EFFECT GF HORACE TEMPERAFURE AND ATMOSPHERE ON THE CHEPPEMG QUALEW 0F P'O'FATOES The“: for Hm Dog?“ of MpS. IEHCHEQAN STATE UNIVERSITY T‘ukaram Bhujangarao Chawan £965 ABSTRACT EFFECT OF STORAGE TEMPERATURE AND ATMOSPHERE ON THE CHIPPING QUALITY OF POTATOES by Tukaram Bhujangarao Chawan Modified levels of Carbon dioxide and oxygen in combination with controlled temperatures were studied to determine their usefulness to provide a reliable storage condition for potatoes to be used in the manufacture of potato chips. The project was of three years duration and the varieties used were: Kennebec, Arenac, Sebago, Emmet, Russet Rural and Russet Arenac. The temperatures used were from 34°F. to 50°F. The various gas level combinations of Carbon dioxide were from 5% to 10% and of oxygen from 1% to 5%. A chipping test, was carried out at regular inter- vals with potatoes, out of storage and after two, four and six weeks conditioning. The thickness of the slices, the frying fat, time and temperature were kept constant. A flavor evaluation technique was used. Tukaram Bhujangarao Chawan Kennebec and Arenac were found to store at 400F. and 5% C02, 5% 02 level, for 30 weeks and produce #5 color (highly acceptable) chips after two weeks conditioning. It was also found that at 34°F. with controlled atmosphere con- ditions and also in air, the potatoes could not be stored for more than 4-8 weeks in good chipping condition. It was observed that high carbon dioxide in combination with low oxygen levels in the atmosphere causes poor horticultural variety of the potatoes. It was found that the mold susceptibility varied with variety, temperature and controlled atmosphere storage conditions. Approved by ‘JMaj<:)Profe§§or EFFECT OF STORAGE TEMPERATURE AND ATMOSPHERE ON THE CHIPPING QUALITY OF POTATOES BY Tukaram Bhujangarao Chawan A THESIS Submitted to Michigan State University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Department of Food Science August 1965 ACKNOWLEDGMENTS The author wishes to express his appreciation to Professor I. J. Pflug, his Major Professor and chairman of the committee, under whose guidance this project was com- pleted and this manuscript prepared. The author also wishes to thank Dr. N. R. Thompson, of the CrOp Science Department, for his continuous support and help during the project. The author is very thankful to The Whirlpool Corpor- ation for their financial support of this study. A sincere thank you is extended to the author's wife Chandrajyothi, for her understanding and encouragement throughout the study. ii TABLE OF CONTENTS I. INTRODUCTION . II. REVIEW OF LITERATURE A. B C. D Potato growth and harvest Physical structure of the potato Chemical composition Utilization of potato crop l. Chipping industry 2. Frozen french-fried potatoes 3. Other products Storage practice 1. The effect of temperature in storage 2. The effect of humidity in storage 3. The effect of gas levels in storage 4. The effect of radiation 5. The effect of conditioning III. EXPERIMENTAL PROCEDURE A. B. Introduction Raw product 1. Varieties a. Kennebec iii Page 10 ll 11 ll 12 12 Page b. Arenac . . . . . . . . . . . . . . . 12 c. Sebago and Emmet . . . . . . . . . . 12 d. Russet rural and Russet Arenac . . . 12 2. Raw material . . . . . . . . . . . . . . l3 3. Preparation of product for storage . . . 13 C. Experimental storage conditions . . . . . . 14 1. Experimental storages . . . . . . . . . 14 a. Types and their construction . . . . 14 i. Concrete room . . . . . . . . . 14 ii. Metal room . . . . . . . . . . 14 iii. Haskolite room . . . . . . . . 15 iv. Tile room . . . . . . . . . . . 15 v. Panel room . . . . . . . . . . 16 b. Refrigeration . . . . . . . . . . . 16 c. Gas source . . . . . . . . . . . . . l7 2. Control of storage conditions . . . . . 18 a. Temperature control . . . . . . . . 18 b. Humidity control . . . . . . . . . . 18 i. 1962-63 and 1963—64 . . . . . . 18 ii. 1964-65 . . . . . . . . . . . . 18 c. Gas analysis . . . . . . . . . . . . 20 d. Inspection cycle . . . . . . . . . . 