meats 2M4 6¢<&SZ?> Date 0-7 639 IJIHRAJCY Michigan State University This is to certify that the thesis entitled DETERMINING THE S-DABILITY OF VARIOUS FIBER TYPES presented by Sarah Elizabeth Walbridge has been accepted towards fulfillment of the requirements for M. 8. degree in mm Justice on )M ‘/,Major pldfessor Max/03 MS U is an Affirmative Action/Equal Opportunity Institution PLACE IN RETURN BOX to remove this checkout from your record. TO AVOID FINES return on or before date due. MAY BE RECALLED with earlier due date if requested. DATE DUE DATE DUE DATE DUE 6/01 c:/CIRC/DateDue.p65-p.15 DETERMINING THE SHEDDABILITY OF VARIOUS FIBER TYPES By Sarah Elizabeth Walbridge A THESIS Submitted to Michigan State University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE School of Criminal Justice 2003 ABSTRACT DETERMINING THE SHEDDABILITY OF VARIOUS FIBER TYPES By Sarah Elizabeth Walbridge The shedding quality of a fabric has utility in forensic science. Fibers are frequently recovered as evidence in criminal cases. The application of the shedding potential of a garment is thus significant. A standard method for determining the sheddability index of various fiber types was developed. The low-adhesive backing of a 3” x 3” 3M© post-it note was utilized to simulate a ‘natural’ shedding of the fabric. A raw mean shedding index was assigned for the thirty—one fabrics tested by this method. Based on this data, fabrics were categorized from high to low shedders. Fiber-transfer studies with nineteen of the tested fabrics as donors and white/black felt as recipients was performed and compared to the previously determined shedding index categories. Two simulated contacts, a handshake (no contact), and a hug (friendly contact) were used as transfer conditions. The hug transfer was repeated three times to calculate a mean. Overall, the method developed for determining the sheddability of a fabric is efficient, reproducible, and reliable. The post—it note method results correlated well with the shedding potential. The rank correlation coefficient (R:0.753) indicates that Raw Mean Shedding Index and Mean Transfer are positively correlated. This supports the hypothesis that determining the sheddability of a fabric is a useful measurement for evaluating fiber transfer. ACKNOWLEDGMENTS The author wishes to thank all those who have encouraged and supported the success of this research project. The author owes a large thank you to Dr. Jay Siege] for the encouragement, patience, and guidance through this project and many occasions beyond. The author is equally grateful to Max Houck for his assistance, feedback, and ideas which were constantly requested and always thoughtfully provided. The author is grateful to Rick Myers for participating in the transfer study. The author also extends a thank you to her family who has waited patiently for the completion of this research project with continuing support. Lastly, thank you Chris for the many Sundays of unassisted laundry duties. iii TABLE OF CONTENTS List of Tables ......................................................................................... vi List of Figures ....................................................................................... vii Chapter 1 Introduction ............................................................................................ 1 Chapter 2 Literature Review ..................................................................................... 7 Transfer and Persistence .......................................................................... 7 Secondary Transfer ............................................................................... 10 Fabric Sheddability ............................................................................... l 1 Hypothesis of Current Study .................................................................... 13 Chapter 3 Methods and Materials ............................................................................... 15 Fabrics Chosen for this Research ................................................................. 15 Materials Utilized for Determining Shedding Potential ....................................... 16 Method Utilized for Determining Shedding Potential ......................................... 17 Materials Utilized for Transfer Studies .......................................................... 19 Method Utilized for Transfer Studies ............................................................ 19 Handshake Transfer ................................................................................ 20 Hug Transfer ........................................................................................ 20 Materials and Method Used for Removing Transferred Fibers .............................. 21 Statistical Methods Used .......................................................................... 22 Chapter 4 Results ................................................................................................ 25 Intra-comparison of Raw Mean Shedding Index ............................................ 25 Fabric Structure and its Effect on Shedding .................................................. 26 A Fiber-Transfer Study ......................................................................... 27 The Hug Transfer ................................................................................ 29 Results of Spearman R Computation ......................................................... 3] Repeated Hug Contacts ......................................................................... 31 Chapter 5 Conclusions .......................................................................................... 32 The Significance of a Sheddability Index in the Laboratory and the Courtroom.......38 Chapter 6 Future Research ..................................................................................... 43 TABLE OF CONTENTS, continued Appendices ........................................................................................... 45 Appendix I Fabric Descriptions ................................................................................. 45 Appendix 11 Fabric Weave Counts .............................................................................. 46 Appendix [II Summary of Raw Mean Shedding Index Calculations ......................................... 47 Appendix IV Comparison of Raw Mean Shedding Index between the Same Fabric Types .............. 49 Appendix V Categorization of Nineteen Fabrics Tested ...................................................... 52 Appendix VI Results of Hug and Handshake Transfers ........................................................ 53 Appendix VII Calculation of Mean Transfer ..................................................................... 55 Appendix V111 Results of MINITAB Statistical Program ........................................................ 56 References ............................................................................................ 57 LIST OF TABLES Table 1 Example of Shedding Data for a Woven and Knit Fabric ..................................... 25 Table 2 Number of Fibers Transferred during Hug(s) and Handshake ................................ 29 vi LIST OF FIGURES Figurel Photograph of Materials Needed for Determining Shedding Potential... ......................... 17 Figure 2 Raw Mean Shedding Index Comparison of Eight Polyester Fabrics Tested ................ 26 Figure 3 Plot of the Affect of Fabric Structure on Shedding ............................................. 27 Figure 4 Data Yielding Five Shedding Categories ......................................................... 28 Figure 5 Ratio of Raw Mean Shedding Index to Mean Hug Transfer ................................. 3O INTRODUCTION The purpose of this research was to develop a standard method for determining the sheddability of a fabric that is efficient, reproducible, and realistic to real-world conditions. More importantly this research investigated whether there is a useful relationship between raw mean shedding index and transfer rate. The raw mean shedding index of a fabric type will assist the examiner in determining the significance of the fiber evidence whether it is a single fiber or a tuft of many fibers. The presence or absence of fiber evidence can have considerable influence on linking a suspect to a crime scene or particular location. As the mass production of fabrics and garments continues and as we continue to come in contact with each other, the opportunity for transfer increases. Understanding how and why fibers are present in criminal and civil circumstances is essential for a scientist to analyze and interpret the probative value of the evidence at hand. Criminal investigators and forensic examiners should not underestimate the evidential value of fibers. Fiber evidence speaks volumes to the environment, surroundings, and circumstances that a person or object may have been in at a given time. Edmond Locard was the first to recognize the significance of evidence transfer. Locard established what is now known as his exchange principle [10]: contact between two objects always results in transfer of some material. This remains a central principle of the analysis of scientific evidence. When applying this principle to fiber evidence, three components must be considered: shedding, transfer and persistence. Fiber transfer has been generally defined as the release or shedding of a fiber from one garment or textile onto either another textile or non-fiber material. Fiber shedding can be defined as a type of transfer. Research has shown that the dominant method of transfer is mechanical [18]. Factors involved in how easily a fiber transfers include the length; cross-sectional shape; weave type of the donor garment; age and environmental effects; the force and duration of the contact; and the number of contacts; as well as the characteristics of the recipient garment [l3]. Logically, one would expect short loose fine fibers from older garments to transfer more readily than fibers from tightly woven newer garments. Pressure and contact are the main components necessary for a fiber transfer to occur [14]. However, how the contact and pressure are involved in a fiber transfer may vary. For example, an individual may be wearing