z . w r'h , r. a «mix 2. 9. gash.“ A J .av .iuisws. .nfl . . . . ”awnmwwwfiulv. .. ifiufiiwfifiw r .J urns; .Fs ;.. «1:: . EU,» .Mfi ”mm: . .f. 5.. 2H...£.. 3.4a . a. O. H} . I. .4. n... Lu , Ezafirmmw?! .e. 4 »z a»: 13.1.... ;. «W .i «.11? n: .g . .ry....1....u_ it 2.... . pa .Nnnhiu. , THESIS ATE UNIVERSITY LIBRAR llllHlUl'llIIHINIMHI | Ii ll 'lilllilililil 3 1293 01688 4755 This is to certify that the thesis entitled Big Fish In A Small Pond: Using Authentic Assessment In A Teaching Unit On Pond Fisheries Management presented by Eric W. Buhr has been accepted towards fulfillment of the requirements for Masters degree in Biological Science /(3 B-6 Name ENVIRONMENTAL CONSERVATION FISHERIES RESOURCES UNIT WEEK SEVEN: PRINCIPLES OF POND FISHERIES MANAGEMENT POST-TEST . Name 2 characteristics of a well-constructed farm pond and identify how each is important to maintaining a healthy fish population: What is stunting? Why does it occur? Identify 2 species commonly stocked in ponds that may become stunted, and identify 2 ways that a pond can be managed to prevent this fi'om occurring: What prey (food) fish should be stocked in a Michigan pond that you wish to stock bass in? WHY? Name 2 methods by which aquatic vegetation can be controlled in a farm pond: Identify and describe the 4 habitat zones of the pond and give 2 examples of organisms that can be found in each: What is bioaccumulation? What chemical contaminants may be found in farm pond fish? Name 2 ways that you can reduce your exposure to contaminants found in fish: 94 10. ll. 12. 13. 14. 15. Name 3 species of fish that should not be stocked in Michigan ponds: Diagram a fish scale labeling the focus, circuli and annuli: Describe how a fish’s age may be determined from a scale sample: If a first capture marks and releases 100 bluegills back into a pond and a second capture collects 100 bluegills, of which 10 were previously marked, what is the estimated bluegill population in the pond? Describe how a fish’s growth may be back-calculated using a scale sample and a ruler. Approximately how many years does it take for a bluegill in a farm pond to reach an “eating” size of 7-8 inches? For a largemouth bass to reach 12 inches? Name 3 reasons why people desire to have a pond on their property: Diagram a fish and label the following parts: operculum, anus, anal fin, caudal fin, pectoral fin, pelvic fin, dorsal fin, mouth, lateral line 95 B-7 GTOUPI QR_APP_.IE ENVIRONMENTAL CONSERVATION POND FISHERIES MANAGEMENT GROUP REPORT SCORING RUBRIC The following is the rubric that you will use to score the various portions group report during the peer review process. Please take the time to have your assistant and peer reviewer check your work against this form! Revision is best accomplished when you have some knowledge of where you need to go! Part One: Introduction (5 points) Score: 7“ Identify the pond’s: Format standards "" location + 12 point, double-spaced + owners :t default margins + age -'“ proper grammar 35 construction — no spelling errors + initial stocking + grade appropriate Part Two: Aquatic Life Survey (10 points) Score: 7 Identify and describe: '9' 4 pond habitats + aquatic vegetation (pictures) 4- macroinvertebrates (pictures) + fish species in pond to include: for each fish, identify: 4' largemouth bass 4" scientific name + bluegill - physical description -- pumpkinseed sunfish -- habitat preferences -- yellow perch - spawning information —- crappie +- picture Format standards 4" 12 point, double-spaced + default margins -- proper grammar -—— no spelling errors +- grade appropriate 96 Part Three: Fish Population Survey Report (10 points) Score 8 Describe: Format standards 4" capture methods/locations '4" 12 point, double-spaced + marking method + default margins + population estimate (formula) -- proper grammar - discussion of results — no spelling errors -' improvement of design? + grade appropriate Graph: 4" number and species of each fish + spreadsheet/chart __-I:_ color t title, subtitle, axes labels and legends + legible font and size Part Four: Age and Growth Survey Report (10 points) Score 8 Describe: Format standards — scale sampling method 4’ 12 point, double-spaced -— aging method :t default margins -- back-calculation of growth(formula) +- proper grammar ‘1' discussion of results :t no spelling errors t grade appropriate Graph: 4' age/growth for each fish by species «1" spreadsheet/chart j: color 1" title, subtitle, axes labels and legends + legible font and size Part Five: Pond Population and