III I‘II I III I l I “I I II III I III .1.b_3 100 I .CDCDLC I A STUDY OF CERTAIN FACTORS INFLUENCING THE CULINARY QUALITY OF POTATOES. WITH SPECIAL REFERENCE TO THE COLOR OF BOILED POTATOES THESIS FOR THE DEGREE OF M. 3. Arthur Ward GIidden 1932 PLACE IN RETURN BOX to remove this checkout from your record. TO AVOID FINES return on or before date due. E DATE DUE DATE DUE DATE DUE 6/ IS Kz/Prol/Acc&Pres/CIRC/DateDueForms_20|5.Indd - pg] A STUDY OF CERTAIN ACTORS IITE'LUFEJCII-TG THE CULINARY gUALITY OF POTATOES, 11TH SPECIAL FETIRTEJCE TO THE COLOR OF BOILED POTMOBS THESIS AC BS'IOIF.’LEDGEIZEI T The author wishes to express his appreciation to Professor H. C. Meore and Dr. E. J. Miller for their guidance and helpful criticism given this work; to the manbers of the Experiment Station Chemistry staff who assisted in the scoring of the potatoes; and to all others who from time to time have given the author their COOpGration. 96413 A STUDY OF CELTAIII FACT RS I--i_ol CIZ'G I'IIFZ CULINARY U 001.011 OF BOILED POT ATOES OUTLILE I. . II‘ITRODUCTION II. PEVKITI 1‘ LITERATURE A. Factors Influencing the General Culinary Kalil]. ity o i‘ Pot a toes 3. Factors Relative to the Color of Cooked Potatoes III. nifl‘TI—‘IIIZ’I i A. General LIothodsof Procedure 1. Lletl od of Determining; ulinar y Quality 2. Method of Scoring 3. Liethod of Cooking; B. Part I. Factors Influencing the Ce enera l C‘O‘Ilir‘ar;r Quality of Potatoes 1. Proccc‘ture and Results a. Comparison of he Culin ary Cuality of Certain Varieties fro"; Various Sources b. The Influence of Maturity upon Culinary quality (a) Tests with mate rial i'I‘O’Z. IDate of Planting; Plo us (‘0) Tests t’i th material from Greenhouse Flots UALIT'I 0? P01". JOBS ‘IIITII .SE'ECIAL FER'IR'LTIICE '30 THE 10 ll (‘3 CO PO to Page c. Influence of Fertilizer Analysis and -ate of Application upon Culinvry Quality of irrigated and nor-irrigated plots 55 2. Discussion a. Influence of Varieties and their Source upon Culinary wuality 55 b. Influence of Katurity upon Culinary unality 36 C. Part II. Factors Influencing: the Color of Boiled Iotataes 37 1. Procedure and Results a. Effect of Poor Aeration of nature Russet Rurals upon Color 40 b. Effect of Door Aeration of I: ature Russet Rurals upon Color 40 0. Effect of Poor Aeration of 1952 Winter crop of Florida Spaulding Rose and Bliss Triumph potatoes upon Color QC 2. Discussion 47 IV. S 1.3 ARY AID CCII’JLUSIOIIS 49 A STUDY OF CERTAIN FACTORS IIIZELTIIICIIZTG TIE CULINARY QUALITY OF POTATOES, BITE-I SPECIAL R'EFIJRJEICE TO THE COLOR OF BOIIED POTATOES A Thesis Prepared by ARTHUR WARD {EDEN in partial fulfillment of the requirements for the Degree of Master of Science Department of Farm Crops IIZICHIGAN STATE COLLEGE OF AGRICULZURE AIID APPLIED SC 1932 -1'r' "t A'JA'CJIJ RTRODUCTION Uith the exception of the potatoes used for seed purposes, practically all of the potatoes produced in the United States are used.ibr human consumption. Consequently quality is an important factor in potato production and marke ting-5, . finality in potatoes is dependent upon two component factors, namely, market quality and culinary quality. Iarket quality of potatoes is determined by the general appearance of the tubers, attention being given to the size, type and unifornity of the potatoes as well as freedom from surface diseases and mechanical injuries. Culinary quality refers to the quality of the potatoes when prepared for table use. Culinary quality is determined by the cohesion of flesh or sluffing of the cortical layer, the color, tie texture and the flavor of the potatoes when cooked.' In recent years the culinary Quality of Lichigan potatoes has been severely criticized. This criticism is no doubt at least partially reSponsible for the range in price between Iichigan potatoes and potatoes produced in some other states. The price of Kichigan potatoes on the Detroit market during the first week of Decenber 1951 was 49 cents per bushel, compared with 31.10 per bushel for Idaho potatoes and 67 cents per bushel fer potatoes produced in Kaine. It is impossible to determine accurately the exact percentage of this difference which is due to poor quality, but it would be reasonable to conclude that the profitableness of the potato crOp in Michigan calld be greatly increased by improving the quality of the crop. The experiments reported in this paper are all more or.less of a preliminary nature. An attempt has been made to determine in what manner Midaigan potatoes differ in culinary quality from potatoes grown in Idaho, Kaine and Florida. A study of maturity'and certain cultural