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Human Nutrition degree in Major professor Date 5'14'q :1” 0-7639 MS U is an Aflinnatt've Action/Equal Opportunity Institution TuanAnv Mlchl Unmfifi. PLACE IN RETURN BOX to remove this checkout from your record. TO AVOID FINES return on or before date due. DATE DUE DATE DUE DATE DUE 1M cJClm.p65-p.14 Development and validation of short instruments to assess dietary risk for low intake of fiber and readiness to change fiber consumption By Jen-Hon Collin Shih A THESIS Submitted to Michigan State University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Department of Food Science and Human Nutrition 1 997 ABSTRACT Development and validation of short instruments to assess dietary risk for low intake of fiber and readiness to change fiber consumption By Jen-Hon Collin Shih In community-based health promotion there is an emerging need for dietary assessment tools to rapidly screen for nutritional risk. At the same time, the application of behavioral change models, such as the Stages of Change Theory, to dietary intenrentions has yielded promising results to help researchers understand a broad range of problematic behaviors. This study addressed the development of instruments to screen for dietary risk of low fiber intake and to assess stage of readiness to increase fiber intake in a sample of 99 middle-aged, predominantly female clerical workers of Michigan State University. Results showed the test checklist had high sensitivity (0.80) for distinguishing subjects with low fiber intake, and moderate correlation (r=0.52, p<.01) with Zday food records. Subjects at Maintenance stage had higher average daily intake of fiber than those at Precontemplation and Action stages and received more social and environmental support than subjects at the other four stages. These findings: 1) support that the fiber checklist can feasibly identify a high risk population with low fiber intake; and 2) provide preliminary evidence that people at different stages of readiness for fiber consumption have somewhat different attitudes and beliefs. ACKNOWLEDGEMENTS I would like to give my hearty appreciation to Dr. Sharon Hoerr, for her patient and supportive instruction that guided me to the shrine of advanced intellectualism. Her thoughtful advice outside the classroom also broadened my perspectives on various issues that enriched my three-and-half- year “American experience”. Words are not enough to express how much her tutoring meant to me. Many thanks to my committee members, Dr. Ralph Levine and Dr. Won Song, for their heuristic assistance on thesis writing. Special thanks to my computer consultants here in MSU. Their altruistic help transformed me from a computer illiterate to an amateur troubleshooter, which enabled me to keep the indispensable computers in good shape. There will never be enough thanks to my parents. Their unconditional love and support were second to none in this universe. TABLE OF CONTENTS Ust of Tables vi Ust of Figures vii introduction 1 Research Objectives 4 Hypotheses 5 Glossary 5 Review of Related Uterature 7 Health implications and food sources of dietary fiber 8 Health implications of dietary fiber 8 Status and source of dietary fiber intake 10 The development of dietary fiber analyses 10 Remarks about literature reviewed 14 Factors associated with intake of dietary fiber 15 Determination of potential factors 15 Demographic factors 15 Personal knowledge 16 V Attitudes and beliefs - 16 Remarks about the literature reviewed 18 Modification of dietary behaviors using Stage of Change theory 19 Components of Stages of Change theory 19 The applicability of SCT in dietary behavior change 21 Remarks about the literature reviewed 25 The validity and reliability of dietary assessment instruments 26 Reliability and validity, the concepts 26 Methods of dietary assessment in community-level 26 programs Validation of new, short instruments in dietary assessment 29 Remarks about the literature reviewed 32 insight of the research from the reviewed literature 34 Methods 35 Subjects 35 Procedures 35 Instruments 37 Checklist 38 Stages of Change Questionnaire 40 Attitudes and Beliefs Questionnaire 42 Statistical analyses 43 Schedule 45 Results 46 Discussion 65 Checklist Validation 65 Stages of Change Questionnaire 67 Distribution of subjects across the stages of change 68 Attitudes and Beliefs Questionnaire 69 The rank of foods based on the quantitative contribution to total 70 fiber intake Strengths 71 Limitations 71 Conclusion 73 Bibliography 74 Appendices 87 Appendix A Permission from the University Committee on 87 Research involving Human Subjects (UCRIHS) Appendix B The brief explanatory letter sent to all 1,891 CTU 88 members Appendix C The consent form sent to the selected 401 CTU 89 members Appendix D The cover memo sent to subjects that didn't return 90 Appendix E The fiber checklist used in this study 91 Appendix F The Stage of Change questionnaire used in this study 92 Appendix G The Attitudes and Beliefs questionnaire used in this 93 study Table 1 Table 2 Table 3 Table 4 Table 5 Table 6 Table 7 Table 8 Table 9 Table 10 Table 11 Table 12 Table 13 Table 14 Table 15 Table 16 Table 17 Table 18 Table 19 Table 20 List of Tables Physiological effects and proposed mechanisms in vivo of dietary fiber Sources of five components of dietary fiber Recent studies measuring individual's dietary attitudes and behaviors Summary of various lengths of time for maintenance used for selected dietary behaviors in recent researches Studies that applied SCT in dietary behavior change Summary of studies developing new dietary instruments using a behavioral approach The sources and foods selected for the Checklist Staging questions and algorithm for dietary fat reduction used by Curry et al.,1992 Response rate and demographics of subjects Mean (iSD) of age, height, weight, and BMI of subjects by gender Average daily fiber intakes form checklist and 2-day food record and log transformed correlation The distribution of subjects [n=64) in high, medium, and low subgroups of fiber intake assessed by checklist and 2-day food record Fiber (g) assessed fiom the checklists and 2-day food records at each stage of change Spearman's rank order correlation between stage of change and estimated grams fiber intake from two instruments Mode, mean, and standard deviation of subjects' [n=95) responses to Attitudes and Beliefs questions The percentage of variance of responses to AB questionnaire for which each construct accounts Correlations of each question with five constructs and or of three constructs with more than one question The mode of responses to Attitudes and Beliefs questions and their distribution by five stages of change Mean ($313) of the scores for three major constructs at the subjects’ different stages of readiness to consume fiber Stepwise prediction of fiber intake from 2-day food record using fiber intake from checklist, the subject’s stage of change, and subjects scores of the three major constructs in AB quesitonnaire vi 11 17 21 24 30 39 41 46 46 50 52 54 54 56 57 58 59 60 61 Table 21 Table 22 Table 23 Table 24 Table 25 Ranking of foods from the checklist for their contribution to total fiber intake based on the average weekly number of portions consumed and grams of fiber consumed from that food per week by each subject Foods not listed on the checklist but were selected by subjects more than once Subjects' response to suggestions and barriers to increasing fiber intake Comparison of gram amount of dietary fiber intake (meaniSDj by stage of change between Shih’s checklist and data of Glanz et al., 1994; Spomy et ai.. 1995; and Huang et al., 1997 Distribution of subjects in five stages of change for fiber intake from several relevant studies 62 63 67 68 69 Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 6a Figure 6b Figure 6c Figure 6d Figure 7 List of Figures Diagram of Stages of Change Theory The evolution of the classification of dietary fiber components Change processes most useful in particular Stages of Change A section of eriett-type food frequency questionnaire A section of 13-item Block-type screening questionnaire for fat intake Distribution of fiber intake from checklist vs. 2-day food records Original distribution of fiber intake (9) from checklist Logarithmic distribution of fiber intake (g) from checklist Original distribution of fiber intake (9) from 2-day food records Logarithmic distribution of fiber intake (g) from 2-day food records Distribution of subjects in five stages of readiness to change fiber intake (n=99] [number of subjects are shown at the tip of bars) viii 28 29 47 48 49 49 53 General Introduction The importance of dietary fiber to health has emerged in the past two decades with advanced understanding of its role in human physiology. It has been well documented that low-fat, high-carbohydrate diets containing 20—35 g dietary fiber per day are beneficial for health promotion and disease risk reduction (ADA reports, 1993). Nevertheless, the US. public seems less concerned about fiber than about fat (Putnam, 1994) , even though aware ofthe linkage between cancer and insufficient amount of fiber in diet (Glanz et al., 1994). Americans’ dietary fiber intake has been reported as 11 g/day in NHANES II (Lanza et al., 1987) and 14.8 g/day in NHANES III (Alanio et al., 1994), both about halt‘the goal for dietary fiber for the Year 2000 (Healthy People 2000, 1990). Possible explanations for people's low intake of fiber have included: 1) unclear expectations regarding the diet- disease linkage (Smith et al., 1992; Smallwood et al., 1994); 2) difficulty in making dietary change (Kristal et al., 1990); and 3) lack of knowledge for selecting high-fiber foods (Levy et al., 1993; Smallwood et al., 1994). Assessing the dietary intake of free-living individuals remains among the most difficult measurement issues in epidemiological research and health promotion program evaluation (Kristal et al., 1990). The three generally accepted methods of dietary assessment—24-hour recalls, three-day food records, and food frequency questionnaires—— have been problematic for respondent burden and validity in community-level interventions where measurement must be rapid, simple, and inexpensive (Kristal et al., [990). In this research, we sought to develop instruments which could satisfy the need of community-level interventions with low respondent burden, while still maintaining acceptable validity and reliability. 7 Studies have shown that the efficacy of behavioral changes will be enhanced, if the intervention programs are based on appropriate psycho-theoretical models (Heimendinger ’ 1993; Glanz et al., 1994 ). A new theory, the Stages of Change Theory—originally Precontemplation I j Maintenance | I \‘ ontemplation ' Relapse I A] C / Action | \ j E Preparation | Figure 1. Diagram of Stages of Change Theory developed for intervention programs on smoking cessation and substance abuse management—is rapidly being applied to food consumption (Prochaska et al., 1985; Sandoval et al., 1994). The Stages of Change Theory proposes that change is a measurable process through which people progress to achieve a certain desirable behavior, such as smoking cessation (Prochaska et al., 1988; DiClemente et al., 1991) or reduction of dietary fat (Curry et al., 1992; Greene et al., 1994; Spomy et al., 1995). The process of change consists of precontemplation, contemplation, preparation, action, and maintenance stages. The dynamic, cyclic-like relationship of these stages is shown in Figure l (Prochaska et al., 1994; Sandoval et al., 1994; Si gman-Grant, 1996). The Stages of Change model can be beneficial for health professionals and program participants alike, because it allows demonstration of progress toward desirable change, even lapses, prior to actual change (Shelton, 1995), The early stages do not involve observable change of behaviors, but changes and attitudes or beliefs which can be measured. Lapses and relapses are anticipated as opportunities for improvement and not as absolute failure (Prochaska et al., 1994). Because 85% of the US. adult population is employed, the worksite setting offers immense potential for health promotion efforts (Morbidity and Mortality Weekly Report, 1993). The fact that six of the ten leading causes of death in the US. are linked to diet has persuaded the industries to provide worksite health promotion programs to their employees (Healthy People 2000, 1990; Sciacca, 1987). Chronic diseases such as heart disease, cancer, stroke, and liver cirrhosis have shown clear association with poor lifestyle factors, especially dietary habits. Knowing that these diseases represent tremendous costs of medical care and loss of working power, more and more business are aware that modifying behaviors which contribute to the development of debilitating conditions is more cost effective than treating established illnesses (Sciacca, 1987). The Surgeon General singled out nutritional habits as one of five categories as critical to the health and well-being of Americans and as targets for health promotion programs (US. Surgeon General, 1986). Many health risk factors for chronic disease are lifestyle behaviors which are alterable, and dozens of worksite programs have been exclusively designed to cope with them (Pritchard et al., 1990', Chenoweth, 1987). Programs to promote desirable eating habits are likely to be effective if based on an understanding of factors influencing food choices and at the stage at which the individual is (Sandoval et al., 1994; Laforge et al., 1994). Most interventions for dietary behavior change are designed for individuals at the action stage, while most people in the general population are at earlier stages (Glanz et al., 1993; Ockene et al., 1992). Theoretically, specific strategies should be planned for people at a specific stage of dietary change (Prochaska et al., 1994; Sandoval et al., 1994). So far few efforts have been made on applying the theory to increase dietary fiber intake (Glanz et al., 1994; Laforge et al., 1994). To sum up, the purpose of this research is to classify a sample population into different stages of change relative to their consumption of fiber and find out the characteristics of attitudes and beliefs for each stage, which we believe will improve the design of future nutrition intervention. Thus, in this research we will: 1) develop and test a short checklist foods to estimate subjects’ dietary fiber intake; 2) develop and test a stages of change questionnaire to categorize subjects into different stages for dietary fiber intake; and 3) develop and test a questionnaire to examine individual beliefs and attitudes about dietary fiber intake. The reasons follow for selecting the target population, members of Clerical Technical Worker’s Union (CT U). 