.(mmi_ Males EA 5-9 37 10.7 90 5.4 4.6 4.3 2.3 9.3 8.0 5.0 23.9 21.2 11.6 10-14 22 11.7 9.0 5.4 5.3 4.0 3.3 11.1 9.3 5.2 28.1 22.2 13.5 15-19 16 12.5 10.7 5.3 5.3 5.0 2.1 10.4 8.9 3.8 28.3 25.5 9. 9 20-29 34 11.3 11.7 4.4 4.4 3.7 2.5 9.3 8.1 4.6 25.0 23.8 10. 3 30-39 38 129124 5.5 6.1 5.2 3.7 10.410.0 5.2 28.7 28.0 12.2 40-49 45 12.8 12.2 5.5 5.2 4.5 2.3 9. 9 8.6 5.2 27.0 25.6 10.6 50-59 34 13.4 11.0 6.3 7.3 5.6 4.1 10.6 9.0 5.8 30.4 25.0 14.0 60-69 14 12.2 10.6 5.2 6.0 5.3 2.6 8.9 8.2 4.2 27.0 22.6 11.4 70-75 12 13.1 12.8 5.2 6.5 6.0 2.7 9.0 9.1 3.5 28.6 30.8 10.5 EU 5-9 8 13.3 10.1 6.5 6.5 5.9 2.2 11.0 10.8 3.3 30.8 26.5 11.4 10-14 6 15.717.6 3.6 7.5 7.6 1.1 13514.5 3.7 36.6 38.5 7.4 15-19 7 12.0 9.0 7.5 5.2 4.6 2.6 11.6 10.0 5.8 28.8 24.2 15.4 20-29 11 14.814.0 4.8 6.8 6.0 2.9 11.911.0 4.4 33.5 30.8 10.6 30-39 10 11.4 11.5 4.0 4.7 4.1 1.6 9.0 8.3 4.0 25.1 24.7 8. 5 4049 6 14.9125 5.9 7.1 7.1 2.9 9.5 8.0 4.5 31.5 28.0 12.1 50-59 4 12.5 7.2 3.6 6.5 4.6 2.1 7. 7 6.0 2.9 26.6 18.0 7.6 60-69 4 15.4 15. 8 5.2 8.7 7.6 2.6 12. 2 10.2 5.4 36.2 33.8 11.6 70-75 - - Females EA 5-9 23 12.6 11.2 4.0 5.4 5.0 1.9 11.0 9.6 4.2 29.2 26.3 9.6 10-14 20 14.711.7 6.2 7.1 5.2 4.5 157127 7.7 37.5 28.2 17.7 15-19 16 19.1 15.8 8.0 7.9 6.5 3.7 17.417.2 8.3 44.4 39.6 18.7 20-29 19 24.1 21.4 9.2 9.2 6.0 6.6 21.719.0 8.3 55.1 43.4 22.7 30-39 53 23.1 22.0 7.5 9.9 7.8 5.9 18.8 17.6 7.3 51.7 46.2 19.0 40-49 40 27.0 24.4 8.6 13. 3 10.0 7.5 21.7 19.2 7.7 61.4 55.0 22. 4 50-59 33 26.2 25.8 7.6 12. 5 12. 0 6.0 22.3 21.2 7.3 60.9 62.0 18. 8 60-69 23 26.2 23.0 8.3 13.0 11.2 6.7 21.5 21.4 7.5 60.7 55.4 20.7 70-75 10 23.3 20.0 11.6 12.3 10.3 7.6 19.2 17.8 8.0 54.7 49.4 25.5 EN 5-9 3 15.617.0 4.7 6.3 6.2 1.6 11.011.4 1.4 32.9 34.6 7.6 10-14 9 14.6 15.2 4.5 7.8 6.0 4.0 13.6 14.4 5.2 36.0 35.2 13.3 15-19 5 14.5 14.8 3.6 5.0 5.2 1.4 17.2 16.4 6.1 36.7 36.6 10.6 20-29 16 25.7 24.9 10.3 12.8 11.5 7.8 19.7 16.8 11.3 58.4 56.6 28.9 30-39 11 31.4 30.4 7.4 16.6 17.4 4.7 23.8 22.0 6.8 71.8 77.4 16.5 40-49 12 25.2 25.0 7.2 13.9 10.6 9.3 18.6 16.1 6.8 56.5 56.2 16.1 50-59 5 30.1 32.0 6.5 19.1 16.4 8.4 26.0 26.4 1.9 75.2 76.2 15.2 60-69 7 30.1 32.0 11.8 14.4 14.6 8.0 21.5 21.6 9.4 66.0 70.2 26.8 70-75 1 20.4 7.2 20.2 47.8 EXT REMITY: sum of extremity skinfolds (biceps+triceps+medial calf) 138 TABLE 4.4 Sample sizes, means, medians, and standard deviations for trunk skinfolds. Subscapular Suprailiac Abdominal TRUNK A96 Group 4mm; __..(um)___ _tmmi_ __.(um)___ Males EA 5-9 37 7.4 5.8 4.8 6.0 40 5.2 10.9 8.8 8.5 24.4 19.2 18.3 10-14 22 8.5 6.1 6.2 9.3 5.9 7.9 12.8 7.9 10.8 30.6 19.7 24.1 15-19 16 10.7113 2.7 10.811.4 3.4 21.8 22.4 9.0 43.2 47.2 14.2 20-29 34 14.1 12.0 6.8 12.2 11.1 6.7 23.6 24.5 10.6 50.0 49.8 22.3 30-39 39 19.8 18.0 10.0 13.7 12.4 6.7 32.1 33.4 12.8 64.1 61.6 25.9 40-49 45 18.7 17.4 10.2 13.7 12.4 6.9 29.9 32.0 11.0 62. 3 63.2 25.4 50-59 34 25.2 24.5 9.9 15.7 15.1 6.9 33.7 34.0 10.3 74.6 73.4 25.0 60-69 15 21.0 18.8 11.0 14.1 10.0 9.6 34.7 33.0 12.7 69.7 58.2 31.4 70-75 12 17817.2 6.3 11.210.7 4.6 29.4 30.6 9.2 58.5 57.9 18.8 E]! 5-9 8 12.8 8.2 9.5 10.4 7.0 7.6 19.9 16.6 12.3 43.1 31.6 29.2 10-14 6 18.7 20.3 6.9 14.3 13.8 6. 7 25.9 25.8 8.0 58. 8 62.4 19.3 15-19 7 12.6 11.5 6.8 11.9 7.2 10.1 20.0 12.6 14.6 44.5 29.6 36.0 20-29 11 20.2 21.4 10.1 17.0 19.6 7.9 33.2 36.4 9.8 70.5 78.4 26.1 30-39 10 19.4 19.7 5.8 11.7 11.9 3.5 31.5 31.9 9.5 62.6 67.9 17.1 40-49 6 28.4 27.4 14.1 15.0 13.8 7.1 36.1 37.6 13.9 79.5 78.8 34.6 50-59 4 19.6182 5.8 15314.1 3.2 36.5 34.3 8.8 71.4 66.6 17.0 60-69 4 28.5 24.7 9.9 22.1 22.2 5.4 37.3 36.5 2.9 87.8 86.4 15.6 70-75 - Females EA 5-9 22 7.5 6.4 3.3 6.6 5.4 3.8 10.2 7.9 5. 7 . 24.2 19.9 12.6 10-14 20 11.9 8.8 7.2 11.7 8.2 8.8 16.4 13.3 10.7 39.9 31.5 26.0 15-19 16 14.9 11.8 8. 3 14.1 10.8 8.5 23.3 19.4 10.1 52.5 46. 2 26.2 20-29 19 20.8 17.8 10.4 15.7 13.2 9.8 26.0 22.6 11.1 62.4 54.6 30.3 30-39 46 19.6 16.0 10.2 14.2 10.3 8.6 25.7 23.7 10.0 59.5 51.2 28.0 40.49 39 24.9 26.0 10.0 19.2 18.0 10.0 31.5 30.2 10.2 75.2 74.0 28.0 50-59 31 23.9 23.2 9.8 20.0 19.8 9.1 31.5 33.0 9.5 75.5 75.6 26.6 60-69 23 22.3 20.2 9.6 19.5 18.4 9.6 33.4 33.2 9.4 75.1 73.8 27.0 70-75 10 18.5 15.8 9.7 18.9 18.9 10.4 31.9 31.7 10.2 69.3 63.9 28.6 EN 5-9 3 10.8 11.8 3.8 12.3 13.0 5.1 17.5 18.2 3. 3 40.6 45.2 11.6 10-14 9 13.3 11.2 7.7 14.2 10.0 10.2 19.3 15.8 10.6 46.8 37.8 28.1 15-19 6 12.6 11.2 3.7 16.2 11.8 12.7 23.7 19.5 12. 2 42.7 42.0 9.0 20-29 16 27.7 31.6 11.4 22.8 26.2 10.9 32.9 32.1 8.0 82.2 86.8 30.0 30-39 10 32.4 34.1 9.4 27.1 27.3 11.2 36.2 37.5 7.4 95.7 98.8 25.7 40-49 12 29.0 28.3 10.3 22.9 22.1 8.8 35.6 36.0 6.7 89. 7 85.8 23. 5 50-59 5 29.5 29.4 5.5 27.2 26.6 6.6 38.0 39.6 6. 2 94. 8 95.6 16.9 60-69 7 27.7 25.6 12.7 24.9 27.8 9.3 38.2 40.0 10. 6 90. 7 87.6 31.3 20-75 1 22.0 31 .0 47.2 400. 2 TRUNK: sum of trunk skinfolds (subscapular+suprailiac+abdominal) 139 TABLE 4.5 Sample sizes, means and standard deviations for skeletal breadths. Bicondylar Biepicondylar Biacromial Bicristal HSR A96 Group _m1_ __(9m)__ __.iqni_ _icm)._ _fiizi_ Hi: :1 U ”9.: I I ”.251 | 11.2:1 | 11.2.:1 D Ilales EA . 5-9 37 7.5 0.8 5.1 0.5 27.9 3.0 20.2 2.3 72.6 3.7 10-14 22 8.9 0.7 6.1 0.6 33.8 3.4 24.6 3.1 72.8 4.2 15-19 1 6 9.7 6.9 7.1 0.5 41.5 1.9 30.0 1.8 72.3 3.5 20-29 34 9.7 0.7 7.2 3.5 44.0 2.1 30.6 1.8 69.6 3.3 30-39 39 9.9 0.7 7.3 0.4 44.0 2.5 31.8 2.7 72.2 4.1 40-49 45 9.7 0.7 7.2 0.5 43.1 2.4 31.8 2.6 73.5 3.6 50-59 34 9.9 0.7 7.5 0.6 42.7 2.6 33.1 2.9 77.4 5.3 60-69 15 9.9 0.8 7.4 0.6 42.4 2.4 31.9 2.9 75.8 5.4 70-75 1 2 9.8 0.7 7.4 0.3 41.3 1.9 32.6 2.1 79.0 3.7 EN 5-9 8 7.6 0.7 5.3 0.3 29.4 2.6 20.6 2.5 69.9 3.5 10-14 6 9.0 0.7 6.3 0.5 33.9 1.7 24.0 2.3 70.6 4.2 15-19 8 9.5 0.8 7.1 0.6 42.0 4.0 29.0 3.7 70.4 4.2 20-29 11 10.1 1.0 7.5 0.4 44.6 3.3 32.1 2.3 72.0 2.9 30-39 10 9.9 0.3 7.2 0.3 _ 43.2 1.7 30.9 2.2 71.4 4.5 40-49 6 10.1 0.8 7.6 0.4 45.1 1.3 34.2 2.2 76.0 5.5 50-59 4 10.0 0.5 7.5 0.4 43.3 2.3 33.0 1.5 76.1 1.6 60-69 4 9.9 0.7 7.4 0.4 42.1 2.4 34.3 1.4 81.4 2.2 70-75 - Females EA 5-9 23 7.1 0.7 4.8 0.5 27.6 2.5 20.0 1 9 72.6 3.2 10-14 20 8.4 0.8 5.8 0.3 33.9 3.0 25.2 3.1 74.3 4.0 15-19 16 8.7 1.0 6.1 0.6 37.4 2.0 28.9 3.1 77.2 5.5 20-29 1 9 9.0 0.7 6.1 0.4 37.7 1.6 29.1 2 1 77.0 4.6 30-39 54 9.1 0.9 6.2 0.4 38.2 2.0 30.4 2.6 79.6 4.8 40-49 40 9.5 1.3 6.5 0.5 38.6 2.4 31.4 3.5 81.0 6.4 50-59 33 9.5 0.9 6.4 0.4 38.0 2.1 32.1 2.8 84.5 5.9 60.69 24 9.4 0.7 6.6 0.5 37.9 1.9 31.9 2.6 83.8 5.7 70-75 10 9.3 0.8 6.5 0.5 38.3 1.6 32.5 2.0 84.9 3.3 E]! 