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This is to certify that the
thesis entitled

SCREENING EARLY MATURING SOYBEAN (Glycine max L.)
GERMPLASMFOR RESISTANCE TO THE SOYBEAN APHID
(Aphis glycines MATSUMURA)

presented by

Clarice Mensah

has been accepted towards fulfillment
of the requirements for the

MS. degree in Plant Breeding and Genetics
Program

 

 

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SCREENING EARLY MATURING SOYBEAN (Glycine max L.) GERMPLASM
FOR RESISTANCE TO THE SOYBEAN APHID (Aphis glycines MATSUMURA)

By

Clarice Mensah

A THESIS
Submitted to
Michigan State University
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
Plant Breeding and Genetics Program

Department of Crop and Soil Sciences

2004

ABSTRACT

SCREENING EARLY MATURING SOYBEAN (Glycine max L.) GERMPLASM
FOR RESISTANCE TO THE SOYBEAN APHID (Aphis glycines MATSUMURA)

By
Clarice Mensah

Since 2000 the soybean aphid (Aphis glycines Matsumura) has become a major
pest of soybean [Glycine max (L.) Merr.] in the USA. Currently none of the commercial
soybean varieties show resistance to the aphid and there are no sources of resistance
reported in early maturing soybean germplasm in the USA. The objective of this study
was to identify sources of resistance to soybean aphid from early maturing soybean
germplasm and to determine the type of resistance. Over a two year period, 2,147 early
maturing soybean accessions from maturity group (MG) 0 to 111, originally from northern
China, were screened for aphid resistance in the greenhouse and in field cages. The
plants were hand-inoculated and aphid populations were evaluated 10 days after
inoculation. A damage index (0-100%) was calculated for each accession. After two
years of screening and confirmation in choice tests, four accessions, P1567543C,
P1567597C, PIS67541B, and P1567598B, in MG 111 were found to be resistant to the
soybean aphid. Two of these accessions, P15675413 and P1567598B, possessed
antibiosis resistance preventing the aphids from reproducing on the plants in a no-choice
study. These resistant sources can be used to develop commercial varieties with aphid
resistance for the North Central states where soybean varieties of MG 0 to 111 are

cultivated.

DEDICATION
To my husband Doe and my little girl Volta Elolo Adovor. And also to the

Mensah Family in Kumasi — Ghana

iii

ACKNOWLEDGEMENTS

I would like to gratefully acknowledge the guidance of my major advisor Dr.
Dechun Wang and the members of my guidance committee, Dr. Chris DiFonzo and Dr.
Dave Douches. I also appreciate the immense help given to me by my coworkers in the
Soybean Breeding Program and the Field Crops Entomology Laboratory in the
inoculation and data collection aspects of this research. Many thanks goes to Dr. Randall
Nelson curator of the USDA Soybean Gennplasm Collection in Urbana, Illinois for
making the germplasm we screened readily available. Finally I wish to thank Project
GREEEN (Generating Research and Extension to meet Economic and Environmental
Needs) and the Michigan Soybean Promotion Committee for providing the funds which

supported this research.

iv

TABLE OF CONTENTS

 

 

 

 

 

 

LIST OF TABLES ' VI
LIST OF FIGURES VII
INTRODUCTION AND REVIEW OF LITERATURE 1
MATERIALS AND METHODS ‘ 12
RESULTS 18
DISCUSSION 26
REFERENCES 29

 

 

APPENDIX ’ 32

LIST OF TABLES

TABLE 1: NUMBER OF ACCESSIONS IN EACH DI CATEGORY FOR 2002 (N
= 1043) AND 2003 (N= 1108). 22

 

TABLE 2: RESULTS OF THE NO-CHOICE TEST FOR THE SIX RESISTANT
ACCESSIONS, RESISTANT AND SUSCEPTIBLE CHECKS, AND VARIETIES
IN 2003. 23

 

TABLE 3: DAMAGE INDEX (DI) BASED ON THREE REPLICATIONS IN 2004
FOR SIX PUTATIVE RESISTANT ACCESSION S, IDENTIFIED AFTER TWO
YEARS OF SCREENING, AND A SUSCEPTIBLE CHECK THREE AND FOUR
WEEKS AFTER INOCULATION. 25

 

vi

LIST OF FIGURES

FIGURE 1: ILLUSTRATION OF THE VISUAL RATING SCALE USED TO
ESTABLISH THE DAMAGING INDEX (D1). 17

 

FIGURE 2: DAMAGE INDEX CATEGORIES FOR CHOICE TEST IN 2002
CONDUCTED IN THE FIELD IN SUMMER AND IN A GREENHOUSE IN
WINTER AT MICHIGAN STATE UNIVERSITY 19

 

FIGURE 3: DAMAGE INDEX CATEGORIES FOR CHOICE TEST IN 2003
CONDUCTED IN THE FIELD IN SUMMER IN TWO SIMULTANEOUS
REPLICATIONS AT MICHIGAN STATE UNIVERSITY 21

 

vii

INTRODUCTION AND LITERATURE REVIEW

Soybean is the leading oilseed crop produced and consumed worldwide
(Hymowitz 2004). In the past half century the United States of America (USA) has been
the world’s leading producer. In 2003 alone, the USA produced 35% (65.8 million Mt) of
the world’s total soybean (FAOSTAT 2004). Soybean has many insect pests limiting its
production, including the soybean aphid. A native to eastern Asia, the soybean aphid was
not reported in the USA prior to July 2000. The consensus among soybean researchers is
that the soybean aphid was present but remained undetected for several years before
2000 (Ragsdale et al. 2004). Since then, the insect has rapidly spread to the major
soybean production areas in the USA and Canada (Plant Health Initiative, 2004). In 2000,
the aphid was found in Michigan, Indiana, Illinois, Minnesota, Wisconsin, Iowa, and
Missouri. In the following two years, the aphid spread to other states including Ohio,
New York, Pennsylvania, Delaware, Virginia, West Virginia, Kentucky, Kansas,
Nebraska, South Dakota, and North Dakota. In 2003, severe aphid infestation was
reported in many of these states. Outbreaks have been severe in the northern part of the
Midwest and in Ontario, particularly in 2001 and 2003.

In the USA, soybean aphid research is still in its early stages. The aphid was first
found in the USA in 2000 and annual infestations have been unpredictable (Steffey and
Gray 2004). The only available method of controlling soybean aphids in the USA is to
spray insecticides. Although the use of insecticides can be a quick and easy way to
control aphids, the ideal time to spray has not yet been well defined. Insecticides also

have many adverse effects, such as killing the natural enemies of aphids, and raises

chemical use and soybean production costs (Gao 1991). Aphid populations may resurge
when applications of insecticides are poorly timed. Developing soybean varieties that are
resistant to the aphid is a long-term solution to the problem.

To develop aphid resistant varieties, sources of resistance must be identified.
Sources of resistance to the soybean aphid are reported in China. In the late 1980’s, two
highly resistant varieties were found among 181 varieties screened (Fan 1988); in 1991,
resistance was also reported in Chinese soybean germplasm (Sun et al. 1991). The type of
resistance, antixenosis (non-preference) or antibiosis, was not indicated in these studies.
Antixenosis is nonpreference of insects for a host plant (Korgan and Ortrnan 1978).
Antibiosis includes all adverse effects on an insect’s life history afier a resistant host
plant has been used for food (Painter 1951). Knowing the type of resistance is important
to fully understand and utilize resistant accessions in a breeding program. Hill et a1.
(2004) have recently reported 3 lines with resistance to soybean aphid. PI 71502 (MG IV)
has antixenosis and the cultivars Dowling (MG VIII) and Jackson (MG VII) were
reported to have antibiosis resistance.

At the time our research was initiated in 2002, there were no known sources of
host plant resistance in the USA. The USDA Soybean Germplasm Collection at Urbana,
Illinois contains over 6,278 accessions of Chinese origin (E. Peregrine personal
communication), and we assume that some resistant accessions exist in this collection.
Therefore, we screened soybean germplasm collected from China to identify sources of
resistance from this collection.

The objectives of this study were to: (i) screen soybean germplasm from northern

China in the USDA Germplasm Collection to identify sources of resistance to soybean

aphids in early maturity groups; and (ii) determine the resistance type of the identified
sources.
THE SOYBEAN

Soybean, Glycine max (L.) Merr., (2n=2x=40) combines in one crop both the
dominant world supply of edible vegetable oil, and the dominant supply of high-protein
feed supplements for livestock. Other fi'actions and derivatives of the seed have
substantial economic importance in a wide range of industrial, food, pharmaceutical, and
agricultural products (Johnson 1987). The USA is the principal world supplier of
soybeans followed by Brazil. The USA, Brazil, Argentina, and China account for over 90
percent of world soybean production. (Soy Stats 2004). AS a source of protein, soybean is
often less expensive on a cost per kilogram basis compared to animal protein (Hymowitz
and Newell 1981)

Soybean is a papilionoid legume belonging to family Fabaceae. The genus
Glycine is divided into two questionably distinct subgenera: Glycine and Soja. The first
consists of six or seven perennial species primarily from Australia. The second consists
of three annual Species from Asia: Glycine max, Glycine soja, and Glycine gracilis. The
first species is the cultivated soybean, the second is the wild form of the soybean, and the
third the weedy form of the soybean (Palmer et al., 1996).

THE SOYBEAN APHID

The soybean aphid (Aphis glycines Matsumura) belongs to the Family Aphididae
and is a small, light yellow or yellowish-green insect with two distinct black cornicles
and pale tail (cauda). Its body color, black cornicles, and colonization of soybean (and

buckthom) distinguish it from other aphid species (V oegtlin et al. 2004). Adults of the

soybean aphid may or may not have wings. Adult soybean aphids are about 1/ 16th of an
inch (2 mm) long. The young look much like miniature Wingless versions of the adults.
Winged aphids have a black head and thorax.

Life History

The soybean aphid displays a complex life cycle (heteroecious and holocyclic)
with alternation of sexual and asexual generations and host plants (Ragsdale et al. 2004).
It utilizes two very different types of host plants in the USA, the buckthom (Rhamnus
spp) and soybean plants. The observed life history of soybean aphid in North America is
similar to that observed in China and Japan, with the exception of the primary (over-
wintering) hosts. In China and Japan, the most common over wintering hosts are
Rhamnus davurica Pallus and Rhamnusjaponica Maxim (Takahashi et al., 1993). In
North America, various buckthom (Rhamnus) species are used as primary hosts (V oegtlin
et al., 2004), particularly common buckthom (Rhamnus cathartica).

The soybean aphid over winters as an egg on the buckthom. In spring, the
Wingless mothers hatch fiorn the egg and produce colonies of Wingless females. These in
turn produce a third generation of aphids that includes winged emigrants. The winged
females fly to soybeans, the summer host for the aphid. For the remainder of the summer,
colonies of Wingless and winged females are produced. All asexual generations are
entirely female and are clones of the mother. Numbers of aphid generations range from
10 to 22 per year in China (Li et al. 2000). Wu et al. (2004) reported a total of 18
generations per year in China, with 15 of these generations occurring on soybean. In the
study conducted by Wu et al., soybean aphid populations peaked in late vegetative to

early reproductive stages with a second smaller peak as plants neared physiological

maturity. Generation times range fiom 2 to 16 days and decline with temperature (Li et
al. 2000). Wingless and winged female aphids produce an average of 58 and 38 nymphs,
respectively, at 26°C (Li et al. 2000). Winged females (gynoparae) and males appear in
the fall as the temperature decreases and plant conditions deteriorate. They migrate to
buckthom where the gynoparae produce Wingless females called oviparae. The males
mate with the oviparae, which lay over-wintering eggs. The life cycle repeats the next
spring. Winged aphids play a vital role in expanding the range of dispersal within and
among fields and in migration between alternative host plants. Crowding of Wingless
adults and poor host quality induce winged aphid production (Lu and Chen 1993).

In many parts of the North Central United States, e. g., northern Minnesota, North
Dakota, and Ontario, Canada, A. glycines is typically not found before early July,
suggesting that these fields are colonized by winged individuals that emigrate from other
soybean fields rather than by individuals that come directly from over-wintering hosts.
When soybean is in vegetative growth, soybean aphid colonies are commonly found at
the growing points, e.g., partially expanded young trifoliate, petioles, and stems. When
plants begin to flower, set pods, and develop lateral branches, soybean aphids tend to
become more widely dispersed on the plant and can be found on the underside of mature
leaves, on lower stems, lateral branches, petioles, and pods. Dense colonies are
sometimes tended by ants.

The movement to and from summer and Winter hosts seems to be a biological
“bottleneck” that has the potential to limit the ability of the soybean aphid to over winter
successfully. Fall migration can be challenged by crop phenology. In some years, much

of the soybean is harvested by the mid-September. These fields would have begun

senescing and dropping leaves sometime before harvest, so only aphids colonizing stems
and pods would survive until those parts of the plant are too desiccated to support further
development. If soybean senescence precedes the development of gynoparae, the females
that migrate from secondary to primary hosts to produce the sexual females and males,
there is little possibility of over-wintering by the aphid in that area. Late-planted or
double-cropped soybean may be the primary source of gynoparae and males. Data
indicate that the soybean aphid seems to have adapted to the North Central United States.
It has expanded its host range to use local Rhamnus species as over-wintering hosts. The
aphid has also overcome challenges to synchronize its life cycle with the senescence of
soybean in the fall and the relative late emergence of the crop in the Spring (Wu et al.,
2004). Several factors affect outbreaks of soybean aphids including environmental
factors, over-wintering success, cultural practices, natural enemies, and the
synchronization of soybean and aphid development (Wu et al. 1999). Rainy weather
helps to reduce populations of soybean aphid. Raindrops may knock aphids off plants and
high humidity increases the chance for fungal diseases to infect the insects (Wu et al.
2004)
Symptoms and Damage

The soybean aphid is the only aphid in North America that develops large
colonies on soybeans. Plant damage occurs when large numbers of aphids remove
significant amounts of water and nutrients as they feed on leaves and stems, causing
leaves to wilt, curl, yellow, and even drop. Other symptoms of direct feeding damage
include plant stunting, poor pod fill, reduced pod and seed counts, smaller seed sizes, and

nutrient deficiencies, resulting in overall yield and quality reduction (DiFonzo and Hines,

2002). Significant yield loss (8-25%) occurs when aphid densities peak at flower
initiation. Honeydew, a sticky substance excreted by soybean aphids onto the leaves,
leads to the development of sooty mold, which affects photosynthesis and results in yield
and seed quality loss (Chen and Yu 1988). During the feeding process, soybean aphids
are capable of transmitting viruses including soybean mosaic, alfalfa mosaic, mungbean
mosaic, peanut mosaic, and bean yellow mosaic virus (CAB International 2001). These
viruses commonly occur together and form a disease complex that leads to plant stunting,
leaf distortion and mottling, reduced pod numbers and seed discoloration (Glogoza
2002). Soybean mosaic virus (SMV), transmitted in a nonpersistent manner and causing
high yield loss, is spread mainly by aphid feeding. Epidemics of SMV are dependent not
only on the initial virus source but also on the abundance and development of aphid
vectors. Occurrence of winged aphids in soybean fields is closely associated with the
incidence of SMV (Quimio and Calilung 1993).
HOST PLANT RESISTANT MECHANISMS

Resistant varieties of crop plants play an important part in controlling many
insect, mite and nematode pests. When resistant varieties are used, fewer applications of
insecticides are usually needed to control insect pests than when susceptible varieties are
planted. Parasites and predators can be more effective on resistant than on susceptible
varieties. For instance, the European corn borer is more effectively controlled by a
protozoan parasite, Nosemia pyrausta, on partially resistant maize varieties than on
susceptible plants. Pesticides are too expensive to use on low value crops. It is therefore
often very desirable to develop resistant varieties that do not need to be supported by

chemical control measures (Russell 1978).

From an economic point of view, the use of insect-resistant cultivars in pest
management programs offers growers the advantage of genetically incorporated insect
control for the cost of the seed alone (Renwick, J. 1983). Resistance to insects is
composed of genetically inherited qualities that result in a plant of one cultivar or Species
being less damaged than a susceptible plant, which lacks these qualities. Plant resistance
to insects is always relative and the degree of insect resistance is based on comparison to
the susceptible plants that are more severely damaged under Similar test conditions. This
practice is necessary, since resistance is influenced by environmental fluctuations
occurring over both time and space. The importance of developing crop plants that are
resistant to major insect pest has created the need for examination of the mechanism
involved in resistance. The widely recognized classification proposed by Painter (1951),
provides an acceptable breakdown of the possible bases of resistance: nonpreference,
antibiosis, and tolerance. Nonpreference refers to behavioral responses of insects to a
plant, whereas antibiosis and tolerance refer to plant characteristics. Kogan and Ortman
(1978) suggested the term antixenosis to describe the plant properties responsible for
nonpreference.

According to Smith (1989), if a plant deters feeding by an insect, the mechanism
of resistance may be either antixenosis or antibiosis. The main difference between these
two types of resistance is whether the insect is completely prevented fi'om feeding, thus
starving to death (antibiosis) or will eventually feed on that plant when given no choice
(antixenosis). Often there is an overlap between the antibiosis and antixenosis types of
resistance. The choice of host plants is affected by a vast array of positive and negative

factors. Positive factors include physical stimuli, attractants, feeding stimulants and

oviposition stimulants. Negative factors include physical barriers, repellents and feeding
deterrents. Whether a potential host plant is selected by an insect often depends on a
delicate balance that may be tipped in either direction by the presence or absence of one
of these factors.

Complex types of resistance and combinations of different types of resistance can
be expected to give more complete and durable control than one Simple type. For
example, a resistant variety that expresses antibiosis and tolerance to an insect will give
an excellent and durable control. On the other hand, such a combination might be
difficult to select for and manage in a breeding program.

Antixenosis or nonpreference

Antixenosis refers to the undesirability of plant to an insect, resulting in
avoidance by the insect. Both antixenosis and non-preference denote the presence of
morphological or chemical plant factors that adversely change insect behavior, resulting
in selecting of an alternative host plant. Physical barriers such as thickened plant
epidermal layers, waxy coatings on leaves and stems or trichomes may force insects to
abandon their efforts to feed on an otherwise palatable host plant. Antixenosis in plants
may also be due to the possession of unique phytochemicals that repel or deter insect
herbivores from feeding or oviposition.

Antibiosis

Antibiosis refers to the unsuitability of a plant as food. This is the type of plant
resistance to insects that describes the negative effects of a resistant plant on the biology
of an insect attempting to use that plant as a host. Both chemical and morphological plant

defenses mediate antibiosis, and the effects on resistant plants range from mild to lethal.

A chronic effect of antibiosis leads to mortality in older larvae, pupa and adults.
Individuals surviving the. direct effects of antibiosis may also suffer reduced body size
and weight, prolong periods of development in the immature stages and reduced
fecundity as surviving adults. Antibiosis occurs because of the presence of plant
allomones or the absence of plant kairomones. Resistant cultivars may lack the proper
quantities of basic insect nutrients or contain phytochemicals that are toxic to insects, or
have high concentrations of structural plant substances such as 1i gnin and silica that
reduce insect digestion.
Tolerance

The tolerance mechanism of resistance is measured by the ability of plants to
withstand or recover from damage caused by insect infestation, which is equivalent to
what is required to damage a susceptible cultivar. Tolerance is determined by inherent
genetic capability to outgrow or recover after insect damage. From an agronomic point of
view, the plants of a tolerant cultivar have higher yield. Tolerance ofien occurs in
combination with antixenosis and antibiosis components of resistance.
SCREENING FOR INSECT RESISTANCE

Screening for insect resistance under natural conditions is a long-term process. In
the early stages of a plant resistance program, field populations of pest insects are
normally used by researchers to evaluate plant materials. However, field evaluation of
plant materials has some problems that may affect the search for resistance. Differences
in insect populations over space and time makes it difficult to identify reliable and stable

sources of resistance in natural conditions. It is therefore necessary to develop choice and

10

no-choice screening techniques where the test cultivars can be subjected to a uniform
insect pressure (Ananthakrishnan 2001).

Caging insects with test plants is one of the most dependable methods of
screening for insect resistance. It is possible to maintain uniform insect pressure on test
entries and also test at the same stage of development. Not only does the cage keep the
insect from migrating it also keeps natural enemies away from the insects. Caging tests
can be carried out under field or greenhouse conditions, and the cages Can be developed

to cover whole plants or plant parts (Smith 1989 and Smith et al. 1994).

11

MATERIALS AND METHODS

PLANT MATERIALS

Soybean plant introductions (PI) from MG 0 to 111 were obtained from the USDA
Soybean Germplasm Collection in Urbana, Illinois. A total of 2,147 PIs were screened in
2002 and 2003 (Appendix A and B). Accessions originally from northern China were
selected, as the climatic conditions are similar to those in the northern USA, where the
soybean aphid over winters. ‘Williams 82’ (Appendix A, Entry 1047) was included as a
susceptible check and three resistant genotypes, ‘Dowling’, ‘Jackson’, and PI 71506
(Appendix B, Entries 915, 1107, 1108) (G. Hartman, personal communication, 2002 and
2003), were included as resistant checks (Hill et a1. 2004). Both the susceptible and
resistant checks were obtained from Dr. Glen Hartman, USDA-ARS at Urbana, Illinois.

All PIS and checks were first evaluated in choice tests (Davis 1985) in which the
aphid fed on the genotypes they preferred to identify PIS possessing either antixenosis or
antibiosis. The resistant PIs were then re-evaluated in no-choice tests (Davis 1985) in
which aphids were confined on plants to identify PIS with antibiosis resistance.
LOCATION OF EXPERIMENT

All experiments were conducted at Michigan State University (MSU), East
Lansing, MI. Soybean aphids were obtained from nearby naturally infested soybean
fields for summer fieldwork, and from a colony maintained in grth chambers at the
Field Crops Entomology Laboratory at MSU for winter greenhouse work. The

experiments were set up as a randomized complete block design with two replications.

12

SCREENING OF GERMPLASM
Summer Field Screening - Choice Test

Two experiments were carried out in 2002 and 2003 to screen soybean germplasm
for aphid resistance. Summer plantings were done at the Agronomy Farm, MSU, in 12.2
x 18.3 m polypropylene cages (Redwood Empire Awning Co., Santa Rosa, CA), that are
aphid-, and predator - proof.

In 2002, 1,043 PIS, a susceptible check Williams 82, and an anonymous resistant
check provided by Dr. Glen Hartman at the USDA-ARS at Urbana-Champaign were
evaluated. Five seeds were planted per accession, for a maximum of five plants per
accession afier emergence. The plot length for each accession was 0.30 m long. After
emergence of the first trifoliate, two plants per accession were inoculated with two aphids
each, using a camel-hair brush. Aphids were obtained from naturally infested fields on
the agronomy farm. The aphids were left to multiply and move among plants. The plants
were removed from the cage at the end of the experiment; the experiment was re-
randomized and re-planted. Infestation was done as described for the first planting.

In 2003, 1,103 PIS, three resistafnt checks Dowling, Jackson, and PI 71506 and a
susceptible check Williams 82 were evaluated in two field cages simultaneously. The
method of inoculation, plot sizes, and evaluation procedures were the same as for the
2002 field screening.

Two experiments were carried out in 2002 and 2003 to screen soybean germplasm
for aphid resistance. Summer plantings were done at the Agronomy Farm, MSU, in 12.2
x 18.3 m polypropylene cages (Redwood Empire Awning Co., Santa Rosa, CA) that are .

aphid- and predator- proof.

