1.91.31, #3:“: t ‘ . . . . ‘ , . . . . ‘ . ‘ , ‘ . . ‘ :1 , ‘ . . . . 9‘ Law. . . . a3" .. . . .A «x... .. \IJiQUW . a... #1253 k’ll‘l (5- In" .. . :... xr . an 3.64 a. . .Apx...‘ C... —. :va‘.1. . . 2 mark-aux §¥i§$v ., -18.) .. , V , ‘ , . . . .i. .. E .&.u4u.rufl., . . . . x , 9. ‘ J . ‘ ”a: ”.3”? “mug 1.44.4 I. c P. (9 LIBRARY ass-7 Michigan State University This is to certify that the dissertation entitled Assessing Physical Activity Behaviors in College Students presented by Joshua James Ode has been accepted towards fulfillment of the requirements for the PhD. degree in Kinesiology a Major Professor’s Signature 3/7, 07 Date MSU is an afiVnnetive-action, equal-opportunity employer --.-.-.—.-.--—.—._._._V-.-A._ 4 PLACE IN RETURN BOX to remove this checkout from your record. TO AVOID FINES return on or before date due. MAY BE RECALLED with earlier due date if requested. DATE DUE DATE DUE DATE DUE 6/07 p:lClRC/DateDue.indd-p.1 ASSESSING PHYSICAL ACTIVITY BEHAVIORS IN COLLEGE STUDENTS By Joshua James Ode A DISSERTATION Submitted to Michigan State University in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Department of Kinesiology 2007 ABSTRACT ASSESSING PHYSICAL ACTIVITY BEHAVIORS IN COLLEGE STUDENTS By Joshua James Ode Physical activity declines during the transition from adolescence to adulthood. In order to evaluate this transition, it is important to assess physical activity behaviors in the young adult population. This is possible by assessing college students, which represent a large proportion of the young adult population. Currently, the determinants of physical activity participation in college students are poorly understood and a more detailed understanding is needed to help prevent the decline in physical activity. PURPOSE: The purpose of this study was to assess the impact of gender, enrollment in a college activity- based class, and high school physical activity participation on physical activity behaviors in college students. A second purpose to this study was to assess the impact of enrollment in a healthy lifestyles course on change in physical activity during a semester. METHODS: A total of 911 college students enrolled in a healthy lifestyles class (n=455) and communications class (n=456) completed the baseline survey. A total of 765 students (healthy lifestyles = 355, communications = 365) completed the follow-up survey at the end of the semester. Study participants completed an intemet questionnaire assessing frequency, intensity, and duration of physical activity. Physical activity energy expenditure was specified as caloric expenditure indexed by body weight (kcal/kg/week) and quartiles of kcal/kg/week were used for analyses (Quartile 1: 0-125 kcal/kg/week, Quartile 2: 12.5-25.5 kcal/kg/week, Quartile 3: 25.5-45.9 kcal/kg/week, Quartile 4: >459 kcal/kg/week). A change score was used to assess the differences in physical activity at the beginning and end of the semester (Decrease: move to a lower quartile, Stay the Same: stay in the same quartile, Increase: increase to a higher quartile during the semester). Cross-tabulations were generated and chi square was used to determine any significant associations (p<.05) between physical activity participation and each exposure variable. Multinomial logistic regression was used to determine the magnitude of these associations. RESULTS: When compared to the lowest physical activity quartile, males had 7.2 higher odds than females of belonging to the highest physical activity quartile. There was no difference in physical activity between students enrolled in the healthy lifestyles class and communications class. College students who played 1 high school sport, 2 sports, 3 sports, or 4 or more sports were 2.12, 3.3, 3.1, and 5.2 times more likely to be in the highest physical activity quartile. Students who were classified as moderately active during high school leisure time physical activities were 2.5 times more likely to be in quartile 2, 3.7 times more likely to be in quartile 3, and 9.9 times more likely to be in quartile 4. Students enrolled in the healthy lifestyles class had 81% higher odds than communication students to increase physical activity. CONCLUSIONS: Despite the concern regarding physical inactivity on college campuses, little is known about physical activity in this population. Therefore, the results of this study provide valuable information about the determinants of physical activity in the college students and that enrollment in a healthy lifestyles class may have a positive impact on physical activity participation. Copyright by JOSHUA JAMES ODE 2007 ACKNOWLEDGEMENTS I would like to first thank my advisor, Jim Pivanik for his direction and mentorship throughout the past four years. Your dedication to your graduate students is unparalleled. I would also like to thank Dr. Mat Reeves for your time, effort, and for continually challenging me to think critically about research, Dr. Jim Anthony for the research opportunities you have provided, and Dr. Dan Gould for teaching me the importance of sport psychology. I would like to thank Jeremy Knous and Lanay Mudd for your contributions and support throughout this project and JoAnn Janes for always having the answer. Finally, I would like to thank my parents for their unconditional support, my wife Christina for your encouragement, understanding, and love, and my son Tyler for keeping me grounded. Acknowledgment of funding support: 0 This research was supported by a Student Awards Program grant fiom the Blue Cross and Blue Shield of Michigan Foundation. 0 This research was supported by a Plus One Active Research Grant on Wellness Using the Internet from the American College of Sports Medicine Foundation. TABLE OF CONTENTS LIST OF TABLES ........................................................................................................... viii LIST OF FIGURES ............................................................................................................ x CHAPTER 1 INTRODUCTION ......................................................................................... 1 CHAPTER 2 REVIEW OF LITERATURE ........................................................................ 9 2.1 Introduction ........................................................................................................... 9 2.2 Physical Activity ................................................................................................... 9 2.3 Physical Activity Assessment ............................................................................. 11 Introduction ...................................................................................................... l 1 Physical Activity Questionnaire ...................................................................... ll Reliability and Validity of Physical Activity Questionnaires .......................... 13 Conclusion ....................................................................................................... 19 2.4 Physical Activity in College Students ................................................................. 19 Introduction ...................................................................................................... 19 Review Studies of College Students’ Physical Activity Levels ...................... 20 Physical Activity Participation in College Students ........................................ 22 Physical Activity Levels among College Students Enrolled in a Health Related Class ........................................................................................ 31 2.5 Decline in Physical Activity from High School into College ............................. 36 Introduction ...................................................................................................... 36 Physical Activity during the Transition from High School to College ............ 37 Changes in Physical Activity between Adolescence and Young Adulthood ............................................................................................. 40 Conclusion ....................................................................................................... 45 2.6 Changes in Physical Activity During College .................................................... 45 CHAPTER 3 METHODS .................................................................................................. 48 3.1 Research Design .................................................................................................. 48 3.2 Study Population ................................................................................................. 49 3.3 Eligibility Criteria (inclusion/exclusion) ............................................................ 50 3.4 Study Participants ............................................................................................... 50 3 .5 Recruitment Procedures ...................................................................................... 50 3.6 Over-Arching Conceptual Model ....................................................................... 52 3.7 Assessment Protocol ........................................................................................... 54 3.8 Primary Outcome Variables ................................................................................ 56 3.9 Exposure Variables ............................................................................................. 58 3.10 Study Aim 1 ...................................................................................................... 62 3.11 Statistical Analysis for Study Aim 1 ................................................................. 62 3.12 Study Aim 2 ...................................................................................................... 66 3.13 Statistical Analysis for Study Aim 2 ................................................................. 66 vi 3.14 Supplementary Statistical Analysis ................................................................... 68 CHAPTER 4 RESULTS .................................................................................................... 71 4.1 Descriptive Data .................................................................................................. 71 4.2 Description of Study Aim 1 ................................................................................ 74 4.3 Assessment of Physical Activity Outcome Variable .......................................... 75 4.4 Study Aim 1 Descriptive Results ........................................................................ 76 4.5 Test of the Proportional Odds Assumption ......................................................... 79 4.6 Study Aim 1 Bivariate Ordinal Logistic Regression Results .............................. 81 4.7 Study Aim 1 Bivariate Multinomial Logistic Regression Results ...................... 84 4.8 Study Aim 1 Adjusted Multinomial Logistic Regression Results ...................... 88 4.9 Description of Study Aim 2 ................................................................................ 91 4.10 Time Varying Covariates .................................................................................. 92 4.11 Study Aim 2 Descriptive Results ...................................................................... 92 4.12 Unadjusted Bivariate Results ............................................................................ 95 4.13 Study Aim 2 Adjusted Multinomial Logistic Regression Results .................... 96 4.14 Results for Supplementary Statistical Analyses ............................................... 98 CHAPTER 5 DISCUSSION ............................................................................................ 103 5.1 Effect of Gender and Class on Physical Activity Participation ........................ 103 5.2 Effect of High School Sports and Other Leisure Time Physical Activities on Physical Activity in College .............................................................. 107 5.3 Effect of Enrollment in a Healthy Lifestyles Class on Change in Physical Activity ..................................................................................................... 110 5.4 Assessment of the Effect of Using Different Specifications of Physical Activity Energy Expenditure .................................................................... 113 5.5 Assessment of Supplemental Analysis Results on the Impact of Enrollment in a Healthy Lifestyles Class ................................................................ 117 5.6 Future Studies of Physical Activity in College Students .................................. 120 5.7 Conclusion ........................................................................................................ 122 APPENDIX A Consent Form .......................................................................................... 123 APPENDIX B Questionnaire ........................................................................................... 124 APPENDIX C Log Transformation Data for Kcal/kg/week ........................................... 129 APPENDIX D Results for Continuous Physical Activity Outcome Variable ................. 131 APPENDIX E Results for Categorical Physical Activity Outcome Variable ................. 133 APPENDIX F Study Aim 3 Research Design ................................................................. 136 REFERENCES ................................................................................................................ 147 vii LIST OF TABLES Table 1. Descriptive data comparison for the healthy lifestyles classes ............................ 72 Table 2. Descriptive data for the study population ............................................................ 74 Table 3. Descriptive data for quartiles of physical activity at the beginning of a semester .............................................................................................................................. 78 Table 4. Unadjusted ordinal logistic regression results for quartiles of physical activity at the beginning of a semester ............................................................................... 83 Table 5. Unadjusted multinomial logistic regression results ............................................. 87 Table 6. Adjusted multinomial logistic regression results for quartiles of physical activity at the beginning of a semester based on high school physical activity ................. 90 Table 7. Descriptive data for change in physical activity quartile ..................................... 94 Table 8. Unadjusted multinomial logistic regression results for change in quartile of physical activity during a semester .................................................................................... 96 Table 9. Adjusted multinomial logistic regression results for change in quartile of physical activity during a semester .................................................................................... 98 Table 10. Estimated relationship between physical activity energy expenditure and class membership during enrollment in a healthy lifestyles class (population-based approach) .............................................................................................. 99 Table 11. Estimated relationship between physical activity energy expenditure and class membership during enrollment in a healthy lifestyles class (subject-specific approach) ................................................................................................ 99 Table 12. Estimated relationship between physical activity energy expenditure at follow-up and class membership afier controlling for physical activity energy expenditure at baseline ..................................................................................................... 100 Table 13. Estimated relationship between physical activity energy expenditure at follow-up and class membership after controlling for physical activity energy expenditure at baseline and the nested structure of the healthy lifestyles class ............... 101 Table 14. Estimated relationship between physical activity energy expenditure and enrollment in the fall or spring healthy lifestyles classes ................................................ 102 viii Table 15. Mean, skewness, and kurtosis data for the continuous physical activity outcome variable .............................................................................................................. 129 Table 16. Mean, skewness, and kurtosis data for the log transformed continuous physical activity outcome variable ................................................................ 130 Table 17. Descriptive data for the log kcal/kg/wk .......................................................... 131 Table 18. Multiple regression analysis results for the log kcal/kg/wk ............................ 132 Table 19: Descriptive data for meeting the recommendations for physical activity ...... 133 Table 20. Unadjusted odds ratios for meeting the recommendations for physical activity ............................................................................................................... 134 Table 21. Adjusted odds ratios for meeting the recommendations for physical activity ............................................................................................................... 135 ix LIST OF FIGURES Figure 1. Timeline for data collection ................................................................................ 49 Figure 2. Over-arching conceptual model for Study Aim 1 .............................................. 53 Figure 3. Over-arching conceptual model for Study Aim 2 .............................................. 54 Figure 4. Graphical representation of physical activity quartile data ............................... 76 Figure 5. Graphical representation of the proportional odds assumption for the unadjusted analysis of race (African American vs. Caucasian) ......................................... 80 Figure 6. Graphical representation of the proportional odds assumption for the unadjusted analysis of class membership (Healthy Lifestyles vs. Communications) ....... 80 Figure 7. Change in odds ratios for gender after adjustment for exposure variables included in the adjusted multinomial logistic regression model ........................................ 91 Figure 8. Frequency distribution for the continuous physical activity outcome variable .............................................................................................................. 129 Figure 9. Frequency distribution for the log transformed continuous physical activity outcome variable ................................................................................... 130 Figure 10: Timeline for data collection for study participants entering the cohort in September ................................................................................................... 137 Figure 11: Timeline for data collection for study participants entering the cohort in January ........................................................................................................ 137 CHAPTER 1 INTRODUCTION The benefits of physical activity on overall health and chronic disease prevention are well known, yet many Americans are not physically active on a regular basis. Recent surveillance data have shown that only 45% of US adults 72 and 69% of high school students 7' met the American College of Sports Medicine’s and the Centers for Disease Control and Prevention’s minimum recommendations for physical activity. The recommendations are defined as either moderate activity (participating in activities that did not make the individual sweat or breathe hard for 330 minutes on 35 of the past 7 days) or vigorous activity (participating in activities that made the individual sweat and breathe hard for 320 minutes on 23 of the past 7 days). Furthermore, differences in physical activity prevalence between high school students and adults suggest a decline in physical activity as adolescents move into adulthood. This age related decline in physical activity is well documented, but poorly understood '5’ 60’ 68. Therefore, a more detailed understanding of the transition in physical activity behaviors from adolescence into adulthood is needed. College age students are a convenient population to study when evaluating the transition between adolescents and adults, as they may be sufficiently representative of the young adult population. According to the US Department of Education, 16.6 million students were enrolled in colleges or universities in 2004 and 29% of all young adults between ages 25-29 have completed a bachelors degree or higher '8. In addition, the most recent US Census Bureau report showed that nearly 53% of the adult population has been enrolled in some college based education 66. Due to the decline in physical activity that occurs during young adulthood and the large number of young adults enrolled in college, it is important to assess physical activity behaviors in college students. The American College Health Association, which published the Healthy Campus 2010 report, has recognized the importance of assessing physical activity on college campuses. The purpose of the Healthy Campus 2010 document is to establish national college health objectives which serve as a basis for developing plans to improve college student health. Healthy Campus 201 0 identified the Leading Health Indicators, which reflect major health concerns among college students and included physical inactivity as a priority health risk behavior 5 . To further illustrate the importance of studying physical activity behaviors in college students, results from a survey of recent college alumni found that nearly half of all college students reported being less active six years following graduation 65 . However, of the 44% of college students who reported exercising regularly while attending college, 85% engaged in similar or higher levels of physical activity following graduation 65. Therefore, there is a need for a detailed understanding of college students’ physical activity behaviors, as an active lifestyle during college may lead to an active lifestyle later in life. Most physical activity and health related data collected in college students are - I. .2 .4 . cross-sectlonal 6" .12 8 3 58 and have yielded inconsistent results. For example, prevalence estimates of college students who are physically active have ranged fi'om 33% to 59.7% in previous studies. This wide range may be due in part to the methods used to assess of physical activity, which often do not include questions needed to assess intensity, duration, and frequency of exercise. In addition, small convenience samples have been used which may not reflect the general college population. These criticisms were identified in a recent meta-analysis addressing college student physical activity. The authors concluded that comparing college students’ physical activity across studies is difficult, if not impossible, and there is a need for additional research that includes data on intensity, duration, and frequency of physical activity 36. Despite the many limitations in previous studies, the prevalence of meeting the recommendations for moderate and/or vigorous physical activity by college students is consistently lower than in high school students. Of four previous studies that assessed vigorous physical activity in college students, ” 44 26 48 the prevalence of college students meeting the vigorous physical activity guidelines ranged from 42-53%. These results were all substantially lower than the 65% prevalence rate of vigorous physical activity reported in high school students participating in the Youth Risk Behavior Surveillance System (YRBSS) which was developed to monitor the priority health risk behaviors among nearly 11,000 youth in the United States. Previous surveillance data suggests the prevalence of meeting the recommendations for moderate physical activity in college students was only 21%, 48 which is 5% lower than high school students participating in the YRBSS. Finally, a previous study reported that 58% of college students met the recommendations for either moderate or vigorous activity 57 which is lower than the 69% reported in the YRBSS. Interestingly, the prevalence rates for college students are consistently higher than those found for US. adults participating in the Behavior Risk Factor Surveillance System (BRF SS) which show a 25% prevalence for vigorous physical activity and a 46% prevalence for vigorous or moderate physical activity. These results illustrate the importance of assessing physical activity in college students as this time period may reflect an important period stage in the age-related decline in physical activity. A potential way to improve the physical activity levels in college students and potentially delay or reduce the age-related decline in physical activity is through enrollment in physical activity and health related classes. Pearman et al. 53 performed a cross-sectional study of 979 alumni fi'om two universities in which one required a physical activity class and one did not. Although the cross-sectional design of this study was a major limitation, alumni who attended the university where a physical activity course was required weighed significantly less, were more likely to know their blood pressure and cholesterol levels, and were more likely to engage in aerobic exercise than those who attended the university where a physical activity course was not required. These results suggest that enrollment in physical activity classes during college is beneficial 53. Despite the potential benefits, few colleges or universities currently require physical activity/healthy lifestyles classes for their students. In the 1960’s, nearly 90% of college institutions required physical education courses. This number has decreased to nearly 65% in the 1990’s 3 1. Often, a variety of elective physical activity and health related courses are offered to college students. However, due to a limited number of studies, data on the impact of these classes on physical activity levels are lacking 34. As a result, there is a need to examine the effect of enrollment in physical activity classes on college students’ physical activity behaviors. This information could be helpful in improving the content of and/or promote enrollment in health-related physical activity COlll'SCS. An important transition period in the age related decline in physical activity may occur as adolescents transition from high school to college. In order to illustrate specific details of the decline in physical activity during this period, Bray and Born asked first year college students about their current and past physical activity levels ”. Results indicated that only 33% of respondents were active in both high school and college, while another 33% were active during high school and became inactive during college 1'. There are minimal data to support the physical activity decline from high school to college as most studies are cross-sectional and were not designed to determine change in physical activity over time. The majority of longitudinal research focusing on this transition period assesses physical activity participation throughout adolescence and young adulthood. Telema and Yang 68 found that between the ages of 12 and 27, frequency of physical activity declined 57% and 28% in a large sample of males and females, respectively. In addition, total moderate or vigorous physical activity (defined by frequency and intensity) decreased 55% in males and 20% in females. Van Mechelen and colleagues 73 reported similar results in Dutch males and females in which there was a 31% (men) and 8% (women) decline in total time spent in habitual physical activity between the ages of 13 and 27. Furthermore, decreases in weekly energy expenditure of 42% in males and 17% in females occurred over the same time period. Although this research showed a decline in physical activity from adolescence to young adulthood, there is a lack of research that specifically assesses the transition from high school to university. This transition period is important as it is a unique experience for each individual that may include academic, social, cultural, emotional, as well as physical changes 24. Understanding the determinants for the age-related decline in physical activity is difficult as they may be specific to certain periods of life including the time spent at college. To the best of our knowledge, no longitudinal studies have assessed changes in physical activity and the corresponding determinants of these changes during the four years of enrollment in college and following graduation. Thus, we have limited understanding of the influence of college enrollment on physical activity levels, and there is a need to develop research protocols aimed at assessing this relationship. One purpose of this project is to develop a longitudinal research protocol which will effectively track physical activity behavior and its determinants over four years of college and following graduation. Study Summary: This investigation involves a detailed assessment of college student physical activity behaviors. Approximately 900 students were studied while they were enrolled in a healthy lifestyles course or communications course at Michigan State University. This dissertation focuses on a detailed assessment of college students’ physical activity levels. Currently, methods used to collect physical activity data in college students are inconsistent and often lack detail. Study strengths include: 1) a detailed assessment the frequency, intensity, duration, and type of physical activity, 2) assessment of the relationship between physical activity and a variety of demographic variables and other health related behaviors, 3) a retrospective assessment of high school sport participation and physical activity behaviors and 4) a longitudinal assessment of physical activity during enrollment in either a combined lecture/activity healthy lifestyles class or a communications course. An underlying objective of this dissertation is to determine if results change when using different physical activity outcome variables. Study Aim 1 includes two Specific sub-aims. Sub-aim 1 focuses on the impact of gender and membership in a healthy lifestyles class on physical activity behaviors at the beginning of a semester. Sub-aim 2 focuses on the impact of high school sport and physical activity participation on physical activity levels during college. The two sub- aims for Study Aim 1 are as follows: Study Aim 1 (sub-aim 1): Does gender or class membership influence physical activity energy expenditure in college students? Hypothesis: Male college students will be more physically active than female college students. Students enrolled in the healthy lifestyles class will be more physically active than students enrolled in the communications class. Study Aim 1 (sub-aim 2): Does high school sport and physical activity participation influence physical activity energy expenditure in college students? Hypothesis: College students who participated in high school sports will be more physically active than those who did not participate in high school sports. College students who were more physically active outside of high school sports will be more active than those who were not active outside of school sports. The second aim of this dissertation focuses on the impact of enrollment in a four- month combined lecture/activity healthy lifestyles class on change in physical activity levels following completion of the class. The aim includes a comparison of the change in physical activity between students enrolled in either the healthy lifestyles class or a communications class: Study Aim 2 is as follows: Study Aim 2: Does enrollment in a semester long healthy lifestyles class influence changes in physical activity energy expenditure? Hypothesis: At the end of the semester, college students enrolled in the healthy lifestyles class will have greater physical activity energy expenditure compared to students enrolled in the communications class. The third aim of this dissertation focuses on the development of methodology that will be used to collect longitudinal data on college students over a 4-year period. Data will not be collected as part of the study design development. Study Aim 3: To develop methodology needed to assess changes in physical activity and health related behaviors during enrolhnent as an undergraduate college student at a large Midwestern University. CHAPTER 2 REVIEW OF LITERATURE 2.1 Introduction The age related decline in physical activity across all ages is well documented, but the reasons for this decline are poorly understood 60. Specifically, there appears to be a decline in physical activity as individuals’ transition from adolescence into adulthood. However, there is a need for a detailed understanding of physical activity behaviors in the young adult population. It is possible that we can assess the transition between adolescents and adulthood by studying college students, as they are a convenient population to investigate. However, the literature on physical activity behaviors of college students is limited and there is a need for more detailed assessments within this population. Therefore, the focus of this literature review is to: 1) examine physical activity patterns in college students, 2) exarrrine changes in physical activity patterns as adolescents’ transition from high school to college, 3) examine the impact of enrollment in activity related courses on physical activity levels, and 4) examine the changes in physical activity participation that occur during college. 