THE EFFECT OF VARYING THE TREATMENTS OF NITROGEN, PHOSPHORUS AND POTASSIUM ALONE AND IN' COMBINATION ON CERTAIN CHARACTERISTICS OF A DWARF GRAIN SORGHUM.’ By HENRY LANKFORD DUNTOIT A m s IS Subm itted to th e School of G raduate S tu d ie s o f M ichigan S ta te C ollege o f A g ric u ltu re and A pplied Science i n p a r t i a l f u lf i l l m e n t of th e requirem ents f o r th e degree of DOCTOR OF PHILOSOPHY Department o f S o il Science THE EFFECT OF VARYING THE TREATMENTS OF NITROGEN, PHOSPHORUS AND POTASSIUM ALONE AND IN COMBINATION ON CERTAIN CHARACTERISTICS .. OF A DWARF GRAEN SORGHUM. T h e s i s f o r t h e D e g r e e o f P h . D. MICHIGAN STATE COLLEGE H en ry L a n k fo r d D u n ton 1949 ACKNOY/LEDGEMENT The w r i t e r w ishes to ex p ress h i s a p p re c ia tio n to D r. G . E . M illa r and D r. L. M. Turk f o r th e v a lu a b le a s s is ta n c e and encouragement given th ro u g h o u t t h i s in v e s tig a tio n . The a u th o r i s a lso in d eb ted to D r. Boyd H arsh b arg er, V ir g in ia A g r ic u ltu r a l Experim ent S ta tio n f o r h i s h e lp in th e s t a t i s t i c a l a n a ly s is o f th e d a ta . TABLE OF CONTENTS Page I. INTRODUCTION 1 II. REVIEW OF LITERATURE 3 III. PURPOSE OF INVESTIGATION 4 IV. PLAN' OF EXPERIMENT. 5 A. L o catio n and D e s c rip tio n of A rea S elected 5 B. Chemical P r o p e rtie s o f th e S o il 6 C. F e r t i l i z e r Treatm ents and P lo t Design 6 D. Land P re p a ra tio n and Method of F e r t i l i z e r A ppli­ c a tio n S P la n tin g , C u ltiv a tin g and Thinning 9 E. V. RESULTS AND DISCUSSION A. . 10 The E f f e c t o f F e r t i l i z a t i o n on th e H eight of th e P la n t 10 B. Ih e E f f e c t o f F e r t i l i z a t i o n on S uckering, Heading and th e P e rcen t M oisture in th e Seed a t H arv est 13 C. The E f f e c t of F e r t i l i z a t i o n on th e Average h e ig h t o f Head, Weight o f Seed p e r Head, Weight o f Seed P e r Sucker Head, Weight of One Hundred Seed From R eg u lar and Sucker Heads, P e rc e n t G em in atio n and th e R e la tio n o f th e Weight of th e Head to th e Weight of th e Seed 19 D. Ih e E f f e c t of F e r t i l i z a t i o n on th e Browning of th e L eaves, Amount of D isease and Y ield 24 E. The E f f e c t o f F e r t i l i z a t i o n on th e N itro g e n , Phos­ phorus and P otassium C ontent of th e Seed 30 F. The E f f e c t o f F e r t i l i z a t i o n on th e F o lia g e C olor G. The E f f e c t o f F e r t i l i z a t i o n Seed. on th e C olor o f th e 36 39 TABLE OF CONTENTS - C o n tin u e d Page V I. SUMMARY AND CONCLUSIONS. .. 41 A. TheE f f e c t o f N . 41 B. TheE f f e c t o f P C. TheE f f e c t o f K D. TheE f f e c t o f Treatm ents on P la n t C h a ra c te r- ; i s t i c s (T able) 45 • '4 2 43 V II. FIC-URES SHOfTNG EFFECTOF TREATiaENTS ON PLANT . CKiuRACTSRISTICS V I I I . ; LITERATURE CITED 46 ~ 66 INTRODUCTION In g e n e ra l, th e g e n e tic com position of any p la n t s p e c ie s d e te r ­ mines i t s chem ical com position and p la n t c h a r a c te r is ti c s # However, th e chem ical co m p o sitio n -an d -p lan t c h a r a c t e r is t i c s may "be m odified t o a c e r t a i n e x te n t by v a r i a t io n s i n such environm ental f a c to r s as c lim a te a n d s o i l , e s p e c ia lly th e n u t r ie n t supply# Many in v e s tig a tio n s have shown th e e f f e c t o f a p p lic a tio n s o f n itro g e n , phosphdrus and p o