Growth Analysis (10 points) Score 8 Describe: Format standards 4’ Comparison of fish to standards + 12 point, double-spaced :1: Fish grth in pond j: default margins - Environmental factors +- proper grammar influencing growth 1: no spelling errors :1: grade appropriate 97 Part Six: Management Recommendations (5 points) Score 3 Discuss: Format standards "" results of study 4" 12 point, double-spaced -—- current status of pond fish 4— default margins population + proper grammar f“ review objectives 4’ no spelling errors -- recommendations for :t grade appropriate maintenance or improvement TOTAL SCORE: 38 ISO 98 Grant): EACH ENVIRONMENTAL CONSERVATION POND FISHERIES MANAGEMENT GROUP REPORT SCORING RUBRIC The following is the rubric that you will use to score the various portions group report during the peer review process. Please take the time to have your assistant and peer reviewer check your work against this form! Revision is best accomplished when you have some knowledge of where you need to go! Part One: Introduction (5 points) Score Identify the pond’s: 4' location + owners :1: age + construction 4" initial stocking Part Two: Aquatic Life Survey (10 points) Identify and describe: 4' 4 pond habitats -I- aquatic vegetation (pictures) + macroinvertebrates (pictures) 3!; fish species in pond to include: + largemouth bass 4' bluegill +- pumpkinseed sunfish 4" yellow perch -- crappie Format standards ‘i' 12 point, double-spaced + default margins 4' proper grammar + no spelling errors 3: grade appropriate 99 _$_’_ Format standards 7‘“ 12 point, double-spaced + default margins —- prOper grammar i no spelling errors 4' grade appropriate Score 7 for each fish, identify: "" scientific name 4’ physical description 4’ habitat preferences t spawning information 4' picture Score 7 Part Three: Fish Population Survey Report (10 points) Describe: Format standards 4' capture methods/locations + 12 point, double-spaced + marking method 4— default margins -- population estimate (formula) :I; proper grammar —— discussion of results + no spelling errors '— improvement of design? 1: grade appropriate Graph: ‘1' number and species of each fish 4' spreadsheet/chart 1: color _t title, subtitle, axes labels and legends ‘I' legible font and size _i. Part Four: Age and Growth Survey Report (10 points) Score Describe: Format standards 4' scale sampling method 4' 12 point, double-spaced j: aging method :1: default margins + back-calculation of growth(formula) + proper grammar :1: discussion of results 2!: no spelling errors :1: grade appropriate Graph: age/growth for each fish by species spreadsheet/chart color title, subtitle, axes labels and legends legible font and size Part Five: Pond Population and Growth Analysis (10 points) Score 8 Describe: Format standards + Comparison of fish to standards +’ 12 point, double-spaced 3‘; Fish growth in pond + default margins -- Environmental factors + proper grammar influencing grth 4— no spelling errors 3: grade appropriate 100 Part Six: Management Recommendations (5 points) Score 2 Discuss: Format standards "" results of study 4' 12 point, double-spaced + current status of pond fish 4' default margins population + proper grammar "’ review objectives :1; no spelling errors + recommendations for + grade appropriate maintenance or improvement TOTAL SCORE: V2- / so 101 APPENDIX C LABORATORY ACTIVITIES 102 C-l Name ENVIRONIVIENTAL CONSERVATION FISHERIES RESOURCES UNIT LABORATORY INVESTIGATION “HOW MANY FISH IN THE POND?” You made it! The trip through the pasture was a dangerous game of cat and mouse with a Holstein bull. Thankfirlly he decided to stop at the electric fence that gave you such a jolt! The water stretches out before you for several hundred yards. What a beautiful pond! By all appearances, it should be loaded with fish. But how can you find out how loaded it is? How do scientists estimate the number of fish in a pond? The populations of different fish species in a pond change on a daily basis. Reproduction, predation, and death from other environmental stresses all take their toll on the population. Certain age groups of fish, or year classes, have greater changes in their numbers than others. The young of the year (newly hatched “fry” and slightly larger “fingerlings”) that are serving as a food source for many other fish, birds, mammals, reptiles, amphibians, and even some invertebrates like predaceous diving beetles, will have numbers change every day. To assess the health of a pond’s fish population, it is necessary for the fisheries