20 iv IV. D. Sampling and evaluation 1. RESULTS Sampling of the stored products a. Random sampling b. Conditioning samples Horticultural evaluation a. Inspection for mold growth b. Inspection for spoilage c. Inspection for sprout growth d. Inspection for any other defects Chipping test a. Equipment i. Slicer ii. Deep fat fryer b. Raw product preparation c. Frying procedure d. Sampling the chips e. Color evaluation f. Storage of chip samples A. Chipping test B. Moisture loss experiments C. General horticultural quality D. Flavor tests Page 20 20 21 21 21 21 21 21 21 22 22 22 22 22 23 23 24 24 25 25 26 26 27 V. DISCUSSION OF RESULTS A. General discussion 1. 2. 3. B. The ent 1. 2. 10. 11. 12. 13. 1962-63 1963-64 1964-65 effect of various gas levels on differ- varieties of potatoes 10% c02, 3% 02 at 34°F. 10% c0 3% 02 at 60°F. 2’ 10% c0 3% 02 at 50°F. 2) 5% c0 5% 02 at 34°F. 2’ 5% 0 at 40°F. 5% CO 2 2’ 5% c0 5% at 50°F. 2, 0 5% c0 1% 0 at 40°F. 2, 5% co 1% 02 at 50°F. 2’ 5% c0 3% 0 at 40°F. 2) 5% C02, at 40°F. and low humidity 3%.0 Control at 34°F. Control at 40°F. Control at 50 F. C. Moisture loss D. General horticultural quality vi Page 28 28 28 31 33 36 36 36 37 37 37 38 38 39 39 39 40 4O 41 41 42 F. 1. 1962—63 2. 1963-64 3. 1964-65 Mold susceptability of different varieties . . . . . . . . . Flavor evaluation VI. SUMMARY VII. CONCLUSIONS BIBLIOGRAPHY APPENDIX . vii Page 42 43 43 44 45 47 49 51 53 Table 10. 11. 12. 13. LIST OF TABLES Storage conditions and their sources used during the study Sample score sheet used in the evaluation of potato chips Relation between the National Chip Institute color numbers, their color and acceptability Color rating scores of Kennebec variety for 1962-63 Color rating 1962-63 Color rating 1962-63 Color rating for 1962-63 Color rating 1963-64. Color rating 64 Color rating 64 . Color rating for 1963-64 Color rating 1964—65 Color rating 1964—65 SCOIGS SCOIGS scores scores scores scores SCOISS SCOIGS scores of of of of of of of of of Sebago variety for Arenac variety for Russet Rural variety Kennebec variety for Sebago variety for 1963- Arenac variety for 1963- Russet-Rural variety Kennebec variety for Q Arenac variety for viii Page 54 56 57 58 6O 62 64 66 68 69 7O 71 72 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. Color rating scores of Emmet variety for 1964—65 Color rating scores of Russet-Arenac variety for 1964-65 . . . . . . . . Per cent moisture loss (cumulative) for Kennebec and Sebago varieties in 1962-63 Per cent moisture loss (cumulative) for Kennebec and Arenac varieties in 1963-64 Per cent moisture loss (cumulative) for Arenac variety in 1964-65 General horticultural quality of potatoes at the end of storage period in 1962-63 General horticultural quality of postatoes at the end of storage period in 1963-64 General horticultural quality of potatoes at the end of storage period in 1964-65 Chip flavor scores for 1962-63 Chip flavor scores for 1963-64 Chip flavor scores for 1964-65 ix Page 73 74 75 76 77 78 80 81 82 83 84 IloINTRODUCTION Potatoes are one of the important seasonal food crops of the North American continent. Due to the variation in climate, in different parts of North America, it is possible to obtain freshly harvested potatoes from July through November. However, from N0vember through the winter and following July, the potato supply should come from the storages of the major potato growing areas. These areas are, in the Northeast, Maine and the Canadian Maratrine provinces; in the Central area, Michigan, Wisconsin, North Dakota; and in the western area, Idaho, California, Washington, Oregon and British Columbia. With present day storage techniques it is possible to store good quality potatoes for the table stock market and have a very satisfactory product through July. One of the major trends in the North American food supply is toward an increase in quantity of processed food in the diet rather than the raw food. In the processed food area it would be potato chips compared to raw potatoes; canned bean products compared to dry beans and so on. The market for potatoes in the form of potato chips has been in- creasing continuously for the past two decades. The increase in the production of potato chips has brought about stress in the potato production and storage areas. Potato chips can not be economically shipped or stored for more than a few weeks. Hence it is necessary to manufacture and dis- tribute potato chips on a local basis the year around. The consumer has been educated to expect