2.2 Physical Activity Physical activity is a broad and complex concept that can be defined as any bodily movement produced by skeletal muscles which result in a substantial increase total daily energy expenditure '6. Under the broad concept of physical activity, it is important to understand the differences between leisure time physical activity, exercise, sport, and occupational physical activity. Physical activity that increases total daily energy expenditure during an individual’s discretionary time is considered leisure time physical activity. When leisure time physical activity is performed repeatedly over an extended period of time with the intent to improve fitness, physical performance, or health, it is often called exercise. Sport is a form of physical activity that involves competition whereas, occupational activity refers to the energy expenditure required to meet the demands of a job. Leisure time physical activity, exercise, sport, and occupational activity are components of physical activity which is the only discretionary component of total daily energy expenditure. The relationship between physical activity and health is widely documented. Physical activity and exercise can prevent the occurrence of cardiovascular events, stroke, hypertension, diabetes, some cancers, obesity, depression, and can even delay mortality. As a result of the many benefits of increased physical activity, an important mission of the American College of Sports Medicine is to promote physical activity to the general public through the generation and promotion of public health recommendations for physical activity 4. In 1996, a Surgeon General’s Report titled Physical Activity and Health 70 provided what are currently well-established physical activity recommendations. This reports suggests that: 1) “Significant health benefits can be obtained by including a moderate amount of physical activity on most if not all days of the week.” and 2) “Additional health benefits can be gained through greater amounts of physical activity.” 10 2.3 Physical Activity Assessment Introduction The accurate measurement of physical activity is fundamental to studies assessing the relationship between physical activity and health. Physical activity energy expenditure can be measured using a variety of indirect methods including doubly labeled water 62 and accelerometry 59. Although these measures are often viewed as effective methods for measuring energy expenditure, they are not suitable for studies including large sample sizes. As a result, the most feasible method of collecting physical activity information in a large population is via self-reported questionnaires. Physical Activity Questionnaires A global assessment of physical activity is often the primary concern when assessing physical activity levels via questionnaire. However, physical activity questionnaires often vary widely in detail. For example, questionnaires may assess physical activity by categorizing individuals into dichotomous (active or inactive) or multiple categories (sedentary, low active, moderately active, or highly active). Most often, researchers use national recommendations for physical activity to create these categories. Physical activity can also be assessed as a continuous variable such as energy expenditure, which is often reported as the total amount of calories expended per day or week during exercise 64. Self-report questionnaires require an individual to recall physical activity levels over a specific time period ranging anywhere from the past day, week, month, or throughout a lifetime. Because the reliability of self-reporting information generally decreases over time, it is best to keep the reporting interval short 64. 11 Physical activity questionnaires focus on the type, intensity, frequency, and duration of physical activity performed and typically focus on activities that are most frequently performed 64. Intensity of exercise is most often reported in terms of metabolic equivalents (METS). One MET is the resting metabolic rate while sitting or lying quietly 2. Physical activity intensity is often expressed in multiples of METS and is the intensity of an activity relative to one MET. For example, an activity with an intensity of 3 METS is considered 3 times that of rest. The Compendium of Physical Activities 2 was developed to provide specific MET values for a variety of physical activities and is often used to determine the intensity of physical activity in survey research. Frequency is most often assessed by asking the number of times per week or times per month an individual participates in a given activity. This is a reasonable approach in making an overall assessment of physical activity 64. A second important aspect of frequency is whether the activity is performed during a single session or split into several smaller parts throughout the day. Thus, it may be important for surveys to distinguish between the two by documenting the total number of sessions per week rather than the number of days per week and individual participates in physical activity (’4. The duration of a single bout of physical activity is often expressed in terms of minutes per session or minutes per day. When duration is combined with the fi'equency of physical activity, total minutes of physical activity can be determined. When the total minutes of physical activity is combined with intensity of activity, energy expenditure can be calculated. Energy expenditure can be reported as the total amount of calories expended per week during exercise (kcal/week), the amount of calories expended per week during exercise relative to body weight (kcal/kg/week), or total MET minutes per week. Assessing physical 12 activity in terms of energy expenditure provides the most detailed method of assessing physical activity via questionnaires 64. Reliability and Validity of Physical Activity Questionnaires A major concern in physical activity survey research is the ability of a physical activity questionnaire to accurately assess an individual’s physical activity behaviors. As a result, previous research has focused on evaluating both the reliability and validity of physical activity questionnaires. The reliability of a questionnaire reflects its ability to yield the same result on multiple occasions. The validity of a physical activity questionnaire reflects the degree in which the survey measures the physical activity parameter it proposes. This section was limited to the review studies assessing the reliability and validity of commonly used physical activity questionnaires. A total of 11 studies assessing reliability and 6 studies assessing validity were reviewed. Reliability: The reliability of physical activity questionnaires is most often assessed using test-retest intraclass correlation coefficients which measure the degree to which two surveys administered on the same individual agree with each other. The reliability of two versions of the International Physical Activity Questionnaire (IPAQ) was assessed in adults between the ages of 18 and 65 across multiple countries. The short version of the IPAQ was designed to assess time spent walking, in vigorous activity, moderate activity, and sedentary activity over the previous seven days. The long version of the IPAQ was developed to collect more detailed information on the type of physical activity performed. Exercise intensity was assessed using MET values based on the Compendium of Physical Activities and total physical activity was reported in MET- l3 minutes per week. Data from 1880 adults across 12 countries were used to assess the test-retest reliability in both the long and Short forms of the IPAQ questionnaire. The one-week, test-retest reliability data were comparable between both forms of the IPAQ questionnaires showed a Pearson correlation coefficient (r) of nearly .80 in adults who completed the questionnaire '7 indicating good reliability. Lamb and Brodie 38 assessed the test-retest reliability of Liverpool Leisure-time Physical Activity Questionnaire (LLTPAQ) in 62 male and female British university staff and students. Total leisure time physical activity was assessed in terms of caloric expenditure by asking subjects to report activities performed over the past 14 days, the duration of each activity, and if sweating occurred during the activity. The test-retest reliability of the survey was assessed by inviting the subjects to return for a repeat survey 4 weeks later. The four-week test-retest reliability of total leisure time physical activity was r = .86 implying that the LLTPAQ is an excellent tool for recording physical activity. The Minnesota Leisure Time Questionnaire is another survey instrument also used to assess leisure time physical activity in adults. From a list of 63 different physical activities, subjects were asked to report activities performed over the prior 12 months. Energy expenditure was then calculated by asking subjects to report the number of months they participated in each activity, the average number of sessions per month, and the average time per session that was spent participating in that activity. The approximate calories per day of leisure time activity were then calculated. The test-retest reliability of this questionnaire was assessed in 147 adults over a five week period. Within this study population, the test-retest correlation coefficient for total leisure time physical activity was .88 indicating excellent reliability 22. l4 The Physical Activity Index from the College Alumnus Questionnaire was designed to estimate caloric expenditure over a one-week period while climbing stairs, walking, participating in sports, and participating in recreational activities. In order to assess the test-retest reliability of the questionnaire, the survey was repeated one month, eight months, and nine months following its administration at baseline. The test-retest reliability was high when measured at 1 month (r = .72), however, when assessed at 8 to 9 months, the test-retest reliability dropped substantially to r=.34 and r=.43, respectively. These results illustrate that the reliability of this instrument diminished as the recall period lengthened 3. In order to offer a more global perspective of reliability, a simultaneous evaluation of the reliability of multiple physical activity questionnaires was assessed in 78 subjects varying in age from 20-59 33. The test-retest correlation coefficients over a one-month period exceeded .92 for total physical activity in the Minnesota Leisure Time questionnaire, the Lipid Research Clinic questionnaire, and the Baecke Physical activity questionnaire. The one-month test-retest reliability for the Godin Leisure time physical activity questionnaire (.62), the health insurance plan of New York (.86), The CARDIA physical activity history questionnaire (.88), the College Alumnus questionnaire (.72), the Minnesota heart health program (.86), and the Stanford usual activity questionnaire (.77) showed moderate to high reliability. These results illustrate that most physical activity questionnaires are reliable over a short time period. Booth and colleagues 7 assessed the reliability of the World Health Organization health behavior in schoolchildren survey. The questionnaire was administered on two separate occasions, two weeks apart and the test-retest reliability was assessed by 15 measuring percentage of agreement rather than using the preferred method of correlation coefficients. Agreement was calculated as the number of students classified as active at both occasions plus the number of students classified as inactive at both occasions, divided by the total population. When frequency and duration of physical activity were assessed together, there was 67% agreement among 14 year old boys, 70% agreement among 14 year old girls, 85% agreement among 16 year old boys, and 70% agreement among 16 year old girls. The agreement shown between the two-week time period in this study is considered substantial 39. Validity: The validity of a questionnaire reflects the degree to which physical activity questionnaires measure what it is propose to measure. Compared to reliability, validity is assessed less often due to the absence of a widely accepted criterion measure of physical activity 64. Despite this, validity remains a critically important issue in the assessment of physical activity questionnaires. Most often, the validity of physical activity questionnaires is determined by comparing the questionnaire to another measure of physical activity such as doubly labeled water or accelerometry. The doubly-labeled water technique is particularly useful for measuring energy expenditure over relatively long periods of time (a few days or weeks). However, the validation of a physical activity questionnaire against double labeled water is costly and as a result most validation studies include only small sample sizes. The validity of three physical activity questionnaires was compared to physical activity levels measured by doubly labeled water in 19, 40-year-old males 55. The Baecke physical activity questionnaire assessed four indices of physical activity: work index, sport index, leisure time index, and the total activity index. The Five City Project questionnaire assessed l6 physical activity levels by asking the frequency a sweating during vigorous activity in combination with the number of hours of vigorous activity per week. The Tecumseh Community Health Study questionnaire assessed daily energy expenditure by assessing the number of hours spent in various physical activities per week. The Baecke Activity Questionnaire showed the highest correlation with doubly labeled water (r=.69). Total daily energy expenditure measured by the Tecumseh Community Health Study questionnaire also showed a high correlation (r=.64) with double labeled water. There was also a good correlation (r=.5 7) between the sweat index measured by the Five City Project questionnaire and double labeled water 55. Furthermore, when compared to doubly labeled water, the validation of the Physical Activity Scale for the Elderly showed similar correlation coefficients for both males (.79) and females (.68) 63. The results of these previous studies suggest that physical activity questionnaires show good validity when compared to doubly labeled water. Validation studies performed in larger samples often use an accelerometer because it is a less expensive method to assess physical activity. In fact, the International Physical Activity Questionnaire was assessed against accelerometry in 744 adults across 12 countries. Correlation coefficients were calculated to assess the validity and the results illustrated only a fair to moderate agreement between the two measures (r = .33) '7. Philippaerts and colleagues 54 also assessed the validity of two physical activity questionnaires, the Tecumseh Community Health Study Questionnaire and the Beacke Activity Questionnaire, using accelerometry as the criterion variable. Accelerometer data were collected over four consecutive days in 166, 40 year old men. Both the Tecumseh l7 Community Health Study Questionnaire and the Beacke Activity Questionnaire showed a moderate validity (r = .47 and r = .32, respectively) compared to accelerometer data. Physical activity diaries are also used as an additional method of validating physical activity questionnaires. The College Alumnus questionnaire was validated against a 48 hour physical activity diary. Seventy eight adults between the ages of 21 and 59 recorded all physical activity at least every four hours in a diary designed for the study. Following the 48 hour period, the subjects completed the College Alumnus Questionnaire. Correlation coefficients between the two measures were calculated and results showed that the physical activity index of the College Alumnus Questionnaire moderately agreed with the physical activity diary (r = .42) 3. Aadahl and Jorgensen ' assessed the validation of a physical activity questionnaire for measuring sport, work, and leisure time activities against a 4-day physical activity diary. Using the Compendium of physical activities, total MET time was calculated by assessing the number of hours and minutes spent on each MET activity on an average weekday. The validity of the physical activity questionnaire was assessed in 20 men and 19 women. Within the diary, participants were requested to report the type, duration, and intensity of all activities, 24 hours a day, for a four day period. The agreement between the activity questionnaire and the diary was then assessed and the results illustrated the correlation between physical activity questionnaire and the diary was high (r = .74) indicating that this self-report physical activity questionnaire was valid. 18 Conclusion The ability of a questionnaire to: 1) yield the same result on multiple occasions and 2) to measure what it is proposes is fundamental to the accurate assessment of physical activity. Thus, previous research has examined both the reliability and validity of a variety of physical activity survey instruments. Previous studies illustrate the test- retest correlation coefficients of well-known physical activity surveys range from .72-.92 over a one month period. These results suggest that questionnaires assessing physical activity are reliable over a short time period. The validity of physical activity questionnaires has been assessed against a variety of physical activity measures. As a result, correlation coefficients have ranged from .32 to .79 in studies using doubly labeled water, accelerometry, or physical activity diary as the comparison measure. Despite the wide range of correlation coefficients, there appears to be a moderate to high relationship when comparing questionnaires to other forms of physical activity measures. These results illustrate that questionnaires are a sufficiently accurate method for assessing physical activity in population based studies. 2.4 Physical Activity in College Students Introduction Recently, the American College Health established national college health objectives in a document titled “Healthy Campus 2010: Making It Happen” 5. Healthy Campus 2010 is a companion document to the healthy People 2010 and serves as a basis for developing plans to improve student health. Healthy Campus 2010 has developed over 200 health objectives with baselines and targets for the nation's colleges and 19 universities. Physical activity is one of the top ten leading health indicators within this document. The two specific physical activity objectives of Healthy Campus 2010 include: 1) increasing the proportion of college students who have received information on physical activity and fitness and 2) increasing the proportion of college students who engage in physical activity at least 3 days/wk at moderate intensity for at least 30 minutes, or vigorous physical activity for 20 minutes or more minutes. In order to determine whether Healthy Campus 2010 goals are met, detailed assessments of college students’ physical activity levels are needed. Review Studies of College Students ’ Physical Activity Levels Despite the Healthy Campus 2010 goal to improve physical activity levels among college students, the current literature to assess physical activity behaviors in college students is inadequate. This point has been illustrated in two systematic reviews conducted specifically to interpret the previous literature assessing physical activity in college students. To the best of our knowledge, these are the most thorough studies that have been performed to assess physical activity participation in college students, and the purpose of this section is to describe their results. Recently, a review article on physical activity behaviors in college students addressed three key issues: 1) previous research on students’ physical activity levels, 2) problems surrounding current research on this topic, and 3) suggestions for further research 36. This review illustrated that a glaring limitation among studies assessing college students’ physical activity levels is inconsistent measures used to assess physical activity. Currently, there is no consensus regarding the use of standardized questions to 20 assess physical activity in a college population. Although energy expenditure is calculated using intensity, total time, and frequency of activity, the studies assessed in the review used various combination of the three including: 1) frequency and intensity of specific activities, 2) total weekly minutes of physical activity regardless of intensity, and 3) only frequency of physical activity. The authors concluded that these inconsistencies in t physical activity measurement makes it nearly impossible to summarize current physical activity patterns in this population 36. In order to assess the prevalence of university students meeting the American College of Sports Medicine/Centers for Disease Control and Prevention (ACSM/CDC) guidelines for moderate activity (students who accumulated 30 minutes or more of moderate physical activity on most, preferably all, days of the week), a systematic review including 19 studies representing nearly 36,000 students from a total of 27 countries was performed 32. Results Showed that approximately 50% of university students within the United States, Canada, and China did not meet the recommendations for moderate physical activity. Within Europe and Australia, the prevalence for not meeting physical activity recommendations was 67% and 40%, respectively. However, the authors cautioned that lack of a standard method of physical activity assessment is a major limitation when assessing those data. Two general conclusions were drawn fiom the results of this study. First, insufficient physical activity is a serious health problem among university students. Second, there is a need for a standardized tool to assess physical activity within college students to better track their physical activity participation 32. 21 In conclusion, the two systematic reviews assessing physical activity participation in college students illustrate similar methodological limitations within the previous research. These limitations include the inconsistent measurement of frequency, intensity, and duration of physical activity participation. Although these studies conclude that physical inactivity is a problem within the college population, researchers should strive to assess physical activity participation in a more consistent manner. This will offer a more detailed and consistent perspective of physical activity behaviors within this population. Physical Activity Participation in College Students Healthy Campus 2010 included physical activity as a priority health risk behavior based on its relevance as a broad public health issue 5 . As a result, it is necessary to determine the proportion of the general college student population who participate in adequate amounts of physical activity and to determine what characterizes physical activity participation in this population. The search terms “physical activity”, “exercise”, “college”, “university”, and “student” were used in various combinations to conduct the literature search via the National Library of Medicine to identify all relevant articles. This was followed by a careful examination of literature cited pages to identify additional relevant studies that were overlooked. To our best knowledge, twelve studies have assessed physical activity participation in a general population of college students, all of which will be reviewed in this section. In addition, this section will be organized by the method of physical activity measurement. First, the six studies reviewed in this section assessed physical activity participation as meeting the ACSM/CDC national recommendations for physical activity. It should be noted that these recommendations 22 for moderate physical activity (30 minutes per day, five or more days per week) are different that the Healthy Campus 2010 goals for moderate physical activity (30 minutes per day, three or more days per week). To the best of our knowledge, no previous studies the criteria of Healthy Campus 2010 to assess moderate physical activity. Second, the Single study that estimated total caloric expenditure was reviewed separately. Finally, the remaining five studies that used various combinations of frequency, intensity, and duration of physical activity were grouped. Meeting the national recommendations for physical activity: The American College of Sports Medicine recommends that every American adult accumulate at least 30 minutes of moderate physical activity on most days of the week or 20 minutes of vigorous physical activity on 3 days per week 4. When assessing recommendations, moderate activity is most frequently defined as participating in an activity such as walking, slow biking, or gardening that did not make you sweat or breathe hard. Vigorous activity is most fiequently defined as participating in an activity such as jogging, running, rowing or other activities that made you sweat and breath hard. These recommendations are often used to assess college student physical activity participation. In order to monitor health risk behaviors among high school youth in the United States the Youth Risk Behavior Surveillance System (YRBSS) was developed. A component of the YRBSS is the Youth Risk Behavior Survey (YRBS) which assesses a variety of health related behaviors including physical activity participation in high school students. An additional component of the YRBSS is the National College Health Risk Behavior Survey (N CHRBS) which focused specifically on health risk behaviors within the college population. The NCHRBS was administered in 1995 and used a nationally 23 representative sample of undergraduate college students fi'om 136 universities across the United States. A random sample of students was drawn from a list of undergraduate students older than 18 years. To ensure adequate proportions of minority students, differential sampling rates were used. A total of 8810 students were selected from the 136 colleges and universities and data fiom 4609 undergraduates were available for analyses (60% response rate). Of the 4609 college students, 64% were between the ages of 18 and 24. The results showed that 42% of students between the ages of 18-24 participate in adequate levels of vigorous activity while 21% participate in adequate amounts of moderate physical activity 48. In addition, NCHRBS results indicated that male students (49%) were more likely to report vigorous exercise than female students (35%) and that black students (28%) were significantly more likely to report moderate activity levels than white students (18%). Physical activity participation during college was assessed as part of a cardiovascular needs assessment in 607 first year students enrolled in a Nova Scotia University 44. Results showed that 51% of the students did not participate in adequate amounts of vigorous physical activity. Of students who reported any physical activity, the most frequently reported mode of activity was walking (64%). In addition, there was a positive relationship between perceived knowledge of cardiovascular benefits of physical activity and frequency of physical activity participation, which illustrate the importance of increasing physical activity knowledge in college students 44. Gyurcsik and colleagues 26 assessed vigorous physical activity participation in 132 female freshmen enrolled in their first-year of college immediately following high school graduation. Participants were asked to indicate the number of times they engaged 24 in at least 20 minutes of vigorous physical activity during a typical week over the previous month. The average frequency of vigorous physical activity was only 2.8 weekly sessions and 47% failed to meet the national recommended level of vigorous physical activity 26. Vigorous physical activity participation during the first two months of college was assessed in 145 first year college students attending a liberal arts university in Canada ”. The students who reported participating in an average of three or more bouts of vigorous activity for 20 minutes or longer per week during the first two months of college were classified as active. Forty four percent of the college population was active during the first two months of college ”. It is possible that the setting in which one exercises has an effect on the meeting the recommendations for moderate intensity physical activity. In order to assess the impact of exercise setting, self-reported physical activity was assessed in 594 first and second year university students '3 . Students were asked to identify the frequency, duration, and intensity of physical activity when participating in four different exercise settings: 1) aerobics classes, 2) with others outside of a structured aerobic class, 3) alone in an exercise setting, and 4) completely alone. Six percent of the population reported no involvement in physical activity within the four listed settings. Of those students who reported involvement in a physical activity setting, the average frequency and duration of exercise was 4.5 days per week and 52 minutes, respectively. Within the total sample, 43% met the national recommendations for vigorous activity. Only a small percentage