biologist to complete a population survey to determine the species distribution and numbers of fish in different year classes. Various methods may be employed to find the population size of a given species in the pond. First, if the fish are stocked, the total number of fish and their age going into the pond can be known precisely. Depending upon age, length and weight may also be determined prior to release so that accurate and detailed accounts of fish growth can be determined. In a pond with an existing population fish may be sampled by various means, marked and then a follow-up sample that finds previously marked fish can lead to an estimate of overall population size. This MARK-RECAPTURE SURVEY, as it is known, is widely used in the fisheries and wildlife sciences to give population estimates for a wide variety of species. Sampling methods used to capture fish often include netting by trap nets of seine nets, electric shocking, or drawing down the pond’s water level. In this lab activity you will be sampling the fish population in Feldpausch’s pond using recreational angling techniques, which will definitely influence the year class of fish that we are sampling. You will collect a scale sample fiom each fish caught so that we can find its age and mark the fish by clipping its pelvic fin prior to release. Next week we will take a second sample and see how many fish we can recapture, then estimate population size. For a successfirl capture/recapture survey of the fish population of F eldpausch’s pond, we must be careful in our handling of the fish that we capture, measure, mark and release. 103 Fish are protected fiom disease-causing organisms in the aquatic environment by a layer of mucus (not slime) that covers their scales. If this mucus coat is removed during handling, it may reduce the fish’s ability to resist infection by those diseases. For that purpose, do your best to 0 wet your hands prior to handling a fish 0 avoid dropping or dragging the fish on the ground 0 wet the surfaces of your measuring boards before use Fish exchange oxygen and carbon dioxide gas with the water through their gills. The gills are delicate structures that must remain moist to permit proper function. For that reason, do your best to keep the fish in the water (pond or pail) until ready to measure minimize time out of water for measuring process do not touch gills during handling if a fish is deeply hooked and damage to the gills may result from removal, cut the line at the fish’s mouth or see me for assistance To measure the total length of the fish, hold it flat against the horizontal board with its mouth closed and held up against the vertical board (wet boards prior to use). Pinch the tips of the caudal fin together to form a point and measure to the nearest 1/8 of an inch. Mark the fish by clipping off the bottom edge (5 V2”) of its pelvic fin. Release the fish by gently placing it to the water and allowing it to swim away. Avoid dropping or throwing the fish as it may stun it or cause internal damage. REMEMBER, THE SUCCESS OF OUR SAMPLING EFFORTS RESTS ON THE SURVIVAL OF THE FISH WE CATCH AND RELEASE. BE CAREFUL! Calculating the population size will require that you record the numbers of fish caught on our first trip, the number of fish caught on our second trip, and the number of fish that were previously marked out of those captured on our second trip. The following equation can be used to find the estimated population of each species that we catch: TOTAL MARKED TOTAL CAUGHT FIRST CAPTURE x SECOND CAPTURE TOTAL POPULATION = TOTAL RECAPTURES 104 Lab Questions: 1. What was the size range of the fish that were caught in the samples? What factors in our sampling techniques influenced the Size of the fish that were caught? Identify the species and number of fish that were marked in the first capture: Identify the species, number of fish and number of recaptures in the second capture. Use the formula above to estimate the population size of each species in the pond: How accurate do you think these estimates are? Name 3 things we could do to improve the accuracy of our estimates: What limitations are there on our ability to get a complete count on the pond’s fish population? EXPLAIN! 