tassiu m t o th e s o i l on p la n t c h a r a c t e r i s t i c s such as h e ig h t o f grow th, m a tu rity , c o lo r and com p o sitio n . These r e ­ v e a l th e f a c t t h a t v a r i a t io n s i n a p p lic a tio n o f n itr o g e n , phosphorus and p o tassium may a f f e c t th e p la n t growth and i n some cases cause v a r i a t i o n i n chem ical com position o f th e g r a in o r f r u i t . The chem ical com position o f th e le a v e s and stem s o f th e p la n t i s g e n e ra lly more r e ­ sponsive t o v a r ia tio n s in n u t r i e n t s th a n i s th e g rain # The seed o f th e p l a n t , re p re s e n tin g f u tu r e g e n e ra tio n s , i s i n g e n e ra l r e l a t iv e ly c o n s ta n t in m in era l com position# The acreag e o f dwarf g r a in sorghum i s in c r e a s in g in V ir g in ia on th o se farm s where th e r e i s a sh o rta g e o f la b o r . Less la b o r i s re q u ire d to produce an a c re o f g r a in sorghum th a n an a c re o f corn in V ir g in ia , because g r a in soreturn i s h a rv e s te d w ith a combine. G rain sorghum y ie ld s ap p ro x im ately n in e ty p e rc e n t o f adapted h y b rid corn u nder s im ila r c o n d itio n s . Hie replacem ent o f co rn by g r a in sorghum i s n o t ex p ected , b u t a f u r t h e r in c re a s e o f g r a in sorghum on th e w e ll mechanized farm w ith a la b o r sh o rta g e i s a n tic ip a te d # -2 No l i t e r a t u r e h a s been found r e p o rtin g th e e f f e c t o f v a ry in g th e r a t e o f a p p lic a tio n o f n itr o g e n , phosphorus and potassium upon th e ty p e o f growth and g r a in com position o f a dw arf g ra in sorgium i n th e e a s te r n U nited S ta te s * Rxe h e ig h t, m a tu rity , ty p e o f grow th, and y i e l d a r e v e ry im portant* S h o rte r o r dw arf grow th, e a r ly m a tu rity , heavy y ie ld and g r a in w ith a h i g i n u tr ie n t c o n te n t a re d esire d * Any e f f e c t o f f e r t i l i z a t i o n o f th e s o i l w ith n itro g e n , phosphorus and po tassiu m upon th e growth and development o f t h i s p la n t i s im portant* REVIEW OF LITERATURE Vandecaveye (19) h a s p re se n te d an e x c e lle n t review o f th e e f f e c t o f s o i l ty p e and f e r t i l i z e r tre a tm e n ts on th e chem ical com position o f c e r t a i n fo ra g e and sm all g r a in crops* The response o f c e r e a l g r a in s t o n itro g e n was found t o v a ry w ith th e amount a p p lie d t o th e s o i l and th e tim e o f a p p lic a tio n * In o nly seven o f f i f t e e n s tu d ie s reviewed d id th e a p p lic a tio n o f phosphorus in c r e a s e th e phosphorus c o n te n t o f th e g rain * P otash a p p lic a tio n s r e s u lte d in an in c re a s e o f potassium i n th e g ra in in on ly a few cases* Beeson (4) h as p re se n te d a review and co m p ilatio n on th e m in e ra l com position o f cro p s w ith p a r t i c u l a r re fe re n c e t o th e s o i l s i n which th e y were grown* s u lts * T h is review shows wide v a r i a t io n and c o n f lic tin g re ­ The heed f o r h a s ic re se a rc h on t h i s problem i s ev id en t* S tu d ie s by B a y field ( l ) (2) ( 3 ) , B l a i r ( 5 ) , Gainey, e t a l ( 8) , Geddes, e t a l ( 7 ) , Guyon ( 1 0 ), T e ak le, e t a l (17) and T ulaikov (18) in d ic a te t h a t a d d itio n s o f n itro g e n t o t h e s o i l r e s u lte d in an in c re a se d p e rc e n t o f p r o te in i