a highly sheddable shirt made of loose, acrylic fibers. This individual’s shirt could shed fibers simply from the mechanical forces the individual exerts on the shirt when wearing it. Without any contact, many of these loose fibers could then be transferred to another individual nearby who is wearing a brushed denim shirt-if the two individuals brushed shoulders, for example. If these same two individuals were to hug one another the chances of the loose acrylic fibers being transferred to the brushed denim shirt increase, as does the number of fibers transferred. Therefore, fiber transfer can occur in either situation (direct contact or no direct contact) when two individuals are in the same environment. The number of fibers transferred in either example would probably be relatively high because the donor garment was made from loose acrylic fibers and the recipient garment was made from brushed denim, which is a good recipient for those loose acrylic fibers. A fiber transfer between two garments or textile objects in which a minimal amount of fibers are transferred can also provide information for instance, if the same two individuals in the former example are now wearing a tightly knit polyester shirt (donor) and a nylon jacket (recipient). The tightly knit polyester shirt will not shed fibers as easily as the loosely constructed acrylic shirt. In addition, the nylon jacket has a smooth surface that does not retain shed fibers as well as brushed denim. The significance of finding a single polyester fiber on the nylon jacket may be as informative as finding a cluster of acrylic fibers on the brushed denim shirt. Persistence is the determining factor on whether or not such items as fibers may be found on a person or object after a transfer. Persistence affects the chance of finding a foreign fiber on a recipient after any interval of time has passed since initial transfer. Persistence is affected by such variables as time, activity of wearer, washing, location of transferred fibers, and type of recipient material. Although there are many factors to consider after a transfer has occurred, the shedding potential of a garment must be considered when evaluating both the transfer and persistence of fibers. A loss of fibers after transfer should be expected and this makes interpreting the results of a fiber analysis problematic. If the shedding potential of a textile could be determined, however, the interpretation of the fiber(s) might be clearer. It is important for fiber examiners to compare the shedding ability of one fabric compared to another. Accurately determining the shedding potential of a garment could provide valuable insight into interpreting fiber evidence that may consist of a large number of fibers or only a few fibers. Determining the significance of one fiber can be challenging. There are many variables that affect fiber transfer and having a consistent method for measuring the shedding potential of a garment would be helpful in interpreting fiber evidence. A standard method for determining the shedding potential of a textile could lay the foundation for a uniform interpretation of fiber evidence in forensic casework. Previous research by Coxen, Grieve, and Dunlop [2] has noted the inaccuracy of using Scotch Magic tape for determining the shedding potential of fibers. Therefore, the low-adhesive backing of a 3”x 3” 3M© post—it note was utilized in this research to simulate a ‘natural’ shedding of the fabric. Fabric structures for the tested fabrics included knits and woven fabrics. The fabrics used for this research consisted of a single fiber type (100% content) because forensic examiners always determine the relative amounts of foreign fibers found on a recipient garment by counting sole content fibers regardless of whether it is a fabric blend. Shedding indices for thirty—one different types of fabrics were assigned after four trials were carried out. A Raw Mean Shedding Index (RMSI) value was calculated for all thirty—one fabrics. A fiber—transfer study with the tested fabrics as donors and white/black felt as recipients was performed and compared to the previously determined shedding index. The transfer study was conducted as a means for evaluating the raw mean shedding index data. The classic weighted cigar box transfer method could have been used, as well as many other published transfer methods. However, the author felt that a transfer method that reflected human contact could evaluate a fabrics’shedding potential in a more realistic manner. The purpose of the research was to develop a method for determining a fabrics shedding potential; it was important not only to develop a novel method but to also evaluate and assess the usefulness and significance of that method. With the goal of reflecting human contact, two simulated contacts, a handshake (no contact), and a hug (friendly contact) were used as transfer conditions. To investigate whether the number of transferred fibers decreased after a repeated number of contacts, each hug transfer was repeated three times with each of the tested fabrics using a different piece of recipient felt. Performing the hug transfer three times also provided a calculated Mean Hug transfer that could be compared to the Raw Mean Shedding Index (RMSI). Comparing shedding data from the post-it note method, a type of transfer, and the hug, another type of transfer, was an important aspect of evaluating the developed post-it note method. Utilizing the post-it note method for measuring the shedding potential of a garment would be helpful in interpreting fiber evidence in several different types of fiber cases. For example, a fiber examiner receives evidence from a homicide case. The first item of evidence is a blue acrylic sweatshirt worn by the suspect shooter. The second item of evidence is a black cotton/polyester t-shirt worn by the suspect upon arrest. The examiner uses the post-it note method to determine the shedding potential of the acrylic sweatshirt. Based on several post-it note counts, the examiner determines that the Raw Mean Shedding Index for the sweatshirt is forty—eight (48). This value indicates that the sweatshirt is a high—medium shedder. With this knowledge, the examiner can evaluate the number of fibers transferred on to the suspect’s shirt and determine with assistance of other chemical tests whether the suspect was wearing the blue acrylic sweatshirt at the time of the shooting. The t—shirt is processed for trace evidence by utilizing the taping method and the scraping method. A total of twenty-five blue acrylic fibers are located in the trace evidence collected from the suspect’s t-shirt. This finding indicates that twenty-five fibers were transferred onto the t-shirt from the suspect wearing the sweatshirt and persisted on the t—shirt from the time the suspect took off the sweatshirt to the time they were arrested. By using the post-it note method and determining that the acrylic sweatshirt was a high-medium shedder, the fiber examiner in the above hypothetical case would be able to evaluate the significance of finding twenty-five blue acrylic fibers. The acrylic sweatshirt was predicted to shed a larger amount of fibers. Even though the original amount of transferred fibers is unknown, the shedding potential of the garment assists in predicting and evaluating a fiber transfer. LITERATURE REVIEW Proof of the principle of transfer for fibers became available in 1975 when Pounds and Smalldon [13-15] examined the transfer and persistence of wool and acrylic fibers to various recipient garments. The donor garments were pre-treated with a fluorescent dye except for the acrylic garment, which already contained native fluorescent fibers. Treating the garments with fluorescent dye enabled for easier recovery of transferred fibers. [18] Transfer and Persistence Pounds and Smalldon used a contact method that is very common now in fiber transfer studies. The donor materials were attached to a block and then were pulled, with a constant pressure over a constant distance, across the recipient garment that lay on a flat surface. Their experiments also included repeating the numberof contact passes across the recipient garment for up to eight consecutive contact passes. The fibers were removed manually or by gentle application of adhesive tape between contact passes. From their experiments Pounds and Smalldon [13-14] determined that the transfer and persistence of fibers are significantly affected by pressure of contact, the nature of the recipient garment, the number of repeated contact passes, and fiber length. The experimental data obtained from the work of Pounds and Smalldon provided the scientific community with a solid foundation for future fiber research. Kidd and Robertson [7] researched the transfer of wool, acrylic, cotton, and polyester/viscose fibers to and from clothing typically encountered in criminal cases. The garments chosen for this study were composed of either brightly colored fibers or they possessed inherent fluorescence to allow for easy assessment of fiber transfer. Not only did the authors use a wider range of textile fabrics in their study but they also used a contact method that included hand and machine applied pressure. The pressure of contact was varied and contact passes were repeated. Kidd and Robertson confirmed the major conclusions reached by Pounds and Smalldon. The number of fibers transferred depends on the nature of the recipient fabric with respect to the recipients’ texture. However, when cotton and polyester/viscose donor fabrics were used in the study, the nature of the donor was found to be significant also. The authors compared their applied pressures to a simple experiment with hand pressure and found that there was an obvious increase in the number of fibers being transferred. This concurred with Pounds and Smalldon; however, Kidd and Robertson found that when a maximum or threshold pressure was reached, no additional fibers would transfer. They also agreed with the previous study, that with repeated contacts the number of transferred fibers decreases. Pounds and Smalldon [13] also investigated the persistence of wool and acrylic fibers on various articles of clothing during various times of wear. Their experiments showed that the time of wear was the most important factor