of students (10%) who met the recommendations were physically active in only one exercise setting, whereas 28% obtain their activity in at least two contexts and 62% 25 obtain their activity in three or more contexts. When compared to the perecentage (10%) of students who met the physical activity recommendation, a larger percentage (30%) of active students who did not meet these recommendations obtained their activity in only one settings (30%). In addition, only 33% of students who did not meet the recommendations exercised in three or more settings compared to 62% in those who did meet the recommendations. These results suggest that exercising in multiple settings are associated with improved adherence to the ACSM/CDC national physical activity recommendations 13. Pinto and colleagues 57 examined self reported physical activity data in 332 first- year college students enrolled in a private university. Students were divided into two groups. Students who reported either: 1) 20 minutes of vigorous physical activity (exercise that made you sweat and breath hard) three or more days per week, or 2) 30 minutes of moderate physical activity (exercise that did not make you sweat and breathe hard) five or more days per week were met the recommendations and were classified as “active”. Results indicated that 58% of the sample exercised at or above the recommended level and 42% did not 57. In summary, these study results suggest that a substantial amount of college students do not meet the current physical activity recommendations. Inadequate levels of physical activity ranged from 42% to 56% in these studies. In order to track improvements, researchers should continue to assess college students’ physical activity in terms of meeting the national recommendations for both vigorous and moderate activity. Physical activity caloric expenditure in college students: To provide a more detailed assessment of physical activity via questionnaire, caloric expenditure can be 26 calculated using data gathered from questions assessing the frequency, intensity, and duration of physical activity. To our knowledge, only one study reported physical activity levels in terms of caloric expenditure in a general population of college students. Leslie and associates 43 identified insufficient physical activity among 2,729 Australian students enrolled in two metropolitan and two rural universities. A self reported 2-week physical activity recall was used to assess the fi'equency and duration of walking for recreation, moderate physical activity, and vigorous physical activity. Metabolic equivalents were used to estimate energy expenditure over a 2-week period. Within the male student population, 8% were classified as sedentary (< 100 kcal/week), 24% were low-active (100-799 kcal/week), 42% were moderately active (>800 kcal/week, but not in vigorous category), and 26% were highly active (>1600 kcal/week including three sessions of at least 20 minutes of vigorous activity). Eleven percent of females were sedentary, 36% were low-active, 36% were moderately active, and 16% were highly active. This analysis was extended to examine associations of personal, social, and perceived environmental variables in relation to physical activity status. In comparison to less active students, sufficiently active college students were more aware of campus recreational facilities, had a high enjoyment of physical activity, high social support fi'om family and friends, high self-efficacy, were employed, and were members of a gym. Physical activity in college students using various methodologies: As referenced by two previous review studies 32’ 36, the biggest limitation in evaluating physical activity in college students is the inconsistent methodology used to assess physical activity. The purpose of this section is to review studies that used various combinations of fi'equency, 27 intensity, and duration of physical activity to assess physical activity participation in college students. A large cross-sectional survey assessing leisure time physical activity in over 19,000 college students across 23 countries was performed in 2004 to analyze differences in physical activity prevalence. Leisure time physical activity was assessed by asking the type and frequency of physical activity performed over the past two weeks. Subjects were divided into three groups: inactive (0 days of exercise per week), low-frequency activity (1-2 days of exercise per week), and recommended frequency activity (exercising three or more times per week). Overall, more women (3 8%) then men (27%) reported no leisure time physical activity. When comparing countries, inactivity levels were lowest in Western Europe and the United States for both men (24%) and women (21%). The highest rates of physical inactivity were seen in developing countries which included Colombia, South Afiica, and Venezuela (35% in men, 53% in women) 27. A major limitation to this study was that intensity and duration of physical activity was not used to describe physical activity levels. Sparling and Snow 65 assessed physical activity levels in recent college alumni. A survey was administered to 376 individuals who recently graduated from college. Physical activity during college was assessed retrospectively by asking the alumni to report their physical activity patterns during their final year of college. Nearly 43% of the sample considered themselves to be regular exercisers (3 or more days of physical activity per week) during college, 40% as irregular exercisers (1-2 days of physical activity per week), and the remaining 17% as non-exercisers (less than one day of exercise per week). The proportion of men who categorized themselves as regular 28 exercisers was 45% compared to 39% of women. Interestingly, 85% of study participants who were regular exercisers during college were either as active or more active following graduation and 81% of non-exercisers reported being the same or even less active following graduation. These results suggest few individuals changed there physical activity participation soon after graduation. Self-reported participation in physical activity was analyzed in 217 college students attending a private, liberal arts university in Rhode Island. Physical activity data were obtained fi'om undergraduate students completing a survey assessing satisfaction of university health care service. Both type and frequency of physical activity were assessed and individuals who exercised three or more times per week were considered active. The results illustrated that 54% of the population was currently active, whereas 28% exercised up to two times per week. The remaining 18% were considered inactive. There were no significant differences between gender and class across the three groups. Furthermore, students reported participating in running, weight lifting, and cycling most often 58. Dinger and Waigandt '9 collected physical activity data on over 2600 college students enrolled in a large Midwestern university. F orty-five percent of the sample reported participating in at least 3 days of vigorous activity during the previous week while 22% did not report participating in vigorous activity. F orty-six percent of the population participated in moderate physical activity three or more days per week. However 30% of the sample did not report participating in moderate physical activity the previous week. On average, males participated in an average of 2.8 days of vigorous physical activity per week, 2.4 days of moderate activity per week, 1.9 days of flexibility 29 exercises per week, and 1.8 days of muscular strengthening exercise per week. In comparison, females participated in an average of 2.2 days of vigorous physical activity per week, 2.9 days of moderate activity per week, 2.2 days of flexibility exercises per week, and 1.5 days of muscular strengthening exercise per week. Inappropriately high levels of physical activity in college students has also been examined. A study by Garman and colleagues 25 examined the occurrence of excessive exercising in a college population. A total of 268 students completed a self-report questionnaire designed to assess frequency, duration, and intensity of physical activity. Participants were considered to be “exercise dependant” if they: 1) exceeded 360 minutes of exercise per week and 2) pursued physical activity when it could compromise health or supersede social and/or academic responsibilities. Of the 22% of the sample who were considered exercise dependent, 53% were male. The average time spent exercising within the exercise dependent group exceeded 730 minutes per week. Within the population of college students who were considered general exercisers (students participating in less than 360 minutes of exercise), an average of 103 minutes of physical activity was performed per week. The average frequency of physical activity participation was just under three times per week in the general student population. In summary, a variety of different methods have been used to assess physical activity participation in college students. Although varying methodologies make direct comparison of results between previous studies difficult, the majority of findings indicate a large proportion of these students are not physically active. Therefore, future research should include detailed assessments of physical activity to better describe the current physical activity insufficiencies within the college population. 30 Physical Activity Levels among College Students Enrolled in a Health Related Class Much of the physical activity research in college students is collected when they are enrolled in health related courses. Thus, a potential limitation is that these data may not be representative of the general university population. It is possible that college students who are more physically active and/or health conscious will enroll in health related courses due to their interest in the topic. As a result, the purpose of this section is to review the physical activity literature in college students enrolled in a health related course separately from the studies conducted in the general student population. To our knowledge, a total of seven studies assessing physical activity levels in college students enrolled in an activity based class, all of which were included in this section. Project GRAD (Graduate Ready for Activity Daily) '4’ 6’ was developed to promote the adoption and maintenance of physical activity among young adults transitioning from the university into adult roles. Three hundred and thirty eight college seniors from a large urban university were randomized into a course designed to promote physical activity or a control course which covered general health topics. Each subject completed a 7-day physical activity recall which assessed total energy expenditure, minutes per week of moderate exercise, and minutes per week of vigorous exercise at the beginning and end of the 15-week classroom based intervention. The classroom based intervention had no significant effects on total leisure time physical activity participation in men. However, women showed improvement in total leisure time physical activity, flexibility exercises, and strengthening exercises 6'. In addition, there were no significant intervention effects on physical activity in either men or women two years following the intervention '4. 31 Anding and colleagues 6 assessed physical activity levels in a convenience sample of 60 college women. Study participants were enrolled in one of three aerobics courses at a university which requires students to complete one semester of an activity related course. Students completed the Self Report Physical Activity Scale (SRPA) to indicate their physical activity level during the past month on a scale from 0 (avoiding physical activity entirely) to 7 (exercising more than 10 hours per week). Based on the results of the SRPA, only 33% of the sample was considered physically active. However, because these study participants were enrolled in an aerobics course, it is possible they wanted to increase their physical activity levels. Physical activity, fitness, body composition, muscular strength, and flexibility were assessed in 115 male and 143 female college students enrolled in a general wellness course emphasizing the importance of physical activity and fitness 56. Physical activity was evaluated via questionnaire by classifying activities into one of three categories: 1) aerobic, 2) recreational, and 3) weight training. Students who participated in at least two sessions of a category per week were considered a participant within that category. The results for physical activity patterns indicated the proportion of females likely to participate in aerobic training (78%) was significantly higher than males (34%). However, a significantly greater proportion of males (34%) were more likely to participate in weight training than females (18%). In addition, males (28%) were also more likely to participate in recreational activities than females (12%). A total of 274 male and female college students enrolled in a general health course were asked to complete a survey regarding their personal health choices. Physical activity participation was included in the assessment 5 1. Results of the study illustrated 32 that only 40% of males and 32% of females reported maintaining a regular physical activity schedule. Study participants were also asked to report the number of days in which physical activity participation was sufficiently intense to significantly increase heart rate for 20 minutes or more. Males reported significantly more days (3.9) of exercise that increased heart rate compared to females (3 .2). The authors concluded that a large proportion of college students, especially females, are not physically active. Brevard and associates '2 examined the impact of residence on physical activity in 104 college students enrolled in an introductory nutrition class. A self-report questionnaire was used to assess both residence and physical activity within this sample. Residence was assessed by categorizing subjects as either living on-campus or living off- campus. Physical activity levels (sedentary, lightly active, active, very active), mode (aerobic, anaerobic, combination, no exercise), and weekly caloric expenditure (0, 1-399, 400-899, 900-1499, 100-2499, >2500 kcal/week) were determined for each subject. Results of the study showed that activity level and mode of exercise did not differ between students living on-campus and off-campus. Average weekly caloric expenditure was lower in males living on-campus (4881 kcal/week) when compared to males living off-campus (6281 kcal/week). In contrast, average weekly caloric expenditure was higher in women living on-campus (2173 kcal/week) compared to those living off- campus (1420 kcal/week). However, these differences were not significant. The majority of physical activity data in college students has been collected in predominantly Caucasian samples and is therefore may not be generalizable to the African American college student population. Ford and Goode 23 assessed daily physical activity levels in African American college students. Study Participant included 224 33 undergraduate students enrolled in a health education class at a predominantly African American institution. Nearly 55% of the sample did not participate in daily physical activity. Of the 45% of the sample that participated in daily physical activity, 60% were males. Physical activity levels were examined in 254 African American college students enrolled in a personal health class 37. Physical activity was assessed via the Lipid Research Clinics Physical Activity Questionnaire (LRCPAQ) which assessed physical activity during work, physical activity outside of work, and the frequency of participation in strenuous activity. Based on a combination of responses, subjects were classified as very low active, low active, moderately active, or high active. Results showed that physical inactivity was significantly different between males and females. In fact, according to the LRCPAQ classifications, 65% of females were classified as either low active or vary low active compared to 42% in males. The results suggest a large proportion of African American college students, especially females, are not physically active 37. In addition, study participants were asked to complete the Physical Activity History Questionnaire (PAHQ) which assesses participation in 13 specific vigorous and moderate intensity activities. Results fi'om the PAHQ suggested males participate most frequently in: 1) high intensity home and leisure activities such as weight lifting and 2) strenuous sports such as basketball, football, and skiing. For moderate intensity activities, males were most likely to report participation in walking and non-strenuous sports such as softball, volleyball, and swimming. Within the female population, rtmning and exercise dancing were reported most frequently for high intensity activities while walking and home exercises were reported most frequently for moderate activities. 34 These results offer a more detailed perspective on the type of physical activity among African American college students 37. To our knowledge, these are the only two studies which have focused only on Afi'ican American students. These results illustrated that 42-65% of all African American college students were physically inactive, which is similar to the 42% to 56% 26’ 32‘ 44’ 48’ 57 that found in the general college student population. To firrther assess ethic disparities in physical activity levels in college students, Suminski and colleagues 67 reported that physical activity participation (global self-rating of physical activity participation during the previous month) did not differ between Afiican Americans and Caucasians. Therefore, the current literature suggests that that physical activity participation in African American and Caucasian college students does not differ. As stated earlier, it is possible that a more health-conscious, physically active college student may be more likely to enroll in activity related course. As a result, their physical activity levels may be different from the general population of college students. However, no previous study has directly addressed whether this selection bias exists. Results from previous studies illustrate that the proportion of inactive college students enrolled in a health related course ranged from 42% to 77% which is similar to the 42% to 57% that found in the general college student population. Although these results suggest that physical activity may not differ between students who choose to enroll in an activity related course and those who do not, there is a need to assess this potential selection bias in more detail. 35 2.5 Decline in Physical Activity from High School into College Introduction The decline in physical activity with age is one of the most consistent findings in physical activity epidemiology 60. Childhood is the most physically active time during an individual’s life 63* 73. However, it appears the decline in physical activity begins during adolescence and continues to decline throughout a lifespan. Although it is accepted that physical activity declines with age, the reasons for this trend are not well understood 60. As a result, there is a need for a more detailed understanding of physical activity behaviors across a variety of age groups including young adults as they transition from high school to the university setting. The transition from high school to university is a complex phenomenon that has been considered more than a single event 24. This process appears to be a unique experience to each individual and is of substantial interest to educational professionals. The changes that occur during the transition fi'om high school to university can be academic, social, cultural, emotional, and physical 24. The purpose of this section of the literature review is to focus on this transition and assess changes in physical activity levels between high school and college. Currently, there is a lack of research on the change in physical activity from high school to college. The terms high school, college, university, physical activity, sport, exercise, adolescent, young adult, and transition were used in various combinations when searching the National Library of Medicine database. Following identification of all relevant articles, a careful examination of each literature review was conducted to identify any additional relevant studies that may have been overlooked. Our search yielded a total of twelve relevant studies which are included in 36 this section of the literature review. Two large population based studies were used to compare differences in the prevalence of physical activity in high school students and college students. To the best of our knowledge, only four studies focused Specifically on the Specific transition between high school and college while eight studies focused on longitudinal changes in physical activity from adolescence into young adulthood. Physical Activity during the Transition from High School to College The concern that physical activity declines fi'om high school to university is illustrated by comparing prevalence data between the National College Heath Risk Behavior Survey (N CHRBS) and the Youth Risk Behavior Survey (YRBS). In 1995, surveillance data on health risk behaviors of college students were collected as part of the NCHRBS. This survey showed that only 38% of university students participated in vigorous physical activity on three or more of the past seven days 20. Compared to the NCHRBS, the prevalence of vigorous physical activity was much higher (55%) in high school students who were part of the 1995 YRBS 35. These findings provide evidence of a decrease in physical activity as a student transitions from high school to the university. However, these results are limited in that data are derived fiom two separate cross- sectional populations using different survey techniques and methods. AS a result, there is a need for longitudinal data on physical activity levels during the transition from high school to college. In order to assess this transition period, Bray and Born ” compared vigorous physical activity levels between high school the first year of college in 145, 18 and 19 year old students enrolled in a university. High school physical activity levels were 37 assessed retrospectively. Participants who reported an average of three or more bouts of vigorous physical activity (any physical activity strenuous enough to cause one to break a sweat or breathe heavy) lasting 20 minutes or longer per week during the last 8 weeks of high school were classified as active. Similarly, those who participated in three or more bouts of vigorous physical activity lasting 20 minutes or longer per week during the first 8 weeks of college were classified as active. Individuals who reported less than three sessions of vigorous exercise lasting 20 minutes or longer were classified as insufficiently active. Results of the study showed that fi'equency of physical activity declined from 3.3 sessions per week during high school to 2.7 sessions per week during college. Sixty six percent of the population was classified as active during the last two months of high school. The proportion of active students significantly dropped to 44% during the first 2 months of college. Furthermore, 33% of respondents were active continuously (active during high school and college), 23% were insufficiently active continuously (insufficiently activity during high school and college), 11% were insufficiently active during high school and became active during college, while 33% were active during high school and became inactive during college ”. These results illustrate a decline in physical activity as student’s transition from high school to the university. Bray '0 also examined barriers to physical activity as a predictor of physical activity levels during the transition form high school to college. A total of 127 first year students at a liberal arts university in Canada provided self reports of their physical activity for the eight months prior to enrolment in the university and the first year at the university. Results showed that physical activity tracked moderately (r=.58) between high school and college. Furthermore, the ability of college students to cope with 38 potential barriers to physical activity (lack of motivation, cold weather, demands both inside and outside of school, social barriers, etc.) predicted physical activity levels during this transition '0. To examine further the changes in physical activity participation during the transition from high school to college, 149 female and 46 male first-year university students enrolled in a health science course completed 7 day recall of vigorous physical activity 40. Average weekly caloric expenditure for the entire p0pulation was 27.8 kcal/kg/week and there were no significant gender differences. When asked about previous physical activity levels, 42% believed they were less active now compared to their last year of high school, 25% thought they performed similar amounts of vigorous physical activity, and 33% thought they engaged in more vigorous physical activity 40. Everhart and colleagues 2' assessed the impact of sport participation and physical education enrolhnent during high school on physical activity levels during college. Current physical activity levels were assessed via questionnaire in 201 college students enrolled in two southwestern universities. Number of days per week students participated in at least 30 minutes of moderate-to-vigorous physical activity, strength training, cardiovascular activities, team sports, aquatic participation, and abdominal exercise were assessed separately. Results illustrated that students enrolled in high school physical education did not report higher physical activity levels during college, but did report increased strength training participation. Individuals participating in high school athletics, with or without high school physical education, reported greater team sports participation and strength training during college than non-athletes enrolled in physical education 2'. 39 In conclusion, there are minimal data comparing physical activity behaviors between high school and college students. Physical activity appears to decline, but there is a need for a more detailed perspective on the changes in physical activity during this time period. Researchers should focus on changes in sport participation, leisure time participation, and caloric expenditure as students move from high school to college. Changes in Physical Activity between Adolescence and Young Adulthood As stated previously, there is minimal research that focuses specifically on the changes in physical activity during high school and college. The majority of studies focus on the overall decline in physical activity between adolescence and young adulthood. Caspersen et al. 15 examined physical activity patterns across adolescents, young adults, middle aged adults, and older adults. Data fi'om over 10,000 males and females between the ages of 12 and 21 participating in the 1992 Youth Risk Behavior Survey and over 43,000 males and females greater than 18 years participating in the 1991 National Health Interview Survey were used to assess physical inactivity, light to moderate activity, and vigorous activity. The prevalence of adolescent physical inactivity (no participation in vigorous or moderate physical activity) increased fi'om 6% at age 14 to 24% at age 20 for male and female subjects. The prevalence of reporting regular, sustained physical activity dropped from 40% to 24% in boys and fi'om 30% to 20% in girls between the ages of 12 and 17. Between the ages of 12 and 21, vigorous physical activity declined from 76% to 42% in males and from 66% to 28% in females and continued to decline during young adulthood. This was followed by a more stable pattern 40 of physical activity prevalence between the ages of 30 and 64. In conclusion, physical activity behaviors decrease throughout adolescence and into young adulthood. The Cardiovascular Risk in Young Finns Study is a longitudinal project developed to assess physical activity patterns over a 21 year period from childhood to adulthood in Finland. Baseline measures were gathered in 1980 on 2309 children and 8 69. A physical activity index was calculated by adolescents between the ages of 9 and 1 assessing, the fi'equency and intensity of leisure time physical activity, participation in sport club training, participation in competitive sports events, and participation in physical education class. Continuously active subjects were defined as those who were in the highest tertile of the physical activity index score over a three year period (1980 to 1983) and a six-year period (1980 to 1986). Continuously inactive subjects were defined as those who were in the lowest tertile of the physical activity index over the same time period. The outcome variable was membership in the highest tertile 21 years following baseline measures. Logistic regression analysis indicated that 15 and 18 year old males who were active at baseline were more likely to be physically active 21 years later (OR: 5.7). The odds ratios for male subjects who were continuously activity over a three year period compared to those who were continuously inactive was 11.8 while the odds ratios for subjects who were continuously active over a 6 year period was 19.2. For females who were ages 15 and 18 years at baseline, the odds ratio of being physically active 21 years later was 2.8 if active at baseline, 4.4 if continuously active over a three year period and 6.1 if continuously active over a six year period. Similar results were shown for both males and females who were 9 and 12 years old at baseline. These results illustrate that the probability of being active in adulthood is much higher if physical activity is 41 continuous during adolescence 69. A separate study using the Cardiovascular Risk in Young Finns Study tracked physical activity behaviors over a 9-year period (1980-1989) 68. There was a 57% decline in the frequency of physical activity among males and 28% decline among females over the 9 year period. Furthermore, the relative decline in moderate and vigorous activity (frequency and intensity of physical activity combined) was 55% in males and 20% in females 68. Matton and colleagues 46 assessed the tracking of physical activity from adolescence to adulthood in 138 females involved in the Leuven Longitudinal Study on Lifestyle, Fitness, and Health. The physical activity behaviors of 14-18 year old adolescent females were assessed via questionnaire that assessed sports participation, physical activity at school, and sports participation during leisure time. In adulthood (ages 37-43), physical activity was assessed by asking subjects to identify their three most important sports. Subjects were then asked to report both frequency and duration of each sport. During adolescence, girls who reported less than three hours of sports per week were considered inactive, while those who reported three or more hours of work were considered active. During adulthood, women reporting less than 1.5 hours of sports per week were considered inactive while those participating in 1.5 or more hours per week were considered active. During adolescence, 59% of girls were labeled active. Of the girls who were labeled active, only 54% remained active in adulthood. Of the 41% of adolescent girls who were labeled inactive, 63% remained inactive in adulthood. These results suggest that physical activity levels do not remain stable between adolescence and adulthood. 