n th e g rain * G arola ( 9 ) , H o ltz , e t a l ( 1 1 ), J o r e t , e t a l (12) and V in cen t, e t a l ( 20) re p o rt t h a t th e e f f e c t o f n itro g e n on th e p r o te in c o n te n t o f th e g ra in i s v a r ia b le , depending upon th e s o i l , tim e and amount o f a p p li­ c a tio n and a ls o o th e r fa c to rs * R eports o f Guyon ( 1 0 ), L e fo rt (14) and Murphy (15) show d e f i n i t e l y t h a t th e a d d itio n o f phosphorus f o r wheat in c re a se d th e p e rc e n t o f phos­ phorus in th e g rain * R e s u lts o f s tu d ie s by B ay fie ld ( l ) (2) ( 3 ) , -4- Geddes, e t a l ( 7 ) , V in cen t, e t a l (20) and Weidemann (22) in d ic a te t h a t th e phosphorus tre a tm e n t had l i t t l e o r no e f f e c t upon th e phos­ p horus c o n te n t o f th e g r a in . R e s u lts o f in v e s tig a tio n s d e a lin g w ith th e e f f e c t o f po tassiu m a p p lic a tio n s on th e p o tassiu m c o n te n t o f th e g r a in have been in c o n s is ­ te n t. Some i n v e s tig a to r s found t h a t p o tassiu m a p p lic a tio n s in c re a s e d t h e p o tassiu m c o n te n t i n th e g r a in w h ile o th e r s shoved t h a t po tassiu m had no e f f e c t i n in c r e a s in g th e p o tassium co n te n t o f th e g r a in . C a r t t e r ( 6) s t a t e s t h a t d i r e c t f e r t i l i z e r a p p lic a tio n s on a s o i l o f medium t o h ig h f e r t i l i t y shoved v e ry l i t t l e e f f e c t on th e y ie ld o r t h e com position o f soybean se e d . Weeks, e t a l (21) re p o rte d from Kentucky t h a t m anure, lim e and f e r t i l i z e r tre a tm e n ts a f f e c te d th e amount o f phosphorus, calcium , mag­ nesium and p o tassiu m and t o a l e s s e r e x te n t th e n itro g e n in t h e g ra in of com . PURPOSE OF INVESTIGATION The purpose o f t h i s i n v e s tig a tio n v a s t o d e te n n in e 9i f v a ry in g th e r a t e s o f n itr o g e n , p h osphorus, and p o tassium alone and i n com bination a p p lie d t o a p o o r s o i l v o u ld s ig n i f i c a n t l y in flu e n c e ( l ) th e n itro g e n , phosphorus and p o tassiu m c o n te n t o f th e sorghum g r a in produced on t h a t s o i l and ( 2) th e h e ig h t o f p l a n t , d a te o f head in g and p o ll i n a t i o n , m a tu rity , m o istu re p e rc e n t i n th e seed a t h a r v e s t, c o lo r o f le a v e s , c o lo r o f g r a in , number o f s u c k e rs , p e rc e n t g erm in atio n o f se e d , w eight p e r 100 g r a in s , p re v a le n c e o f d is e a s e and y i e l d . PLAN OF EXPERIMENT' L o catio n and D e s c rip tio n o f Area S ele c te d The experim ent was conducted on a G roseclose s i l t loam s o i l . The s o i l s o f th e G roseclose s e r i e s have developed from c h e rty d o lo m itic lim esto n e in a s s o c ia tio n w ith th e gray-brown p o d zo lic s o il s o f th e A ppalachian V a lle y . ac id in r e a c tio n . The G roseclose s o i l s a re medium to s tro n g ly The s i l t loams predom inate. The topography i s r o l l i n g to h i l l y . d ra in ag e a re good. I n te r n a l and" e x te r n a l The G ro seclo se s o il s o f th e s e c tio n a re p r a c t i c a l l y a l l c le a re d and used f o r a l l o f th e more common crops o f th e a re a . G roseclose i s found p r im a r ily in w estern V ir g in ia and e a s te rn Tennessee. „ The A h o riz o n i s l i g h t to y e llo w ish -g ra y in c o lo r and ranges