practices was also made in an attempt to ascertain the practices which are inducive to good culinary quality. Since the darkening after cooking is often a serious factor inhidiigan potatoes, a shady was made to determine the cause of this condition. REVIEW OF LITERATURE FACTORS INFLUENCING THE GENIRAL CULINARY QUALITY OF POTAZOES Gilmore (1905) working with the Doe's Pride variety found.that tubers produced on clay loam were poor in quality, being firm and soggy after boiling, while tubers produced on sandy loam were excellent in quality. The same investigator also reported that immature tubers were less mealy andxnore soggy than mature tubers. . Ashby (1906) woniing in England, summarized his investigations as follows: "Indications seem.to point to physical causes as exercising the greatest influence on quality, eSpeciaily temperature and water supply. Loamy soils give unifOrm warmth and water supply and,therefore, produce the best quality." East (1908) reported that to give good culinary quality the starCh content of the tuber should be high enough to fill the cells of the cooked tuber to the bursting point. Potatoes with a starch content above a certain point, approximately 18%, would easily fall to pieces, or if the cell walls were strong enough to hold together’under pressure they would necessarily be tough.and woody. Butler, Horrison and Boll (1913) concluded from their investigations that: 1. As the relative quality of the potatoes in- creased there was a decrease in the differentiation of the inner medulla. 2. The percentage of starch'had no direct influence upon the mealiness of the potato. 5. ifealiness is modified by tire water content, potatoes having a relatively high water content being less mealy than potataes of low water content. Headen (1927) reported that the results of 68 tests showed.that the application of nitrates produced potatoes of poor duality. Findlay (1928) made an investigation of the quality of potatoes in Scotland. A brief summary of his work is here given: 1. A better quality of potatoes was produced on sandy soil.than was produced on clay or peat soils. 2. Dry conditions favored good quality, whereas, wet conditions were apt to cause poor quality. 3. Regardless of wet or dry seasons early plantings gave extremely dry, mealy potatoes, while late plantings gave wet, soapy potatoes. 4. Excessive use of nitrogen and poorly balanced fertilizers resulted in poor quality. 5. The quality of the potatoes Was lowered by the omission of phosphorous from the fertilizer mixture. 6. The omission of potash caused many of the varieties to turn black after cooking. 7. Quality was impaired by line and the continued use of manure. 8. mosaic affected plants showed no difference in quality, but plants affected with leafroll tended to produce poor quality potatoes. Child and Williman (1929) feund that potatoes with a high percentage Of dry matter tend to be accanpanied by better texture and flavor than potatoes with a low percentage of dry matter. The work of heil.and Whittimore (1950) on.the relation of;mealiness in potatoes and the amount of potash in the fertilizer shows that boiled, baked and mashed potatoes are more mealy when fertilized with a high rather than a low quantity of potash. No correhation between starch content and mealiness was found. Peacock, Wright, Whitaman and Fuller (1930) made a study of the influence of storage temperatures upon the quality of potatoes. Their investigations showed that the cooking quality becomes better as the storage temperature increased from 32° F. to 60° F. The maximum culinary quality was found .in the tubers stored at 60° F. The tubers stored at the lower temperatures were poorest in quality due to the sweet flavor caused by the accumulation of sugars. 6. FACTORS RELATIVE T0 1 3 ODLOR OF COOKED POTATOES Several investigations have been made to determine the cause of the blackening of raw potatoes. Since there may be a relationship between this type of blackening and the blackening of potatoes after cooking it seems advisable to review some of the outstanding and more recent works. Reference will first be made to the oxidation enzymes of the potato in order to make the resume of these investigations more understand- able. Onslow (1931) classifies the potato as a plant giving oxygenase and tyrosinase reactions as well as peroxidase re- actions. Associated.with these enzymes are certain aromatic compounds with the ortho-dihydroxy grouping characteristic of catechol. Oxygenase (Onslow, 1951) catalyses the autoxidation of this group of aromatic substances. This reaction