THESIS i i Effect of Bacteria on Bonemea! WR, Wright Teley THESIS XX 3 Wie, _/ 4 4 . a (os THE, RFPECT OF BACTERIA ON RONEMEAL. ( A Partial Verification of Work Done by Professor Stoklasa in Germany, ) BY vw. Re WRIGHT, ae, Michigan Agricultural College. 19 0 2, Tie ein of this thesis nas been tne investigation and noesible verificention of some valuable results regarding the erveet of bacteria on ponemeal rertently puolisned by Proressor Stoxlasa of Cervany. Ti? subject 18 one of amicul- work tt. toeral intorst and vorthy of esverul consideration. Hi VAS MVeLished in Sete ti rpie avd fhoricnlitural Jourmmals in hWurone. Tt has not cocen po. sible to tike up tae vork us exten sively as that carried on ty tne Garrian Profes-or, AS his WORK sould be reaiily divided lito tyro sxrerivents, one a verification in a horticwltural way of tie other, we cho-e to qplicate tiat part most intbiately co cted with the J eience of Racteriology. PFurtiiermere tie tine allotted G20 1.6 periat of satfisractory revetition of the entire exp: rirent. ceiving the cereals of our own work dt will ba 4 Neo! Refore an necessary to present a few Pacts vresarding tic rroressor's work, “ ich weve y 4 O443R74 or the six micro or.cnisms an soltition as follows: Ten yeas of ponemeal, (analysis incicateda the presence of E76 2or cent of nitwogen and 19.8 Uuecr sent of Dhesphori¢e acid), were put anto 900 c.e. of Storile vater, ind iron sulvhite, potassium sulphate, ana bagressiwa chloride were aaced in sal quantitfpies. hqual QMVrectangeos waxes then put into sevon flasks, one of thase being retained as a control. ATt P inomilation tire Masks vere reut at a tomoor tire Pavoraole Pow (ae. seovth, After 35 navs iad elapsed, They were tnalvazed, vith tie rollowing results: hivrosen, Pnosvnoric Acid. “Sanrtrol flask— ee — —3.695) 0.76 (2 boraterdLium —-—- - - = =- -—- — = 4.98); A.Oo7 @ Fs: 7? v ~ , ue » _~ “ - . TLOreES Ligief.- -- - - - —- 1.9% 1.08 ¢ C2 Prot ogee Wlearis-—------ AEG) 2.93 G Mvcoides - - ~- ~- - ~~ + ~~ £.16:. 4.56 & lies@ ntericus Vals«atis- - - 4,785 4.08 G An exauinstion of these tulated epesnlts sieecs us: Peat Te evrect on Phosvno in Acia was sreater Tien on Sditrohen; the ver cop toos Hitrovwen very var less tian those Of Phosphoric Acids ard teint in «11 eases tle sors have increased the cnount of Yotn olerents, ov) , VreNnayye? raed s “A 4 76 a: met Map eyty dT <, In ou,T will Mk, PRs Peay, t Ore MAG nov, available, 70 Old eal vas used, Thies enh tontedeced ioe In sall qoanti-~ tiles, v.ch facet propabiy assounts for the ssall cuanges Lat occurred, To piderstan? tie vork thoroughly, say oF tio ietalls Mist wes given. Uarshn lA, 1908, seven 595 c.c. Erlmever flasks, each containing 11/2 sruis of tic bonencal, LA 94.0.0, oF Scorile vater, © Jicile potarvsium sulnhota, wage nestum chloride ci: avon .ulpnate, were prepared. Tio POLLOV= Tig dav oaey ola were Lntrodined Liwo Piva or Tew, toro edie hoserved as scointrols., Thev vere placod in tie in- onater room, whore @aounivomm temperature oF 25 C. a8 “alin- tadned. A®torm cle expiration of 23 days these vere »e:oved and “itrosun wil Phospnorie Acid detornunations made as rapid Iv as pocrsivle., The Kjeldahl = ethod was used Por deterwining too totedk asourt of Vitrogen, soci the Uranium titre ting pothod Paw toe Phosshorie Acid. Dunlicste tests were, in All cases, ade of the contents ef cad VLASK, Usd in two stances a disagrecsment iiaie it -esessary to repost, .ocKLns in all rour tests Por a single detercination., The Mezateriur Flask ani one of tus cortrols contedted a mola when sa.cples far fre otemcalnatlom rere taten, The rests of our tests are piven pelovw. Nitrogen. Phosphoric Acid. é mesentericus Vulgatis ---- .014 1.85 Prote®a Vulguris- —- — — — — — ~.01405 LE riprese. Liquefier- ----- — ,0154 J..70 mosaterdue - - -------- £0188 1,10 4 ata Utyricus - - -- ~---- - = .0238 0.380 bs An exanination of this table sivws a Seerease ai tos aterossn acd an iuisrease in tle Phospnoria ACTA, Vs iN 2 D wr Ratvricus is an excestion to This, wo 7 Wis may be accounted ror by tlie npnarent F-ebleness of tiie germ as Andaicated by cultures inade rom the rlask. Our .emts lave decreased rather than inereased the amount of Nitrogen, the enount of Phosphoric Acid *o0 More nal amount in tvo of tis rlasks. “Loated by the tests are pro abiy eve ae to the absence of orvanic ma terive., Tha Carm, Mvcolces, was not a inoewlation, It vould not he just to reine tin “rarernsor Stoklasats results ave meh pe Eko Le. IN som ayo tho Pheue se wh Ifo iaest re sconstantiv pov.e in mind the used was old ani liny, and, to ssy the Pom tte Our ASLA cohlivin paraliel those of “19 PmoFr es ould be ex eated, hn > GOs Laer had to work upon, We rogrot tarat toe toutl Vey iLt, Tt 34 a avallaple, so 2 Lone thier wo5008en winch groen hore ofr fii but nave Lneressed a * Fon double the origi- in— mi crect extent 1.0 -~_-* UG. .€t vallanle at the tiie of to tiat « .* YY. : y+ so obet ity royiye ty mea ple and even Sy ‘ ary in “wQ tapvoles + mom htte Least, wunvevor.pile in the Phosphoric or'sg as closely a tie .edla tie germs not Vor.’ ‘ oe 1 7 ee on at , - Orr ON e@ ee ~ N THAR 3 ER iii”