es —_— THESIS Influence of Temperature ! and Acidity Upon the TR aS co R.L. Brown. 1293 | ee ST ae ae Ce etal aie FEN LEO, a ry THESIS oT A S PT UD ¥Y QO F TOs ITuUPFLUEW Of “oR de of hmgIPERN rp vw I tT H RPACILIUS ACIDI tbAcTiIicdl. e ng D Brown. <= . | Ot wy -adohican Acrpicviturnl tollese. THESIS A Stuecy of the Inrlueice of Tennerature Ana AcLGLty upon the Guslity of tle Vutsex sere e Don Flsened “ith x rf "aOCllins ACL CL Lietici. Tae Ougect or the exyerive.t is to dete vrdine }4 bw ra +4 TW : ; _ am . = 2 ° . ° - ~ oO Case Or X Pacli ius acidd lactici tha dest Ter oraturve f.q the rost suitable @mount cr acidic > it 3, - os ° ~ 7 . I. an on 78 e « Prey Ve Plpening or cream in the vrorirsction of the Te purpose vas to stuey toe ervroet of tow rerature and acieity on tite anality of the vinish- ed orpoduet. Tee te, Leratvres at which tho cresm -ag wivened were within th: rinse of 60° to 30°F, The acisity wes studied only under tae .ost sutis- Caetory tomer ntures dete ined above avd in variacle Th order 710 “2at cretiin Tree voir tirrev7oices thet mic:t crise »srevious to tie experivent, it ~ + 3 4 * Cy 23 wy 5 an wee . aos * " ~ " wag kot adn one vat asd well mixed until civicted Por winouning. bv Nae see C ar a - vote ST Ce . ° : ‘ wre oN ° 3 ed with come atarndard com. ereial ctarter Was aloo : : 7. oe wee 7 . re used as a iorther cheek wpon tre vorwk. IN was . <° - ey ~ +; wre aX eercagra series ; WOW Savor 9 wILG COLIC re 4s. a] Sstarver Was 94272 a oy . 4 “hw. . . 1 Pe .. OV boved ad only the petureslly rivconed cheek was Peo eosults ov the west vere determined a - ~ ~ - . ’ . L?Om Stores Wsae by son Re le, 4 4.4f4% ’ ee _2 OD ct -—~ < ve + . Mite 4 1b Putter guises. Tho 7 PVE Seere was mace soon atter the secora workine 1 | 2eK5 ane Te setod etter otering rer ronr weeks The Etarter, mTra X Tactilus acidi lacticli used was @a reria Serover a. 40 vrotuced a food virm curd free from 2as bet oseive ae rather stwons, earirel Flavor. This Llevor sw es . ~“ ors 7 | © ~ . oft ; ,7 “ss ; l vy, on we Ty . 7 7: ~e - Ves cot resraradet es €ot~rivonta LO Tie CHES L art S a good one. The starter was prepared by steri- Sate ey Ae Sm PJ ele ws Fy. 4 . an lizii ag ilk an Tlesks havin: cotton wool stopvers, vo era tion anecul.tirs with the desired culture, Tho qeeam wee tevion either rrom the milk of tne colloge herd or irom thet Vought of I. A. Smith, cert. - Un ae re a ae ma 1. 3 OL TaKem. moh churnire covctested of ey or . . oe kt ee Vy ee Oa, “AN OOO hey iss PAG 2) WwyA0 S 9 7 i boo 1, a ‘ , 3 ts t> Us . 