13

In 2002, 1,043 PIS, a susceptible check Williams 82, and an anonymous resistant
check provided by Dr. Glen Hartman, USDA-ARS at Urbana, Illinois, were evaluated in
a field cage. The PIS and checks were planted on June 26. Five seeds per accession were
planted in a plot 0.30 m long. After emergence of the first trifoliate, two plants per
accession were inoculated with two aphids each using a camel-hair brush. Aphids were
obtained from naturally infested fields on the Agronomy Farm, MSU. The aphids were
lefl to multiply and move among plants.

In 2003, a new set of 1,103 PIS, three resistant checks Dowling, Jackson, and PI
71506 and a susceptible check Williams 82 were evaluated in two field cages. In each
cage, a complete set of the PIS plus the checks were planted as a randomized complete
block, which is considered as one replication of the test. The planting was carried on May
30 in one cage and on June 6 in the other cage. The methods of inoculation plot Sizes, and
evaluation procedures were the same as for the 2002 field screening.

Winter Greenhouse Screening - Choice Test

A winter screening was carried out in a large greenhouse with temperatures

between 22 and 25 ° C to verify the results obtained in the field in 2002. Winter planting
was conducted on November 21 of 2002 in the greenhouses at the Horticulture Research
Farm at MSU. Three plants of a genotype were planted as a plot in a plastic pot 22 cm in
diameter and 23 cm deep. Two of the three plants were inoculated with two aphids each.
Each greenhouse bench held 25 plastic pots.
Winter Greenhouse Screening-No-Choice Test

A no-choice test was carried out in the greenhouse from December 2003

to February in 2004, to determine the type of resistance of each resistant source. Each

14

plot was set up as described for the 2002 greenhouse plantings. Each plot was isolated by
the use of a fine nylon net that was held in position over the plastic pot by a foot tomato
cage. The entries in the no-choice test were the resistant PIS identified in the 2002 and
2003 screening in the choice test, a resistant check Jackson, a susceptible check Williams
82, and two soybean varieties ‘Titan’ and ‘Loda’.
CONFIRMATION OF RESISTANCE

In the summer of 2004, the six PIS identified as potentially resistant to the aphid
after two years of screening and a susceptible check (Williams 82) were evaluated to
confirm the resistance. The experiment was set up as a randomized complete block
design with three technical replications. Ten plants were planted per 0.6 m plot for each
entry. All ten plants were inoculated as described earlier. Ten plants were planted per 0.6
m plot for each entry. All ten plants were inoculated as described earlier. 7
DATA COLLECTION
In all studies, except the confirmation of resistance test, aphid populations on inoculated
trifoliates were counted 10 days after inoculation. Four weeks after inoculation, the
accessions were visually rated for susceptibility to soybean aphid using the method of
Zhuang (1999). Each plant in a plot was visually rated using the scale shown in Fig. 1. A
damage index (DI) for each accession was calculated using the following formula
(Zhuang 1999): D1 = 2 (Scale value x No. of plants in the category)/ (4 x Total no. of
plants evaluated) x 100. The DI can range between 0% for no infestation and 100% for
the most severe damage. A D1 of 30% or less was classified as resistant, whereas a D1 of

30% or more was classified as susceptible. In the second year of field screening the plants

15

were visually rated weekly for a month beginning at 3 weeks afier inoculation and DI
value at week 4 after inoculation was used to determine susceptibility of PIS.
Statistical Analysis

The data for each year were analyzed using the PROC GLM procedure in the SAS
statistical package V8 (SAS Institute, 1999). Means were separated by least significant
difference (LSD) at the 5% probability level. Linear correlations between the average
number of aphids per leaflet ten days afier inoculation and the DI were calculated with

PROC CORR.

16

      

I

Figure 1: Illustration of the visual rating scale to esablits the Damagrgn Index
(DI). 0 =No aphids, plant appears normal and healthy; 1=Less than 100 aphids per plant,
plant appears normal and healthy; 2:101 — 300 aphids per plant, mostly on the young
leaves and the tender stem at top of plant; 3:301— 800 aphids per plant, leaves slightly
curly and shiny, young leaves and stems covered with aphids; 4=More than 800 aphids
per plant; plants stunted, leaves severely curled, yellow, covered with sooty mold and

cast skins.(lmages in this thesis /dissertation are presented in color)

17

RESULTS

CHOICE TEST IN 2002

. In the first year of screening in the seed cage the average number of aphids
per leaflet ranged from O to 500. In the greenhouse the average number of aphids per
leaflet ranged from 0 to 170. Results from the visual rating and calculation of the DI
(Figure 2) showed that 1008 (99.3%) and 973 (99.1%) of the accessions screened in the
field and greenhouse, respectively, were susceptible to the soybean aphid (DI > 30%).
The correlation between the average number of aphids per leaflet and the D1 of an
accession was low (r = 0.165, n=1017, p< 0.0001). Seven of the 1043 accessions
appeared to have some resistance (DI<30%) to the aphid in the seed cage, while eight
accessions showed some resistance in the greenhouse (Table 1). Only three of these
accessions were resistant in both the field and greenhouse screenings. The accessions
which did not Show resistance in both the field and greenhouse were replanted in the
greenhouse in the spring of 2003 and found to be escapes or susceptible. Afier the first
year of screening, P1567543C, PIS67597C, and P15675418 (Appendix A, Entries 775,
779, 810) appeared to be resistant to the soybean aphid. PI 5675988 (Appendix A, Entry
812) was also selected for further study because it had a D1 of 25% in the summer despite

a D1 of 33.3% in the winter.

18

2002 Damage Index for field and

 

Greenhouse
1000~.,,W,,,-, L942,
‘ g 9007
'53 800 < 746
(I)
A 8 700
o .
C6 1
«L 600
‘ O
S 500
.D
E 400 .
3
. Z 300
200
200 .
56
100 7 8 10 27
0 E 7‘2. 7, i
25 50 75 100
Damage Index (%) IField DGreenhouse

Figure 2:Damage Index categories for choice test in 2002 conducted in the field in
summer and in greenhouse in Winter at Michigan State University

CHOICE TEST IN 2003

In the second year of screening, the number of aphids per leaflet ranged from O to
326 for the first replication and 0 to 244 for the second replication. Based on D1 931
(98.7 %) and 995 (99.0 %) of the plants were found to be susceptible (DI>30%) in
replication 1 and 2 respectively (Figure 3). As in the previous year, this DI did not reflect
what was obtained by counting the aphid population. The correlation between the average
number of aphids per leaflet and the final D1 of each accession was again low (I =0.20,
n=1108, p<0.0001).
In the first replication, eight accessions performed Similar to the three resistant checks. In
the second replication, ten accessions performed similar to the three resistant checks
(Table 1). The difference in numbers of resistant accessions was due to some accessions
failing to germinate in either cage. However there were only two accessions, P1603392
and PI603418C (Appendix B, Entries 1036 and ‘1049), which had a D1 of 25% in both
replications. In cases where germination did not occur we replanted in the greenhouse to
confirm their resistance, but they were found to be susceptible and were possibly escapes.

Weekly visual rating using the method of Zhuang, (1999) showed that there was a
clear difference in susceptibility or resistance among accessions four weeks after
inoculation, when aphid densities reached its peak.
NO-CHOICE TEST

The six resistant PIS identified in 2002 and 2003 choice tests, PI 567543C,

PI 567597C, PI 5675418, PI 5675988, PI 603392, and PI 603418C, were evaluated for
aphid resistance in a no-choice test. The no-choice study revealed that two of the six

resistant PIS, PI 5675418 and PI 5675988, had significantly lower DIS than the other

20

entries in the test. The effect these two PIS had on the aphids was similar to that of
Jackson, the resistant check (Table 2). There was a high correlation (r = 0.63, p = 0.05)
between the average number of aphids per leaflet 10 days afier inoculation and the DI an
entry had. The other resistant PIS and Titan had significantly (p $0.05) higher damage
indices than these two PIS, but were also significantly different from Loda and Williams

82, the susceptible check which had the highest DI.

 

2003 Damage Index for Rep.1 and Rep. 2

 

 

 

 

 

 

 

 

900 856 i
800~4 753 '
E”
,9 700-
8
a) 6004
o
o
‘3 500
‘8
g... 400‘
B
E 300-
3 200- 164
Z “127
1 . r
00 12 10 14 12
OS

25 50 75 -m,199_,-._-_ __
Damage Index (%) Cilia} £3 Esp; T

Figure 3: Damage Index categories for choice test in 2003 conducted in summer in
two simultaneous replications at Michigan State University

21

Table 1: Number of accessions in each Dl category for 2002 (n = 1043) and 2003 (n=

 

 

 

1108).
Year and _ Damage Index
Loca‘iW’eplica‘im s 30% 31 t050% 51 to75% > 75%
Field (2002) 7 10 56 942
Greenhouse (2002) 8 27 200 746
Field Rep. 1(2003) 12 14 164 753
Field Rep. 2 (2003) 10 12 127 856

 

22

Table 2: Results of the no-choice test for the Six resistant accessions, resistant and
susceptible checks, and varieties in 2003.

 

Entry Average NO. of aphids Damage

 

Maturity group per leaflet ‘1' index (%) I
P1567543C HI 8 56b§
P1567597C III . 1 62 b
P15675418 III 1 25a
P15675988 III 1 1 25a
P1603392 III 5 81 c
P1603418C III 11 77 c
Jackson VII 2 25a
Titan 1 17 71 c
Loda II 19 83 (1
Williams 82 III 19 100 (1
Mean 9.4 60.5

 

TThe data are the averages of 12 leaflets from two replications with two plants per
replication and three leaflets per plant taken 10 days after inoculation.

IAverages of two replications.

§ Means followed by the same letters are not Significantly different by the least
significant difference test (p=0.05)

23

CONFIRMATION OF RESISTANCE

Resistance in the four accessions, (P1567543C, PIS67597C, P15675418, and PI
5675988) identified in the choice tests conducted in 2002 was confirmed (Table 3).
Three and four weeks after inoculation, respectively, highly significant differences (p<
0.0001) were seen between the DIS for these four accessions and the two accessions,
P1603392 and P160341 8C, obtained from choice tests conducted in 2003. The amount of
damage to the plant as a result of aphid feeding was greater on the susceptible check than
P1603392 and P160341 8C four weeks afier inoculation. The susceptible check had curled
leaves, appeared stunted, and its leaves were covered with black sooty mold while PI

603392 and PI 603418C Showed none of these symptoms.

24

Table 3: Damage Index (DI) based on three replications in 2004 for six putative resistant
accessions, identified after two years of screening, and a susceptible check three and four
weeks afler inoculation.

 

 

 

Damage Index (%)
Accession Three weeks after Four weeks afler
or cultivar inoculation inoculation
P1567543C 25 at 25 a
» P1567597C 26 a 26 a
P15675418 25 a 25 a
P15675988 26 a 26 a
P1603392 75 b 79 b
P1603418C 75 b 79 b
‘Williams 82’ 83 c 100 c
Mean 46.86 51.43

 

1' Mean of three replications of a maximum of 10 plants each. Means followed by the
same letters are not significantly different by the least significant difference test (P=0.05)

25

DISCUSSION

In the USA, research on screening for soybean germplasm resistant to the soybean
aphid began as soon as the pest was discovered. Hill et al. (2004) screened 1,542 soybean
genotypes, mostly current North American soybean cultivars, and found resistance in
three North American soybean ancestral lines: Dowling, Jackson, and PI 71506. These
resistant genotypes belong to MG IV to VIII that are not well adapted to the areas of the
northern US where soybean aphids are most prevalent. In our study, .we identified four
resistant accessions (PI 567543C, PI 567597C, PI 5675418, and PI 5675988) belonging
to MG HI afier screening 2,147 soybean accessions in MG 0 to 111. All of these primitive
Chinese cultivars originated from Shandong province. These resistant accessions can be
readily incorporated into the elite soybean germplasm for the north central States.

Lin et al. (1992) showed that the soybean aphid colonizes soybeans at the early
vegetative stage. Aphid populations increase gradually until a 10 to 15 day exponential
grth phase from late vegetative to early reproductive stage of the plants. It is not
surprising that 10 days after inoculation, at the early vegetative stage, a high percentage
of our test plants had very few aphids per leaflet. Correlations were low between the
number of aphids per leaflet 10 days afler inoculation and the DI four week after
inoculation in the first and the second years of screening (r=0.16 and F012,
respectively). These low correlation values indicate that counting aphids 10 days afier
inoculating in the early vegetative stage is not an optimal method for determining the
possible resistance or susceptibility of an accession. It would be advisable to count aphids
in the late vegetative or early reproductive stage in order to identify truly resistant

accessions. However, counting aphids is very tedious and time consuming. For large-

26

scale evaluation of aphid resistance such as progeny evaluation in a breeding program,
the method of Zhuang (1999) is more appropriate.

The six resistant accessions [PI 567543C, PI 567597C, PI 5675418, P1 5675988,
PI 603392 and PI 603418C] identified in field and greenhouse choice tests are in MG 111.
These accessions possess either antixenosis or antibiosis (Smith et al. 1994). The no-
choice test showed that PI 5675418 and PI 5675988 had adverse effects on aphid
longevity and fecundity and thus possessed antibiosis according to Painter (.1951). The
high DIS obtained in no-choice test for PI 567543C, PI 567597C, PI 603392, and PI
603418C which were hitherto resistant in choice tests is likely due to different feeding
responses of the aphid between choice and no-choice tests as found by Smith et a1.
(1994). Also it is possible for a genotype classified as resistant in a choice test to be
declared susceptible in a no-choice test (Tingey, 1986). In the no-choice test there was a
high (r = 0.63, p= 0.05) correlation between the average number of aphids per leaflet 10
days after inoculation and the D1 an entry bad. This high correlation could be attributed to
the fact that the entries chosen for this test were truly susceptible or resistant as found in
previous screenings.

The test conducted to confirm the resistance found after two years of screening
revealed that PI 603392 and PI 603418C, were not resistant to the soybean aphid.
Observing the plants after the aphid population had declined due to lower temperatures
and reduced photoperiod, PI 603392 and PI 603418C did not Show any of the signs
associated with severe aphid infestations. According to Painter (1951), the type of
resistance that enables a host plant to withstand infestation by insects without suffering

severe damage is tolerance. P1 603392 and PI 603418C might be tolerant but

27

this can only be confirmed with further yield and dry matter studies. These two
accessions were not considered resistant after their poor performance in the confirmation
test. Smith (1989) also observed that pseudo-resistance or false resistance may occur in
normally susceptible plants. Resistance may have been induced temporarily by variations
in temperature, day length, soil chemistry, plant or soil water content, orinternal plant
metabolism. Also susceptible plants may simply escape damage because of incomplete
infestation.

This research is the first report of soybean aphid resistance in early maturing
soybean germplasm. Further genetic studies are necessary to understand the genetics
underlying the resistance that we have found. Genetic studies will expedite the transfer of
this resistance into cultivated varieties. These new sources of resistance would be utilized
in our breeding program to develop soybean resistant varieties suitable for the North

Central States of the USA and would greatly benefit the soybean industry.

28

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31

APPENDIX

32

APPENDIX A

Germplasm used and data collected in 2003

33

 

 

 

Replication] Replicationz
Entry PU FC N r N 1
number MAX MEAN °' ° DI MAX MEAN °' ° [)1
plants plants

1 PI 548298 20 10 2 100% 65 53 1 100%
2 P1548316 12 8 2 88% 82 72 2 100%
3 PI 548317 - 9 0 - - - 0 -

4 PI 543818 0 - 0 - 92 25 4 100%
5 PI 548333 12 4 O - 60 47 3 100%
6 PI 548340 57 42 2 100% 46 - 1 100%
7 PI 548348 41 25 2 88% 18 5 2 75%
8 PI 548362 60 53 3 83% 52 ‘ 35 2 100%
9 PI 548365 15 10 2 88% 41 22 3 100%
10 PI 548366 18 9 2 88% 40 - 1 100%
11 PI 548367 12 9 3 92% 18 - 1 100%
12 PI 548373 45 21 2 100% 50 - 1 100%
13 PI 548376 0 - 0 - 18 - 1 75%
14 PI 548377 0 - O - - - O -
15 PI 548381 10 7 1 100% - - 0 -
16 PI 548383 0 - 0 - 32 - 1 100%
17 PI 548388 101 80 1 100% 50 - 1 100%
18 PI 548403 153 95 O - 82 - 1 100%
19 PI 548412 60 51 1 100% 101 86 3 100%
20 PI 548421 89 66 2 88% 82 - l 100%
21 PI 548432 53 14 3 83% 92 54 3 92%
22 FC 2108 107 88 3 83% 72 52 3 100%
23 FC 2109 40 25 3 100% 42 31 2 88%
24 FC 3609 81 7O 2 88% 60 49 5 85%
25 FC 3654N 96 67 2 88% 21 13 3 100%
26 FC 31571 176 111 1 100% 43 - 1 ' 100%
27 FC 31572-3 0 - 0 - 109 88 3 100%
28 PI 54583 183 133 3 100% 150 100 5 90%
29 PI 54591 93 75 2 88% 115 83 2 100%
30 PI 54592 0 - O - 80 39 5 90%
31 PI 54608-2 51 45 2 88% 56 26 2 100%
32 PI 54608-3 0 - 0 - - - O -
33 PI 54608-5 91 78 3 100% 132 88 3 100%
34 PI 54609 90 78 3 100% 40 34 3 83%
35 PI 54610-1 0 - 0 - - - 0 -
36 Pl 54613 96 67 4 94% 46 33 2 100%
37 P154615 10 6 1 75% 78 - 1 100%
38 PI 54615-1 40 34 2 88% - - 0 -
39 PI 54618 120 106 4 94% - - 0 -
40 PI 54620 79 57 2 100% - - 0 -
41 PI 54620-2 30 22 2 100% 74 - 1 100%
42 PI 54809 18 13 1 75% 38 20 2 88%
43 PI 54834 26 12 4 94% 75 6 2 100%
44 Pl 54853 20 10 4 88% 102 38 3 92%
45 PI 54854 24 16 4 88% 16 9 3 92%

 

Pl: Pant Intoduction, MG: Maturity Group, MAX: Maximum Number of Aphids per Leaflet

DI: Damaging Index, Mean: Average Number of Aphids on three inoculated leaflets

34

 

 

 

Replication] Replicationz
Entry PI] FC N f N 1
number MAx MEAN °' ° DI MAX MEAN °° ° D1
plants plants

46 PI 54857 53 42 4 88% 40 19 3 100%
47 PI 54865 20 10 5 95% 49 2 75%
48 PI 57334 12 10 4 88% 106 30 92%
49 PI 61940 16 11 1 75% 24 14 53 100%
50 PI 62202 92 28 5 100% 101 63 4 94%
51 PI 62483 57 44 2 88% 41 21 3 92%
52 PI 6327] 23 19 3 100% 61 41 2 100%
53 PI 65379 59 16 3 100% 88 51 2 100%
54 PI 68423 53 44 3 100% 65 52 2 100%
55 PI 68470 62 56 4 100% 51 40 4 100%
56 PI 68474-2 46 20 2 100% 61 3O 4 88%
57 P168479 12 8 l 75% 31 19 4 100%
58 PI 68479-1 41 31 2 100% 51 23 3 92%
59 PI 68483 18 10 2 75% 41 31 4 94%
60 PI 68498 14 12 1 75% 78 37 5 90%
61 PI68521-1 12 9 2 63% 42 - l 100%
62 P168523 20 14 2 88% 40 34 5 100%
63 P168528 1 0 4 4 94% 33 20 3 92%
64 P168530-2 14 5 4 100% 2 1 1 3 4 100%
65 PI68533-1 40 9 3 100% 52 23 5 95%
66 PI68533-2 40 25 3 83% 101 33 3 92%
67 P168535 30 11 3 100% 60 19 3 92%
68 PI68551-3 0 - 0 - - - 0 -

69 P168554 40 1 1 3 100% 20 3 2 88%
70 P168560 85 5 1 3 100% 32 23 2 100%
7 1 P168576 84 7 1 5 90% 8 1 7O 3 100%
72 P168586 12 8 2 75% 65 47 4 100%
73 P168599 14 1 1 1 75% 1 1 1 58 4 94%
74 PI68604-1 15 10 4 81% 86 81 5 100%
75 PI68604-2 14 8 3 75% 52 32 4 75%
76 PI68609A l 2 9 5 95% 8 - 1 100%
77 P168621 14 8 4 88% O 0 3 83%
78 P168648 38 28 4 94% 71 - 1 100%
79 P168679 97 10 3 83% 21 - 1 100%
80 Pl68692-2 44 34 3 100% 1 5 - 1 1 00%
81 P168701 120 84 3 100% 81 14 2 88%
82 P168710 69 56 5 90% 8 5 3 100%
83 P168731 33 24 5 90% 9 1 4 100%
84 P168732-l 0 - O - - - 0 -

85 P168746 0 - 0 - - - 0 -

86 P168748-1 104 l 1 4 94% 9 6 5 75%
87 P168756 61 50 2 100% 94 38 3 92%
88 P168759 52 43 1 75% 6 - 1 75%
89 PI68761-3 121 92 4 88% 38 14 3 92%
90 P168806 92 79 2 100% 14 5 3 100%

 

35

 

 

 

Replication] ReplicationZ
Entry PI, FC N I N 1
number MAX MEAN °° ° D1 MAX MEAN °' ° DI
plants plants

91 P169507 150 121 2 100% 24 18 3 75%
92 P169515 180 128 1 100% 8 5 5 100%
93 P169533 120 92 4 94% 51 10 2 75%
94 P169993 158 1 1 4 100% 51 20 2 75%
95 P169995 180 1 12 1 100% 9 6 3 75%
96 P170001 101 43 3 100% 21 3 2 75%
97 P170014 147 108 4 94% 21 13 3 67%
98 P170019 83 73 2 88% 21 13 3 67%
99 P170023 52 39 1 100% 0 0 3 75%
100 P170076 90 72 3 100% 20 2 2 75%
101 P170080 53 44 2 100% 10 8 3 92%
102 P170188 38 23 3 100% 12 7 2 100%
103 P170189 128 111 2 100% 21 13 3 67%
104 P170192 0 - 0 - 8 - 1 100%
105 P170199 151 126 1 100% ' 59 7 2 100%
106 P170201 120 100 3 92% 66 37 3 92%
107 P170202 217 139 1 100% 94 41 4 100%
108 P170212 61 38 2 88% 1 0 4 94%
109 P170213 20 18 1 100% 35 26 2 100%
110 P170247 90 82 2 100% 68 57 2 100%
1 1 1 P170253 81 66 4 100% 20 9 5 70%
1 12 P170462 20 17 2 88% 61 17 3 92%
113 P1704664 161 48 2 88% 58 24 3 100%
1 14 P170469 0 - 0 - 32 20 5 100%
1 15 PI70469-1 30 18 4 100% 26 - 2 88%
1 16 P170470 128 109 3 100% - - o -

1 17 P170471 33 24 4 88% 55 42 5 95%
1 18 P170473 22 10 4 100% 50 42 4 94%
1 19 PI70473-1 40 25 4 88% 63 - 1 100%
120 P170500 0 - 0 - - - 0 -

121 P170501 26 20 4 94% 20 13 2 100%
122 P170515 20 13 4 94% 13 5 3 92%
123 P170519 42 37 3 92% 15 - 1 100%
124 P170528 28 20 1 75% 24 21 5 95%
125 P170541 206 194 3 100% 1 1 - 1 100%
126 P170559 81 40 3 92% 24 - 1 100%
127 P170566 0 - 3 100% o - 1 75%
128 P171161 70 15 4 94% 130 86 5 95%
129 P171845 20 7 2 88% 26 18 2 100%
130 P17150-1 181 66 3 100% 50 27 2 88%
131 P179583 75 33 4 75% 52 18 2 100%
132 P179587 12 10 3 83% 1 1 4 3 92%
133 P179616 40 32 1 75% 20 15 3 92%
134 P179617 19 13 2 100% 15 - 1 100%
135 P179620 41 34 5 100% 90 - 1 100%