in fiber persistence. According to the authors, after an initial rapid loss of fibers there follows a lower loss with a constant low level of fiber persistence extending after time of wear. The rate of fiber decay was the same for the wool and acrylic fibers even though garments differed considerably in surface texture. However, Pounds and Smalldon found that fibers were lost at a significantly higher rate from smooth garments like a cotton laboratory coat. Kidd and Robertson [8] conducted a follow-up study on the persistence of fibers transferred from acrylic, wool and polyester/viscose garments to a variety of recipient garments. They found that fiber persistence was lowered by several factors including: the length of wear, low pressure during initial transfer, and fiber size. Although Kidd and Robertson had used a range of donor and recipient fabric types, the results of their study confirmed the conclusions of Pounds and Smalldon. Their research also presented the importance of other factors on the rate of fiber loss, such as the position of contact and wearing outer garments. Research on fiber shedding was also conducted using blended fabrics. Parybyk and Lokan [12] and Salter et al. [17] conducted similar studies, using blended fabrics as donors. The study conducted by Parybyk and Lokan [12] focused on the difficulties in predicting proportion of transferred fibers based on the manufacturer’s composition label. The fiber composition reported on a manufacturer’s label is calculated as percent by weight. However, the percent by weight is not an accurate indicator of the numbers of shed fibers. The author’s hypothesis was to determine if the proportion of shed fibers on a garment could be predicted by knowing the composition of the original donor garment in terms of the relative numbers of each component fiber. Four bicomponent donor fabrics were used for their research. The simulated transfer was performed with a block wrapped in nylon that was pulled across the donor fabrics. Parybyk and Lokan determined the theoretical and actual ratios of fibers and found that there was good agreement between the theoretical and actual transferred fiber ratios. However, the authors found differences between the percent composition by weight of the fibers and the actual number of fibers transferred. When the theoretical fiber transfer ratio for a garment and the actual transfer ratio differed, the authors noticed that this occurred in garments containing wool. Their observation was consistent with previous research that wool-containing fabrics shed wool to a greater extent than other fibers. Salter, Cook and Jackson [17] performed a similar study with polyester/wool, polyester/viscose and polyester/cotton blends. Cotton and polyester garments were used as recipients during the simulated transfer. Salter et a]. conclusions supported Parybyk and Lokan that the weakest fiber in the mixture (wool and cotton) always shed more fibers and that the percentage content of a fabric does not reflect actual shedding. Salter et al. also found that a rougher recipient material increased the number of fibers transferred from the minor component of the donor blend. Contact pressure also had an effect on the proportion of fibers shed from the minor component. Another related aspect of this research was that the authors used Sellotape (cellophane adhesive tape) as a rough guide to determining which component shed most from a blend. Secondary Transfer The goal of fiber transfer research initially was to assess the factors involved in primary transfer and persistence. Not until Pounds and Smalldon and other researchers built a foundation for fiber transfer was research conducted on secondary and higher order transfers. Grieve, Dunlop and Haddock [4] conducted a classic study on secondary transfer that was inspired by an actual homicide case. The authors recognized that forensic scientists often have difficulties interpreting casework findings because the original number of fibers transferred during an offense is unknown, thus making it difficult to determine the significance of fiber evidence. The authors criticized previous 10 studies for their lack of realism specifically in regards to the size of the donor area being too small and contacts being artificial. According to the authors, extrapolating results in an attempt to fit real life conditions makes the invalid assumption that transfer will be made with equal pressure on all areas of the recipient and that fibers will be equally transferred in every unit area [4]. Due to the nature of a homicide case the authors were working on, they set out to conduct a number of transfer experiments using a red acrylic sweater belonging to the suspect in the case. They investigated primary and secondary transfer and persistence of these fibers onto clothing and seats. The experimental condition of a 15-second struggle kept the number of transferred fibers to a countable level. The authors found that even after a short lS-second struggle, the number of fibers transferred exceeded those transferred by shaking or laying the sweater on a recipient item. They also confirmed previous observations about secondary transfer from clothing and noted that a donor garment such as a high shedding acrylic sweater could transfer a large number of fibers onto a garment worn under the donor, i.e. a shirt. In addition, the authors found that primary-transfer fibers were shown to persist on various seats even after multiple secondary contacts. Fabric Sheddability Coxen, Grieve, and Dunlop [2] researched a method for assessing fabric shedding. The authors’ goal was to develop a method that was easy to execute, reproducible and applicable to real life conditions. Their research design was two-fold. To assess the shedding potential of a fabric, the authors placed a 7.5 x 2.5 cm piece of Scotch Magic tape on the fabric with applied hand pressure. Ten random tapings were taken and a score, ranging from I (very poor shedder) to 5 (very high shedder) was assigned based on the number of target fibers removed. Coxen, Grieve, and Dunlop [2] also conducted a transfer study as a complimentary part to their fabric shedding research. The authors believed that the ability to estimate a garment’s shedding potential would help determine whether that garment was a potential donor of “target” fibers and whether recovered fibers were the product of a primary versus a secondary transfer. The donor garments chosen for their transfer experiments contained only one fiber type. The recipient items were selected to reflect the types of exhibits typically processed in their laboratory. The method used for the transfer experiments utilized a weighted cigar box. The donor garment was placed flat on a bench and a weighted cigar box with a square of the recipient fabric attached to it, was drawn by hand over the donor garment surface. Time,’weight, distance and pressure were fixed for the three transfer trials carried out. Fibers were lifted off recipients by using Scotch Magic 810 tape. The fibers were scanned and counted using a grid under a stereomicroscope. They found that wool donors transfer a high number of fibers but that the taping method tended to over-estimate and under-estimate, depending on the garment, the shedding potential. Cotton donors were influenced by the nature of the recipient fabric but the tape method generally over—estimated the shedding capacity. The acrylic donors tended to be low shedders and the tape method over-estimated their shedding potential. However, the authors found that the taping method gave accurate assessment of shedding ability for the polyester donors. 12 The authors recognized that the method they developed for assessing the shedding potential of fabric does not have practical applications on a routine basis. Coxen, Grieve, and Dunlop [2] concluded their research by suggesting that recourse to their method could yield a more accurate indication of the ability of a fabric to shed fibers. Hypothesis of Current Study The goal of the current research was to develop a method that would give a more accurate indication of a fabric’s shedding potential. The hypothesis to be tested in this research is whether there is a useful relationship between raw mean shedding index and transfer rate. Using the same criteria as Coxen, Grieve, and Dunlop [2], the author intended to develop a method of determining fabric sheddability that was efficient, reproducible, and realistic under real-world conditions. There are several differences between the previous research and the current study. The use of a 3M post-it note as a less aggressive means for determining shedding is most notable. The use of a 2-pound weight, instead of hand pressure, to the applied post—it note makes this method less variable and therefore more reproducible. The current method yields a numeric value of shedding potential instead of categorizing shedding from “poor shedder” to “high shedder.” In addition, one type of recipient fabric was used in the transfer study to control the affect that recipient material has on shedding. A novel method was used for the transfer study in the current research. The forces experienced during contact between individuals during a primary transfer are difficult to predict. However, comparing results from a weighted cigar box transfer to those experienced in real life is inaccurate. Thus, this research intended to use a transfer method that reflected a common interaction, a hug, between two individuals. 14 METHODS AND MATERIALS To provide fiber examiners with an efficient, reproducible and reliable method for determining the sheddability of various fiber types, all materials used in this research were chosen to be inexpensive and commercially obtainable. The laboratory equipment used in this research is likely to be available in most forensic laboratories. Fabrics Chosen for this Research Thirty fabrics were bought at a JO—ANNTM Fabrics and Crafts® store for this study. One fabric, the acrylic, was donated and is manufactured by KIN KA FIBER CO., LTD., Taiwan. All thirty—one fabrics used were sole content fabrics. The exact percentages of fiber content for each fabric were not checked. However, in an attempt to verify that each fabric was composed of one type of fiber, fabrics were mostly bought from fabric bolts. Fabric bolts list the material description. SOme remnant fabrics were bought and also listed the fabric content. The following types of fabrics were obtainable at the authors’ local JO—ANNTM fabric store: cotton, polyester, rayon, wool, and nylon. The fabric samples were chosen to represent the variety of fabric types used in different garments. Twenty-four woven and seven knit fabrics were chosen. A full description of all thirty-one fabrics is in Appendix I. Warp and weft counts were determined for eighteen woven fabrics. A warp yarn is defined as the yarn which runs parallel to the selvage or longer dimension of a fabric.