gives an organic peroxide, catechol peroxide, which is subsequently decomposed in the presence of water, giving hydrogen peroxide. The enzyme tyrosinase (Onslow, 1931) is closely associated with oxygenase, as no plant so far studied gives the tyrosinase reactions without also giving oxygenase reactions. Tyrosinase has the power to catalyze the oxidation of the amino acid, tyrosine, producing a pink coloration rhich gradually deepens to red.and finally to black. The black compounds thus formed are known as melanins. Peroxidase (Onslow, 1951) in the presence of hydrogen peroxide will oxidize a number of phenolic substances of different composition such as pyrogallol, catechol, x ‘ quinol, benzidine, guaiacum, guaiacol, p-cresol aid others. The nature of the actual oxidations associated pith peroxidase in the plant iS‘unkuown. uuen1m3mul.res iration is tdfihm; place in the iotats tuber the reactions of these three enzymes are held in a delicate balance and no bad effects are produced. Und:r conditions of abnormal resgiration certain of these enzymes catalyze reactions detrimental to the pot to tuber. Evidence of this is the blackening of raw potato tissue which may be b'oucht about by various causes. Khan a raw potato tuber is injured mechanically blackenin takes place. Cnslow (1931) reports this blackening as being typical of all plants containing oxygenase and a catechol compound. Th; discoloration is the result 01 tie oxidation of the aromatic substrate by oxy;2 enase and in addition the probagle secondary oxidation of other aromatic substances. Bartholomew (1913), Stewart and :ix (1917), Bartholomew and Bennett (1924) and others ‘1 ave shown that black heart develops when potatoes are stored where the oxygen supgly is not ample. According to Bartholomew (1913) an excess of oxygen accumulates in fine tubers when they are again placed in an ample atr losphere of oxygen. As a result, the en zylne tyrosina se catalyzes the oxidation of the amino acid tyrosine which has accumulated in a free Donn. As a final result, the tissue ultimately becomes black. While working with potato enzymes Onslow (1931) obs enved mmOpwpoH weaned: H0 @090 poqu: mama map Ho poaoo ozp com: Hapmsm cmcmxo pquonoQ w Ho poommm age .HHb canoe page: Mome oanpmeHmcoo menu 5 HH m detach... Mowam 0 HH m passe soaam seesaw sage o s H H mason 30H HP». 0 H. H Heston opfiea emflaoaamw ma 0 ma c Hosaou mafia mm 30H Hm». o o Hm Moose omom wfi 3:95 m on Page Mome wagon}, 3 Es mmHEHHmM H. HH m Hagen mpg 0 HH m . vases xvam meanmeHmsoo 0p meHHm 0» Ea gm .EoHHmH. H. b H Hwaao: 0» Es mmHaoHHmw o b H Hasnom mpfies nmflaoaamw ‘ ma 0 mm = Hasnoz . opfles :mHsoHHms o o aw Momma emssflne Am mmHHm m .02 4. . Hugo; M098 b HH m Hwanom 0» HHS. 0 HH m Has.H 8H #095 H. H. H HwfiHoH BEE ngOHHmN o b H HwSAoH 59% n “038.» 9393 m mH 0 mm : Hashes mpfl a o o aw sumac mmom wnfisaswmm e .02 mxowm :H HE. A: 93600 @2308 cough codmom .8832 93932 p04 me was as 533500 93.3 .HOHoo whoa whom 68.. .28 upmflg Homscfipqoov .HH> oHnma 7 . DI scuss I on The data presaited ir Tables V, VI and VII were obtained in three experiments unich were canducted to determine the influence of poor aeration in store e upon the color of boiled potatoes. The results of these ei,erimeits show that the black- ening after cooking was more frequent in the tubers which had been sealed in jars and later stored in cloth sacns. The length of the storage period in the sealed jars that was necessary to produce this condition varied with the different lots of potatqzs used. Ho relationship was apparent between the blackening of cooked potatoes and black heart. The changes tithin the potato tuber which are reSpo:sible fer the blackening after cooking are not definitely known. However, it is possible that these dianges are in some nanner associated with the reactions studied by Onslow, Bartholomew, etc., to which reference has previously been made. The results of the investigations of these experimentors help to form a basis for the following discussion of the blackenin: of potatoes after cooking. The normal respiration of the potato tuber is in- terfered with by poor aeration in storage. Under these conditions the amount of oxygen decreases very rapidly until it is either very small or entirely consumed. As the oxygen concentration decreases the carbon dioxide concentration increases. then the tubers are subsequently stored in a well ventilated place they 48. absorb oxygen rapidly. It is believed that associated Ydth this oxygen absorption there is an oxidation of some of the aromatic compounds present in the potato tuber. The