4 ‘On éA J a a 4 a win, >) oo Ce t e me ‘on: t, ahi man easy cepyecbocon wes taben os one smuple v7om the ornaa vat, thoroucnly mixed and tented for fat 7 ji were 7, + + trsqeenoole a portion was put in a a | aA Fr = >} TN -vy “NIA . . mid acid. Prem tals cee pie can .or naturnl ply ning. The pe.iwinit: eresm was tren thorousvhly prastuerized -9A 200:74 70 the PL ening temLoratvure. A Tixed acount vas then put in esch of tvo glass cans, ane inoculated, one vith KB. acial ‘actici starter, an7 the other with one Of the convercial lactic acia siarters. The thrse cao, with the covers placed on loosely, were cut rater «t the desived tenverniture of rivoning. “With this arranze- rergt atowas fourd corsibie to koa ny tre terrverp. ture biG Poenwe er p2ive degrees Ler wrenty-rour See eresiiowa. cooled a4 cimvrled. This vas cone oy eens of @ eving ern avranced vor chvrning tou ty pee senples.simltanecusly. This wes devised ‘ifferverces in churning, wut vas conned weovatistactory, «9 in the letver uart of Z -~ a - e - . . 4 we ” . . . - the excocrirent chatins vas resorted to vith cetter . rt rosults,. ; . ; oo) — brs ay 4. eyed olneed in the rotrisernator to “llouw te salt « - 3 S om oe . * yy oe - ay ~ * iq then pevoried and printed, Ua. hftew wicaes tis: to horden,it was scored Lhe ene Soowrtly by iv. don iichels a. 20. oe. Ue l . s : ~ . . . cus ~ 7 . Nc\ yy UA t a t, ws © , . aot ko putter owas wp yp eed GM Porc. et uo gest TN Gy + on ordizepy eorricorater ver cur wy eis usiti wT fi Oro oury inca Gane Vso we NN cee L oni fof .. oye Pa 7a Sarge are oO CT tl T ma ‘ . ‘ ivr we OIA O° oN ~ c Nota a wilh uc aX\4adeu Clay Liles l, pte a MS tr, .! “¢ me a Pola ae ary O° . -~- 7 my wee yte ri (9 oO A Ss mrs Vou =] aR 7 a a: cy YR te oN ON Lh + an oy eo. eee Le “ ae . ° lever wan teen «5 the iin basis or conmuari- « ' ° ° 3 2 -. . reer ae - ’ + Str tre, a ~ res cf re) asa ‘ SOT. . . C LOR PaUtiPgs ot SOL T. a ‘om TOK ae CAD ~ . * . ewe oo - aury 7 i. 3 - . - + yen wo + yr 7 oe - oN rer OF ma) ao * CI Le EY . i . thy ‘ YOR. > Pore iO? CA vs on. e Cg ub wld ’ C4 vy? a. Las fh QO ! . oe V : - ” Cae ee . yong - o. . a st Old acsiee that tempor tore in a Saeort s1.e. m.: ney? oa rm syor + yep ys yep OTs ws Va oo, WA To. ehance of tomperntire serblpg Ph emaiy bs p.ven * : . 7 - 2 oN zayo7ou et sranwt gy _ . -,7 a7 vy .eF 7 No, oy + 7. ‘5 . vo mT 1 yy (* : t% RN LO . ON, ail. Jefe LV ple t, Vitae dh he WR EL ’ OO. L ..¢ . + « + “ay = at . om 4 + 7 Cy “ oe + wr pT c 1 > Oo 7 . ” =) A e a vor t a 5 ( ~ ¢ mm... Vea 1 oo oy : a°N Gq wea yas “Pp x ead ‘ aS A y oO re oo 7 ‘) | ‘3 u C J , L,3 .y 1 “eo > a we Sell Ne te A < Potains _ goidi lactici (.), Keith (K.) Douglas (D.) and - . . : e . . se ~ . oe an 4 Vy oy a Hansen (Il.). The x BR. sciai lasticl b lig tee basis aL ae _? - . . 7. . La A RATT Cen. OF the werk wes une in ©8ca trial aor tia corner oS - * “Nowy v . - «ee ~ 4 ~. ce . ’ . “ \ os ~ : "Nate ] {~ .s y : ns : : i 43 4 at, sara: . . a \ \ pf 78 A ON RRS Tle est Tem err tics PAP SBR pF oe ~~, a % .§ . Nn, : . x 7 e\ . o f . i a haieanels rmaryyeen a) LSt Prd lst ond fr» “rx wv "eS " FQ wh. @ AL © 3Q tH 4Qo om ee om = « Ap "GR" Vo" 93" 64,4"K " 46" se" eg oH gg” A; * ep" | a OZ WM ar BI "ogaa m BEM Az Wooo M BR, "ogo " ae om oO Ww BA Woe moen7 woe th eg wom om E L " a 9 ! Q? 