42 The National Longitudinal Study of Adolescent Health cohort is a nationally representative school based study of more than 14,000 diverse youth between grades 7 and 12 which assesses the longitudinal trends in a variety of health behaviors of these youth into young adulthood. Physical inactivity was assessed via questionnaire and was defined as those adolescents who reported engaging in no weekly physical activity. During adolescence, the prevalence of no weekly physical activity was 8% or less in each female racial group. During young adulthood, the prevalence of physical inactivity among different female racial groups increased between 31%-47%. For male adolescents, the prevalence of reporting no weekly physical activity was 5% or less in each racial group. This increased between 26%-3 0% during young adulthood. These findings suggest a decline in physical activity fi'om adolescence to young adulthood on ethnically diverse males and females 29. In a separate study using the National Longitudinal Study of Adolescent Health cohort, cluster analysis was used to identify meaningful patterns of physical activity during adolescence and how these patterns are associated with healthy activity levels during the transition to adulthood 49. Results identified seven different clusters of adolescents: 1) adolescents with a high frequency of television and video games, 2) adolescents who skateboard and play video games, 3) adolescents who play sports with their parents, 4) adolescents who use neighborhood recreation centers, 5) adolescents with parents who limit their television use, 6) adolescents who have control over their television use, but watch very little, and 7) adolescents with high participation in school activities. When compared to the television and video game group, the odds of meeting physical activity recommendations (five or more weekly bouts of moderate to vigorous 43 physical activity) were highest among the skating/skateboarding group (OR = 13.1), the play sports with parents group (OR = 5.8), the neighborhood recreation group (OR = 4.2), and the high participation in school activities group (OR = 4.3). During young adulthood, the odds of meeting physical activity recommendations declined in each cluster, but remained significantly higher in the skating group (OR = 1.77), the play sports with parents group (OR = 2.58), the neighborhood recreation group (OR = 2.26), and the high participation in school activities group (OR = 2.35). These results illustrate that within clusters of adolescents, physical activity patterns decline differently during the transition to adulthood 49. Tracking of physical activity between adolescence and young adulthood has also been studied using data from the Northern Ireland Young Hearts Program. During adolescence, daily participation 11 physical activity during a typical school day was assessed via self-report questionnaire. During young adulthood, habitual work activity, sports activity, and non-sports leisure activity were assessed 8’ 9. To assess the changes in physical activity from adolescence to young adulthood, Boreham and colleagues 8 collected data from 245 males and 231 females at age 15 and during young adulthood. Tracking of physical activity was assessed by dividing the subjects into three physical activity classes: the lowest 25%, the middle 50%, and the highest 25%. Tracking of these variables was assessed by determining the extent to which membership in a particular class was maintained into adulthood using a kappa value. In males, the tracking of physical activity was slight to fair (kappa = .202), but in females tracking was poor (kappa = .021). These results illustrate there is a decline in physical activity patterns during the transition form adolescence to young adulthood. In a separate study using the 44 Northern Ireland Young Hearts Project 9, the relationship between cardiovascular risk factor status during young adulthood and adolescent physical activity was compared. Although results showed no apparent relationships between physical activity during adolescence and risk status during young adulthood, increased fitness levels during adolescence were modestly associated with serum lipid levels and body fatness in young adulthood. Conclusion There are few studies assessing change in physical activity behaviors among high school and college students. In fact, the majority of research focuses on the general adolescent and young adult populations. These studies consistently show a decline in physical activity with age, but fail to target changes in physical activity levels that occur specifically in the high school and college populations. If a more detailed understanding of these changes can be established, it is possible to delay or prevent the decrease in physical activity that occurs during this time period. This may lead to increase the overall health and well-being of a college campus. 2.6 Changes in Physical Activity During College The decline in physical activity is well documented throughout childhood and adulthood 60. However, previous research illustrating what determines this decline is limited as it may be specific to certain periods of life. Therefore, it is important to relate the determinants of physical activity with certain age groups or focus specifically on distinct developmental transitions during a lifespan. Healthy Campus 2010, which 45 includes physical activity as a leading health indicator, was created by to establish national health objectives specifically for college students. The Healthy Campus 2010 document suggests that college students are an important population to study because it is a specific time period in which an individual’s life is in transition 5. Thus, it is important to focus on the determinants of physical activity during the time period when an individual is enrolled in college. Evidence suggests physical activity levels during the latter years of college may continue into adulthood. Sparling and Snow 65 showed that 85% of college graduates who were regular exercisers during their senior year of college were either as active or more active five to ten years following graduation. Although these results suggest that physical activity levels during the senior year of college may influence future physical activity levels, it fails to assess the changes in physical activity participation over the entire 4 years of enrolhnent in college. Pinto and colleagues 57 assessed longitudinal changes in physical activity during the beginning of college. Two hundred forty-two first-year college students enrolled in a private university completed a baseline survey during their first year of college. A follow-up survey was completed during the second year. Each study participant was asked to report the total number of minutes spent in either: 1) vigorous physical activity (exercise that made you sweat and breath hard) or 2) moderate physical activity (exercise that did not make you sweat and breath hard). At baseline, average nrinutes spent in vigorous activity (128 minutes) and moderate activity (97 minutes) were not significantly different than the follow-up values for vigorous (176 minutes) and moderate (104 minutes) activity 57. These results suggest longitudinal changes in physical activity 46 during college are minimal. However, the results are limited to the time period between the first and second year of college. Therefore, it is necessary to assess physical activity throughout the entire 4-year period of college to have a more global perspective on physical activity participation in college students. To the best of our knowledge, no studies have assessed the changes in physical activity and the corresponding determinants that result in these changes over four years of enrolhnent in college. Thus, our understanding of the influence of college enrollment on physical activity levels is limited and there is a need to develop research protocols to assess this relationship. The purpose of the third aim of this project is to develop a longitudinal research protocol which will track physical activity behavior and its determinants effectively over a four year college career. Due to a lack of longitudinal research on physical activity in college students, it is important to conduct new and innovative research in this area to obtain a clear assessment of changes that occur in physical activity during this period. If future research can better assess physical activity behaviors throughout enrolhnent in college, universities will be able to more effectively target those at risk for becoming physically inactive during college. As a result, physical activity behaviors among college students will improve and these behaviors may continue into adulthood. 47 CHAPTER 3 METHODS 3.1 Research Design In brief, the research for this dissertation started with an undergraduate student population at a large Midwestern University. Recruitment from a healthy lifestyles class student population and a communications class student population made it possible to compare these students cross-sectionally and after completion of their classes. Assessment of these students included an initial cross-sectional survey followed by a re- assessment at the end of the semester 15 weeks later and included analysis of cross- sectional (baseline), as well as analysis of the longitudinal data. The first cohort included students enrolled in a healthy lifestyles class recruited in September 2005 and followed through December 2005. The second cohort included students enrolled in a healthy lifestyles class recruited in January 2006 and followed through April 2006. The third cohort included students enrolled in a communications class recruited in September 2005 and followed through December 2005. While these students were not enrolled at random to their classes, one of the focal points of this research was to assess the impact of enrollment in a healthy lifestyles class on physical activity energy expenditure compared to students enrolled in a communications class. This research protocol had two major elements: 1) completion of an anonymous online survey with standardized survey questions and 2) a repetition of the online survey 15 weeks later. Figure 1 represents the timeline of this research protocol. 48 Figure 1. Timeline for data collection Cohort l Cohort 1 Cohort 2 Cohort 2 Healthy Lifestyles: Healthy Lifestyles: Healthy Lifestyles: Healthy Lifestyles: Baseline Survey F ollow-up Survey F ollow-up Survey F ollow-up Survey September December April 2006 2005 2005 Cohort 3 Cohort 3 Communications: Communications: Baseline Survey Follow-up Survey 3.2 Study Population The goal of this project was to determine if physical activity participation in college students was improved via classroom based educational interactions that occur during the college years. In addition, this project was designed to assess the impact of gender, class membership, and high school sport and physical activity participation on physical activity during college. Undergraduates enrolled in the healthy lifestyles class (KIN 121) were designated as the target population of primary interest because these students received educational interactions intended to influence their physical activity levels. All kinesiology majors are required to take KIN 121, however non-kinesiology majors are allowed to take the class as an elective. For a contrast with the experiences received during enrollment in the healthy lifestyles class, a second target population was identified as undergraduate students enrolled in a communications class (COM 225), which did not include the activity and health component of the healthy lifestyles class. All communication majors are required to take COM 225, however non-communication majors are allowed to take the class as an elective. For the first cohort (KIN 12], fall 2005), the total number of students enrolled was 286. For the second cohort (KIN 121, 49 winter 2006), the total number of students enrolled was 305. For the third cohort (COM 225, fall 2005), the total number of students enrolled was 590. The total number of students eligible to participate was 1240. 3.3 Eligibility Criteria (inclusion/exclusion) All students enrolled in the healthy lifestyles or communications classes were eligible for participation in this research. There were no exclusion criteria applied to these undergraduate enrollees, unless a student was enrolled in both healthy lifestyles and communications classes concurrently. Four individuals were enrolled in both classes concurrently and their results were not included in the analysis. 3.4 Study Participants Among the 1240 eligible to participate, a total of 911 (74%) completed the baseline assessment and 765 (62%) completed the follow-up assessment. By cohort, these numbers and proportions for baseline and follow-up were as followed: Cohort I (KIN 121, fall 2005): 242 of 286 (85%), 193 of 286 (67%); Cohort 11 (KIN 121, winter 2006): 213 of 305 (70%), 177 of 305 (58%); Cohort III (COM 225, fall 2005): 456 of 590 (77%), 395 of 590 (67%). 3.5 Recruitment Procedures Due to the fact that all data collection was anonymous, this study was granted exempt approval status by the biomedical institutional review board at Michigan State University. In brief, the steps of the recruitment process were as followed. 50 1) 2) 3) 4) 5) 6) 7) 8) Consent forms (Appendix A) were distributed to all eligible study participants enrolled in study during the first week of class. The consent form included a survey admission ticket and instructions needed to access the on-line survey which utilized the Longitudinal Surveillance Engine (LSE). See 3.7 for a detailed description of the LSE assessment protocol. Verbal instructions were given by a study investigator to help ensure understanding of how to access the online survey. Students agreeing to participate used their unique LSE admission tickets to complete the online assessment. Study participants were given 10 days to complete the baseline questionnaire. To encourage participation, a reminder e-mail was sent to all study participants on the 5th, 7‘“, and 9th days of the 10 day period. A second series of reminder e-mails were sent during the 15th week of the study period encouraging subjects to participate in the follow-up survey. Study participants were given 10 days to complete the follow-up questionnaire. A reminder e-mail was sent to all study participants on the 5th, 7‘“, and 9th days of the 10 day period. Upon completion of the baseline and follow-up surveys, students were eligible to receive a coupon for a free ice cream cone at the Dairy Store located at Michigan State University. 51 3.6 Over-Arching Conceptual Model The basic conceptual model for Study Aim 1 and 2 are depicted in Figures 2 and 3, respectively. At the right of the figures are the primary outcome variables of interest (e. g. college physical activity participation). At the left of the Figure 2 are the exposure variables that may influence the physical activity outcome variable. The primary exposure variables that were addressed in sub-aim 1 (gender, class) are shown at the top left of Figure 2. The primary exposure variables that were addressed in sub-aim 2 (high school sport participation, high school leisure time physical activity) are shown at the bottom left of Figure 2. All additional exposure variables shown in Figure 2 were included in the analyses. The arrows at the far left represent the interactions that were assessed. At the far left of Figure 3 are sub-sets of time invariant covariates (e.g. gender, class, etc.) that could influence these response variables either directly or indirectly. The time invariant covariates reflect exposure variables that do not change over time. Midway between the time-invariant covariates and the response variables are tirne-variant covariates of interest. The time-variant covariates can vary over time and it is possible that changes in these variables can influence change in physical activity participation. The arrows at the far left of Figure 3 represent the interactions that were assessed. 52 Figure 2: Over-arching conceptual model for Study Aim 1 Exposure Variables Outcome variable Sub-aim 1 Primary Exposure Variables Gender Class Additional Exposure Variables Age Race Major Physical activity Lrvmg status participation BMI Television viewing [\ Video game use Sub-aim 2 Primary Exposure Variables High school / sport participation High school LTPA participation 53 Figure 3: Over-arching conceptual model for Study Aim 2 Time invariant Time variant Primary outcome covariates covariates variables Primary Exposure Variable of Interest Class BMI \ . . Change in Televrsron physical activity Gender vrewrng participation Age Race ll‘k Video game [[117 use Major ll, . , j .l Lrvrng status 7 High school physical activity and sport participation 3.7 Assessment Protocol: This research project utilized the Longitudinal Survey Engine (LSE) which was recently developed at Michigan State University. The LSE is an intemet based survey engine that offers anonymity and privacy for exploring topics that might be too sensitive to effectively engage participants in scientifically meaningful disclosures. The LSE is designed for large sample longitudinal, research protocols. Under the LSE protocols, 54 participants log in anonymously to create their own userID and passwords, and complete an initial baseline survey. Thereafter, participants are invited to return for follow-up surveys using the previously created userID and passwords. The userID/password feature allows the investigator to monitor individual and group patterns of health and behaviors anonymously over time. The LSE can be menu-driven using major intemet browsers (Internet Explorer, Netscape, etc.). The main features of the LSE used in the current investigation are: a) b) Each study participant was provided online access to the LSE (http://lse.commtechlab.msu.edu/login.php) The LSE software used a unique survey ID number to track each individual survey protocol (e. g., an initial online baseline questionnaire or assessment followed by longitudinal assessments), and to track each participant's responses at baseline and over time. (The LSE built a unique ID number to track both the survey protocol and the individual participant. This ID number was called a 'coupon number.') Prior to completion of the initial baseline assessment, the designated participant accessed the LSE website, keyed in his or her LSE coupon number, and was prompted to create his or her own userID and password. This was followed by prompting questions to help the individual recall the userID and password if these were lost or forgotten (e. g., 'What is the first initial of your mother's maiden name (surname before marriage)?’ This approach was used so designated participants could log in anonymously without direct or indirect linkage between the participant's identity and the survey responses. 55 d) Thereafter, the web browser displayed a study disclosure statement which invited participation. The participant could key the NEXT button to proceed to complete the survey, or could log out at any time. The LSE was designed to allow the participant to terminate participation and/or to skip over a survey item. e) Survey elements used in this investigation included true/false, multiple choice, and short answer items. f) The LSE kept track of successive log-ins by designated participants, which allowed them to complete repeated measurements. Storage of the repeated measures occurred within a database that encoded the userlD and a date/time stamp. Number of logins and the interval between repeated measures were controlled by the investigator via options given by the LSE. All responses were stored within a protocol-specific EXCEL Spreadsheet on the LSE secure server. 3.8 Primary Outcome Variables College leisure time physical activity participation: At baseline, each study participant was asked if s/he participated in any leisure-time physical activities or exercises such as running, calisthenics, golf, gardening, walking, etc. during the past month. If the study participant answered yes, a series of three remaining physical activity questions were asked to assess the type, frequency (sessions per week), and the duration (average minutes per session) of activity. These questions are based on similar previously validated instruments " 3' 33 . If the study participant reported participating in a second or third activity, the same series of questions were repeated for each activity. The same series of questions were repeated at the end of the 15-week testing period. The 56 survey is shown in appendix B. To determine if expressing the outcome variable differently would alter the results, college physical activity was expressed three ways. First, physical activity was expressed as a continuous variable calculated as kilocalories expended per week indexed by body weight (kcal/kg/week). A metabolic equivalent value (MET) was assigned for each activity using the Compendium of Physical Activities 2. One MET is equivalent to one kcal/kg/hour. Therefore, using the MET values for each activity, the number of sessions per week for the activity, and the number of minutes per session, the total number of METS per hour were determined and extrapolated into weekly caloric expenditure (kcal/kg/week). Second, physical activity was expressed as an ordinal variable calculated as quartiles of kcal/kg/week (Quartile 1: 0-12.4 kcal/kg/week, Quartile 2: 12.5-25.4 kcal/kg/week, Quartile 3: 255-458 kcal/kg/week, Quartile 4: 45.9 or more kcal/kg/week). Third, physical activity was expressed as a three level categorical variable based on the ACSM recommendations for moderate and vigorous physical activity 4 (Inactive: reported no leisure time activity or did not meet the physical activity recommendations, Moderately Active: engaged in moderate physical activity for at least 30 minutes on five or more days per week , Vigorously Active: engaged in vigorous physical activity three or more days per week for 20 or more minutes per occasion). Change in college physical activity participation: In order to assess change in caloric expenditure during the 15-week study period, the difference between physical activity levels at baseline and follow-up were assessed (Study aim 2). To determine if expressing the outcome variable differently would alter the results, the change in physical activity was expressed three ways: 1) as a continuous variable (difference in 57 kcal/kg/week at the beginning of the semester and kcal/kg/week at the end of the semester), 2) as a categorical variable using the physical activity quartiles (Decline: study participants who moved to a lower physical activity quartile at the end of the semester, Stay the same: study participants who remained in the same quartile throughout the semester period, Increase: study participants who moved into a higher physical activity quartile), 3) as a categorical variable using physical activity recommendations (Decline: study participants who remained in the inactive category, decreased fiom vigorously active to inactive, or decreased from moderately active to inactive, Stay the same: study participants who remained in either the inactive, moderate, or vigorous category, or moved from the moderate to vigorous category or vigorous to moderate category, Increase: study participants who increased from the inactive category to either the moderate or vigorous category). 3.9 Definitions for Exposure Variables Gender: Gender was defined as a dichotomous variable (male/female). Class membership: Class membership was defined as a dichotomous variable (healthy lifestyles/communications). Age: Age was defined as a categorical variable (18 years, 19 years, 20 years, 21 years, 22 or more years) Race: Race was defined as a categorical variable (Caucasian, African American, Other (e.g., Hispanic, Asian, Pacific Islander, Native American)). 58 Living Status: Current living status was defined as a dichotomous variable (living on-campus, living off-campus). Data on living status were collected at the beginning and end of the semester. Living Status change: Change in living status during the study period was assessed as a categorical variable: students whose living status did not change (no change), students whose living status changed from living on-campus to living off- campus (on/off-campus), and students whose living status changed from living off- carnpus to living on-campus (off/on-campus). Major: Major was defined as a dichotomous variable (kinesiology/other). Body Mass Index: Data on self-reported weight 0g) and height (m) were collected and body mass index (kg/m2) was calculated. For analysis, BMI was specified as a categorical variable based on the Expert Panel on the Identification, Evaluation, and Treatment of Overweight and Obesity on Adults 52. Subjects with a BMI between 18.5 and 24.9 were considered normal weight, between 25 and 29.9 were overweight, and 30 or greater were considered obese. Less than 2% of the population had a BMI less than 18.5 and were included in the normal weight group. Body mass index data were collected at the beginning and end of the lS-week study period. BMI change: Change in BMI during the study period was calculated by taking the difference of BMI at the beginning and end of the semester (BMI at the end of the semester — BMI at the beginning semester). A three level categorical variable was used to describe change in BMI: increase (a increase in BMI during the semester), stay the same (BMI that did not change during the semester), decrease (a decrease in BM] during the semester). 59 Television viewing: Television viewing was categorized as an ordinal variable (less than 2 hours per day, 2 hours per day, 3 hours per day, 4 hours per day). Television viewing data were collected at the beginning and end of the 15-week study period. Television viewing change: The change in television viewing during the study period was calculated by taking the difference of the amount of television watched at the beginning and end of the semester (TV hours at the end of the semester - TV hours at the beginning semester). A three level categorical variable was used to describe change in TV hours: increase (a increase in TV hours during the semester), stay the same (TV hours that did not change during the semester), decrease (a decrease in TV hours during the semester). Video game use: Video game use was categorized as an ordinal variable (0 hours per day, 1 hour per day, 2 or more hours per day). Data on video game use was collected at the beginning and end of the 15-week study period. Video game use change: The change in video game use during the study period was calculated by taking the difference of the amount of video game use at the beginning and end of the semester (VG hours at the end of the semester — VG hours at the beginning semester). A three level categorical variable was used to describe change in VG hours: increase (a increase in VG hours during the semester), stay the same (VG hours that did not change during the semester), decrease (a decrease in VG hours during the semester). High school sports: Participation in school sponsored sport activity was assessed by asking each subject if s/he participated in any school sponsored and/or club Sports or activities (e. g. basketball, cheerleading, marching band, etc.) during high school. If the student participated in a school sponsored sport, s/he was first asked to identify each high 60 school sport in which s/he participated. This was followed by asking: 1) how many months per year, 2) the average number of sessions per week, and 3) the average minutes per session s/he participated in these school-sponsored sports combined. Using MET values for each physical activity, average weekly caloric expenditure (kcal/kg/week) for a sponsored high school sports was calculated. In this study, high school sport participation was defined as the number of high school sports played (0 sports, 1 sport, 2 sports, 3 sports, 4 or more sports). High school leisure time physical activity participation flrigh school LTPA): Participation in high school leisure time physical activity was assessed by asking if each subject participated in any non-school sponsored leisure-time physical activities or exercises (any activity that was not sponsored directly by the high school) such as nmning, recreational sports, gardening, etc. during high school. This was also followed by a series of questions asking: 1) months per year, 2) average number of sessions per week and 3) average minutes per session s/he participated in these non school-sponsored physical activities combined. Using MET values for each physical activity, study participants were categorized as: 1) sedentary (reported no non-school sponsored sport activity, 2) inactive (reported some leisure time activity but did not meet the recommendations for moderate or vigorous activity), 3) moderately active (engaged in moderate physical activity (3-6 METS) for at least 30 minutes on five or more days per week) or 4) vigorously active (engaged in vigorous physical activity (greater then 6 METS)three or more days per week for 20 or more nrinutes per occasion). 61 3.10 Study Aim 1 The overall purpose of Study Aim 1 was to examine leisure time physical activity participation in college students. This aim had two sub-aims. Sub-aim 1 described college students’ current physical activity levels in relation to class membership (healthy lifestyles class/communications class) and gender. Sub-aim 2 assessed impact of participation in high school sponsored sports and non-high school sponsored sports and activities on physical activity participation during college. An underlying objective of Study Aim 1 was to determine if using different physical activity outcome variables would alter the results of our analyses. Therefore, physical activity was assessed in three different ways: 1) caloric expenditure (i.e., continuous), 2) quartiles for physical caloric expenditure (i.e., ordinal), and 3) meeting national recommendations for vigorous or moderate physical activity (i.e., categorical). 