fact diet potatoes which blacken after cooking appear normal before cooking is taken as evidence to indicate that this oxidation is inhibited before it is complete and that only intermediate products are fbrmed. Upon eXposure to air after cooking these intermediate products are auto-oxidized and the resultant black color is formed. It will be noted by a study of Tables V, VI and VII that the procedure followed did not produce blackening in certain lots after cooking. Koreover, certain inconsistencies occur in the lots which did blacken after cooking. These differences nay be due to inhibiting factors, or perhaps factors oflier than poor aeration have an influence upon the blackening of potatoes after cooking. The data su gest that maturity or the stage of the rest period or both may be such factors. Furthermore, the in- “’1 crease or carbon dioxide under conditions of poor aeration cannot be ignored, for it may also play a role in the blackening of potatoes. The data given in this paper are of’a preliminary nature. A more complete and thorough investiggati on is necessary before definite conclusions can be made concerning the cause of the blackening;of cooked potatoes. l. 5. 4. 49. UlIZARY :l‘TD COITCLUSIGZTS U) A comparison of the culinary quality of Kichigan New Standard Potato Club Russet Rurals, Iiichigan U. s. #1 Russet Rurals, Idaho Russet Burbanks, Kaine Green Hountains, and the 1952 winter crop of Florida Spaulding Rose and Bliss Triumph potatoes has been made. The results of this comparison indicate that the Kichigan New Standard Potato Club Russet Rurals and the Idaho Russet Burbanks were superior in culinary quality to the ifaine Green I-Eountains and the liichican U.S. 331 Russet Rurals. The Spaulding Rose and Bliss Triumph potatoes from Florida were intermediate in culinary quality, being superior to the Kaine Green Hountains and the Xichigan U. S. #1 Russet Rurals, but inferior to hichigan New Standard Russet Rurals and the Idaho Russet Burbanks. The results of an experiment to determine the influence of the date of planting of Russet Rural potatoes upon culinary quality indicate that early planting may improve the culinary quality. Cooking tests made on potatoes grown in the greenhouse indicate that a.heavy application of water for a few days previous to harvesting causes the potatoes to be so: y in texture nhen cooked. 5. 6. Experimental evidence was obtained mhich indicates that poor aeration in potato storafe may be one factor which causes potatoes to turn black When cooked. Bartholomew and other investigators produced bled: heart in similar experirents, but made no reference to the effect of poor aeration upon the color of the cooked potatoes. Black heart was pnbmiced in the'potatxas in the experiments which were stored under conditions of poor aeration. ho relationship was found, however, between black heart and the blackening of cooked potatoes. 51. LIERAIURE CITED Ashby, S. F. 1906 A contribution to the Study of Factors Affecting the duality and compostion of Potatoes. Jour. Agr. Sci. (England) 1:347-557. Bartholomew, E. T. 1913 Black heart of Potatoes. Phytopath 5:180-182. and Bennett, J. P. #1924_’ The Respiration of Potato Tubers in Relation of the Occurence of Black heart. Univ. Calif. Tech. Paper 14: 1-55. Butler, 0, Morrison, F. B., and Boll, F. E. 1913 Studies of the Factors Affecting the Culinary Quality of Potatoes. Am. Soc. of Aaron. 5:1-55. Bast, Edward W. 1908 A Study of the Factors Influencing the Improve- ment of the Potato. 111. Agr. Exp. Sta. Bul. 127: 575-456. Findlay, U. M. 1928 ,uality in Potatoes. Scot. Jour. Agr. 11:559-544. Gilmore, John V. 1905 ,uality in Potatoes. Cornell Univ. Agr. Exp. 3138. Bill. 250: 503-525. . Headden, William P. _ _ 1924 The Effects of Nitrates on the Composition of the Potato. Colo. Exp. Sta. Bul. 291:1-97. Neil, Bernice and Uhittemore, Kargaret 1930 The Relation Between Healiness in Potatoes and, the Amount of Potash.in tie Fertilizer. Am. Potato Jour. 7: 275-285. Onslow, Iuriel W. 1951 The Principles of Plant Biochemistry. Part I. Cambridge Univ. Press p. 123-172. Peacock, X. M., Wright, R. C., Uhiteman, T.E., and Fuller,Zlizabeth 1950 Differences in the Cooking Quality of Potatoes Due to Storage Temperatures. Proc. 17th Ann. fleeting of Potato Assoc. of Amer. 109:116. U1 PO Stewart, F. C., and Iix, A. J. 1917 Black heart and the Aeration of Potatoes in Storage. B. Y. Geneva Apr. Exp. Sta. Bul. 456: 521-562. Tinkler, Charles K. 1951 The Blackening of Potatoes after Cooking. biochem. Jour. (England) 25: 775-777. Manna use (mu. 993.124; '36 Dec 10 48 E 93.5.