 

36

 

 

 

Replication] Replication2
Entry “I FC N r N 1
number MAX MEAN °° ° DI MAX MEAN °° ° or
plants plants

136 P179627 48 35 5 75% 41 29 3 100%
137 P179628 111 103 2 75% 23 15 2 100%
138 P179645 6 0 4 75% 6 4 5 100%
139 P179648 0 - 0 - 12 9 4 88%
140 P179691 91 79 3 100% 61 - 1 100%
141 PI79691-4 72 57 3 92% 62 3o 4 100%
142 P179692 0 - 1 75% 41 16 4 100%
143 P179693 30 15 3 83% 30 12 2 88%
144 P179710 36 20 5 75% 38 22 1 100%
145 P179726 72 64 3 100% 24 14 5 100%
146 P179760 12 9 3 67% 93 48 2 88%
147 P179797 21 18 3 83% 59 38 3 92%
148 P179835 51 43 1 100% 19 1 1 2 100%
149 PI79848-1 61 55 3 92% 40 17 3 92%
150 PI79870—1 91 81 5 100% 77 19 4 100%
151 PI79870—2 82 66 2 88% 12 - 1 100%
152 P179872 31 24 3 92% 25 17 4 81%
153 P179874 20 14 1 75% 26 - 1 75%
154 PI79874-1 30 23 1 75% - - 0 -

155 P180822 12 10 4 88% 38 6 4 100%
156 P180831 14 9 5 90% 21 9 3 92%
157 P181667 66 53 3 100% 34 26 1 100%
158- P181765 106 52 5 100% 13 9 3 100%
159 P181766 11 4 3 75% 10 - 1 100%
160 P181772 22 18 5 85% 40 26 3 92%
161 P185668 59 38 3 75% 2 - 1 75%
162 P187531 18 5 5 50% 1 1 2 3 83%
163 P188282 26 15 4 69% 22 14 2 88%
164 P188287 17 12 3 83% 3 - 1 50%
165 P188289 22 1 1 4 63% 10 4 3 75%
166 P188290 14 7 3 58% 21 16 3 67%
167 P188291 18 12 3 92% 67 36 4 81%
168 P188292 32 19 5 80% 1 1 9 4 100%
169 P188295 14 9 1 100% 20 6 3 100%
170 P188297 157 95 3 100% 42 29 2 100%
171 P1883034 26 - 1 75% 2 0 3 92%
172 P188305 205 89 5 95% 37 21 5 100%
173 P188306 123 41 3 100% - - 0 -

174 P188306-1 163 89 3 100% 128 20 3 75%
175 P188310 144 107 3 100% 96 56 3 100%
176 P188312 86 72 3 100% 68 5 5 80%
177 P188349 80 64 2 100% 34 8 2 88%
178 P188350 81 69 4 100% 98 34 4 100%
179 P188353 140 8 2 100% 42 28 3 100%
180 P188354 109 56 3 92% 33 - 0 -

 

37

 

 

 

Replication] Replication2
Entry PU FC No of No of
number MAX MEAN ' DI MAX MEAN ° DI
plants plants

181 P188359 207 88 4 100% - - 0 -
1 82 P188443 1 23 65 5 100% - - 0 -
183 P188445 92 69 1 100% 62 50 3 67%
184 P188447 12 9 1 75% 60 23 2 75%
185 P188447-3 80 69 2 100% 120 14 3 83%
186 P188448 201 76 2 100% 29 21 2 75%
187 P188466 40 16 3 67% 18 1 1 3 58%
188 P188484 130 121 1 75% 0 - 1 25%
189 P188486 201 189 1 100% 6 2 3 67%
190 P188490-1 109 78 3 100% 18 13 2 100%
19] P188492 152 113 2 100% 20 13 3 75%
192 P188497 71 61 3 92% 9 5 4 100%
193 P188502 122 89 4 100% 6 3 2 100%
194 P188780 152 1 1 8 4 100% - - 0 -
195 P188782 260 132 3 100% 61 14 3 83%
196 P188783 80 69 3 83% 0 0 2 100%
197 P188788 82 60 3 100% 21 14 3 100%
198 P188789 166 96 4 100% 1 l 6 3 92%
199 P188790 0 - 0 - - - 0 -
200 P188797 121 77 2 100% 26 5 3 100%
20] P188799 112 94 1 100% 46 13 5 100%
202 P188803-1 62 58 2 100% 26 12 3 92%
203 P188804 79 50 2 100% 49 26 l 100%
204 PI88805—2 61 38 4 100% 45 29 5 90%
205 P188806 72 64 3 100% 53 38 2 63%
206 P188818 91 84 4 100% 34 25 2 50%
207 P188998-1 71 59 2 75% 121 91 3 58%
208 PI88998-2 71 61 2 100% 62 27 2 63%
209 P189002 90 3 1 3 100% 0 0 3 67%
210 PI89002-2 42 34 2 88% 27 14 4 88%
21 1 PI89003-2 82 31 4 100% 63 42 3 92%
212 P1890054 107 84 l 100% 30 10 4 75%
213 P189006 51 18 4 75% 20 2 3 92%
214 P189007 103 98 2 88% - - 0 -
215 P189009 60 47 3 92% 12 7 2 100%
216 P189009-2 92 74 3 100% 25 21 5 100%
217 P189012-] 0 - 0 - - - 0 -
218 P189055 87 75 4 88% 12 9 2 100%
219 P189056-3 3 5 24 3 100% 43 3 1 5 100%
220 P189057 64 49 1 100% 140 56 3 100%
22] P189058 250 65 3 92% 65 34 4 88%
222 P189060 226 191 3 100% 1 1 2 4 88%
223 P189061-1 30 29 1 100% 31 16 2 88%
224 P189061-2 0 - 0 - 0 - l 25%
225 P189061 N 99 71 3 100% 21 13 3 92%

 

38

 

 

 

‘ Replication] Replication2
Entry PU FC No of No of
number MAX MEAN ' DI MAX MEAN ' DI
plants plants

226 P189066 102 88 3 100% 58 10 2 88%
227 P189067 253 213 3 100% 98 77 3 100%
228 P189168 17 4 5 50% 100 69 2 100%
229 P189773 5 2 2 50% 12 6 3 92%
230 P189783 199 40 5 70% 79 64 5 90%
231 P189784 85 67 2 100% - - 0 -
232 P190407 0 - 0 - 49 34 3 100%
233 P190463 62 44 3 100% 105 18 5 100%
234 P190486 9 5 3 67% 27 1 1 4 100%
235 PI90499-l 96 72 1 100% 13 - 1 100%
236 P190566 40 34 2 100% 33 17 3 100%
237 P190566-1 41 24 5 85% 31 20 3 100%
238 PI90567-1 61 54 4 100% 0 0 5 70%
239 P190573 37 24 5 100% 19 1 1 5 95%
240 PI90576-1 0 - 1 100% 87 21 2 100%
241 P190578 90 75 2 100% 94 83 2 100%
242 P190579 48 36 1 75% - - 0 -
243 P191089 1 16 101 2 100% 277 173 5 80%
244 PI91100-1 30 22 1 75% 135 84 2 100%
245 PI91100-2 78 5 2 88% 225 — 1 100%
246 PI91100-3 98 87 2 100% 57 5 3 100%
247 P191108 N 16 9 2 75% 27 - 0 -
248 P191113 76 54 3 92% 29 16 4 100%
249 P191120 58 22 5 100% 32 18 5 85%
250 P191121 0 - 0 - 155 101 3 100%
251 P191127 20 18 1 75% 77 - 1 100%
252 P191142 92 84 2 100% 106 7 3 92%
253 P191149 150 136 2 100% 117 56 5 100%
254 P191151 78 54 2 100% 169 126 4 88%
255 P191152 98 53 4 100% 126 82 3 100%
256 P191153 0 - 0 - - - 0 -
257 P191154 0 - 0 - 0 - 1 75%
258 P191159 0 - 0 - 46 - 1 75%
259 P191160 0 - 0 - 72 59 2 88%
260 P191162 0 - 1 100% 13 10 1 25%
261 P191165 0 - 0 - 96 62 1 100%
262 P191169 0 - 0 - 43 33 5 100%
263 P191174 82 77 1 100% 55 33 3 67%
264 P191340 264 219 4 100% 82 - 1 100%
265 P191341 43 40 1 100% 46 - 1 75%
266 P191343 1 15 105 3 92% 123 32 3 92%
267 P191349 13 0 3 92% 8 5 2 100%
268 P191730 138 102 2 100% 46 33 2 75%
269 PI91730—1 0 - 0 - - - 0 -
270 P191731 89 34 2 100% 36 29 3 75%

 

39

 

 

 

Replication] Replication2
Entry PU FC N f N 1'
number MAX MEAN °' ° D1 MAX MEAN °° ° m
glants Jrlants

27] P192466 0 - 0 - 54 27 4 94%
272 P192560 77 45 4 100% 176 143 2 100%
273 P192568 0 - 0 - - - 0 -
274 P192577 0 - 0 - - - 0 -
275 P192590 0 - 0 - 63 - 0 -
276 P192593 57 43 4 100% 67 50 3 100%
277 P192594 0 - 0 - 130 - 1 75%
278 P192600 52 4 3 100% 5 3 34 3 100%
279 PI92601-1 28 24 3 92% 12 4 . 5 85%
280 PI92601-2 19 3 2 88% 79 65 3 100%
281 PI92601-5 37 30 2 88% 141 90 3 100%
282 P192602 0 - 0 - - - 0 -
283 P192605 105 37 4 100% 57 35 3 100%
284 P192606 62 1 1 3 92% 105 35 3 83%
285 P192608 84 18 3 92% 38 30 5 75%
286 P192617 0 - 0 - 0 - 1 100%
287 P192618 0 - O - 0 - 1 100%
288 P192619 0 - 0 - - - 0 -
289 P192623 14 12 1 75% 85 63 4 100%
290 P192640 0 - 0 - 5 4 3 100%
29] P192641 6 5 1 75% 43 37 3 100%
292 P192642 0 - 0 - 9 - 1 100%
293 P192643 32 18 2 75% 54 41 3 92%
294 P192645 19 13 2 25% - - 0 -
295 P192654 12 1 1 3 75% 139 50 3 100%
296 P192659 26 24 2 75% 155 1 19 4 100%
297 P192662 14 8 3 67% 35 - 1 100%
298 P192663 6 5 3 67% 49 6 4 88%
299 P192672 21 3 3 75% 73 26 5 100%
300 P192686 6 1 2 75% - - O -
301 P192688 91 11 3 92% 52 l 1 4 81%
302 P192690 58 43 1 100% 2 - l 100%
' 303 P192691 40 29 3 67% 17 6 3 100%
304 P192702 67 28 4 100% 19 10 2 100%
305 P192704 67 62 1 100% 12 6 4 100%
306 P192707 38 32 1 75% 18 10 4 100%
307 P192718 0 - 0 - 19 14 2 88%
308 P192718-2 O - 0 - 16 7 3 83%
309 P192720 141 52 2 100% 32 21 2 100%
310 P192722 142 124 2 100% 20 12 4 100%
31 1 P192728 0 - 0 - ‘ 34 9 5 95%
312 P193563 23 10 1 75% - - 0 -

3 13 P193565-A 65 3 8 3 100% 7 2 2 100%
314 P196193 0 - 0 - 49 32 3 100%
315 ”96194-3 74 35 2 88% 135 62 4 100%

 

40

 

 

 

Replication] Replication2
Entry PU FC N o of N 0 or
number MAX MEAN ’ D1 MAX MEAN ‘ DI
plants plants

316 P196199 25 20 3 67% 25 2 4 75%
317 P1123578 0 - 0 - - - 0 -
318 P117142] 42 36 1 100% 64 21 2 63%
319 P1189916 139 35 3 100% 148 109 3 58%
320 P1200593 42 20 1 100% 122 68 1 75%
321 P1200594 21 15 2 100% 45 27 2 50%
322 P12485098 1 7 8 3 92% 106 l 3 2 50%
323 PI253650A 43 1 8 l 100% 30 - 1 100%
324 P12536508 127 98 1 100% 49 - 1 75%
325 P1253651C 26 19 5 100% 48 31 2 63%
326 P1253653A 0 - 0 - - - 0 -
327 P1253660A 0 - 0 - - - 0 -
328 P12536608 44 38 1 100% 1 12 68 3 67%
329 PI253661A 105 26 2 100% 66 48 2 75%
330 P12536618 1 15 92 2 88% 123 69 4 69%
331 P1253665D 103 98 2 100% 102 17 2 75%
332 P1266807C 256 156 3 92% 85 18 2 100%
333 P1290126A 0 - 0 - 62 42 4 100%
3 34 P12901268 175 92 2 100% 69 14 5 90%
335 P1290134 O - 0 - - - 0 -
336 P12913068 78 61 3 100% 4 l 2 100%
337 P1297502 86 65 l 100% - - 0 -
338 P1297504 34 22 1 100% 14 1 1 5 90%
339 P1297505 106 86 1 100% 103 81 2 63%
340 P1297510 0 - O - - - 0 -
341 P1297511 79 65 4 100% - - 0 -
342 P1297514 72 67 4 100% 36 8 2 100%
343 P1297528 35 9 2 88% 199 172 5 100%
344 P1297533 124 78 2 100% 8 1 4 75%
345 P129754] 62 27 5 100% 44 36 3 100%
346 P1297543 41 26 2 88% 127 84 3 100%
347 P1297545 1 1 1 82 3 92% 1 1 - 1 100%
348 P1297548 86 2 2 100% 155 98 3 75%
349 P130365] 45 29 3 92% 6 5 3 92%
350 PI319535A O - 0 - 0 1 100%
351 P13195358 101 64 3 92% 9 4 88%
352 P1347560 0 - 0 - 57 - 1 100%
353 PI347565A 21 16 2 88% 5 - 1 75%
354 P1361053 79 12 3 83% 75 13 5 85%
355 P1361109 0 - O - 16 2 5 95%
356 P1361112A 0 - O - 33 4 3 100%
357 P13611128 39 27 1 100% 5 3 3 92%
358 P1361115 53 19 3 100% 12 8 4 100%
359 P1361116 73 28 2 100% 8 5 3 92%
360 P1391586 64 53 2 88% 203 57 3 100%

 

41

 

 

 

Replication] Replication2
Entry PU FC No of N0 of
number MAX MEAN ' DI MAX MEAN ° D1
plants plants
361 PI393541A 10 9 2 75% 36 11 3 92%
362 P1404168 24 O 3 83% 48 - 1 100%
363 P1404169A l7 l3 1 75% 1 16 90 2 100%
364 P14041698 O O 2 63% 20 14 2 88%
365 P140417] 0 - 0 - 71 64 3 92%
366 P1404175 12 7 3 67% 67 - 1 100%
367 P1404180 16 13 1 75% 139 105 3 92%
368 P1404182 0 - 0 - 1 18 - 1 75%
369 P1404186 O - 0 - 10 - 1 75%
370 P1404187 0 - 0 - - - 0 -
371 P1404188A 22 15 3 92% 10 8 3 100%
372 P14041888 O - 0 - 30 10 5 90%
373 P1404189 0 - 0 - 0 - 1 75%
374 P1404192A 65 53 1 75% 21 17 4 63%
375 P14041928 0 - 0 - 6 - l 75%
376 PI404192C O - 0 - - - 0 -
377 PI404196A 30 19 2 88% 47 34 3 100%
378 P14041968 0 - 0 - - - 0 -
379 P1405691 40 37 3 100% 52 41 2 100%
380 P14073868 56 39 2 100% 5 3 3 100%
381 P1407653 25 20 1 100% 27 16 5 100%
382 P1407654 23 3 3 83% 7 5 3 92%
383 P1407655A O 0 3 67% 2 1 4 81%
3 84 P14076558 28 25 2 75% 121 85 3 100%
3 85 PI407655C 25 l 3 3 83% 94 16 3 92%
386 P1407656 44 32 2 75% 10 1 2 88%
387 P1407657 0 0 3 58% 0 0 4 88%
388 P1407659A 3 1 3 58% 2 1 5 90%
389 P14076598 9 3 3 67% 0 0 3 100%
390 P1407722 44 13 2 100% 8 5 5 95%
391 P1407730 9 8 1 75% 20 15 2 75%
392 PI415073A 49 38 1 100% 33 26 2 88%
393 P1415074 0 - 0 - 16 12 4 75%
394 P1416823 93 13 3 83% 82 56 2 75%
395 P1416904A 19 12 3 83% 16 10 2 75%
396 P14169048 72 22 2 100% 87 50 3 75%
397 PI416904C 29 19 3 100% 8 3 2 63%
398 P1416936 13 10 1 100% 12 - 1 75%
399 P1417030 28 17 2 88% 56 34 5 75%
400 P1417040A 39 3O 3 100% 92 19 3 92%
401 P14170408 3 1 2 75% 0 - 1 100%
402 P1417059 1 5 9 2 100% 2 1 7 2 100%
403 P1417079 49 39 2 100% 90 5 l 5 90%
404 P1417094 47 22 5 95% 14 4 3 92%
405 P141724] 5 0 2 75% 13 . 4 5 75%

 

42

 

 

 

Replication] Replication2
Entry Pl] FC No of N0 01'
number MAX MEAN ' Dl MAX MEAN ' D1
plants plants

406 P1417242 51 27 2 100% 98 74 5 85%
407 P1417294 30 17 5 95% 133 119 2 88%
408 P1417295 50 27 3 92% 104 21 4 94%
409 P1417398 ‘ O - O - - - 0 -
410 P1430619 0 - 0 - 26 - 1 100%
41 1 P1430622 0 - 0 - 84 60 3 100%
412 P1430623 21 21 2 88% 70 - 1 75%
4 13 P1430624 14 6 2 75% - - 0 -
414 P1436562 O - O - - - 0 -
415 P143661] 0 - 0 - l3 8 3 100%
416 P1436684 13 1 l 1 100% 15 6 3 92%
417 P1437486 21 16 1 100% 67 39 4 100%
418 P1437487 32 29 2 88% 3 2 2 88%
419 P1437488 69 66 3 100% 1 1 8 2 100%
420 P1437554 32 23 2 100% 9 5 2 88%
421 P1437556 53 3 1 3 100% 5 1 10 4 94%
422 P1437558 28 20 3 92% 32 1 1 2 100%
423 P1437559 15 10 2 88% 71 52 3 100%
424 P1437563 0 - 0 - - - 0 -
425 P1437567 0 - 0 - 25 3 4 100%
426 P143757] 51 12 2 88% 8 5 3 100%
427 P1437573A O - 0 - 149 78 5 100%
428 P14375738 30 25 1 100% 32 14 5 95%
429 P1437574 48 35 1 100% 24 3 5 75%
430 P1437576 O - 0 - - - 0 -
431 P1437577 28 26 1 100% 16 6 5 85%
432 P1437578 O - O - 89 57 5 100%
433 P143758] 5 4 2 75% 0 0 4 88%
434 P1437584 0 - O - 23 14 5 100%
435 P1437585 1 1 0 2 88% 5 3 2 100%
436 P1437586A 50 3 2 88% 18 12 2 88%
437 P1437592 101 92 2 100% 33 21 3 100%
438 P14375938 64 42 2 100% 83 25 2 100%
439 P1437594A 14 5 2 63% 10 8 5 95%
440 P14375948 8 4 2 75% 3 1 3 100%
441 P1437595 16 6 4 100% 1 12 26 4 100%
442 P14375988 0 - O - 1 6 1 3 83%
443 P143760] 13 8 3 100% 43 14 5 90%
444 P1437602 0 - 0 - - - 0 -
445 P1437604 8 0 3 92% 8 1 2 100%
446 P1437605A 14 1 2 88% 39 3 l 2 100%
447 P14376058 3 2 5 100% 8 6 5 95%
448 P1437605C 14 10 5 100% 1 8 1 l 2 100%
449 P1437606 13 8 4 94% 58 25 3 100%
450 P14376098 28 25 4 94% 90 70 2 100%

 

43

 

 

 

Replication] Replication2
Entry PI, FC N f N 1
number MAX MEAN °‘ ° D1 MAX MEAN °' ° In
plants plants

451 P14376108 14 1 1 2 100% 58 31 3 100%
452 P14376148 5 3 3 67% 123 96 2 100%
453 P1437617 7 6 2 63% 149 88 3 100%
454 P1437618 4 1 2 88% 54 17 2 100%
455 P14376228 23 15 2 100% 91 76 2 100%
456 P1437626 12 12 4 88% 104 77 3 92%
457 P1437627 25 15 4 100% 1 12 84 3 92%
458 P1437630D 45 41 1 100% 8 - 1 100%
459 P143763] 43 41 2 100% 58 36 4 100%
460 P14376358 43 8 3 100% 132 1 1 3 3 100%
461 PI437635C 15 1 1 l 100% 19 15 3 92%
462 P1437635D 37 3 3 1 100% 102 71 3 100%
463 P14376358 87 76 5 100% 48 33 4 100%
464 P1437639 23 9 2 88% 38 2 l 3 100%
465 P14376408 26 1 8 1 100% 124 45 3 92%
466 PI437641A 2 1 1 75% 30 - l 100%
467 P14376418 1 1 6 l 75% 92 62 5 95%
468 P14376438 43 39 1 100% 132 121 2 88%
469 P1437644 35 23 1 100% 95 - l 100%
470 P1437647 10 6 3 92% 59 27 3 92%
471 P14376488 77 2 2 100% 82 26 5 90%
472 PI437649A 0 0 2 25% - - 0 -
473 P14376498 85 84 3 100% - - 0 -
474 P14376518 59 10 3 100% 54 - l 100%
475 P1437652 0 - 0 - 72 16 3 92%
476 P1437653 0 - 0 - 21 16 3 92%
477 Williams88 68 54 3 100% 71 - 1 100%
478 P1437655 22 1 8 3 100% 67 - 0 -
479 P1437657 6 0 2 100% 23 7 3 100%
480 PI437661A 8 1 5 90% 12 - 1 100%
481 P14376618 24 10 2 100% 96 39 3 92%
482 P1437666 48 43 2 88% 27 19 3 92%
483 P1437669 64 53 2 100% 86 48 3 100%
484 P1437673 0 - 0 - 102 - 1 100%
485 P1437674 14 10 2 88% 100 62 3 92%
486 P1437675 42 25 3 83% 90 66 2 88%
487 P1437677 0 - 0 - 64 52 3 75%
488 PI437678A 217 187 2 88% 46 35 3 100%
489 P14376788 61 53 3 100% 138 101 3 83%
490 814376824 219 130 2 88% 70 - 1 100%
491 P14376828 0 - 0 - 70 - 1 100%
492 P1437684 0 - 0 - - - O -
493 PI437685A 91 55 1 100% 36 5 3 92%
494 P14376858 109 11 4 100% 119 103 4 94%
495 PI437685C 76 3O 4 100% 146 5 5 3 92%

 

44

 

 

 

Replicationl Replication2
Entry ”I FC N f N 1'
number MA MEAN 0' 0 D1 MAX MEAN °' ° DI
X Jlants plants