5-9 4 7.3 0.3 5.0 0.2 28.3 1.6 20.9 1.9 73.5 3.5 10-14 9 8.3 0.5 5.7 0.3 34.2 3.5 26.0 2.9 76.1 2.3 15-19 16 8.7 1.0 6.1 0.6 37.4 2.0 28.9 3.1 74.2 6.2 20-29 17 9.5 1.3 6.4 0.4 39.6 2.1 31.4 3.2 79.4 6.3 30-39 1 2 9.7 0.8 6.5 0.3 39.8 1.7 32.4 2.0 81.4 4.5 40-49 12 9.6 1.0 6.6 0.5 38.6 1.8 32.0 3.0 82.9 5.3 50-59 5 9.8 0.4 6.9 0.1 39.6 1.5 34.5 1.5 87.3 4.4 60-69 7 9.9 1.1 6.9 0.7 40.2 1.4 34.4 2.2 85.5 5.0 70-75 1 9.2 6.3 48.2 34.0 ' 89.0 HSR: hip I shoulder ratio (bicristal/biacromial x 100) 140 TABLE 4.6 Sample sizes, means and standard deviations for circumferences and AMA. Age Flexed Arm Relaxed Ann Maximal Cali Waist Hip AMA Group _.(9£D)_ _icmi_ .1901)— _L9mL_ m— __£90fl_. * l ' : : “‘1‘; I I ”5:. I 11.3.3 I I ”at. I 14.1 :| I Males EA 5-9 37 20.2 2.9 19.0 2.8 24.5 3.1 58. 4 6.9 65.9 8.0 19.67 3.8 10-14 22 24.3 3.7 22.7 3.6 30.0 3.4 67. 5 9.5 80.0 9.1 29.1 7.1 15-19 16 32.3 2.7 29.4 2.8 35.7 2.3 82. 9 6.8 97.1 5.4 52.0 9.4 20-29 34 34.6 3.3 31.8 3.5 36.7 3.1 86. 2 7. 7 99.3 5.6 64.1 14.2 30-39 39 36.6 3.5 33.0 3.5 38.4 3.4 92. 5 10. 9 102.0 7.4 66.4 12.8 40-49 45 35.5 3.2 32.3 3.2 37.0 3.7 92. 9 11.7 100.9 7.2 64.4 11.8 50-59 34 36.2 3.5 33.1 3.3 37.8 2.8 100.1 12. 5 104.3 9.0 67. 0 12. 0 60-69 15 35.3 5.3 31.3 3.2 37.1 4.1 97. 2 9. 5 101.6 7.3 60. 4 10. 3 70-75 12 33.6 3.8 30. 3 3.5 34.8 3.1 96. 3 10. 4 101.2 7.3 55. 0 10. 9 EN 5-9 8 22.1 4.2 20.3 3.5 25.5 3.2 64.7 10.6 68.7 9.2 20.9 4.6 10-14 6 26.2 2.7 24.3 2.6 30.0 3.3 73.0 7.7 83.0 8.1 ‘29. 9 5.4 15-19 8 32.7 7.2 29.4 6.6 34.7 3.6 80.7 11.4 93.2 11.5 44.7 7.5 20-29 11 36.7 4.2 32.7 3.6 37.8 3.1 92.3 11.5 104.0 8.4 63.4 13.0 30-39 10 35.5 2.4 32.3 2.5 36. 3 1.5 91.0 2. 7 99.8 3.2 66.1 11.6 40-49 6 37.8 3.3 34.7 3.0 37.7 4.3 102.1 11.8 106.1 8.9 72.4 16.8 50-59 4 35.1 3.2 33.0 3.6 36.4 1.0 95.7 3.9 100.1 3.4 68.217.6 60-6919 35.4 5.0 31.5 3.1 36.9 3.9 98.5 9.0 102.0 6.7 61.314.2 70-75 - Females EA 5-9 23 19.9 2.2 18.8 2.2 24.5 2.5 56.0 4. 8 64. 8 7.1 17.8 3.3 10-14 20 24.4 3.5 23.1 3.3 30.7 4.1 65.1 10. 5 83. 0. 10. 9 27.4 6. 0 15-19 16 28.9 4.9 27.3 5.2 34.4 3.9 72.4 10.7 94.8 11.0 37.0 12. 3 20-29 19 30.2 4.0 28.7 3.8 35.7 3.4 77.1 9.6 101.9 10.1 35.6 6.0 30-39 53 30.8 3.7 29.2 3.8 35.8 3.1 77.5 10. 9 100.6 10.9 38.7 8.1 40-49 40 32.2 4.4 30.9 4.8 35.9 3.9 84. 4 14. 7 105.5 13.9 40.7 10. 4 50-59 33 32.3 4.1 30.0 3.7 35.6 3.3 85. 2 13.5 106.1 11.8 38.1 7.1 60-69 24 32.1 4.6 30.2 4.6 35.0 2.6 84. 9 11.6 103.6 9. 7 40.1 8.7 70-75 10 31.8 6.3 29.5 4.9 34.3 3.1 83.5 11.0 101.3 10.6 39.4 7.1 EN 5-9 3 22.0 2.3 20.5 1.5 26.2 9.0 63.3 2. 4 78.6 14.6 19.5 0.8 10-14 9 24.1 3.0 22.9 3.4 29.8 3.1 67.7 12.4 83.4 11.2 27.1 7.2 15-19 6 28.7 5.6 26.9 5.7 34.1 6.7 72.3 14.1 94.9 16.2 32.1 3.9 20-29 17 32.1 4.3 30.3 4.2 36.7 4.9 87.6 11.8 105.9 12.8 40.8, 6.9 30-39 11 34.8 4.0 32.1 3.0 37. 5 3.2 92.4 10.7 108.8 8.8 39. 4 5. 8 40-49 12 34.4 5.4 32.0 5.1 35.5 3.3 89.6 12.2 106.9 13.3 42.2 10.0 50-59 5 33.1 2.2 30.5 2.1 36.2 1.9 95.5 10.3 109.2 9.8 35.2 2.5 60-69 7 34.5 5.6 32.3 5.3 34.9 2.6 97.4 11.0 110.3 10. 2 41. 8 6.3 70-75 1 28.7 27.8 33.0 415.5 115.5 36.4 AMAzestimatedarmmusciearea 141 TABLE 4.7 Sample sizes, means and standard deviations for Heath-Carter anthropometric somatotype components. Age Group Endcmnmhx MW! 5912032621]! .1151 n MeanJQ—_Mem SD .Maan__SD__ Males EA 5-9 34 3.2 1.6 4.9 1.0 2.1 1.2 10-14 22 3.0 1.7 4.3 1.3 3.5 1.6 15-19 16 3.3 1.1 4.3 1.2 2.8 1.0 20-29 34 3.7 1.5 4.9 1.6 2.4 1.4 30-39 39 4.7 1.9 6.2 1.4 1.3 1.0 40-49 44 4.5 1.8 5.9 1.6 1.3 1.2 50-59 34 5.3 1.8 6.6 1.4 0.8 0.8 60-69 15 4.9 2.1 6.6 2.3 1.0 1.3 70-75 12 4.5 1.5 6.0 1.6 1.1 1.1 EN 5-9 8 4.6 2.3 5.0 1.0 1.8 1.2 10-14 6 5.4 1.6 4.9 1.3 2.4 1.5 15-19 7 3.5 2.3 4.3 1.4 3.1 1.5 20-29 11 5.1 1.7 6.2 1.8 1.3 1.2 30-39 10 4.6 1.3 6.0. 1.1 1.2 0.9 40-49 6 5.6 1.8 6.6 1.3 0.5 0.4 50-59 4 4.9 1.2 6.3 1.5 0.8 1.1 60-69 4 6.3 1.4 6.2 1.7 0.4 0.6 70-75 - Females EA 5-9 22 3.6 1.2 4.3 1.1 2.5 1.2 10-14 9 4.5 1.9 3.3 0.9 3.3 1 .8 15-19 15 4.9 1.8 3.8 1.4 2.4 ' 1.5 20-29 19 5.9 2.0 4.6 1.7 1.5 1.3 30-39 44 5.6 1.8 4.5 1.6 1.7 1.2 40-49 34 6.4 1.7 5.0 1.9 1.5 1.2 50-59 31 6.6 1.8 5.4 1.6 1.0 1.1 60-69 23 6.5 1.7 6.0 1.5 0.8 0.8 70-75 10 5.9 1.9 5.7 1.8 1.0 0.6 EN 59 3 4.6 1.0 4.5 0.6 1.6 0.5 10-14 9 4.5 1.9 3.3 0.9 3.3 1.8 15-19 6 5.3 2.2 4.1 2.6 2.3 1.6 20-29 1 2 6.7 2.3 5.3 2.1 1.2 1.1 30-39 9 8.1 1.7 6.3 1.7 0.5 0.8 40-49 10 7.5 1.4 6.2 1.3 0.4 0.4 50-59 4 7.8 1.2 6.1 0.9 0.3 0.3 60-69 7 7.3 2.3 5.9 2.5 1.0 1.5 711-25 1 7.1 5.3 0.1 142 TABLE 4.8 Sample sizes, means and standard deviations for grip strength and trunk flexibility. A98 Grow mmamwsm— Miami)— Millenn— Jasi n Mean—SD n New 50 Mean.._SD_ Males EA 5-9 37 13.2 4.2 37 12.3 4.1 35 28.1 4.5 10-14 22 24.9 9.0 22 24.0 7.8 22 23.8 8.6 15-19 16 52.1 8.6 16 50.2 8.5 16 29.5 6.0 20-29 34 60.4 8.2 34 57.4 8.1 34 32.4 6.6 30-39 39 58.9 8.4 39 58.0 8.3 38 31.7 7.6 40-49 45 57.8 9.4 45 55.1 9.5 42 26.7 9.0 50-59 34 53.5 9.6 34 51.3 9.8 34 22.0 7.5 60-69 15 50.2 9.7 15 47.8 9.1 1 2 23.0 7. 0 70-75 12 39.8 6.9 12 39.2 5.2 11 16.8 101 EN 5-9 8 11.9 4.0 8 11.3 4.1 8 26.8 5.5 10-14 6 21.7 5. 2 6 20.8 4.3 6 24.3 7.2 15-19 8 49.0 11.7 8 45.3 14.0 8 28.3 6.6 20-29 11 59.0 10.7 1 1 56.4 10.6 11 29.4 11.2 30-39 9 52.1 5.9 10 49.5 10.6 10 27. 9 9. 5 40-49 6 49.6 8.9 6 47.9 10. 5 6 23. 1 12. 6 50-59 4 50.6 7.3 4 47.5 9. 9 4 19. 3 8. 8 60-69 4 41.8 7.8 3 31.3 11.9 4 15.1 10. 6 70-75 - Females EA 59 23 11.4 3.4 23 10.8 3.3 21 28.8 5.0 10-14 20 23.0 5.2 20 21.0 4.9 20 ‘ 28.8 6.7 15-19 15 33.4 7.8 15 30.2 8.4 14 30. 6 5.8 20-29 19 32.9 6.6 19 31.1 6.1 19 30. 9 7.8 30-39 54 34.8 5.9 54 32.9 6.0 52 32. 3 8.4 40-49 39 34.0 5.8 40 31.7 5.6 38 27.1 8.6 50-59 32 30.4 6.6 33 28.5 6.6 32 25.1 9.1 60-69 23 29.2 5.0 22 27.0 6.2 20 27.6 8.7 70-75 10 24.8 4.5 10 24.2 4.8 8 23.3 4.7 EN 59 4 10.8 2.3 4 10.8 2.2 3 30.3 4.5 10-14 9 20.7 6. 9 9 ' 19.0 4.7 9 27.9 6.4 15-19 6 33.1 10. 0 6 29.1 8.4 6 31.0 6.3 20-29 17 36.1 6.2 17 33. 2 5.4 17 30.3 8.7 30-39 12 36.0 7.0 12 34. 3 6.7 1 2 34.5 9.5 40-49 12 33.4 7.9 12 31.0 7.6 12 29.6 6.6 50-59 5 29.4 4.8 5 28.6 3.2 5 29.0 4.2 60-69 7 27.6 3.7 7 23.5 3.9 7 24.2 A 4.5 70-75 1 1 6.0 1 14-0 J 9.5 I43 mN a «MK Ne v.2. FN.. on DNA. #01. JV a and h? AWN Row 5* g and NOS 0. ad ad a mud on; 9.. a cm or ad 6.: mp Zn 556 meg. mm 0.6. N.N. mm 2.6 mm. w PM a mm #0 md ad vn (m ee.eEeu 06.. 04.5 mm 0.0. m.m_. av 5.0 cc... mm ..p mm N0 ed 06 N0 .30... 9.... ~95 N. ad .6 GP and #0.. 0.. N_. MN 0. ad m6 0.. 2.... NO; 3.5 ov 069 Yb. mm and we; av ..w mu m4 9N ad a? (w as?! amends: nail: $333.. .586 Edmund; 3334333313333: .eue .o flee» a...» c2220 ... sees: LouoE use one .0. ace—.35.. ...—eves: use eceeE .eefie 0358 Re m..n<._. 