1) The majority are mid-aged females who tend to be the main food providers in family, thus their preference of foods and diets can influence other household members, especially young dependents. 2) CTU’s are literate and have experienced questionnaire administration, allowing them to answer the test instruments without detailed interpretation by researchers. 3) An intramural health promotion program, MSU Healthy U, provides nutrition and health information routinely to its participants which are mainly female staff of MSU. The free dietary analysis offered is considered beneficial to the target population, because it is a costly health service. Another benefit for participants is that the importance of dietary fiber to health is reinforced. The results and instruments of this research will be available to the MSU Healthy U Committee to aid in future program design and evaluation. Research Objectives 1. Develop a short checklist of foods to assess individual fiber intake and validate it with concurrently collected 2-day food records from members of Clerical Technical Worker’s Union (CTU) of Michigan State University. 2. Develop a stage of change (SC) questionnaire for categorizing subjects into different stages by adapting the algorithm by Curry et al. 3. Develop a short questionnaire assessing individual attitudes and beliefs (AB questionnaire) related to dietary fiber by using components from Health Belief Model (HBM) that significantly correlate with dietary intake. 4. Determine the association offiber intake with the different stages related to dietary fiber by analyzing the checklist and stages of change questionnaire. 5. Determine the association of subjects’ attitudes and beliefs relative to dietary fiber intake and the stages at which they are. 6. Investigate the degree to which fiber intake can be predicted from fiber intake from checklist, stages of change, and the major constructs in AB questionnaire. Hypotheses —— . There will be positive correlation between the gram amounts of dietary fiber intake assessed from the checklist and from the 2-day food records. N . There will be no significant difference between the gram amounts of dietary fiber assessed from the checklist and the weighted 2-day food records. b) . The sensitivity of the checklist to detect high and low intake of dietary fiber will be _>_.7O when compared to weighted 2-day food records. A . There will be a significant difference between the gram amounts of dietary fiber intake for different stages of change related to dietary fiber. kit . Subjects’ responses to the attitudes and beliefs questionnaire will differ according to their different stages of readiness for fiber consumption. O\ . Subjects’ fiber intake from the weighted 2-day records can be predicted from 3 short instruments: the checklist, SC questionnaire, and the major constructs in the AB questionnaire. Glossary * Dietary fiber: the indigestible residue in food, composed of the carbohydrates cellulose, pectin, and hemicellulose; vegetable gums; and the noncarbohydrate lignin. (Dreher, 1987) * Precontemplation: the stage in which that the individuals have no intention to make a dietary behavior change (e. g., increase dietary fiber intake or cut fat intake), at least not within the next six months. (Glanz et al., 1990) * Contemplation: the stage in which that the individuals seriously think about changing a behavior within the next six months. (Glanz et al., 1990) * Preparation: the time that serious commitment to change has been made. For example, the individuals in this stage are those who tried to change their dietary behavior in the past year and who plan to do it within next month. (Glanz et al., 1990) "‘ Action: the span of time ranging from zero to six months after the initiation of overt change toward a desired dietary behavior. (Glanz et al., 1990) * Maintenance: the period beginning six months afier the action has been started and continuing until the desired behavior change has been adopted. (Glanz et al., 1990) * Criterion validity: the validity established on the agreement with a criterion measure or ”gold standard” which might be found either concurrent with the instrument being validated or in the future. (Patrick et al., 1991) * Content validity: the validity marked by the extent to which a measurement tool or instrument is consistent with a specific area of substantive content. (Patrick et al., 1991) * Face validity: the validity marked by the extent to which a measurement tool or instrument is pertinent to a specific area. (Patrick et al., 1991) * Predisposing factors: the factors affecting a specific dietary behavior utilization including: 1)belief in the diet-disease connection; 2)perceived benefits of healthy diet; and 3)knowledge about a healthy diet. (Glanz et al., 1993) * Enabling factors: the factors affecting health services utilization including: 1)perceived barriers to healthy diet; 2)socia1 support; and 3)perceived norms for healthy eating. (Glanz et al., 1993) Review of Related Literature This chapter reviews the literature related to the key concepts of this research. The literature review is divided into four sections: 1) health implications and food sources of dietary fiber; 2) factors associated with intake of dietary fiber; 3) modification of dietary behaviors using Stages of Change Theory; 4) the validity and reliability of dietary assessment instruments. Health implications and food sources of dietary fiber Health imglications of dietary fiber Three decades ago Burkitt and Trowell hypothesized that dietary fiber intake played a role in the etiology of colon cancer and other diseases (Burkitt et al., 1975) and stimulated subsequent research. Although interpretation is clouded in some case-control studies by confounding dietary parameters and the absence of reliable information on fiber content (Block et al., 1987), the role of dietary fiber has been promoted by nutrition and medical researches from simply a laxation facilitator to a potential preventive of several chronic diseases, such as diverticular disease (Brodribb, 1980), colorectal cancer (MacLennan et al., 1978, Modan et al., 1975; Kaaks et al., 1995), Type II diabetes (Comi et al., 1995; Anderson, 1985; Manhire et al., 1981; Nuttall et al., 1979), prostate cancer (Ross et al., 1990; Weisbruger, 1987), hypertension (Margetts et al., 1988; Schlamowitz et a1, 1987; Wright et al., 1979), and hypercholesterolemia (Jenkin et al., 1995 ; Jenkin et al.,1980; Jenkin et al., 1979). Despite the variety of health benefits associated with dietary fiber, however, many of the mechanisms in vivo remain unclear (Eastwood, 1992). Table 1 summarizes the physiological effects of dietary fiber and proposed mechanisms. Some of the health benefits of dietary fiber components might be in part its ability to bind water and other factors such as bile acids and estrogens (Chenoweth et al., 1976; Key et al., 1979; Lindegarde et al., 1984). Other benefits might come from the increased transit time of fecal bulk, colonic fermentation of soluble fiber to butyric acid and slowed absorption of glucose. When fiber intake is increased, water and fluids must be increased as well to avoid digestive problems (ADA Reports, 1988). 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Q... 55 5. 5... .58 m85 @5855 555.55. 888.5: 5:5 8955 85852.5. 58.5 .55.. 5:5: 55: 38.... I852. 5. 5....58 .:5 58.5 5555.. 5:5 @588 55:58.. 5 85:58. 55.5. . 95:95.: 5.... 55.55. 555.5. 585.85 5:5 55.55 5555 58.555: 3 .:5 52:85.8: <88. 5. 5... .85 5. 855.5 .55. 55: .5 8,5 588 8.55:8 5:: .:5 58.5 55:5..55. >358: 5. 5... .8. $55.. 5: .:5 553.55 5. :5: 5.8.. 258:5 5585:5255 5.. :5: 552.: 5:55.588: .:5 5.5:. 5. 5... .me 38.5.5535 5.555 5. .55: 55: 8:85:55: 855. 5.85 25585. 255.55 58.5 58:55.5: 5:: 5:5:55 =5 855.5: 5. 5.5555: 5:5 5555555855. 1555. 5. 5... .53 5:5: 5 85 55 5.55. 5.5558 5 .53. Status and source at dictum: trber intake Nutritionists, food and supplement manufacturers, and health promoters have made intensive efforts to spread the word about the virtues of dietary fiber, however, intakes remain far below the levels recommended by US. Department of Agriculture (USDA), the US. Department of Health and Human Service (DHHS), and other health authorities (Smallwood et al., 1994). The third National Health And Nutrition Examination Survey (NHANES III) revealed the mean and standard error of dietary fiber intake was 17.001027 grams for males and 12.755019. grams for females (Alaimo et al., 1994), while the recommended daily intake is 20 to 30 grams by the National Cancer Institute (NCI) (Pilch, 1987) or 25 grams per 2,000 kcal diet by Foods and Drugs Administration (FDA) on food labels (FDA, 1993). Surprisingly, data from USDA’s 1989-90 Diet and Health Knowledge Survey (DHKS) and its associated Continuing Survey of Food Intakes by Individuals (CSFII) revealed that 51% of 2,880 respondents thought their diets were “about right” in fiber and those who thought they should lower their fiber intake consumed only 7.3 grams per day (Smallwood et al., 1994). These findings imply an need for nutrition education to educate people about their actual consumption of dietary fiber as well as the rich sources of dietary fiber and its benefits to health. The develo men! 0 dieta tber anal ses Dietary fiber comes only from plant components and was first defined by Hipsley (Hipsley, 1953) to describe the plant cell wall components of food including insoluble lignin, cellulose, and hemicellulose. The definition of dietary fiber has been broadened by some to include soluble substances such as pectins, gums, and mucilages (Trowell, 1976). Table 2 summarizes the general sources of dietary fiber. This current broad definition acknowledges the significance of fiber as a chemical and physiological component of the diet. As a result of findings from medical research on the health benefits of fiber, the public policy on dietary fiber has been modified in the past three decades. Based on the definition ll that fiber is ‘the insoluble material remaining after severe acid and base hydrolysis’, the US. Department of Agriculture (USDA) Handbook 8 provided the first comprehensive data on the amount of crude fiber in foods in 1963. As fiber analysis methods advanced, USDA released version 4 of the USDA nutrient database in November, 1990, which included both soluble and insoluble dietary fiber values of foods (USDA, 1990). Because this database is machine-readable, it is widely used for individual food intake surveys. In January, 1993, Food and Drug Administration (FDA) and USDA issued final food-labeling regulations including nutrition labeling and health claims for dietary fiber (Lee et al., 1995). However, there is no level set for dietary fiber in the current Recommended Dietary Allowances (National Research Council, l989). Table 2 Sources of five components of dietary fiber Cellulose Wholewheat flour Wax beans Bran Broccoli Cabbage Brussels sprouts Young peas Cucumber Skins Green beans Peppers Apples Carrots Hem/celluloses Bran Cereals Whole grains Mustard greens Brussels smouls Beet root Gums Oat meals Rolledoat products Dn‘ed beans lignln Breakfast cereals Eggplant Bran Pears Older vegetables Green beans Strawberries Radishes Pectin Squash Cabbage Apples Dried peas Citrus fruits Carrots Cauliflower Strawberries Green beans Potatoes The sophisticated structures and physico—chemical properties of dietary fiber components have provoked much argument about how they should be classified. The l2 advance of analytical methods for these components and better understanding of their physiological effects on health has helped the development of an unanimous classification system. In 1995, an international survey was conducted to get the views of 147 professionals in the field on the definition of dietary fiber (Lee et al., 1995). The survey also solicited opinions on analytical methods for nutrition research. Figure 2 shows the most updated classification system of dietary fiber components and its evolution. 