496 PI437685D 89 69 4 88% 88 17 3 75%
497 P1437686 45 29 4 81% 43 23 4 88%
498 P1437687 1 l 2 2 75% 32 19 3 83%
499 P1437689 1 l 1 76 3 100% 38 5 4 100%
500 P1437690 68 55 5 95% 6 0 3 100%
501 P1437692 134 84 4 100% 26 18 2 88%
502 P1437693 1 6 10 1 100% - - 0 -
503 P143 7695A 23 1 5 1 75% 5 - 1 1 00%
504 P14376958 1 1 O 25% - - - -
505 P1437697 45 32 3 92% 103 - 1 100%
506 P1437698 100 88 1 100% 0 - 1 100%
507 P1437699 55 27 2 88% 56 34 2 88%
508 P1437700 51 42 3 92% 68 47 3 92%
509 P1437704 l 0 2 100% 55 45 3 83%
510 P1437705 0 - 0 - - - O -

5 l 1 P1437706 0 - 0 - 76 - 1 100%
512 P1437707 4O 36 l 100% 163 133 l 100%
513 P1437709 0 0 3 67% 21 15 3 92%
514 P1437710 5 0 2 88% 137 111 3 100%
515 P143771 1A 7 4 1 75% 63 41 1 100%
516 P1437713 17 2 4 75% 179 127 2 100%
517 P1437714 2 l l 75% - - 0 -
518 P1437715 72 52 2 100% - - 0 -
519 PI437716A 49 2 5 100% 142 105 3 92%
520 P14377168 12 9 3 100% 33 22 4 100%
521 P1437718 56 O 3 92% 32 10 3 100%
522 P14377218 103 34 3 100% 1 12 65 2 100%
523 PI437721C 0 - 1 100% 212 39 2 100%
524 P1437723 5 O 3 100% 81 52 3 100%
525 P1437724 48 45 3 67% 23 20 3 100%
526 P1437729 O - 0 - - - O -
527 P1437733 0 - 0 - - - 0 -
528 P1437737 l 1 1 75% - - 0 -
529 P1437738A 25 7 2 88% 25 21 1 100%
530 P1437739 0 - 0 - 59 40 1 75%
53] P143774] 17 9 4 88% - - 0 -
532 81437743 0 0 1 50% 92 42 2 100%
533 P1437752A 0 - O - 71 41 3 25%
534 P14377528 57 46 4 88% 1 l 3 5 100%
535 P1437752C 56 48 l 100% 71 36 3 92%
536 P14377538 64 55 2 75% 41 33 2 100%
537 P1437765 19 13 3 83% 37 27 3 92%
538 P1437770 55 5 3 100% 53 33 5 95%
539 P1437776 13 10 l 100% 52 48 3 100%
540 P1437777 84 64 3 100% 52 3 5 3 1 00%

 

45

 

 

 

Replicationl Replication2
Entry PU FC N f N 1'
number MAX MEAN °' ° D1 MAX MEAN °' ° DI
plants plants

54] P1437787 1 10 93 4 100% 0 O 3 92%
542 PI437788A 66 37 3 100% 29 3 3 100%
543 P143779] 63 51 3 92% 60 17 3 100%
544 P1437792 62 58 3 92% 91 12 3 100%
545 P1437793 80 58 4 100% 70 47 3 92%
546 P1437794 65 38 2 88% 67 43 3 75%
547 P1437795 153 80 3 100% 56 38 4 94%
548 P1437797 0 - 0 - 141 14 3 100%
549 P1437802 0 - 0 - - - 0 -

5 50 P1437803 74 64 4 100% 52 42 4 94%
551 P1437804 1 1 8 2 100% 34 28 1 100%
552 P1437805 44 33 3 92% 74 33 3 100%
553 P1437806 169 103 3 100% 158 31 3 100%
554 P1437810 83 71 3 100% 67 12 5 90%
555 P143781] 0 - 0 - 99 4O 4 100%
556 P1437834A 75 63 l 100% 71 33 3 83%
557 P14378348 70 38 1 100% 57 38 3 75%
558 P1437836 126 1 1 1 4 100% 27 15 3 100%
559 PI437839A 0 - O - 273 1 80 3 100%
560 P14378398 1 14 85 4 100% 56 42 4 94%
561 PI437840A 107 73 3 92% 153 64 3 100%
562 P14378408 88 62 3 92% 80 53 2 88%
563 P143784] 151 92 2 100% 61 17 4 94%
564 P1437842 0 - O - - - 3 92%
565 PI437843A 0 0 l 75% 90 43 0 -
566 P14378438 54 39 4 94% 1 13 32 2 88%
567 P1437844A 75 4 1 2 100% - - 0 -
568 P14378448 34 26 1 100% 60 35 5 95%
569 P1437845A 2 l O - 74 47 3 83%
570 P14378458 67 52 5 100% 163 1 15 4 88%
571 PI437845C 43 37 1 100% 58 34 5 95%
572 PI437848A 23 7 4 88% 23 l 1 4 100%
573 P14378488 46 2 5 100% 12 5 2 100%
574 P1437850 93 66 4 100% 42 25 4 94%
575 P14378518 55 49 1 100% 22 9 3 100%
576 P14378538 83 68 3 100% 12 1 27 4 100%
577 PI437856A 156 89 3 100% 30 21 3 100%
578 P14378568 70 66 2 100% 38 29 5 95%
579 PI437857C 45 35 5 100% 190 120 4 88%
580 P14378608 139 138 3 100% - - 1 100%
58 l P1437863A 45 3 3 4 100% - - 1 75%
582 P14378638 1 17 61 2 100% 47 34 2 75%
583 P14378648 127 11 1 4 100% 42 38 3 75%
584 P1437865 0 - 4 100% 24 12 3 92%
585 PI437867A 34 29 O - 46 38 5 95%

 

46

 

 

 

Replicationl Replication2
Entry PU FC N f N 1'
number MAX MEAN °' ° D1 MAX MEAN °' ° D1
plants plants

5 86 P14378678 80 41 1 100% 104 72 3 92%
587 P1437870 0 - 0 - - - 0 -

5 88 P143787] 33 26 1 100% 74 54 4 88%
589 P1437873 74 68 1 100% 100 - 3 83%
590 P1437874 69 4 2 100% - - 3 92%
591 P1437875 89 78 2 100% - - 0 -
592 P1437876 88 32 3 100% - - 0 -
593 P14378778 0 - 4 100% 1 5 10 4 94%
594 PI437877C 168 124 0 - 7 5 5 95%
595 P14378788 0 - 2 100% 20 5 3 92%
596 P1437879 0 - 0 - - - 0 -
597 P1437880 1 13 81 0 - 74 59 4 100%
598 P143788] 168 142 3 100% 70 12 3 92%
599 P1437882A 137 66 2 100% 7 - 1 75%
600 P14378828 0 - 2 100% - - O -
601 P1437883 103 30 2 100% 17 2 2 100%
602 P1437884 25 18 1 100% - - 1 100%
603 P1437885 62 45 2 100% - - O - '
604 P14378868 1 18 22 1 75% 205 134 2 75%
605 P14378878 104 86 1 100% 127 76 2 100%
606 PI437887C 125 92 1 100% 198 147 5 95%
607 P14378888 0 - 0 - - - O -
608 P1437889 103 70 1 75% 12 5 3 100%
609 P14378908 12 6 4 94% 28 12 4 94%
610 P143789] 89 71 1 100% 3 l 5 100%
611 P1437893 34 28 4 100% 9 4 3 92%
612 P1437894 38 14 3 100% 9 4 3 83%
613 P1437896 7 5 2 75% 6 3 5 95%
614 P1437897 102 32 3 100% 12 8 4 94%
615 P1437898 150 122 3 100% 1 1 6 4 94%
616 P1437899 103 89 3 100% 9 6 5 95%
617 P1437900 0 - 0 - 10 - 1 100%
618 PI437902A 98 78 4 100% 14 5 3 100%
619 P14379028 104 87 2 88% 42 28 3 100%
620 P1437902C 76 63 2 100% 3 1 3 3 100%
621 P1437902D 21 12 ' 1 75% 6 4 3 92%
622 P1437903 103 82 4 94% 36 25 4 94%
623 P1437904 33 26 2 100% 93 56 O -
624 P1437907 41 9 3 100% 5 3 O -
625 P1437908 34 34 1 100% 62 48 5 90%
626 PI437909A 45 9 4 100% 30 22 5 90%
627 P14379098 21 20 3 100% 1 13 79 3 92%
628 PI437910C 22 18 5 100% 122 108 3 92%
629 P143791] 10 8 1 100% 42 - 1 100%
630 P1437912 48 39 1 100% 77 61 5 90%

 

47

 

 

 

Replicationl Replication2
Entry PU FC N f N 1'
number MAX MEAN °' ° D1 MAX MEAN °' ° Dr
plants plants

631 P1437914 17 12 2 100% 91 60 5 95%
632 P1437918 31 21 2 100% 65 53 4 88%
633 P1437919 33 30 1 100% 88 73 4 94%
634 P1437920C 12 1 1 4 88% 0 0 3 92%
635 P143792] 0 - O - - - 0 -
636 P1437922 52 17 2 100% 82 59 4 94%
637 P1437926 9 7 2 88% 11 1 20 3 100%
63 8 P143793] 28 24 3 100% 47 32 4 94%
639 P1437932 24 17 1 100% 74 ' 1 1 4 88%
640 P1437935 37 30 5 95% 101 81 4 100%
641 P1437940 46 23 3 100% 42 30 5 95%
642 P1437943 32 28 4 100% 53 45 3 100%
643 P1437946A 5 2 3 83% 46 40 5 95%
644 P14379468 0 - 0 - 32 20 3 92%
645 P1437950 6 2 4 88% 12 9 2 88%
646 P1437954 59 39 0 - 23 1 3 5 100%
647 P14379568 19 4 2 100% 1 15 88 5 100%
648 P14379578 0 - 0 - 82 72 4 94%
649 P143796] 80 22 2 100% 64 38 4 94%
650 PI437964A 38 4 3 100% 72 54 4 100%
65 1 P14379648 1 7 14 2 100% 1 1 5 42 3 67%
652 P1437970 5 3 1 100% 92 39 2 63%
653 P1437973 263 243 2 100% 26 21 2 88%
654 P14379748 15 1 50 3 100% 33 3 2 100%
655 PI437985A 126 121 2 100% 31 18 4 88%
656 P14379858 37 34 2 100% 9 5 4 100%
657 P1438001 32 29 3 92% 74 55 5 95%
658 P1438003 45 4O 1 100% 57 47 4 100%
659 P143801] 28 18 1 100% 47 25 3 100%
660 P14380198 32 10 4 100% 43 32 4 100%
661 P1438046 31 14 3 100% - - O -
662 P1438047 27 0 3 92% l l 1 31 5 100%
663 P143805] 8 109 103 3 100% 121 81 5 95%
664 P1438057 17 3 4 100% 18 1 5 5 4 88%
665 P143806] 1 10 105 3 100% 90 27 4 94%
666 P1438066 10 1 3 92% 8 l 5 95%
667 P1438069A 92 39 3 100% 78 61 5 100%
668 P14380698 2 1 1 75% 13 10 5 90%
669 PI438069C l 12 104 2 8 8% 30 12 3 92%
670 P1438070 0 - O - 20 13 2 100%
671 P1438075 76 72 3 92% 11 1 36 2 75%
672 P1438076 29 23 1 75% 12 7 3 100%
673 P1438079 16 6 2 88% 85 41 3 100%
674 P1438080 36 12 2 100% 5 1 5 95%
675 P1438083 65 54 1 100% . 52 27 5 95%

 

48

 

 

 

Replicationl Replication2
Entry PU FC N r N 1
number MAX MEAN °' ° DI MAX MEAN °' ° 91
plants plants

676 P1438084 186 l l 1 3 92% 48 39 3 100%
677 P1438085 70 30 3 92% 105 13 3 100%
678 P1438086 1 1 1 74 2 100% 1 19 68 2 100%
679 P1438098 0 - 0 - - - 0 -
680 P1438098 49 36 3 100% 155 83 2 100%
681 P143810] 8 0 2 88% 247 156 5 90%
682 P1438102 95 88 3 92% 378 134 5 95%
683 P1438103 57 54 3 92% 277 145 5 95%
684 P1438104 42 30 3 100% 198 1 14 5 95%
685 P14381058 41 38 4 100% 18 11 3 83%
686 P1438109C 101 21 3 92% 86 13 5 95%
687 P14381128 40 2 2 88% 0 0 4 94%
688 P1438114 39 18 3 75% 226 187 5 100%
689 P14381248 6 4 1 100% 506 232 4 94%
690 P1438129 27 23 3 83% 43 32 5 90%
691 P1438130 71 65 1 100% 173 19 5 100%
692 P1438131 O - 0 - - - 0 -
693 P1438132 6 4 4 75% 83 42 4 100%
694 P1438133A 10 7 2 75% - - 0 -
695 P14381338 O 0 1 75% 49 21 4 88%
696 P1438139 18 4 3 92% 130 68 4 81%
697 P1438144 37 3 3 75% 19 10 2 75%
698 P1438153 53 46 4 88% 18 9 3 100%
699 P1438163 29 22 3 83% 68 6 5 95%
700 P14381648 82 32 4 100% 1 10 56 4 100%
701 P1438167 27 15 3 100% 151 94 5 100%
702 P1438168 33 21 2 88% 77 17 3 100%
703 P1438173 16 12 2 100% 32 8 2 100%
704 P1438175 51 25 3 92% 77 20 4 100%
705 P1438176 0 - 0 - 57 36 5 100%
706 P1438177 22 6 3 83% 93 8O 3 92%
707 P1438178 44 28 1 100% 149 108 3 92%
708 P1438183 7 3 2 88% 69 45 3 100%
709 P1438185 0 0 3 92% 22 1 1 5 85%
710 P1438186 15 6 3 83% 2 l 4 100%
71 1 P1438192 15 13 3 100% 160 93 5 100%
712 P1438194 14 13 1 100% 116 93 3 100%
713 P1438197 22 14 1 100% 310 148 2 88%
714 P1438198 44 31 3 92% 184 41 4 94%
715 P1438199 0 - O - 106 ll 4 75%
716 P1438212C 61 57 l 100% 163 135 5 95%
717 P1438214 39 5 2 100% 24 9 2 100%
718 P1438216 0 - O - 37 27 5 100%
719 P1438219 0 - 0 - 55 - 1 100%
720 P1438222 17 14 3 92% 60 43 3 92%

 

49

 

 

 

Replication] Replication2
Entry PU FC N r N 1
number MAX MEAN °' ° DI MAX MEAN °' ° D1
plants plants

721 P1438223 103 18 4 100% 145 40 3 100%
722 P1438226 36 31 3 100% 176 1 16 3 92%
723 P1438227 96 31 2 100% 280 192 3 100%
724 P1438228 44 27 2 100% 295 44 4 94%
725 P1438229 o - 0 — - - 0 -

726 P14382348 58 46 3 100% 179 1 17 4 94%
727 P1438235 55 41 4 100% 374 285 4 94%
728 P1438236 21 17 2 88% 84 25 2 88%
729 P1438237 0 - 3 100% 47 33 4 94%
730 P1438242 0 - 0 - 15 - 1 100%
731 P1438244 176 94 0 - 83 69 4 100%
732 P1438249A 166 85 3 75% 107 90 4 94%
733 P14382498 0 0 2 100% 10 4 4 88%
734 P14382508 3 2 3 92% 91 30 3 100%
735 P1438251 17 12 5 100% 130 1 16 5 90%
736 P14382528 1 1 8 1 100% 146 126 3 83%
737 PI438252C 48 45 2 100% 8 6 3 100%
738 PI438254D 41 32 3 92% 35 24 5 95%
739 P14382568 24 21 1 100% 16 10 3 100%
740 P1438257A 56 35 3 100% - - 0 -

741 P14382578 141 135 3 100% 43 23 3 100%
742 P1438258 72 52 2 88% 17 - 1 100%
743 P14382598 80 68 3 100% 99 - 1 100%
744 P1438261 91 61 3 100% 151 96 4 100%
745 P1438262 52 40 3 100% 101 - 1 75%
746 P1438263 31 21 2 100% 57 47 3 100%
747 PI438266A 27 20 3 75% - - 0 -

748 P14382668 50 40 2 88% 72 - 1 100%
749 P1438270 72 62 3 100% 33 19 4 100%
750 P14382718 101 97 3 92% 7 4 2 100%
751 P1442004 19 15 1 100% - - o -

752 P1458510 129 29 3 100% 85 - 1 100%
753 P14585198 79 73 3 100% 55 21 3 100%
754 P1458521 78 27 3 100% 24 15 4 100%
755 P14588258 0 - 0 - - - 0 -

756 P14588268 67 33 4 100% 55 33 5 90%
757 P1458828 1 1 1 3 83% 12 8 5 100%
758 P1464877 17 15 3 75% 41 31 5 95%
759 P1464879 61 42 3 100% 80 45 4 100%
760 P1464902 26 22 1 100% 36 - 1 75%
761 P1464910 41 11 4 94% 1 16 - 1 100%
762 P14649148 64 24 3 100% 8 2 5 100%
763 P1464916 90 45 2 88% 61 16 3 92%
764 PI464920A 0 - 0 - — - 0 -

765 P14649208 80 56 3 92% 6 4 4 94%

 

50

 

 

 

Replication] Replication2
Entry PI, FC No of No of
number MAX MEAN ' DI MAX MEAN ' DI
plants plants

766 P1464923 0 - 0 - 28 15 3 100%
767 P1464924 70 46 2 88% 73 34 4 94%
768 P1467326 30 24 1 75% 27 1 1 5 90%
769 P1468384 0 - 0 - - - 0 -
770 P1468385 71 43 3 83% 37 - 1 100%
771 P1468914 83 35 4 88% 89 - 1 75%
772 P1468919 3 2 0 - - - O -
773 P14702278 62 55 4 100% 56 29 4 100%
774 P1475810 121 1 10 1 100% 57 26 3 92%
775 P147581 1A 33 28 4 94% 9 4 3 100%
776 P14758118 4O 16 3 100% 27 20 3 100%
777 P1475812A 20 12 4 100% 96 76 2 100%
778 P14758128 91 68 3 100% 12 5 5 100%
779 P1475813A 6 3 5 95% l 12 - l 100%
780 P14758138 3O 6 4 88% 36 26 4 100%
781 P1475814 45 32 3 92% 19 13 2 100%
782 P1475815 9 8 3 75% 64 44 4 100%
783 P1475817 4 2 2 75% 160 133 4 100%
784 P1475818 8 3 3 58% 74 53 2 100%
785 P1475819 91 33 3 92% 107 28 2 100%
786 P1475820 99 92 2 100% 281 79 3 100%
787 PI475822A 10 2 1 75% 107 66 2 100%
788 P14758228 34 0 3 83% 42 32 3 100%
789 PI475822C 9 7 4 75% 13 10 2 100%
790 P1475823 5 0 3 67% 256 169 2 100%
791 P1475824A 5 8 44 3 58% l 17 92 3 100%
792 P14758248 9 4 3 58% 1 89 49 4 100%
793 P1475826 39 31 3 75% 143 101 3 92%
794 P1475828 O - 3 67% 281 137 2 100%
795 P14758298 13 10 0 - 9 - 1 100%
796 P1475830 l 12 84 2 50% 59 44 5 75%
797 P147583] 14 7 3 92% 46 16 3 100%
798 P1479720 15 10 4 100% 52 31 2 100%
799 P1479722 14 10 2 63% 38 21 2 100%
800 P1479729 17 14 3 92% 42 35 2 100%
801 P1479730 0 - 0 - 64 49 2 88%
802 P1479735 19 15 2 50% 36 25 4 75%
803 P1479740 25 5 2 63% 46 32 5 95%
804 P147974] 6 2 4 88% 23 5 5 100%
805 P1490765 96 l 5 2 100% 7 2 2 100%
806 P1490766 128 1 15 3 92% 1 12 41 2 88%
807 P1490767 96 73 2 100% 13 2 3 83%
808 P1490768 0 - 2 75% 21 8 2 88%
809 P1490769 12 10 3 100% 11 8 2 88%
810 P1495017A 7 4 1 100% - - 0 -

 

51

 

 

 

Replication] Replication2
Entry PU FC N f N 1’
number MAX MEAN °' ° DI MAX MEAN °' ° DI
plants Jlants

81 1 P1503334 89 22 1 75% - - 0 -
812 P1507543 O 0 100% - - 0 -
813 P1518715 30 8 l 75% 25 - 1 100%
814 P1518716 10 5 l 75% 36 - l 100%
815 P1532442 3 1 2 100% 133 4 1 3 100%
816 P1532448 5 8 9 3 100% 13 10 3 92%
817 PIS32462A 1 19 104 2 88% 99 21 3 100%
818 P1532463A 107 62 2 100% 102 54 3 100%
819 P15324638 167 147 5 100% 20 3 2 88%
820 P1538375 17 2 2 100% 2 - 1 75%
821 P1538376 149 105 3 100% 40 - 1 75%
822 P1538377 56 45 4 100% 22 14 3 100%
823 P1538380 99 61 3 100% 63 29 2 88%
824 P153838] 161 109 5 95% 34 22 2 88%
825 P1538382 5 3 2 75% 45 5 5 90%
826 P1538386A 59 42 2 100% 45 26 3 100%
827 P1538397 76 24 2 100% 169 67 3 100%
828 P1538398 41 25 2 100% 87 62 5 95%
829 P1540739 5 4 1 100% 37 28 4 100%
830 P1549021A 31 4 4 100% 52 41 3 92%
831 P1549029 9 5 4 100% 103 94 0 -
832 P1549030A 5 4 0 - 23 - 1 100%
833 P154903] 65 40 2 100% 101 78 3 100%
834 P1549041A 0 O 1 100% 21 l 1 5 95%
835 P1556948 27 14 3 100% 50 36 4 94%
836 P1561227 0 - 0 - 186 - 1 75%
837 P1561228 0 - 0 - 77 - 1 75%
838 P1561229 0 - O - - - 0 -
839 P1561231 0 - 0 - 34 - 1 100%
840 P1561232 0 - 1 100% 25 8 5 95%
841 P1561233A 41 31 0 - 59 34 3 92%
842 P1561234 0 - 0 - 9 - 1 100%
843 P1561235 0 - 0 - - - 0 -
844 P1561 236 0 - 0 - 1 2 - 1 75%
845 P1561238 37 34 1 100% 55 21 4 88%
846 P156] 24] 0 0 1 100% 16 5 5 95%
847 P1561295 73 67 1 100% - - 0 -
848 P1561296C 31 20 1 100% 5 - 1 75%
849 P1561297 48 25 2 100% 1 1 - 1 100%
850 P1561298 37 31 1 100% 7 5 2 88%
851 P15612998 27 21 1 100% - - 0 -
852 P1561300A 41 15 3 100% 17 13 3 100%
853 P15613008 0 - 3 100% 9 7 3 1 00%
854 P1561301 64 52 1 100% - - 0 -
855 P1561302C 27 20 1 75% 1 - 1 100%

 

52

 

 

 

Replicationl Replication2
Entry PU FC N f N 1‘
number MAX MEAN °' ° DI MAX MEAN °‘ ° 81
plants plants