3.11 Statistical Analysis for Study Aim 1: In order to assess the impact of class membership on college physical activity participation, statistical power was determined assuming that data would be available from 450 students enrolled in the healthy lifestyles and 450 students enrolled in the communications classes, leisure time physical activity (LTPA) specified as kcal/kg/week was the outcome variable, and a 20% difference in physical activity is clinically meaningful. Using BRFSS data from Michigan used in a previous study (personal communication, Pivamik), LTPA energy expenditure averaged 24.0 kcal/kg/wk in 18-22 year-old respondents with a standard deviation of 25.4. Therefore, the following assumptions were used to calculate statistical power: 1) average LTPA for the healthy 62 lifestyles class is 25.0125 kcal/kg/wk, 2) average LTPA for the communications class is 20.0:t25 kcal/kg/wk, and 3) a two-tailed test with an alpha level of 5%. Statistical power for detecting a difference of 5 kcal/kg/wk is 0.85. All analyses were completed using SPSS 14.0. The first step in the analysis was to ensure a high quality dataset free of miscoded or erroneously missing values. Frequency distributions were assessed which disclosed the need for any recoding or transformations in advance of statistical analysis. After examination of the distribution of the continuous physical activity outcome variable (kcal/kg/week), it was determined that the variable was positively skewed and a transformation was needed. The log transformation of the kcal/kg/week variable normalized the data and was used in all analysis. The results of this transformation are shown in Appendix C. Initial descriptive analysis of the continuous outcome variable included one way- AN OVA. This approach was used to assess statistical differences (p<.05) in leisure time physical activity caloric expenditure (log of kcal/kg/min) for each categorical exposure variable (gender age, race, living status, major, body mass index, television, viewing, and video game use) at baseline. A linear regression model was used to assess the impact of class membership and gender on the log of kcal/kg/min after adjustment for age, race, living status, major, body mass index, television, viewing, video game use, and high school physical activity and sport participation. All exposure variables were entered into the model regardless of p-value in a backwards stepwise fashion. The variables were removed one at a time based on the largest p-value. Any exposure variable with a p- value <.05 was included in the final model. The following interactions were assessed in this and all subsequent analyses for Study Aim 1: gender/class, gender/age, gender/high 63 school sport, gender/high school LTPA, class/age, class/major, class/race, high school sport/high school LTPA, and television viewing/video game use. Ordinal logistic regression is used for analysis of data on an ordinal scale as it captures the ranked nature of the dependent variable. In this analysis, quartiles of physical activity is the ordinal outcome variable of interest. Prior to the regression analysis, cross-tabulations were generated and the chi square test for linear trend was used to determine any significant association (p<.05) between physical activity quartile and the exposure variables of interest. Using ordinal logistic regression, unadjusted odds ratios were calculated to determine the impact of each covariate on membership to a higher physical activity quartile. That is, this odds ratio applies to any adjacent quartile (i.e., Q1 compared to Q2-4, Q1-2 compared to Q3-4, or Q1-3 compared to Q4) and is the common odds ratio across each dichotomization. A positive odds ratio indicates an increased likelihood that a specific response to an exposure variable (e.g., male) will be observed in a higher category. A negative coefficient indicates a decreased likelihood that a Specific response to an exposure variable will be observed in a lower category. Using the ordinal logistic regression model, adjusted odds ratios were calculated to determine the impact of each covariate on membership to any higher physical activity quartile after adjusting for covariates. Each covariate was entered into the model and order of entry was dictated by the level of the p-value for the exposure variable. The underlying assumption of ordinal logistic regression is that the coefficients or odds ratios that describe the relationship between categories of the response variable are the same (proportional odds assumption). As a result, only one model (one odds ratio) is needed to describe each covariate in the model. In SPSS, the test of parallel lines is used to assess 64 the assumption of proportional odds. A p-value < .05 suggests that the model does not meet the proportional odds assumption. In the event that the proportional odds assumption was not met, the entire model was replaced and a multinomial logistic regression was used to analyze the data for all variables. Multinorrrial logistic regression was used to assess the three level categorical variable for meeting the recommendations for physical activity (i.e., inactive, moderately active, vigorously active). Cross-tabulations were calculated and chi square was used to determine statistical associations between meeting the recommendations for physical activity and the exposure variables of interest. Using multinomial logistic regression, unadjusted odds ratios were calculated to determine the impact of each covariate on meeting either the recommendations for moderate physical activity or vigorous physical activity. The inactive category served as the referent group. Adjusted odds ratios were then generated using multinomial logistic regression. Due to the small number of exposure variables, all were entered into the model. Gender, age, and race, were forced into the model while class, major, living status, television viewing, video game use, BMI, high school sponsored sport participation, and non-high school sponsored LTPA were entered regardless of level of significance in a backwards stepwise fashion. The variables not forced into the model were removed one at a time based on the largest p-value. Any exposure variable with a p-value <.05 was included in the final model. The interactions between the exposure variables previously described were assessed. 65 3.12 Study Aim 2 The overall purpose of Study Aim 2 was to assess the impact of college students’ enrollment in a semester long healthy lifestyles class on change in physical activity participation. Physical activity participation was assessed three ways: 1) change in caloric expenditure (kcal/kg/week) during the semester, 2) change in quartile of physical caloric expenditure during the semester, and 3) change in meeting the national recommendations for physical activity during the semester. 3.13 Statistical Analysis for Study Aim 2: In order to assess the impact of college students’ enrollment in a semester long healthy lifestyles class on physical activity participation, statistical power was determined assuming that: 1) complete data (two surveys over three months) would be available fiorn 250 students, and that 2) leisure time physical activity (LTPA) specified as kcal/kg/week is the primary endpoint, and 3) a 20% increase is clinically meaningful. Using BRF SS data from Michigan in a previous study (personal communication, Pivarnik), LTPA energy expenditure averaged 24.0 kcal/kg/wk in 18-22 year-old respondents with a standard deviation of 25.4. Thus, the statistical power for detecting an increase of 5 kcaI/kg/wk (two-tailed test with an alpha level of 5%) was 0.88. All analyses were completed using SPSS 14.0. The same procedures shown in section 3.11 were used to: 1) ensure a high quality dataset and 2) assess the frequency distributions. The change in log kcal/kg/week was used in analysis of the continuous variable. One way-ANOVA was used to assess significant differences (p<.05) in the change in caloric expenditure over a semester, calculated as the difference between 66 beginning and end of semester kcal/kg/week (follow-up kcal/kg/week — baseline kcal/kg/week) , for class membership, gender, age, race, living status, major, body mass index, television, viewing, and video game use. A multiple linear regression model was used to assess the impact of class membership on change in log transformed caloric expenditure when specified as a continuous variable. The time invariant covariates class, gender, age, race, living status, and major, and time variant covariate body mass index, television, viewing, and video game use were entered into the model in a backwards stepwise fashion. All exposure variables were entered into the model regardless of p- value in a backwards stepwise fashion. The variables were removed one at a time based on the largest p-value. Any exposure variable with a p-value <.05 was included in the final model. The time invariant and variant covariates were not handled differently in the model. The following interactions were assessed in this and all subsequent analyses for Study Aim 2: gender/class, gender/age, class/age, class/major, class/race, and television viewing/video game use. Multinorrrial logistic regression was also used to assess the change in physical activity quartile and change in physical activity category during the semester. Cross- tabulations were calculated and the chi square was used to determine any significant association (p<.05) between these two variables and the exposure variables/covariates. Using multinomial logistic regression, unadjusted, bivariate odds ratios were calculated to assess the impact of each covariate on: 1) moving to a lower physical activity quartile or category, or 2) moving to a higher physical activity quartile or category during the semester. Those individuals who remained in the same physical activity quartile or category during the semester served as the referent group. Adjusted odds ratios were 67 then calculated to determine the impact of each covariate on change in physical activity quartile after adjustment for covariates. Gender, age, and race, were forced into the model while class, major, living status, television viewing, video game use, and BMI, were entered into the model in a backwards stepwise fashion regardless of level of significance. The variables not forced into the model were removed one at a time based on the largest p-value. Any exposure variable with a p-value <.05 was included in the final model. The interactions between the exposure variables previously described were assessed. 3.14 Supplementary Statistical Analysis A series of supplement analyses were performed to address the limitations of the statistical analyses previously described. The limitations are described in detail within this section and the results of the additional statistical analyses are reported in Chapter 4. The inability to account for the attrition rate in subject participation from the baseline to follow-up surveys was the first limitation in the prior modeling strategies. The previous analysis used a change score, which was calculated as the difference in physical activity energy expenditure between baseline and follow-up. Therefore, the analysis was restricted to those students who completed both the baseline and follow-up surveys and data were excluded from 146 subjects. An alternative to this method is to assess the impact of the enrollment in a healthy lifestyles class using a multivariate model which allows for an outcome response variable that contains both baseline and follow-up physical activity values. Although this analysis does not formally deal with the attrition between baseline and follow-up, it uses all available data at baseline to assess the contrast 68 in physical activity energy expenditure between students enrolled in the healthy lifestyles class and the communications class. This analysis allows us to test if the contrast in energy expenditure between the two classes at follow-up (which includes data only fiom students who completed the follow-up survey) is greater than the contrast at baseline (which includes data from all students who completed the baseline survey). There are two alternatives when conducting this analysis. The first alternative is to estimate the population-averaged estimates of the effect of the healthy lifestyles class on physical activity levels. Generalized linear models and generalized estimating equations were used to estimate this effect. This estimate formally tests if the magnitude of difference in physical activity between the healthy lifestyles and communications at the end of the semester is larger than the size of the difference between these two classes at baseline. The population-based approach estimates the effect of taking the entire sample and assigning them to either the healthy lifestyles class or the communications class. The second alternative is to estimate the subject-specific effect of the healthy lifestyles class on levels of physical activity. A random intercept version of the generalized linear model was used to estimate this effect. This method estimates how much improvement in physical activity a college student can receive if s/he were assigned to the healthy lifestyles class rather than the communications class. Stata 8.0 was used for all analyses. The value of the calculated change score was the second limitation that needed to be addressed. Change scores do not take into account imbalances at baseline between the students enrolled in the healthy lifestyles and communications classes. Because of regression to the mean, it is possible that students with lower baseline physical activity energy expenditure values are more likely to improve than those with higher values. An 69 alternative to the change score approach is to use the follow-up energy expenditure value as the outcome variable and assess the impact of the exposure variables such as gender and class while holding the baseline energy expenditure value constant. This analysis was completed using a general linear model. The third limitation to be addressed was the nested structure of the healthy lifestyles class. That is, there were Six different sections of healthy lifestyles classes during each semester. It is possible that students enrolled in a given section of the healthy lifestyles class are more alike than students enrolled in a different section of the healthy lifestyles class. As a result, it is important to determine if physical activity energy expenditure during enrollment in the healthy lifestyles class is unique to specific sections of the healthy lifestyles class. In order to address this limitation, a generalized linear model was used and students were clustered by healthy lifestyles section to determine if results changed when controlling for the nested structure of this class. A fourth limitation addressed was the use of the class membership variable that did not distinguish between students enrolled in the healthy lifestyles class during the fall semester and those enrolled in the healthy lifestyles class in the spring semester. It is possible that change in physical activity participation during the semester is different between students enrolled in these two classes. Generalized linear models and generalized estimating equations were conducted using a three level categorical variable for class membership that distinguished between students enrolled in the fall and spring healthy lifestyles classes. 70 CHAPTER 4 RESULTS 4.1 Descriptive Data Among the 1240 students eligible to participate, a total of 911 (74%) completed the baseline assessment and 765 (62%) completed the follow-up assessment. By cohort, these numbers and proportions were as follows: Cohort I (KIN 121, fall 2005): 242 of 286 (85%); Cohort 11 (KIN 121, winter 2006): 213 of 305 (70%); Cohort [11 (COM 225, fall 2005): 456 of 590 (77%). Due to the similarities between the two Healthy lifestyles cohorts, they were combined for all subsequent analyses. Descriptive data for the Fall 2005 and Spring 2006 healthy lifestyles classes are shown in Table 1 to illustrate these similarities. There were no statistically significant differences in physical activity, gender, race, living status, BMI, and high school physical activity participation. There were significant differences in age and major as the Spring 2006 healthy lifestyles class enrolled a lower proportion of 18 year olds and kinesiology majors. The change in physical activity energy expenditure (change in log kcal/kg/week) approached statistically significant differences between the two healthy lifestyles classes. On average, students enrolled in the fall semester decreased energy expenditure during the semester whereas the spring semester students did not. This difference is discussed in the supplementary analysis section (Section 4.14). 71 Table 1. Descriptive data comparison for the healthy lifestyles classes Fall 2005 Sprmg3006 n % n % Chi-square p-value Age 22 years or more 36 14.9 29 13.6 0.002 21 years 43 17.8 45 21.1 20 years 48 19.8 46 21.6 19 years 42 '1 7.4 60 28.2 18 years 73 30.2 33 15.5 Gender Male 86 35.5 61 28.6 0.132 Female 156 64.5 152 71.4 Race Afiican American 22 9.1 25 11.7 0.286 Other 26 10.7 15 7.0 Caucasian 194 80.2 173 81.2 Major Kinesiology 121 50.4 68 32.2 <.001 Other 1 19 49.6 143 67.8 Living Status On-campus 122 5 1 .3 100 47.2 0.397 Off-campus l 16 48.7 1 12 52.8 BMl Obese 13 5.5 9 4.3 0.744 Overweight 53 22.5 52 24.6 Normal weight 170 72.0 150 71.1 High school sports 4 or more 36 14.9 30 14.1 0.616 3 sports 65 26.9 53 24.9 2 sports 60 24.8 50 23.5 1 sport 39 16.1 47 22.1 0 sports 42 17.4 33 15.5 High school LTPA Vigorous 84 34.7 65 30.7 0.377 Moderate 22 9. l 19 9.0 Inactive 62 25.6 70 27.4 Sedentary 74 30.6 58 46.7 Physical activity quartile 0-12.5 kcang/week 49 20.2 45 21.4 0.503 12.5 - 25.5 kcal/kg/wk 53 21.9 53 25.2 25.5 - 45.9 kcal/kg/wk 61 25.2 57 27.1 >45.9 kcal/kg/wk 79 32.6 55 26.2 Physical activity recommendations Inactive 35 14.5 38 18.0 0.268 Moderate 36 14.9 22 10.4 Vigorous 171 70.7 151 71.6 mean SD mean SD p-value Log kcaI/kg/wk 3.40 0.91 3.25 0.88 0.273 Chflge in Iowawgwk -0.15 0.74 0.02 0.78 0.050 72 Descriptive data for age, gender, race, major, living status, and BMI category are shown in Table 2. Chi square analysis was used to assess the statistical differences between the healthy lifestyles and communications classes. The majority of the sample (73%) was between the ages of 18 and 20 years, female (65%), Caucasian (84%), and normal weight (72%). An even proportion of students lived on-campus and off-campus. Nearly 41% of students enrolled in the healthy lifestyles class were kinesiology majors compared to only 1% of the students enrolled in the communications class. After formally testing if physical activity levels were different between the kinesiology majors and non-kinesiology majors, our results showed that physical activity levels in kinesiology majors (46. 1:8 7.5 ) were significantly higher (p<.05) than the non-majors (31.6:|:28.2) enrolled in the healthy lifestyles class. 73 Table 2: Descriptive data for the study population Healthy Chi- Total Lifestyles Communications square (n=9l 1) (n=45 5) (n=456) p-value n % n % n % Age 22 years or more 101 11.1 65 14.3 36 7.9 <.001 21 years 143 15.7 88 19.3 55 12.1 20 years 185 20.3 94 20.7 91 20.0 19 years 257 28.2 102 22.4 155 34.0 18 years 225 24.7 106 23.3 119 26.1 Gender Male 321 35.2 147 32.3 174 38.2 0.038 Female 590 64.8 308 67.7 282 61.8 Race Afiican American 71 7.8 47 10.3 24 5.3 0.014 Other 79 8.7 41 9.0 38 8.3 Caucasian 761 83.5 367 80.7 394 86.4 Major Kinesiology 193 21.2 189 41.5 4 0.9 <.001 Other 714 78.4 262 57.6 452 99.1 Living Status On-campus 439 48.2 222 48.8 217 47.6 0.323 Off-campus 467 51.3 228 50.1 239 52.4 BMI Obese 50 5.5 22 4.8 28 6.1 0.052 Overweight 181 19.9 105 23.1 76 16.7 Normal weight 659 72.3 320 70.3 339 74.3 4.2 Description of Study Aim 1 The first Study Aim assessed the effect of gender, class membership, and high school physical activity and sport participation on physical activity levels in college. An underlying objective of Study Aim 1 was to assess if the impact of these exposure variables change when using different physical activity outcome measures. Therefore, Study Aim 1 utilized a continuous variable (log transformed kcal/kg/wk), ordinal variable (quartiles of kcal/kg/wk), and categorical variable (meeting the recommendations for 74 moderate or vigorous physical activity) for all analyses. Specifically, Study Aim 1 for this dissertation was: Study Aim 1 (sub-aim 1): Does gender or class membership influence physical activity energy expenditure in college students? Study Aim 1 (sub-aim 2): Does high school sport and physical activity participation influence physical activity energy expenditure in college students? 4.3 Assessment of Physical Activity Outcome Variable It was determined that results remained essentially the same regardless of how physical activity was specified. That is, the impact of the exposure variables on physical activity participation during college was similar whether using the continuous, categorical, or ordinal physical activity outcome variable. Therefore, we chose to use the models based on the ordinal outcome, physical activity quartiles, due to its ease of interpretation. Physical activity quartiles were created based on the physical activity caloric expenditure results (kcal/kg/wk). Figure 4 Shows the fi'equency distribution and resultant quartiles for caloric expenditure in college students at entry into the cohort. Quartile 1 (Q1) corresponds to a caloric expenditure between 0 and 12.5 kcal/kg/wk, quartile 2 (Q2) between 12.5 and 25.5 kcal/kg/wk, quartile 3 (Q3) between 25.5 and 45.9 kcal/kg/wk, and quartile 4 (Q4) 45.9 or more kcal/kg/wk. All subsequent analyses in this project will use this ordinal physical activity outcome variable as well. The results for the continuous physical activity outcome variable and the categorical physical activity outcome variable are shown in Appendix D and Appendix B, respectively. 75 Figure 4: Graphical representation of physical activity quartile data won or 02 03 Q4 801 3 60~ H E '1 3 6' O L. IL 40‘! 1 20d :— 0-—- -- ~ ~ ~. ~ 0.00 50.00 1001!) 150.00 kcallkglwook 4.4 Study Aim 1 Descriptive Results The chi square test for linear trend was used to assess the linear relationship between each predictor variable and quartile of physical activity at the beginning of the semester (Table 3). There are statistically significant linear relationships between quartile of physical activity and gender, class membership, major, race, age, living status, television viewing, video game use, number of high school sponsored sports played, and participation in non-sponsored high school physical activity. These statistically significant trends illustrate that physical activity was higher in males, students enrolled in 76 the healthy lifestyles class, kinesiology majors, Caucasians, and those who were more active in high school sponsored sports and high school LTPA. Physical activity was lower in older students and those who watched more television. 77 Table 3. Descriptive data for quartiles of plysical activity at the beginning of a semester 0-12.5 12.5 - 25.5 25.5-45.9 >45.9 p-value kcal/kg/wk kcal/kg/wk kcal/kg/wk kcal/kg/wk for Variable N N % N % N % N % trend Gender Male 321 43 13% 77 24% 76 24% 125 39% 0.000 Female 586 184 31% 152 26% 149 25% 101 17% Class Healthy Lifestyles 452 94 21% 106 23% 118 26% 134 30% 0.000 Communications 455 133 29% 123 27% 107 24% 92 20% Major Kinesiology 193 25 13% 42 22% 50 26% 76 39% 0.000 Other 710 202 28% 186 26% 1 73 24% 149 21% Race African American 71 38 54% 9 13% 7 10% 17 24% 0.000 Other 78 26 33% 23 29% 12 12% 17 22% Caucasian 759 163 21% 197 26% 206 27% 192 25% Age 22 orolder 101 29 29% 33 33% 21 21% 18 18% 0.003 21 141 39 28% 38 27% 31 22% 33 23% 20 185 44 24% 45 24% 53 29% 43 23% 1 9 25 5 67 26% 66 26% 62 24% 60 24% 18 225 48 21% 47 21% 58 26% 72 32% Living Status On-campus 437 106 24% 97 22% 110 25% 124 28% 0.035 Off-campus 465 121 26% 129 28% 1 14 25% 101 22% Television viewing 4 or more hours 71 24 34% 17 24% 14 20% 16 23% 0.024 3 hours 118 36 31% 29 25% 23 19% 30 25% 2 hours 236 60 25% 62 26% 60 25% 53 22% less than 2 hours 483 107 22% 121 25% 128 27% 127 26% Video game use 0 hours 639 178 28% 162 25% 164 26% 135 21% 0.000 1 hour or less 208 31 15% 54 26% 51 25% 72 35% 2 or more hours 60 18 30% 13 22% 10 17% 19 32% BM] Obese 49 15 31% 1 l 22% 12 24% l 1 22% 0.810 Overweight l 80 46 26% 47 26% 30 1 7% 57 32% Normal weight 657 162 25% 166 25% 181 28% 148 23% High school sports 4 or more 1 17 20 17% 26 22% 27 23% 44 38% 0.000 3 sports 222 46 21% 52 23% 63 28% 61 27% 2 sports 234 52 22% 67 29% 61 26% 54 23% 1 Sport 186 55 30% 50 27% 40 22% 41 22% 0 sports 148 54 36% 34 23% 34 23% 26 18% High school LTPA Vigorous 279 39 14% 51 18% 85 30% 104 37% 0.000 Moderate 75 9 12% 16 2 1% 18 24% 32 43% Inactive 276 78 28% 90 33% 68 25% 40 14% Sedentag 276 1 01 3 7% 72 26% 53 19% 50 1 8% 78 4.5 Test of the Proportional Odds Assumption. The underlying assumption of ordinal logistic regression is that the odds ratios describing the relationship between categories of the response variable are the same across quartiles of physical activity (proportional odds assumption). That is, the odds ratios for the variables in the equations would not vary significantly if they were estimated separately across different dichotomizations of the physical activity quartile data. As a result, only one model (one odds ratio) is needed to describe the data. In SPSS, the test of parallel lines is used to assess the assumption. A p-value <.05 indicates that the model does not meet the proportional odds assumption and a different model such as a multinomial logistic regression should be used to describe the data. Table 4 illustrates that gender, race, and BMI do not meet this assumption. As an example, a graphical representation of the proportional odds assumption is shown for race and class in Figures 5 and 6. In these analyses, quartile 1 was compared to quartiles 2-4, quartiles 1 and 2 were compared to quartiles 3 and 4, and quartiles 1-3 were compared to quartile 4. As shown in Figures 5 and 6, three separate bivariate logistic regression models were calculated for the assessment of race and gender. In the analysis of race, Caucasians were used as the referent category. Odds ratios varied from .24 to .91 in Afiican Americans. The fact that these odds ratios differ across cut points indicates that the proportional odds assumption was not met (Figure 5). In the analysis of class membership, the communication class was used as the referent category. Odds ratios varied minimally from 1.58 to 1.68 which illustrates the proportional odds assumption was met (Figure 6). 79 Figure 5. Graphical representation of the proportional odds assumption for the unadjusted analysis of race (African American vs. Caucasian) Odds Ratio 2.00 1.80 a 1.60 - 1.40 i 1.20 1.00 0.80 1 A 0.60 it * 0.40 r- - 0.20 0.00 Referent 02-4 to Qt 03-4 to 01-2 Q4 to 01-3 Physical Activity Category Figure 6. Graphical representation of the proportional odds assumption for the unadjusted analysis of class membership (Healthy Lifestyles vs. Communications) Odds Ratio 2.40 2.20 .. 2.00 - 1.80 tr 1.60 H 1.40 1.20 1.00 - 0.801- 0.60 Referent 1 02-4 to Q1 03-4 to Q1-2 Q4 to 01-3 Physical Activity Category 80 4.6 Study Aim 1 Bivariate Ordinal Logistic Regression Results To quantify the magnitude of the relationships between the exposure variables listed in section 3.9 and physical activity, we used bivariate ordinal logistic regression to generate unadjusted odds ratios for quartiles of physical activity at the beginning of a semester (Table 4). These results illustrate that the odds of being in a higher quartile of physical activity are 1.83 times greater in males compared to females. This odds ratio applies to any adjacent quartile (i.e., Q1 compared to Q2-4, Q1-2 compared to Q3-4, or Q1-3 compared to Q4) and is the universal odds ratio used to describe the magnitude of the relationship between gender and physical activity across each dichotomization. Subjects enrolled in the healthy lifestyles class have 62% higher odds of belonging to a higher quartile of physical activity than those enrolled in the communications class. Kinesiology majors are 71% more likely belong to a higher quartile of physical activity than non-kinesiology majors. African Americans have a 64% lower odds of belonging to a higher quartile of physical activity than Caucasians. When compared to 18 years olds, subjects 22 years of age or older were 48% less likely to belong to a higher quartile of physical activity, 21 year-olds were 36% less likely to belong to a higher quartile of physical activity, and 19 year-olds were 32% likely to belong to a higher quartile of physical activity. Students living on-campus were 29% more likely to belong to a higher quartile of physical activity. When compared to students who participated in no high school sponsored sports, students who participated in 4 or more sports were 2.7 times more likely to be in a higher physical activity category in college. Those playing 3 sports, 2 sports, or 1 sport during high school were 96%, 61%, and 28% more likely to be in a higher physical activity quartile during college. When compared to students who 81 were categorized as sedentary with respect to participation in high school LTPA, those who were categorized as vigorous were 3.4 times more likely to be in a higher physical activity quartile. Those who were categorized as moderate were 3.8 times more likely than the sedentary group to be in a higher physical activity category during college. As stated earlier, Table 4 includes the bivariate unadjusted odds ratios and the results for the proportional odds assumption when using ordinal logistic regression. Because gender, race, and BMI do not meet the proportional odds assumption, the final multivariate ordinal logistic regression model that addressed the relationship between the exposure variables also did not meet this assumption. As a result, a multinomial logistic regression was used in place of ordinal logistic regression to assess the relationship between the exposure variables and quartiles of physical activity for all subsequent analyses for Study Aim 1 and is described in more detail in section 4.6. 