105 C-2 Name ENVIRONMENTAL CONSERVATION FISHERIES RESOURCES UNIT LABORATORY INVESTIGATION: “HOW OLD IS THAT FISH?” Wow! What a monster! The pond must’ve dropped a foot when you pulled that big old fish out of the water! You know you’ve got a big one, and you know it must be old, but just how old is it? How can you determine the age of a fish? Determining the age of fish is a very important skill for fisheries biologists to learn if they are to make accurate descriptions about the health of a pond’s fish population. Finding out how old fish are and then determining how fast they grow are key to understanding the environmental factors at work in the pond system. A pond that contains many large fish of average age is more productive than one that has few fish of the same size that are much older. The faster growth of the fish indicates that they are finding better conditions for their survival. Finding the age of the fish can allow the fisheries biologist to make recommendations on fish harvest and other actions that can Optimize fish production. Fish age can be determined in a variety of ways. Knowing the exact date of hatching of stocked fish that are put into the pond is the easiest of all. Finding the age of existing populations may be done by examination of scales, ear ossicles (bones), spines from fins, or other skeletal elements. The examination of ear ossicles, spines or bones requires some difficult work with stains and microscopes. A simpler method consists of examining scales under magnification, most easily accomplished by placing the scales on a microfiche projector. YEAH, THAT’S RIGHT, YOU GET TO GO MCRO-FICHING! Viewing the projected scale image allows you to see many concentric rings around a nearly central focus (the original birth scale) in the scale, much like the rings in a crosscut tree trunk. The difference between the two is that a fish scale’s many rings, or circuli, as they are properly known, are added to the scale throughout the year, unlike the annular rings on a tree. The scale’s rings become more densely packed together, however, during winter months when the fish’s growth rate slows. This packed region, or annulus, identifies the end of a year for the fish. Fish are hatched between April and August, and thus are not by calendar a full year of age when the first annulus is created. Fisheries scientists therefore established a rule that would bring conformity to the process of determining age. Fish are considered to be the age of their last identifiable annulus, regardless of the time of year collected. For each fish that you catch, a small scale sample must be collected and saved to be read in class using the microfiche projector. To take this sample, hold the fish firmly against the measuring board and scrape a small patch of scales fi'om its side between the 106 front edge of the anterior dorsal fin and the lateral line. Scrape in a tail to head direction for easiest removal. Do not oollect scales from the latoral line, as there are structurally different from what we need to look at. Label the collection packet with the necessary information (date, species, length, and location) and place the scales inside. Back in class, determine the age of each fish by examining the scale on the microfiche reader. Refer to the additional handouts to be sure that you do not have a lateral line scale (which shows an opening in the center) or a replacement scale (which shows a very large focus). Follow these guidelines to prepare and view the scale appropriately: Place a droplet of water on the lower glass plate of the projector about three- quarters of an inch in from the edge. Place one scale on the water droplet and close the viewing plates of the projector. Turn the projector on and slide the plates in until you can see the scale’s image. Center the image in the projector screen. Ifthe image is difficult to see because of air bubbles (little black circles or lines) then remove the scale and remount it in another droplet of water. Observe the image and identify the focus, then work outward and locate the densely packed circuli that denote each annulus. On old fish this may be more difficult due to the slower and less steady growth rates and changes that occur during spawning and other times of stress. Place a sheet of copy paper on the projector glass and then place a mark at the center of the focus. In a line from that point to the edge of the scale, place a mark at the location of each annulus and at the outer edge of the scale. Label the paper with the species and overall length. This page will be used in the next lab to calculate the fish’s growth at periods in its lifetime. Lab Questions: 1. What was the range in ages of the fish that you studied? 