light golden colored potato chips and this places further stresses on the storage area of the industry. To hold potatoes in storage until the early spring months of April, May and June, and still have the potatoes come out of storage to produce a light colored chip requires special storage. The objective of this study was to determine if con— trolled atmosphere; where carbon dioxide and oxygen levels of the atmosphere surrounding the tubers are modified could be used in combination with controlled temperatures to pro- vide better storage condition for potatoes that are to be used for making potato chips. II? REVIEW OF THE LITERATURE Potato growth and harvest. The potato is an enlarged tip of a rhizome (Fuller, 1963). In North America potatoes are grown once each year. Potatoes from the previous year are cut into sections with one or more eyes per section and this piece of potato is planted in the later half of May. It takes an average of 125 days after planting for most of the varieties to mature (Kirkpatrick, et. a1. 1956). Physical structure of the potato. The potato (S. tuberosum) is composed of periderm, cor— tex, parenchyma and pith. Schwimmer and Burr (1959) describe the cortex as a narrow layer of Parenchyma tissue, underlying the periderm. Parenchyma cells high in starch content, lie within the shell of the cortex. The pith forming a small central core with radiating branches to reach the eyes is sometimes called the water Core and contains the least amount of starch. An average or medium sized potato tuber weight 6-8 ozs. Its spe— cific gravity varies considerably according to the variety, location and season.but the range is usually from 1.064 to 1.090, in Michigan (Thompson, 1959). Chemical composition. The chemical composition of the potato tuber varies with variety, area of growth, cultural practices, maturity at harvest and subsequent storage history. The average potato tuber (Schwimmer and Burr, 1959) consists of 77.5% water and 22.5% total solids. The solids are about 86% carbohydrate, 9% protein, 0.5% fat and 4.5% ash. The ash is 4.5% composed of 50% Potassium, 15% Phosphorous and small amounts of Sulfur, Magnesium, Calcium and Silicon depending on the cultural practices. In addition to these there are very small amounts of organic com— pounds such as phenols, chlorOphyll, solanine, lipids, organic acids, nitrogen (1/3-1/2) is protein nitrogen and vitamins. Of the vitamins the following have been detected in nutritionally significant quantities: Vita- min A., Thiamin, Riboflavin, Ascorbic acid, Niacin, Pyri- doxin, Vitamin K., Biotin, Inositol and Pantathenic acid (Schwimmer and Burr, 1959). The amount of Ascorbic acid is considered important since it is large enough to supply the daily requirement of Vitamin C. in some areas where the percapita consumption of potatoes is high (Schwimmer and Burr, 1959). The Vitamin C content varies from 10 to 40 mg. per 100 gm. of fresh tubers (Kroner and volksen, 1950). The ascorbic acid content decreases during storage. Potatoes have a fat content in the neighborhood of 0.10 per cent on a fresh weight basis (Kroner and Volksen, 1950; Lampitt and Goldenberg, 1940). When potatoes are stored for longer periods of time under light a green layer is formed under the potato skin, seldom exceeding 1/8 inch in thickness (Schwimmer and Burr, 1959). This green layer is composed mostly of chlorophyll (product of photosynthesis), and depends on light intensity (foot-candles) and time the tubers are exposed. The Optimum temperature is 68 F. Below 40°F. greening seldom occurs (Schwimmer and Burr, 1959). Utilization of potato crOp. Potatoes are dug from the ground two weeks after the vines are sprayed with Sodium Arsenite. This is done to facilitate harvesting and to set the skin of the tubers. 