82 Table 4. Unadjusted ordinal logistic regression results for quartiles of physical activity at the beginning of a semester Variable OR 95% Cl's p-value Test for parallel lines Gender Male 1.83 1.57 2.12 0.000 0.030 Female 1.00 Class Healthy Lifestyles 1.62 1.28 2.05 0.000 0.954 Communications 1 .00 Major Kinesiology 1.71 1.43 2.03 0.000 0.643 Other 1.00 Race African American 0.36 0.23 0.56 0.000 0.000 Other 0.60 0.39 0.91 0.017 Caucasian l .00 Age 22 or older 0.52 0.34 0.79 0.002 0.838 21 0.64 0.44 0.93 0.020 20 0.75 0.53 1.07 0.112 19 0.68 0.49 0.94 0.020 18 1.00 Living Status On-campus 1.29 1.02 1.63 0.035 0.267 Off-campus 1 .00 Television viewing 4 or more hours 0.65 0.42 1.02 0.059 0.728 3 hours 0.75 0.53 1.08 0.126 2 hours 0.83 0.63 1.10 0.188 less than 2 hours 1.00 Video game use 0 hours 0.85 0.53 1.37 0.513 0.095 1 hour or less 1.59 0.95 2.66 0.078 2 or more hours 1.00 BMI Obese 0.86 0.51 1.44 0.564 0.019 Overweight 1.1 1 0.83 1.50 0.473 Normal weight 1.00 High school sports 4 or more 2.71 1.74 4.20 0.000 0.733 3 sports 1.96 1.35 2.86 0.000 2 sports 1.61 1.11 2.33 0.012 1 sport 1.28 0.87 1.88 0.218 0 sports 1.00 High school LTPA Vigorous 3.41 2.51 4.64 0.000 0.179 Moderate 3.87 2.42 6.20 0.000 Inactive 1.16 0.86 1.57 0.326 Sedentary 1.00 83 4.7 Study Aim 1 Bivariate Multinomial Logistic Regression Results A multinomial regression is an extension of bivariate logistic regression and is used when the outcome variable has three or more categories. In contrast to the ordinal logistic regression, multinomial logistic regression does not treat the outcome variable as ordinal. As a result, specific odds ratios are calculated to describe the magnitude of the relationship between the exposure variables and each category of the outcome variable. Table 5 illustrates the results for the unadjusted multinomial logistic regression. In this analysis, the lowest physical activity quartile was used as the referent group. The three variables that violated the proportional odds assumption of ordinal regression were gender, race, and age. The bivariate multinomial regression results for these three variables indicate that when compared to the lowest physical activity quartile, males had 2.2 higher odds of being in the second quartile than females, 2.2 higher odds of being in the third quartile, and 5.3 higher odds of being in the fourth quartile. When compared to Caucasians, African Americans were 80% less likely to be in the second quartile, 85% less likely to be in the third quartile, and 62% less likely to be in the fourth quartile. When compared to normal weight students, those who were overweight had the same odds of belonging to quartile 2, 42% lesser odds of belonging to quartile 3, and 37% greater odds of belonging to quartile 4. The bivariate multinomial logistic regression results for the remaining variables were as follows: Students enrolled in the healthy lifestyles class were 1.56 times more likely than students enrolled in the communications class to be in the third quartile and 2 times more likely to be in the fourth quartile. Kinesiology majors were 1.82 times more likely than non-majors to be in the second quartile, 2.3 times more likely to be in the third 84 quartile, and 2.5 times more likely to be in the fourth quartile. When compared to 18 year olds, students who are 22 years or older are 78% less likely, 21 year olds are 69% less likely, and 19 year olds are 64% less likely to be in the fourth quartile. When compared to participating in zero high school sponsored sports, those individuals participating in 4 or more sports were 2 times more likely to be in either the second or third quartile and 4 times more likely to be in the fourth quartile during college. Students participating in either two or three sports during high school were also more likely to belong in physical activity quartile 2, 3, or 4 during college. Compared to the lowest physical activity quartile, students who were vigorously active in non-sponsored high school physical activities were 1.83 times more likely to be in the second physical activity quartile, 4 times more likely to be in the third quartile, and 5.4 times more likely to be in the fourth quartile of physical activity during college. Students who were classified as moderately active during non sponsored high school activities were 2.5 times more likely to be in the second quartile, 3.8 times more likely to be in the third quartile, and 7 times more likely to be in the fourth quartile. The results Show that healthy lifestyles students were more likely than communication students to be physically active. In addition, kinesiology majors were more likely than non-kinesiology majors to be physically active. AS shown in Table 2, only four kinesiology majors were enrolled in the communications class compared to 189 in the healthy lifestyles class. Therefore, it is possible that differences in physical activity participation between classes are due to the large percentage of kinesiology majors enrolled in the healthy lifestyles class. In order to test for this possibility, unadjusted bivariate logistic regression was used to determine the magnitude of the association 85 between non-kinesiology majors enrolled in the healthy lifestyles class and the communications class. The results showed that when using the first quartile of physical activity as the referent group, the odds (95% CIs) belonging to quartile 2, 3, or 4 were similar (1.0 (66,151); 1.2 (.79, 1.83); 1.2 (80,190), respectively) between these two groups. In addition, when compared to non-kinesiology majors enrolled in the healthy lifestyles class, the odds of kinesiology majors belonging to quartiles 2, 3, and 4 were 1.9 (1.06, 3.57); 2.2 (1.22, 3.99); 3.55 (1.99, 6.31), respectively. This shows that the large number of kinesiology majors enrolled in the healthy lifestyles class was primarily responsible for differences in physical activity participation between students enrolled in the two classes at the beginning of the semester. Therefore, the class membership exposure variable was omitted when conducting the multinomial logistic regression. 86 Table 5. Unadjusted multinomial logistic regression results OR OR OR Q2 to Q3 to Q4 to Variable Q1 95 % CI Q1 95 % CI Q1 95 % CI Gender Male 2.17 1.41 3.33 2.18 1.42 3.36 5.30 3.47 8.08 Female 1 .00 1.00 1 .00 Class Healthy Lifestyles 1.22 0.84 1.77 1.56 1.08 2.26 2.06 1.42 3.00 Communications 1.00 1 .00 l .00 Major Kinesiology 1.82 1.07 3.1 1 2.34 1.39 3.93 4.12 2.50 6.79 Other 1.00 1.00 1.00 Race African American 0.20 0.09 0.42 0.15 0.06 0.33 0.38 0.21 0.70 Other 0.73 0.40 1.33 0.37 0.18 0.75 0.56 0.29 1.06 Caucasian 1 .00 1 .00 1.00 Age 22 or older 1.16 0.61 2.21 0.60 0.30 1.18 0.41 0.21 0.83 21 1.00 0.55 1.82 0.66 0.36 1.21 0.56 0.31 1.02 20 1.04 0.59 1.86 1.00 0.57 1.73 0.65 0.37 1.14 19 1.01 0.59 1.70 0.77 0.46 1.28 0.60 0.36 0.99 18 1.00 1.00 1.00 Living Status On-campus 0.86 0.59 1.24 1.10 0.76 1.59 1.40 0.97 2.03 Off-campus l .00 1.00 1 .00 Television viewing 4 or more hours 0.63 0.32 1.23 0.49 0.24 0.99 0.56 0.28 1.1 l 3 hours 0.71 0.41 1.24 0.53 0.30 0.96 0.70 0.41 1.22 2 hours 0.91 0.59 1.42 0.84 0.54 1.30 0.74 0.47 1.17 less than 2 hours 1.00 1.00 1.00 Video game use 0 hours 1.26 0.60 2.65 1.66 0.74 3.70 0.72 0.36 1.42 1 hour or less 2.41 1.04 5.58 2.96 1.21 7.23 2.20 1.02 4.75 2 or more hours 1.00 1.00 1.00 BMI Obese 0.72 0.32 1.60 0.72 0.33 1.57 0.80 0.36 1.80 Overweight 1.00 0.63 1.58 0.58 0.35 0.97 1.36 0.87 2.12 Normal weight 1 .00 1 .00 1.00 High school sports 4 or more 2.06 1.00 4.26 2.14 1.04 4.41 4.57 2.26 9.26 3 sports 1.80 1.00 3.22 2.18 1.23 3.86 2.75 1.50 5.04 2 sports 2.05 1.17 3.59 1.86 1.06 3.28 2.16 1.18 3.94 1 sport 1.44 0.81 2.57 1.16 0.64 2.09 1.55 0.83 2.87 0 sports 1.00 1.00 1.00 High school LTPA Vigorous 1.83 1.10 3.07 4.15 2.51 6.88 5.39 3.27 8.88 Moderate 2.49 1.04 5.96 3.81 1.60 9.07 7.18 3.18 16.20 Inactive 1.62 1.05 2.48 1.66 1.04 2.64 1.04 0.62 1.73 _ Sedentary 1.00 1.00 1.00 87 4.8 Study Aim 1 Adjusted Multinomial Logistic Regression Results Table 6 shows the final multivariate multinomial logistic regression model that addresses the relationship between gender, major, high school sponsored sport and LTPA participation and quartile of physical activity. Gender, race, age, and major were forced into the model. Television, video game use, BMI category, high school sport participation, and high school LTPA were entered stepwise into the model. Of these, only high school sport and high school LTPA remained significant and were retained. When compared to the first physical activity quartile, males had 2.5 greater odds of belonging to the second quartile than females, 2.6 greater odds of belonging to the third quartile, and 7.2 greater odds of belonging to the fourth quartile. After adjustment for the exposure variables, the odds of males being in higher physical activity quartiles remained greater than females. The odds of belonging to quartile 4 increased from 5.3 to 7.2 after adjustment. Figure 5 illustrates the cumulative change in odds ratios (quartile 4 to quartile 1) for gender following adjustment for the exposure variables included in the model. Students who were kinesiology majors were 2 times more likely than communications students to be in the second quartile, 2.4 times more likely to be in the third quartile and 4.2 times more likely to be in the fourth quartile. After adjustment for the exposure variables, the results for the impact of major on physical activity quartile were similar to the unadjusted analysis. Afiican American students were 79% less likely than Caucasians to be in the second quartile, 87% less likely to be in the third quartile, and 56% less likely to be in fourth quartile. When compared to 18 year olds, students who were 19 or 20 years of age were 55% less likely to be in the highest physical activity quartile. Students who were 22 years or older were 62% less likely to be in the highest 88 quartile. Students who participated in 4 or more high school sports were 2.2 times more likely to be in the second physical activity quartile, 2 times more likely to be in the third quartile and 5.2 times more likely to be in the highest quartile during college, compared to those who participated in zero high school sports. Those who participated in 3 Sports were 1.7, 2.1, and 3.1 times more likely to be in the second, third, and fourth quartile during college, respectively. Students who participated in 2 sports during high school were 2.3, 2.2, and 3.3 times more likely to be in the second, third, and fourth quartile during college, respectively. When compared to students who were classified as sedentary in high school LTPA, students who were vigorously active were 1.5 times more likely to be in the second quartile of physical activity, 3.1 times more likely to be in the third quartile, and 4.3 times more likely to be in the fourth quartile. Students who were classified as moderately active in LTPA were 2.5 times more likely to be in the second quartile of physical activity, 3.7 times more likely to be in the third quartile, and 9.9 times more likely to be in the fourth quartile. There were no significant interactions in the analysis. The global p—value represents the level of significance of the entire model. 89 Table 6. Adjusted multinomial logistic regression results for quartiles of physical activity at the beginning of a semester based on high school physical activity OR OR OR Q2 Q3 Q4 Global to to to p- Variable Q1 95 % CI Q1 95 % CI Q1 95 % CI value Gender <.001 Male 2.54 1.59 4.08 2.56 1.59 4.13 7.17 4.37 11.76 Female 1.00 Major Kinesiology 2.01 1.14 3.56 2.36 1.34 4.15 4.23 2.40 7.47 Other 1.00 1.00 Race Afiican American 0.21 0.09 0.47 0.17 0.07 0.43 0.44 0.21 0.96 Other 0.78 0.40 1.52 0.44 0.20 0.96 0.70 0.32 1.51 Caucasian 1 .00 1 .00 1 .00 Age 22 or older 1.35 0.66 2.76 0.73 0.34 1.57 0.38 0.16 0.88 21 years 1.13 0.59 2.13 0.73 0.38 1.42 0.72 0.37 1.43 20 years 0.84 0.46 1.56 0.86 0.47 1.56 0.46 0.24 0.88 19 years 0.89 0.51 1.54 0.69 0.40 1.20 0.44 0.24 0.79 18 years 1.00 1.00 1.00 High school sports 4 or more 2.17 0.99 4.73 1.97 0.89 4.36 5.24 2.29 11.98 3 sports 1.73 0.92 3.27 2.07 1.10 3.89 3.07 1.50 6.26 2 sports 2.31 1.25 4.25 2.17 1.16 4.04 3.33 1.64 6.76 1 sport 1.59 0.86 2.96 1.30 0.68 2.49 2.12 1.03 4.35 0 sports 1.00 1.00 1.00 High school LTPA Vigorous 1.54 0.90 2.66 3.14 1.84 5.35 4.31 2.46 7.55 Moderate 2.51 1.01 6.23 3 .74 1.52 9.22 9.88 4.02 24.27 Inactive 1.52 0.96 2.41 1.38 0.84 2.26 1.03 0.59 1.82 Sedentary 1 .00 1.00 1 .00 90 Figure 7. Change in odds ratios for gender after adjustment for exposure variables included in the adjusted multinomial logistic regression model 12 .. 111- ~- ------- - ~71» - 10,,, * ----'5~er- L- — a 71 L m 8 5. a 5,, e 4 L 3, L 2 ._. 1 r r Referent Gender ijor Race Age HS sports HS LTPA Variables Included in Adjusted Model 4.9 Description of Study Aim 2 The second Study Aim assessed the impact of college students’ enrolhnent in a semester long healthy lifestyles class on change in physical activity participation. AS explained in section 4.2, an underlying objective of this research was to assess if the impact of the exposure variables change when using different physical activity outcome measures. As we found in Study Aim 1, the results were similar when using the different physical activity outcome variables. Therefore, we also used the ordinal specification of physical activity (i.e., quartiles) in Study Aim 2 (see section 4.3). The research question for Study Aim 2 was: Study Aim 2: Does enrollment in a 3-month long healthy lifestyles class influence changes in physical activity energy expenditure? 91 4.10 Time Varying Covariates It is of interest to consider whether the impact of enrollment in the healthy lifestyles class on physical activity participation is different with respect to the time varying covariates (BMI, television viewing, video game use, living status change). To assess the change in these variables over the semester, differences from the baseline and follow-up surveys were calculated. Due to the brief duration of the study period, changes in BMI (.25i.85 units), television viewing (-.01i.96 hours) and video game use (.01i.59 hours) were negligible. Therefore, it is unlikely that the time varying covariates are contributing to change in physical activity. To test for this, we used three level categorical variables to assess the change in BMI, television viewing, and video game use in all subsequent analysis. Only 19 students reported a change in living status during the semester. AS a result, the baseline variable for living status was used in all subsequent analyses. These variables are described in more detail in section 3.3. 4.11 Study Aim 2 Descriptive Results Chi square analysis was used to assess the relationship with each predictor variable and the change in physical activity during enrolhnent in the healthy lifestyles class or communications class. Change in physical activity was defined as a three-level categorical variable: 1) Decline: participants who moved to a lower physical activity quartile or remained in the lowest physical activity quartile, 2) Stay the same: study participants who remained in the second, third, or fourth quartile throughout the semester period, 3) Increase: study participants who increased into a higher physical activity quartile. The descriptive results for change in physical activity quartile and their 92 associations with the predictor variables are shown in Table 7. There were no significant relationships between the time variant covariates and change in physical activity. Among the time invariant covariates, significant relationships were found between change in physical activity and class membership and major. These statistically significant associations illustrate that the percentage of students enrolled in the healthy lifestyles class who increased physical activity (23%) was greater than those who increased in the communications class (14%). The percentage of females who increased physical activity participation during the semester (21%) was higher than that of males (14%). In addition, physical activity energy expenditure decreased an average of 2.1 kcal/kg/week in the healthy lifestyles during the semester class compared to 6.7 kcal/kg/week in the communications class. When separating the two healthy lifestyles classes, physical activity energy expenditure decreased 4.3 kcal/kg/week among students enrolled during the fall semester and remained the same among students enrolled in the spring semester. The difference in change in physical activity energy expenditure between the two healthy lifestyles classes is addressed in supplemental statistical analyses (Section 4.14). 93 Table 7. Descriptive data for change in physical activity quartile stay the decrease same increase chi-square p- Variable N N % N % N % value Gender Males 255 97 38% 122 48% 36 14% 0.043 Females 508 160 31% 242 48% 106 21% Class Healthy Lifestyles 368 115 31% 168 46% 85 23% 0.008 Communications 395 142 36% 196 50% 57 14% Major Kinesiology 152 55 36% 67 44% 30 20% 0.593 Other 608 200 33% 296 49% 1 12 18% Race African American 46 9 20% 28 61% 9 20% 0.198 Other 63 18 29% 31 49% 14 12% Caucasian 654 230 35% 305 47% 1 19 18% Age 22 or older 79 20 25% 38 48% 21 27% 0.404 21 120 39 33% 60 50% 21 18% 20 159 51 32% 79 50% 29 18% 19 211 70 33% 104 49% 37 18% 18 194 77 40% 83 43% 34 18% Living Status On-campus 366 132 36% 167 46% 67 18% 0.3 72 Off-campus 393 123 31% 195 50% 75 19% Television viewing change Decrease 205 61 30% 103 50% 41 20% 0.517 Stay the same 346 121 35% 166 48% 59 17% Increase 1 91 5 8 30% 91 48% 42 22% Video game use change Decrease 83 20 24% 45 54% 18 22% 0.254 Stay the same 570 184 32% 276 48% 110 19% Increase 89 36 40% 39 44% 14 16% BM] Decrease 193 58 30% 97 50% 38 20% 0.881 Stay the same 149 45 30% 75 50% 29 19% Increase 367 124 34% 172 47% 71 19% 94 4.12 Study Aim 2 Unadjusted Bivariate Results A multinomial logistic regression was used to calculate the unadjusted odds ratios between exposure variables and change in physical activity across the semester (Table 8). When compared to individuals who remained in the same quartile of physical activity, males had 33% lower odds than females to increase physical activity quartile. Students enrolled in the healthy lifestyles class had 74% greater odds than students enrolled in the communications class to increase to a higher physical activity category. African American students had 57% lower odds than Caucasian students to decrease physical activity quartile during the semester. 95 Table 8. Unadjusted multinomial logistic regression results for change in quartile of physical activity during a semester. decrease vs. increase vs. stay the stay the Variable same 95 % CI same 95 % CI Gender Male 1.20 0.86 1.68 0.67 0.44 1.04 Female 1.00 1.00 Class Healthy Lifestyles 0.94 0.69 1.30 1.74 1.17 2.58 Communications 1 .00 1 .00 Major Kinesiology 1.21 0.82 1.81 1.18 0.73 1.92 Other 1.00 1.00 Race Afiican American 0.43 0.20 0.92 0.82 0.38 1.80 Other 0.77 0.42 1.41 1.16 0.59 2.25 Caucasian 1 .00 1 .00 Age 22 or older 0.57 0.30 1.06 1.35 0.69 2.63 21 0.70 0.42 1.17 0.85 0.45 1.62 20 0.70 0.44 1.11 0.90 0.50 1.61 19 0.73 0.47 1.12 0.87 0.50 1.50 18 1.00 1.00 Living Status On-campus 1.25 0.91 1.73 1.04 0.71 1.54 Off-campus 1 .00 l .00 Television viewing change Increase 0.87 0.58 1.31 1.30 0.81 2.08 Decrease 0.81 0.55 1.20 1.12 0.70 1.79 Stay the same 1.00 1.00 Video game use change Increase 1.38 0.85 2.26 0.90 0.47 1.72 Decrease 0.67 0.38 1.17 1.00 0.56 1.81 Stay the same 1.00 1.00 BMI Increase 1.20 0.78 1.86 1.07 0.64 1.78 Decrease 1.00 0.61 1.63 1.01 0.57 1.79 Staythe same 1.00 1.00 4.13 Study Aim 2 Adjusted Multinomial Regression Results Table 9 shows the adjusted odds ratios for change in quartile of physical activity during a semester enrollment in either a healthy lifestyles class or communications class. 96 Using multinomial logistic regression, gender, class, race, and age were forced into the model. The remaining time invariant covariates (living status and major) and time variant covariates (change in BMI, television viewing, and video game use) were entered stepwise into the model. Due to a lack of statistical significance these variables were not retained in the model. The results Showed that students enrolled in the healthy lifestyles class had 1.8] higher odds of increasing physical activity than the students enrolled in the communications class regardless of gender, age, race, and major. Our results also showed that the time varying covariates were not associated with changes in physical activity during the semester. After adjustment for class, age, and race males were 33% less likely than females to increase physical activity and 22 years olds were 49% less likely than 18 year olds to decrease their physical activity participation. There were no significant interactions within the exposure variables. It also should be noted that when compared to students who decreased physical activity, students enrolled in the healthy lifestyles class were 2.2 times more likely than communications students to increase physical activity. After stratifying our sample by class and major, our results also showed no differences in change in physical activity participation between kinesiology majors and non-majors enrolled in the healthy lifestyles class. In addition, non-majors enrolled in the healthy lifestyles class had 1.87 greater odds of increasing physical activity than non-maj ors enrolled in the communications class. These results imply that greater improvements in physical activity participation are seen in students enrolled in a healthy lifestyles class when compared to those who are not. 97 Table 9. Adjusted multinomial logistic regression results for change in quartile of physical activity during a semester decrease increase Global vs. stay the vs. stay the p- Variable same 95 % CI same 95 % CI value Gender 0.112 Male 1.18 0.83 1.70 0.67 0.42 1.06 Female 1 .00 1 .00 Class Healthy Lifestyles 0.89 0.63 1.27 1.81 1.20 2.73 Communications 1 .00 1 .00 Race African American 0.58 0.26 1.30 0.74 0.32 1.74 Other 0.65 0.32 1.32 1.11 0.54 2.28 Caucasian 1 .00 1.00 Age 22 or older 0.51 0.25 1.02 1.28 0.63 2.61 21 0.80 0.47 1.37 0.80 0.42 1.54 20 0.71 0.43 1.17 0.89 0.49 1.63 19 0.75 0.47 1.18 0.92 0.52 1.62 18 1.00 1.00 4.14 Results for Supplementary Statistical Analyses A series of supplementary analyses were performed to address the limitations of the statistical analyses previously described. The first limitation to the prior modeling strategies was the attrition in subject participation from the baseline survey to follow-up survey due to the use of a change score. As an alternative, a multivariate model which included all available physical activity energy expenditure data was used. Table 10 shows the results for the population based model assessing the impact of gender, time, and class on the log of physical activity energy expenditure throughout the 15-week study period. The results illustrate that males were more active than females, physical activity decreased over time, and that physical activity was greater in students enrolled in the healthy lifestyles class. These results show that the effect of class on physical activity 98 over time is not significant. This suggests the magnitude of the difference in physical activity at follow-up is not different than at baseline between students enrolled in the healthy lifestyles and communications classes. This contradicts our original results which suggest there was a difference between the two classes. In contrast to the population-based approach, a subject-specific modeling strategy assessing the impact of gender, time, and class on physical activity throughout the 15-week study period was also conducted (Table 11). The results did not differ between the population based and subject-specific approaches. Table 10. Estimated relationship between physical activity energy expenditure and class membership during enrollment in a healthy lifestyles class (population-based approach) Variable Coefficient 95% CI J Gender 0.456 0.345 0.565 <.001 Time -0.186 0.268 -0.103 <.001 Class 0.284 0.161 0.407 <.001 Class over Time 0.087 -0.028 3.130 0.139 Table 11. Estimated relationship between physical activity energy expenditure and class membership during enrollment in a healthy lifestyles class (subject-specific approach) Variable Coefficient 95% CI p Gender 0.456 0.345 0.568 <.001 Time -0.183 0.263 -0.103 <.001 Class 0.30] 0.191 0.426 <.001 Class over Time 0.095 -0.020 2.77 0.106 The second limitation addressed was the value of the calculated change score. An alternative to this approach is to use the follow-up energy expenditure value as the outcome variable and assess the impact of the exposure variables such as gender and class while holding the baseline energy expenditure value constant as shown in Table 12. The results show that after controlling for physical activity energy expenditure at 99 baseline, physical activity energy expenditure as follow-up was higher in students enrolled in the healthy lifestyles class. Similar to the original change score results, this analysis illustrates that students enrolled in the healthy lifestyles class are more likely to improve physical activity during the semester. Table 12. Estimated relationship between physical activity energy expenditure at follow- up and class membership after controlling for physical activity energy expenditure at baseline Change Score Coefficient 95% CI p-value Log kcal/kg/week at baseline 0.561 0.504 0.618 <.001 Gender (male) 0.089 -0.022 0.201 0.117 Class (healthy lifestyles) 0.141 0.081 0.201 <.001 The third limitation addressed was the potential differences that existed between different sections of the healthy lifestyles class. Specifically, students enrolled in the same section within the healthy lifestyles class are more alike when compared to students enrolled in a different section of the healthy lifestyles class. In order to address this limitation, a modeling approach that recognized the nested structure of the students within the healthy lifestyles sections was conducted and is shown in Table 13. These results illustrate that after controlling for physical activity participation at baseline and the nested structure of the healthy lifestyles class, physical activity participation at the end of the semester was higher in the students enrolled in the healthy lifestyles classes. These results are similar to the results shown in Table 11 which did not control for the nested structure of the healthy lifestyles class. 100 Table 13. Estimated relationship between physical activity energy expenditure at follow- up and class membership after controlling for physical activity energy expenditure at baseline and the nested structure of the healthy lifestyles class Change Score Coefficient 95% CI p-value Log kcal/kg/week at baseline 0.594 0.457 0.731 <.001 Gender (male) 0.105 -0.061 0.271 0.214 Class (healthy lifestyles) 0.145 0.006 0.283 0.041 A fourth limitation within the previous analyses was the use of the class membership variable that did not distinguish between students enrolled in the fall and spring healthy lifestyles classes. When analyzing the data using a 3-level categorical variable for class (fall communications, fall healthy lifestyles, spring healthy lifestyles), results indicate that: 1) males are more active than females, 2) students enrolled in either the fall or spring semester healthy lifestyles classes are more active than students enrolled in the communications class, 3) physical activity declines over time, 4) kinesiology majors are more active then non-majors, 5) Africa Americans are less active than Caucasians, and 6) those who watch more television are less active (Table 14). These results also show no difference in physical activity throughout the semester between the fall healthy lifestyles class and fall communications class. In contrast, when compared to the fall communications class, physical activity participation was more likely to increase in students enrolled in the spring healthy lifestyles class. Furthermore, physical activity participation in males decreases during the semester when compared to females. 101 Table 14. Estimated relationship between physical activity energy expenditure and enrollment in the fall or springhealthy lifestyles classes Variable Coefficient 95% CI p Gender Female 1 .000 Reference Male 0.510 0.376 0.644 <.001 Class Communications 1 .000 Reference Healthy Lifestyles Fall 0.244 0.075 0.025 0.005 Healthy Lifestyles Spring 0.233 0.074 0.392 0.004 Time Baseline l .000 Reference Follow-up -0.087 -0.194 0.019 0.108 Major Non-kinesiology major 1.000 Reference Kinesiology major 0.270 0.112 0.428 0.001 Age -0.045 -0.096 0.006 0.090 Race Caucasian 1 .000 Reference African American -0.419 -0.714 -0.123 0.005 Other -0.133 -0.371 0.105 0.273 Television viewing -0.072 -0.121 0.021 0.005 Video game use 0028 -0.103 0.047 0.468 Body Mass Index 0.009 -0.008 0.026 0.281 Living Status On-campus 1 .000 Reference Off-campus 0.066 -0.072 0.203 0.349 Class Fall over Time Communication 1 .000 Reference Healthy Lifestyles Fall -0.034 -0.176 0.108 0.638 Class Spring over Time Communication 1 .000 Reference Healthy Lifestyles Spring 0.159 0.019 0.296 0.026 Gender over Time Female 1 .000 Reference Male -0.126 -0.250 -0.003 0.045 102 CHAPTER 5 DISCUSSION 5.] Effect of Gender and Class on Physical Activity Participation The results of this study illustrate that both gender and academic major influenced physical activity participation in college students. We also found physical activity participation was lower in older college students and Afiican Americans and as hypothesized, higher in males. It was also hypothesized that physically active college students would be more likely to enroll in the healthy lifestyles class, resulting in selection bias. However, the higher physical activity participation of students enrolled in the healthy lifestyles class was due to the large number of kinesiology majors enrolled in that class rather than simply that physically active college students were more likely to enroll. The impact of gender on physical activity participation in college students has been studied previously, but has provided mixed results. Similar to our results , previous studies have shown that a larger proportion of male college students (45-49%) were more likely to report regular exercise than female students (35-39%) 48’ 65. Dinger and Waigandt '9 found that males participated on an average 2.8 days of vigorous physical activity per week compared to 2.2 days for females. However, other studies have found no difference in physical activity participation between males and females 44’ 58. A potential reason for the inconsistencies in these results is that physical activity was reported differently within each of these previous studies. That is, physical activity has been reported as the number of days per week of vigorous physical activity, the number of days of structured exercise, or as a categorical variable based on national physical 103 activity recommendations. Similar to our study, Leslie and associates 43 used a more detailed ordinal assessment of physical activity, which used frequency, intensity, and duration of physical activity to determine weekly physical activity energy expenditure (kcal/week). These authors also reported that females were more likely than males to be sedentary (<100 kcal/week) or low active (100-799 kcal/week), whereas males were more likely to be moderately active (>800 kcal/week of non-vigorous physical activity) or vigorously activity (>1600 kcal/week of physical activity that included vigorous physical activity). Selection bias is a potential problem when assessing physical activity behaviors in college students enrolled in an activity based class as it is possible a more health- conscious, physically active student is more likely to enroll in such a class. As a result, their physical activity levels may be different from the general population of college students. However, no previous study has addressed directly whether this selection bias exists. Our initial results show that this selection bias exists as students enrolled in the healthy lifestyles class were more active than students who were not enrolled in this class. However, the large percentage of kinesiology majors, who are more active than non-kinesiology majors, were enrolled in the healthy lifestyles class and accounted for the higher physical activity levels among students enrolled in the healthy lifestyles class. To illustrate this, when stratifying the sample by class, kinesiology majors enrolled in the healthy lifestyles class were 1.8 times more likely than non-kinesiology majors enrolled in the healthy lifestyles class to be in the second quartile, 2.3 times more likely to be in the third quartile, and 4.1 times more likely to be in the highest quartile. Furthermore, when comparing the non-kinesiology majors enrolled in the healthy lifestyles class to 104 those enrolled in the communications class, there were no differences in physical activity participation. These results suggest that when assessing physical activity behaviors in college students enrolled in an activity based class, it is important to address the potential selection bias that may exist due to a large percentage of kinesiology majors enrolled in that class. Our results also suggest that the physical activity selection bias does not exist among non-kinesiology majors as more physically active non-kinesiology majors were not more likely to enroll in an activity class. Therefore, after accounting for students who are kinesiology majors, our results suggest that physical activity participation among students enrolled in a healthy lifestyles class are generalizable to the entire college student population. Physical activity may decline throughout enrollment in college due to the increasing demands of course work, pressures of gaining independence, stress related to entering the workforce, or other responsibilities that inhibit time spent participating in physical activity. Although, the age related decline in physical activity from adolescence into young adulthood is well documented 9’ '5’ 29’ 46’ 68 there are few studies which have assessed this decline during enrollment in college. Our results show that older students were less likely to be physically active. Leslie and colleagues 4' assessed age-related differences in physical activity participation in nearly 2500 college students. The authors reported that physical activity declined in males from 75% to 70% to 58% in 18-19 years olds, 20-24 year olds, and 25-29 year olds, respectively. Females declined from 66% to 56% to 46% in the same age groups. Our results illustrate a similar decline as 32% of 18 years olds were in the highest physical activity quartile compared to 24% of 19 year olds, 23% of 20 years olds, 23% of 21 year olds, and 18% of 22 year olds. Both our data and 105 those of Leslie et al 4' are limited to cross-sectional comparisons and as a result we were unable to assess the longitudinal declines in physical activity during college. Future studies Should focus on assessing physical activity behaviors during undergraduate enrollment to determine if and when the added demands and pressures that occur during college contribute to the decline in physical activity. In the United States, there are substantial racial disparities in health across a lifespan and physical inactivity may be a contributing factor as African Americans are less active than Caucasians 30. There is limited research regarding racial/ethnic differences in physical activity within a college population. In order to contribute to the limited body of literature in African American college students, Ford and Goode 23 assessed daily physical activity levels in 224 students enrolled in an urban African American university. The authors reported that only 45% participated in daily physical activity of which 60% were males. The authors were unable to assess racial disparities because the sample was entirely Afiican American. Harris and colleagues 29 assessed longitudinal differences in physical activity participation between Afiican American and Caucasians from adolescence into young adulthood. In contrast to our results which show that African Americans are less active, there appeared to be little difference in physical inactivity between African Americans and Caucasians. A potential reason for the contrasting results is that our study used a more detailed assessment of physical activity. In addition, our study focused specifically on college students while Harris et a1 29 did not limit their study sample to college students. Due to the limited number of study participants who considered themselves Hispanic (n=16), Asian (n=3 7), Pacific 106 Islander (n=2), or American Indian (n=l) we were unable to compare physical activity participation between these groups. In summary, our study found that males are more likely than females and Caucasians are more likely than African Americans to be physically active. In addition, there appears to be a decline in physical activity in college students with age. Initial results illustrated that students enrolled in a healthy lifestyles class were more active than students enrolled in a general communications class. However, the majority of this relationship was due to large number of kinesiology majors enrolled in the activity class. That is, non-kinesiology majors enrolled in the activity class had similar levels of physical activity compared to those not enrolled in the class. Furthermore, non- kinesiology majors enrolled in the activity class had lower levels of physical activity compared to kinesiology majors enrolled in the class. Although, physical inactivity is a major concern on college campuses, there is a lack of literature that describes who is more likely to be inactive in this population. Our results add to this limited body of knowledge and enhance our understanding of the determinants of physical activity participation in the college student population. 