144 TABLE 4.10 Anthropometric z-scores“ and results of t-tests for differences between males and females, and between EA and FM samples. Group jA JN n Mean—SD n Mean__SD___ Males - 5:19.163 _ z—stature 75 0.49 1 .05 22 0.98 0.72 ‘ z-SSR 74 -0.66 1 .23 22 -0.73 1 .48 z-mass 75 0.68 1.20 21 1.44 1.62 ‘ z-BMI 75 0.40 1.02 21 0.82 1.33 z-triceps 75 0.45 1.06 21 0.80 1.10 z-subscapular 73 0.44 0.87 21 1.47 1.72 ‘ z-AMA 75 0.02 0.82 21 0.15 0.87 29:15.15 z-stature 167 0.47 0.92 35 0.42 0.71 z-SSR 167 -0.41 1.03 35 -0.21 1.19 z-maas 166 0.89 1.31 35 1.14 1.13 z-BMI 176 0.52 1.15 35 0.80 0.87 z-triceps 175 0.13 0.81 35 0.31 0.70 z-subscapuiar 177 0.37 1.10 35 0.75 1.04 z-AMA 175 0.12 1.06 35 0.26 0.70 Females 5:12.166 z-stature 60 0.65 1.32 19 0.75 1.03 z-SSR 60 -0.54 0.84 18 -0.10 0.96 z-mass 60 0.74 1.76 19 1.11 2.11 z-BMi 60 0.26 1.06 18 0.39 1.05 z-triceps 59 0.21 0.96 17 0.08 0.70 # z-subscapular 58 0.39 0.94 17 0.54 0.85 # z-AMA 59 0.11 1.14 17 0.03 0.90 2915.166 z-stature 170 0.41 0.81 53 0.55 0.86 z-SSR 167 -0.41 1.01 53 -0.38 0.87 z-mass 168 -0.43 1.30 ii 53 0.21 1.20 if ' z-BMI 178 0.37 0.98 54 0.99 1.05 ' z-triceps 178 0.05 0.87 51 0.47 1.00 ' z-subscapular 1 68 0.24 0.84 51 0.98 0.98 ' leMA 1 79 0.09 0.79 51 0.35 0.19 . 'FN and EA samples significantly different at pS0.05 «Male and female samples significantly different at different at p50.05 ‘Stature, mass, and SSR were z-standardized using Canadian reference data (Health and Welfare Canada, 1 980) ”BMI, triceps and subscapular were z-standardized using NHANES Ii reference data (Najjar and Rowland, 1987) ’AMA z-standardized using NHANES l and II reference data for Whites (Frisancho, 1990) 145 TABLE 4.11 Anthropometric z-scores‘ for FN adults 20-75 yrs standardized using FN reference data from Canada and results of t-tests for differences between males and females. _.Mala__ __Eamale__ n Mean—SD n MeanJL—_ z-stature 35 0.70 0.93 53 0.99 1.00 z-mass 35 1.38 1.19 53 0.69 1.09# 1:558 3.5__Q.0_1_LI16.___5§__-.Q.31_Q.6.0__ #Male and fernaie samples significantly different at different at pS0.05 'Stature, mass, and SSR were z-standardized using FN reference data (Health and Welfare Canada,1980) ‘ TABLE 4.12 Results of the secular trend regression analysis for stature. S.E. S.E. 6:01.19 Equatim tern) (anal—(SM I .0 55111591909 EA Males y = 184.6 - .209 age .03 .41 <.001 ' EA Females y = 166.3 - .098 age .03 .23 .002 " FN Males y a 179.6 - .097 age .07 .24 .17 FN Females y s 165.9 - .081 age .05 .21 .13 W EA Males y a 80.3 - .121 age 4» 1.20 SIL .02 .05 .89 <.001 ' EA Females y . 76.3 - .062 age + 1.16 SIL .02 .07 .80 <.001 ' Wanna EAMales y. 181.7-.10 age .04 .21 <.005 * mm 1! 246515959119. 49 -1° 41— ‘Regression equations significant at p50.05 SIL: subischial length . 146 TABLE 4.13 Results of ANCOVAs for differences in stature, skeletal dimensions, and AMA between EA and FN subiects, with age as the covariate. EA FN Limp n M n M F 9 Males 5:19.15 - Stature (cm) 75 142.3 25.5 22 150.4 24.0 0.84 .363 SSR ('16) 74 52.7 1 .9 22 52.2 1 .8 0.46 .501 Bicondylar (cm) 75 8.4 1.2 21 8.6 1.1 0.29 .590 Biepicondylar (cm) 75 5.8 0.9 22 6. 2 0.9 2.77 .099 Biacromial (cm) 74 32.6 6.1 22 35. 2 6.3 5.1 7 .025 ' Bicristal (cm) 74 23.7 4.6 21 24. 4 4.6 0. 01 .926 HSR (96) 74 72.6 3.8 21 70.3 3. 7 6. 34 .014 * AMA (crn’) 75 29.3 14.0 21 31.4 11.8 0. 04 .838 2015.15 Stature (cm) 179 175.5 7.3 35 175.8 5.6 0.29 .589 SSR (‘16) 179 52.3 1.5 35 52.6 2.0 0.71 .401 Bicondylar (cm) 177 9.8 0.7 35 10. 0 0.7 2.37 .125 Biepicondylar (cm) 179 7.3 0.5 35 7. 4 0.4 3.76 .054 ' Biacrcrrial (cm) 178 43.2 2.5 35 43. 9 2.5 0.79 .374 Bicristal (cm) 178 31.9 2.6 35 32. 5 2.4 3.43 .065 HSR (99) 177 73.8 5.1 35 74.1 4. 9 3.03 .083 AMA (cm’) 177 64.3 12.6 35 66.0 13. 6 0.30 .582 Females m Stature (cm) 60 142.1 21.8 19 149.4 17.3 0.79 .378 SSR (96) 60 52.8 1.4 18 53.1 1.5 1.29 .259 Bicondylar (cm) 59 8.0 1.1 18 8.0 0.6 0.09 .767 Biepicondylar (cm) 59 5.5 0.7 19 5.7 0.6 0.91 .344 Biacrorniai (cm) 59 32.4 4.8 19 34. 2 4.6 1.61 .209 Bicristal (cm) 59 24.2 4.5 19 25. 7 4.0 0. 76 .387 HSR 0%) 59 74.4 4.5 19 75. 0 4.1 0. 04 .846 AMA (cm') 59 26.2 1 0.8 17 27.2 7.0 0. 25 .619 2915116 Stature (cm) 1 80 161.9 5.9 54 162.8 5.6 0.12 .729 SSR ($6) 177 53.0 1 .4 54 52. 9 1 .3 1 .16 .282 Bicondylar (cm) 178 9.3 1.0 54 9. 6 1.0 7.04 .009 ' Biepicondylar (cm) 180 6.4 4.5 54 6. 6 0.5 17.45 < .001 ' Biacromial (cm) 178 38.2 2.0 54 39.5 1.8 16.94 < .001 ' Bicristal (cm) 179 31.1 2.9 54 32. 5 2. 8 17.72 <.001 ' HSR (‘16) 177 81.4 5.9 54 82. 3 5. 9 5.95 .016 ' 1 78 39.9 8.4 J1 40.3 7.1 1 .71 -199 'EA and FN samples significantly different at p50.05 147 TABLE 4.14 Results of ANCOVAs for differences in stature, skeletal dimensions, and AMA between males and females, with age as the covariate. 6:01.19 n Jean—SO n .Maan an I !L EA 5:195:16 Stature (cm) 75 142.3 25.5 60 142.1 21.8 5.18 .024 ' SSR (‘15) 74 52.7 1.9 60 52.8 1.4 0.59 .444 Bicondylar (cm) 75 8.4 1.2 59 8.0 1.1 15.73 <.001 ' Biepicondylar (cm) 75 5.8 0.9 59 5. 5 .7 33.14 <.001 ‘ Biacromial (cm) 74 32.6 6.1 ' 59 32. 4 4.8 5.81 .017 ‘ Bicristal (cm) 74 23.7 4.6 59 24. 2 4.5 0.04 .844 HSR (96) 74 72.6 3.8 59 74. 4 4. 5 5.82 .017 ' AMA (cm?) 75 29.3 14.0 59 26.2 10. 8 13.71 <.001 ' 212215.115 Stature (cm) 179 175.5 7.3 180 161.9 5.9 414.02 <.001 ' SSR (96) 179 52.3 1.5 177 53.0 1.4 21.90 <.001 " Bicondylar (cm) 177 9.8 0.7 178 9.3 1.0 41.19 <.001 ' . Biepicondylar (cm) 179 7.3 0.5 180 6. 4 4.5 414.89 <.001 " Biacromial (cm) 178 43.2 2.5 178 38. 2 2.0 451.87 <.001 " Bicristal(cm) 178 31.9 2.6 179 31.1 2.9 8.82 .003 ' HSR (96) 177 73.8 5.1 177 81.4 5.9 204.45 <.001 ' AMA(cm’) 177 64.3 12.6 178 38.9 8.4 498.59 <.001 ' FN 5:19.15 Stature (cm) 22 150.4 24.0 19 149.4 17.3 2. 31 .137 SSR (96) 22 52.2 1.8 18 53.1 1.5 3. 23 .081 Bicondylar(cm) 21 8.6 1.1 18 8.0 0.6 12.08 .001 ' Biepicondylar (cm) 22 6.2 0.9 19 5.7 0.6' 17. 24 <.001 ' Biacromial (cm) 22 35.2 6.3 1 9 34. 2 4.6 5.60 .023 ' Bicristal (cm) 21 24.4 4.6 19 25. 7 4.0 0.54 .465 HSR (99) 21 70.3 3.7 19 75. 0 4.1 13.65 .001 ‘ AMA (cm’) 21 31.4 11.8 17 27.2 7.0 13.15 .001 * 20:15:63 Stature (cm) 35 175.8 5.6 54 162.8 5.6 119.94 <.001 ' SSR (96) 35 52.6 2.0 54 52.9 1.3 0.46.498 Bicondylar (cm) 35 10.0 0.7 54 9.6 1.0 4.48.037 ' Biepicondylar (cm) 35 7.4 0.4 54 6. 6 0. 5 84.51 <. 001 " Biacromial (cm) 35 43.9 2.5 54 39. 5 1.8 93. 02 <. 001 * Bicristal (cm) 35 32.5 2.4 54 32.5 2. 8 0. 00 .990 HSR (96) 35 74.1 4.9 54 82.3 5. 9 4. 