1_l Non-starch Non-cellulose Gum and Other 833:" F°°d 9 m Polysaccharidee Polysaccharides 9 ”"53““ ' Polysaochaides Dietary Fiber Mes Pectin Henioellulose Cellulose Lignin Enzyme- Resistant Starch ,Degradable. l Starch l l l ‘The solubility of some components depends upon the method of analysis used. Thus, some of the pectin fraction may be treated as “insoluble”, or a pan of hemicellulose may be treated as “ soluble". Figure 2. The evolution of the classification of dietary fiber components (ILSI, 1994) 13 Two types of analytical methods, gravimetric methods and the Southgate method, are commonly used to determine dietary fiber content of foods (Asp et al., 1992). The gravimetric methods, used over 130 years ago, weighed the insoluble residue (crude fiber) after extraction with boiling dilute acid, alkali and enzymes. Gravimetric methods can provide results varying from the amount of total fiber to its fractionated components, depending on the degree of sophistication of extraction. Soluble fiber is not included in the results of gravimetric methods, because the intensive extraction severely damages fiber structure. Some proteins and minerals may be in the residue which can falsely increase the amount of total fiber (Asp, 1995). To correct these analysis problems, a frequently performed method uses alcohol or enzymes to precipitate soluble fiber before the intensive extraction (Selvendran et al., 1984). The enzymatic-gravimetric methods were named the most practical and simplest ways to determine major components identified as dietary fiber (Prosky et al., 1984; Lee et al., 1995) and adopted by the Association of Official Analytical Chemists (AOAC) as Official Methods 985.29, 991.43, 992.16 (AOAC, 1990). The second type of methods to analyze dietary fiber content devised by Southgate (Southgate, 1982) were originally used to provide data for the food composition tables in the United Kingdom. These methods employed more sophisticated extraction than the gravimetric methods, therefore, the results have more detailed chemical information about the fractions of both insoluble and soluble fiber, especially low molecular weight polysaccharides (Asp et al., 1992). The most updated Southgate methods use automated gas-liquid or high performance liquid chromatography for separating sugars after hydrolysis of the fractionated fiber components. Each type of method—gravimetric, and Southgate, has strengths and limitations, so there is no best choice for every analytical attempt. Gravimetric methods usually suffice where information on dietary fiber is required for regulatory purposes. If the research emphasizes on the nature and physiological properties of dietary fiber polysaccharides, however, Southgate methods are more feasible and preferred (Asp, 1995; Asp et al., 1992). AA [4 Fiber is rich in foods such as whole grains, fruits, vegetables, and legumes (Anderson et al., 1994). A recent report stated most Americans fail to consume even one whole-grain food per day, much less than three, which might explain why the average daily intake of fiber is less than half of the recommended value (Alberton et al., 1995). From the 24-hour recalled dietary intakes of the NHANES 11 survey, Block and colleagues discovered the major single contributors of dietary fiber in the US. diet were vegetables, which provided 28% of daily fiber, breads provided 19% and fruits 7% (Block et al., 1987). Similar findings reported by Marlett and colleagues indicated that one third of the daily fiber intake of 200 college students was from grain products and legumes (Marlett et al., 1981). A secondary analysis on specific food sources of fiber using data from the 1985 CSFII by Krebs-Smith and colleagues found that tomatoes, yeast breads, potatoes, green beans, soy products, and French fries contributed about 40% of total dietary fiber in diets of 1,459 women 19 through 50 years old (Krebs-Smith et al., 1992). The same analysis also revealed that subjects received an average of 42% of their dietary fiber from cereal and bakery products and 28% from vegetables and potatoes (Smallwood et al., 1994). In the Bogalusa Heart Study, conducted from 1976-1988 on three different age groups of 2,1 1 8 children and young adults, Nicklas and colleagues reported that vegetables, soups, breads, and grains accounted for 53% and 70% of the total fiber in the diets of 10- and 13-years- olds, respectively (Nicklas et al., 1995). Remarks about literature reviewed Over the last 30 years, the role of dietary fiber in nutrition has been changed from a “non-nutritive ingredient” to a factor “representative of healthy diet” as analytical methods and experimental dietary studies have progressed. Much has been written concerning the advantages of a high-fiber diet on various chronic diseases, however, the average daily intake of Americans still falls far behind the recommended value and people appear largely unaware of what foods are good source of fiber. 61 AA 15 Factors associated with intake of dietary fiber In the past two decades increasing concern and knowledge about the role of diet in the etiology of chronic disease have led to the development of dietary guidelines by health authorities in US. and other industrial countries. One major component these guidelines share is the emphasis on reduced fat intake and increased fiber consumption (National Research Council, 1989). To achieve the goals of these dietary guidelines, such as an increase in average consumption of fiber to 20-30 grams per day suggested by National Cancer Institute, an understanding of factors that strongly influence individual’s food choice is indispensable for the development of intervention programs. Determination of gotential (actors A broad range of determinants contributing to dietary behavior has been recognized, including intrapersonal, interpersonal, institutional, community, and public policy influences (Glanz et al., 1990). Research examining dietary behavior has tended to concentrate on personal factors such as nutrition knowledge (Johnson et al., 1985); health and diet-related beliefs (Jensen et al., 1992); confidence of making dietary changes (Vega et al., 1988); and feelings of personal control over dietary behavior (Saltzer et al., 1978). Once potential determinants of specific dietary behavior are identified, these can provide a foundation for developing effective strategies for dietary change (Glanz et al., 1993). Additionally, measuring these variables might make it possible to evaluate change in mediating factors, in addition to the major endpoints, of food consumption (Kristal et al., l990). Demographic factors: Studies have found low socioeconomic status associated with lower dietary fiber intake (Baghurst et al., 1990; Smith et al., 1992', Kushi et al., 1988). Patterson and colleagues reported that people who had low-fat, high-fiber diets tended to be older women with high incomes and college education (Patterson et al., 1994). Specific dietary preference is another significant factor influencing dietary fiber intake. Vegetarians l6 typically consume a high levels of fiber—perhaps 40 grams or more daily—compared to non-vegetarians, while the historically carnivorous Eskimos eat virtually no fiber at all (Eastwood et al., 1983). Personal knowledge: Correlated with low socioeconomic status, lack of knowledge may be another factor affecting fiber intake. A study using a telephone survey of foodservice specialists in 242 military installations found only 24% of the respondents, who nearly all perceived that they offered a whole-grain bread, actually did. Findings indicated brand names, misleading labels, and lack of knowledge in identifying real fiber-rich foods were associated with low fiber intake (Warber et al., 1996). In a telephone survey on 407 adults residing in Providence, RI, Laforge and colleagues used psychosocial approaches to probe for factors associated with low fruit and vegetable consumption (Laforge et al., 1994). They found that education was directly related to fruit and vegetable intake, but was not correlated with those who usually consumed fewer than 5 servings and had no intention of adopting the national S-A-Day program. Studies done by other researchers (Bunch, 1990; Subar et al., 1992) also confirmed that low educational level is likely to be associated with low fiber intake. Animdgs and beliefs: Based on data from 1985 CSFII and 1989-90 DHKS, another psychosocial factor influencing dietary fiber consumption proposed by Frazao and Cleveland was the individual’s assessment of the adequacy of his or her own intake (Frazao et al., 1994). They found people who regarded themselves as consuming enough dietary fiber in the diet, actually ate less dietary fiber than those who thought their fiber intake was not enough, even though both groups fell well below the recommended daily values for dietary fiber. To acquire quantitative data for assessing abstract dietary attitudes and beliefs, numerous researchers have generated questions using concepts from the Health Belief Model (HBM) (Contento et al., 1990; Dittus et al., 1995; Patterson et al., 1995; Kristal et al., 1995; Trenkner et al., 1990). The HBM is one ofthe most commonly applied models in nutrition education (Contento, 1995) and a major organizing framework for explaining and .- l7 predicting acceptance of health recommendations (Glanz et al., 1990). For the concept of improving disease-related dietary behavior, the HBM would suggest that behavior is influenced by a readiness to take action and intervening beliefs about the behavior. Readiness to take action related to health could result from the perceived susceptibility to a disease state or concern about nutrition (Hayes et al., 1987; Dittus et al., 1995). The key components of the HBM are: l) perceived threat; 2) outcome expectation; and 3) self- efficacy (Glanz et al., 1990). A list of recent studies using IBM and other psychosocial theories to measure individual’s dietary attitudes and beliefs is tabulated in Table 3. Table 3 Recent studies measuring indiviglral's dietary attitudes and behaviors Researchers Subjects Dietary behavior Psychosocial model(s) used in investiga_ted designing Questionnaire Cotugna et al., 22,043 adults cancer prevention diets Not specified 1992 nationwide Dittus et al., 1995 1,069 Washington fruits and vegetable HBM State residents intake Contento et al., 117 adult comprehensive, positive HBM 1990 supermarket dietary changes Theory of Reasoned Action sho rs Health Locus 'of Control Smith et al., 1995 487 adult 12345+ Food and HBM Australians Nutrition Plan diet Stages of Change Health Locus of Control Kristal et al., 16,287 healthful diets including HBM 1995 participants of increase fiber, fruits Stages of Change Working Well and vegetable intake Social Learning Theory Trial and decrease fat Diffusion of Innovation intake Social Supgrt Theory Remgrks about the literature reviewed Aside from the traditional advocacy focusing solely on the health virtues of fiber, an understanding of the psychological, socioeconomic, and other potential attitudinal factors that influence intake of dietary fiber may be helpful for nutrition educators and health promoters to screen for those with low intake and design effective interventions. The concepts of the HBM offer comprehensive perspectives on individual’s perceived barriers and motivations for desired dietary change. However, for dietary fiber, more efforts are needed to clarify the contribution of these psychosocial factors to tangible change of dietary behaviors such as attitudes, beliefs, and self-efficacy. The research does suggest that some of these psychosocial factors might predict those at risk for low intakes of dietary fiber. 19 Modification of dietary behaviors using Stage of Change theory Comgonems ot Stages 0t Change theory Stages of Change theory (SCT) was developed by Prochaska, Diclemente and colleagues in the early eighties (Prochaska et al., 1982; McConnaughy et al., 1983) in an attempt to understand how and why people make health related changes. They proposed that change occurs through a series of stages and processes shown in Figure 3. Piece/item lat/on Contem lation Pre rat/“on Action Ma/htenance Consciousness-Raising _ Social Liberation Emotional Arousal Self-Reevaluation Commitment Reward Countering Environment Control Helping Relationships Figure 3. Change processes most useful in particular Stages of Change (Prochaska et al., 1992; Prochaska et al., 1994) I A' The definitions of these stages are: r ‘ r or not thinking about making a change; contemplation—seriously thinking about changing; preparation—— making definite plans to change; action—actively modifying an undesired behavior; and ' ‘ ' ' U the new, favorable behavior for certain length of time which might be from three months to one or two year. Figure 3 also shows the types of intervention efforts thought to be appropriate at each stage to lead people to the next stage of readiness to change. A4 20 The SCT initially was designed for psychotherapy intervention programs such as smoking cessation (Prochaska et al., 1983; Prochaska et al., 1985; Prochaska et al., 1988; DiClemente etal., 1991); alcohol intake reduction (DiClemente et al., 1990; Norcross et al., 1991 ); adoption of physical activity (Marcus et al., 1992); and prevention of coronary heart disease (Ockene et al., 1990). Some claim that the SCT might be an integrative theme for understanding and accelerating change in a wide range of problem behaviors, including dietary ones (Prochaska et al., 1992). Several researches have applied SCT to promote fat intake reduction (Curry et al., 1992; Greene et al., 1994; Spomy et al., 1995); cholesterol reduction (Southard et al., 1992); fiber intake increase (Brinberg et al., 1990); and adoption of healthy diets (Glanz et al., 1994). Broader applications have used SCT for weight management (O'Connell et al., 1988; Prochaska et al., 1992), which is not really a behavior but requires a group of behavioral changes in diet and exercise patterns and social support, to achieve optimal weight. In SCT, stages link to one another in a dynamic, linear-like pattern. Each stage, however, does not inevitably lead to the next one, because it is possible for the individual to move back to the previous stages or leave the system entirely (Prochaska et al., 1992). For this reason, some researchers have questioned the use of the term “Stage” to refer to levels of readiness to change wherein individuals can regress (personal communication). In physiology, for example, a stage refers to an immutable progression of development towards maturity (Rhoades and Pflanzer, 1992). Perhaps “state of change” would be more appropriate term to use than “stage of change” for this model. In the maintenance stage, there is no a priori reason for a particular time span for a particular dietary behavior and this is an area which could benefit from research. Researchers customarily have decided the length of time span for the maintenance stage based on that in previously related studies (Spomy et al., 1995). The time lengths used for maintaining desired dietary behaviors in some related studies are listed in Table 4 and vary from “made the change” to “longer than 2 years". “Longer than 6 months” has been the length of time used most frequently and by researchers funded by National Cancer Institute to study intake of fat and fruits and vegetables (Glanz et al., 1994). 