Sex-specific effects of developmental dieldrin exposure on synucleinopathy
Human and animal studies have shown that exposure to the organochlorine pesticide dieldrin is associated with increased risk of Parkinson’s disease (PD). Previous work showed that developmental dieldrin exposure increased neuronal susceptibility to MPTP toxicity in male C57BL/6 mice, possibly via changes in dopamine (DA) packaging and turnover. However, the relevance of the MPTP model to PD pathophysiology has been questioned. We therefore studied dieldrin-induced neurotoxicity in the α-synuclein (α-syn)-preformed fibril (PFF) model, which better reflects the α-syn pathology and toxicity observed in PD pathogenesis. Specifically, we used a “two-hit” model to determine whether developmental dieldrin exposure in mice increases susceptibility to α-syn PFF-induced synucleinopathy. Dams were fed either dieldrin (0.3 mg/kg, every 3-4 days) or vehicle corn oil starting 1 month prior to breeding and continuing through weaning of pups at postnatal day 22. At 12 weeks of age, male and female offspring received intrastriatal PFF or control saline injections. Consistent with the male-specific increased susceptibility to MPTP, our results demonstrate that developmental dieldrin exposure exacerbates PFF-induced toxicity in male mice only. Specifically, in male offspring, dieldrin exacerbated PFF-induced motor deficits on the challenging beam and increased DA turnover in the striatum 6 months after PFF injection. However, male offspring showed neither exacerbation of phosphorylated α-syn (p-syn) aggregation in the substantia nigra (SN) at 1 or 2 months post-PFF injection, nor exacerbation of PFF-induced TH and NeuN loss in the SN 6 months post-PFF injection. Collectively, these data indicate that developmental dieldrin exposure produces a male-specific exacerbation of synucleinopathy-induced behavioral and biochemical deficits. This sex-specific result is consistent with both previous work in the MPTP model, our previously reported sex-specific effects of this exposure paradigm on the male and female epigenome, and the higher prevalence and more severe course of PD in males. The novel two-hit environmental toxicant/PFF exposure paradigm established in this project can be used to explore the mechanisms by which other PD-related exposures alter neuronal vulnerability to synucleinopathy in sporadic PD. We also assessed the expression of total α-syn, DAT and VMAT2 by western blotting, inflammation by TaqMan Mouse Immune Array, and levels of dopamine (DA) and DA metabolites by HPLC in striatal tissue following developmental dieldrin exposure. We found that while developmental dieldrin exposure had no effect on total α-syn, DAT or VMAT2 levels or on striatal DA levels in both male and female F1 pups, exposure produced distinct effects on the male and female inflammatory gene profile. Finally, we also characterized the α-syn PFF model in female animals for the first time. Our results showed that female mice have reduced loss of nigral DA neurons and a behavioral resilience to the same level of DA loss compared to their male counterparts. This is the first study to show sex differences in sensorimotor function in the PFF model and suggests that the PFF model may be a valuable tool to model sex-differences in PD pathology and etiology that does not require any additional surgery or other treatments to manipulate hormonal state.
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- In Collections
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Electronic Theses & Dissertations
- Copyright Status
- In Copyright
- Material Type
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Theses
- Authors
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Gezer, Aysegul Ozgur
- Thesis Advisors
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Bernstein, Alison I.
- Committee Members
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Sortwell, Caryl E.
Collier, Timothy J.
Leinninger, Gina M.
Rockwell, Cheryl E.
- Date
- 2020
- Subjects
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Dieldrin
Sex differences
Dieldrin--Toxicology
Nervous system
Degeneration
Neurotoxicology
Parkinson's disease--Pathophysiology
Synucleins
- Program of Study
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Cell and Molecular Biology - Doctor of Philosophy
- Degree Level
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Doctoral
- Language
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English
- Pages
- xvi, 169 pages
- ISBN
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9798643188650
- Permalink
- https://doi.org/doi:10.25335/d1py-rd60