48 .037 " 'Male and female samples significantly different at p50.05 TABLE 4.15 148 Intraclass sibling correlations for stature, skeletal dimensions, circumferences, and AMA. EA FN _IQIAL_ r... F p r... F p r... F p Stature .66 2.91 <.001 ' .79 9.88 <.001 ‘ .53 3.70 <.001 " Sitting Height .64 2.76 <.001 " ‘ .51 4.52 <.001 ‘ .47 3.12 <.001 ‘ SSR .48 1.90 .001 ' .35 2.84 .001 ' .33 2.17 <.001 ’ SIL .58 2.38 <.001 ' .60 6.09 <.001 ' .46 3.05 <.001 ' Biacromial .58 2.39 <.001 ‘ .33 2.71 .002 ' .43 2.81 <.001 ' Bicristal .44 1.77 .003 ‘ .13 1.51 .11 .26 1.86 <.001 ‘ Bicondylar .52 2.06 <.001 " -.08 0.76 .78 .19 1.57 .006 " Biepicondylar .60 2.48 <.001 ' .14 1.54 .10 .34 2.36 <.001 ' Flexed Ann C. .53 2.10 <.001 ' .11 1.44 .14 .30 2.02 <.001 " RelaxedArmC. .54 2.15 <.001 ‘ .14 1.55 .10 .31 2.09 <.001 “ MaximaiCaifC. .59 2.46 <.001 ' .04 1.14 .35 .29 1.97 <.001 ' Waist C. .52 2.05 <.001 ‘ .25 1.59 .09 .36. 2.33 <.001 ' Hip C. .53 2.10 <.001 ‘ -.02 0.93 .58 .24 1.73 .001 " . AMA -36 1-54 ALL—Jaw 'Intraciass correlations significant at p50.05 149 TABLE 4.16 Interclass spousal and parent-offspring correlations for stature, skeletal dimensions, circumferences, and AMA. Father- Father- Father- Father-_ Mother- Mother- Mother- Mctbar .6911 W E A Number of Pairs 79 85 60 144 . 105 92 196 Stature .07 .36 " .43 ' .38 ‘ .29 ' .34 ‘ .31 ‘ Sitting Height -.06 .03 .20 .08 .24 ' .39 ‘ .31 ' SSR .08 .06 .14 .09 .23 ' .37 ‘ .29 " SIL .09 .38 ‘ .39 ‘ .38 ' .33 ' .35 ' .34 ‘ Biacromial .04 .11 .15 .13 .30‘ .37‘ .33‘ Bicristal .28 ‘ .13 .28 ‘ .21 " .19 ‘ .37 ' .27 " Bicondylar .05 .11 .22 .19 ‘ .38 ‘ .27 ' .32 ‘ Biepicondylar .05 .25 ' .28 ‘ .28 ‘ .25 ' .45 ' .34 " Flexed Ann C. .10 .21 .36 ' .27 " .31 ' .36 ‘ .12 Relaxed Ann C. .03 .19 .36 ‘ .26 ‘ .29 ‘ .38 ' .33 " Maximal Calf C. .05 .03 .38 ' .15 .24 ' .34 ' .29 ' Waist C. .13 .16 .17 .18' .23‘ .30‘ .26' Hb C. .14 .14 .37 ‘ .25 ' .35 ' .32 ‘ .32 ‘ AMA -.11 .10 .11 .11 .02 .26' .14 FN Number of Pairs 12 10 18 27 18 29 46 Stature -.09 .01 .00 .05 -.03 .51 ‘ .29 ' Sitting Height .04 .22 .12 .15 -.15 .29 .11 SSR -.06 .57 .45 .38 .81 ' .53 " .61 " SIL .13 .76 ‘ .32 .37 .68 " .65 ‘ .64 ‘ Biacromial .61 ‘ .56 .62 ‘ .52 ' .46 .53 ' .49 ' Bicristal .50 .38 .42 .40 ‘ .44 .34 .37 ' Bicondylar .27 -.22 .23 .19 .39 -.02 .1 1 Biepicondylar .75 ' .45 .41 .39 " .23 .23 .23 Flexed Arm C. -.18 -.17 34 .07 .07 .28 .21 Relaxed Ann C. -.20 -.17 34 .11 .64 ' ' .26 .43 ' Maximal Calf C. .26 -.34 48 .33 .13 .46 ' .39 ‘ Waist C. .10 -.12 48 .24 .25 .33 .32 ' Hp C. -.06 -.34 50 .27 .20 .25 .22 AMA .08 -.02 26 .17 .42 .31 .35 ' TOTAL Number of Pairs 91 94 77 170 122 120 241 Stature -.01 .16 35 ‘ .18 ‘ .24 ‘ .37 ‘ .30 ' Sitting Height -.05 .05 18 09 .20 ‘ .36 ' .28 ‘ SSR .06 .09 .20 .13 .33 " ' .40 ' .36 ‘ SIL .09 .36 " .38 " .37 ‘ .37 * .41 ‘ .38 ‘ Biacromial .11 .15 41 ' .24' .33‘ .42' 37‘ Bicristal .30 " .14 33 ‘ .24 ' .22 ‘ .38 ' 30 ' Bicondylar .07 .10 .24 ‘ 18 ' .39 ' .23 " .29 ‘ Biepicondylar .11 .25' 33' 29' .26' .42' 34' Flexed Arm C. .07 .14 37 " .23 ' .29 ' .34 ' 31 ' RelaxadArmC. .00 .13 38‘ .23' .35“ .36' 35' Maximal Calf C. .07 .01 43 " 17 " 23 " .37 ' .30 " Waist C. .13 .11 .28 " 18 ' .23 ' .37 ‘ .31 ‘ 1150. .12 .08 .42 ‘ .25? .34 ' .30 ' .30 " AMA -.10 .QL .22 .14 .07 .27 ' .17 ' 'Correiations significant at p50.05 150 TABLE 4.17 Heritability estimates for stature, skeletal dimensions, circumferences and AMA based on regression analyses of offspring on mid- parent values. J1 11’ S.E. 1 Significance— E A Stature 138 .68 .14 5.04 <.001” " Sitting Height 133 .34 .12 2.83 .005 ‘ SSR 132 .38 .1 1 3.43 <.001 ‘ SIL 133 .62 .09 6.83 <.001 ' Biacromial 134 .29 .09 3.05 .003 " Bicristal 134 .25 .08 2.99 .003 " Bicondylar 134 .27 .08 3.20 .002 ' Biepicondylar 136 .38 .09 4.05 <.001 ' Flexed Arm C. 136 .99 .08 13.05 <.001 ‘ Relaxed Ann C. 135 .36 .08 4.38 <.001 ' MaximaICalfC. 126 .33 .11 3.16 .002 ‘ Waist C. 1 33 .25 .08 3.09 .003 ' Hip C. 132 .35 .08 4.45 <.001 ‘ AMA 132 .08 .08 0.88 .38 FN Stature 17 .04 .13 0.35 .73 Sitting Height 15 .25 .30 .82 .42 SSR 15 .47 .31 1.53 .15 SIL 15 .37 .29 1.27 .22 Biacromial 16 .64 .19 3.38 .004 " Bicristal 15 .64 .32 2.03 .06 Bicondylar 15 .08 .58 .13 .90 Biepicondylar 16 .25 .29 .85 .41 Flexed Arm C. 13 .23 .44 0.52 .61 Relaxed Arm C. 14 1 .13 .45 2.49 .03 ‘ Maximal Calf C. 13 .38 .42 0.90 .38 Waist C. 13. .56 .35 1.60 .14 Hip C. 13 .86 .47 1.83 .09 AMA 12 .10 .35 0.28 .79 TOTAL Stature 155 .40 .10 4.00 <.001 ' Sitting Height 150 .32 .11 2.92 .004 ‘ SSR 149 .39 .1 1 3.67 <.001 ‘ SIL 150 .59 .09 6.71 <.001 ' Biacromial 152 .37 .09 4.27 <.001 ' Bicristal 151 .29 .08 3.62 <.001 ' Bicondylar 151 .25 .09 2.92 .004 ' Biepicondylar 154 .36 .09 4.07 <.001 " Flexed Arm C. 149 .35 .08 4.45 <.001 ‘ ' Relaxed Ann C. 151 .42 .08 4.94 <.001 ' Maximal Calf C. 142 .34 .10 3.37 .001 ‘ Waist C. 148 .27 .08 3.43 <.001 ' Hip c. 147 .39 .09 4.77 <.001 ' AM 146 .08 .09 (L92 .36 'Regressions significant at p$0.05 151 TABLE 4.18 Results of ANCOVAs for differences in body mass, fatness, and relative fat distribution between EA and FN sublects, with age as the covariate. EA FN Gimp fl Mean—SQ n Mean—ED J D Males 5:19.13 - Body Mass (kg) 75 40.6 21.0 21 46.9 21.0 3.39 .069 BMI (kg/m') 75 18.5 3.6 21 20.1 4.0 2.62 .109 TRUNK (mm) 73 30.4 20.6 21 48.1 28.8 10.96 .001 ‘ EXTREMITY (mm) 73 26.1 11.9 21 31.8 11.9 4.43 .038 " SUM (mm) 73 57.3 32.3 21 79.8 40.3 7.77 .006 ' TER(mmIn1m) 73 1.08 0.38 21 1.42 0.36 17.05 <.001 ' WHR (cm/cm) 75 0.87 0.06 21 0.90 0.05 7.28 .008 ‘ 29:25:16 Body Mass (kg) 178 83.6 1 5.5 35 87.2 13.6 2.28 .132 BMI (kg/m2) 178 27.2 4.7 35 28.2 3.8 3.65 .057 TRUNK (mm) 178 63.1 26.0 35 71.9 23.8 3.18 .076 EXTREMITY (mm) 175 27.7 1 1 .6 35 30.3 10.4 5.54 .019 ‘ SUM (mm) 178 91.7 38.2 35 102.1 32.6 3.45 .065 TER (mm/mm) 179 2.26 0.58 35 2.43 0.60 3.79 .053 " WHR (chcm) 177 0.92 0.06 35 0.93 0.05 7.25 .008 ' Females 5:19.115 Body Mass (kg) 60 40.5 20.3 19 47.2 21.7 1 .41 .240 BMI (kg/111’) 60 18.7 4.3 18 19.3 3.4 0.13 .725 TRUNK (mm) 57 37.2 24.3 17 44.5 21.0 1.95 .167 EXTREMITY (mm) 58 36.3 16.4 17 35.7 11.2 0.03 .871 SUM (rrm) 57 73.4 39.7 17 80.2 31.1 0.53 .467 TER (mm/mm) 57 0.98 0.29 17 1.22 0.26 8.1 5 .006 " WHR (cm/cm) 59 0.81 0.08 18 0.79 0.07 0.01 .917 Mills Body Mass (kg) 178 68.5 15.2 54 75.7 14.5 11.39 .001 ' BMI (kg/r11?) 178 26.1 5.5 54 28.6 5.4 11.98 .001 ' TRUNK (mm) 168 69.1 28.4 46 90.0 25.7 3.77 .053 " EXTREMITY (mm) 177 57.3 21.0 49 63.6 22.6 23.80 <.001 " SUM (mm) 166 126.0 47.3 45 152.5 45.6 13.84 <.001 ' TER (mm/mm) 168 1.23 0.36 45 1.47 0.35 21 .55 <.001 ' W 111 DJW; 'EA and FN samples significantly different at p50.05 152 TABLE 4.19 Results of ANCOVAs for differences in body mass, fatness, and relative fat distribution between males and females, with age as the covariate. __Mala___. ___Eamab.