49 A4 21 Table 4 Summary of various lengths of time for maintenance used for selected dietary behaviors in recent researches Desiregdie_tarxbehavior Time length for maintenance Source Low-fat and high-fruit and presently trying Campbell et al., 1994 vegetable diet Low-fat diet longer than 6 months Cuny et al., 1992 Low-fat and high-fiber diet longer than 6 months Glanz et al., 1994 Low-fat diet longer than 6 months Greene et al., 1995 Seal/$353 frurts and longer than 6 months Laforge et al., 1994 Overall dietary change already made change Smith et al., 1995 Low-fat and high-fiber diet longer than 2 years Spomy et al., 1995 The agglicabilitv of SC T in dietary behavior change Because most diet-related health problems develop gradually and do not present immediate symptoms, to be effective, programs promoting healthy dietary behaviors must reach large segments of the population and influence the diverse factors which determine eating patterns (Glanz et al., 1988). The first use of SCT in programs promoting healthy eating habits was regarded as a conceptual and methodological challenge (Glanz et al., 1993). Glanz and Mullis explained that the attempt to clearly and correctly categorize a person into a specific stage for dietary behavior change required attention to the unique characteristics of eating patterns and practical issues about the eating environment which were barely researched at that time (Glanz et al., 1988). Subsequently, some algorithms and instruments were tested and validated for their effectiveness on categorizing individuals into different stages of behavioral change (Curry et al., 1992; Glanz et al., 1994). A4 22 For healthy people of normal weight, most diet-related health problems do not present immediate or dramatic symptoms, and are not the targets of social stigma compared to alcohol or drug abuse or obesity. Without a provocative psychological reasons, most healthy people are not motivated to make dietary behavior change, even if their diets are inadequate (Glanz et al., 1994). Researchers have found that traditional health education messages and action-oriented interventions may be counterproductive for people at the Precontemplation stage, because people in this stage are highly resistant to change and do not believe any change is necessary or that their current behavior threatens their well-being (Ockene et al., 1992; Ershoff et al., 1989). Because the SCT underscores the need and strategies to move from precontemplation to contemplation, its concepts offer individuals promise of future change in dietary behaviors (Brownell et al., 1995) and are considered as effective ways to decrease early dropouts (Prochaska et al., 1986). A few studies have reported the applicability of the SCT used in promoting dietary change in healthy populations. A summary of several reviewed studies is in Table 5. Curry and colleagues assessed stage of dietary fat reduction and its association with fat intake using telephone interviews with two predominantly white samples in Washington state. They found consistent results across the two samples indicating more women than men in action and maintenance stages, and significant associations between stage of readiness for dietary fat reduction and percent of calories from fat (Curry et al., 1992). Rossi and colleagues evaluated distributions for four different staging algorithms for dietary fat reduction and compared the findings across three samples in New England using in person, mail, and telephone methods. The distributions differed between the algorithms and, with all four algorithms, dietary fat consumption levels decreased with successive stages of change (Rossi et al., 1993). Glanz and colleagues assessed the psychological stages of change in relation to both dietary fat and fiber intake on two samples— predominantly middle-aged, white males— using food frequency and questionnaires about psychosocial factors. Findings indicated that a greater proportion of the population had actively tried to reduce fat intake than to consume more fiber; and intake of fiber and vegetables was significantly lower in Precontemplation, Contemplation, and Preparation stages than that in Action and AA 23 Maintenance stages (Glanz et al., 1994). In a national 5-A-Day campaign, Laforge and colleagues studied 405 adult respondents to investigate psychosocial factors related to fruit and vegetable consumption using the SCT. Results show that education was positively related to fruit and vegetable intake and inversely related to being in the Precontemplation stage. Males were twice as likely as females to be in the Precontemplation stage and eat fewer than 2 servings of fruits and vegetables a day. Such findings imply that stage of readiness to change should be considered as well as other factors in planning interventions for increasing fruit and vegetable consumption. 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Food checklists do not exist specifically for fiber. Also, one might question whether a correlation coefficient of0.50 is adequate to establish validity, or whether it is even the appropriate technique for validation of such tools. Perhaps the sensitivity, or ability ofa tool to detect who is at dietary risk would be more meaningful for establishing the validity of a rapid community-level screening tool. 34 Insight of the research from the revlewed literature Knowing that increased dietary fiber intake helps reduce the risks of several chronic diseases and peoples’ average daily intake is still far below the recommended amounts, more efforts on promoting the public awareness and recognition of benefits of fiber seem necessary. Nutrition interventions combining the information of what changes to make and how to make changes might be useful to achieve this goal. The thesis research serves as a prologue for the development of such intervention. Validating a short checklist of foods for fiber intake examines the feasibility ofa rapid method to collect dietary intake data, or a food component of interest. Developing a SCT flowchart provides a simple way to stratify subjects into different stages of readiness for different intervention strategies. Composing a questionnaire of attitudes and beliefs toward dietary fiber intake reveals the characteristics of subjects at difierent stages, which might be used as intervention points in program design as well as to validate the SCT paradigm for dietary change. The aggregation of such instruments as these three might be an integral piece for screening subjects at risk for low intake of dietary fiber before the onset of core intervention, and providing baseline information for evaluating the efficacy of intervention. 35 Methods Subjects Subjects were the 99 respondents from a random sample of 401 adults selected from the members of Clerical Technical Worker’s Union (CTU) of Michigan State University (MSU), currently 1,891 members. The average age for this population was 42 years old and nearly 92% of them are female. The name list and mailing labels for subjects were obtained for $25.00 from MSU Human Resource Information Data Service in September, 1996. The reasons follow for selecting this target population. 1) The majority of CTU’s are mid-aged females who tend to be the main food providers in family, thus their preference of foods and diets can influence other household members, especially young dependents. 2) CTU’s are literate and have experienced questionnaire administration, allowing them to answer the test instruments without detailed interpretation by researchers. 3) An intramural health promotion program, MSU Healthy U, provides nutrition and health information routinely to its participants which are mainly female staff of MSU. We expected the research to be mutually beneficial to CTUs (increased awareness of dietary fiber) and Healthy U program (provide information for future program design and screening those at risk for low intake). Procedures Data collection began after permission from the University Committee on Research Involving Human Subjects (UCRIHS) (Appendix A) and the MSU Healthy U Committee. A pilot test was conducted on the secretaries (n=10) of Food Science and Human Nutrition (FSHN) Department after the permission from chairperson of FSHN was obtained. Preliminary support from the MSU Clerical Technical Worker’s Union (CTU) was sought, and at first granted, by the President of the CTU. Unfortunately afier all questionnaires 36 were developed and prepared, the CTU President found, after extensive discussions with her advisory board, that the CTU could not officially support the survey with a letter of recommendation. This was because the Union was initiating formal health-benefit contract negotiations with the university. Eventually, the test instruments were mailed without the support letter from the CTU President. A brief explanatory letter was sent to all 1,891 CTU members regarding the purpose of the research before the sample selection occurred (Appendix B). The sample of 401 people received the consent form. (Appendix C), the test instruments, and an incentive. The test instruments, which include one food checklist, one Stages of Change (SC) questionnaire, a one attitudes and beliefs (AB) questionnaire and food record forms for one weekday and one weekend day, were mailed to selected subjects in October, 1996. As an incentive, one dollar coupon to the campus Dairy Store and an offer of free dietary analysis was provided. Interested subjects provided their age, height, weight, and exercise pattern for a free dietary assessment. The test instruments were self- administered with brief directions. It took about 10 minutes to complete the food checklist and two questionnaires. More time, about 30 minutes, was needed for recording 2-day’s food intake. Due to the poor response rate (16%) for this first mailing, a second mailing of test instruments was mailed again with a cover memo (Appendix D) to subjects that did not return. The final response rate reached 25%. All survey responses were collected by researchers and kept in a file cabinet to ensure confidentiality of subjects. The fiber content of foods on the checklist was extracted from the MSU NutriGuide 2.0 program (Song W.O., Nutritional Analysis Computer Program, Michigan State University, 1990) and entered into an [BM-compatible computer using Microsofi Excel 7.0 program (Microsoft Inc., Seattle, WA, 1995). Results served as the database to calculate subjects’ fiber intake assessed by the checklist. To judge the validity of the test checklist, results were compared to the fiber intake computed from the concurrently-collected 2-day food records analyzed by using MSU NutriGuide 2.0. MSU NutriGuide analyzes diets for 27 nutrients in 953 different foods. 37 Ninety-nine percent of the foods in the software are complete for fiber composition. Its nutrient composition data originally came from Michigan State University main-frame database and USDA Handbook-8 (USDA, 1990). However, sources like food manufacturer’s information, McCance and Widdowson (Holland et al., 1991), and Pennington (Pennington, 1989) were also included in the database. Before the primary data collection on CTUs, both the checklist and the questionnaires were pilot-tested on all secretaries in the Food Science and Human Nutrition Department (n=10). The draft test instruments were modified slightly based on response to the pilot test. In the checklist, not-selected food items were omitted, such as cowpeas. Standard portion size was added to each food for better accuracy in estimating actual consumption. In the Stages of Change questionnaire, a slight change in wording was made to include people already on a high-fiber diet in the maintenance stage. For the attitudes and beliefs questionnaire, one question was eliminated because of similar wording to another question. Two open-ended questions were added to acquire subjects’ detailed description of motivation and barriers for increasing dietary fiber intake. The summarized responses will be made available to Healthy U for improving future program design. Instruments One checklist of food sources of fiber and two questionnaires related to changing dietary behavior related to fiber were developed for administration by mail. It was hypothesized that these three short instruments, taking less than 10 minutes to complete would be able to identify people at risk for low intake of fiber as well as the more lengthy 2-day food records. 