[5] The weft yarn runs perpendicular to the selvage or longer dimension of a fabric.[5] Twelve of those fabrics bought had the selvage end which is the long, finished 15 end of a fabric bolt. This provided a true fabric count. Appendix II outlines these eighteen fabrics. After purchase, fabrics were not washed, scraped, taped or vacuumed but used “as is”. The decision not to wash the thirty—one fabrics was based on the results of previous research by Colyer [l]. The research conducted by Colyer [1] tested the affect that age- related wear has on fiber shedding and subsequent transfer. By repeatedly washing several garments, Colyer found that garments shed less through time but do not do so in a consistent manner. Washing the thirty-one fabrics used in the current research is outside the scope of this project. The fabrics could have been washed and then tested using the post-it note method developed but this would have required washing each fabric piece thirty-one times using separate washing machines to eliminate contamination. In addition, each machine would have to be clean prior to subsequent washings. Materials Utilized for Determining Shedding Potential After purchase, the fabrics used in this study were cut into 12 x 12 inch swatches. These swatches were then divided into four, 6 x 6 inch squares using a ruler and a pencil. These large fabric swatches were packaged separately in plastic bags. Several packages of 100 sheet, 3 inch x 3 inch yellow 3M Post-it© Notes ([product number 654RP]) were purchased. One package of a 4 block, 50 sheet, variety colored pack of 2 7/8 inch x 2 7/8 inch 3M Post-it® Note was also purchased. The blue post-it note from this package was used on lighter colored fabrics for contrast. Figure 1 is an example of the materials utilized for determining the shedding potential of a fabric. 16 ’lgtlul'mi'ljll’lllllll'ljllllillll‘llli13!]1'13!”lll|ll||l'llljll|l|l|l‘l|:llllllllj'lIIIIIIIIu '- , ';‘-’j (Ch 312 .223 9330 fiflfifltfim wameuSmlcu 9: rl r ' n o n r r t ,2 ! IllllillllIflllill'lIIIll’llliIIlllIllllilillI:ll‘rllllIl”all!IIllllIllllIlMlIIHlIlllfillllltlllsllllllllllIllllIllIlilill’zllllilIll'lllllllll!m Figure 1: Example of Materials Needed for Determining Shedding Potential Two boxes of Xerox 8 1/2 x l 1 clear laser/copier transparencies were also purchased. A grid of five lines per inch was photocopied onto each of the transparencies. A two-pound weight is needed for the method developed in this study. For this study, the two—pound weight used was a heating block for PCR test tubes. Any object, which sufficiently covers the width of the post-it note, with a weight of exactly two pounds, will work. In addition to the above materials, a stopwatch or nearby clock is needed for this method. Method Utilized for Determining Shedding Potential With the aforementioned materials, the method developed for determining the shedding potential of fabrics is simple. As with any type of fiber examination, a clean working surface is crucial as well as a clean room. The ambient environmental effects of the laboratory were not controlled for while testing the post—it note method and therefore the resulting affect such factors as humidity had on shedding cannot be determined. The method used for determining the shedding potential of the thirty-one fabrics is as follows. A 12 x 12-inch piece of fabric, divided into 6 x 6 inch squares, was placed on a clean working surface finish side up. A post-it note was peeled from the top of the pack and placed adhesive side down onto an area within the 6 x 6 inch square. The two- pound weight was immediately placed on top of the post-it note, paying special attention that the weight covered the entire area where the adhesive was. The stopwatch was started and allowed to run for 5 seconds. After 5 seconds, the weight was removed and the post-it note was lifted off the fabric and placed, adhesive side down, directly on to the gridded transparency. The back of the post-it note was used to label the type of fabric being tested and the area, (i.e. upper left). The process was repeated in each of the four, 6 x 6 inch areas on the fabric. The procedure of post-it noting a fabric and transferring it to a transparency took roughly 13 seconds. Multiple post-it notes were placed on a transparency sheet by cutting the non-adhesive side of the post-it note off to allow for more space on the sheet. For pemranent storage, the edges of the post-it notes were taped down to the transparency using scotch tape and then the transparencies were stored in manila envelopes. The only laboratory instrument needed to obtain the numeric shedding value for a fabric using the above method is a compound microscope. In this study, fibers adhering to the adhesive side of the post-it note were counted using a Fischer Scientific compound microscope at a magnification of 45. Oblique, transmitted and reflected light were used. Both target and non-target fibers were counted separately. Target fibers were defined on 18 the basis of color. Varying light sources were utilized to aid in distinguishing between target and non—target fibers. Blue post-it notes were used to provide contrast with the light colored fabrics. The post-it note method was repeated four times for each fabric to obtain four measurements of shedding. An average of these four numeric counts was calculated to assign a Raw Mean Shedding Index (RMSI). Materials Utilized for Transfer Studies Fiber—transfer studies were performed with nineteen of the thirty-one fabrics tested using the previous method. The materials needed to perform this fiber-transfer study are easily obtainable and of minimal cost. The nineteen fabrics used as donor fabrics in the fiber transfer are marked with an asterisk in Appendix I. The recipient fabric chosen for this transfer study was white and black felt. Approximately ten yards of felt were purchased from a JO—ANNTM Fabrics and Crafts® store (nine were white and one black). After purchase the felt was cut into 12 x 12 inch swatches. The laboratory attire required for this transfer study included a tightly woven t-shirt and a laboratory coat. Participants in this study always wore light—colored t-shirts and disposable laboratory jackets buttoned to the collar during the procedures. Additional materials included boxes of large plastic storage bags, four large safety pins, and an ink pen. Method Utilized for Transfer Studies For this fiber-transfer study two participants were needed. The author recruited a fellow graduate student to assist her in this study. Both participants wore a light colored t-shirt and a laboratory coat buttoned to the collar. Since the laboratory being used for this research did not have a designated “clean room”, a room void of other fabrics or textiles was used to prevent transfer from items other than the fabrics being tested. Each participant safety pinned the fabric, either donor fabric or recipient felt, to the front chest area of their laboratory coat. A large plastic storage bag and an ink pen were always nearby. Handshake Transfer After securely fastening a piece of fabric to our laboratory coats, we proceeded to shake one another’s hand. To develop a reproducible handshake each time, we stepped towards each other and shook hands. Thus, the handshake method was: step forward, shake hands, release handshake and step back. The participant wearing the donor fabric would remove the recipient felt from the other participant and fold it twice over onto itself preventing the loss of any transferred fibers. The folded recipient felt was placed inside a plastic storage bag and labeled. This procedure was repeated with a new piece of recipient felt and a new donor fabric. Hug Transfer To develop a reproducible hug each time, the same two individuals performed all the hug transfers. Having the same two participants kept pressure relatively consistent. The method for the transfers via hug was as follows: step forward towards the other individual, embrace in a non-violent hug, release hug and step away. The participant wearing the donor fabric would remove the recipient felt from the other participant and 20 fold it up and then over onto itself preventing the loss of any transferred fibers. For each of the nineteen donor fabrics, the hug transfer method was repeated three times per donor fabric, using a new piece of recipient felt for each transfer. The folded recipient felts, were placed inside separate plastic storage bags and labeled Hug 1, Hug 2, or Hug 3. Materials and Method Used for Removing Transferred Fibers After the transfer study was completed, the recipient felts were examined for transferred fibers. The most efficient and common way of recovering transferred fibers is the tape method. For this research, the adhesive strip (5” x 7”) of a Lynn Peavey tape lifting kit was used. To conserve tape, the adhesive strips were cut in half horizontally. Each recipient felt was examined separately by removing the folded felt from the plastic storage bag and placing it on a clean working surface. The fabric was then carefully unfolded. The taping procedure consisted of removing the backing of the adhesive strip and placing the adhesive strip, adhesive side down, on the felt and rubbing the back surface of the tape with the forefinger. This process was repeated in a systematic manner until the entire piece of felt was taped. Adhesive strips were preserved by placing them adhesive side down on to a gridded transparency, which was then appropriately labeled. In this study, fibers adhering to the adhesive strip were counted under a Fischer Scientific compound microscope at 45X. Oblique, transmitted and reflected light