__ 6:099 n Mean—SD n Mean—SD F 9 EA 5.1.9.15 ~ BodyMass(kg) 75 40.6 21.0 60 40.5 20.3 2.06 .153 BM|(kg/m’) 75 19.5 3.6 60 19.7 4.3 0.24 .629 TRUNK (mm) 73 30.4 20.6 57 37.2 24.3 3.75 .055 EXTREMiTY(mm) 73 26.1 11.9 59 36.3 16.4 20.94 <.001' SUM(mm) 73 57.3 32.3 57 73.4 39.7 9.79 .004* TER(rm'iImm) 73 1.09 0.39 57 0.99 0.29 6.63 .011 * WHR(chcm) 75 0.97 0.06 59 0.91 0.09 24.96 <.001' 20.1515 BodyMass(kg) 179 83.6 15.5 179 69.5 15.2 101.71 <.001' BMI(kgIm') 179 27.2 4.7 179 26.1 5.5 7.44 .007* TRUNK (mm) 179 63.1 26.0 169 69.1 29.4 3.75 .054* EXTREMITY(rnm) 175 27.7 11.6 177 57.3 21.0 312.93 <.001‘ SUM (mm) 179 91.7 39.2 166 126.0 47.3 54.50 <.001' TER(mrnImm) 179 2.26 0.59 169 1.23 0.36 503.67 <.001' WHR(chcm) 177 0.92 0.06 179 0.79 0.06 559.13 <.001* FN 5:19.115 BodyMass (kg) 21 46.9 21.0 19 47.2 21.7 0.56 .459 BMl(kgIm’) 21 20.1 4.0 19 19.3 3.4 0.97 .357 TRUNK(mm) 21 49.1 29.9 17 44.5 21.0 0.03 .969 EXTREMi'iY(mrn) 21 31.9 11.9 17 35.7 11.2 1.26 .269 SUM(mm) 21 79.9 40.3 17 90.2 31.1 0.07 .790 TER(mm/mm) 21 1.42 0.36 17 1.22 0.26 4.66 .039* WHR(chcm) 21 0.90 0.05 19 0.79 0.07 29.17 <.001* 20:75.25 BodyMass (kg) 35 97.2 13.6 54 75.7 14.5 15.25 <.001* BMI(kgIm’) 35 29.2 3.9 54 29.6 5.4 0.03 .955 TRUNK(mrn) 35 71.9 23.9 46 90.0 25.7 76.00 <.001' EXTREMITY(mn) 35 30.3 10.4 49 63.6 22.6 10.02 .002' SUM(mrn) 35 102.1 32.9 45 152.5 45.6 29.79 <.001* TER(mmImm) 35 2.43 0.60 45 1.47 0.35 90.43 <.001* 166161901911) 35 0.9.3.4115 53 W 'Male and female samples significantly different at (150.05 153 TABLE 4.20 Intraclass sibling correlations for fatness, and relative fat distribution. LIL FN IQIAL r... F p r... F p r... F p 900me .57 2.29 <.001 ‘ .05 1.14 .346 .29 2.00 <.001 ' BMI .52 2.11 <.001 ' .06 1.16 .324 .28 1.94 <.001 ‘ SUM .46 1.82 .003 " .05 1.11 .375 .23 1.73 .001 " TRUNK .46 1.82 .003 ' .16 1.52 .113 .29 2.01 <. 001 ' EXTREMITY .40 1.66.008 ' - .11 0. 76 .779 .13 1.34.052 ' TER .57 2. 35 <. 001 ' .25 1.81.046 ‘ .38 2.51 <. 001 ' HUB 42 1 70 W991 ' -37 1.424.901; 'lntraclase correlations significant at ps0. 05 TABLE 4.21 Interclass spousal and parent-offspring for fatness and relative fat distribution. Father- Father- Father- Father- Mother- Mother- Mother- W E A Number of Pairs 79 85 60 144 105 92 196 Body Mass .08 .15 .38 ‘ .25 " .36 ‘ .39 ‘ .37 " BMI .01 .23 ' .36 ' .29 " .34 " .34 ' .34 ' SUM .07 .24 ' .25 .25 " .41 * .32 ' .36 ' TRUNK .10 .21 .22 .22 " .38 ' .29 ' .33 ' EXTREMIW .09 .32 ' .22 .28 ' .43 " .30 ' .35 ‘ TER .14 .33' .04 .23" .21' .15 .20 ‘ WHR .16 .08 -.14 .00 .10 .14 .12 FN Number of Pairs 12 10 18 27 18 29 46 Body Mass .06 -.22 .53 ' .15 .19 .36 .28 BMI -.04 -.20 .41 .06 .25 .09 .15 SUM -.10 -.46 .08 -.19 -.01 .22 .16 TRUNK -.08 -.36 -.23 -.28 -.01 .27 .20 EXTREMITY -.09 -.55 .57 " .17 .28 .18 .1 8 TER -.04 .00 -.22 -.06 .26 .10 .18 WHR -.18 -.10 .00 -.05 .52 ‘ .27 .35 ' TOTAL Number of Pairs 91 94 77 170 122 1 20 241 Body Mass .09 .10 .45 ' .24 " .34 ' .39 ' .36 ' BMI .01 .15 .40 ' .24 ‘ .33 " .31 ‘ .31 ‘ SUM .05 .17 .22 .19 ‘ .37 ' .32 ' .34 ‘ TRUNK .08 .13 .14 .14 .33' .33' .33‘ EXTREMITY .06 .25 ‘ .27 ' .25 ' .42 " .27 " .32 * TER .12 .27 " .00 .18 ‘ .21 ‘ .23 ' .21 ' WHR .11 .06 -.06 .03 .16 .39 ' 29 ' 'Correiations significant at p50.05 154 TABLE 4.22 Heritability estimates based on regression analyses of offspring on mid-parent values for fatness and relative fat distribution. n h’ SE- 1 Stamina—— E A Body Mass 138 .32 .11 3.03.003 ‘ BMI 137 .41 .08 4.91 <.001 ' SUM 120 .45 .10 4.44 <.001 " TRUNK 121 .36 .09 3.94 <.001 ' EXTREMITY 131 .45 .09 4.95 <.001 ‘ TER 122 .30 .11 2.78 .007 " WHR 134 .19 .14 1.32 .135 F N Body Mass 16 .11 .22 0.49 .639 BMI 16 .16 .26 0.61 .554 SUM 13 .01 .46 0.02 .983 TRUNK 13 .06 .38 -0.16 .879 EXTREMITY 13 .25 .53 0.47 .649 TER 13 .20 .19 1.03 .325 WHR 14 .04 .16 0.22 .829 TOTAL Body Mass 154 .26 .09 2.80 .006 ‘ BMI 153 .35 .08 4.50 <.001 ‘ SUM 133 .42 .10 4.28 <.001 ' TRUNK 134 .33 .09 3.76 <.001 ' EXTREMITY 144 .44 .09 4.82 <.001 " TER 135 .25 .09 2.75 .007 ' WE J48 .16 .11 1.50 .135 'Regressions significant at p50.05 155 TABLE 4.23 Prevalence of obesity. TSFandBMI TSFandBMl M ___in_mmblnatim__ __T_$E_ __BMI’__ ISEinx BMLQ'nI! ISELBMI N n % n % n % n % n % 5-19 yrs Males EA 75 17 22.7 16 21.3 3 4.0 2 2.7 14 18.7 FM 21 6 28.6 8 38.1 1 4.8 3 14.3 5 23.6 Eemales EA 59 11 18.6 10 16.9 5 8.5 4 6.8 6 10.2 FN 17 2 11.8 5 29.4 0 0.0 3 17.6 2 11.8 20-75 yrs Males EA 177 25 14.1 69 39.0 2 1.1 46 26.0 23 13.0 FM 35 6 17.1 18 51.4 2 5.7 14 40.0 4 11.4 Eemalas EA 177 53 29.9 62 35.0 7 4.0 16 9.0 46 26.0 EN 5] 25 41.1‘ 39 53.6‘ 2 3.9 8 15.7 22 43.1' ‘EA and FM groups significantly different in estimated prevalence at p50.05 (chi square) ‘TSF Obese defined as 2 85th percentile NHANES ll age- and sex-specific reference data in 5-19 yr old groups, and 2 85th percentile NHANES II for 18-24 yr old people: TSF 2 17.5 mm in males and TSF 2 29.5 mm in females in the 20-75 yr old groups aBMI Obese defined as 2 85th percentile NHANES ll age- and sex-specific reference data in the 5- 19 yr old groups and 2 85th percentile NHANES II for 20-29 yr old people: BMI 2 27.8 in males and BMI 2 27.3 in females in the 20-75 yr old groups TABLE 4.24 Differences between adult subjects classified as obese by different 156 criteria. Mann.— W Mean SD Mean 50 F 9 EA UALES n 46 23 Age. yrs 48.8 12.8 48.9 13.1 0.01 .970 Bicondylar Breadth, cm 10.2 0.6 10.6 0.8 8.69 .012 ' Biepicondylar Breadth. cm 7. 5 0. 4 7. 7 0.5 4.02 . .049 ' AMA. cm” 73. 7 12. 1 67.0 13.4 5.06 .028 ‘ WHR. cmlcm 0. 94 0. 04 0.97 0.05 9.18.004 ' TER, mnlnvn 2. 55 0. 59 1.97 0.32 20. 94 <. 001 ' Endomorphy 5.4 1.0 7.2 1.1 50.12 < .001 ' Mesomorphy 7.1 0.9 7.7 1.0 5.52 .022 ‘ Ectomorphy 0.4 0.3 0.2 0.2 6.25 .015 ' Overall Somatotype - - - - 16.44 <. 001 ’ EA FEIIALES n 18 46 Age. yrs 49.0 1 1.7 46.0 13.2 0.62 .433 Bicondylar Breadth. cm 9.7 0.9 10.3 1.0 5.23 .028 ' Biepicondylar Breadth. cm 6.6 ' 0.4 6.8 0.5 3.49 .067 MM. cm’ 43.7 9. 0 45.7 8.9 0. 63 .430 WHR, chcm 0. 82 0. 05 , 0.81 0. 05 0.10 .758 TER. mMnm 1.34 0. 28 1.24 0.19 2.30 .135 Endomorphy 7.2 1.2 8.5 0.8 22.38 <.001 ' Mesomorphy 6.4 1.4 7.2 1.4 4.80 .033 ' Ectomorphy 0.2 0.1 0.1 0.1 6.33.015 ‘ Overall Somatotype - - -- - 7.58 <. 001 ' EN MALES n 14 4 Age. yrs 44.1 13.9 41.2 15.9 0.13 .719 Bicondylar Breadth, cm 10. 2 0.5 10. 8 0.8 2. 79 .116 BiepicondylarBreadth.cm 7.5 0.5 7.8 0.4 0.93 .349 AMA, cm’ 751 12. 8 67.5 14. 3 1 .07 .318 WHR. chm 0. 95 0.04 0. 96 0.07 0.42 .526 TER. nun/run 2.80 0.40 2. 05 0.27 14.92 .002 ‘ Endomorphy 5.5 1.1 7.3 1.1 0.43 .520 Mesomorphy 7.2 1 .1 7.4 1 .0 2.08 .170 Ectomorphy 0.3 0.3 0.3 0.3 0.14 .712 Overall - - - - 2.28 .127 EN FEMALES n 8 22 Age. yrs 37.0 15.9 44.1 14.4 1.38 .250 Bicondylar Breadth. cm 9.7 0.7 10.2 1.0 1.80 .191 Biepicondylar Breadth. cm 6.6 0.4 6. 