856 P15613028 0 - 0 - 32 20 3 92%
857 P1561304A 0 - 0 - 12 7 3 100%
858 P15613048 1 13 98 4 100% - - 0 -
859 P1561308 69 48 3 100% 1 1 5 l 75%
860 P1561309A 47 36 2 88% 4 - 1 75%
861 P15613098 l 14 2 2 100% 93 - 1 75%
862 P1561310 102 8 5 100% 61 - 1 75%
863 P1561311A 59 37 3 92% 152 43 4 100%
864 P15613118 74 30 2 88% - - 0 -
865 P1561312 24 10 4 88% 137 104 3 83%
866 P1561314A 0 - 0 - 14 11 3 100%
867 P15613148 0 - 0 - 52 40 3 100%
868 P1561315 63 33 3 92% 81 65 3 100%
869 P1561316 3 2 1 100% 1 - 1 100%
870 P1561317 0 - 0 - 132 83 5 90%
871 P1561318A 107 94 3 100% 42 23 2 75%
872 P15613188 101 91 2 100% 20 16 2 75%
873 P1561319A O - 0 - 84 40 4 81%
874 P15613198 0 - 0 - 36 - 3 75%
875 P1561320 0 - 1 75% 65 31 5 95%
876 P156132] 37 21 3 92% 13 3 4 88%
877 P1561322 73 14 2 100% 105 36 4 94%
878 P1561323 7 2 1 100% 113 29 3 83%
879 P1561324 0 - O - 7 - 3 100%
880 P1561328 14 6 4 94% 44 18 3 100%
881 P1561329 0 - 0 - - - O -
882 P1561330A 76 43 2 100% 125 - 1 100%
883 P15613308 0 - 0 - 36 1 2 5 90%
884 P156133] 34 31 3 83% 115 87 4 88%
885 P1561333 O - 0 - 44 26 4 94%
886 P1561335 92 85 2 100% 101 84 2 100%
887 P1561336 O - 0 - 180 49 2 88%
888 P1561337 103 22 1 100% 95 74 4 88%
889 P1561338A 29 18 3 100% 105 87 2 100%
890 P15613388 34 15 2 100% 73 55 2 100%
891 P1561339 7 3 1 100% 27 - 1 100%
892 P1561340 15 10 3 92% 1 15 45 4 94%
893 P1561341A 0 - 0 - 83 24 4 100%
894 P15613418 0 - 0 - l7 9 2 100%
895 P1561345 67 55 1 100% 280 189 3 100%
896 P1561346 65 54 3 100% 152 - l 100%
897 P1561347 1 14 39 1 100% 126 19 2 88%
898 P1561348 O - 0 - 52 - 1 100%
899 P1561349 44 1 7 2 100% 7 l 60 3 100%
900 P1561350A 27 22 2 100% 26 13 3 100%

 

53

 

 

 

Replication] Replication2
Entry P1/ FC N f N 1’
number MAX MEAN °' ° DI MAX MEAN °° ° DI
plants plants

90] P15613508 O - 0 - 28 16 3 100%
902 P1567174C 0 - 0 - l 1 5 89 3 100%
903 P1567284 O - O - 57 43 5 95%
904 P15672888 81 67 1 100% 143 - 1 100%
905 P1567296A 13 9 3 92% 96 - 1 75%
906 P1567302 39 3 l 3 100% 1 74 l 16 3 100%
907 P15673518 107 10 2 75% - - 2 88%
908 P1567482A 109 0 2 88% 45 17 5 100%
909 P15674828 0 - 0 - 77 73 2 100%
910 P1567482C 0 - 0 - 73 - l 100%
91 1 P1567484 16 O 3 92% 67 4O ' 2 88%
912 P1567487 23 21 1 100% 155 85 2 88%
913 PIS67489A 45 24 5 90% 6 - l 100%
914 P15674898 2 l 1 8 2 63% 65 43 4 100%
915 P1548445 157 132 2 25% 27 17 3 25%
916 P1567493 57 50 3 58% 13 - 4 88%
917 P1567494 3 8 32 2 63% 47 27 4 100%
918 P1567496 20 2 3 67% 17 12 4 88%
919 P1567497 34 1 8 4 88% 6 - l 100%
920 P1567498 45 26 3 92% 1 1 7 24 4 94%
921 P1567499 40 23 4 75% 7 4 4 94%
922 P1567500 16 9 1 75% 7 4 3 92%
923 P1567502 0 - 0 - 5 4 5 95%
924 P1567506 1 10 92 2 100% 1 19 103 3 100%
925 PIS67507C 1 l l 50 1 100% 2 0 3 92%
926 PIS67508A 109 5 1 4 69% - - 0 -
927 P1567510A 76 16 2 100% 1 1 - 1 100%
928 P15675108 77 47 1 100% - - 0 -
929 P156751] 14 7 l 100% 10 5 3 100%
930 P1567512C 78 62 4 88% 7 5 4 75%
931 P1567513 63 33 4 88% 11 5 3 83%
932 P15744808 10 3 1 75% 1 17 48 3 75%
933 P1578360 22 14 4 100% 272 1 12 2 100%
934 P1578362 1 5 0 3 92% 10 6 5 100%
935 P1578363 160 139 2 88% l 13 65 5 75%
936 P1578364 28 14 1 100% 86 71 5 80%
937 P1578365 310 162 1 100% 67 33 4 81%
938 P1578366 17 9 3 92% 42 35 5 100%
939 P1578367 0 - O - 91 68 3 100%
940 P1578368 42 27 1 100% 1 70 - 1 100%
941 P1578369 83 71 3 100% 54 - 1 75%
942 P1578370 0 - 0 - 0 0 3 83%
943 P1578374 49 - 4 1 00% O - 1 75%
944 P15783758 3 l - 3 1 00% 59 44 3 100%
945 P1578376 95 - 3 100% 102 63 3 83%

 

54

 

 

 

Replicationl Replication2
Entry PU FC N r N 1
number MAX MEAN °° ° DI MAX MEAN °' ° D1
Jlants plants

946 P1578378 42 - 3 100% 21 9 3 83%
947 P1578380A 0 - 0 - 186 134 2 100%
948 P15783808 0 - 1 100% 57 30 3 92%
949 P1578382 10 - 2 100% 38 22 5 100%
950 P1578384 15 - 2 100% 21 15 4 94%
951 P1578385 0 - 1 100% 21 13 3 92%
952 P15783888 0 — 0 - 39 3o 2 100%
953 P1578390 44 - 2 100% 31 21 5 95%
954 P1578392A 17 - 3 100% - - 0 -

955 P15783928 7 - 3 100% 101 - 1 100%
956 P1578399 0 - 0 - 3 1 3 100%
957 P1578400 52 46 1 100% 139 68 5 100%
958 P1578401A 5 3 1 100% 91 21 3 75%
959 P15784018 27 17 1 100% 12 - 1 75%
960 P1578401C 6 4 1 100% 93 - 1 75%
961 P1578401D 15 0 2 100% 83 17 5 50%
962 P1578402 0 - 1 100% 29 - 1 100%
963 P1578403 0 - 1 75% 35 - 1 75%
964 P1578405 0 - 1 100% 1 19 - 2 63%
965 P1578406 0 — 1 75% - - 1 75%
966 P1578407 0 - 1 75% 89 - 1 100%
967 P1578408 0 - 0 - 13 2 5 80%
968 P1578409A 33 - 2 88% 6 - 1 100%
969 P15784098 0 - 1 100% 77 67 3 100%
970 P1578410 0 - 1 100% 7 — 1 75%
971 P1578411 0 - 0 - 33 21 5 95%
972 P1578412 10 - 3 58% 6 4 3 100%
973 P1578413 0 - 0 - - - 0 -

974 P1578414 0 - 1 75% 1 16 24 3 100%
975 P1578416 0 — o - - - 0 -

976 P15784178 28 - 2 100% 93 80 2 100%
977 P1578418 44 — 2 75% 77 - 1 100%
978 P1578419A 27 - 4 69% 80 - 1 100%
979 P15784198 29 - 3 75% 85 - 1 100%
980 P1578420 0 - 0 - 5 3 4 100%
981 P157842] 34 - 2 100% 35 12 5 100%
982 P1578425 45 - 1 100% 18 2 4 100%
983 P1578431 38 - 3 92% 38 32 3 92%
984 P15784328 0 - 0 - 0 - 1 100%
985 P1578473A 6 - 3 100% 81 32 3 100%
986 P157848] 12 - 2 88% 21 15 3 92%
987 P1578497A 19 - 3 100% 33 15 3 92%
988 P15784978 18 - 5 85% 39 20 3 100%
989 P1578499A 5 - 3 100% 107 83 3 100%
990 P15784998 10 - 3 100% 109 89 2 100%

 

55

 

 

 

Replicationl Replication2
Entry "I FC N r N 1
number MAX MEAN °‘ ° DI MAX MEAN °° ° DI
plants plants

991 P1578499C 65 - 4 100% 162 57 3 92%
992 P1592899 0 - 0 - 1 10 10 4 100%
993 P1592901 54 — 3 83% 80 8 4 100%
994 P1592927 44 - 5 75% 72 25 3 100%
995 P1592928 56 - 1 100% 191 106 3 83%
996 P1592945 36 - 3 100% 134 60 3 100%
997 P1592956A 30 - 3 100% 62 4 4 100%
998 P15929568 22 - 4 100% 371 47 3 92%
999 P1592956C 1 - 3 100% 1 10 14 2 100%
1000 P1592971 26 — 3 100% 87 80 3 75%
1001 P1592974 33 - 3 100% 126 26 3 100%
1002 P1592978 6 - 3 92% 51 37 5 70%
1003 P1592980 41 - 3 100% 1 12 16 5 90%
1004 P1592981 31 - 3 100% 38 33 5 100%
1005 P1593949A 17 - 4 100% 41 24 3 92%
1006 P15939498 20 - 4 100% 101 70 2 100%
1007 P1593956A 24 - 4 88% 165 33 3 75%
1008 P15939568 0 - 0 — 58 10 3 100%
1009 P1593956C 1 1 - 5 95% 25 16 4 100%
1010 P1593956D 16 - 1 100% 60 49 3 75%
101 1 P15939568 37 - 2 100% 125 89 3 92%
1012 P1593957 35 - 3 100% 33 17 4 94%
1013 P1593961 67 - 2 100% 4 1 3 75%
1014 P1593962 0 - 0 — 53 46 2 100%
1015 P1593963 42 31 4 100% 33 27 3 100%
1016 P1593964 36 32 2 88% 40 28 2 88%
1017 P1593966 61 2 3 83% 11 9 2 100%
1018 P1593967 12 6 2 100% 38 6 5 100%
1019 P1593968 53 30 4 100% 150 91 2 100%
1020 P1593969 0 - 0 - - - 0 -

1021 P1594899A 89 5 4 100% 175 1 16 4 100%
1022 P15948998 68 53 2 100% 1 15 81 3 100%
1023 P1594900A 71 57 3 100% 8 1 5 95%
1024 P1594902 98 63 4 100% 1 10 24 3 75%
1025 P1594909 36 14 4 75% 39 23 3 100%
1026 P1602491 101 79 4 81% 61 50 3 92%
1027 P1602492 33 4 2 63% 73 12 3 100%
1028 PI602497A 80 40 2 50% 10 7 2 100%
1029 P1602498 65 14 1 75% 214 142 4 100%
1030 P1602499 98 12 2 25% - - 0 -

1031 PI602502A 36 25 2 50% 3o - 1 75%
1032 P1603290 97 3 2 100% 36 9 2 100%
1033 P1603366 89 45 3 75% 71 10 2 100%
1034 P1603384 51 33 2 63% 17 4 3 100%
1035 P1603388 45 31 4 69% 25 7 3 100%

 

56

 

 

 

Replication] Replication2
Entry PI] FC N r N 1
number MAX MEAN °' ° D1 MAX MEAN °‘ ° D1
plants plants

1036 P1603392 14 7 4 25% 12 5 4 25%
1037 P1603393 26 17 3 50% 129 22 3 100%
1038 P1603394 39 34 2 75% 27 14 5 100%
1039 P1603395 3O 22 4 69% 19 13 2 88%
1040 P1603396 20 12 2 75% 18 1 4 75%
1041 P16033988 4 l 2 100% 4 3 4 100%
1042 P1603400 0 - 0 - 69 43 3 83%
1043 P1603402 3 8 32 l 100% 104 64 3 100%
1044 P1603406 l 6 6 2 88% 1 5 8 2 100%
1045 P1603408 82 9 2 100% 30 - 1 100%
1046 P1603415 36 16 2 100% 91 63 2 100%
1047 PI603418A 90 67 1 100% 1 14 86 4 94%
1048 P16034188 40 10 2 50% 37 15 4 100%
1049 P1603418C 16 8 3 25% 63 42 3 25%
1050 PI603418D 27 17 3 75% 135 77 3 92%
1051 P16034218 45 4 2 100% 76 45 3 100%
1052 P16034228 21 0 1 100% 51 34 3 100%
1053 PI603423A 157 81 4 75% 42 26 2 100%
1054 P16034238 57 1 3 100% 100 77 3 92%
1055 P16034248 3 8 0 2 100% 104 79 3 100%
1056 PI603424C 4 0 3 100% 36 27 4 75%
1057 PI603424D 21 1 3 75% 129 48 4 100%
1058 P1603426F 36 0 3 92% 193 159 3 100%
1059 PI603427A 31 5 2 88% 161 95 2 88%
1060 P16034278 25 l 5 4 88% 32 1 3 2 100%
1061 PI603427C 33 29 4 94% 159 120 5 100%
1062 P1603428A 16 14 1 75% 15 2 3 92%
1063 PI603428C 82 60 3 75% 346 70 2 88%
1064 PI603428D 24 10 1 75% 22 2 4 88%
1065 PI603429C 87 48 3 100% 27 l 8 4 94%
1066 P16034308 56 37 1 75% 1 89 149 4 100%
1067 P160343] 92 86 4 100% 67 - l 100%
1068 PI603432C 0 0 3 92% 96 59 3 100%
1069 PI603438A 2 1 l 1 3 100% 0 0 4 50%
1070 P16034388 9O 73 1 100% 5 O 3 100%
1071 P16034388 14 8 3 92% 107 91 2 100%
1072 P1603439 13 9 2 100% 4 1 3 92%
1073 P16034408 83 41 2 88% 21 - 1 75%
1074 PI603440C 113 85 1 100% 0 0 4 88%
1075 P1603442 104 80 2 100% 255 150 3 92%
1076 P1603443A 1 12 21 3 92% 13 6 2 100%
1077 P16034438 16 0 3 83% 1 15 1 1 2 100%
1078 P16034448 46 13 3 75% 51 48 4 94%
1079 P1548657 5 8 54 1 50% - - 0 -

1080 PI603445A 79 63 l 100% 227 - 1 100%

 

57

 

 

 

Replicationl Replication2
Entry PU FC N f N 1'
number MAX MEAN °° ° DI MAX MEAN °‘ ° DI
plants plants

1081 P1603447 76 53 1 100% 186 32 2 100%
1082 P1612729 76 67 3 92% 26 20 3 83%
1083 P1612730 65 59 4 50% - - 0 -
1084 P161273] 54 51 3 75% 17 13 3 100%
1085 P1612732 81 65 2 88% 37 31 2 100%
1086 P1612733 78 74 3 100% 42 3O 3 100%
1087 P1612734 78 41 4 88% 8 5 2 100%
1088 P1612735 10 6 2 100% 13 9 3 100%
1089 P1612736 49 25 3 100% 61 48 4 94%
1090 P1612737 99 86 2 100% 41 21 4 100%
1091 P161274] 50 32 1 75% 21 10 3 92%
1092 P1612742 0 - 0 - 18 5 3 92%
1093 P1612743 86 5 3 100% 14 5 3 100%
1094 ° P1612744 34 4 3 92% 18 2 3 100%
1095 P1612746 15 0 2 88% 20 15 3 100%
1096 P1612747 36 9 4 88% 40 14 2 100%
1097 P1612748 4 4 5 90% 8 6 3 92%
1098 P1612749 57 3 3 100% 66 41 2 100%
1099 P1612750 76 O 2 100% 68 46 2 100%
1100 P1612752 31 5 2 75% - - 0 -
1101 P16127538 57 54 1 75% 8 6 5 100%
1102 P1612754 11 3 4 75% 0 0 3 100%
1 103 P16127588 75 66 2 75% - - 0 -
1104 P1612759D 16 14 2 75% 12 10 3 92%
1105 P1612760 33 14 3 75% 0 O 3 100%
1106 P16127618 12 4 2 63% 12 9 3 92%
1 107 P1548480 0 - 0 - 21 3 4 25%
1 108 P1548663 9 6 1 25% O 0 3 25%

 

P1: Plant Introduction, MG: Maturity Group, MAX: Maximum Number of Aphids per Leaflet
D1: Damaging Index, Mean: Average Number of Aphids on Three Leaflets

58

APPENDIX B

Germplasm used and data collected in 2002

59

 

 

 

Replication] Replication2
Entry PII FC
number MAX MEAN N°' °f DI MAX MEAN N°° °f D1
plants plants

1 P1548300 76 44.2 2 100% 20. 1 27 3 100%
2 P1548308 34 20.2 5 100% 5.5 12 3 100%
3 P1548312 10 8.2 5 100% 5.3 7 3 92%
4 P1548319 65 25.8 3 92% 7.4 16 3 75%
5 P1548328 14 9.7 4 88% 1.3 3 3 83%
6 P1548345 1 l .5 5 100% l .2 2 3 100%
7 P1548363 3 2.2 3 100% 1 .9 4 2 88%
8 P1548368 16 16.7 4 94% 5.7 1 1 3 83%
9 P154837] 52 37.0 4 94% 9.7 31 2 75%
10 P1548372 54 13 .0 5 100% 19.1 50 3 92%
1 1 P1548369 44 50.5 3 100% 0.0 0 3 75%
12 P1548370 56 37.8 4 100% 16.2 26 3 92%
13 P1548374 36 26.7 4 94% 24.1 39 3 92%
14 P1548375 30 17.5 5 80% 15.5 30 3 100%
15 P1548378 22 12.2 4 75% 14.0 20 3 92%
16 P1548380 l 1.8 4 100% 1.8 5 3 75%
17 P154839] 26 13.2 5 100% 10.9 17 1 75%
18 P1548396 33 16.2 2 100% 5 .0 9 3 92%
19 P1548406 6 8.2 4 100% 3.7 6 3 75%
20 P154841] 41 12.7 3 83% 18.4 27 2 75%
2] P1548424 - - O - 39.2 57 3 75%
22 FC001547 1 8.0 5 100% 11.4 31 3 83%
23 P1030594 98 56.3 1 100% 18.1 28 2 88%
24 P1030599 56 22.7 4 100% 19.5 27 3 100%
25 P1030600 4 1 .7 1 75% 4.2 9 3 92%
26 P1036653 30 17.2 3 100% 2.7 7 3 100%
27 P104713] 8 2.5 4 100% 4.6 19 3 100%
28 P1054604 4 6.0 4 100% 0.0 0 3 100%
29 P1054607 20 12.5 2 100% 7.6 21 3 100%
30 P1054608 16 12.7 2 88% - - 0 -
31 P1054608-1 65 26.3 4 56% 10.4 23 3 92%
32 P1054619 0 0.0 1 75% 42.5 90 3 100%
33 P1054818 14 10.7 4 100% 5.4 1 1 3 100%
34 P1054859 23 9.3 4 100% 0.0 0 3 100%
35 P1054862 12 19.2 4 100% 4.2 11 3 83%
36 P1054873 2 2.0 3 100% 11.3 18 3 83%
37 P1060279 52 28.8 2 100% 16.7 35 3 75%
38 P1065338 18 11.3 3 100% 4.0 12 3 75%
39 P106534] 10 3.3 1 75% 10.2 28 3 75%
4O P1065346 20 8.5 4 100% 0.0 0 3 100%
41 P1065354 27 5.7 5 100% 0.0 0 3 83%
42 P1065388 0 2.0 2 100% - - 3 100%
43 P106842] 17 4.2 5 100% 0.0 0 3 75%
44 P1068427 28 13.2 3 100% 8.2 15 2 75%
45 P1068430 1 1 13 .3 3 100% 2.8 5 2 100%

 

P1: Plant Introduction, MG: Maturity Group, MAX: Maximum Number of Aphids per Leaflet

DI: Damaging Index, Mean: Average Number of Aphids on Three Leaflets .

60

 

 

 

Replication] Replication2
Entry Pll FC N f N 1'
number MAX MEAN °' ° DI MAX MEAN °' ° DI
plants plants
46 P1068436 18 7.7 3 100% 0.6 2 2 100%
47 P1068439 1 18 .3 5 100% 6.7 1 8 3 92%
48 P1068443 30 23.5 5 100% 7.4 9 3 67%
49 P1068446 12 8.5 5 95% 8.4 19 3 100%
50 P1068448 0 16.0 2 100% 11.0 23 2 63%
5] P1068454 27 13.3 2 100% ' 10.3 30 2 88%
52 P1068455 37 18.7 5 100% 31.9 65 3 100%
53 P1068457 31 33 .5 5 100% 3 .4 10 3 100%
54 P1068457-1 12 16.2 4 100% 10.5 35 3 75%
55 P106846] 1 7.5 4 100% 4.3 14 2 63%
56 P1068461-l 4 3.7 4 81% 7.9 15 2 100%
57 P1068465 11 14.3 5 95% 13.8 21 2 88%
58 P1068465-1 62 45.5 2 100% 5.0 14 2 100%
59 P1068466 36 23.3 5 95% 10.0 20 3 100%
60 P1068474 50 22.8 4 100% 9.3 19 2 88%
6] P1068475 62 32.8 4 100% 8.9 12 3 83%
62 P1068475-l 17 9.0 5 100% 31.4 62 3 83%
63 P1068480 0 1 6 .0 5 100% 32 .8 50 3 67%
64 P106848] 20 31.8 4 100% 12.7 25 2 75%
65 P1068484-1 10 6.3 2 88% 7.0 15 3 75%
66 P10684844 10 14.5 4 100% 34.6 62 2 88%
67 P1068488 61 31.5 5 65% 54.7 80 3 75%
68 P1068500 4 3.8 5 100% 40.9 66 3 75%
69 P1068503 12 14.7 4 100% 76.1 105 3 67%
70 P1068508 39 18.3 3 92% 29.1 50 3 92%
7] P1068516 32 30.0 5 100% 36.1 62 3 83%
72 P106852] 65 37.2 3 100% 6.4 14 3 83%
73 P1068522 38 18.0 5 95% 8.3 18 2 100%
74 P1068526 l l 1 7 .2 4 100% 2.8 9 3 92%
75 P1068530 10 13.0 3 100% 46.0 62 3 92%
76 P1068543 65 26.0 4 100% 5 1 .3 62 3 92%
77 P1068551-2 2 1 17.2 5 90% l 1.3 22 3 92%
78 P1068555 28 6.2 3 92% 29.5 38 3 92%
79 P1068562 43 16.2 5 90% 51.8 62 3 75%
80 P1068564 101 43.8 5 100% 47.6 65 3 75%
8] P1068572 1 1 10.7 5 100% 6.6 1 1 3 92%
82 P1068585 16 10.7 3 92% 26.8 52 3 100%
83 P1068587 37 29.2 4 100% 42.6 122 3 100%
84 P1068598 35 10.5 3 100% 18.2 35 3 92%
85 P1068600 67 37.8 4 100% 28.3 32 1 75%
86 P10686098 28 1 1.7 3 92% 8.9 18 2 75%
87 P1068610 20 10.0 5 100% 7.5 23 3 100%
88 P1068622 13 7.2 4 63% 14.3 22 3 100%
89 P1068627 4 8.2 5 100% 4.4 9 3 83%
90 P1068629 29 1 1.7 4 88% 55.3 82 3 100%

 

61

 

 

 