were used. Both target and non-target fibers were counted separately. Target fibers were again defined on the basis of color. Fibers were counted and reported for the handshake transfers and for the hug transfers. Each donor fabric was subjected to three trials of the 21 hug transfer thus resulting in three tapings, from three pieces of recipient felt, for each hug. Labeling was important in this part of the study to maintain sample integrity. Repeating the hug transfer led to three “Hug” measurements. A mean of these measurements was calculated and for each of the nineteen fabrics tested a “Mean Hug Transfer” value was assigned. Statistical Methods Used If there were a perfect linear relationship between the two variables Raw Mean Shedding Index (RMSI) and the Transfer Mean, then for every fiber shed, one fiber would be transferred. This assumption will only be true if in the population the variable of interest (fabric sheddability) is normally distributed. Current and previous research has established that there are many variables involved in fabric shedding and fiber transfer. Due to these variables, fiber shedding is not normally distributed. Therefore, application of classic parametric statistics is not applicable to this research data. Another factor that limits the use of parametric tests to this research is the size of the sample data to be analyzed. Sampling distribution may be assumed normal, even if there is uncertainty whether the variable in the population is normal, as long as sample size is large (n 2 100). Only nineteen (n=19) out of the thirty-one fabrics were subjected to the hug transfer. To evaluate the data from this research a need for statistical procedures that allow for processing low quality data. from a small sample, on variables that are not normally distributed are needed. The application of nonparametric methods is therefore utilized for this research. When conducting the current research the calculated mean and standard deviation for the shedding index were not intended to be applied to a Gaussian distribution. The repeated measurements taken in this research were for internal consistency and for the ability to calculate a mean that was representative of the sample. To express a relationship between two variables one usually calculates the correlation coefficient. There is not a perfect correlation between Raw Mean Shedding Index (RMSI) and Hug transfer so the nonparametric equivalent to the standard correlation coefficient was needed. There are three commonly used types of nonparametric correlation coefficients. These include, Spearman R, Kendall Tau, and Gamma coefficients. Spearman R “assumes that the variables under consideration were measured on at least an ordinal scale, that is, that the individual observations can be ranked into two ordered series.” [9] Spearman R would be an appropriate test for this research data. The individual measurements of RMSI and Hug transfer can be ranked into two ordered series because intervals between numbers on an ordinal scale are not necessarily equal and the higher numbers of fibers shed or fibers transferred represent higher values on a ranked scale. Kendall Tau is similar to Spearman R but instead of computing from ranks, Kendall Tau “represents a probability that in the observed data the two variables are in the same order versus the probability that the two variables are in different orders.” [9] The hypothesis for this research questions the relationship between the variables RMSI and Hug transfer, not the probability that the two variables are different. Kendall Tau is not the appropriate method to apply. 23 Gamma is also a probability like Kendall Tau, but “it is computed as the difference between the probability that the rank ordering of two variables agree minus the probability that they disagree, divided by 1 minus the probability of ties.” [9] The gamma method is not applicable to this research because the data is too unpredictable to determine whether the factors affecting shedding and transfer are identical for the same fabric at the same time (probability of a tie). 24 RESULTS Each quadrant of a fabric was tested using the post-it note method and the corresponding target fibers were counted. The numeric values for these counts are designated as A, B, C, and D in Table 1. These values are in no specific order. A summary of the results for all thirty—one fabrics can be found in Appendix III. The numeric shedding value for the thirty-one fabrics used in this study was obtained by taking the average value of the four numeric counts. This number is reported as the mean shedding index. Standard deviation calculations were also performed and are reported. Woven Fabric A B C D Mean Shedding Index Std. Dev. [Poly. Hot Pink Fleece 64 17 6 8 24 27 [Non-Native Fibers 11 11 10 8 1O 1 Knit Fabric A B C D Mean Shedding Index Std. Dev. Cotton-maroon knit 343 264 328 444 345 75 Non-Native Fibers 19 6 31 3 15 13 Table 1: Example of Shedding Data for a Woven and Knit Fabric Intra-comparison of Raw Mean Shedding Index For this research, 8 polyester fabrics, 3 rayon fabrics, 10 cotton fabrics, 6 wool fabrics, and 3 nylon fabrics were tested. The raw mean shedding indices for each type of fabric were compared to investigate the differences in shedding between different fabrics of the same type. For example, the polyester fabrics tested in this research were both knit and woven fabrics. The fabrics varied in color and print effect. Some fabrics were intended to appear soft like a fleece material while others were tightly knitted for a smooth finished look. These differences contribute to the variation in shedding index among the same type of fabrics. 25 A Graphical representation of the intra—comparison of the polyester fabrics is shown in Figure 2. The graphs for the additional fabric types can be found in Appendix IV. F————’ .7017 * _ n," ’_f ,‘___._._v.___._— ’—“—u _.*.__._7 f i "a. 7 .i Polyester Fabrics l 300 — 250 ~ 200 ~- 150 a 100 a RMSI r-ltanlr—fij—r- 1 2 3 4 5 6 7 8 Fabrics Tested Figure 2: Raw Mean Shedding Index Comparison of Eight Polyester Fabrics Tested Fabric Structure and it’s Effect on Shedding After comparing the raw mean shedding index between fabric types it was relevant to investigate whether fabric structure had an affect on shedding. The woven fabrics chosen for this study included plain weaves, twills, satin, crepe, and a dobby fabric. There was an insufficient amount of knit fabrics obtained for this research. Due to this, no conclusions could be made on whether fabric structure has an effect on shedding. However, the graphical representation of this comparison warrants inclusion. (Figure 3) 26 500 ~ 1 450 J 400 i 350 ] 300 4 . . 250_ O IWOVENS l l l 9 'CKNEeG l l 200] ’ ‘ 1501 ‘ Q l 100 .- -, _ , , f . l .EL . r O 5 10 15 20 25 30 35 Fabfics Fibers Shed Figure 3: Plot of the Affect of Fabric Structure on Shedding A Fiber-Transfer Study Comparing the Raw Mean Shedding Index (RMSI) data of the fabrics’ demonstrated differences in the sheddability of the fabrics. The raw mean data itself divided fabrics into the following shedding categories: < 30 fibers shed, 30-60 fibers shed, and > 60 fibers shed. However, after calculating the medians for each fabric and plotting that data, natural breaks suggested five distinct categories. A light gray line at the mid—point between the adjacent values designates these “natural breaks” in Figure 4. This broke the data up into more categories and seems to fit the data better. Fabrics were categorized based on this division. 27 150 140 ~ 130 « 120 1 DataThreshold 110~ - 100 T 803 70 Fibers Shed 60 50~ 4o 30 _ 20 j H 10 - -'""_" Fabrics Figure 4: Data Yielding Five Shedding Categories Fabrics were categorized as the following: high shedders (X>80), high-medium shedders (80>X>46), medium shedders (45>X>26), medium-low shedders (25>X>l6), and low shedders (X>15) with X being the mean shedding index calculated after using the post-it note method. Mean shedding indices are reported in Appendix III. A representative sample of each fabric type and shedding category was chosen for the fiber transfer study. The categorization of fabrics used for the fiber transfer study is presented in Appendix V. A total of nineteen out of thirty-one tested fabrics, were used as donors for the fiber-transfer study. 28 The fiber transfer study itself involved two types of casual everyday contact, a handshake and a hug. Table 2 is an example of the number of fibers transferred during these simulated contacts. The hug transfer was repeated three times for each fabric. A summary of the results for the nineteen donor fabrics can be found in Appendix VI. Number of fibers transferred: HUG 1 HUG 2 HUG 3 HANDSHAKE Acrylic 170 165 83 NR Non-Native Fibers 3O 18 12 Poly.Black Suede-knit 757 576 456 3 Non-Native Fibers 113 101 94 5 Table 2: Number of Fibers Transferred during Hug(s) and Handshake The Hug Transfer No significant data was obtained from the handshake transfer. A mean was calculated from the data of the three hug transfers. The calculated means can be found in Appendix VII. The purpose of the transfer study was to answer whether there is a useful relationship between Raw Mean Shedding Index (RMSI) and transfer rate. The data for each was compared by plotting the ratio of RMSI to mean transfer for all nineteen-donor fabrics. Figure 5 exhibits this graphical data. The ratio comparison shows that fibers are being transferred from the fabrics more than shed over 50% of the time. This was expected since the pressure exerted during the hug transfer is higher than the pressure exerted by a two-pound weight. 29 50% 1.40 * O _ _-____._-_Q;_-z A E _ _,, __--_# _ 120 I‘ . Over-Estimates ’ o . . 