38 (fhecklist The purpose of developing the checklist was to establish a valid tool for ranking people by their dietary fiber intake. Establishing criterion validity of this checklist was one of this study’s goals. The 2-day food records from Healthy U participants in three previous projects were used to develop a list of food items contributing to 95% of the fiber intake in participants’ diets. The computer program written in Turbo Pascal (Borland International Inc., Scott Valley, CA, 1987) language developed by Huang and colleagues (Huang et al., 1994) was used to accomplish the sorting job. The “top 40” foods contributing fiber were assumed to reflect the fiber consumption of our target population. The amount of dietary fiber intake was determined by the following formula: Fiber (g/day) = Quantity of serving X Frequency (daily) X Fiber content of the food (g) Further selection of food items for the checklist were from two sources: 1) the food sources of dietary fiber in diets of 1,032 women 19 through 50 years old in 1985 Continuing Survey of Food Intakes of Individuals (CSFII) data, which lists the food items contributing to 70% of dietary fiber resources (Krebs-Smith et al., 1992); 2) the dietary checklist formed by Kristal and colleagues, because it has fair criterion validity (F 0.57) with two 4.day food records and one Block-type FF Q and fair test-retest reliability (weighted Kappa= 0.48) (Kristal et al., 1990; Laforge et al., 1994). See Table 7 for sources of foods in the checklist developed for this study. The final edition of the checklist used in this study (Appendix E) contained 47 foods divided into 7 groups: Fruits, Vegetables, Beans/Legumes, Breads/Grains/Pasta, Nuts, Snack foods, and Cereals. A few foods were kept on the checklist which did not come from the sources in Table 7, because these foods might be “indicator” foods for people who consume high fiber diets. A blank for one food item in each group was also included to allow subjects to add foods not on the checklist. A standard portion size, closest to usually consumed amounts, was selected for use on the checklist to keep the format concise. -The 39 small, medium, and large serving sizes in the Block-type FFQ were not included, because studies suggested the variations in food intake are mainly determined by frequency of consumption rather than by portion sizes (Samet et al., 1984). Also, subjects tend to pay very little attention to the serving size information on FFQ without the presence of instructor (Smith et al., 1991; Subar et al., 1995). Table 7 The sources and foods selected for the Checklist Diets of Healthy U CSFII data, 1985 Dietary checklist by Fiber-rich, participants by Huang et al., Kristal et al.,1990 ‘lndicator' 1994 foods apple apple baked beans berries bagel broccoli broccoli celery banana corn brown rice granola bran flakes crackers Brussels sprouts grape broccoli French fries cauliflower nectarine carrots green beans kidney beans peach French fries lettuce lima beans popcorn green beans lima beans melon spinach green peas noodles orange noodles pasta/spaghetti peanut butter oat bran peanut butter peanuts oatmeal peanuts potato chips orange potato pmnes pasta/spaghetti potato chips raisins peanut butter ready-to-eat cereals ready-to—eat cereals pear tomato walnuts pizza tortillas whole-wheat bread potato white bread zucchini potato chips whole-wheat bread prunes raisin bran raisins tomato tortillas tossed salad white bread whole-wheat bread 40 Stages 0t Change Questionnaire One Stages of Change (SC) questionnaire was designed to categorize subjects into one distinct stage of readiness to eat fiber-rich foods, based on the work of Curry and colleagues. Curry and colleagues developed an algorithm and set of questions to determine at which stage individuals were for dietary fat reduction (Curry et al., 1992)(Table 8). Their results suggested that individuals could be clearly classified into one of five stages of dietary fat reduction, which were inversely associated with self-reported intake of fat (-0.93 for men, 073 for women in Sample B, p<0.001). Therefore, their algorithm was modified to readiness to consume adequate fiber, for the purpose of stratifying subjects in this study. The subjects’ stage of change relative to fiber intake was determined by their responses according to the algorithm. Although there was no a priori reason for a particular time span for a particular dietary behavior (Spomy et al., 1995), and no study has addressed stage of change for fiber intake (Glanz et al., 1994), we chose six months to be the time span for maintaining high fiber diet. Six months was selected based on the remarks by Prochaska and colleagues, “Being able to consistently engage in a new incompatible behavior for more than six months are the criteria for considering someone to be in the maintenance stage” (Prochaska et al., 1992). The SC questionnaire was modified from multiple-choice questions into a flowchart (Appendix F) for several reasons. 1) The flowchart appeared to be easier and quicker for subjects to answer than a multiple choice format. 2) The format is shorter and clearer, thus subjects’ interest of answering should be enhanced. 3) The original questions and algorithm by Curry et al. left some people between stages; the flowchart format avoids this problem entirely. By using the flowchart-style Stages of Change questionnaire adapted from Curry’s algorithm (Curry et al., 1992), each subject can be categorized into one stage of change related to his/her attitude and consumption of dietary fiber. 41 Table 8 Swing questions and algorithm for dietary fat reduction used by Curry et al.,1992 Questions 1. Have you ever changed your eating habits to decrease the amount of fat in your diet? Yes 1; No 2 (Skip to #2) 1A. If Yes, are you currently limiting the amount of fat in your diet? Yes 1; No 2 (Skip to #2) 181. If Yes, how long have you been limiting the amount of fat in your diet? Less than 30 days 1; 1-6 months 2; 7-12 months 3; Over 1 year 4 182. If Yes, would you say your are now eating a low-fat diet? Yes 1; No 2 2. In the past month, have you though about changes you could make to decrease the amount of fat in your diet? Yes 1; No 2 2A. How confident are you that you will make some of these change during the next month“? Very confident 1; Somewhat confident 2; Mildly confident 3; Not at all confident 4 Algorithm Stage Question(s) Answer(s) Precontemplation l or 1A No 2 No Contemplation 1 or lA No 2 Yes 2A Mildly or not at all confident Decision/Preparation l or 1A No 2 Yes 2A Somewhat or very confident Action I and 1A Yes 18 6 months or less Maintenance 1 and 1A Yes 13 7 months or more 42 Attfiydes and Beliefs Questionnaire The Attitudes and Beliefs (AB) questionnaire was designed for assessing subjects’ attitudes and beliefs related to dietary fiber intake. Carruth and Anderson proposed that scaling criteria for developing and evaluating an attitude instrument should include determination of content validity, test-retest reliability (stability), and internal consistency reliability (unidimensionality) (Carruth et al., 1977). In this cross-sectional research, only content validity and internal consistency reliability were examined. Glanz and colleagues defined three domains of psychosocial factors as sensitive indicators of dietary behavior improvement which are : predisposing, enabling, and change-related factors (Glanz et al., 1993). A 12-construct, 24-item questionnaire based on these three domains was developed and tested on the 652 participants of worksite health promotion interventiOn. Results showed that self-rated diet, past success at change, and motivation to eat low-fat foods were factors most strongly associated with dietary intake. Adopting these three constructs and building on the work of Glanz and colleagues, we designed our questions to assess subjects’ dietary attitudes and beliefs related to fiber consumption. . Other researchers have used the Health Belief Model (Rosenstock, 1974) for constructs related to attitudes and beliefs important for dietary behaviors. Contento and Murphy found dietary self-changers were differentiated from non-changers primarily on the basis of perceived susceptibility to diet-related diseases, perceived benefits of changing their diets, normative beliefs, overall health concern, cues to action, chance locus of control, and self-efficacy (Contento et al., 1990). Likewise, Schwarzer proposed that self- efficacy was a major determinant of behavioral intention in both the decision-making phase and the action phase (Schwarzer, 1992). Therefore, questions related to these factors were added to the AB questionnaire in order to increase its content validity and to relate responses to appropriate Stages of Change. Several questions on the AB questionnaire were adapted for fiber from the original questionnaire developed by Glanz and colleagues (Glanz et al., 1993). Most questions were 43 designed according to appropriate constructs of the HBM (Rosenstock, l974) to reflect stages of readiness to consume foods high in fiber. At first the questions were grouped into four constructs for the pilot study: I) predisposing factors; 2) enabling factors; 3) change- related factors; and 4) self-efficacy, but later the titles of these constructs were removed for concrseness. Expert reviewers determined the soundness of the questions for assessrng the fiber-related attitudes and beliefs to assure the content validity. For conciseness, items With less face validity and complex wording were eliminated. The final edition of the AB questionnaire (Appendix G) contained 13 questions, each question With a numeric Likert response scale answers ranging from 1 = strongly disagree to 5 = strongly agree. Statistical analyses The collected data were analyzed with the use of MSU NutriGuide 2.0 (Song W.O., Nutritional Analysis Computer Program, Michigan State University, 1990), SPSS 6.1 for Windows (SPSS Inc, Chicago, IL, 1995) and Excel 7.0 (Microsoft lnc., Seattle, WA, 1995). The descriptive statistics of subjects’ demographic characteristics including age, sex, height, weight, and BM were analyzed. From the checklist, each subject’s gram amount of fiber intake was determined from the fiber content of each food in a standard portion multiplied the frequency of consumption, then summed for all foods selected. From the 2- day food record, the average daily intake of fiber was the sum of 5/7’s of the weekday intake plus 2/7’5 of the weekend intake. Different weights were applied to reflect subjects’ different eating pattern during the weekday and weekend. The size, mean, and standard deviation of fiber intake assessed from checklists and weighted 2-day food records of each subgroup were calculated. Validity of the checklist was examined by three methods: 1) Pearson’s correlations of gram amount of fiber intake between checklist and weighted 2-day food records; 2) paired t-tests conducted on the gram amount of fiber intake assessed by the checklist and 44 weighted Z-day food records; and 3) the extent of sensitivity of the checklist to predict those at risk for low intakes of fiber. Low intake of fiber was defined two ways: 1) as _<_ 10 g/day based on findings by Smallwood and Blaylock using data from CSFII (Smallwood et al., 1994) where people not aware of health problems consumed less than this amount; and 2) as less than adequate or 20 g/day. High intake of fiber was 2 20 g/day because NCI recommends 20-30 g fiber intake per day. The definition of sensitivity in this study is the percentage of subjects whose actual fiber intake is defined as low (_<_ 20 g/day) confirmed by checklist (Greenberg et al., 1993). Subjects’ responses to SC questionnaire were coded 1 through 5, corresponding to precontemplation through maintenance, for statistical analysis. In each stage, the distribution of subjects and average daily intake of fiber assessed from the checklist and weighted 2-day food record were calculated. ANOVA (Duncan’s multiple range test) was used to test the significant difference of fiber intake among these subgroups. The distribution of subjects in each stage combining with the results of exploratory factor analysis from AB questionnaire were used to explain the characteristics of attitudes and beliefs related to fiber intake. The mode and mean (i SD) of each question on AB questionnaire for each stage was determined to reveal particular characteristics of attitudes and beliefs related to fiber consumption. Exploratory factor analysis was performed to determine how many constructs really existed in the AB questionnaire and then appropriate construct titles were given. The internal consistency of each construct was examined and the specific attitudes and beliefs at each stage of change were determined by subject’s response to each construct. Responses to AB questionnaire were cross-tabulated by distribution of Stages of Change to see whether responses made sense conceptually according to stage of readiness to consume fiber-rich foods. In additional analyses, the rank of foods from the checklist was calculated based on the contribution to the weekly number of portions and grams of fiber. The qualitative 45 responses to the barriers and suggestions to increase fiber intake were tabulated. Results and implications of this research will be provided to the Healthy U Program to aid in future evaluation and program design. Schedule Spring/95 *Started proposal writing Summer/95 *Finished draft questionnaire and checklist *Had 2nd committee meeting *Acquired UCHRIS approval Fall/95 *Examined 3-day food records from previous Healthy U results *Designed the format of checklist and questionnaires Spring/96 *Finished preliminary data analysis of foods selection for the checklist *Finished questions wording for the AB questionnaire *Had 3rd committee meeting *Made the checklist and questionnaires ready Summer/96 *Started literature review *Completed pilot test on secretaries of FSHN Dept. Fall/96 *Obtained the permission from MSU CTU *Mailed out the test instruments and started data collection from MSU CTUs. *Had 4th committee meeting *Started thesis and manuscript writing Spring/97 *Submit manuscript to the Journal of American Dietetic Association *Finish all research works 46 Results Of the 401 subjects contacted, 74 (18%) returned refusals to participate. In total, 99 (25%) subjects responded: 96 completed food frequency checklists; 67 completed 2—day food records; and, most of the subjects were female (90%). The gender distribution was similar between subjects who responded (n=99) and total subjects contacted (n=401). There appeared to be a slightly higher percentage of men than women who refused to participate. See Table 9 for the distribution of respondents by gender. The mean (i SD) age for all respondents was 42.8: 17.1 years. See Table 10 for detailed demographic statistics. The demographics for gender and age of the respondents was identical to those of the total CTU pool (n=1891). Table 9 Response rate and demggraphics of subjects Male Female All Total subjects contacted: 36 365 401 number of responders: 9 90 99 number of returned refusal: 10 64 74 number of non-respondents: 17 214 231 Subjects completing Checklists: 8 88 96 Subjects completing Zday Food Records: 7 60 67 Subjects completing both instruments 7 57 64 47 Table 10 Mean (: SD) of age, height, weight, and BMI of subjects gy gender Male (n=7) Female (n=60) All (n=67) Mean Age: (yr) 42.1 :11.8 41.3: 10.3 42.8: 17.1 Mean Height: (in) 71.3: 6.4 65.5:32 66.1 :40 Mean Weight: (lb) 184.1 :45.2 147.2: 27.6 151.0:316 Mean BMl: (kg/m2) 25.3:3.9 24.1 :37 24.2: 3.7 Figure 6 shows the scatterplot of fiber intake data from the two dietary assessment tools. Figure 6a, 6c show the distribution of the original fiber intake data collected from checklists and weighted 2-day food records, respectively. Distributions were skewed and needed to be normalized for correlational analyses. Figures 6b, 6d show the logarithmic distribution of the original data. All following statistical analyses were performed without the subject who reported 95 g/day of fiber intake (Figure 6a), a physiologically impossible intake, because this abnormal value would be a cause of significant bias. 8 50 (D a. o 'O 8 40- I..