Replication] Replication2
Entry ”I FC N r N 1
number MAX MEAN °' ° DI MAX MEAN °' ° DI
plants plants
9] P1068639 35 26.2 5 100% 67.2 87 3 92%
92 P1068642 32 21.0 3 92% 28.5 52 83%
93 P1068655 60 34.5 3 92% 18.3 22 1 75%
94 P1068658 17 5.7 4 100% 19.2 35 3 83%
95 P106866] 20 13.7 5 100% 17.8 50 3 100%
96 P1068663 39 20.8 2 100% 51.8 62 3 83%
97 P1068666 52 49.5 3 100% 14.9 26 2 100%
98 P1068670-1 6 3.3 2 100% 3.3 9 2 88%
99 P1068670-2 48 26.8 4 100% 4.3 12 3 83%
100 P106867] 26 13.0 5 100% 28.8 62 3 92%
101 P1068676 0 0.0 4 56% 5.0 9 1 100%
102 P1068680 40 18 .0 2 100% 89.5 150 3 100%
103 P1068680-2 10 5.3 4 94% 48.9 62 3 100%
104 P1068683 40 23.7 4 100% 114.5 150 1 100%
105 P1068685 53 26.3 4 100% 13.1 15 1 75%
106 P1068687 10 8.5 4 100% 0.5 3 3 92%
107 P1068694 1 1 4 .2 2 100% 9 1 .3 160 3 100%
108 P1068696 68 27.7 3 92% 95.1 170 3 100%
109 P1068704 71 39.2 4 100% 74 .9 150 3 100%
1 10 P1068706 70 61.0 3 100% 186.1 300 3 100%
1 1 1 P1068708 21 14.5 3 92% 25.6 41 3 92%
1 12 P1068709 101 63.5 5 100% 41.5 90 3 83%
113 P1068712 6 21.0 5 100% 6.8 12 3 92%
114 P1068713 58 21.0 5 100% 5.2 11 3 92%
115 P1068715 23 10.3 5 95% 3.5 11 3 100%
116 P1068718 21 23.2 5 100% 21.5 36 3 100%
1 17 P1068725 36 40.8 3 100% 22.4 62 2 100%
1 18 P1068728 6 4.5 5 100% 30.1 55 3 83%
1 19 P1068729 60 35 .7 3 100% 18.8 36 2 100%
120 P1068732 51 32.3 5 100% 17.5 22 3 92%
121 P1068736 39 31.2 3 100% 18.9 32 3 92%
122 8106874] 6 14.2 4 100% 24.0 50 3 ' 83%
1 23 P1068748 - - 0 - 47.7 68 2 100%
124 P106876] 15 10.8 5 100% 30.6 51 3 100%
125 P1068762 123 24.7 3 83% 9.8 18 2 100%
126 P1068763 24 17.5 4 100% 36.4 52 2 88%
127 P1068765 6 16.5 5 100% 65.5 150 2 100%
128 P1068770 12 13.8 4 94% 88.2 150 2 100%
129 P1068778 20 6.3 4 75% 22.5 31 1 100%
130 P1068788 5 6.2 4 63% 39.4 52 3 92%
131 P1068795 32 24.2 3 67% 25.2 38 2 100%
132 P1068815 28 24.2 2 100% 154.3 210 l 100%
133 P1069500 72 40.5 4 100% 39.3 58 3 92%
134 P106950] 80 23.7 4 100% 68.3 95 3 92%
13 5 P1069503 1 8 12.0 3 100% 24.4 36 3 92%

 

62

 

 

 

Replication] Replication2
Entry PU FC N f N 1'
number MAX MEAN °' ° D1 MAX MEAN °° ° DI
plants plants
136 P1069512 3 9.3 4 94% 23.5 46 3 83%
137 P1069532 41 29.0 2 88% 13.1 21 3 92%
138 P106999] 65 37.5 4 100% 18.3 42 3 83%
139 P1069992 75 38.7 2 100% 21.7 32 2 88%
140 P1069996 76 39.5 2 100% 10.8 20 3 100%
141 P1070009 73 42.0 5 100% - - 1 100%
142 P1070016 12 4.5 4 100% 12.0 18 3 92%
143 P1070017 34 35.2 5 95% 29.8 52 2 100%
144 P107002] 25 18.0 4 100% 8.3 22 3 92%
145 P1070027 10 6.3 1 100% 5.4 18 3 100%
146 P1070036 41 30.7 4 100% 30.5 61 3 92%
147 P1070077 60 41.8 4 81% 9.6 18 3 92%
148 P1070078 23 22.3 4 100% 0.0 0 3 83%
149 P1070084 2 9.2 3 83% 27.4 46 3 83%
150 P1070087 17 6.0 3 100% 35.4 57 2 100%
151 P1070089 10 5.2 5 85% 47.4 67 2 75%
152 P107009] 16 10.8 2 100% 53.3 88 3 100%
153 P1070197 15 9.3 4 94% 14.6 45 2 88%
154 P1070224 52 35.3 3 100% 43.8 63 2 100%
155 P1070228 14 21.2 5 100% 36.5 48 3 92%
156 P107024] 18 8.7 2 100% 40.7 59 3 100%
157 P1070242 18 23.7 5 100% 41.9 73 3 100%
158 P107025] 2 18.0 4 100% 19.8 35 2 100%
159 P1070453 44 31.7 2 i 100% 39.3 66 3 100%
160 P1070456 40 35.0 4 100% 13.9 19 1 75%
161 P1070457 34 34.5 3 92% 21.5 26 1 75%
162 P1070458 75 51.0 4 94% 46.1 78 3 100%
163 P1070459 103 52.8 4 100% 47.1 69 3 92%
164 P1070460 91 54.2 3 92% 1.7 4 3 75%
165 P107046] 62 47.2 4 100% 17.8 28 3 75%
166 P1070463 70 54.5 3 83% 6.4 14 3 83%
167 P1070476 58 49.3 4 94% 19.2 28 3 92%
168 P1070478 28 22.8 2 100% 42.3 46 2 100%
169 P1070485 55 42.5 3 100% 26.6 34 3 92%
170 P1070495 76 43.0 4 100% 38.4 70 2 100%
171 P1070503 103 64.2 3 100% 38.5 42 3 92%
172 P1070507 120 67.5 3 92% 34.6 43 3 92%
173 P1070516 28 26.0 5 100% 49.4 61 3 100%
174 P1070520 58 13.5 5 100% 34.3 48 3 92%
175 P107056] 41 19.7 3 83% 39.4 62 3 83%
176 P1071850 32 19.0 4 100% 31.5 39 3 75%
177 P1072328 6 5.8 5 100% 28.0 38 3 83%
178 P1072337 34 33 .5 4 94% 51.1 61 3 92%
179 P107234] 90 31.8 4 100% 4.0 12 3 92%
180 P1072342 122 66.3 4 100% 32.3 45 2 88%

 

63

 

 

 

Replication] Replication2
Entry ”I FC N r N 1
number MAX MEAN °' ° D1 MAX MEAN °' ° 81
plants plants
181 P1073583 78 39.5 4 100% 34.1 51 3 92%
182 P1073585 26 26.7 3 100% 20.9 38 2 100%
183 P1073587 30 18.2 5 100% 13.6 19 2 100%
184 P1073772 42 21.0 5 100% 14.3 18 3 92%
185 P1073780 60 27.8 5 100% 19.4 28 3 92%
186 P1079586 61 52.3 1 100% 31.9 41 3 92%
187 P1079593 24 18.3 5 100% 1 1.9 18 3 75%
188 P1079596 28 40.5 5 95% 11.5 15 3 92%
189 P1079602 42 35.2 4 100% 35.4 41 3 92% '
190 P1079609 61 44.3 4 94% 39.1 51 3 75%
191 P1079610 75 59.0 2 100% 47.0 60 2 75%
192 P1079613 92 64.7 4 94% 48.1 60 3 75%
193 P1079694 83 63.8 5 295% 0.0 0 1 75%
194 P1079695 101 61.7 2 100% - - 0 -
195 P1079699 62 48.7 5 100% 15.8 21 3 83%
196 P1079703 58 45.5 3 100% 33.3 38 3 83%
197 P1079712 42 39.3 3 100% 50.1 61 3 83%
198 P1079727 80 89.3 5 100% 1 1.0 18 2 75%
199 P1079737 37 33.0 5 95% 10.2 14 3 33%
200 P1079745 - - 0 - 0.0 0 3 75%
201 P1079746 - - 0 - 1 8. 1 24 3 100%
202 P1079747 50 54.3 2 100% 13.2 21 2 88%
203 P1079756 20 19.8 5 100% 7.9 18 3 75%
204 P107976] 16 6. 8 3 100% 28.1 38 2 100%
205 P1079773 12 25.5 2 100% 53.1 63 3 100%
206 P1079846 58 25.2 3 92% 28.2 37 3 92%
207 P1079848 32 34.0 2 88% 0.0 0 3 25%
208 P1079862-1 34 20.7 1 100% 0.0 O 3 25%
209 P1079863 84 51.3 4 100% 61.5 71 3 92%
210 P1079885 48 44.8 3 92% 9.1 18 3 83%
2]] P1080536 61 53.0 2 88% 40.7 56 2 88%
212 P1081763 20 31.0 5 95% 0.0 0 3 75%
213 P1081767 101 76.7 3 92% 11.6 14 2 88%
214 P1081768 90 108.2 4 100% 8.3 18 3 92%
215 P1081770 72 59.3 5 90% 29.7 34 3 75%
216 P108177] 12 54.2 4 100% 19.6 28 3 75%
217 P108567] 56 47.8 5 100% 5.2 16 3 67%
218 P108674] 169 1 17.2 3 100% 24.2 30 3 83%
219 P1086878 53 23.7 4 100% 4.3 12 3 92%
220 P1086878-2 58 17.2 5 100% 0.0 O 3 100%
221 P1087524 2 2.5 5 100% 5.3 8 3 67%
222 P1088288 6 7. 8 5 100% 17.0 23 3 75%
223 P1088293 10 12.3 4 94% 13.0 17 3 75%
224 P1088293A 22 1 1.3 4 94% 46.7 52 l 50%
225 P1088294 88 57.8 1 100% 26.9 40 3 75%

 

64

 

 

 

Replication] Replication2
Entry PU FC N 1 N 1
number MAX MEAN °‘ ° DI MAX MEAN °° ° DI
plants plants

226 P1088294-1 75 67.5 5 100% 37.5 52 3 75%
227 P1088295-1 58 50.5 4 100% 1.0 2 3 92%
228 P1088296 17 12.7 1 75% 1.8 6 3 100%
229 P1088298 72 51.0 4 100% 3.5 5 3 92%
230 P108830] 67 35.8 3 100% 4.2 7 3 75%
231 P1088303 75 38.7 4 88% 24.8 30 2 88%
232 P1088304 8 16.8 2 100% 10.7 25 2 75%
233 P1088307 33 15.0 4 100% 29.1 41 3 67%
234 P1088308 44 28 .0 3 100% 15 .0 26 3 42%
235 P1088309 28 21.7 2 100% 25.8 41 3 83%
236 P108831] 21 15.3 4 94% 2.3 3 3 58%
237 P1088313 21 15.7 4 94% 41.1 51 3 100%
238 P108835] 93 46.0 5 100% 45.4 56 3 92%
239 P1088352 43 60.8 2 100% 47.5 70 2 100%
240 P1088355 - - 0 - 16.4 27 3 83%
241 P1088356 - - 0 - 13 .8 2 1 3 100%
242 P1088357 1 5 9.7 5 100% 21.8 40 3 83%
243 P1088358 22 37.7 5 95% 43.0 51 2 100%
244 P1088442 3 3 22.8 4 100% 40.8 51 3 83%
245 P1088455 36 25.2 2 100% 36.4 47 3 83%
246 P1088468 26 12.8 5 100% 44.9 56 2 88%
247 P1088479 85 68.3 5 90% 47.0 60 3 83%
248 P1088495 18 19.2 5 100% 13.0 35 2 75%
249 P1088508 1 4.0 4 100% 51 .9 60 3 92%
250 P1088777 38 40.2 5 95% 46.2 70 3 100%
25 1 P1088787 78 40.7 5 100% 14.4 18 3 100%
252 P1088798 69 16.7 5 100% 6.0 16 3 83%
253 P1088803 66 25.3 3 100% 29.0 35 3 83%
254 P10888054 9 59.3 5 100% 5.0 16 2 88%
255 P1088997 31 30.5 4 100% 7.8 18 3 83%
256 P1089000 18 16.7 3 100% 32.2 40 3 92%
257 P1089003-1 60 42.3 5 100% 22.1 30 3 100%
258 P1089004 12 20.2 5 100% 6.7 16 3 92%
259 P1089005-5 59 41.5 3 100% 5.9 18 3 100%
260 P1089006-2 78 56.2 4 94% 19.7 25 3 75%
261 P1089008 98 76.0 3 100% 12.4 18 3 100%
262 P1089012 44 41.8 3 92% 20.6 39 3 100%
263 P1089013 101 79.7 3 83% 9.0 18 3 67%
264 P1089014 62 72.3 4 88% 23.1 30 3 83%
265 P1089053 39 39.0 4 100% 19.1 28 3 83%
266 P1089055-1 33 28.8 3 92% 14.4 20 3 75%
267 P1089059 31 15.2 5 90% 22.5 30 2 88%
268 P1089063 12 20.5 5 100% 12.4 18 3 83%
269 P1089064 53 49.8 3 92% 0.0 0 3 83%
270 P1089065 47 43.5 3 100% - - 0

 

65

 

 

 

Replication] Replication2
Entry ”I FC N f N 1’
number MAX MEAN °‘ ° DI MAX MEAN °' ° D1
plants plants
27] P1089065-2 21 12.2 2 88% 28.8 40 1 75%
272 P1089070 61 26.0 3 92% 9.0 18 3 83%
273 P1089072 26 15.8 4 100% 5 .0 8 3 83%
274 P1089073 44 30.7 5 90% 3.1 8 3 75%
275 P1089075 107 72.2 3 92% 8.8 18 3 92%
276 P1089167 23 31.0 4 100% 9.8 19 3 83%
277 P1089170 55 43.5 4 88% 1 1.9 16 3 92%
278 P108917] 14 34.7 4 100% 19.3 32 2 100%
279 P1090560 88 44.7 2 100% 15 .8 42 3 100%
280 P1090567 61 34.0 3 92% 5.2 12 2 100%
281 P1090570 90 25.5 4 100% 15.8 23 3 100%
282 P1090574 7 6.0 1 100% 14.3 21 3 100%
283 P1090575 33 36.7 5 100% 12.2 18 3 92%
284 P109109] 49 46.5 3 100% 10.8 25 3 100%
285 P1091102 20 41.2 4 88% 6.5 18 2 88%
286 P1091104 37 49.3 4 100% 0.0 0 3 42%
287 P1091107 58 47.0 5 95% 10.9 18 3 92%
288 P1091109 9 33.8 5 100% 17.9 34 3 92%
289 P1091110 77 21.2 4 100% 26.3 31 3 92%
290 P1091110-1 102 88.8 3 100% 10.9 21 3 100%
291 P1091114 15 13.5 2 88% 11.8 18 3 92%
292 P1091115 150 81.8 4 100% 7.8 15 3 100%
293 P1091116 52 39.2 3 100% 16.8 26 3 100%
294 P1091 117 58 27.5 4 100% 10.8 25 3 75%
295 P1091119 22 36.0 4 100% 11.5 29 3 92%
296 P1091120 68 46.7 4 94% 0.0 0 3 100%
297 P1091120-2 89 52.0 5 100% 6.0 18 3 92%
298 P1091123 23 32.7 4 100% 8.0 18 3 100%
299 P1091124 63 65.0 5 90% 4.1 8 3 100%
300 P1091126 99 53.3 5 100% 16.8 28 1 75%
301 P1091129 21 15.5 4 94% 0.9 2 2 100%
302 P1091132-2 26 ' 25.7 5 100% 8.8 18 3 25%
303 P1091138 89 52.3 4 94% 6.4 14 3 100%
304 P109114] 59 56.5 5 100% 0.0 0 2 100%
305 P1091144 36 42.8 5 90% 1.5 4 2 100%
306 P1091150 60 39.0 3 100% 0.0 0 3 100%
307 P1091156 8 2.8 5 95% 15.7 23 2 100%
308 P109] 16] 1 13 67.2 4 100% 13.4 19 3 92%
309 P1091164 98 61.3 5 90% 62.5 70 3 ~ 83%
310 P1091167 - - 0 - 38.9 45 1 75%
31] P109117] 39 57.8 4 94% 2.8 8 3 100%
312 P1091180 119 104.8 5 100% 0.0 0 3 25%
313 P1091557 75 80.8 5 100% 3.0 8 3 100%
314 P1091559 130 101.3 1 100% 6.9 18 2 100%
315 P1091732-1 41 38.2 2 88% 4.4 9 3 75%

 

66

 

 

 

Replicationl Replication2
Entry PU FC N f N 1'
number MAX MEAN °' ° D1 MAX MEAN 0' ° DI
plants plants

316 P1091732-2 8 36.5 3 100% 5.4 11 3 75%
317 P1091733 51 23.8 4 100% 2.6 5 3 83%
318 P109256] 120 88.0 2 100% 10.1 26 2 88%
319 P1092563 106 76.2 5 100% 0.0 0 3 58%
320 P1092565 36 37.5 4 94% 9.8 21 3 67%
321 P1092569 98 56.7 5 95% 5.5 9 3 67%
322 P1092570 81 48.7 3 100% 0.0 0 3 58%
323 P109257] 53 47.3 2 88% 0.0 0 2 63%
324 P1092572 37 15.2 3 92% 4.5 9 2 88%
325 P1092573 69 65.2 5 100% 0.0 0 2 75%
326 P1092576 28 27.2 3 92% 0.0 0 3 75%
327 P1092580 18 19.2 4 94% 0.0 0 3 58%
328 P1092582 51 21 .7 3 100% 0.0 0 3 67%
329 P1092583 32 32.3 5 95% 0.0 0 2 63%
330 P1092589 54 38.3 4 94% 31.7 48 2 88%
331 P1092592 65 36.8 5 100% 5.7 1 1 3 92%
332 P1092595 15 25.7 4 94% 6.2 1 1 3 100%
333 P1092596 23 25.3 3 100% 10.2 15 2 88%
334 P1092598 40 17.7 0 - 4.5 9 3 67%
335 P1092603 26 1 1.0 4 94% 0.0 0 3 67%
336 P1092607 82 58.7 3 100% 0.0 0 3 67%
337 P109261] 91 43.7 5 100% 0.0 0 2 75%
338 P1092625 68 33.8 2 88% 0.0 0 3 25%
339 P1092627 65 38.2 3 100% 3.8 12 2 75%
340 P1092629 78 35.5 5 90% 9.9 18 2 88%
341 P1092630 32 64.5 3 92% 3.2 12 2 100%
342 P1092633 1 12 78.3 1 100% 16.1 30 3 100%
343 P1092639 38 17.7 5 100% 6.9 12 3 100%
344 P1092649 30 10.0 2 88% 3.7 8 3 83%
345 P1092660 40 32.0 1 100% 28.5 32 1 100%
346 P109266] 42 30.0 1 100% - - 0 -
347 P109267] 60 45.3 1 100% 25.6 32 1 100%
348 P1092677 52 28.7 5 100% 10.0 15 3 83%
349 P109268] 81 76.0 1 100% 16.8 20 1 100%
350 P1092683 0 8.7 4 100% 7.0 15 3 100%
351 P1092684 14 25.2 5 100% 1.6 13 3 58%
352 P1092687 43 26.0 3 92% 30.8 54 3 92%
353 P1092694 30 28.7 3 92% 0.0 0 3 92%
354 P1092696 l 1 4.2 5 95% 8.9 16 3 92%
355 P1092698 12 20.3 4 100% - - O -
356 81092705 15 30.3 1 100% 12.7 18 l 100%
357 P1092706 81 52.5 3 100% 2.3 12 3 83%
358 P1092717 71 33.5 5 95% 14.2 22 3 75%
359 P1092719 52 33.0 4 100% 12.3 21 3 92%
360 P1092733 46 3 8 .0 4 94% 9. 5 21 2 100%

 

67

 

 

 

Replication] Replication2
Entry W FC N r N 1
number MAX MEAN °° ° D1 MAX MEAN °° ° 81
plants plants

361 P1092734 72 35.2 5 100% 6.9 13 3 92%
362 P1093559 28 42.3 4 100% 8.5 17 3 92%
363 P1093560 85 30.8 3 92% 1 1.5 17 3 83%
364 P1093565 71 40.8 3 100% 1.3 4 3 25%
365 P1096188 65 35.0 4 100% 18.6 22 3 100%
366 P1096195 56 49.8 4 94% 18.2 32 3 100%
367 P1096201 - - 0 - 24.8 36 2 88%
368 P1103414 101 76.5 2 100% 24.4 42 3 100%
369 P1135589 101 60.8 4 100% 17.5 32 3 100%
370 P1135590 30 11.5 5 95% 27.4 36 3 92%
371 P1142491 69 39.8 5 100% 22.6 30' 3 83%
372 P1200592 11 1 88.0 3 92% 10.8 16 3 100%
373 P1200596 92 79.8 4 94% 18.8 23 3 92%
374 P1232987 15 34.3 5 100% 24.5 42 3 83%
375 P1232988 20 24.0 2 100% 22.3 25 1 100%
376 P1232989 0 0.3 5 100% 6.3 13 3 83%
377 P1232990 56 20.3 4 100% 14.3 26 3 83%
378 P1232991 1 0.8 4 100% 16.9 25 3 83%
379 P1261474 27 24.0 3 92% 33.2 53 3 100%
380 P1266085A 13 13.8 3 100% 8.4 23 3 100%
381 P12660858 93 58.7 3 100% 6.5 15 3 83%
382 P1266085C 21 13.3 1 100% 20.9 41 3 100%
383 P1266806A 19 4.0 2 88% 21.0 31 3 83%
384 P12668068 17 18.5 2 100% 19.3 25 3 100%
385 P1266806C 14 2.5 5 100% - - 0 -

386 P1266807A 56 21.5 2 100% 19.6 24 3 92%
387 P12668078 54 16.8 4 100% 7.1 17 3 92%
388 P1291272 0 3.2 4 100% 7.7 10 3 75%
389 P1291273 o 22.7 4 100% 3.7 8 3 92%
390 P1291274A 33 1 1.3 5 95% 0.0 0 3 25%
391 P12912748 23 14.8 5 100% 12.1 28 3 75%
392 P1291275 56 37.3 3 100% 6.2 15 3 75%
393 P1291276 112 51.5 5 100% 6.8 16 3 75%
394 P1291277 15 8.5 5 100% 2.2 7 3 75%
395 P1291278 12 10.5 5 100% 18.8 32 3 100%
396 P1291279 80 19.3 3 100% 8.1 18 3 92%
397 P1291281 14 7.0 4 100% 5.5 16 3 92%
398 P1291282 27 13.7 4 100% 1.5 10 3 92%
399 P1291283 14 6.3 4 100% 0.3 1 3 75%
400 P1291284 11 9.5 3 100% 8.3 17 3 83%
401 P1291286 64 26.7 5 100% 1.8 6 3 75%
402 P1291288 26 13.2 2 100% 3.0 9 3 58%
403 P1291290 165 59.7 3 100% 5.2 17 3 83%
404 P1291291 23 11.0 1 100% 0.3 1 3 83%
405 P1291293A 196 77.7 4 100% 4.4 12 3 75%

 

68

 

 

 

 

Replicationl Replication2
Entry PI/ FC
number MAX MEAN N°' “f DI MAX MEAN N“ ”f DI
plants plants

406 P12912938 50 23.8 4 100% 7.9 24 3 100%
407 P1291294 37 12.0 4 94% 9.1 21 2 88%
408 P1291295 8 16.0 4 100% 12.5 38 2 75%
409 P1291296 32 17.0 1 100% 13.9 30 3 92%
410 P1291297 21 13.8 4 100% 7.3 17 3 92%
411 P1291298 38 29.8 3 100% 8.5 23 3 75%
412 P1291299 19 21.2 5 100% 3.3 8 3 75%
413 P1291300 22 7.5 4 100% 0.9 3 2 50%
414 P1291301 37 10.5 5 95% 7.4 20 3 92%
415 P1291302A 0 0.8 4 81% 4.2 14 3 75%
416 P12913028 0 2.3 3 100% 5.3 18 3 25%
417 P1291302C - - 0 - - - 0 -