5.2 Effect of High School Sports and Other Leisure Time Physical Activities on Physical Activity in College The results of our study Show a positive dose response relationship between number of high school sponsored sports played and physical activity participation in college. In addition, regardless of high school sponsored sport participation, students who were either moderately or vigorously active in leisure time physical activities other 107 than school sponsored clubs or sports during high school were more likely to be physically active in college. The transition from high school into college is an event which may contribute to the decline in physical activity. Bray and Born ” compared vigorous physical activity levels between high school and the first year of college and showed that frequency of vigorous physical activity declined from an average of 3.3 sessions per week during high school to 2.7 sessions per week during college. Leighton and Swerissen 40 reported that 42% of college students thought they were less active compared to their last year of high school. The goal of this study was not to determine the level of decline in physical activity during the transition from high school to college, but to add to the current body of literature by assessing the influence of physical activity and sport participation during high school on physical activity behaviors during college. Our results show that participation inhigh school sponsored sports influences physical activity participation during college. Compared to the lowest physical activity quartile, college students who participated in a greater nrunber of high school sports were more likely to be physically active in college. Everhart and colleagues 2‘ examined the impact of participation in high school athletics on physical activity levels in 201 college students. They concluded that physical activity participation during college was not different between former high school athletes and non-athletes. In comparison to the previous study which dichotomized high school sport participation (yes/no), an advantage to our study is athletes were categorized based by the number of high school sports played. This offered a more detailed assessment of high school sport participation 108 allowing us to uncover the positive dose response relationship between high school sport participation and college physical activity participation. It is also important to assess the impact of high school physical activity participation outside of school sponsored sports or club sports as it may also have an impact on physical activity during college. Previous research has shown that physical activity participation tracks moderately (r=.57) from high school into college '0. However, previous studies have not distinguished between high school sport and leisure time physical activity. Our results also show that regardless of participation in high school sports, there is a positive dose response relationship between high school LTPA and physical activity during college. These results imply that any physical activity participation, even outside of high school sponsored sports, has a beneficial effect on physical activity levels during college. This finding supports the value of encouraging the promotion of leisure time physical activity to high school students via avenues such as school wellness programs and physical education classes. While the results of our study offer new insights on the relationship between physical activity participation in high school and college, there are limitations to this study. It is possible that sport and physical activity participation during a student’s senior year of high school may have a more direct impact on physical activity participation during college. However, it was not possible to assess physical activity participation during a student’s last year of high school as our survey focused on estimating high school physical activity and sport participation across all four years of high school. We also did not assess attitudes towards and barriers to physical activity as previous research has shown they are potential determinants of physical activity during the transition from 109 high school to college '0’ H . F urtherrnore, when assessing high school leisure time physical activities, we did not specifically assess participation in physical education classes, after school wellness programs, or other specific modes of physical activity promotion. Therefore, we were unable to account for the direct influence of these factors on college physical activity participation. In conclusion, our results suggest that there is a dose response relationship between participation in high school Sports and physical activity participation in college. Furthermore, our results suggest that regardless of high school sport participation, college students who are more active in non-sponsored high school activities are more active during college. This finding illustrates the importance of promoting both high school sport and physical activity participation to high school students. 5.3 Efl'ect of Enrollment in a Healthy Lifestyles Class on Change in Physical Activity The purpose of this aim was to assess the impact of enrolhnent in a healthy lifestyles class, designed to promote and educate college students about the importance of physical activity and other health related behaviors on physical activity participation. Each healthy lifestyles class was held twice per week and included a 50 minute lecture on a health related topic followed by a 50 minute planned activity designed to promote the participation in physical activity. A communications class was used as a comparison group as the students enrolled were not receiving the same education about physical activity. The results of our study showed that during a 15-week semester, students enrolled in the healthy lifestyles class were more likely to increase physical activity compared to students enrolled in the communications class. 110 The majority of research assessing physical activity participation in college students enrolled in activity based classes are cross-sectional and Show that 23-58% of those students are physically active 6’ 12’ 23‘ 5 1’ 56. Our baseline results showed that 63% of our population was classified as vigorously activity and 15% as moderately active which is slightly higher than previous reports. However, due to the different methodologies used to assess physical activity, it is difficult to make a direct comparison of our results with the previous literature. Furthermore, the change in physical activity during enrolhnent in the class could not be assessed as the previous studies did not include a follow-up assessment of physical activity. Our study, which included the assessment of physical activity at the beginning and end of enrolhnent in the two classes provides insight on the impact of enrollment in a healthy lifestyles class designed to promote physical activity participation compared to the communications class. Our results showed that 23% of students enrolled in the healthy lifestyles class increased physical activity during the semester compared to only 14% of those enrolled in the communications class. In addition, 36% of communications students decreased physical activity during the semester compared to 31% of healthy lifestyles students. Furthermore, changes in physical activity as a result of the healthy lifestyles class were similar for kinesiology majors and non-kinesiology majors. The effectiveness of classroom-based physical activity interventions in college students is uncertain. To the best of our knowledge, results fiom only two previous intervention studies have been published in college students 42’ 6’. Project GRAD was developed to promote the adoption and maintenance of physical activity among young adults transitioning from the university into adult roles. Three hundred and thirty-eight 111 college seniors from a large urban university in the United States were randomized into either a class designed to promote physical activity or a control class which covered general health topics. Each subject completed a 7-day physical activity recall at the beginning and end of the 15-week classroom based intervention. Results showed the intervention had no Significant effects on total leisure time physical activity participation in men. However, in women, the intervention improved total leisure time physical activity and increased participation in flexibility and strengthening exercises 6'. Leslie and colleagues 42 assessed the impact of a classroom based intervention at two urban university campuses. A physical activity program was implemented at one campus over an 8 week period and included offering students fitness assessments and a free activity class of their choice. Physical activity was assessed via a self-reported questionnaire given at the beginning and end of the 8-week period. The authors reported that students who received the intervention were more vigorously active 42. The results of our study contribute to the limited research on classroom based interventions in college students as we found students enrolled in the activity based class were more likely to increase physical activity participation than those enrolled in a non-activity based class. In contrast to Project GRAD 6' which included only college seniors (i.e., older students), our study included mostly freshmen and sophomores (roughly 50% of our sample were 19 years or younger). Furthermore, Leslie et al 42 limited their intervention period to 8 weeks, whereas, our study spanned the entire duration of a 15-week semester. Limitations to our study include the duration of the study was only 15 weeks. In addition, only two time-points were used to assess the change in physical activity over the semester. This study was observational as we were unable to randomize students into 112 the healthy lifestyles and communications classes. In addition, we did not assess variables such as attitudes towards physical activity and readiness to begin a physical activity program which could partially explain why some students enrolled in the healthy lifestyles class were more likely to increase physical activity participation. In conclusion, results of our study show that both male and female students enrolled in an activity based class are more likely to increase physical activity participation during a 15-week semester. Although these results illustrate that an activity based class may serve as an opportunity to promote and increase physical activity participation in college students, there is minimal research confirming these findings. Therefore, our study provides valuable insight into the short term impact of enrollment in a healthy lifestyles class. 5.4 Assessment of the Effect of Using Different Specifications of Physical Activity Energy Expenditure Physical activity assessment is dependent on frequency, intensity, and duration of the activity. However, the current literature that assesses physical activity participation in college students lacks consistency in the use of these factors 36. In order to help address this issue, an underlying aim of this study was to determine if results changed when different specifications of physical activity were used. Therefore, physical activity participation was assessed as a continuous variable (kcal/kg/wk), an ordinal variable (quartiles of kcal/kg/wk), and a categorical variable (meeting the national recommendations for physical activity). Final multivariate results were similar regardless of which outcome was used. Each outcome indicated that males, kinesiology majors, 18 113 year olds, Caucasians, and students who were active in high school were more likely to be physically active in college. The majority of previous studies examining physical activity in college students used the criteria of meeting the national recommendations for physical activity I" 26’ 44' 48‘ 57. For example, meeting the recommendations for vigorous physical activity is considered engaging in physical activity that makes an individual sweat or breath hard on three or more days per week for 20 minutes per occasion. Although this variable is derived using a combination of frequency, intensity, and duration of physical activity, the problem within this approach is the inability to differentiate between those who participate in substantial amounts of vigorous physical activity and those who barely meet these recommendations. In order to assess the entire spectrum of physical activity, a continuous physical activity variable (kcal/kg/wk) can be calculated using a combination of frequency, intensity, and duration of physical activity. A limitation associated with measuring physical activity as a continuous variable is that the variable is unlikely to be normally distributed. As shown in the results of this study (Figure 4), our data are positively skewed due to the disproportionate number of study participants at the lower end of the distribution. To successfully normalize the data, a log transformation was needed. However, transforming the data increased the difficulty of interpreting the results as physical activity participation was reported in terms of log kcal/kg/wk. After carefirl analysis, we concluded the ordinal physical activity variable was the most favorable variable to use for our study. Quartiles of physical activity were derived from the continuous outcome variable which allowed for the assessment of levels of physical activity participation without the need for transforming the data. 114 The ordinal outcome variable allowed us to use ordinal logistic regression for data analysis. Ordinal logistic regression is a preferred method of regression analysis with ranked data because it calculates a single odds ratio that describes the magnitude of the relationship across different dichotomizations of the data (e.g. quartile 1 compared to quartiles 2-4 combined, quartile 1-2 compared to quartile 3—4, and quartile 1-3 compared to quartile 4) 47. This allows for less cumbersome interpretation. However, the underlying proportional odds assumption of ordinal logistic regression assumes the odds ratio is consistent across each dichotomization of the ordinal data. If this assumption is not met, the single odds ratio that is used to describe the data can not be used to adequately describe the magnitude of the association. As a result, a different regression technique such as multinomial logistic regression is needed. In our study, gender, race, and BMI did not meet the proportional odds assumption and as a result the odds ratios derived from the ordinal logistic regression models were not appropriate. Therefore, we used a multinomial regression in place of the ordinal logistic regression. A multinonrial logistic regression is used for categorical data that is not ranked. As a result, it does not require the proportional odds assumption. A multinorrrial regression determines the odds ratios for each category compared to a designated referent category. In our study the referent category was the lowest quartile of physical activity. The limitation to this method is that it produces more odds ratios than the ordinal regression. However, upon closer examination of our data, we found that the multinomial logistic regression provided a more detailed assessment of the data that the ordinal regression would have missed. For example, the bivariate unadjusted odds ratios generated by ordinal logistic regression indicated that college students participating in 115 four or more high school sports were 2.7 times more likely to be in a higher physical activity quartile. When analyzing the same data using multinomial logistic regression a dose response relationship was uncovered that was missed by ordinal regression. That is, when compared to the lowest physical activity quartile during college, students who participated in 4 or more high school sports were 2 times more likely than those who played zero high school sports to be in the second quartile, 2.1 times more likely to be in the third quartile and 4.5 times more likely to be in the highest quartile. Thus, the multinomial model showed a range of odds ratios between 2.0 and 4.5, whereas the proportional odds were 2.7. In conclusion, the use of a categorical, ordinal, or continuous outcome variables provide similar results when describing physical activity participation in college students. However, researchers should consider the potential limitations when using these variables. Using a categorical outcome variable that assesses meeting the recommendations for physical activity fails to distinguish between college students who participate in high amounts of vigorous physical activity and those who do not. When using the continuous variable, it is a possibility that a large number of college students will be on the lower end of physical activity participation causing a non-normal distribution. Although our results showed that a log transformation successfully normalized that data, this complicates the interpretation of the outcome variable. Therefore, we suggest the use of an ordinal variable to describe physical activity participation in college students due to its ease of interpretability and ability to assess levels of physical activity participation. 116 5.5 Assessment of Supplemental Analysis Results on the Impact of Enrollment in a Healthy Lifestyles Class A series of supplemental analyses were conducted to address the limitations of the modeling strategies used in the primary analysis of this project. The goal of the supplemental analyses was to offer additional insights into our primary results and attempt to provide alternate interpretations of the impact of enrollment in the healthy lifestyles class on physical activity. One limitation of the primary modeling strategies was loss-to-follow-up during the 15-week study period as these strategies assess data only from those who completed both the baseline and follow-up surveys. To address this limitation, a modeling strategy was used that included values for all study participants regardless of participation in the follow-up survey. Results of this supplementary analysis differed from our original analysis as it revealed that enrollment in the healthy lifestyles class did not influence physical activity participation over the 15-week study period. This supplemental analysis was conducted on the level of the population and assessed the impact of the healthy lifestyles class on students enrolled in the class as a whole. A possible way to offer a more detailed assessment of the impact of the healthy lifestyles class was to assess if any differences existed when using a subject-specific based modeling strategy. That is, to determine if the effect of the healthy lifestyles class different when considering the level of the individual as compared to the population. Although there is a trend that enrolhnent in the healthy lifestyles class improves physical activity at the individual level, the p- value (p = .102) was not significant. These results suggest the positive impact of the healthy lifestyles class on physical activity shown in our original analyses may in part be 117 due to baseline differences between the two classes that cannot be entirely controlled by adjusting for a variety of covariates. A second modeling strategy used as an alternative to the change score approach as its calculated value may be misleading. The results of this analysis, which used physical activity at the end of the semester as the outcome variable regressed upon the exposure variables which included physical activity at baseline, showed that healthy lifestyles students were more physically active at the end of the semester. However, similar to our original analyses, the limitation of this modeling strategy was that it did not account for the loss-to-follow-up as only 765 subjects were analyzed. Each semester there are six sections of healthy lifestyles classes which are taught by different instructors. Each section includes approximately 50 of the nearly 300 students enrolled in the healthy lifestyles class per semester. Because of the nested structure of this class, it was necessary to assess if any differences in physical activity existed between the different sections. As expected, physical activity was not altered by the section of a healthy lifestyles class. It was also important to assess whether physical activity during the 15-week study period differed between students enrolled in either the fall or spring healthy lifestyles class. Our results showed that when compared to the students enrolled in the communications class, students enrolled in the healthy lifestyles class during the spring semester were more likely to improve physical activity whereas, students enrolled in the healthy lifestyles class during the fall were not. These results suggest one of two possibilities. First, there are unique characteristics among students enrolled in the spring healthy lifestyles class which makes them more likely to improve physical activity as a 118 result of the class. The only difference we found at baseline between the fall and spring healthy lifestyles classes was a higher percentage of non-kinesiology majors were enrolled during the spring semester. This difference may imply that more non- kinesiology majors enroll into the spring healthy lifestyles class with the goal of making improvements in physical activity. Second, there is a seasonal effect on physical activity as students are more likely to become more active during the spring semester when compared to the fall semester. A limitation is that we do not have data from a spring communications class in which we could specifically assess differences in physical activity between and within the different semesters. In addition, we do not have data assessing barriers to physical activity that may change during a semester or the readiness students to change physical activity during the semester. Our analysis also showed that males were more likely to decrease physical activity during the semester than females. Previous research in college students has Shown that females are more likely to improve physical activity as a results of a physical activity interventions 6'. Therefore, it is possible that females are more likely than males to improve physical activity as a result of enrolhnent in the healthy lifestyles class. However, an additional supplemental analysis showed that impact of enrollment in the healthy lifestyles was Similar within gender. Therefore, it is likely that males decrease physical activity because they are more active than females at the beginning of the semester. In conclusion, the supplemental analyses preformed provide additional insight into the initial results of this study. These results contradicted our original findings in that physical activity does not appear to be influenced by enrolhnent in a healthy 119 lifestyles class. In addition, a potential seasonal effect on physical activity was revealed as students enrolled in the spring healthy lifestyles class were more likely to improve physical activity during a lS-week semester. 5.6 Future Studies of Physical Activity in College Students The third aim of this study was to develop a rationale/methodology for a future study assessing physical activity participation in college students. As stated throughout the literature review and discussion of this study, there is a lack of research assessing physical activity participation in college students. The majority of physical activity research in college students is cross-sectional 6’ ”’ '2’ 28’ 43’ 58. A recent meta-analysis indicated that research on college student physical activity is limited by: 1) the lack of studies in this population, and 2) inconsistent methodology used to assess physical activity 36. After the comprehensive literature review performed in this dissertation and the information gained from the results of this study, we concluded there is a need to assess physical activity and health behaviors of college students across and beyond the four years of enrollment in a university. Our findings provide interesting insight on the future direction of physical activity studies in college students. We were able to uncover important determinants of physical activity in college students such as gender, age, race, and major. Although this information contributes greatly to the limited body of knowledge regarding physical activity in college students, it does not address questions such as “Why are males more active than females?”, or “Why are older college students less active?”. As a result, future research should attempt to answer these questions by assessing attitudes towards 120 physical activity, readiness to participate in physical activity, self efficacy of physical activity, and barriers to physical activity. It is also important for future studies to assess physical activity and health behaviors throughout and beyond enrollment at a university. First, our results illustrate that enrollment in a healthy lifestyles class may benefit physical activity participation over a 15-week period. To determine if there is long-terrn impact of enrolhnent in the healthy lifestyles class, there is a need to follow these students beyond 15-weeks and throughout enrollment in the university. Second, our results suggest an age related decline in physical activity during enrolhnent in college. By assessing college students longitudinally throughout four years of university enrolhnent, it is possible to uncover the determinants for this age related decline. In addition, it would be possible to determine if and when there is a critical time period in which physical and health behaviors decline. Third, future studies should also focus on following college students beyond enrollment at a university and into adulthood. By following these students into adulthood, researchers could determine if changes in health behaviors during college affect lifelong physical activity participation. Future studies Should include a detailed assessment of physical activity similar to what was used in this study. Specific questions should be asked about intramural sport participation during college, club and/or varsity Sport participation, and enrollment in activity/sport based classes to determine their impact on physical activity participation during college. A detailed assessment of physical activity in combination with questions aimed at determining why there are differences in physical activity among college students will add to the existing physical activity literature in this population. The 121 methodology of a study designed to expand the results of this study and further address the limitations of the current body of literature addressing physical activity participation in college students is shown in Appendix F. 5.7 Conclusion AS stated by Healthy Campus 2010, physical inactivity is a major concern among college students 5. Despite this concern, there is a lack of physical activity data in this population 36. Therefore, the results of this study add significantly to the body of literature as we have shown various determinants of physical activity in college students and the positive impact of a healthy lifestyles class on physical activity participation. In addition, our study has also provided the foundation for the methodology needed in future studies. 