't og " FE 2 t! hi. ] Z S " _ tt 9 G " =~ ff R, "59 " g® Ww og t Gg tho 4o " = wan om tt C, "oee ae mesg MBS Wie th Aa Ht AO OM ZA NW AE " Xi om moEe MN °° moog tM BL Wh. tao tw Ay," A244 " 4 Cs neege uw oF tH CG Nt Za to " ZQ " 49 NM AD HM 40 1"! D, " 6] " 1° t 24m G7 Why wm “sD " Aja" ze " 4D mM De. " 61 " 1° " OA eM G1.5"p. " 45 hao wm 4g ' 2G tM js " 61 "1 " Og BG. .5" 44 " az" 42 NM Be Mm , ) wore i " g } " © 2, w Fr, 5 ! hf. " Az " 4 0 " Ks 7 " 5, z t! FPF. " 67 " G6" nook "(65 ) "Th mize uw Zou 28 " 44 °+%N Ks " 5 noo woz Me” tt i boa" 47 Woman mM y me A num 7 ON 70 moe mb oa MW GA mir th AM ZG" AS =O Tr, v 70 " Gg? hoon 1 71 .5"- ? 20 " ZO 1] 4 " 2% tt ~ 4 € { G " 5 QO wt 1 '! c G tt "7 C tt ye { w 2 2A 2 4o " 35 " SCOMVES ont On Pa We me, " &9O ! "! XK BewUsiel " " ineticiM"ss 3" " t “O o8reted "agar Z/ _. ae POM Galas ~ samen Mie entien orn beh A pOnGgaw woon the . ~ yoy “yy 1 ek ‘ _ Ce rovuit thors . - c las —~* @ 7K S1ive, veciwee? OF ts . > soa Te, : Olt obther 1 ade erature were +7 Sy : « ~ wre . ; ’ : rT 1 we wha to . oye 1 wo. . te: . a? t Uaeal Matas 7 e Lay rp a~ey ey +. cits. cle. yo ek 1 an keg “3a Wi ee fo mw Cla *¢3 C “ture, into Arran: Okt EPO, Flavor Alone, Ssovre, socond & " G6-7 "60" SO " 790 " "t "! tt " " vw 48 3/arse" tz z Woeeze WM ome 4 39 o>) 27 oie 2 ‘eae, od AQ e " tT " i" tt 43= ! " "ot nao 2 q- tot oma 4] “ay 1/4] “gn SG e ms ' a3 6) 22 "27. © - - a a 47 ~ a? ve oN oon - me bet + QP On OF tc a. OPa ccs a pear Sa oy yo flavor vresveedq as -- tease. en > oe - ay 7, Coroeore tures or rlivening. Vue “tive entirely conclu- . mayeeni ye > tale OQ Ji.oaite a muriw of trials. “ 4.7 - ye 8 . “ow 2 tho Vip t or sacern seri _ oe vo geet a | f iis to hrits out tre weerlority or aby once tom- wae Ft Wwapyr.sy »n\F an? coPpauture betweon GO and co FT, Fore Torth ow cowparicon, enetier tanle of avor- aves wes ve rored sirilar to tie tipst, adiclo ing ‘ - ” ’ ~ 7 ,f y 7 a . +706 tie hexty seeve with the Plavor. As an wir wee Os Cote, tas dirroreuess In tne Ss 1ope are noetoeupouisa ont to indiente a suvpricrity or any one _7~ Wawa te ye -- cs 74° , ~ ~ * AME OF “COP IS, Arranswed Ac COmPriange to Tornerature 2 ; rr 5 a Grn the “egis Or Flavor aid Tex + Wow wii First, score Can m ' ~“e . Oa. SOACTE, ’ yay “\s06¢ Ha yey ot nN ~ . n TOR) -OPagiire, OU "79 "36-7" 69 "5 " 70 "65-7" 69 " x TRoAeiAd " tt " " " " " tt " . to : . vw . vr 7st —~ » ~ sos “we Paoticl GZ 66 "G7.6"65 "64.5 "GLIE"GGLE"Go 9" " " wt tt w " " "! een . / an “wen 7% rN . Gov ercial "70 "71.5 "70/y "75 %"S "65 "70.8"GG.E" tt tt A} " " tf ! vt ! tural "72 "6S "GGe "7O.4"C4.5 "EG 5"66.6"64,5" Tne result of tlhe ex verivent, tren ais that Shore is «et least no .ercied suvericrity of a cer- Lo vane wee om TQS ges 9 = ...8 + = rx on oO x “> m7 IN % * 7 ot ; t : 0 r+ SD Ha ATT We tTALr the Lliiits of 6 ard co F, - - 9 yas: yan ow ~ qr? ~ -. 