9 0.80 e ~ -,u._zzz--__¢____,_____ — — O . Under-Estimates a s . “oeoeee— - O 0 o 0.40 -._-.g___9 ee —-—-——— O O. O Q 0 O 0.20 -2”- , mflz+ 2‘2 . O 0.00— o ’ . H e O. 20 Fabrics 4O 60 Under-Estimates by more than Figure 5: Ratio of Raw Mean Shedding Index to Mean Hug Transfer 30 Results of Spearman R Computation Nonparametric statistics are less statistically powerful than parametric methods, and if the goal is to detect small effects to the data then caution should be taken when choosing a test statistic. For the research at hand and the sample data collected, a nonparametric test like Spearman R is sufficient to determine whether there is a positive correlation between the variables RMSI and Hug transfer. Figure 5 shows that there is not a perfect linear relationship between RMSI and Hug transfer. However, there is a relationship between the two variables. The formula for Spearman’s Rank Correlation Coefficient is: Rs: ssxy/V(ssxyssxy) SSxx = Z(x—§)2 . SSW: 2(y-§)2 , ssxy=z(x—i)(y—§) Using the MINITAB Statistical Software Version 13 @2000, a Spearman R value was calculated. For this data set of n=19, the correlation coefficient equals 0.753. The data spreadsheet generated from the MINITAB software is shown in Appendix VH1. Repeated Hug Contacts Hug contacts were repeated to also study whether the number of transferred fibers decreased after repeated number of contacts. The number of fibers did decrease after multiple contact passes (hugs) for all nineteen fabrics tested. This data is presented in Appendix VI. 31 CONCLUSIONS Due to the complex nature of fiber transfer and persistence, it should be emphasized that the determined shedding index for the fiber types tested in this research be viewed as preliminary. However, the method developed for determining the shedding index of fiber types could be useful to a trace evidence examiner. The low adhesive backing of the post-it note did serve as a less aggressive means for determining shedding. The post—it note is a common laboratory supply that an analyst could quickly use to test the sheddability of a suspected source garment. Based on the fiber type of the source garment, the analyst could then classify the source garment as a high shedder, low shedder, or somewhere in between, using previously determined sheddability indices like the one presented in this research. The method parameters used in this research are reproducible, thus a laboratory could conduct a similar experiment to determine the shedding indices of common garments presented in casework. The fabrics used in this research do not completely reflect the types of garments presented in casework. The author did not have access to laboratory evidence and was constricted by the fabric stores available in the area. Garment types will also vary among laboratories based on the climate region that laboratory is located in. Therefore, it is best if each laboratory repeat the method for determining sheddability in their respective laboratory environment with the types of garments typically seen as evidence for that laboratory. If multiple laboratories participated in such research, cross sharing of data would be useful and could lay a foundation for a uniform interpretation of fiber evidence in forensic casework. 32 To determine the raw mean shedding index of a garment an examiner would need to repeat the post-it note method on several areas of the target garment. The post-it notes could easily be placed on a microscope slide and then screened using transmitted plane- polarized light for target fibers rather than a stereomicroscope. In this research, color was the only property used to pick out and count target fibers from the post-it note. In addition to color, the optical properties of the target fibers should be used to determine an accurate count for the shedding index. The transfer study conducted in this research project was unique compared to previous transfer studies. The classical transfer experiment usually involves a weighted cigar box with a recipient material attached that is pulled, with a constant pressure over a constant distance, across a donor garment. Although pressure, time, and distance is all controlled during these transfers, little can be extrapolated from such artificial contacts. These fixed conditions make the invalid assumption that transfer will be made by equal pressure on all areas of a recipient garment. Such conditions do not fit real-life transfers that occur in forensic casework. Due to the disadvantages found in the classical method, the goal of the transfer study in this research was to simulate a real life, everyday contact. When deciding on a method for the transfer study the factors of pressure, time, and area were considered. A simple gesture such as a hug often occurs between individuals in day-to-day contact. In order to hug, two individuals must come into contact. With a hug contact there is a form of pressure based on each individual’s body weight, strength, and enthusiasm behind hugging the other individual. Although, these three things may not be the only factors involved in such contact they are good variables to consider as a guideline for this transfer method. The contact area in a hug gesture is typically the front chest area of each individual. With all three factors considered, the hug transfer method used in this research project was developed. The hug transfer method requires fewer materials than the classic weighted cigar box. By using the same two individuals as participants in the hug transfer; the pressure of each contact could be relatively controlled. A rhythm to the gesture develops between the two participants that provides method reproducibility. The use of white felt as a recipient gamrent in this project reduced the number of variables involved in the transfer. Even though you seldom find in forensic casework a transfer between a donor textile and a white felt, the purpose of using white felt was to eliminate the affect the recipient has on shedding. The goal of this research was to first develop a reliable method for determining the shedding potential of a garment, and then to test whether this method was useful when evaluating fiber transfer. Therefore, the focus was on the donor material and the amount of fibers that material shed and transferred. The affect of the recipient material needed to be negligible. This research project generated two sets of data. The first set of data was the Raw Mean Shedding Index (RMSI) data that was based on the post-it note method developed. The second set was the mean Hug transfer that was calculated based on the multiple hug contacts performed with each fabric tested. As previously discussed, the RMSI data is a promising tool for a trace evidence examiner to use when evaluating fiber evidence. The data from the transfer is not meant to be used as a tool in forensic casework. The examiner cannot determine the variables involved in a transfer. Even with controlled transfer studies like this one and others in the past, the variables involved are too unpredictable. The transfer study was conducted as a means for evaluating the raw mean shedding index data. When the RMSI data and the mean Hug transfer data were compared by plotting the ratio of RMSI to mean transfer, the ratio comparison shows that fibers are being transferred from the fabrics more than shed over 50% of the time. This was expected since the pressure exerted during the hug transfer is higher than the pressure exerted by a two—pound weight. However, because such a large percentage of the nineteen fabrics tested were transferring fibers a significantly larger amount than shedding fibers, pressure alone is not the only variable involved. Only four of the nineteen fabrics tested actually shed more fibers on to the post-it note than transferred fibers on to the recipient felt. These four fabrics included the hot pink polyester knit, the wool twill, denim #1, and the meshed nylon. The RMSI values for the nylon meshed fabric and the wool twill fabric were not significantly greater than the mean Hug transfer. However with the denim fabric, the difference between the RMSI and the mean Hug transfer is sixty-eight fibers. The difference between the RMSI value and the mean hug value for the hot pink polyester knit is ten fibers. This value is not as large and it is difficult to conclude whether either is a significant finding. There could be many explanations as to why these four fabrics had a higher Raw Mean Shedding Index. The non-native fibers found on the fabric may have prevented fiber transfer. The pressure, duration and sequence of the hug method may have been altered. Fibers may have been misidentified and thus counted, on the post-it notes. The persistence of fibers transferred on to the white felt after the hug should also be considered. There is a possibility that fibers could have fallen off the white felt from the 35 time the hug contact took place to the time the felt was folded and packaged away. Any of these explanations might be true but were not tested for in this research. Repeating this research using similar fiber types but using optical properties as an additional guide to sorting target fibers could lead to answers regarding persistence. The most significant outlier in the data was the wool peach crepe fabric. Based on the low Raw Mean Shedding Index value (7), this fabric was categorized as a low shedder. In the transfer study, this fabric behaved like a high to hi gh-medium shedder having a mean transfer value of 182. Since the shedding potential for only one out of the nineteen fabrics tested was grossly incorrect, the success rate of using raw mean shedding indices to categorize the shedding potential of a textile has the potential to be 95% accurate. Additional differences in the raw data from the post-it note method and the hug transfer occurred between the cotton knit fabrics and the denim fabrics. Three out of the five cotton fabrics chosen for the transfer study were knit type fabrics. The only visible difference between the three was color. One knit was not dyed while the other two were maroon and teal in color. The finishing effect on all three fabrics was similar. The maroon and white cotton knits were categorized as high shedders while the teal cotton knit was categorized as a high-medium shedder. Although the white and maroon cotton fabrics were categorized as high shedders, the RMSI and transfer values for each were quite different. The shedding potential of the teal cotton knit was much lower than either of the white or maroon cotton knits. A difference in fiber diameter between these three fabrics may account for the differences in shedding potential. 