122 Appendix A Consent Form Dear Student: Researchers from Michigan State University would like you to answer some questions about your physical activity and health behaviors through an intemet survey. To protect your privacy, all information you provide will remain confidential. You will have one-week from the in class presentation to complete the baseline survey. Please take you time and fill out the survey completely. It Should take approximately 10 minutes. At the end of the semester and throughout the following years, you will be informed via e-mail when to take a follow-up survey using the same ID and password that you created. Remember, this survey is confidential. Your privacy will be protected to the maximum extent allowable by law. Participation is voluntary, you may choose to not participate at all, or you may refuse to answer certain questions or discontinue participation at any time. There are no known risks associated with participation in this study. You will not benefit from participation in this study. However, your participation in this study may contribute to the understanding of physical activity and other health related behaviors of college students. A ticket number has been placed at the bottom of this page. This number will allow you to enter the survey and create a userlD and password. These following steps will provide you access to the survey. 1) First locate the 5-digit ticket number on the bottom of this page 2) Enter the website http://lsecommtechlab.msu.edu/ 3) Under “New Users”, enter your five digit ticket number into the box on the webpage then click “submit”. 4) Choose a userID and password for this survey then click “continue”. PLEASE REMEMBER THE USERID AND PASSWORD. YOU WILL NEED THIS FOR THE FOLLOW-UP SURVEY AT THE END OF THE SEMESTER. 5) Fill out the information page (i.e., What are the first two letters of your mother’s first name?) and click “save”. This information will be used if you forget your userlD or password. 6) Complete the survey. 7) Close the webpage when the survey indicates you are done. Upon completion of the survey, please retain the ticket number that you created. Take your ticket number to Joann lanes in Room 3, 1M Circle in which you will be redeemed with a coupon for a the ice cream cone at the Dairy Store. If you have any questions about the study, please contact me (Joshua Ode) or James Pivamik (address: 3 1M Circle, East Lansing, MI 48824, phone: 353-3 520, e-mail: jimpiv@msu.edu). If you have questions or concerns about your rights as a research participant, please feel free to contact Peter Vasilenko, Ph.D., Director of the Human Subject Protection Programs as Michigan State University: (517) 355-2180, fax: (571)432-4503, e-mail: irb@msu.edu, or regular mail: 202 Olds Hall, East Lansing, MI 48824. Thank you for your participation. Ticket Number Joshua Ode Doctoral Candidate: Department of Kinesiology Phone: 355—4734 e-mail: odeioshu@msu.edu 123 Appendix B Questionniare 1. What is your age? 17 18 19 20 21 22 23 24 25 or greater r'e‘qv runes 9‘!” 2. What is your gender? a. Male b. Female 3. How do you describe yourself? a. American Indian or Alaskan Native b. Asian c. Black or African American d. Hispanic or Latino e. Native Hawaiian or Pacific Islander f. White g. Other 4. What best describes your current living status a. On-campus b. Off-campus apartment/house with friends c. Off-campus with parents/guardians d. Off-campus alone e. Other 5. Are you a Kinesiology Major? a. Yes b. No 6. During the past month, did you participate in any physical activities or exercises such as running, calisthenics, golf, gardening, walking, etc.? a. Yes b. No If yes, please continue to question #2 If no, please continue to question #13 7. Using the list provided, click on the physical activity or exercise you currently participate in most often? 124 8. 10. ll. l2. 14. 15. How many times per week do you usually participate in this activity Less than 1 stress-99's» \105Ut-hWN—t Using 15 minute increments, how long do you usually participate in this activity? 15 30 45 60 75 90 greater than 90 (Present-rs» Do you regularly participate in a second physical activity or exercise other than what was previously mentioned? a. Yes b. No If yes, please continue to question #6 If no, please continue to question #13 Using the list provided, click on the physical activity or exercise you currently participate in the second most often? How many times per week do you usually participate in this activity a. Less than 1 b. l c. 2 d. 3 e. 4 f. S g. 6 h. 7 . Using 15 minute increments (15, 30, 45, 60, 75, etc.), how long do you usually participate in this activity? Do you regularly participate in a third physical activity or exercise other than what previously mentioned? a. Yes b. No If yes, please continue to question #10 If no, please continue to question #13 Using the list provided, click on the physical activity or exercise you currently participate in the third most often? 125 16. How many times per week do you usually participate in this activity 17. 18. 19. 20. 21. Less than 1 F'QPWPP-PP’P mama-wro— Using 15 minute increments (15, 30, 45, 60, 75, etc.), how long do you usually participate in this activity? a. 15 b 30 c. 45 d. 60 e 75 f. 90 g greater than 90 On average, how many hours do you watch TV per day? Less than 1 1 2 3 4 5 or greater ”PP-.09"? On average, how many hours of video games do you play per day? Less than 1 1 2 3 4 5 or greater finesse On average, how many hours do you spend surfing the intemet, writing e-mails, or completing class work on a computer per day (do not include playing video games)? a. Less than 1 b. 1 c. 2 d. 3 e. 4 f. 5 or greater Did you participate in any sponsored and/or club sports or activities (i.e., basketball, cheerleading, marching band, etc.) during high school? a. Yes b. No If yes, please continue to question #14 If no, go to question # 126 22. Using the list provided, click on all of the sponsored or club sports/activities that you participated in. Baseball Basketball Cheerleading Cross Country Dance Field Hockey Football Golf Ice Hockey Marching Band Pom Pons Rugby Soccer Softball Swimming Track Volleyball other F-P'Peear—F‘r'r'a‘mrfieeop’e 16. How many times per week did you usually participate in these sports/activities? Less than 1 rr'r'e‘qcrneensre 0 17. When you took part in these activities, how many minutes did you usually participate? 30 60 90 120 150 sees?!» 18. During high school, did you participate in any non-school sponsored leisure-time physical activities or exercises such as running, sports, gardening, etc.? a. Yes b. No 19. Using the list provided, click on the leisure-time physical activities or exercises you participated in most often during high school (choose no more than 3 activities). 127 20. On average, how many times per week did you participate in these activities combined? a. 1 b. 2 c. 3 d. 4 e. 5 f. 6 g. 7 h. 8 i. 9 j. 10 or greater 21. When you took part in these activities combined, how many minutes did you usually participate? a. 15 b. 30 c. 45 d. 60 e. 75 f. 90 g. greater than 90 24. What is your height in inches? 25. What is your weight in pounds? 26. What section of KIN 121 are you currently enrolled? a. 001 b. 002 c. 003 d. 004 e. 005 f. 006 128 Appendix C Log Transformation Data for Kcal/kg/week Figure 8. Frequency distribution for the continuous physical activity outcome variable ZOO-j f— 150'1 >* _ 0 C G 3100 e 1 IL h—r 50- _~ 0 —l_ — — — l 0.00 200.00 400.00 kcalslkglwk Table 15. Mean, skewness, and kurtosis data for the continuous physical activity outcome variable Mean 34.17 Standard Deviation 33.81 Skewness 3.29 Standard Error of Skewness 0.08 Kurtosis 20.50 Standard Error of Kurtosis 0.16 129 Figure 9. Frequency distribution for the log transformed continuous physical activity outcome variable 100- 80- ‘ fl1.. r— p. m l— l— 6‘ so- 1: or 3 cr 2 u. 40- ‘ _ F — '1 20— 1 — n i--1 0 I H I ’ ’ r I I -1.00 0.00 1.00 2.00 3.00 4.00 5.00 6.00 Log of kca llkglweek Table 16. Mean, skewness, and kurtosis data for the log transformed continuous physical activity outcome variable Mean 3.19 Standard Deviation 0.94 Skewness -0.62 Standard Error of Skewness 0.08 Kurtosis 0.76 Standard Error of Kurtosis 0.17 130 Appendix D Results for Continuous Physical Activity Outcome Variable Table 17. Descriptive data for the log kcal/kg/wk Variable N mean (139 PA) SD (Log PA) fstatistic p-value Gender Male 314 3.51 0.87 60.562 0.000 Female 557 3.01 0.93 Class Healthy Lifestyles 435 3.33 0.90 19.356 0.000 Communications 436 3.05 0.96 Major Kinesiology 190 3.55 0.83 36.778 0.000 Other 677 3.09 0.95 Race African American 56 2.90 1.26 3.210 0.040 Other 69 3.22 0.91 Caucasian 746 3.22 0.91 Age 22 or older 92 3.11 0.96 0.893 0.110 21 134 3.11 0.96 20 180 3.21 0.91 19 247 3.13 0.98 18 218 3.33 0.90 Living Status On-campus 418 3.28 0.90 7.400 0.007 Off-campus 448 3.11 0.97 High school sports 4 or more 114 3.44 0.94 5.789 0.000 3 sports 218 3.30 0.90 2 sports 225 3.21 0.87 1 sport 177 3.07 0.90 0 sports 137 2.95 1.10 High school LTPA vigorous 277 3.50 0.87 23.880 0.000 moderate 75 3.52 0.97 inactive 265 3.00 0.85 sedentary 253 2.96 0.99 TV 4 or more hours 66 3.07 1.03 2.190 0.088 3 hours 111 3.08 1.00 2 hours 227 3.13 0.99 less than 2 hours 467 3.27 0.88 VG 0 hours 615 3.09 0.97 13.800 0.000 1 hour or less 202 3.48 0.80 2 or more hours 54 3.26 0.91 BMI Obese 44 3.24 0.92 0.918 0.400 Ovenrveight 171 3.27 0.98 Normal weight 637 3.16 0.93 131 Table 18. Multiple regression analysis results for the log kcal/kg/wk R Std. R square Variable 8 Error P-value R square chagqe P-value High school LTPA -0.166 0.025 0.000 0.256 0.066 0.066 0.000 Gender -0.463 0.062 0.000 0.333 0.111 0.048 0.000 Major 03% 0.072 0.000 0.383 0.147 0.036 0.000 High school sports -0.104 0.023 0.000 0.408 0.167 0.200 0.000 Television viewing 0.105 0.031 0.001 0.422 0.178 0.011 0.001 132 Appendix E Results for Categorical Physical Activity Outcome Variable Table 19: Descriptive data for meeting the recommendations for physical activity Chi Inactive Moderate Vigorous square Variable N N % N % N % p-value Gender Male 321 48 15% 28 9% 245 76% 0.000 Female 587 143 25% 109 19% 335 57% Class Healthy Lifestyles 453 73 16% 58 13% 322 71 % 0.000 Communications 455 118 26% 79 17% 258 57% Major Kinesiology 193 19 10% 22 1 1% 152 79% 0.000 Other 71 1 172 24% 1 15 16% 424 60% Race African American 71 31 44% 14 20% 26 36% 0.000 Other 78 25 32% 10 13% 43 55% Caucasian 759 135 18% 113 15% 511 67% Age 22 or older 101 28 28% 18 18% 55 54% 0.256 21 142 37 26% 19 13% 86 61% 20 185 33 18% 26 14% 126 68% 19 255 54 21% 41 16% 160 63% 18 225 39 17% 33 15% 153 68% Living Status On-campus 437 81 18% 69 16% 287 66% 0.175 Off-campus 466 1 10 23% 68 15% 288 62% Television viewing 4 or more hours 71 21 30% 11 15% 39 55% 0.067 3 hours 118 32 27% 19 16% 67 57% 2 hours 236 52 22% 40 17% 144 61% less than 2 hours 483 86 18% 67 14% 330 68% Video game use 0 hours 640 137 21 % 108 17% 395 62% 0.003 1 hour or less 208 36 17% 18 9% 154 74% 2 or more hours 60 18 30% 11 18% 31 52% BMl Obese 50 16 32% 7 14% 27 54% 0.376 Overweight 180 37 24% 24 13% 1 19 66% Normal weight 657 134 20% 99 15% 424 65% High school sports 4 or more 118 17 14% 13 11% 88 75% 0.002 3 sports 222 37 17% 30 14% 155 70% 2 sports 234 43 18% 37 16% 154 66% 1 sport 186 51 27% 30 16% 105 57% 0 sports 148 43 29% 27 18% 78 53% High school LTPA vigorous 281 36 13% 19 7% 224 80% 0.000 moderate 75 12 16% 8 1 1% 56 74% inactive 276 58 21 % 62 23% 156 56% sedentary 276 85 31 % 48 17% 143 52% 133 Table 20. Unadjusted odds ratios for meeting the recommendations for physical activity OR OR moderate vigorous to p- to p- Variable inactive 95 % Cl value inactive 95 % Cl value Gender Male 0.77 0.45 1.30 0.321 2.18 1.51 3.14 0.001 Female 1.00 1.00 Class Healthy Lifestyles 1.19 0.76 1.86 0.453 2.02 1.44 2.82 0.001 Communications 1.00 1.00 Major Kinesiology 1.73 0.90 3.34 0.102 3.25 1.95 5.40 0.001 Other 1.00 1.00 Race African American 0.54 0.27 1.06 0.075 0.22 0.13 0.39 0.001 Other 0.48 0.22 1.04 0.062 0.45 0.27 0.77 0.003 Caucasian 1.00 1.00 Age 22 or older 0.76 0.36 1.61 0.474 0.50 0.28 0.89 0.018 21 0.61 0.29 1.25 0.175 0.59 0.35 1.00 0.049 20 0.93 0.47 1.86 0.840 0.97 0.58 1.64 0.919 19 0.90 0.48 1.66 0.730 0.76 0.47 1.21 0.240 18 1.00 1.00 Living Status On-campus 1.38 0.89 2.14 0.154 1.35 0.97 1.88 0.073 Off-campus 1.00 1.00 Television viewing 4 or more hours 0.67 0.30 1.49 0.329 0.48 0.27 0.865 0.014 3 hours 0.76 0.40 1.46 0.414 0.55 0.34 0.885 0.014 2 hours 0.99 0.59 1.66 0.962 0.72 0.49 1.072 0.106 less than 2 hours 1.00 1.00 Video game use 0 hours 1.29 0.58 2.85 0.528 1.67 0.91 3.089 0.099 1 hour or less 0.82 0.32 2.09 0.676 2.48 1.25 4.927 0.009 2 or more hours 1.00 1.00 BMI Obese 59% 0.23 1.49 0.267 0.53 0.28 1.02 0.057 Overweight 88% 0.49 1.56 0.658 1.02 0.67 1.54 0.939 Normal weight 1.00 1.00 High school sports 4 or more 1.22 0.51 2.90 0.656 2.85 1.51 5.41 0.001 3 sports 1.29 0.65 2.55 0.462 2.31 1.38 3.87 0.002 2 sports 1.37 0.71 2.63 0.343 1.97 1.19 3.26 0.008 1 sport 0.94 0.48 1.81 0.846 1.13 0.69 1.87 0.620 0 sports 1.00 1.00 High school LTPA vigorous 0.93 0.48 1.81 0.841 3.70 2.38 5.76 0.000 moderate 1.18 0.45 3.09 0.735 2.77 1.41 5.47 0.003 inactive 1.89 1.14 3.13 0.013 1.60 1.07 2.39 0.023 sedentary 1.00 1.00 134 Table 21. Adjusted odds ratios for meeting the recommendations for physical activity moderate vigorous to p- to p- Variable inactive 95 % Cl value inactive 95 % Cl value Gender Male 1.03 0.53 2.01 0.928 3.15 1.91 5.21 0.000 Female Major Kinesiology 1.70 0.85 3.40 0.900 3.08 1.78 5.33 0.000 Other Race African American 0.46 0.21 0.98 0.045 0.30 0.16 0.56 0.000 Other 0.50 0.22 1.16 0.107 0.55 0.30 1.02 0.057 Caucasian 1.00 1.00 Age 22 or older 0.93 0.41 2.14 0.872 0.63 0.32 1.22 0.172 21 0.68 0.32 1.45 0.318 0.74 0.42 1.31 0.305 20 1.05 0.51 2.14 0.900 0.92 0.52 1.62 0.769 19 0.98 0.52 1.85 0.944 0.72 0.43 1.19 0.198 18 1.00 1.00 High school sports 4 or more 1.09 0.44 2.75 0.848 2.50 1.22 5.11 0.012 3 sports 0.97 0.46 2.01 0.928 1.97 1.11 3.53 0.022 2 sports 1.17 0.58 2.35 0.664 2.04 1.16 3.58 0.013 1 sport 0.86 0.43 1.72 0.667 1.17 0.67 2.04 0.588 0 sports 1.00 1.00 High school LTPA vigorous 0.79 0.40 1.58 0.505 2.90 1.79 4.69 0.000 moderate 0.86 0.31 2.39 0.769 2.44 1.18 5.06 0.017 inactive 1.57 0.92 2.66 0.096 1.43 0.92 2.23 0.109 sedentary 1 .00 1.00 Video game use 0 hours 1.16 0.45 2.99 0.765 3.02 1.38 6.61 0.006 1 hour or less 0.73 0.26 2.00 0.537 2.33 1.05 5.13 0.037 2 or more hours 135 Appendix F Study Aim 3 Research Design Appendix E] Research Design In brief, the proposed research will begin with an undergraduate student population at a large Midwestern University. Assessment of these students will include an initial cross-sectional survey followed by a re-assessment prospectively at and interval of 15 weeks followed by intervals of 6 months until the study participant is not enrolled at the university. To achieve this projects specific aims, the analysis plan will include: 1) analysis of cross-sectional (baseline) data, as well as 2) analysis of the longitudinal data. Recruitment from a healthy lifestyles class student population and a communications class student population made it possible to compare these students cross-sectionally, after enrolhnent in their classes, and following enrollment in their classes. The first cohort will include students enrolled in a healthy lifestyles class and communications class recruited during the September 2007 and followed through December 2007. Additional follow-up surveys will be disseminated every March and October thereafter. The second cohort included students enrolled in these classes recruited in January 2008 and followed through April 2008. Additional follow-up surveys will be disseminated every October and March thereafter. Additional cohorts will be added in subsequent years. While these students will not be assigned at random to their classes, one of the focal points of this research will be to assess the initial and long-term impact of enrollment in a healthy lifestyles class (intervention group) compared to students enrolled in a communications class (control group). This research protocol will have three major elements: 1) completion of an anonymous online survey with standardized survey 136 questions and item sets and 2) a repetition of the online survey afier 15 weeks, 3) repetition of the online survey at intervals of 6 months. Figures 10 and 11 represent the timeline of this research protocol. Figure 10: Timeline for data collection for study participants entering the cohort in September Baseline Follow-up 2 Follow-up 4 F ollow-up 6 Follow-up 1 F ollow-up 3 Follow-up 5 Figure l 1: Timeline for data collection for study participants entering the cohort in January Baseline Follow-up 2 F ollow-up 4 Follow-up 6 F ollow-up 1 F ollow-up 3 Follow-up 5 137 Appendix F.2 Study Population The goal of this project is: l) to assess physical activity and health behaviors of college students during four years of enrollment at a university 2) to improve regular physical activity participation in college students with the expectation that this goal can be achieved, in part, via classroom based educational interactions that occur during the college years and 3) examine the long—term effects of a activity based class on physical activity participation. To give this project focus and to ensure a timely completion, undergraduate college students will be designated as the study population of interest. For reason of feasibility, undergraduates enrolled in the healthy lifestyles class (KIN 121) will be designated as the target population of primary interest because these students were receiving educational interactions intended to influence their physical activity levels. For a contrast with the experiences received during enrollment in the healthy lifestyles class, a second target population will be designated and will consist of undergraduate students enrolled in a communications class (COM 225). Students enrolled in this class will not receive the activity and health component of the healthy lifestyles class. An estimated 300 students will be enrolled in the healthy lifestyles class and 500 students enrolled in the communications class. Appendix F .3 Eligibility criteria (inclusion/exclusion) It was decided in advance that all enrolled students will be eligible for participation in this research. That is, there will be no inclusion or exclusion criteria applied to these undergraduate enrollees. Therefore, all 800 undergraduate students 138 enrolled in the healthy lifestyles or communications class will be eligible to participate in this research. Appendix F.4 Recruitment procedures Due to the anonymity of the data collection, recruitment was completed according to exempt approval status granted by the biomedical institutional review board at Michigan State University. In brief, the steps of the recruitment process were as followed. 9) Consent forms (Appendix A) will be distributed to all eligible study participants enrolled in study during the first week of class. 10) The consent form will include a survey admission ticket and instructions needed to access the on-line survey which will utilize the Longitudinal Surveillance Engine (LSE). 11) Verbal instructions will be given by a study investigator to ensure understanding of how to access the online survey. 12) Students assenting to participate will use their unique LSE admission tickets to complete the online assessment. 13) Study participants will be given 10 days to complete the baseline questionnaire. To encourage participation, a reminder e-mail will be sent to all study participants on the 5th, 7‘", and 9th days of the 10 day period. 14) A second series of reminder e-mails will be sent during the 15th week of the study period encouraging subjects to participate in the re-assessment survey. 139 15) Study participants will be given 10 days to complete the follow-up questionnaire. A reminder e-mail will be sent to all study participants on the 5th, 7‘”, and 9th days of the 10 day period. 16) Reminder e-mails will be sent every March and October encouraging subjects to participate in the long-term re-assessment surveys. 17) Upon completion of the baseline survey and follow-up surveys, students will be eligible to receive a coupon for free ice cream cone at the Dairy Store located at Michigan State University. Appendix F.5 Assessment Protocol: This research project will utilize the Longitudinal Survey Engine (LSE) which was recently developed at Michigan State University. The LSE is an intemet based survey engine that offers anonymity and privacy for exploring topics that might be too sensitive to effectively engage participants in scientifically meaningful disclosures. The LSE is designed for large sample longitudinal, research protocols. Under the LSE protocols, participants log in anonymously to create their own userID and passwords, and complete an initial baseline survey. Thereafter, participants are invited to return for follow-up surveys using the previously created userH) and passwords. The userID/password feature allows the investigator to monitor individual and group patterns of health and behaviors anonymously over time. The LSE can be menu-driven by authorized client-investigators using major intemet browsers (Internet Explorer, Netscape, etc.). The main features of the LSE used in the current investigation are described below: 140 g) h) i) k) 1) Each study participant will be provided with online access to the LSE (http://lse.commtechlab.msu.edu/login.php) The LSE software used a unique survey ID number to track each individual survey protocol (e. g., an initial online baseline questionnaire or assessment followed by longitudinal assessments), and also to track each participant's responses at baseline and over time. (The LSE built a unique ID number to track both the survey protocol and the individual participant. This ID number was called a 'coupon number.') Prior to completion of the initial baseline assessment, the designated participant will access the LSE website, key in his or her LSE coupon number, and will be prompted to create his or her own userID and his or her own password. This will be followed by prompting questions used to help the individual recall the userID and password if these were lost or forgotten (e.g., 'What is the first initial of your mother's maiden name (surname before marriage)?’ This approach will be used so designated participants can log in anonymously without direct or indirect linkage between the participant's identity and the survey responses. Thereafier, the web browser will display a study disclosure statement inviting participation. The participant will key the NEXT button to proceed to complete the survey, or can log out at any time. The LSE was designed to allow the participant to terminate participation and/or to skip over a survey item. Survey elements used in this investigation will include true/false, multiple choice, and short answer items. The LSE will keep track of successive log-ins by designated participants, which allows them to complete repeated measurements. Storage of the repeated measures 141 occurs within a database that encoded the userID and a date/time stamp. Number of logins and the interval between repeated measures are controlled by the investigator by Options given by the LSE. All responses are stored within a protocol-specific EXCEL spreadsheet on the LSE secure server. m) The LSE 'coupon management' system allows for the investigator to build an Excel database from a unique study-participant ID generated for each study participant. The database is imported to a word processing program for label-printing, which are delivered to the designated participants via a sealed envelope to ensure anonymity. Appendix F.7 Primary Outcome Variables College leisure time physical activity participation: At entry into the cohort, all study participants will be asked if s/he participated in any leisure-time physical activities or exercises such as running, calisthenics, golf, gardening, walking, etc. during the past month. If the study participant answers yes, a series of three remaining physical activity questions will be asked to assess the type, fi'equency (sessions per week), and the duration (average minutes per session) of activity. These questions are based on a similar previously validated instruments 1’ 3’ 33. A metabolic equivalent value (MET) will be assigned for each activity using the Compendium of physical activities to calculate weekly caloric expenditure 2. If the study participant reports participating in a second or third activity, the same series of questions will be repeated for each activity. The same series of questions will be repeated at the end of the 15-week testing period and throughout the follow-up surveys. These questions are similar to others used in physical activity survey research 45‘ 50. 142 Appendix F.8 Exposure Variables Gender: Gender will be defined as a dichotomous variable (male/female). Class membership: Class membership will be defined as a dichotomous variable (healthy lifestyles/commmlications). Age: Age will be defined as a categorical variable (18 years, 19 years, 20 years, 21 years, 22 or more years) Race: Race will be defined as a categorical variable (Caucasian, African American, Other (e.g. Hispanic, Asian, Pacific Islander, Native American)). Living Status: Current living status will be defined as a dichotomous variable (living on—campus, living off-campus). Major: Major will be defined as a dichotomous variable (kinesiology, other). Class standing: Current class standing will be measured as a categorical variable (freshman, sophomore, junior, senior). Body Mass Index: Data on self-reported height and weight will be collected and body mass index (kg/m2) will be calculated. Subjects with a BMI between 18.5 and 24.9 are considered normal weight, between 25 and 29.9 are overweight, and 30 or greater are considered obese. Sedentary Behaviors: Current sedentary behaviors will be assessed by determining the number of hours per day spent: 1) watching television, 2) playing video games, 3) using the computer for school related work, and 4) using the computer for non- school related work. High school sports: Participation in school sponsored sport activity will be assessed by asking each subject if s/he participated in any school sponsored and/or club 143 sports or activities (e.g. basketball, cheerleading, marching band, etc.) during the last year of high school. If the student participated in a school sponsored sport, s/he was first asked to identify each high school sport in which s/he participated. This was followed by asking: 1) how many months per year, 2) the average number of sessions per week, and 3) the average minutes per session s/he participated in these school-sponsored sports combined. Using MET values for each physical activity, average weekly caloric expenditure (kcal/kg/week) for a combination of all sponsored high school sports and non-school sponsored physical activity during high school was calculated. In this study, high school sport participation was defined as the number of high school sports played (0 sports, 1 sport, 2 sports, 3 sports, 4 or more sports). High school leisure time physical activity participation (high school LTPA): Participation high school leisure time physical activity will be assessed by asking if each subject participated in any non-school sponsored leisure-time physical activities or exercises (any activity that was not sponsored directly by the high school) such as running, sports, gardening, etc. during high school during the last year of high school. This was also followed by a series of questions asking: 1) how many months per year, 2) the average number of sessions per week and 3) the average minutes per session s/he participated in these non school-sponsored physical activities combined. Using MET values for each physical activity, average weekly caloric expenditure (kcal/kg/week) for a combination of all sponsored high school sports and non-school sponsored physical activity during high school was calculated. Using the responses from this series of questions, study participants were categorized as: l) sedentary (reported no non-school sponsored sport activity, 2) inactive (reported some leisure time activity but did not meet 144 the recommendations for moderate or vigorous activity), 3) meeting the recommendations for moderate physical activity, and 4) meeting the recommendations vigorous physical activity. High school sponsored sport activity was not assessed the same way as high school non-sponsored leisure time activity because the majority of study participants who played a high school sport (90%) met the recommendations for physical activity during high school sport participation. Grade point average (GPA): Current Grade point average will be assessed as a continuous variable. Physical activity readiness: Physical activity readiness will be assessed as a categorical variable (“I have never though about exercising”; “I have thought about exercising a few times, but can’t get started”; “1 exercise every now and then.”; I try to exercise regularly, but it’s hard to keep it up.”; “I am now exercising regularly.”) Physical activity barriers: Barriers to physical activity will be assessed as a categorical variable (School work; Lack of time; Weather; Motivation; Lack of facilities; Enjoyment of exercise; Other; Barrier do not prevent me fro exercise). Physical activity attitude: Attitude towards physical activity will be assessed on a likert scale (l=strongly agree, 5=strongly disagree). The following statements will be used to assess attitude towards physical activity: If I were to be physically active during my free time on most days it would help me cope with stress; If I were to be physically active during my free time on most days it would be fun.; If I were to be physically active during my free time on most days it would help me meet friends; If I were to be physically active during my free time on most days it would get or keep me in shape; If I were to be physically active during my fi'ee time on most days it would make me feel 145 better about myself; If I were to be physically active during my free time on most days it would give me more energy.; If I were to be physically active during my free time on most days it would make me better in sports, dance, or other activities; If I were to be physically active during my free time on most days it would make me hot and sweaty. Physical activity self eflicacy: Physical activity self efficacy will be assessed on a likert scale (l=strongly agree, 5=strongly disagree). The following statements will be used to assess physical activity self efficacy: I can be physically active during my free time on most days.; I can be physically active during my fiee time on most days even if I could watch TV or play video games instead; I can be physically active during my free time on most days even if it is very hot or cold outside; I can ask my best friend to be physically active with me during my free time on most days.; I can be physically active during my free time on most days even if I have to stay at home; I have the coordination I need to be physically active during my free time on most days; I can be physically active during my fi'ee time on most days not matter how busy my day is. 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