8 0.4 1.33 .259 AMA, cm’ 43. 2 4.1 41.4 6.9 0.30 .589 WHR, cmlcm 0. 85 0.03 0.66 0.05 0.00 .978 TER, min/inn 1.83 0.43 1.31 0.18 7.15 .013 " Endomorphy 7.8 0.6 8.8 0.7 12.18 .002 ‘ Mesomorphy 6.3 1.1 7.1 1.4 2.12 .159 Ectomorphy 0.1 0.1 0.1 0.1 0.02 .904 - - - - 3.23 ms; 'pS0.05 157 8de .3 see... 2.50553 8:23 2.. 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Egg .sam .6. 3.35:8 .55 .2... cup 3 82...... 5:828- .a..ea .o 2.3.: «.u 39:. 170 :8: .3823 83.8: $532.88. 52.39 A3: .383 52.58. 3 3 3 3 3 3 on 3 3 3 3 3 3 mm $6 3 3 3 3 3 3 83 3 3 3 3 3 3 3: $3.55; 3 3 3 3 3 3 v 3 3 3 3 3 3 e E 3 3 3 3 3 3 5 3 3 3 3 3 3 3m fined max—<2 2% «mm; 194 8882:: :5: :8 :5: :5: .882 8:: :8: .8: 8: a: H 2: 2:: 3:88: 8:2 .::::5 8:58 3:: 8:222 88:80 2 88.2 88.: E: u a :82: :88 8: 88.8: :8 28: 2.8 :3 83:2 :8 8 8:2 :8: :5. 852.: Eco» .flU< 8! ‘ssvw 3E mbéu mfiAEM—h mbén max—<2 msén and; Z..— «EU—Hfl UZE—m as Ins m ..8 u I nu nu -2 m I nu .m ..co :. m 1:: loo— 202 33522— .:8 :8 :5: :5: 82:2 8:: ::o: 6:: a: a: a 2: 2:: 8:88: .88: .:::::u 2:83 :8 5:35 5:: 8:222 8:::8u 3 9:22 :32: E: a 3 :82: :22: 0:: 88:2 :8 28: :3: § 8.8.2 mbéu wm—‘iiah Zr.— uEU—m—l QZE—m 204 SUBISCHIAL LENGTH: EA MALES 5-19 YEARS 1008 85" '80- 75" 70" SIL, cm 65" 55" 50‘ 45" 40 I I I I I I I r I 2 4 6 8 10 12 l4 16 18 20 AGE, years FIGURE 4.25 Estimated subischial length of EA males (0) 5-19 years and five-year age group means (A :l: SD) plotted relative'to U.S. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th peroentila. 205 SUBISCHIAL LENGTH: FN MALES 5-19 YEARS 100'- 954 85" . Q.— 808 ° 75" 70 " SIL, cm FIGURE 4.26 Estimated subischial length of FN males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to U.S. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :|: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 206 SUBISCHIAL LENGTH: EA FEMALES 5-19 YEARS 100- 95 - 85- 75" 70" SIL, cm 65" w- 55 ‘ 45 " AGE, years FIGURE 4.27 Estimated subischial length of EA females (0) 5—19 years and five- year age group means (A :i: SD) plotted relative to U.S. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :i: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 207 SUBISCHIAL LENGTH: FN FEMALES 5-19 YEARS 100- 95" 85" 75" SIL, cm 70". 55" 50- 45 "‘ AGE, years FIGURE 4.28 Estimated subischial length of FN females (0) 5-19 years and five- year age group means (A 1: SD) plotted relative to U.S. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are i SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 208 SSR: EA MALES 5-19 YEARS a) — O 57.5 8 55 ‘ B? 90‘ 0 V) 52.5 " 0 so .- 0 O o 475 T I I f I I I I I 2 4 6 8 10 12 14 16 18 20 AGE, years FIGURE 4.29 Sitting height/stature ratio of EA males (0) 5-19 years and five-year age group means (A i: SD) plotted relative to U.S. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 209 SSR: FN MALES 5-19 YEARS 57.5 ‘ 55" SSR, % 52.5 " 47.5 I I I I I 1 I r I AGE, years FIGURE 4.30 Sitting height/stature ratio of FN males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to U.S. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :|: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 210 SSR: EA FEMALES 5-19 YEARS 57.5 ‘ 55" SSR, % 52.5 "‘ 475 I I I I I I I I 2 4 6 8 10 12 14 16 18 AGE, years 8.: FIGURE 4.31 Sitting height/stature ratio of EA females (0) 5-19 years and five-year age group means (A :i: SD) plotted relative to U.S. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 211 SSR: FN FEMALES 5-19 YEARS 60-1 515- 55‘ SSR, % 515- 475 I I I I I I 2 4 6 8 10 12 14 16 18 20 AGE, years FIGURE 4.32 Sitting height/stamre ratio of FN females (0) 5-19 years and five- year age group means (A :l: SD) plotted relative to U.S. reference data (Hamill et al., 1973; Malina et al., 1974). Horizontal bars are :1: SD for“ age. Solid lines represent 10th, 50th, and 90th percentiles. 212 .8582& 58 us .58 .52 3323 .2: Bow an“ E 8 a as 35 3:88: .§§ .855 2a.»? as 5.88 as 8:52 56.86 a 2,32 Bee... 8:2» an a a 88.... 9.2» can .832 9a 5% 3.8.on 8.2. Va 5305 556 08332 swung 3 2,32 333 am a 3 «so... 9.2» a: .832 as 39» WEN § 83... z"— eo Se 233...»? ”55 3..“. go...— Eavm in}. 8 2. 2. 3 8 a... an we 9. . mm 8 3 8 n. b h — I «Q T—n 1 L INn O . o ”I‘lm flan .—. I o 0 Lo I3. I [A % ‘XSS mfl<fi> mhéu max—<2 Z..— ”mam 214 85822. 58 9a .53 .52 .8852 83. Bow a? 3. am a pa 3.. 3.38: .83 53.5 2a.»? ...a .286 as 8:32 5:350 2 832 Baa Em a a mace 82» own .833 e5 Ea mix § ease maéu mama—(SH..— mhén mflddfilflh 2h “mam Inn 75" 70" 65 - m: 55" 50‘ 45" AMA, cm 20- 15" 10" 216 AMA: EA MALES 5-19 YEARS FIGURE 4.37 Estimated arm muscle area of EA males (0) 5-19 years and five-year age group means (A :l: SD) plotted relative to U.S. reference data (Frisancho, 1990). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 75- 70- 65- AMA, cm ‘1‘ 201 15" 10" 217 AMA: FN MALES 5-19 YEARS I T I I I I T I 6 8 10 12 14 16 18 20 AGE, years FIGURE 4.38 Estimated arm muscle area of FN males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to U.S. reference data (Frisancho, 1990). Horizontal bars are 1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 218 AMA: EA FEMALES 5-19 YEARS 75- 70" 55" 50- 45 'l AMA,cm AGE, years FIGURE 4.39 Estimated arm muscle area of EA females (0) 5-19 years and five- year age group means (A :l: SD) plotted relative to U.S. reference data (Frisancho, 1990). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 75- 70" 65 " we: 55- AMA, cm 219 AMA: FN FEMALES 5-19 YEARS AGE, years FIGURE 4 .40 Estimated arm muscle area of FN females (0) 5-19 years and five- year age group means (A i SD) plotted relative to U. S. reference data (Frisancho, 1990). Horizontal bars are i SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 220 358209 58 use .58 £2 38952 8..: Bow a? é cm a pa 2.3 3:85: .892 283:6 as. 85.032 .3. a 932 Bean Em a 3 saga 95.» a: 5.39 e5 as» mien 63 8?: mhéu mania é ”<92 223 .8550qu 58 can .58 .52 888%.. 3:: 30m .0? Ba Gm H 8a E3 325.5: .253 65:3th 53 3:203“ .m.D 8 03.3.2 @333 am H 3 2808 use» can Roxana was an?» 338 A8 3380.. Zn— uo 3.3 28:8 8.3 3385mm 3... gnu—..— anh .HU< 9:.me mbéfl adi: 2h u§< la: TON— 224 BMI: EA MALES 5-19 YEARS 32" 24- BMI, kglm 18- 16- 14- 12 I I I fi r I I I I 2 4 6 8 10 12 14 16 18 20 ‘ AGE, years FIGURE 4.45 BMI of EA males (0) 5-19 years and five-year age group means (A :l: SD) plotted relative to U.S. reference data (Najjar and Rowland, 1987). Horizontal bars are :|: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 225 BMI: FN MALES 5-19 YEARS 32- 26+ 24" 22" BMI, kglm 20- 18" 16-1 144 12 FIGURE 4.46 BMI of FN males (0) 5-19 years and five-year age group means (A 1 SD) plotted relative to U.S. reference data (Najjar and Rowland, 1987). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 226 BMI: EA FEMALES 5-19 YEARS 32- 28" 26" 22- BMI, kg/m 18- 16- 14' - o 12 I I I I .l I I I I AGE, years FIGURE 4.47 BMI of EA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to U.S. reference data (N ajjar and Rowland, 1987). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 227 BMI: FN FEMALES 5-19 YEARS 32- 22- BMI, kg/m 18-' 16- 14" 12 I r I I I I I I I AGE, years FIGUREAAS BMI of FN females (0) 5-19 years and five-year age group means (A :l: SD) plotted relative to U.S. reference data (Najjar and Rowland, 1987). Horizontal bars are 1 SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 228 .8582»; 58 e5 .53 .52 882%. 8:: Bow .0? .8 am a 3 33 3:85: .992 €833. e5 amaze as 8:232 .mo 2 2,32 Baa Em « 3 was: 9.2» as 532 e8 38.» Pea § 838 mhén magma— <fi «an 231 3382:: so: :8 .58 .32 222:2 :22 2.8 .8: :2 a: H 2: 2:: 3:82.: 5:: .3233: :8 8.32: 3:: 8:222 .2: 2 8:22 :22: 8.: a 3 8:2: 2.2: 8: 88:2 :8 2:8 8.8 5 83:2 2: :o E: an... age:— Eaem dad: I8 InN mm ‘mm Inn I nv Ion ml<fl> nbén mfldéflh Z:— “Em 232 TRICEPS SKINF OLD: EA MALES 5-19 YEARS 35- 20'- 15" TRICEPS, mm 10" AGE, years FIGURE 4.53 Triceps skinfold of EA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to U.S. reference data (Najjar and Rowland, 1987). Horizontal bars are :|: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 233 35_ TRICEPS SKINFOLD: FN MALES 5-19 YEARS 15" TRICEPS, mm 10- FIGURE 4.54 Triceps skinfold of FN males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to U.S. reference data (Najjar and Rowland, 1987). Horizontal bars are :1: SD for age.’ Solid lines represent 10th, 50th, and 90th percentiles. 234 TRICEPS SKINFOLD: EA FEMALES 5-19 YEARS 35 - 20- 15" TRICEPS, mm 10"I AGE, years FIGURE 4.55 Triceps skinfold of EA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to U.S. reference data (N ajjar and Rowland, 1987). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 235 TRICEPS SKINFOLD: FN FEMALES 5-19 YEARS 35- so: 25- E 20- E a: U a 15- [... 10- 5- 0 I I I I I I I I I 2 4 6 8 10 12 14 16 18 20 AGE, years FIGURE 4.56 Triceps skinfold of FN females (0) 5-19 years and five-year age group means (A :|: SD) plotted relative to U.S. reference data (N ajjar and Rowland, 1987). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th. and 90th percentiles. 236 60:38.8:— 28 :8 .28 .22 222:2 8:: 2.2: a»: :2 am a 2: 2:: 3:85: $2 3.3:: :5 2:...an 2:: 8:222 2.: 2 3:22 :22: am 2 a :52: :82 um: 20:22 :5 28: 3.8 § :22: muéfl mfid mnén max—<2: Sm “Ga—OHZU—m EEO—Kn. 239 20350.8.“ 58 v5: .53 .52 88890.. 8..: 2.0m .ow: :8 Om H 2: was 3.82.25 A53 .93—39:— v.3 .33.qu 82.. 02.282 .2... 2 8.2.2 :82: E: 2 a 2:2: :22: 8: 28:2 :2 2:8 8...: .2 8222 z: 2 22:2: 88:: 8.: Eu... Each .HQ< 8 2. 2. n: 8 an an 9. 9. _ an 8 n: 8 n. p n P P p b p n P - n p b O In I... O I in In. Jfi L 0 I8 m 3 3 /.’ IWN u .lfll. .2. m 00 m Inn 0 o o In: Ion 932$ mhIeN 9.1—<2: 2h "DI—OhZ—v—m mam—OE 240 SUBSCAPULAR SKINFOLD: EA MALES 5-19 YEARS 5- 3o- 25- ° 0 O E E o e 20" fl 4 g o D 32 U 15" o .. 00 3 D O r]: o 7—0 i—l 10- ° 5- 0 I I I I I I I I I 2 4 6 8 10 12 14 16 18 20 AGE, years FIGURE 4.61 Subscapular skinfold of EA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to U.S. reference data (N ajjar and Rowland, 1987). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 241 SUBSCAPULAR SKINFOLD: FN MALES 5-19 YEARS 35" 30"! 0 ° 0 25- o g .. . 20.. ° ° 2: 4. A In] D a: < c U 15" m 5 A .__ .._., l-— —l m in ‘ 10" c 5- -——:.’ O T I I I I I I I I 2 4 6 8 10 12 14 16 18 20 AGE, years FIGURE 4.62 Subscapular skinfold of FN males (0) 5-19 years and five-year age group means (A :l: SD) plotted relative to U.S. reference data (N ajjar and Rowland, 1987). Horizontal bars are 1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 242 SUBSCAPULAR SKINFOLD: EA FEMALES 5-19 YEARS 35" O O 30- ° 25- E E 35 20- ..I D but 4 8 an 15- D VJ 10" 5- O I I I I I I I r I 2 4 6 8 10 12 14 16 18 20 AGE, years FIGURE 4.63 Subscapular skinfold of EA females (0) 5—19 years and five-year age group means (A :t: SD) plotted relative to U.S. reference data (Najjar and Rowland, 1987). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th. and 90th percentiles. 243 SUBSCAPULAR SKINFOLD: FN FEMALES 5-19 YEARS 35- 30— 25- E E “a 20- E A: 5 m 15- at: D m 10- AGE, years FIGURE 4.64 Subscapular skinfold of FN females (0) 5-19 years and five-year age group means (A 1: SD) plotted relative to U.S. reference data (Najjar and Rowland, 1987). Horizontal bars are :I: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 244 35580; 58 2a .58 .52 39.2.2 8.5 Bow and é am a pa 23 .5258: Age 6533— % amaze as. 8:232 do 2 2,32 383 Em a a 888 9.2» can sure. as as: 22 § 838 mhéu mums—<2 2h ”GAOhZ—Mm fi<flDm mbéN mfid<2flh «an "GAGE—255 m whéu mad; mhén max—(EH...— (fi al.—Olga Emu—U Gal—EGO 255 .8552»; 58 as .58 .52 some? 8:: Bow own .2 cm a as as 3.38: .62: «8.5 205.6 as 8:222 5685 9 2,32 88... Em a 3 .52.. 95.» om. auras 2a 28» 2.22 § mouse 2m do £2 + 5ch 5222 a.» Sansone 2.... ...—«:2..— anm .mu< on as as we cc mm on nv av mm on ma an n— . . p p . . . . . . h . . . an 13 |.. o o 1m, o ..o o... ooomnoo o o e -89 .. . Ta... 1T a. . m o o Tl‘lpdn T‘Ic H .— .m. m ..8 m on . o o o o X o 3 o N o 9 I8— 1 m. I. I8— 8 .16?— ..co. 24$ mbéu mas—<2?— Zh umhwzahm any—U final—ECU 256 TRUNK FLEXIBILITY: EA MALES 5-19 YEARS 4ST 35- FLEXIBILITY, cm 20 - 15- 10 I I I I I I I I I AGE, years FIGURE 4.77 Trunk flexibility of EA males (0) 5-19 years and five-year e group means (A :1: SD) plotted relative to Canadian reference data (Pimess Canada, 1 85). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 257 TRUNK FLEXIBILITY: F N MALES 5-19 YEARS 45 - 40- 35- FLEXIBILITY, cm 20 "‘ 0 0 154 10 I I r I I I I I I 8 AGE, years FIGURE 4.78 Trunk flexibility of FN males (0) 5-19 years and five-year age group means (A :I: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 258 TRUNK FLEXIBILITY: EA FEMALES 5-19 YEARS 45" 35" 25- o .- FLEXIBILITY, cm 20" 15" O 10 I l I I I I I l 1 FIGURE 4.79 Trunk flexibility of EA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 259 TRUNK FLEXIBILITY: F N FEMALES 5-19 YEARS 45 - 35- E o 1——A—1 9“ 3°“ ‘K/ o >" E‘ - 9—A—1 : E ,_, o x 25 II o g E o 20" 15 "l 10 T T I I I I I I I 2 4 6 8 10 12 14 16 18 20 AGE, years FIGURE 4.80 Trunk flexibility of FN females (0) 5-19 years and five-year age group means (A 2!: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 260 8035209 58 e8 .53 .52 888.8. 