36 Differences in shedding potential were also evident between the cotton denim fabrics chosen for the transfer study. The fabric structure for all three denim’s is right- handed twill. However the fabric construction and weight are different. Denim #1, the denim with the highest shedding potential, is brushed lighter weight denim. This denim was probably washed either chemically or mechanically before it was shipped to the fabric store. The washing loosens the fibers from the weave that in turn causes the fibers to pill, giving a brushed look to the denim. Loose fibers are easily she'd from a garment, which could explain why Denim #1 had the highest shedding potential. Denim #2, the denim with the lowest shedding potential, was stretch denim. Due to the stretch factor of this denim it is likely that this fabric my have a low percentage content of spandex and is not 100% cotton. The spandex content could have played a role in the shedding of the blue cotton fibers. Denim #3, the denim with a shedding potential between that of the other two denims, is rough heavy weight denim. This denim does not appear to stretch and has not been washed. How many other factors such as washing and fabric weight, affects shedding cannot be determined in this research but based on these results these variables should be considered when evaluating the shedding potential of a textile. Although a sound method was developed for determining the shedding potential of a fabric, the results of the rank correlation Spearman‘s r statistic were valuable to this research. This nonparametric statistic tested for correlation between the raw mean shedding index data and the transfer data. Without this test, an evaluation as to whether the post-it note method for determining shedding could be used to correlate with transfer evidence could not be made. The first step in finding Spearman’s rank correlation coefficient (r,) is to rank the values of each of the variables, RMSI and Mean transfer, separately. The Minitab software ranked the nineteen data points. The correlation coefficient is then computed in exactly the same way as the simple correlation coefficient. The only difference is that the values of x and y are ranks of the raw data rather than the raw data themselves. The accepted hypothesis (Ha) for this study is that the ranked pairs are positively correlated. The null hypothesis (H) would be no correlation between the ranked pairs. The rejection region is r8 2 r0. For this data set of n=l9, the Spearman’s rank correlation coefficient equals 0.753. Performing a one-tailed test with alpha equaling 0.005, r0 is equal to 0.608. Therefore, the null hypothesis is rejected. A positive correlated relationship exists between the raw mean shedding index and the mean transfer of a fabric. This ultimately means that a fiber examiner could use the Raw Mean Shedding Index for a fabric type to interpret the evidential value of fiber evidence in a forensic case. The Significance of a Sheddability Index in the Laboratory and the Courtroom The examination and interpretation of fibers in forensic science examinations is dependent upon a variety of factors. There have been a number of articles published on the evaluation of fiber evidence and the factors that influence the significance of such evidence. [18] Whether a factor has been determined to strengthen or weaken the fiber evidence the possible conclusions (excluding inconclusive) that can arise from a fiber comparison are still either: 38 a. The unknown fibers are similar to the known in all respects b. The unknown fibers are not similar to the known In the courtroom these conclusions are inadequate to the fact finders and should also be inadequate to the scientist. Fiber examiners are frequently being asked complex questions regarding the circumstances of the case, the type of transfer, degree of contact and the pressure of contact. Using Raw Mean Shedding Index data would assist an examiner in answering these questions. The assumption being that enough scientific testing is conducted between multiple laboratories, in various locations, where a solid database is created of the shedding potentials for common casework evidence. Take for example a case of fiber transfer, either one-way or two-way, where a victim was assaulted and killed. A suspect is apprehended and fibers are collected from the suspect’s clothing and environment. The shedding potential of all the clothing involved is determined. The following two by two matrix illustrates the possible scenarios a forensic trace examiner is faced with when evaluating the transfer evidence. High number of fibers transferred Low number of fibers transferred EXPECTED WHY SO FEW? lHigh Shedder Low Shedder WHY SO MANY? EXPECTED 39 In the first scenario, by using the post-it note method, the examiner determines that the source garment is a high shedder. There is also a large amount of fibers found on the victim. Since the source garment was determined to be a high shedder, finding a large amount of foreign fibers on the victim is expected. After analyzing the fibers the examiner concludes that the suspect fibers are similar to the source garment. In addition to these analytical results, the examiner can also assess the value of this fiber evidence by using the shedding index data. Based on the shedding index data, the examiner could accurately conclude that the presence of so many foreign fibers on the victim is considered to be indicative of recent contact and little movement of the body after contact. Another expected scenario is when the examiner determines that the source garment is a low shedder and finds only a small amount of fiber transfer evidence on the victim. After analysis, the suspect garment cannot be excluded as a source of the fibers found on the victim. The transfer evidence in this case could be more significant since finding fibers on the victim when the suspect was wearing a garment that was a low shedder is rare compared to if the source of the transfer evidence had been a high shedder In the above two scenarios, shedding index data supports the analytical data as well as provides further explanation as to the circumstances of the case, the type of transfer, degree of contact and the pressure of contact. Shedding index data can also help interpret the unexpected scenarios a forensic trace examiner is faced with when evaluating evidence. There may be a situation where the source garment has been determined to be a high shedder based on the post-it note method, but the number of 40 fibers recovered off the victim is low. This scenario does not mean that the post-it note method has failed to provide valuable information regarding the evidence. There can be several reasons why there is a lack of fiber transfer from a high shedding source. The most important thing to consider when evaluating this scenario would be the persistence of fibers after they have been transferred. Pounds and Smalldon [13-15] conducted the earliest studies on persistence. They found that the most significant factor in fiber persistence was the time of wear of the recipients after transfer of fibers. This could mean that a large number of fibers were shed and transferred from the high shedding garment but the initial rate of fiber loss was high due to an extended period from initial contact to victim discovery. Kidd and Robertson [8] also conducted a study on the persistence of fibers. They found that fiber persistence was lowered by several factors including: the length of wear, low pressure during initial transfer, and fiber size. These conclusions would support the possibility that the contact between suspect and victim was neither lengthy nor forceful. Lowrie and Jackson [8] conducted a study on the persistence of fibers on a wool jumper, an acrylic jumper, and a polyester jacket. They found that the combination of the nature of both donor and recipient had a significant effect on the results of their study. The authors found that smooth garments had the lowest levels of persistence for all types of transferred fibers. This study and others like it should be considered when evaluating the type of garment(s) involved in the transfer. In addition to the consideration of fiber persistence, another possibility of the high shedder—low transfer number scenario is that the victim’s body was moved or other evidentiary items were removed from the primary crime scene. This scenario may also 41 indicate that the fiber evidence was not from a primary transfer but in fact from a secondary or tertiary transfer. When faced with this high shedder-low transfer number scenario, an examiner must also consider that the unknown fibers could be a coincidental match to another similar source; while highly unlikely given clinical testing in the literature on incidental matches, it is still possible. The last scenario a fiber examiner could be faced with is where the source garment has been determined to be a low shedder based on the post-it note method, but the number of fibers recovered off the victim is high. Several reasons could account for this. The source garment could be torn or damaged causing more fibers to shed from the garment. Careful observation should thus be taken when examining the source garment. Evidence left at the crime scene of the cause of such damage would also be valuable and should not be overlooked. The low shedder-high transfer number scenario could also mean that contact between the victim and suspect may have been lengthy and forceful resulting in a higher number of fibers shed and transferred. Multiple contacts would also increase the amount of fibers transferred from a low shedder. Finding a high number of transferred fibers from a low shedding source could also indicate that a recent transfer occurred between the suspect and victim. In the low shedder-high number of fibers transferred scenario, it is more likely that local effects such as activity, garment damage, and repeated contacts would account for the transfer evidence more than a coincidental match. The high-fiber result from a low-shedder means an increased significance of the match. In reality, coincidental matches happen at a very low rate. 42 FUTURE RESEARCH Upon conclusion of this study, many additional areas of future research have been recognized as imperative for assessing the significance of the current findings. One very important addition to this research would be to conduct the research on a much larger scale. A larger data set would have to include using more fabrics in all the shedding