418 P1291303A 17 12.7 5 100% 14.1 44 3 83%
419 P12913038 22 9.3 5 100% 2.5 8 2 100%
420 P1291304 27 34.3 2 100% 6.8 10 3 92%
421 P1291305 104 53.5 3 92% 1 1.7 29 2 100%
422 P1291306A 7 1.5 5 100% 10.8 27 2 75%
423 P1291309A 5 3.8 3 100% 6.3 21 2 75%
424 P12913098 17 3.5 4 100% 10.6 30 3 58%
425 P1291309C 89 30.2 5 95% 7.1 24 3 75%
426 P1291309D 1 15 88.8 5 90% 18.1 29 3 100%
427 P1291310A 64 42.5 4 100% 17.9 45 3 92%
428 P12913108 23 6.5 4 100% 22.8 32 3 92%
429 P1291310C 128 87.3 5 100% 11.7 27 2 75%
430 P12913118 80 27.8 4 88% 19.0 27 2 100%
431 P1291315 66 48.3 5 300% 10.0 17 2 100%
432 P1291320A 59 20.7 3 100% 25.6 48 3 100%
433 P1291322 22 15.3 1 100% 23.0 43 2 100%
434 P1291323 26 44.5 2 100% 13.8 26 3 83%
435 P1291324 78 75.7 2 100% 6.2 18 3 83%
436 P1291327 67 60.7 4 100% 20.7 33 3 100%
437 P1291328 30 19.7 5 100% 3.3 10 2 100%
438 P1297500 117 68.7 4 100% 8.0 13 2 100%
439 P1361074 120 97.0 5 100% 12.0 18 3 100%
440 P1361075 140 101.7 4 100% 9.9 19 3 100%
441 P1372404A - - 0 - 29.3 48 3 100%
442 PI372404C 1 17 90.7 4 94% 5.1 12 3 100%
443 P1383276 69 32.2 4 100% 5.4 12 3 100%
444 P1383277 48 55.2 3 92% 23.1 50 3 100%
445 P1383278 15 6.3 5 100% 7.5 14 3 100%
446 P1391577 68 32.0 2 75% 16.9 22 1 100%
447 P1391578 - - 0 - 19.2 32 3 100%
448 PI391581A 97 52.5 2 100% 31.5 52 3 100%
449 P13915818 72 52.5 3 100% 26.1 33 3 100%
450 P1391583 105 68.7 4 94% 18.9 51 3 83%

 

69

 

 

 

Replication] Replication2
Entry PU FC N r N 1
number MAX MEAN °' ° DI MAX MEAN °' ° DI
plants wants
451 P1391584 78 56.2 3 100% 13.1 23 3 100%
452 P1391585 30 17.3 4 100% 14.7 35 3 100%
453 P1391589A 98 72.8 4 100% 18.8 39 3 100%
454 P13915898 68 54.3 2 88% 25.0 58 2 100%
455 P1391594 19 12.5 2 75% 17.1 35 2 100%
456 P13935418 41 39.2 4 100% 11.5 21 3 100%
457 P1393563 133 92.8 3 100% 20.3 33 3 100%
458 P1393999 120 111.7 1 100% 22.4 35 3 100%
459 P1405690 107 56.3 5 100% 44.0 55 2 88%
460 P140770] - 0 31.3 43 3 83%
461 P1407704 78 37.2 3 92% 41.5 57 3 92%
462 P1407705 1 10.7 3 100% 20.5 25 l 75%
463 PI407706A 16 1 1.0 1 100% 20.5 30 2 100%
464 P14077068 136 79.7 1 100% 19.0 26 3 100%
465 P1407710 96 27.0 2 100% 17.7 22 3 100%
466 P1407711A 122 112.7 1 100% 19.4 31 3 100%
467 P14077118 - - 2 88% 0.0 0 3 25%
468 P1407712 - - 0 - 15.8 21 3 100%
469 P1407714 56 32.2 3 100% 12.6 22 2 88%
470 P1407715 120 65.7 4 88% 16.9 26 3 100%
471 P1407716 75 53.2 2 100% 16.8 27 3 100%
472 P1407717 130 71.5 3 92% 30.5 40 3 100%
473 P1407718 124 84.2 2 100% 21.2 30 3 100%
474 P1407719 77 43.3 2 100% 13.6 20 3 100%
475 P1407720 98 92.3 4 100% 14.8 19 3 100%
476 P140772] 1 17 112.0 1 100% 12.0 16 3 100%
477 P1407723 69 52.8 2 100% 20.8 32 3 100%
47 8 P1407724 101 24.2 5 100% 10.4 12 3 100%
479 P1407726 23 14.5 2 100% 1 1.2 18 3 83%
480 P1415072 16 11.7 1 100% 30.2 35 3 83%
481 P14150738 23 19.3 2 100% 0.0 0 3 100%
482 P1415074 12 17.3 5 100% 5.8 12 3 100%
483 P142420] 12 41.2 2 100% 14.2 32 3 100%
484 P1424204 17 44.5 2 100% 0.0 0 3 100%
485 P1427088A 25 19.0 1 100% 10.4 26 3 100%
486 P14270888 18 16.3 4 100% 9.8 29 3 83%
487 PI427088C 1 6.3 5 100% 4.0 9 3 83%
488 P1427088D 30 18.5 4 88% 23.5 50 3 83%
489 P14270888 40 68.8 4 100% 8 .6 18 3 83%
490 P1427088F 52 27.8 4 100% 4.8 9 3 100%
491 P14270880 13 5.0 2 75% 20.1 29 3 100%
492 P14270881] 21 4.8 2 100% 12.0 24 3 100%
493 P14270881 20 14.3 4 100% 0.0 0 1 25%
494 P1427099 63 35.5 2 100% 10.9 34 3 100%
495 P1427105A 91 48.7 4 100% 4.1 6 3 100%

 

PI: Plant Introduction, MG: Maturity Group, MAX: Maximum Number of Aphids per Leaflet
DI: Damaging Index, Mean: Average Number of Aphids on Three Leaflets

70

 

 

 

Replication] Replication2

8nt PI/ FC

ry number MAX MEAN N°' ”f 81 MAX MEAN N°‘ ”f DI
plants Jlants

496 P14271058 1 1 13.5 3 100% 5.0 18 2 100%
497 P1427106 72 . 52.7 1 100% 2.3 8 2 88%
498 PI427107A 98 84.3 3 100% 21.6 35 3 100%
499 P14271078 18 21.8 3 100% 25 .0 44 3 92%
500 PI427107C 17 30.2 3 100% 1.2 4 3 100%
501 P1430460A 13 12.5 5 100% 13.9 37 3 100%
502 P14304608 28 23.3 4 100% 10.3 26 3 100%
503 P1436617 35 16.8 5 100% 8.4 15 3 100%
504 P143668] 65 53.8 4 100% 6.3 12 3 100%
505 P1436682 42 26.0 4 94% 8.8 14 3 100%
506 P1436683 48 14.5 5 100% 7.0 16 3 92%
507 P1436684 10 3.2 2 88% 0.0 0 3 67%
508 PI437105A 18 10.2 4 94% 18.1 42 3 92%
509 P14371058 5 13.5 5 100% 18.8 26 3 83%
510 P1437105C 28 7.8 5 100% 1.0 2 3 75%
51 1 P14371058 40 27.2 4 94% 14.1 33 3 83%
512 P1437568 72 32.5 4 100% 28.3 47 3 75%
513 P1437569 85 43.5 2 100% 9.4 15 3 100%
514 P1437572 2 16.2 5 100% 2.0 7 3 83%
515 P1437589 45 22.8 5 100% 13.5 28 3 50%
516 P1437656 48 27.5 4 100% 16.9 21 3 75%
517 P1437662 25 19.0 4 94% 4.8 10 3 75%
518 P1437663 17 13.5 4 100% 28.9 68 3 100%
519 P1437664 45 42.2 5 100% 0.0 0 3 25%
520 P1437722 120 95.0 . 2 100% 0.0 O 3 25%
521 P14377388 130 137.5 5 100% 0.0 0 3 25%
522 PI437814A 120 112.5 3 100% 0.0 0 3 25%
523 P14378148 55 33.5 3 100% 0.0 0 3 25%
524 P1437815 40 100.0 2 100% 1.5 5 3 25%
525 P1437817 9 18.5 3 100% 0.0 0 3 25%
526 P1437818A - - 0 - 6.3 16 3 25%
527 P14378188 20 37.5 5 50% 0.0 0 3 25%
528 P1437819 80 115.0 1 75% 20.8 24 1 75%
529 P1437820 40 42.5 5 90% 0.0 0 3 25%
530 P143782] 1 30.5 4 94% 0.0 0 3 25%
531 P1437822 15 7.5 5 90% 0.0 0 3 25%
532 P1437823 25 40.0 4 100% 0.0 0 3 25%
533 P1437824 16 38.0 4 100% 0.0 0 3 25%
534 P1437825 50 85.0 5 90% - - 3 25%
535 P1437827 1 1 6.0 3 100% 0.0 0 3 25%
536 P1437828 1 1 1.0 3 100% - - 3 25%
537 P1437905 30 52.5 2 100% 0.0 0 3 25%
538 P1437913 15 17.5 5 100% 3.3 5 3 50%
539 P1452433 300 - 4 100% 3.2 5 3 33%
540 P1458505 10 15.0 4 81% 4.7 11 3 25%

 

71

 

 

 

Replication] Replication2
Entry PU FC N f N 1'
number MAX MEAN °' ° D1 MAX MEAN °' ° D1
plants plants
541 P1458506 25 18.5 5 100% 10.3 22 3 58%
542 P1458507 4 2.0 5 75% 3.7 6 3 25%
543 PI458508A 65 47.5 4 100% 2.2 7 3 58%
544 P14585088 0 7.5 5 100% 6. 1 14 3 75%
545 P145851] 40 20.0 5 100% 0.0 0 3 25%
546 P1458512 50 32.5 3 100% 14.3 30 3 92%
547 P1458517 5 2.5 2 88% 6.1 8 1 75%
548 P1458519A 85 42.5 4 100% 10.0 20 3 100%
549 P1458520 20 30.0 4 100% 1 1.1 21 3 100%
550 P1458522 5 15.0 5 85% 21.7 36 3 92%
551 P1458525 45 45.0 3 100% 29.5 55 3 75%
552 P1458529 12 8.5 4 100% 10.5 31 3 92%
553 — P14585328 0 15.0 4 100% 13.4 38 3 92%
554 P145854] 0 12.5 5 100% 12.9 37 3 100%
555 P1458829 0 5.0 5 100% 24.4 31 3 100%
556 P1461508 5 2.5 4 100% 18.0 25 2 100%
557 P1461509 1 1.5 4 94% 11.7 25 3 83%
558 P1464865 12 12.0 1 100% 6.0 13 2 100%
559 P1464878 10 25.0 2 100% 17.3 46 3 83%
560 P1464880 10 8.0 2 100% 8.0 12 3 100%
561 P1464882 12 6.0 5 100% 18.8 29 3 83%
562 P1464883 16 13.0 4 94% 23.3 50 3 92%
563 P1464884 3 21.5 2 100% 11.9 23 3 92%
564 P1464887 20 25.0 3 100% 14.8 26 3 83%
565 P1464888A 20 20.0 3 100% 10.4 17 3 83%
566 P14648888 15 8.0 5 100% 28.0 73 3 100%
567 P1464893 3 3.0 3 100% 15.9 53 3 100%
568 P1464894 10 3.2 2 100% 5.6 14 3 100%
569 P1464895 - - 0 - 0.0 0 3 100%
570 P1464896 20 9.3 3 100% 1 1.2 32 3 100%
571 P1464897 2 7.7 3 100% 0.0 0 3 33%
572 P1464898 - - 0 - 26.0 45 3 100%
573 P1464899 43 22.2 2 100% 7.4 14 3 100%
574 P146490] 35 17.3 4 100% 26.9 50 3 100%
575 P1464904 19 7.8 5 100% 12.5 29 3 100%
576 P1464905 38 16.8 3 100% 8.8 18 3 100%
577 P1464909 38 41.7 5 100% 33.3 45 2 88%
578 P1464914A 14 16.0 3 83% 24.4 36 2 100%
579 P1464914A 8 4.8 3 100% 19.7 24 3 100%
580 P1464915A 10 10.7 4 100% 15.8 32 2 88%
581 P14649158 24 18.5 3 100% 29.5 41 3 92%
582 P1464917 49 18.0 4 100% 32.3 41 2 100%
583 P146492] 13 14.8 3 100% 42.3 52 3 92%
584 P1464922 10 10.0 1 100% 25.0 40 2 100%
585 P1464924 40 47.5 2 100% 36.9 50 2 100%

 

72

 

 

 

Replication] Replication2
Entry PU FC N r N 1
number MAX MEAN °° ° DI MAX MEAN 0' ° D1
plants plants
586 P1464940 40 40.0 1 100% 38.3 52 3 100%
587 P146494] 60 42.5 5 100% 18.0 38 2 100%
588 P1467307 5 20.0 3 100% 27.2 32 2 88%
589 PI467308A 4 5.5 3 100% 39.3 50 2 100%
590 P14673088 30 25 .0 3 100% 26.0 30 2 88%
591 P1467310 45 52.5 4 100% 20.1 40 2 88%
592 P1467311A 250 - 2 100% 21.1 36 3 100%
593 P14673118 60 60.0 3 100% 26.6 41 3 100%
594 P146731 1C 225 225.0 1 100% 17.9 23 1 75%
595 P14673118 20 20.0 1 100% 28.8 41 2 100%
596 P14673118 40 35.0 5 100% 23.8 36 3 75%
597 P1467312 30 25.0 5 100% 6.8 21 1 25%
598 P1467314 20 22.5 5 100% 22.3 28 3 92%
599 P1467315 1 20.5 5 100% 20.8 32 2 100%
600 P1467317 120 100.0 4 88% 20.4 30 3 92%
601 P1467318A 54 39.3 1 100% 18.0 26 3 100%
602 P14673188 32 12.4 5 80% 10.5 16 3 92%
603 P1467320 38 22.8 3 g 100% 24.7 40 3 100%
604 P146732] 5 7.8 2 100% 27.1 36 3 100%
605 PI467322A 32 22.0 1 75% 1 1.0 21 3 92%
606 P14673228 18 13 .8 2 100% 8.3 15 2 100%
607 P14673238 21 10.3 5 100% 8.8 21 3 100%
608 P1467324 6 3 .2 4 100% 24.6 38 3 100%
609 P1467325 36 15 . 8 2 100% 15 .2 24 3 100%
610 P1467327 62 37.0 4 100% 23.7 45 3 92%
61 1 P1467328 8 4.5 3 92% 17.0 28 3 92%
612 P1467329 49 12.8 5 100% 19.0 27 2 100%
613 P1467330 1 2.7 2 100% 17.0 22 3 92%
614 P146733] 13 9.5 4 100% 16.5 26 3 100%
615 P1467332 8 5.2 3 75% 16.5 32 3 83%
616 P1467333 38 39.2 5 100% 11.9 15 3 83%
617 P1467334A 5 8 49.5 2 100% 6.7 15 2 100%
61 8 P14673348 - - 0 - 8 .6 1 5 1 100%
619 PI467335A 72 31.0 5 100% 22.1 32 3 92%
620 P14673358 102 82.7 2 100% 14.9 21 2 100%
621 P1467336 92 41.3 2 100% 1 1.7 21 2 100%
622 P1467337 8 14.0 4 100% 9.7 12 3 100%
623 P1467338 20 19.2 2 100% 0.3 1 3 50%
624 P1467339 36 15 .0 5 100% 12.7 26 3 100%
625 P1467340 70 39.3 4 94% 9.0 13 3 92%
626 P146734] 100 69.3 2 88% 7.4 22 3 100%
627 P1467342 30 31.5 4 94% 12.7 17 3 100%
628 P1467343 79 56.3 3 92% 30.2 45 3 100%
629 P1467346 52 30.8 4 100% 8.0 18 3 100%
630 P1467347 27 22.5 3 100% 0.3 1 1 75%

‘T

 

 

73

 

 

 

Replication] Replication2
Entry “I FC No of ' No of
number MAX MEAN ° DI MAX MEAN ' DI
plants plants
631 P1468378 1 16 86.8 3 100% 16.6 25 3 100%
632 PI468408A 88 42.7 2 88% 8.2 14 2 100%
633 P14684088 140 49.5 4 100% 10.2 15 3 100%
634 P1468903 48 49.0 5 90% 16.7 38 3 92%
635 P1468907 30 38.3 0 - 6.5 13 3 92%
636 P1468915 20 12.3 3 75% 13.3 17 2 100%
637 P1468920 30 17.3 1 100% 16.0 27 2 100%
638 P146892] 15 16.8 3 100% 15.9 22 1 100%
639 P147022] 89 45.2 3 100% 0.0 0 3 100%
640 P1470222 53 26.2 3 100% 10.5 24 3 92%
641 P1470223 136 116.8 5 100% 15.5 30 3 100%
642 P1470224 1 16 83.3 5 100% 15.2 19 3 100%
643 P1470225 110 57.0 4 88% 15.8 25 3 100%
644 PI470227A 1 16 71.0 4 100% 15.2 25 3 100%
645 P1473573 102 97.3 1 100% 2.4 4 1 75%
646 P1475829A 81 76.0 5 75% 1 1.5 19 3 100%
647 P1479709 89 78.8 2 88% 13.5 24 3 100%
648 P1479710 36 40.5 3 83% 25.3 38 3 100%
649 P147971] 71 27.2 4 75% 20.8 32 3 25%
650 P1479712 20 25.7 4 100% 10.9 20 3 100%
651 P1479713 88 41.3 3 83% ' 12.2 23 2 100%
652 P1479714 110 102.8 4 100% 15.8 23 3 83%
653 81479717 28 26.5 4 94% 16.0 31 2 100%
654 PI479718A 85 62.5 4 81% 17.0 23 3 100%
655 P14797188 26 15.2 4 94% 8.8 14 3 100%
656 P1479719 115 82.0 1 100% 8.7 22 3 100%
657 P147972] 97 73.8 5 100% 5.9 22 3 100%
658 P1479723 102 78.2 4 94% 23.9 48 3 100%
659 P1479724A 104 92.3 3 92% 17.1 31 3 100%
660 P14797248 78 76.7 3 100% 10.2 20 2 100%
661 P1479725A 160 133.7 4 94% 6.5 1 1 3 100%
662 P14797258 1 12 108.2 4 100% 18.8 30 3 92%
663 P1479726 - - 5 100% 10.7 60 3 92%
664 P1479727 44 78.2 1 100% 28.6 41 3 75%
665 PI479728A 109 92.0 4 100% 7.7 16 3 83%
666 P14797288 196 55 .0 2 100% 9. 1 18 3 100%
667 P147973] 106 84.7 5 90% 12.8 32 3 92%
668 P1479732 175 18.7 2 100% 15.3 28 3 83%
669 P1479734 150 91.0 4 100% 8.8 18 2 88%
670 P1479736 6 3.3 2 100% 15.8 22 3 100%
671 P1479737 66 29.3 5 100% 13.5 29 3 100%
672 P1479738 1 10 65.8 2 100% 1.3 3 3 100%
673 P1479742 150 36.8 2 88% 5.9 1 l 3 92%
674 P1479743 78 29.8 4 100% 4.0 8 3 92%
675 P1479758 71 34.0 3 100% 0.0 0 3 25%

 

74

 

 

 

Replication] Replication2
Entry PU FC N f N f
' number MAX MEAN °' 0 D1 MAX MEAN °' ° DI
plants plants

676 P1479759 78 54.8 3 100% 6.6 18 3 100%
677 P1479762 36 32.8 4 100% 7.6 14 3 100%
678 P1483459 124 32.2 4 75% 11.6 18 3 100%
679 P1491548 150 58.7 4 100% 12.8 21 3 100%
680 P1491579 31 16.0 4 100% 9.9 19 3 100%
681 P1503333 66 50.0 3 100% 0.0 0 3 100%
682 P1503337 - - 0 - - - O -
683 P1503338 66 43.0 1 100% 0.0 O 3 100%
684 P1503340 26 33 .3 5 100% 17.3 34 3 92%
685 P1518703 9 9.0 5 100% 10.7 23 2 100%
686 P1518706C 32 16.5 3 100% 3.5 12 2 88%
687 P1518707 64 31.4 5 100% 6.3 18 3 100%
688 P1518708 43 29.2 1 100% 2.6 9 3 100%
689 P1518709 36 14.7 3 100% 5.2 12 3 92%
690 P1518710 20 7 .0 4 88% 6.9 18 3 100%
69] P151871] 29 19.5 3 75% 0.0 0 3 100%
692 P1518712 17 19.3 2 100% 11.7 32 2 100%
693 P1518718A 15 9.5 3 100% 7.8 18 3 92%
694 P15187188 15 6.0 4 100% 22.5 55 3 92%
695 P1532434 100 6.3 5 100% 7.3 18 3 100%
696 P1532439 38 21 .0 2 88% 1 .2 3 3 100%
697 P1532441A 150 33.6 4 100% 2.1 6 3 100%
698 P15324418 22 13.7 4 100% 6.8 16 3 100%
699 P1532443 60 30.8 3 83% 17.9 32 3 100%
700 P1532444A 200 99.0 3 92% 6.9 18 3 100%
701 P15324448 200 34.0 4 100% 0.0 0 3 25%
702 P1532447 182 118.2 5 95% 13.1 18 3 100%
703 P1532464 250 - 5 100% 2.4 7 3 100%
704 P1532465 200 40.0 4 100% 20.9 50 3 100%
705 P1538387 100 51 .0 4 100% 15.5 21 3 100%
706 P1538387 100 78.0 5 100% 21.6 50 3 100%
707 P1538394 150 59.0 4 100% 0.9 4 2 100%
708 P1538400 150 100.0 4 100% 19.0 32 2 100%
709 P15490418 0 29.8 2 75% 0.0 0 2 75%
710 P15490428 - - 5 75% 6.7 12 2 75%
7]] P1561230 110 76.7 3 100% 10.5 18 2 100%
712 P15612338 88 75.3 4 100% 5.4 16 3 100%
713 P1561233C 103 28.2 4 100% 5.8 11 3 100%
714 P1561240 22 14.3 1 100% 15.8 18 1 100%
715 P1561284 - - 0 - 0.0 0 2 100%
716 P1561285A 89 95.0 3 100% 0.0 0 3 100%
717 P1561307 108 66.2 4 100% 6.5 12 3 92%
71.8 P1561314A 93 45.7 2 100% 0.0 0 1 25%
719 P1561326 - 166.0 1 100% 20.3 50 3 100%
720 P1561327A 24 63.0 . 3 100% 1.3 4 3 100%

 

75

 

 

 