— 'C.’ N O 30‘ ° 0 . E . ‘ g . . o . 00 . . g 20‘ .0 .09. .0. : . to: . ‘ o . . t 0.. o . H— o o g 10l o .0 :0 9‘. . t. 93 U) 0 t v v V O 10 20 30 40 50 gram of fiber assessed from checklist Figure 6: Distribution of daily fiber intake from checklist vs. weighted 2oay food records 48 30 Figure 6a. Original distribution of fiber intake (9) from checklist (n=96) Figure 6b. Logarithmic distribution of fiber intake (9) from checklist (n=96) 49 2%.: 1.5 .5 .5 .5 . ' 37'.5 42.5 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 Figure 6c. Original distribution of fiber intake (9) from weighted 2—day food records (n=67) .88 ' 1.00 ' 1.13 ' 1.25 ' 1.3a ' 1.50 ' 1.63 Figure 6d. Logarithmic distribution of fiber intake (9) from weighted 2-day food records (n=67) 50 Although the fiber intake data appeared to be normally distributed after log transformation, the log values were only used in the calculation of correlation coefficients because it is not appropriate to describe if the subject was at the risk of low fiber intake or not using log values. Hypothesis 1. There will be positive correlation between the gram amounts of dietary fiber intake assessed fiom the checklist andfrom the 2-day food records. The daily fiber intake assessed from checklists was 16.9: 12.5 g/day, ranging from 3 to an abnormally high intake of 95.5 g/day. After the subject with abnormal fiber intake was excluded, the daily intake became 16.1 :9.6 g/day, ranging from 3 to 47.6 g/day. The daily fiber intake assessed from weighted 2-day food records was 16.1 :7.3 g/day, ranging from 6.3 to 45.1 g/day. Both original and logarithmic data showed significant correlation between the checklist with the 1" day and the average of the weighted 2-day food records (Table 11), suggesting that the eating pattern of subjects might have a larger fluctuation on weekend days than that on weekdays. Although the fiber intake appeared higher on weekdays than on the weekend, there was no significant difference (p=0.12) The range of Pearson’s correlation coefficiences were lower (r=0.48 to 0.55, p<0.01) than those expected and desired (r=0.60 to 0.80). Table 11 Median and mean (: SD) of average daily fiber intakes form checklist and weighted 2-day food record and log transformed correlation Instrument N' Mediagg) Mean: SM Correlation (r) Checklist 95 13.6 16.1 :96 —- weighted 2-day food record: 66 Log 1" day (weekday) 15.0 17.0 : 8.5 545* 479* 2"" day (weekend) 14.0 15.4:8.6 .233 .198 averge 14.9 165:7.4 .519" .481‘ ' number of paired t-test performed: 64 "' p<.01 5| The range of “limits of agreement” , which indicated how the difference in grams of fiber intake between two measurements distributes, was calculated by following formula (Saba et al., 1995) : ' mean difference (0.33 9) : I. 96 X standard deviation of the differences (9.42) The large range of “limits of agreement” (-18. 12, 18.80) also confirmed the moderate correlation between these two measurements. Based on these results, the Hypothesis 1 was weakly supported. Hypothesis 2. There will be no significant diflerence between the gram amounts of dietary fiber assessed from the checklist and the 2-day food records. The paired t-value between the fiber intake from these two measurements was 0.29 (df=63, 95% Cl= -2.015 to 2.693). Because the 95% confidence interval includes 0 in this range, no significant difference was detected in gram amounts of dietary fiber assessed from both measurements. Thus, the Hypothesis 2 was supported. Hypothesis 3. The sensitivity ofthe checklist to detect high and low intake ofdietaryfibcr will be _. 70 when compared to 2—day/ood records. The distribution of subgroups for high, medium, and low fiber intake is shown in 52 Table 12. The number of subjects being classified in the same subgroups by the two dietary assessments is 32 (7+17+8), which divided by total valid subjects (n=66) is defined as the percentage of agreement (0.48) between the checklist and weighted 2-day food record. To calculate sensitivity, subjects in Table 12 with fiber intake < 20 g/day from both dietary assessments were grouped (n=40) as well as the subjects with fiber intake < 20 g/day from the weighted 2-day food record but 2 20 g/day from checklist (n=10). The sensitivity, or the ability to detect subjects who actually had low fiber intake, of the checklist was 0.80, is higher than the expected value (0.70). Therefore, the Hypothesis 3 was supported. Table 12 The distribution of subjects (n=66) in high, medium, and low subgroups of fiber intake assessed by checklist and wjghted 2-day food record weighted 2-day food record Low Medium High Checklist (<10 g/d) (10 to <20 gfl) (_>_20 g/d) Low (<10 g/d) 7 11 3 Medium (10 to <20 g/d) 5 17 5 High (320 gld) 1 9 8 Hypothesis 4. I here will be a significant difference between the gram amounts ofdietary fiber intake for different stages ofchange related to dietary fiber. To address this hypothesis, subjects were classified into stages of readiness to consume adequate dietary fiber as assessed by the SC questionnaire. Figure 7 shows the distribution of subjects by stage of change, with number of cases on the tips of the bars. The largest group was people at the Maintenance stage, claimed by 35% of all subjects. Eighteen subjects did not indicate their stages on the flowchart, a surprisingly large number. 53 4O 30‘ 20‘ 10' Count 0 Missing Contemplation Action ’ Precontemplation Preparation Maintenance Figure 7. Distribution of subjects in five stages of readiness to change fiber intake (n=99) (number of subjects are shown at the tip of bars) The results for mean (: SD) fiber intake at each stage assessed by both instruments are shown in Table 13. Duncan’s multiple range t-test revealed that the fiber intake assessed by the checklist at the Maintenance stage was significantly higher than that at the Precontemplation and Action stages. No significant differences were found between stages for the fiber intake assessed from weighted 2-day food records. Although people at the Preparation stage appeared to have highest amount of fiber intake from both measurements, the differences were not significant by either dietary assessment measure, perhaps due to the small number in this stage. 54 Table 13 Fiber (9) assessed from the checklists and weighted 24iay food records at each stage of change Stage Checklist“ wgghted 2-d Food Record‘I Precontemplation 12.5:90 (17)” 14.9:96 (14) Contemplation 14.7: 10.6 (11) 13.8: 3.9 (6) Preparation 20.4:138 (6) 18.4:7.1 (5) Action 13.1 :72 (13)* 15.8: 3.7 (7) Maintenance 19.8:9.3 (34) 17.0: 7.9 (23) All valid cases 165:9.9 (81) 16.1 :7.5 (55) ' Mean:SD (n) b Duncan’s multiple range test, Precontemplation, Action vs Maintenance * p<.05 Table 14 shows the Speannan’s rank order correlation between stage of change with estimated grams of fiber intake from checklist and weighted 2-day food record. We found a positive, significant correlation between the checklist and stage of change, not too surprisingly because both are based on people’s self-perception of their diet and attitudes. However, there was no correlation between stage of change and estimated fiber intake from weighted food records which reported foods actually consumed for 2 days (a weekday plus a weekend). Table 14 Spearman’s rank order correlation between stage of change and estimated grams fiber intake from two instruments Instniment N Spearman's r Checklist 81 367* weighted Zday food record 55 .176 ' p<.005 55 The Hypothesis 4 was supported in part for the checklist. The fiber intake at Precontemplation stage is the lowest and was the highest at Maintenance stage. However, a clear stepwise progression from low to high intake by stage of readiness to eat fiber-rich foods was not observed, although there was a low but significant correlation for the checklist. The low intake for the Action stage is puzzling. Hypothesis 5. Subjects" responses to the attitudes and beliefs questionnaire will difler according to their different stages of readiness for dietary fiber. Table 15 shows subjects’ responses to the 13 questions on attitudes and beliefs related to fiber intake and the original constructs. In general, subjects knew about the health benefits of fiber (Q1 , Q2, Q9, Q13) and showed interest on fiber-rich foods (Q3, Q10, Q12). Subjects’ responses were neutral to environmental factors (Q8, Q1 1) and childhood history of high fiber intake (Q4). However, subjects reported overall a lack of social and family support for eating fiber-rich foods (Q5, Q6, Q7). 56 Table 15 Mode“, mean, and standard deviation of subjects’ (n=95) responses to Attitudes and Beliefs guestions Mode Mean SD Q1 Eating a lot of fiber-rich foods decreases my chances of getting serious diseases such as cancer and heart disease. 4 4'0 0'94 Q2 What I eat is important for my health 5 4.6 0.74 Q3 Fiber-rich foods taste good 4 3.9 0.96 Q4 I ate a lot of fiber-rich foods when I was growing up. 3 3.1 1.08 QS I've gotten a lot of advice about how to eat fiber-rich foods. 1 2.9 1.18 06 My friends encourage me to eat fiber-rich foods 1 2.0 1.06 Q7 My family encourages me to eat fiber-rich foods. 1 2.3 1.18 Q8 It is easy to select fiber-rich foods when I eat out 3 2.8 1.09 Q9 Eating fiber-rich foods is important 5 4.4 0.85 Q10 I‘m willing to try a new food if it is fiber-rich 4 3.7 1.05 Q11 It is hard to increase my intake of fiber-rich foods 3 3.0 1.10 012 I can increase intake of fiber-rich foods in next 6 months 4 3.8 0.88 013 I can be healthy, if I eat fiber-rich foods 4 4.0 0.98 ' Likert scale from 1=Strongly Disagree to 5=Strongly Agree When exploratory factor analysis was performed, the five constructs emerging from these questions were: Health benefits of fiber; Social and environmental support; Interest in fiber-rich foods; Childhood intake; Difficulty of increasing intake. These five constructs accounted for 73.1% of the total variance of responses. Table 16 shows the percentage of variance for which each construct accounts. 57 Table 16 The percent—ang variance of responses to A8 questionnaire for which each construct accounts Construct Pementagg Cumulative Percentage Health benefits of fiber 28.5 28.5 Social and environmental support 17.2 45.6 Interest in fiber-rich foods 10.7 56.3 Childhood intake 9.0 65.4 Difficulty of increasLngintake 7.7 73.1 Questions were put together which had correlation coefficients 2 0.60 for a specific construct. Table 17 shows the correlations between the 13 questions for the five constructs. Confinnatory factor analyses were performed for the questions with correlations < 0.60 and linked to more than one construct, e.g. Q7 and Q13, to decide to which constructs the questions belong (Hunter, 1977). As a result, the construct of “Health benefits of fiber” included Q1, Q2, Q3, Q9, and the less dominant Q13. Construct of “Social and environmental support” included Q5, Q6, Q7, Q8. Construct of “Interest in fiber-rich foods” included Q10 and Q12. Both the constructs of “Childhood intake”and of “Difficult of increasing intake” had only one question each which were Q4 and Q11, respectively. The a reliability of the three constructs with more than one question ranged from 0.61 to 0.83 as shown in Table 17. 