8..: 28m .88 .3 cm a as 83 3.588: .63 Sago .858 58 8:282 8:856 a 2,32 8,83 Em a 3 888 88» 88 .833 28 as: 2.8” 8V 838 an..." ”magma 4n.— uEA—m—Nfl: v—ZDE 263 .8588& 58 Ea .53 .52 382%. 8.5 Bow can .8 am a 93 as Beacon .69: £380 ”85% as. 8:20.». 535 3 2%? Baa am a a «.88. 9.2» can .833 as 23» 23m § 838 nbéu ”mu—<29.— Zh “FF—Ana—Nm—Ah MZDE 264 SIT-UPS: EA MALES 5-14 YEARS 55- 50" 45"! 35. SIT-UPS, nlmin 20‘ 15" 10" 5.1 00 O 4 6 8 1o 12 14 16 AGE, years FIGURE 4.85 Sit-ups in BA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 265 55 SIT-UPS: FN MALES 5-14 YEARS , 4s- 35‘ 30A SIT-UPS, n/min 20q 15" 10" FIGURE 4.86 Sit-ups in FN males (0) 5-19 years and five-year age group means (A :t SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :l: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 266 55_ SIT-UPS: EA FEMALES 5.14 YEARS 45 - 35" SIT-UPS, nlmin 20 - 15‘ 0 10' 4 6 8 10 12 14 16 AGE, years FIGURE 4.87 Sit-ups in BA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 267 55 SIT-UPS: F N FEMALES 5-14 YEARS 50" 45 "' SIT-UPS, n/min 20" 15- 10" AGE, years FIGURE 4.88 Sit-ups in FN females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Canadian reference data (Fitness Canada, 1985). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 268 FLEXED ARM HANG: EA MALES 5-14 YEARS 65- 55- 50" 451 HANG,s 20- 15" 10- FIGURE 4.89 Flexed arm hang in BA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 269 65- FLEXED ARM HANG: FN MALES 5-14 YEARS 55- 50- 45.. HANG, s 10" AGE, years FIGURE 4.90 Flexed arm hang in FN males (0) 5-19 years and five-year age group means (A 1 SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 270 FLEXED ARM HANG: EA FEMALES 5-14 YEARS HANG,s AGE, years FIGURE 4.91 Flexed arm hang in BA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubensu‘icker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 271 FLEXED ARM HANG: FN FEMALES 5-14 YEARS 65- 55" 504 45- HANG,s 20 " o 15- /— 1-—- —-1 10" 5‘ M400 l I I I AGE, years FIGURE 4.92 Flexed arm hang in FN females (o) 519 years and five-year age group means (A :t SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 272 DASH: EA MALES 5-14 YEARS 7J5“ 7-1 6.5 1 515- SPEED, m/s 445' 3:5- 3 I I I I I I 4 6 8 10 12 l4 l6 AGE, years FIGURE 4.93 135-meter dash speed in BA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 273 DASH: FN MALES 5-14 YEARS 8! 7.5 - 7d 6.5 '- SPEED, mls M I 4.5 "' 3.5 - 3 I I I I I l 4 6 8 10 12 14 16 AGE, years FIGURE 4.94 35-meter dash speed in FN males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 274 8_ DASH: EA FEMALES 5-14 YEARS 7.5 - SPEED, mls AGE, years FIGURE 4.95 3.5-meter dash speed in BA females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 8'! 7.5 " 7—1 6.5 ‘- 5.5 - SPEED, mls 5d 4.5 "‘ 3.5 '- 275 DASH: FN FEMALES 5-14 YEARS M - .. .. .. FIGURE 4.96 255-meter dash speed in FN females (0) 5-19 years and five- ear age group means (A 1 SD) plotted relative to Michigan State University Motor Pe ormancc Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 276 2.5 - JUMP: EA MALES 5-14 YEARS 2.25 e 2 —t 1.75 - E =3 1.5 - E D -1 1.25 . 1 -1 0.75 «- 0'5 I T I I I I 4 6 8 10 12 14 16 AGE, years FIGURE 4.97 Standing long jump in BA males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :|: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 277 JUMP: FN MALES 5-14 YEARS 2.5 '- 2.25 - 1.75 "‘ 1.5- JUMP, m 1.25‘ 0.75 "' 0-5 I I I I I 4 6 8 10 12 l4 l6 AGE, years FIGURE 4.98 Standing long jump in FN males (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 278 JUMP: EA FEMALES 5-14 YEARS 2.5 - 2.25 - 2-1 1.75 " 1.5-I JUMP, m 1.25“ [q 0.75 " 0-5 I I I I I I 4 6 8 10 12 14 16 AGE, years FIGURE 4.99 Standing long jump in BA females (0) 5-19 years and five-year age group means (A 1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker et al., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 279 JUMP: FN FEMALES 5-14 YEARS 2.5 '2 2.25 '1 1.75 "‘ ,m 1.5- 1.25- 0.75 " 0'5 I I I— I l I 4 6 8 10 12 14 16 AGE, years FIGURE 4.100 Standing long jump in FN females (0) 5-19 years and five-year age group means (A :1: SD) plotted relative to Michigan State University Motor Performance Study data (Haubenstricker etal., 1991). Horizontal bars are :1: SD for age. Solid lines represent 10th, 50th, and 90th percentiles. 280 Sign mafia...— 0325 05 .«o 5:88 05 3 2522 nose—n 9E < . nbén max—EH..— 35:. n56 I n6.— 1N finfi I nhfl tutu/mm ‘III'IJ. 284 Eases; 58 Ea 58 Sn 5823 8:: Bow .38. ..e .6 .865 as. :82". 2 2.32 Baa 2..— < . nbém mad; all?» 285 .iugenausasnénfifiaaaé 36m .39: ..e .6 Eat 5% =88". 2 332 Bee 2"— < ém 3 8.2.6. 8a as... «:3 9.8» emu 3.8... a... gen. Econ .an< we 2 no 8 n... 8 9. 3 ...n 8 3 8 n. - p I p p — h L p - - - n06 I rd I nhd I ad \¥III.I§.III?IIIIII I “NAU 4...... \|\ I G5 I had Ina/mo ‘arm m¢ nhéN ”Hagar.— "a”? 286 STATURE: MALES 5-19 YEARS 185- 180- 175- 170+ 165- 160- 155- 150- 145- 1404 135‘ STATURE, cm 1301 125- 120‘ 115'- 110‘ 105- 100- AGE, years FIGURE 5.7 Stature in BA Canadian males 5-19 years from studies ranging from 1953 to 1996. 287 .82 2 22 use wanna seem sea 3% 3-8 woes 5:850 nhéu mflflaflz "Hy—Dhaka 288 STATURE: FEMALES 5.19 YEARS 185- 180'- 175- 170-l 165' 160‘ 155- 150‘ 145 "' 140‘ 135- STATURE, cm 130- 125- 120'- 115‘ 110- 105‘ 100- 95 I r I I I I I I I 2 4 6 8 10 12 14 16 18 2O AGE, years FIGURE 5.9 Stature in BA Canadian females 5-19 years from studies ranging from '1953 to 1996. 289 .82 2 32 .55 was: 883... 88. ea...A 3.8 838a 8:85 nhén aux—<2: “Hagan—h nn— Ino— I2.— I n2 Inm— Ina ‘EIHHLVLS 290 MASS: MALES 5-19 YEARS 100' 80- 70" w:- 50" MASS, kg 30.1 20" ml 2 4 6 8 10 12 14 16 18 20 AGE, years FIGURE 5.11 Body mass in BA Canadian males 5-19 years from studies ranging from 1953 to 1996. 291 .82 2 22 5.5 was: 8:638 see as.» 38 8.8. 5:550 36 .88... IT 2 .2: 2.68. 9-22 I 8 n3. ....... 92m; nhéu £352 ”mm; 292 MASS: FEMALES 5-19 YEARS 80d 70" MASS, kg 30— 20 -' 10" FIGURE 5.13 Body mass in BA Canadian females 5-19 years from studies ranging from 1953 to 1996. 293 .83 3 Re 38.. usage 8:638 see as.» 3.8 Ease 5:380 E s was .68 .3 age: E3» .Hu< 8 n. 2. no 8 an on 9 3 an on a 8 2 _ — n P b n — r Ll - p h h h“ IS IS I2 r2. @3258... IT I8 .2: 1111111 I...» «SE: 111...... 22 1.1.1... 935; nu..." $343.2”: "was: I8 3! ‘ssvw 294 BMI: MALES 5-19 YEARS 26- 24— 22- 20 - BMI, kg/m AGE, years FIGURE 5.15 BMI in BA Canadian males 5-19 years from studies ranging from 1953 to 1996. 295 .82 a 22 59¢ wanna 8E3... Spa 5% 3.8 838 533.5