7 —_- So ~. NY - . Crews wieened at gu as at GO FL, 1f provevly vianazen, . 7 ~Aas -y sree ~ an : . FN ve ; ms syv4 hs Tris mist, however, oo tauen with tae Llisitations a. ae seat yeas ayant} yy werya sy, ve 4 mo i. — OF Dole OM oPaneny, vom we RoWEtn otaer ceris or _ i a = aS ne . S- Qa = pase cay Laintodr eream misnt produce reteurble ily disvrerent “= 7 ™~ :” , 4: r NO ™ we . eH ~ an oy + . Pe cwulLas. Tp Vibe A ]l Sass we os “oe at rial cn onze ney ghovs a proverence “or chy bperticucar tenvore ri dime Sanaa omer Cawsy os a wt lw LOG. Peo ob ooninge temveratures in the secora series 2 5° -— 4 q v <” 7 9" coe +2 7 . 7 , ars were c20cen Petiween 6 and Te Pr apace eretis wore e & -. wee ‘) | -- + grranceds ans ri,oned =t 60, ore St 63, oli Ome a . ’ os . - cite >, +: 7S. mn Vey ecin at 727, the evorm was tesen Tron tic %O .1eKe ava hiy rr Xen , ested Por fat and acid. a 4 -_ a) ° 7 o YA Stary Prana owreze “7d aq Tr EAI Hie TUG Cok pS ne a EO TT) OR Geka bic le LAC? Yoe coop cheernines., Ore can vas pastueriged and . 4 wep oo ’ ‘ c + 344 ao? ~~ 7N, “ inceulated voc A XP. Geied lnctici, the other ale ricener 45 an tie wreviou. part ov The Cleon wes to mectea tao onivescs ata Pom a c46@ ° HVT Boe sp et, —, are. paged gs . aii Ord Va. ~~ aleb Gh 7, - - LPom Corie tne can POXL ~ ely oleilar a ~ ° Lad a 4 7. ao vo row each, wxecpe tia oact “oss ~ + - a | . Veyar cy we se - i SO] nection with tre develovinent ++ ry Yr ‘ { Vann : . mrt avorrdies or asin Prom this rol HS in the px LS syare ; ta he CAVGPL:. OF: Ge ~ ; v7 ~ . ° e avTeront acidities SUCRE Tez ent ehopnoad, COnALtLons ~ b 7b eve aiven in oi lke Forward 41S Sf t~ ¢ <4 I | - wwe ay SD wa \ i ne the Sart +74 TAVLe or R QQ ys ° ~ 8h pe “9 e rol 0 ~ ¢ 2h Oy Tr oO NEY HO c y ja. : e a S134 1 rT) Dy " caval ha e/ 6.2 eet Ve 2, "62 " 3 hi °/ " E & " 4. "62". . . mn ¢ ht Z " G 5S " °e J oo OP ii, "6B " Ale "$3." my oy re it Ling "72" 452 2 ie "76 " - t 4 Kid " 7 2 % 4Mz "72 " wT "OR " oN "Ep 3 MN "en " A N, "65 " wean "65" (31 oO 7 "gs " 2M "GS 4M, "Gs " " Zz or Ny "TR MRS | neo MM 2 M5 (© 2 Ww " 7 r " e 1S a 7 r " 7 s " \ x +-TJZ3 nt EGsvltsoqret.e charrri:sg: at bivrerent AciALtLes. CC¢< co. 7 . - oe o — “Oe _ _ Licnels rerewson oe oy 5 ay. ArnmxKX. “FAM. WAL. lst ol. 1s rr? "w a ‘4 2 ' ce . — Co. —~ DEAS IEAIY Shiga SCOre"score “"Soore"scere " tt 1 " it tw it - T OM 4 2 4 1 4 a 3 Oo tt ‘CoM i" 1" tt moar 1" ° He) 40 ag 40 4p om " " non " 49 " 40 ozs " Al rT) " " " 60 WoA® moa) nome it Aw " " " " noAdL "zg no2g " 40 1! "4m om " moe Wo AO n"oAd " 4O " “nN 9g noe " oy " " " noe? " 414 29 £6 Ly! t 2 " i " " 1 rg " 4RZ " 2S ' fe 40 oo an " 7) " " no4Z noe Wome " 23 } r tt - tt moO " "ABM " "4," 37 35 33 - " : " i " tego ot 42s " AQ " 43 41) ° - cd 4 m0 i! " Gr " " " "668 " 4le " Bey 34 5 novo. Ww " | " -_ " " " e 4 8 " ” AS 3 7 “i “a et ' 6 c 1 , i" tt t 66% mo yo no7G t sO Ad. ‘" w " a t 5. 