58 Table 17 Correlations ' of each question with five constructs and or of three constructs with more than one gjiestion Health benefits Social and Interest in fiber- Childhood Difficulty of of fiber environmental rich foods intake increasing support intake Q1 .80 02 .86 Q3 .66 Q9 .84 013 .42 .52 05 .78 Q6 .81 Q7 .64 .42 08 .68 010 .72 Q12 .83 Q4 .93 01 1 .89 a .83 .73 .61 —- -- ' only correlations > .40 are shown Table 18 shows the responses to the 13 questions within the five new constructs by subjects at different stages of readiness to consume fiber-rich foods. For the construct of “Health benefits of fiber”, subjects across all five stages thought fiber in the diet was important to health. People at Preparation stage, however, did not seem to like the taste of fiber-rich foods (Q3). This finding was interesting given that subjects at the Preparation stage reported high intakes of fiber. These people were probably at the transition of adopting new foods and perhaps experiencing difficulty in the interim. Responses to the construct of “Social and environmental support” was low for all stages. People at Preparation and Maintenance stages appeared to be getting the most advice. The highest scores overall for “Social and Environmental support” were at the 59 Maintenance stage, as expected in SCT. Unfortunately average support from friends (Q6) and family (Q7) was still low or neutral, even at the Maintenance stage. Subjects at all stages above Precontemplation showed moderate self-efficacy (Q10, Q12) in the response to the construct of “Interest in fiber-rich foods”. There was no apparent relation between stages of change for fiber and the two one-question constructs—“Childhood intake of fiber rich foods” and “difficulty of increasing intake”. Because a one-question construct is inadequate to contribute to a scaled score—these two single question constructs were dropped from further analyses. Table 18 The mode“ of responses to Attitudes and Beliefs questions and their distribution by five stages of change Health benefits of Social and Interth in Childhood Difficulty of fiber environmental fiber-rich intake increasing intake support foods Stagefln) 01 02 03 09 013 05 Q6 07 08 010 012 04 011 P (17) 4 5 4 4 3 2 1 1 3 3 3 3 4 C(10) 4 5 4 5 4 1 1 1 3 4 4 2 2 D (6) 4 5 2 5 3 4 1 1 3 4 4 3 3 A (13) 4 5 4 5 5 3 1 2 3 4 4 3 4 M (35) 5 5 4 5 5 4 2 3 4 5 4 3 3 ' Likert scale from l= Strongly Disagree to 5 = Strongly Agree b P= Precontemplation, C= Contemplation, D= Preparation, A= Action, M= Maintenance To examine if subjects’ responses to first three constructs (with two to five questions each) would demonstrate significant difference by stage, the “score” of each construct was calculated. The score of a construct was defined as the sum of subjects’ response, based on the Likert scale, to all questions within the construct. Table 19 shows the mean (:SD) of the scores for each construct at different stages. Subjects at the Maintenance stage had the highest scores (p<.05) for the construct of “Health benefits of fiber” compared to those at 60 other four stages. The social and environmental support that precontemplators and contemplators perceived for consumption of fiber-rich foods was significantly less than what people at the Maintenance stage perceived. Precontemplators also had significantly less interest in fiber-rich foods than people at other stages, except for Preparation, confirming the concepts of SCT. These three constructs only accounted for 56.3% of total variance of responses, indicating they did not completely reveal all attitudes and beliefs related to fiber consumption. Thus, Hypothesis 5 was particially supported. Table 19 Mean (: SD) of the scores for three major constructs at the subjects’different stages of readiness to consume fiber Health benefits of Social and Interest in fiber-rich foods fiber (5) ‘ environmental support (2) (4) Precontemplation (17)b 19.9: 2.4” 8.3: 2.6"c 6.4: 2.0 Contemplation (10) 20.9: 2.6‘ 8.4:3.1* 77:11"d Preparation (6) 18.8: 2.8“ 92:29 7.8: 1.7 Action (13) 21 .2:2.6* 10.2:39 8.1 :1 .5" Maintenance (35) 22.8:2.1 11.3: 2.9 78:15" ' number of questions in the constnict b number of subjects at the stage .- Duncan’s multiple range test, stages vs Maintenance " Duncan‘s multiple range test, stages vs Precontemplation "' p<.05 Hypothesis 6. Subjects 'jiber intake from the 2-day food records can be predicted from 3 short instruments: the checklist, SC questionnaire, and the major constructs from the AB questionnaire Multiple regression (enter) was used to determine if fiber intake from the weighted 2- day food records (dependent variable) could be predicted from the fiber intake from 61 checklist, the subject’s stage of change, and scores of the three major constructs in AB quesitonnaire (independent variables). Table 20 shows the hierarchical and stepwise prediction of dependent variable from five independent variables. Table 20 Stepwise prediction of fiber intake from weighted 2-day food record using fiber intake from checklist, the subject’s stage of change, and subject’s scores of the three major constructs in AB guesitonnaire Step Variable r R2 p 1 Gram amounts of fiber intake from checklist .52 .27 <.001 2 Stages of Change .13 .02 .35 3 Construct of “Health benefits of fiber“ .17 .03 .20 4 Construct of “Social and environmental support” .25 .06 .06 5 Construct of “Interest in fiber-rich foods” .19 .03 .17 The regression equation was: Y = 0.46 X1 + 8.54 X I : gram amounts of fiber assessed from the checklist (n = 64; F = 29.43; df= l; p< .0001) Y .' gram amounts of fiber assessed from the weighted 2-day food record Other independent variables (step 2-5) are not shown in the equation because their correlations with the dependent variable are not significant, although the construct of “Soical and environmental support” showed a low correlation that approaches significance (p=0.06). The five independent variables predicted a total of 44% of the variance in fiber intake—suggesting that the relationship is not likely linear. Therefore, hypothesis 6 was not supported for this population of predominatly women. 62 Additional Analysis From the checklist, the rank of foods for their contribution to total fiber intake was calculated based on two factors: the weekly number of portions consumed; and the grams of fiber consumed per week by an individual. Table 21 shows the top 20 foods for each of these two factors. Wheat bread was the food with the largest weekly consumption and apple was the food providing largest amount of fiber in the subjects’ diet. Table 21 Ranking of foods from the checklist for their contribution to total fiber intake based on the average weekly number of portions consumed and grams of fiber consumed from that food per week by each subject Food no. of portion/wklperson Food grains of fiber/wklperson wheat bread 4.50 apple 8.32 apple 3.18 whole grain cereal 7.95 white bread 2.85 wheat bread 7.16 lettuce 2.74 bran cereals 7.05 tossed salad 2.57 potato 5.38 potato 2.56 banana 5.38 banana 2.48 prunes 5.01 tomato 2.28 refried beans 5.00 carrots 2.25 tomato 4.88 bagel 1 .96 oatmeal 4.37 whole grain cereal 1.91 peanut butter 4.36 pasta 1 .45 carrots 3.96 crackers 1.42 baked beans 3.65 broccoli 1.40 green peas 3.16 green beans 1.34 green beans 2.92 peanut butter 1.07 broccoli 2.83 com 1 .05 pasta 2.72 tortillas 0.99 bagel 2.45 oatmeal 0.96 orange 2.30 bran cereals 0.94 pear 2.08 popcorn 0.91 tossed salad 2.01 63 Shown in Table 22 are foods that subjects in this study ate frequently, but were not listed on the checklist. The foods selected more than three times (white rice, cabbage, pepper, grapefruit) might be necessary to be included in the checklist in the future to decrease the inaccuracy between the data from the checklist and actual intake. Table 22 Foods not listed on the checklist but were selected by subjects more than once Food Times being selected White rice Cabbage Pepper (green, red) Grapefruit Feud Lentil Pinto bean Kiwi fruit Black bean Sunflower Cucumber Almond Onion Gabanza bean . Sesame seed Bulgar Pecan Radish mmmmmmmmwwwwwwekam 64 Subjects’ responses were tabulated in Table 23 to the two open-ended questions “What could you do to increase your intake of dietary fiber ?” and “What keeps you from eating more fiber-rich foods ?” in the AB questionnaire. Generally, subjects thought the best way to increase intake of dietary fiber was to consume more fiber-rich cereal, fruits, and vegetables. It is noticable that two subjects reported use of fiber supplements was a way they would increase their fiber intake. The major factors for avoidance of fiber-rich foods were lack of convenience, not enough time to cook, difficulty in having fiber-rich foods on the go or when eating out. Concerns about the gas production and unpaltable taste of fiber-rich diet were also significant reasons for not eating fiber-rich foods. TabI023 Subjects’ response to suggestions for and barriers to increasing fiber intake (n=95) What could you do to increase your intake of dietary fiber ? Counts . Eat more fiber-rich cereals, fniits, and vegetables 40 Improve meal planning 7 Avoid empty calorie snacks 7 Increase availability of fiber-rich foods 5 Learn how to select fiber-rich foods 3 Use fiber supplements 2 What keep you fiom eating fiber-rich foods .7 Lack of convenience (time to prepare, availability) 30 Health concerns (on special diet, avoid gas production) 13 Lack of interest to change 11 Unpalatable taste of fiber-rich foods 8 Feelings of enough consumption 6 Influence from family or spouse 4 Cost 4 Strict dietary preference (small appetite) 3 Lack of knowledge for selecting fiber-rich foods 3 Lack of recipes 1 ‘ based on number of opinions generated from open-ended questions, not number of subjects 65 Discussion The high sensitivity (80%) of the checklist demonstrated its ability to distinguish subjects who actually ate low fiber diets from those who reportedly ate low fiber diet. High sensitivity of a dietary assessment instrument is required for intervention programs to ensure the strategies are targeted to those who actually are at dietary risk, even though they might regard their diets as adequate. The good sensitivity of the checklist supports its feasibility and effectiveness of use as a screening tool for a population similar to this sample at high risk for insufficient intake of dietary fiber. Checklist Validation The correlation between the test checklist and the criterion measure of weighted 2-day food records was 0.52, which was identical to the result of a similar study by Saba et a1 (1995). Low to moderate correlation (r =0.30-0.50) in measuring dietary fiber intake between the test instrument and criterion measurement seemed prevalent in the studies reviewed. The short checklist developed by Kristal et al. had a correlation of 0.40 with mean of two 4-day food records (Kristal et al., 1990). The FFQ developed by Willett et al. had a correlation of 0.46 with mean of four 7-day food records (Willett et al., 1985). The FFQ developed by Jain et al. had correlations of 0.39 for men and 0.52 for women with one 7-day food record (Jain et al., 1996). Moderate correlations between two dietary assessment techniques might be caused by random errors incurred in measurement of the diet by both methods. Sources of random errors include: 1) subjects forgetting to record what they ate; and 2) over- or underestimate of actual consumption. Other sources of error include: 1) checklist or FFQ design does not match subject’s ordinary food choices; 2) the foods not included in the database of the 66 analytical software were mistakenly substituted by researcher’s subjective interpretation; and 3) incorrect portion size transformation by researchers. All methods of dietary intake measurement require either a direct probe of portion size or referral to a standard size in order to estimate energy or nutrient content. If portion sizes are estimated incorrectly, this source of error itself might be sufficient to explain the discrepancy between self-reported caloric intakes and rates of obesity in the US. (Young et al., 1995) Moreover, studies have shown that in fact subjects pay little attention to portion size information on FFQ (Subar et al., 1995). The moderate correlation between the checklist and the weighted 2—day food record in this study might largely result from this reason. Another reason for the moderate correlation might be subjects’ confusion on filling out the checklist. It was quite common to find subjects entering self-contradictory responses (> 80%), e. g., filling in 1 time daily and 3 times a week for the same food. This problem was addressed by uniformly choosing the answer with largest frequency, which might have led the checklist to overestimate actual fiber intake. A third reason for the moderate correlation might be that foods were not on the checklist, but were selected by the subjects, forming a missing source of fiber intake. There were 36 subjects (36%) who indicated at least one food they frequently ate which was not shown on the checklist. A few of these foods such as green pepper and cabbage might be significant fiber sources. The fiber content of these foods was not included, because it would have contaminated the validation check of the checklist in this study. The ability of the checklist to classify subjects into low, medium and high intakes, or percentage of agreement with the weighted 2-day food record, was a modest 47%, but similar to that found in the self-administered FFQ (53%) where Saba and colleagues used a 7-day weighed food record for validation (Saba et al., 1995). In the present study, 4 subjects (6%) were placed into opposite subgroups of fiber intake. That is, their fiber intakes were determined as high by one measurement but low by the other one. This misplacement 67 percentage for the checklist was lower in the present study than the 12% misplacement error in the study by Saba et al. Stages of Change Questionnaire Surprisingly 18 (18%) subjects returned their SC questionnaires blank, while they completed the other instruments. The problem might be caused by subjects’ lack of attention to the self-administered questionnaire, but it is more likely a sign of the subjects’ unfamiliarity with the questionnaire in flowchart format. Although the SC questionnaire did achieve the goal of stratifying subjects effectively, because no subject chose two stages at the same time, this might also be part of the problem, if some people did not fall neatly into one of the five stages. The large number of subjects in the Maintenance stage confirmed that a voluntary, health-related survey is more likely to recruit respondents who are self-conscious about diet and health. Table 24 shows a close comparison of fiber intakes at different stages. from the present study with those from studies done by Spomy (Spomy et al., 1995), Glanz (Glanz et al., 1994). and Huang (Manuscript in process, 1997). In this study, fiber intake did not increase significantly, perhaps due to the small sample size, compared to the significant increase of fiber consumption found earlier at the Action stage in both Spomy’s and Glanz’s studies. In Huang’s study, the significant increase was not found until the Maintenance stage. As a way to examine the theoretical relevance of the SC questionnaire to intake of dietary fiber, the mean and standard deviation of grams fiber consume at each stage among the cited studies were compared. Except for the Maintenance stage in Spomy’s study and the Contemplation and Preparation stage in Glanz’s study, there was no difference in the fiber intake at each stage between this study and the other two cited. These findings indicate fair compatibility between the checklist and the SC questionnaire in demonstrating 68 the relationship between the actual fiber intake and different stages of readiness to increase fiber consumption. Table 24 Comparison of gram amount of dietary fiber intake (mean:SD) by stage of change between Shih’s checklist and data of Glanz et al., 1994; Spomy et al., 1995; and Huanget al., 1997 n Precont. Contemplation Preparation Action Maintenance Glanz etal.,1994I 17,039 12.6:0.1’ 13.0.0.1b 13.8:02“ 15.4:01d 19.6:02" Spomyetal.,1995 618 118:2.8a 118:2.9a N/A 13.7:36" 14.7:3.7° Huangeta|.,1997 128 N/A 15.4.1.4: 165:1.1" 16.2:09” 19.6:1.2° Shihetal.,1997' 81 12.5:90‘ 14.7:106 20.4:13.8 13.1:7.2‘ 19.8:9.3 I Based on 2,000 kcal diet .4.