categories, using fabrics that are typically found in forensic casework, using more knits, using more dyed and non dyed fabrics, and using printed fabrics. Conducting a larger scaled project would help identify all of the variables involved in shedding. Identifying all variables involved in shedding is crucial when evaluating the significance of a fiber transfer in forensic casework. Other research areas to be included in the larger scaled study would be to evaluate the affect a fibers cross—section and diameter have on shedding. This would entail gathering textiles of the same fiber type with different cross-sectional shapes and diameters and intra—comparing the shedding of those as well as comparing those textiles to the shedding potential of other fiber types. To the fabrics used in the current study an additional study could be conducted where the swatches were washed and dry cleaned and re-tested to determine the affect of washing on shedding index. The significance of a common occurrence such as garment washing could be evaluated and accounted for when determining the shedding index of a fabric. Another area of research interest would be to investigate the persistence of fibers on the recipient felt. The research question is essentially what would the average fiber loss be after x, y, and 2 time passed? These results would need to be related back to the 43 original shedding index for that donor fabric and considered when evaluating transfer evidence. With all of the above future research suggestions being considered for single fiber type (100% content) fabrics, using blended fabrics as an additional research variable has to be considered. Conducting this research study using blended fabrics may or may not help in determining the shedding potential of a blended fabric. This observation is based on the results of Parybyk and Lokans [12] study. However, blended fabrics do warrant consideration in determining shedding potential because they are prevalent in the garment industry. 44 Fiber Type *Acrylic *Polyester Polyester *Polyester Polyester * Polyester Polyester Polyester *Polyester Rayon *Rayon Rayon W001 W001 *Wool *W001 W001 *W001 *Cotton Cotton Cotton *Cotton *Cotton *Cotton *Cotton *Cotton *Cotton *Cotton *Nylon Nylon *Nylon Appendix I Fabric Description Fabric Structure Brown and came] sweater material Woven Black, brushed, suede-like appearance Knit Hot pink fleece material Woven Blue, silk-like feel as used for pant lining White, fleece material Plain Woven Hot pink shirt material Knit Pink w/orange, fuchsia & purple bubble pattern Crepe Woven Red w/white polka dot pattern Woven Navy blue shirt/suit material Knit Purple, black & pink animal print Plain Woven Red w/white daisy pattern Plain Woven Pink shirt material Plain Woven Tan Even—sided Twill Black Plain Woven Tan Twill Red, black & green plaid Dobby Fabric Navy blue/black shirt material , Warp-faced Twill Peach Crepe White fabric glittery pink snowflake pattern Plain Woven Bowling shirt-bowling scene pattern Plain Woven Sage green w/yellow box print pattern Plain Woven Teal Warp Knit Maroon Warp Knit White—Undyed Warp Knit Blue & white flannel Plain Woven Denim #1 Right-handed Twill Denim #2 Right-handed Twill Denim #3 Right-handed Twill Light blue mesh material Warp Knit Greenish-gray rain jacket material Plain Woven Creme smooth material Plain Woven *Denotes Donor Fabrics Used in Fiber-Transfer Study 45 Appendix 11 Fabric Description Acrylic-brown and camel sweater Rayon—red w/white daisy pattern Rayon-pink shirt material Rayon— purple, black & pink animal print Polyester-red w/white polka dot pattern Polyester-hot pink fleece material Polyester-white, fleece material Nylon-creme material Nylon—greenish-gray rain jacket material Wool- navy blue/black shirt material Wool-tan Wool—black Cotton-blue & white flannel Cotton-denim #1 Cotton-denim #2 Cotton-denim #3 Cotton-bowling shirt Cotton-sage green w/yellow box print pattern Cotton-white fabric w/effect face glittery pink snowflake pattern 46 Fabric Count 48 x 24 72 x 72 40 x 40 72 warps x 68 fillings 84 warps x 84 fillings 88 warps x 44 fillings 68 warps x 68 fillings 48 x 44 72 x 72 24 warps x 44 fillings 76 warps x 24 fillings 48 warps x 24 fillings 44 x 44 44 warps x 44 fillings 48 warps x 48 fillings 44 warps x 44 fillings 72 x 40 60 warps x 24 fillings 68 warps x 28 fillings Summary of Raw Mean Shedding Index Calculations Appendix III MEAN FABRIC *A *B *C *D STD. DEV SHEDDING INDEX Acrylic 80 44 70 61 64 15 Non-Native Fibers 0 0 0 0 0 0 fl’olyBlack Suede-knit 288 228 351 230 269 58 mortals/e Fibers 21 18 25 12 19 5 [Poly. Hot Pink Fleece 64 17 6 8 24 27 [Non-Native Fibers 11 11 10 8 1o 1 [Poly. Blue Silk-like feel 3 8 7 7 6 2 Mon—Native Fibers 1 0 1 1 1 1 [Poly. Wht. fleece material 9 7 6 8 8 1 [Non-Native Fibers 2 6 2 3 3 2 Ely. Hot Pink knit 52 64 58 54 57 5 [Non-Native Fibers 22 18 30 25 23 8 Ely. Pink w/orng bubble 19 15 23 16 18 4 [Non—Native Fibers 0 0 O O 0 0 “Poly. Red/white polka dot 19 16 30 18 21 6 Non-Native Fibers 4 14 1O 1 7 6 Poly. Navy blue knit 9 16 16 17 15 4 Non-Native Fibers 6 4 2 8 5 3 Rayon-Animal print 12 12 16 10 1 3 3 Non-Native Fibers O O 0 O 0 0 Rayon-Daisy Pattern 47 72 54 51 56 11 Non-Native Fibers 2 3 6 1 3 2 Rayon-pink 6 2 4 3 4 2 Non-Native Fibers 0 2 2 3 2 1 Tan Wool-evn-sided twill 29 33 24 41 32 7 Non-Native Fibers 26 25 14 15 20 6 47 A endix Ill (cont’d). Wool-Black 29 42 33 29 33 6 Non-Native Fibers 5 3 0 3 3 2 Wool Tan Twill 29 22 16 22 22 5 Non-Native Fibers 1 5 5 1 3 2 001 Native Fibers 001 shirt Native Fibers Wool-peach crepe 1 13 4 8 7 5 Non-Native Fibers 3 4 12 6 6 4 Cotton (pink snowflake) 22 35 14 23 24 9 Non-Native Fibers 12 9 14 15 13 3 Cotton Bowling shirt 25 4O 36 38 35 7 Non-Native Fibers 0 0 0 O 0 0 Cotton sage 9m wlyellow 23 27 28 30 27 3 Non-Native Fibers 2 4 4 5 4 1 Cotton-teal knit 66 62 64 27 55 19 Non-Native Fibers 28 69 37 21 39 21 Cotton-maroon knit 343 264 328 444 345 75 Non-Native Fibers 19 6 31 3 15 13 Cotton-white knit 110 112 131 109] 116 10 Non-Native Fibers 11 9 12 151 12 3 Cotton-flannel 26 30 42 36 34 7 Non-Native Fibers 1O 4 6 7 7 3 Denim #1 452 428 402 491 443 38 Non-Native Fibers 5 4 6 4 5 1 Denim #2 21 17 15 23 19 4 Non-Native Fibers 21 17 15 23 19 4 Denim #3 115 114 57 80 92 28 Non-Native Fibers 7 7 13 10 9 3 [Nylon-mesh 30 21 25 2O 24 5 [Non-Native Fibers 8 8 4 10 7 3 [Nylon-greenlgrey 12 11 16 13 13 2 [Non-Native Fibers 7 4 3 7 5 2 [Nylon-creme 7 18F 71 12] 11 4 RMSI Comparison of Raw Mean Shedding Index between the same fabric types Appendix IV Nylon Fabrics 1 2 Fabrics Tested 49 7! Appendix IV (cont’d). Cotton Fabrics 500 4+ .+ e- -221- 400 a 300 ' 200 l 100 - RMSI 1 2 3 4 5 6 7 8 9 10 Fabrics Tested Wool Fabrics RMSI 1 2 3 4 5 6 Fabrics Tested 50 A_._4 Appendix IV (cont’d). Rayon Fabrics 60 50 - 40 ~ 30 -. 20 a 10 - RMSI 1 2 Fabrics Tested 51 Appendix V Categorization of Nineteen Fabrics Tested Medium-Low Low High Shedders High-Medium Shedders Medium Shedders Shedders Shedders *X>80 80>*X>46 45>*X>26 25>*X>16 *X>15 mfimfl’l/W 710201!- DE/Wl/I/ WED/MM V/(IV/T WOOL-PLAID W CREME mmm RSV/”rt? MVWMSYPAWHV WWW/101%? M1 UNI/55W 677505 WNW/0W7: P01}! MW mm cvrrmmwr-mz DEN/III! . Bl (IE/UV” 7001K fill/E WWW/71W”? ACHfl/c m-mnmz SILK-(M PU! K 516W SUfDEAW/f *X: Denotes Raw Median Shedding Index Note: For clarification of specific fabrics noted above see Appendix II 52 Appendix VI Results of Hug and Handshake Transfers Number of fibers transferrred: HUG 1 HUG 2 HUG 3 HANDSHAKE Acrylic 170 165 83 NR Non-Native Fibers 30 18 12 Poly.Black Suede-knit 757 576 456 3 Non-Native Fibers 113 101 94 5 Poly. Blue Silk-like feel 19 17 16 NR Non-Native Fibers 87 95 85 Poly. Hot Pink knit 51 47 43 NR Non-Native Fibers 50 57 53 Poly. Navy blue knit 51 42 37 NR Non-Native Fibers 60 40 61 Rayon-Daisy Pattern 82 72 70 3 Non-Native Fibers 37 69 65 17 Wool-Plaid 1 00 52 40 NR Non-Native Fibers 54 30 19 Wool-peach crepe 297 140 1 10 NR Non-Native Fibers N/A N/A N/A Cotton (pink snowflake) 44 26 18 NR Non-Native Fibers 115 80 55 Cotton-teal knit 66 65 58 1 Non-Native Fibers 75 60 40 17 Cotton-maroon knit 542 535 437 2 Non-Native Fibers 54 106 70 15 Cotton-white knit 453 386 191 NR Non-Native Fibers N/A N/A N/A Cotton-flannel 135 82 78 N R Non-Native Fibers 62 28 31 Denim #1 451 372 302 4 Non-Native Fibers 40 37 25 6 Denim #2 81 51 48 NR Non-Native Fibers 91 55 69 Denim #3 144 89 76 NR Non-Native Fibers 28 14 20 Appendix VI (cont’d). Wool Tan Twill 22 21 8 2 Non-Native Fibers 65 46 75 13 Nylon-mesh 25 20 1 7 0 Non-Native Fibers 95 115 150 12 Nylon-creme 16 15 12 NR Non-Native Fibers 50 44 47 NR-no result obtained 54 Appendix VII Calculation of Mean Transfer DONOR FABRIC MEAN TRANSFER Acrylic 139 Poly. Black Suede-knit 596 Poly. Blue Silk-like feel 17 Poly. Hot Pink knit 47 Poly. Navy Blue knit 43 Rayon-Daisy Pattern 75 Wool-Plaid 64 Wool Tan Twill l7 Wool-Peach Crepe 182 Cotton (pink snowflake) 29 Cotton-Teal knit 63 Cotton—Maroon knit 505 Cotton-White knit 343 Cotton-Flannel 98 Denim #l 375 Denim #2 60 Denim #3 103 Nylon-mesh 21 Nylon-créme 14 55 Appendix VIII Results of MIN ITAB Statistical Program Pearson correlation of Hug and RMSI = 0.753 Fabric Hug RMSI Hug RMSI Rank Order Rank Order Acrylic 139 64 14.0 14.0 Poly. Black Suede 596 269 19.0 17.0 Poly. Silk-like feel 17 6 2.5 1.0 Poly.Hot Pink knit 47 57 7.0 13.0 Poly. Navy Blue knit 43 15 6.0 4.0 Rayon—Daisy Pattern 75 56 l 1.0 12.0 a Wool-Plaid 64 35 10.0 10.0 Wool Tan Twill 17 22 2.5 6.0 Wool-Peach Crepe 182 7 15.0 2.0 Cotton (pink snowflake) 29 24 5.0 7.5 Cotton-Teal knit 63 55 9.0 l 1.0 Cotton-Maroon knit 505 345 18.0 18.0 Cotton—White knit 343 l 16 16.0 16.0 Cotton-Flannel 98 34 12.0 9.0 Denim #1 375 443 17.0 19.0 Denim #2 60 19 8.0 5.0 Denim #3 103 92 13.0 15.0 Nylon-mesh 21 24 4.0 7.5 Nylon-creme 14 l l 1.0 3.0 56 10. ll. 12. REFERENCES . Colyer, A. M. Changes in fiber shedding characteristics as a garment ages. M.A. Thesis, Michigan State University. 1997. Coxen, A., Grieve, M. & Dunlop, J. A method of assessing the fibre shedding potential of fabrics. Journal of the Forensic Science Society. 1992; 32: 151-158. Grieve, M. Fibres and their examination in forensic science. Forensic Science Progress. 1990; 4:44-1 I9. . Greive, M., Dunlop, J. & Haddock, P. Transfer experiments with acrylic fibers. 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