2S t ! r. " 59 "445" 42° " AB AS, " " "31 moa A noAA Woe " £% " " i" 1) moOAS "29 moa] " Le ! - 1 mo " " " 1 Et "oA "o2S " Al " vw . 1 2 tt A(t -~ &W | z " 77 " LE "! oN er ay — > it " " iT} nmoAG "o2G nos " Ze "oo " Ao " ym fom ' nmoAyz ow ay 24 an a “262 “ . co (ad a 1 7 ' én! T noe gt "4A RY ad oJ _— . noaz ew 4g" "! "Fett moAy-. " 40 re M4 ese — : a \ ' ~ “7 a8 - ~ ' my 7 eo “4 * ’ ” 0 ‘ aoe 1 + we ee CSS ye te PO a TP OO cy Or BOA . 5 { 4% 7 mre -:° . ~ ’ vod y Oy 1 Jy a : ¢ OSs 97 1” oF Ve Ol: ier > L Payan alone, ° oo - 7 n Ly! yy oan ra ow moots cl atte Pee 7 v- x . a ak - as howe - fo ef ae Ae QO panitliteste ow, 7G mn? ry CO 4%, mur - sere a = am vA ‘ - 9 mmison, *he avery s were tion arrunged la oemop a) . . ’ . . . oh 200O™ 3 0 the ‘omens am 1, et TBA Ts We | ln {* r 6 pie rs ri coca yt ose . . 5 - Le OF AVOPALOS , GOL Chr es wlos OY, tine 1 — a “¢ “ . ‘ é ‘ 7 r . a7 sf ragig ov Tilavor, © 7 Vilawoe cid Texvuue Lyd ee K be,dy.Cuncd Aciag Toehor, A sacs Cor. yp oy te a oe OED ava A as FE e a wt 4% tt oH - OOD “op Se TTY OT, “wT ~y7e ff Lue Pa v of Ome’ COM oe EU Comets oe rae TA Pawamn " a " 2 f TIlAVoOY ZO lL 2Q 7 tt tt 49 63 ! WI a * ones { ar * am 't \! aver C1 . ad ' Fy / e 3 " C3 " " (9 QO es Texkivre o wary CR RAN) - WOO AY Tf ba 2 e rT JVeorunwm tt wm W ‘ Ai AT (2) '! Lhavor eo ay.7" 49.2 -. N ~ a +r r co TIisysy mo tt 37.3 " 37.8 " " 60.4 7. eye a SPs 2 payer Wa anya se typpal mi ONL ae mT a a ory , ia u "PEG So pe > we ~ i mT — -_- to AT TT my 7 Wo 7 ART a rAN c oN AGLTLUY a om eo Om AD ~45-.54 257 eo fi 5 tt v ro | on tf AG 7 ! v) " Tilavor £1. OOe VU “rg ee) ba . a — ry tl oy 1 i re ! wy iy 2) it Tiayor avd " 39 en 537 70 et) ae er? ~ os X HUY ~A Mog gad Fy el uct. . _ er YW — ~ i! Z t i nd CO " 2 riayver n 30.4 39.0 59.0 £4.5 Rlevor ard Taste Pe Pre wMaswit ot Loa arjyALt7 es . a , Cuts ode Lee 3D 9 aoe lities. woo my of Ww mM a, q ‘fr a m " Wo se Aa gs / e oJ) Of e a? ere et? . , wee ywyle ~ - ~ oy a mw < “yy “e -* a4 ,* - _ “y +, (> ve cy a hoy in Leet ly wed Lik wed, O77 Faia + 4 ‘ 2 “ o ° ~~ mow -} Ver cet sot “s ethoas to Plavoy Se spats - 7s +! soy poe 7 Pn - ry Bde EN : Try rant O).. i © ‘ t, 20 a: Vie oi” 1 oe 1G. > os ks Ct a — . ON, . - ’ - oe ~, Sold ones 2. elove tis ae dh ; . . 4 an ee a“ anne Oo _+ . ry ®@ WwW) Mt t, : ol OWI STE TZemonits ’ CTs ~ Cl. . “> . @ 4 . Pmt on 1 an wee eT tty or C @ iy sal L a Gael Le - us Rowe ke ad .) iO Jlayvor “FO SUbeeorier Speeceirie wot wk EEN ~- 9 - -~ Roo we Loree mee eS DT Se Parre of 435. to Pebeliwy Gn whe opscuw tie Vvlever too f° Gi dol Upeemetoves oll lt ly divpwoved. Ne vw od o ry 50°R., - ay a 7 - : res aD “477A cee eV) aN cry aooni USe Oisl? ROOM USe Oil line F. - at be le 2 TiN 1528 — a r