50-6OQF. stimulates the growth of suberin in the cut surfaces and thickening of the uninjured surface (Phillips, 1952). After this curing process the potatoes are stored at conditions suitable for respective future uses. 1. Chipping industry. In 1963, 23% of the total potato production was uti- lized for processing. Of this 46% were used for the chip industry, (Mercker, 1964). The chip industry has been steadily increasing all through the years and is expected to continue. Since the shelf life of chips is short it is necessary to produce them throughout the year. This necessitates ideal storage conditions of the tubers such that they produce chips of acceptable quality. Since the potato is an annual crOp it is difficult to get fresh potatoes all through the year to manufacture chips. Hence it is necessary to find a suitable storage to permit the chip industry to process all through the year, par- ticularly in winter and spring seasons. Frozen french-fried potatoes. Frozen french-fried potatoes are the largest single item (86%) of the total frozen products. About 70% of these frozen fries are sold to restaurants (Mercker, 1964). In this industry also, reducing sugars cause dark color in the fries. But this problem is not as critical as in that of the chip industry. Processors manufacture and store french- fries at the peak season of potato availability and keep the plant idle for periods of the year (Mercker, 1964). Other products. Other products which fall in the category of de— hydrated potato products are potato granules, potato flakes, potato flour, and potato starch. The operation of this industry is not year around and can be adjusted with peak potato production. Thus it is not too dependent on potato storage. Storage practice. All potatoes after harvesting are held at 60-7OOF. for two weeks, to allow for the cuts and bruises to heal (Phillips, 1952). Then these potatoes thus cured are stored for various future uses. In the post-harvest physiology of potatoes the variables which influence the quality of the tuber are: temperature, relative humidity and gas levels in the storage environment. 1. The effect of temperature. The influence of storage temperature on the quality of potato tubers has been studied for a long time. Phillips (1952) recommends an Optimum temperature of 38°F. for keeping the potatoes from sprouting. Heinze (1961) discussed the effect of storage temper— ature on potatoes. He concludes that at 40°F. potatoes could be stored and remain sprout free for 5-7 months. However, at 40°F. the potatoes accumulate reducing sugars and sweeten the cooked product (Heinze et. a1. 1955). Smith and Davis (1964) report that it takes four to seven days at 40°F. to accumulate reducing sugars and produce an unacceptable chip from tubers which produced light colored chips to start with. The accumulation of reducing sugars causes the dark color in fried potato products, since reducing sugars interact with amino acids in producing dark colored chips (Shallenberger, 1956). The effect of humidity. Smith (1956) reports that very little change in spe- cific gravity will be found if the humidity of the storage is maintained at 85%. Increase in specific gravity is the result of loss of water. Kushman (1958) states that loss of water from tubers occurs more rapidly than loss of dry matter through respir— ation, thus resulting in an increase in tuber spe- cific gravity. Miyamoto et. a1. (1958) reports, that more rapid reconditioning is achieved by using low humidity con- ditions. Miyamoto et. al. used tubers stored for 5 months at 40°F. and they recommend approximately 85% relative humidity in the reconditioning period. At this condition they obtained a 3.05 color chip after 7 weeks conditioning which is exceptionally good chip color. The effect of gas levels. Denny (1941) reported that the rapid increase in the reducing sugar content that occurs in tubers stored at 41°F. can be prevented by adding five per cent C02 to the surrounding air. Smith (1959) found that increasing the C02 content of the storage atmosphere reduced the rate of formation of reducing sugars. Burton (1958) reported that sprouting of the tubers could be totally inhibited by using 15% C0 and 65% 2 N2 in the storage atmosphere at SOOF. Smith (1964) reports on the use of high levels of CO2 in the atmosphere. He found that 95% C0 and 5% 2 02 in the storage atmosphere at 32°F. and 40°F. pro- duced “highly acceptable" light colored chips, for 7 weeks. Smith in 1965, reports that research on high C02 storage for chipping potatoes is very promising but does not give any data. The effect of radiation in storage of potatoes. Brownell et. a1. (1957) reports that weight loss in storage decreases with increased dosages of up to 15,000 to 25,000 REP, for Russet Burbank potatoes. He also reports that periderm formation is completely inhibited and suberization of cut areas is delayed at all levels of gamma irradiation from 15- 200 kilo REP, thus accounting for most storage rots. Heiligman (1964) states that treatment of tubers by gamma radiation in the range of 5,000 to 10,000 rads, inhibits sprouting and does not significantly affect nutritional wholesomeness of culinary qualities. 