2. Compare length and age within species. Did fish of the same Species and age have nearly identical lengths? What could cause this? 3. How difficult was it to determine annuli on older fish (>5 years)? Why? 4. How confident are you in your ability to find the age of a fish using this method? 107 C-3 Name ENVIRONMENTAL CONSERVATION FISHERIES RESOURCES UNIT LABORATORY INVESTIGATION: “HOW BIG WAS THAT FISH WHEN. . . ?” You’re sitting in the boat with your 01’ Grandad, intently watching your rod tip for any Sign of a fish biting as the old codger rambles on with another one of his stories that begins with “When I was a kid just about your size...” when suddenly the monster of the lake nearly rips the rod from your hands! After what seems like hours later, you swing the gargantuan aboard and you and Gramps spend a few excited moments admiring the fish before snapping a few pictures and taking a few scales before releasing the behemoth to fight another day. The old fella snuffs and points at the scales you’re slipping into a collection envelope. “What’s that for, your trophy case?” he inquires. “Nope,” you reply. “I just want to find out how big this fish was ‘when I was a kid.”’ A very important part of the analysis of the pond’s fish population involves analysis of the growth rates of various fish species and the age classes within them. Fisheries scientists use a method known as back-calculation to determine the size of fish at various ages in its past based upon the information provided fiom a scale sample. The method utilizes the relationship between current scale length and current overall length of the fish to determine its length at any of its previous ages. The ratio between the current length of the scale and overall length of the fish at its present age can be used to calculate the fish’s length at a prior age according to its scale length at that age with the following formula: TOTAL LENGTH = TOTAL LENGTH x SCALE LENGTH AT AGE X AT AGE X SCALE LENGTH In this lab activity you will use the marked papers from the age determination lab to find the sampled fish’s length at all of its previous ages. To accomplish this, follow the instructions below: Measure the total scale length from its focus to the outside edge as marked on the page in the previous lab. Divide the fish’s total length as listed on the page by the length of the scale. Measure the distance from the focus to each annulus as marked on the page. 108 For each annulus, calculate the fish’s length at that age by multiplying the previous ratio of overall length /scale length and the scale length at that age. Write the fish’s length at each age in your notebook for use in constructing age and growth analysis graphs for the group project. Lab Questions: 1. Among bluegills of the same age, how did their sizes compare at Age 1? Age 2? Age 3? Give 2 reasons for these results: Among largemouth bass of the same age, how did their sizes compare at Age 1? Age 2? Age 3? Give 2 reasons for these results: If a nine-year-old, 42.50 cm (17”) largemouth bass has a projected scale length of 11.30 cm (4.5”), how long was the fish at age seven if the projected scale length is 10.60 cm (4.25”)? What does this indicate about the fish’s growth in the last two years? 109 APPENDIX D STUDENT INTERVIEW QUESTIONS 110 Name ENVIRONMENTAL CONSERVATION FISHERIES RESOURCES UNIT UNIT FOLLOW-UP STUDENT INTERVIEW QUESTIONS Please respond to each of the following questions as firlly as possible: 1. How could you determine the age of a fish that you caught? 2. How could you find out how long the fish was when it was 1 year younger? 3. If bluegills are stocked in a pond, what will eventually happen to their population? Why? How can this problem be avoided? 4. Ifbass and bluegills are in a pond together, what size must the bass be to be effective predators of the bluegill? 5. Ideally, what prey (food) fish should be stocked in a pond with bass? Why? 6. Name 3 species of fish that are not recommended for stocking in Michigan ponds: 7. Name 2 ways that the manner in which a pond is constructed can limit the amount of aquatic plant growth in it: 8. How do fish get contaminants in their bodies? 