Replicationl Replication2
Entry PU FC N0 of No of
number MAX MEAN ' D1 MAX MEAN ‘ DI
plants plants
72] P15613278 89 82.3 5 95% 12.0 23 3 100%
722 81561327C 18 51.3 4 94% 13 .6 21 3 100%
723 815613278 55 25.8 5 100% 18.2 36 3 100%
724 81561335 173 72.0 2 88% 17.5 28 3 100%
725 P1561353 36 11.5 5 100% 18.6 30 3 100%
726 P1561354 1 16 74.2 2 88% 9.5 30 2 100%
727 P1561367 33 67.8 3 100% 4.5 18 3 25%
728 P15671568 117 91.0 1 100% 23.3 30 2 88%
729 81567159A 1 12 34.7 4 100% 22.5 32 3 100%
730 P15671598 103 100.7 3 100% 41.4 52 3 100%
731 P1567160 0 0.3 5 100% 15.1 22 2 100%
732 81567161 115 153.8 3 100% 8.2 16 3 100%
733 P1567162 66 71.7 3 100% 14.6 33 3 100%
734 81567166 260 231.8 3 100% 16.3 28 3 100%
735 81567168 126 194.8 4 100% 11.0 18 3 100%
736 81567169 45 22.3 2 100% 1.0 6 2 100%
737 81567170A 50 25.3 4 100% 11.7 20 3 100%
738 815671708 22 13.0 3 100% 15.9 25 3 100%
739 81567175C 130 117.3 1 100% 15.3 18 l 100%
740 81567288A 95 76.5 4 100% 5.3 10 3 100%
741 81567293 1 15 69.5 3 100% 3.6 12 3 100%
742 81567313 170 1 12.8 3 92% 6.5 10 3 92%
743 81567323A 130 90.5 2 100% 12.4 23 3 100%
744 815673238 90 82.3 1 100% 9.2 16 3 100%
745 81567323C 124 107.7 1 100% 12.7 36 3 100%
746 815673254 48 69.5 5 95% 9.9 18 2 100%
747 81567351A 17 12.8 4 94% 12.0 20 3 92%
748 815674804 82 51.8 4 100% 1 .7 10 2 88%
749 815674808 52 41.0 1 75% 10.2 18 2 100%
750 8156748] 132 125.7 2 100% 13.1 25 2 100%
751 81567485 77 53.7 3 92% 15.1 32 2 100%
752 8156748614 112 108.3 2 100% 13.9 15 1 100%
753 815674868 160 114.8 4 88% 0.0 0 3 25%
754 81567504 62 52.2 3 100% 22.8 58 2 100%
755 81567505 161 168.0 3 92% 24.9 30 3 100%
756 81567507A 51 38.7 1 100% 12.4 14 l 100%
757 815675078 32 16.8 4 94% 15.7 20 1 50%
758 81567512A 14 10.7 4 94% 12.3 23 2 75%
759 815675128 106 59.0 4 88% 18.3 30 3 83%
760 81567515 17 7.5 3 75% 15.7 20 2 88%
761 81567516A 48 21.2 4 88% 14.0 28 3 75%
762 81567519 34 25.2 3 92% 16.2 31 2 75%
763 815675204 13 1 1 .7 3 67% 0.0 0 3 25%
764 815675208 8 3.3 5 55% 16.9 28 3 25%
765 81567525 100 37.8 3 83% 22.8 30 2 63%

 

76

 

 

Replicationl Replication2

 

 

Entry 81/ FC N f N f
number MAX MEAN °' ° 81 MAX MEAN °' ° DI
plants plants
766 P1567526 124 63.3 2 88% 18.8 22 1 75%
767 81567528A 250 - 2 75% 18.8 22 1 50%
768 P15675288 130 81.0 1 50% 23.8 31 2 75%
769 P1567529 200 81.0 1 75% 13.4 26 2 63%
770 P1567537 8 3.3 3 33% 1.1 2 3 25%
771 81567538A 0 0.0 5 45% 0.0 0 3 33%
772 P15675388 200 65.0 1 100% 2.4 5 3 58%
773 P1567539 250 - 1 100% 1.7 3 3 83%
774 81567541A - - 0 - 24.2 30 3 92%
775 815675418 35 18.2 2 25% 9.8 18 3 25%
776 81567541C 200 - 2 100% 26.5 32 3 75%
777 81567543A 1 2.8 4 31% 1 1.2 16 2 63%
778 P15675438 250 - 4 94% 16.4 22 2 88%
779 P1567543C 4 1.0 3 25% 0.0 0 1 25%
780 P1567545 31 21.5 3 58% 0.6 1 1 25%
781 P1567547 32 31.5 5 100% 5.6 3 33%
782 81567549A 132 96.0 2 75% 18.6 30 2 75%
783 P15675498 250 - 4 88% 14.6 22 2 63%
784 P1567550 200 - 4 75% 8.5 18 2 63%
785 P1567558 109 57.7 2 75% 13.1 16 1 50%
786 P1567559 200 101.7 2 75% 13.3 20 3 58%
787 P1567560 3 1.2 4 63% 0.0 0 1 50%
788 81567563A 103 46.0 5 80% 12.1 20 3 83%
789 P15675638 21 10.7 3 75% 25.2 35 2 100%
790 81567574A 200 1 1 1.2 3 100% 13.1 19 3 100%
791 P15675748 200 - 4 81% 15.7 18 2 88%
792 P1567576 300 - 1 75% 16.1 20 3 92%
793 81567583A 250 - 4 94% 27.1 33 1 100%
794 815675838 250 - 2 88% 11.2 18 3 75%
795 P1567584 1 0.3 3 83% 0.0 0 3 25%
796 81567585A 2 1.2 3 25% 5.3 10 3 33%
797 P15675858 200 - 2 88% 4.3 8 1 75%
798 81567586A 200 - 4 94% 24.1 30 3 83%
799 P15675868 200 - 2 75% 12.3 20 3 92%
800 P1567589 80 65.7 1 75% 19.3 28 2 100%
801 81567590A 200 - 1 100% 21.9 30 3 100%
802 P1567592 10 4.7 4 31% 2.1 6 3 25%
803 PI567593A 16 8.0 2 38% 0.1 1 3 25%
804 81567594A 200 175.0 4 94% 17.8 24 2 63%
805 P15675948 120 59.3 2 63% 3.1 6 3 25%
806 P1567595A 250 250.0 2 75% 8.8 12 2 100%
807 P15675958 200 33.3 2 75% 10.4 18 3 100%
808 815675974 1 12 89.8 3 100% 13.1 22 2 100%
809 P15675978 6 3.7 3 42% 5.9 1 1 3 25%
810 P1567597C 2 0.7 2 25% 0.0 0 2 25%

 

77

 

 

 

Replication] Replication2
Entry PI] FC N f N I
number MAX MEAN °' ° DI MAX MEAN °' ° DI
plants plants

81 1 81567598A 72 58.7 3 100% 4.5 8 2 75%
812 815675988 3 1.5 3 25% 0.0 0 3 33%
813 81567599 96 71.3 1 25% 7.9 14 3 67%
814 8156760] 8 2.7 4 44% 3.0 6 2 25%
815 815676024 80 59.8 2 75% 15.6 26 g 2 75%
816 815676028 100 62.0 2 88% 20.8 28 1 75%
817 81567603A 14 8.8 2 25% 7.7 12 3 50%
818 81567607A 70 62.7 0 - 17.8 30 3 58%
819 81567609 219 168.3 2 88% 9.3 15 2 63%
820 81574478A 139 186.0 3 100% 0.6 5 3 92%
82] 815744788 98 52.3 3 92% 19.6 28 3 67%
822 81574478C 184 129.5 5 100% 13 .2 21 3 92%
823 81574479A 144 106.5 2 100% 17.5 24 3 67%
824 815744798 72 60.0 1 100% 14.6 24 2 50%
825 81574480A 212 143.0 2 100% 15.8 24 3 75%
826 81574481 140 128.2 2 100% 0.0 0 3 25%
827 81578368 82 50.2 2 88% 33.1 56 3 75%
828 81578380A 90 100.7 4 94% 19.0 25 2 88%
829 81578382 56 54.0 3 100% 19.3 32 2 100%
830 81578492 200 85.3 3 92% 24.0 30 3 92%
83 1 81578493 - - 0 - 24.8 42 3 100%
832 PIS78498A 20 10.7 2 38% 6.9 12 3 100%
833 81578500 200 56.3 3 92% 8.1 17 2 100%
834 81578502 2 25.3 2 88% - - 0 -
835 81578503 3 51.0 2 100% 10.3 15 3 100%
836 81578505 80 93.7 4 94% 1 1.3 22 1 75%
837 8159292] 161 130.3 4 100% 13.7 21 3 92%
838 81592926 82 94.2 3 100% 12.2 25 3 100%
839 81592931 52 27.3 4 100% 26.0 40 3 33%
840 81592932 125 97.5 5 100% 12.1 . 15 3 75%
841 81592933 200 166.7 4 94% 16.8 23 3 100%
842 81592936 120 88.0 4 100% 21.9 31 3 100%
843 81592941 93 81.7 3 100% 10.0 18 3 100%
844 81592942 200 - 1 100% 15.0 21 3 100%
845 81592943 194 181.2 4 94% 13 .2 17 2 100%
846 81592944 30 39.0 5 100% 9.5 18 3 100%
847 81592957 - - 0 - 9.0 30 2 100%
848 81592958 120 100.7 1 100% 7.0 16 3 75%
849 81592959 101 82.0 1 100% 1 1 .4 1 8 2 63%
850 81592960 90 81.5 4 94% - - 0 -
851 81592967 - - 0 - 10.8 18 2 88%
852 81592968 101 49.5 2 100% 11.0 15 1 100%
853 81592969 52 41.7 1 100% 19.0 30 2 88%
854 81592970 90 63.8 3 100% 8.8 22 3 67%
855 81592972 51 24.3 2 100% - - 0 -

 

78

 

 

 

Replicationl Replication2
Entry PU FC N 1 N 1
number MAX MEAN °' ° D1 MAX MEAN °‘ ° D1
plants plants

856 81592973 - - 0 - 17.2 26 1 75%
857 81592977 96 77.0 3 100% 0.3 1 2 25%
858 81592978 - - O - 0.0 0 1 100%
859 81592979 5 7 55.7 3 100% 4.1 6 1 100%
860 81593942 162 101.3 5 95% 7.0 10 3 100%
861 81593943 17 13.3 1 75% 9.3 22 3 100%
862 81593953 120 104.2 5 100% 0.5 1 3 100%
863 81593958 43 40.5 5 100% 1 .5 4 3 100%
864 81593959 130 86.5 5 95% 3.0 6 3 100%
865 81593960 143 108.0 4 94% 10.8 20 3 100%
866 81597415 28 63.5 5 100% 6.4 15 3 67%
867 81597419 105 141.8 5 90% 0.0 0 1 75%
868 . 81597420 54 70.2 2 100% 15.7 20 l 100%
869 81597421 106 83.2 5 100% 1 1.1 14 3 50%
870 81597427A 122 98.7 5 100% 6.0 15 3 100%
87] 815974278 141 96.3 5 95% 8.3 16 3 100%
872 81597429 153 107.7 2 100% 9.7 18 3 100%
873 81597432 67 91.5 3 92% 17.3 31 3 100%
874 81597439 167 106.8 5 95% 16.8 38 3 50%
875 81597440A 106 98.7 3 92% 13 .8 l8 3 83%
876 815974408 259 224.2 4 100% 1 1.3 16 3 58%
877 81597440C 82 7 1.8 5 90% 1 1.0 21 2 100%
878 81597441 1 15 99.7 5 100% 3.5 8 2 100%
879 81597442 1 17 94.8 5 100% 23.6 38 3 67%
880 81597443 126 120.3 5 100% 6.9 12 1 100%
881 81597444 183 147.3 5 100% 2.3 4 2 100%
882 81597445 60 75.2 4 100% 16.7 27 2 100%
883 81597446 69 48.2 4 100% 2.2 10 2 88%
884 81597467 58 66.7 4 100% 17.8 32 3 100%
885 81602490 88 64.3 4 100% 2.4 6 2 100%
886 PI603293A 87 85.3 3 100% - - O -
887 816032938 133 123.3 3 100% 0.0 0 3 58%
888 81603298 177 120.2 5 95% 12.7 26 2 100%
889 81603302 178 127.3 4 88% 15 .9 17 1 100%
890 81603303 194 140.0 3 100% 14.8 24 2 63%
891 81603304 1 12 89.2 3 92% 14.3 18 3 100%
892 81603305 68 96.0 4 94% 6.3 9 3 100%
893 81603307 1 58 93 .0 3 100% 1 .6 8 3 100%
894 81603308A 142 86.0 5 85% 8.8 15 3 100%
895 816033088 93 95.3 5 90% 9.0 14 3 100%
896 81603309 108 65.0 2 88% 8.8 27 3 100%
897 81603310 42 56.7 4 100% 4.5 9 3 83%
898 8160331] 20 70.7 5 95% 16.0 21 3 100%
899 81603312 161 59.2 2 75% - - 0 -
900 81603315 10 14.3 5 85% 30.5 41 2 88%

 

79

 

 

 

Replication] Replication2
Entry PI/ FC
number MAX MEAN N°' ”f DI MAX MEAN N°' ”f DI
plants plants
901 81603321 97 72.5 5 85% 14.0 28 3 83%
902 P1603323 3 8 36.3 5 90% 1 8 .7 23 2 100%
903 816033248 71 93 .3 4 94% 9.7 18 3 92%
904 81603325 128 88.0 4 88% 33 .0 41 1 100%
905 81603327 106 98.7 1 100% 16.6 25 2 100%
906 81603329 201 157.3 3 92% 1 1.1 21 3 100%
907 81603330 1 80 1 10.2 3 100% l .7 3 l 100%
908 81603331 101 55 .3 4 94% 14.1 26 2 88%
909 81603332 1 3 1 102.8 4 1 00% - - 3 100%
910 81603333 - - 0 - 1 1 .0 l 6 2 100%
911 816033358 106 88.2 3 83% 12.9 18 1 100%
912 81603336 32 48.2 5 100% 6.1 8 l 100%
913 PI603337A 35 19.5 5 90% 4.4 12 3 100%
914 816033378 4 4.2 4 63% 8.3 18 3 100%
915 81603338 111 106.0 5 95% 17.1 23 1 100%
916 816033398 131 102.8 4 88% 5 .0 8 3 67%
917 81603340 112 84.7 4 81% 3.1 6 1 100%
918 81603341 162 139.2 5 100% 6.7 16 3 75%
919 81603342 101 58.8 3 92% 4.2 8 2 63%
920 PI603343A 224 150.5 4 75% 6.3 12 3 67%
921 81603344 80 102.8 3 100% 5.3 13 2 75%
922 81603345 90 82.3 1 100% 7.7 12 l 100%
923 81603347 141 108.8 4 94% 16.6 21 2 75%
924 PI603348A 170 136.0 1 100% 12.6 23 2 100%
925 816033488 180 141.8 4 100% 25.3 32 1 100%
926 PI603348C 65 59.7 4 94% 9. 1 13 3 100%
927 81603349 210 133.0 2 100% 5 .3 8 2 100%
928 81603350 60 35.5 3 100% 4.4 6 1 75%
929 81603351 205 101.2 3 92% 3 .3 6 3 67%
930 81603352 1 12 67.3 4 100% 5 .6 8 1 100%
931 81603353 171 157.7 1 100% 12.9 28 3 67%
932 81603354 165 100.2 3 100% 23 .3 36 2 100%
933 81603356 92 48.7 5 100% l 1.5 16 3 100%
934 81603357 139 92.5 4 100% 5 .8 12 2 100%
935 PI603358A 161 133.0 1 100% 4.7 6 1 100%
936 P16033588 43 31.3 3 92% 1 1.2 18 3 100%
937 81603359 51 43 .5 2 100% 2.9 5 1 50%
938 81603360 141 74.8 3 100% 4.4 1 8 2 100%
939 81603361 92 76.7 5 90% 9.0 18 1 100%
940 81603362 1 1 1 55 .8 2 88% 17.8 29 2 100%
941 PI603363A 74 37.7 3 92% 20.3 32 3 92%
942 816033638 62 44 .5 3 100% 15.7 20 3 83%
943 81603364 83 62 .0 l 100% 4.6 l 8 2 100%
944 81603365 43 30.5 3 100% 6.6 14 3 83%
945 81603367 92 78 .7 3 100% 19. 8 3 5 l 100%

 

80

 

 

 

- Replicationl Replication2
Entry PI, FC N f N 1
number MAX MEAN °' ° DI MAX MEAN °° ° DI
plants plants

946 81603368 140 63.8 4 100% 9.5 16 3 100%
947 81603369 73 59.2 4 94% 10.9 18 3 100%
948 81603370 68 37.3 5 100% 15.8 30 1 100%
949 81603371 36 28.3 1 75% 0.0 0 1 75%
950 81603372 136 109.3 3 100% 22.7 35 2 100%
951 81603373 96 48.0 2 88% 14.2 19 1 100%
952 81603374 144 92.0 4 88% 3.2 8 2 88%
953 81603375 79 57.7 4 100% 19.3 36 3 100%
954 81603376 23 42.2 4 94% 6.3 13 3 100%
955 81603377 15 25 .7 5 100% 3.7 8 3 100%
956 81603378A 98 74.0 1 100% - - 0 -
957 816033788 18 23.7 3 100% 7.1 14 3 100%
958 81603380 82 49.0 1 75% 24.8 32 3 92%
959 81603381A 92 64.8 4 94% 3.6 10 3 100%
960 816033818 102 77.8 4 100% 15 .9 26 3 100%
961 81603381C 62 68.2 2 100% 11.3 36 2 100%
962 81603382A 49 36.2 4 100% 17.3 21 1 100%
963 816033828 33 36.5 4 100% 12.5 20 2 100%
964 81603383 90 65.3 5 100% 9.8 18 3 92%
965 81603385 123 107.2 4 94% 0. 1 1 3 100%
966 81603386 98 50.8 5 95% 6.0 12 1 100%
967 81603387 50 58.3 3 100% 14.5 21 3 100%
968 81603389 95 70.8 4 100% 12.5 22 l 75%
969 81603390A 100 102.0 4 100% 17.8 32 3 100%
970 81603391 100 61.8 3 100% 12.4 20 2 100%
971 81603398A 150 90.7 5 95% 7.8 12 2 100%
972 81603399 56 54.7 4 100% 6.3 13 3 92%
973 81603403 131 1 11.0 5 90% l 1.9 26 2 75%
974 816034054 83 54.5 4 94% 10.0 21 2 75%
975 81603412A 45 36.5 2 88% 13.8 20 3 92%
976 816034128 18 32.3 3 92% 11.0 21 2 75%
977 81603414 74 83.8 3 92% 26.2 31 1 100%
978 816034194 72 70.8 3 100% 26.2 42 3 92%
979 81603420 70 73.8 4 94% 13.3 21 3 100%
980 81603421A 90 76.5 5 95% 11.0 20 3 75%
981 81603422A 10 6.7 4 88% 6.8 18 2 88%
982 81603425 43 39.0 5 100% 25.3 32 2 75%
983 81603426G 84 43.0 4 100% 17.1 30 3 100%
984 816034288 22 12.0 3 92% 3.3 8 3 100%
985 81603429D 3 1 41.2 3 92% 9.3 18 3 100%
986 81603430A 38 49.7 4 94% 4.1 10 3 100%
987 81603433A 26 28.8 4 100% 5.4 20 3 100%
988 816034338 222 115.5 3 100% 18.4 30 3 100%
989 81603434 93 88.8 5 100% 13.7 22 3 100%
990 81603436A 210 123.2 3 92% 12.8 20 2 100%

 

81

 

 

 

Replicationl Replication2
Entry 81/ FC
number MAX MEAN M ”f D1 MAX MEAN N°' °f In
plants plants

991 816034368 149 120.7 5 90% 8.8 30 2 88%
992 81603438C 15 24.3 4 100% 9.4 22 3 100%
993 81603438D 40 60.8 4 88% 7.8 12 2 100%
994 81603441 222 66.3 5 95% 8.1 20 3 75%
995 81603443C 75 51.0 5 85% 5.1 12 2 75%
996 81603444A 124 52.5 3 83% 10.2 19 2 75%
997 81603444C 170 114.0 4 81% 8.4 15 2 100%
998 81603446 156 125.0 5 90% 13.7 20 2 100%
999 81603451A 66 51 .7 3 92% 4.3 20 2 100%
1000 81603452 33 48.3 4 88% 9.8 15 3 100%
1001 81603454 68 26.7 4 94% 10.6 16 3 100%
1002 81603455A 17 26.3 5 100% 34.9 45 3 100%
1003 81603456 13 5.3 3 75% 3.1 6 1 100%
1004 81603463 10 7.8 5 95% 12.9 23 3 100%
1005 81603464 42 17.8 3 100% 0.8 2 3 100%
1006 816034654 101 86.0 4 94% l 1.3 15 3 100%
1007 81603467 163 126.3 4 94% 15.7 41 3 100%
1008 81603470 164 121.8 3 92% 0.0 0 1 100%
1009 81603471 164 125.7 3 92% 13.9 26 3 92%
1010 81603472A 21 11.3 3 83% 2.8 6 1 75%
1011 81603473 14 38.0 3 92% 5.6 12 3 67%
1012 81603474 47 37.8 3 83% 9.0 20 3 42%
1013 81603475 43 35.8 5 90% 24.8 35 2 88%
1014 81603477A 56 98.0 1 75% 34.9 45 1 50%
1015 816034778 165 169.5 3 100% 25.4 56 3 67%
1016 81603479 111 110.8 5 90% 12.2 18 2 88%
1017 81603480 9 16.8 4 94% 21.4 42 3 75%
1018 81603481 104 60.8 3 83% 21.6 38 3 92%
1019 81603482 42 55.3 4 88% 4.9 10 2 63%
1020 81603483 198 139.7 4 88% 15.0 27 3 75%
1021 81603484 135 120.7 4 94% 17.5 30 3 75%
1022 81603485 152 140.8 3 100% 14.0 18 1 75%
1023 81603486 163 151.3 4 88% 19.1 38 3 75%
1024 81603488 127 103.7 2 100% 10.0 18 3 100%
1025 81603497 83 89.3 3 92% 33.5 48 3 75%
1026 81603602 177 100.7 4 88% 39.4 62 2 63%
1027 81603612 35 21.3 3 92% 9.0 13 2 75%
1028 81603613 186 70.2 2 88% 19.0 40 2 75%
1029 81612708D 90 78.8 5 100% 9.9 12 2 88%
1030 81612709C 43 44.2 4 94% 0.0 0 l 50%
1031 81612713A 34 38.0 3 100% 9.2 16 2 63%
1032 816127138 36 34.7 3 100% 0.0 0 1 75%
1033 81612714A 139 119.5 5 100% 0.5 1 3 67%
1034 816127148 123 93.7 5 100% - - 0 . -
1035 81612715 104 89.0 5 100% 18.6 30 2 63%

 

82

 

Replicationl Replication2

 

 

Entry “I FC N f N 1'
number MAX MEAN °° ° 1)] MAX MEAN ”' ° DI
plants plants
1036 81612716 97 87.0 5 100% 11.0 15 2 63%
1037 81612717 154 106.0 4 100% 13.3 36 2 63%
1038 81612722 117 114.8 4 94% 14.8 26 3 75%
1039 81612723 149 100.5 2 88% 21.4 32 2 100%
1040 81612724A 65 39.7 5 95% 19.3 26 3 75%
1041 81612725 68 51.5 4 100% - - l 25%
1042 81612726 139 121.3 3 92% 26.0 38 2 88%
1043 81 549020 111 63.5 3 75% - - 1 25%
1044 Resist.chk 6 3.2 5 55% 0.0 0 1 25%
1045 Suscep.chk 128 121.5 3 67% -

 

81: Plant Introduction, MG: Maturity Group, MAX: Maximum Number of Aphids per Leaflet
D1: Damaging Index, Mean: Average Number of Aphids on Three Leaflets
Resist.chk: Resistant Check, Snscep.chk: Susceptible Check

 

83

 

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