10 The effect of conditioning. Burton (1953) reports that holding tubers with high sugars, at 60-650F. for 2 weeks, would convert most of the sugars to starch and some sugars might be used up in respiration, thus giving an acceptable colored fried potato products, mainly chips. III. EXPERIMENTAL PROCEDURE Introduction. The basic objective of this study was to evaluate the effect of several storage conditions on several varie- ties of potatoes. Therefore the overall project con— sists of a number of tests in which potatoes were sub- jected to the different storage conditions for different periods of time and evaluated primarily by a chipping quality test. In addition observations were made re- garding general horticultural qualities of sprouting, weight loss and spoilage. The overall project was carried out over three storage seasons. The results of the previous storage season were used in determining the storage conditions to be followed in the subsequent year. Raw product. Four varieties were used. In 1962 and 1963, Kennebec, Sebago, Russet rural and Arenac varieties were used. In 1964 Emmet and Russet Arenac varieties were employed in- stead of Sebago and Russet rural respectively (these two varieties were employed since Sebago and Russet rurals were not available and since they closely resembled the Sebago and Russet rural varieties). ll 1. l2 Varieties. Kenebec. The Kennebec variety is known to the commercial world for its unique good chipping quality. It produces the best potato chips regarding texture and flavor. This variety was readily available at the time. It stores well, and has an excel- lent process rating with high specific gravity (Thompson,hl959). Sebago. The Sebago variety is not recommended for chipping after winter storage but is very good for boiling and baking. This variety was chosen to contrast with the variety Kennebec. It has a fair process rating with medium specific gravity (Thompson, 1959). Arenac. The Arenac variety was developed in Michigan for high total solids; it has an excellent process rating with high specific gravity. It is a late summer and early fall variety (Thompson, 1965). Russet Rural. The Russet Rural variety has a long history as a chipping potato. It is a round russet skinned l3 potato, capable of producing high total solids (Thompson, 1959). Raw material. The potato tubers for this study were obtained from the Michigan State University experiment station at Lake City, Michigan. The potatoes, after they are dug and cleaned of any adhering material, were packed into 50 lbs. paper sacks. These bags of potatoes were transported to East Lansing to the storage where they were held at 60-750F. in a covered condition. (Light exposure of the tubers induces the formation of chlorophyll which is objectionable since the green color impairs the culinary quality of the potatoes.) They were held in closed containers for 15 days to maintain the high moisture content necessary to help in the curing or healing process. Preparation of product for storage. During the storage experiment the potato tubers were in field crates. A random distribution technique was employed in transferring the potatoes from the 50 lb. sacks to the crates; two tubers at a time were re— moved from the 50 lb sack and placed in each of the crates for a particular experiment. This was con- tinued until the sack was empty; in the same manner another 50 lb. sack was distributed. Each 50 lb. 14 paper sack was distributed into all the field crates used for each variety. Each crate was identified on the outside by a label giving information of variety, the storage room and the temperature. C. Experimental storage conditions. 