111 9. Where are most of the contaminants stored in a fish’s body? How can you reduce your exposure to them if you are going to eat fish? 10. Identify the difference between the benthic, littoral and limnetic zones in a pond: l 1. Draw and outline diagram of a fish and label the following parts: operculum, anus, anal fin, caudal fin, pectoral fin, pelvic fin, dorsal fin, mouth, lateral line 12. If someone asked you to estimate the size of a fish population in their pond, explain how you would go about it: 13. List three reasons why people desire ponds on their property: 112 D-2 Name ENVIRONMENTAL CONSERVATION FISHERIES RESOURCES UNIT UNIT FOLLOW-UP STUDENT QUESTIONNAIRE Please respond to each of the following as honestly and thoughtfully as possible: The following questions are about your knowledge before the unit was taught: _ . Could name more than 15 species of Michigan fish? 2. Could you identify more than 15 species of fish by their photograph? 3. Could you name the 4 distinct habitats of a pond and identify organisms that lived there? 4. Could you estimate the population size of a pond’s fish population? 5. Could you determine the age of a fish you had caught? 6. Could you determine how a fish had grown over its lifetime? 7. Could you identify the relationships between predator and prey fish in a pond and how their populations interact? 8. Could you identify the proper species and methods of stocking new ponds for fishing? 9. Had you filleted a fish? 10. Were you familiar with the advisory on fish consumption and how to best prepare fish to limit your exposure to contaminants? The following questions are about your knowledge after the unit: 11. Can you name more than 15 species of Michigan fish? 12. Can you identify more than 15 species of fish by their photograph? 13. Can you name the 4 distinct habitats of a pond and identify organisms that lived there? 14. Can you estimate the population size of a pond’s fish population? 15. Can you determine the age of a fish you had caught? 113 Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes No No No No No No No No No No No No No No No 16. Can you determine how a fish had grown over its lifetime? 17. Can you identify the relationships between predator and prey fish in a pond and how their populations interact? 18. Can you identify the proper species and methods of stocking new ponds for fishing? 19. Can you fillet a fish? 20. Are you familiar with the advisory on fish consumption and how to best prepare fish to limit your exposure to contaminants? Yes Yes Yes Yes Yes 2 O 2 O No No Below is a list of activities that we did during the unit. Please rate them fi'om 1 (strongly disagree) to 5 (strongly agree) for each of the following categories: Fish species identification using Michigan Fishing Guides Information was presented in a way that I could easily learn I learned the information well in the time spent on it I was motivated to learn this material I enjoyed this part of the unit and recommend keeping it Pond habitats and mural construction Information was presented in a way that I could easily learn I learned the information well in the time spent on it I was motivated to learn this material I enjoyed this part of the unit and recommend keeping it Fish population survey (mark/recapture lab) Information was presented in a way that I could easily learn I learned the information well in the time Spent on it I was motivated to learn this material I enjoyed this part of the unit and recommend keeping Determining fish age (scale reading lab) Information was presented in a way that I could easily learn I learned the information well in the time spent on it I was motivated to learn this material I enjoyed this part of the unit and recommend keeping it 114 Determining fish length at previous ages (back-calculated growth lab) Information was presented in a way that I could easily learn I learned the information well in the time spent on it I was motivated to learn this material I enjoyed this part of the unit and recommend keeping it The group report Instructions on what was required were clearly presented Workload was fairly distributed among all members by role assignment page Deadlines were fair Information needed to complete the report had been covered in class I was familiar with all information in my group’s report I peer reviewed those parts that I was assistant or reviewer for I learned more about report preparation I felt the report was worth more than just a grade because the Feldpausches were getting a copy Doing the report in a group was enjoyable and it Should be kept that way Please comment on what you liked most about this unit (less lecture, more labs, group work, working with Feldpausches, etc): Please comment on what you liked least about this unit (report work, labs, notes, etc): 115 BIBLIOGRAPHY 116 BIBLIOGRAPHY Alexander, R, Bahret, M.J., Chaves, J., Courts, G., and NS. 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