- Means not sharing the same superscript are significantly different at p<.05 or beyond, using post-hoe Schefi’e constract. f Duncan’s multiple range test, Precontemplation, Action vs Maintenance * p<.05 Distribution of subjects across the stages of change Table 25 shows the percentage of distribution of subjects at each stage in this study compared with results from other studies. The highest percentage was at the Maintenance stage, indicating almost half of the subjects in this study were aware of benefits of dietary fiber and willing to maintain a fiber-rich diet. In Huang’s study, subjects were the participants of MSU Healthy U program who already seemed motivated to learn about a healthy lifestyle. The distribution by stages of change confirmed that assumption and revealed that the largest group was people at the Action stage. It is believed that a 69 voluntary, health-related mail survey is more likely to recruit respondents who are self- conscious about diet and health. Both the distributions of this study and Spomy’s confirmed that assumption, because the methods used for data collection were similar. It appears that when the survey is conducted on participants of intervention programs, however, the percentage of people at the Action stage tends to be higher than that at the other stages. Table 25 Distribution of subjects in five stages of change for fiber intakefrom several relevant studies n Precont. Contemplation Preparation Action Maintenance Shih et al., 1997 99 17.1% 10.1% 6% 13.1% 35.3% Huang et al., 1997 128 0% 7.3% 22.6% 54.7% 15.3% Spomy et al., 1995 618 7.7% 9.2% N/A 37.8% 45.1% Glanz et al., 1994 16,980 12.2% 28.1% 8.7% 33.4% 17.6% Attitudes and Beliefs Questionnaire The AB questionnaire showed a fair content validity with the three major constructs within in it accounting for 53% of the variance of subjects’ response to attitudes toward fiber consumption. The AB questionnaire demonstrated ability to reveal characteristics related to fiber intake at some stages of change. The major three constructs in questionnaire—health benefits of fiber, social and environmental support, and interest in fiber-rich foods— resemble three of 10 social psychological variables in Spomy’s study— perceived benefits, social modeling, and overall health concern (Spomy et al., 1995). 70 Subjects at the Maintenance stage in both studies reportedly perceived more health benefits of fiber and more social and environmental support to consume fiber-rich foods than their cohorts at the Precontemplation and Contemplation stage. Subjects at the Precontemplation stage were also found to have the lowest score in the construct of “interested in fiber-rich foods” in both studies. The rank of foods based on the quantitative contribution to total fiber intake Compared to Huang’s similar analysis of dietary fiber from 3-day food record of MSU Healthy U participants (n=128)(Huang, manuscript in process), subjects in this research showed similar dietary patterns for fiber consumption. Thirteen out of 20 foods appear in Huang’s list with slight difference in the order. Some less-frequently consumed foods, such as prune and refiied beans, were listed in the top 20 foods because of their high fiber content. Compared to Block and Lanza’s list of dietary fiber sources in U.S.(Block et al., 1987), analyzed from NHANES II data, a different dietary pattern of fiber consumption was found in the present study. The top 1 and 2 foods on Block and Lanza’s list were white bread and pinto and other dried beans, respectively, which were not in ranking of top 20 foods consumed by this population. White bread provided 11.4% of total fiber intake in NHANES II (n=1 1,658), but it only accounted for 1.2% in the present study. The top 1 food in the present study was apple, which accounted for 7% of total fiber intake but only 3.6% in NHANES II. 71 Strengths This research has the following strengths. 1) A high sensitivity (80%) for the checklist distinguished subjects who actually had low fiber intake from those who reportedly had high intakes. 2) The short administration time of the three test instruments was good. It took less than 10 minutes to complete the checklist and both SC and AB questionnaires, based on the responses from the pilot test. 3) The study prombted awareness among respondents of dietary fiber intake, a topic ofien neglected in health promotion interventions. 4) Good compatibility was found among three instruments to form a validated screening package for fiber intake and readiness to increase fiber consumption in this sample. Limitations This research also has the following limitations. 1) There was only a moderate correlation (0.52) between the checklist and the weighted 2-day food records for fiber intake. 2) There was a small sample size, due to the poor response rate of 25%. The large proportion of respondents not answering the SC questionnaire lowered the statistical power of analysis. 3) The SC questionnaire did not go through a separate validation process. 4) The 18% of non-respondents to the SC flowchart was worrisome and must be explored further before this tool can be recommended for use. 72 5) The two constructs of “Childhood intake of fiber” and “ Difficulty of increasing in take of fiber” had only one question each in the factor analysis, yet together accounted for nearly 20% of the variance in response to attitudes and beliefs. Future research might expand the number of questions for these two constructs so that they could be added to the scale of “Attitudes and Beliefs about Fiber”. The following reasons might be responsible for the low response rate in this study. 1) 2) 3) 4) 5) We were not able to send an official endorsement letter from the President of the CTU with the survey. Incentives were not attractive enough to the target population. The time-consuming 2-day food recording task reduced subject’s interest. Non-respondents might have had a negative influence on those who were hesitant at first and who worked within the same department, because subjects from the same department tended to be respondents or non-respondents collectively. Subjects had low interest in the health benefits of dietary fiber. 73 Conclusion This study demonstrated the development and validation of a new dietary assessment tool using behavioral approach. Results demonstrated that the combination of the theoretical model and brief dietary assessment was a feasible way to identify a high risk population before the intervention begins. 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Portion sizes in dietary assessment: Issues and policy implications. Nutr Rev. 1995;53:149-158. Appendices Appendix A 87 MICHIGAN STATE u N1 v E a s r r it September 10. 199‘ ro: Sharon Hoerr 204 on. Trout Building Michigan scaca University RE: rear: 95-411 rrrca: rue APPLICATION or srAcas or CHANGE rxaoar ON turnovanasr or socuaana AND INSOLUABLE orarAnr crass rsrAxa aavrsrou aaouasrso: 08/21/96 CAracoav: -c APPROVAL DATE: 09/18/96 The university Commiceaa on Raaaarch Involving Human Subjects'IUCRIKSl raviaw of this project is complete. I am ploaaad co advxsa than the rights and waltara of rho humnn gubjacca appear to be adequately pgocaccad and methods co obtain informad consanc are appropriate. b:ratora. cha UCRIHS approved this project and any ravxsions llscad a va. -. RIflIflhL: UCRIHS approval is valid tor one calendar year. beginning with the approval data shown above. Investigators planning co continua a projac: beyond one year must use the groan renewal torn (enclosed with c a original a proval lancer or when a projecc is renawadl to soak u dac certification. There is a mnxxmum of tour such axpadl ranawals possible. Investigators wishing to continua a reject beyond than time naad co submae I: again or complete rav aw; RIVISIONS: UCRIHS muse ravlaw any changaa in rocaduraa involving human subjects. prior to lnaciacion o! c a change. If this is done a: cha.clma o ranawal. plaasa uaa cba green renewal torn. To ravasa an approvad protocol a: an ochar time during the year, send your written request to the IRS Chair, requesting ravrsed approval and ratarancang cha project's IRS 3 and cicla. Include in your request a daacrapcron o: cha.change and any revised Instruments. consent forms or advarcrsamancs ehac ara applxcabla. PROBLIKSI . cnaxcrs: Should aichar of tho followin ariaa during the course of the work, investigators must not; DCRIHS promptly: (ll roblams (unexpected aida attaccs, comp azncs. ccc.) involving uman subjeccs or 12) changes 1n the research environmen: or new intermacron rndicaclng graacar rlsk co the human sub aces than exrscad when the prococol was pravzoualy reviewed an approved. I: we can be of any fucura help. lease do not hesitate to contact us an (517)355-2l80 or rAx (Sl7ls 2- I71. kl“ Sincerely. vid 3. Wright. CRIHS Chair DEH:b¢d Appendix B September 28, 1996 Dear MSU CTUs: The importance of dietary fiber to prevent several chronic diseases—including colonic cancer, diverticular disease, diabetes, etc—has been mentioned in various publications. However, the US. public seems less concerned about fiber than about fat in their diets. To promote the awareness of its health benefits by increasing daily fiber intake, we are going to conduct a survey about your readiness to change fiber consumption. Some of you will receive a package of short questionnaires via campus mail next week. We also will enclose a $1 coupon for MSU dairy store in the package and ofi‘er a $25 value of dietary assessment to express our appreciation for your participation. This survey has been granted by MSU Healthy U Committee and received the permission from the President of MSU CTU. Best Regards Sharon Hoerr, RD., PhD. ' Won Song, RD., PhD. Associate Professor Associate Professor Dept. of Food Science and Human Nutrition(FSPH\l) Dept. of FSHN Ralph Levine, PhD. PrOfessor Dept. of Psychology Appendix C 89 The purpose of this study is to develop aid validate: l) a short checklist to assess intake of dietary fiber. 2) a flowchart to determine where you at mlative to dietary fiber intake; 3)a questionnaire to assess your attitudes and behaviors related to dietary fiber. and 4) your ideas about how to increase the intake of fiber-rich foods. it will take you about 10-15 minutes to complete all three instruments. You are or course tree to refuse to answer any question that makes you uncomfortable. We are also asking you to provide a 2 IIIIII’ doing it? I No I l ' ' I I I l : yrs I YES : a I I I I V V ' I E . How confident are you that you I GOV” long have you done "7 > will start this change during the I No next month? I I I I I I I I I Very or . I somewhat M‘ldly 0‘ not at V More than 6 m°mh5 < Less than 6 months) confide,“ all confident Dept Food Scrence a: Human Nutrition Michigan State Universrty. Iwn Appendix G 93 What do you think about fiber-rich foods ? Aga‘n, fiber-rich foods mean Iruits. vegetables. nuts. beans. whole grain breads. and high-fiber cereah Stroneg Disagree Stroneg Agree I. Eating a let of fiber-rich foods decreases my chances of l 2 3 4 5 getting serious diseases such as cancer and heart disease. 2.‘What I eat is important for my health. I 2 3 4 S 3. Fiber-rich foods taste good. I 2 3 4 S 4. I ate a lot of fiber-rich foods when I was growing up. I .. 3 4 5 5. I've gotten a lot of advice about how to eat fiber-rich foods. I 2 3 4 5 6. My friends encourage me to eat fiber-rich foods. 1 2 3 4 S 7. My family encourages me to eat fiber-rich foods. I .. 3 4 5 8. It is easy to select fiber-rich foods when I eat out. I 2 3 4 S 9. Eating fiber-rich foods is important. I 2 3 4 5 10. I‘m willing to try a new food if it is fiber-rich. I 7 3 4 5 l I. It is hard to increase my intake of fiber-rich foods. I 2 3 4 5 I2. I can increase intake of fiber-rich foods in next 6 months. I 2 3 4 5 13. I can be healthy, if I eat fiber-rich foods. I 2 3 4 5 Your feedback to following questions will help Healthy U improve its program. I What could you do to increase your intake of dietary fiber? 2. What keeps you from eating more fiber-rich foods? Wires you are done with this pate. please told. staple and put It ln campus mall. tiles please complete the two day: toorl records. Dept I-u-rd Science k Human Nutrition Michigan State Uruversm. I'M. "illllllllllllllllllls