1. Experimental storages. a. Types and their construction. ii. Concrete room. This room was made of insu- lated concrete panels. The inside walls and ceiling were insulated with 4 inches of poly— styrene foam. There was no insulation in the floor. A blower type evaporator provided cooling. The gas sampling tubes and thermo- couples leads are inserted through the wall at the back of the room. The front end has an insulated door. The inside dimensions of the room were 6%' x 3' x 5' (97.5 cu. ft.). Metal room. The metal room is in reality a wood frame structure insulated with poly- styrene inside the outside covered with galva— nized metal sheet which acted as a gas seal. This has an Opening at the rear for the thermocouples and gas sampling tubes. A side opening serves as the gas inlet. The power iii. iv. 15 cord passes through for the evaporation in the rear. This room has double doors. The inside metal door fits into a groove which is filled with petroleum jelly. This door provides a gas seal. An insulated external wooden door is then placed over the metal door to provide a refrigeration seal. The dimensions inside were 6 x 3.25 x 5' (112.2 cu. ft.). Haskolite room. This is constructed with "Haskolite" foam filled panels of prefabri- cated polystyrene type material. This has an opening at the rear for gas sampling tubes, thermocouples and a power cord for the evaporator. It has 6.5 x 3.3 x 5' inside dimensions (100.65 cu. ft.). The insulated door of the Haskolite room is gas tight. Tile room. This is a double tile wall structure insulated with palco wool. The in- side is covered with thin sheets of aluminum, which serves as a gas seal. This has an open— ing tube on the side which serves as an in- let for gas. An opening on the other side has sampling tubes, thermocouples and power cord pass through. The tile room is equipped with double doors. The inner door is the gas 16 seal door and fits in a vaseline filled groove whereas the outside door is the insu- lated refrigerated door which is bolted in place on the outside of the room. The in- side dimensions are 6 x 5 x 3.5' (105 cu. ft.). v. Panel rooms. The panel room installation consists of five small CA chambers inside, an overall facility that includes a work room and a 40°F. storage area. The panel room unit is made of a sandwich of 4-inches of flame retardent expanded polystyrene (Styrofoam) insulation with %" Douglas fir plywood faces. The corners are sealed by in— stalling galvanized sheet metal ells and filled behind the ells with an architectural calking compound. Each small chamber is equipped with its own evaporator and has pipes for gas inlet, sampling inlet, thermo- couples, and power service for the room. The inside dimensions of the individual chambers are 4/k 5 x 7.5' (150 cu. ft.). b. Refrigeration. I All rooms contained an individual evaporator fan. 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Sample # Variety Conditions Flavor Crispness 1 Kennebec 10% CO , 3% 7.8 7.2 02 at 40°F. 2 Kennebec 5% C02, 5% 7.5 6.6 02 at 40°F. 3 Kennebec 40°F. air 7.2 6.8 control 4 Sebago 10% C02, 3% 3.3 6.5 02 5 Sebago 5% C02, 5%. 5.3 3.0 02 at 40°F. 6 Commercial ---- 7.0 7.6 unsalted chips 7 Sebago 40°F. air 2.3 2.6 control 8 Arenac 10% C02, 3% 5.0 6.2 02 at 40°F. 9 Arenac 5% C02, 5% 02 5.5 6.7 10 Arenac 40°F. air 3.0 6.1 control 11 Russet rural 10% C0 , 3% 3.6 6.3 02 at 00F. 12 Russel rural 5%.C02, 5% 4.3 5.6 02 at 40°F. *Average scores of six panelists are given. 83 * Table 23. Chip flavor scores for 1963-64. “—1 Sample # Variety Conditions Flavor Crispness l Kennebec Control at 6.1 6.5 40°F. 2 Kennebec 5% C02, 5% 02 7.2 7.5 at 50°F. 3 Kennebec 10% C02, 3% 02 7.0 6.6 at 50°F. 4 Kennebec 10% C02, 3% 02 7.5 7.7 at 40°F. 5 Arenac Control at 4.5 4.0 40°F. 6 Arenac 5% C08, 5% 02 7.5 7.1 at 50 F. 7 Arenac 10% C02, 3% 7.8 7.6 02 at 50°F. 8 Arenac 10% C02, 3% 5.5 6.1 02 at 40°F. 9 Commercial ---- 7.2 unsalted chips * Average score of twelve panel members given. 84 * Table 24. Chip flavor scores for 1964-65. Sample # Variety Condition Flavor Crispness 1 Arenac 5% C08, 1% 02 7.1 6.5 at 40 F 2 Arenac 5% C03, 1% 02 7.8 6.5 at 50 F. 3 Arenac 5% C02, é%.02 7.1 7.0 at 40°F. 4 Arenac 5%.C02, 3% 02 7.5 7.5 at 40°F. 5 Arenac Control at . 7.2 6.5 50°F. 6 Arenac Control at 7.6. 6.5 40°F. * Only the author's flavor rating given. @Low relative humidity (80%-85%) condition. HICH RN (ml/1mm? n E UNIV IBRQRIES I)UNI/111111111171!!! mm 31293106656964 III