from 7 to 9 in ch e s t h i c k . The B h o riz o n i s l i g h t brow nish-yellow f r i a b l e s i l t y c la y and i s 5 to 7 in c h e s t h i c k . The C h o riz o n i s s im ila r to th e B h o riz o n b u t“ sp lo tch ed w ith re d , brown and g ra y . m ents occu r l o c a l l y . A few sh a le f r a g ­ The C h o riz o n i s 18 to 36 in ch es th i c k . The ex p erim en tal a re a s e le c te d was lo c a te d on a 150 a c re p a s tu re f i e l d to which no f e r t i l i z e r had been ap p lied f o r 35 y e a r s . f e r t i l i z a t i o n a re n o t a v a ila b le beyond 35 y e a r s . Records o f Records show th e f i e l d had been in p a s tu re sin c e 1900 and o ld e r people in th e s e c tio n say t h a t th e a re a had been in p a s tu re co n tin u o u sly f o r over 74 y e a r s . ' One to n o f ground lim esto n e p e r acre was a p p lie d in 1940. The s o i l o f th e e n t i r e a re a was c a r e f u lly mapped and checked to determ ine th e b e s t lo c a tio n f o r th e experim ent. The fo u r acre a re a s e le c te d was n e a rly f l a t w ith enough slope to allow good su rfa c e drain ag e* The a r e a was th e n checked every fo u r o r f iv e f e e t t o d e t e r ­ mine th e depth o f th e to p s o i l , depth o f th e s u b - s o i l, ty p e o f s u b - s o il and c o lo r o f th e s o il* T h is was done t o lo c a te th e most uniform a r e a o f 3 /4 o f an a c re needed f o r th e experim ent. The a r e a used showed v ery l i t t l e v a r i a t io n i n d epth o f to p o r s u b - s o il, c o lo r o f s o i l and o th e r v is ib le c h a ra c te ris tic s • Chemical P r o p e r tie s o f th e S o il The a r e a was l a i d o u t in to p l o t s and from each p lo t a s o i l sample was taken* A quick s o i l t e s t a cco rd in g t o V irg in ia procedure was ru n on a l l th e sam ples. The s o i l s showed uniform ly low t o v e ry low i p a v a ila b le phosp h o ru s, u n ifo rm ly good in a v a ila b le p o tassiu m , good i n a v a ila b le c a l­ cium and magnesium, a pH o f 6*0 and an o rg a n ic m a tte r c o n te n t o f 1.3/£* A v a ila b le phosphorus was a ls o run on th e same s o i l samples by T ru o g 's method and a l l gave a low te s t * Base exchange p r o p e r tie s o f th e s o i l were determ ined by th e methods o u tlin e d by Pegeh, e t a l* (16)* The b ase exchange c a p a c ity was 9*38 m .e. p e r 100 grams o f d ry s o i l and t h e exchangeable Ca, Mg, K and H was 3 .5 9 , 1 .7 1 , 0*20 and 3*88 m.e* p e r 100 grams o f dry s o i l re s p e c tiv e ly * F e r t i l i z e r T reatm ents and P lo t D esign Four tre a tm e n ts o f n itr o g e n , two tre a tm e n ts o f p h osphoric a c id and two tre a tm e n ts o f p o ta sh were u sed i n a fo u r by two by two f a c t o r i a l design* Three r e p l ic a t io n s w ere used in s te a d o f fo u r due t o th e lack o f s u f f i c i e n t uniform land* were a s follow s* The tre a tm e n ts o f f e r t i l i z e r and p l o t d esig n -7 - — T reatm ents p e r a c re N itro g e n P hosphoric a c id 0 - 0 lb s , 1 - 60 l b s , 2 - 120 l b s . 3 - 180l b s . 0 P otash - 0 0 - 0 1 - 240 l b s , 1 - 120 l b s , P lo t D esign * R e p lic a te 1 . block b block a n: - p 2°5 " 0 3 3 - ■. 1 ■. 0 0 ' 2 m. , 1 0 2 « 1 1 m 0 1 0 m* , 1 . . k 20 1 0 0 0 1 1 0 1 N m 2 m 1 m 3 2 0 1 m 3 0 m *2°5 - k 2o 1 0 0 0 0 1 1 1 - 1 1 0 0 1 0 1 0 mr - R e p lic a te 2 . b lock a E ~ ■ , 2 0 2 1 1 0 3 3 1 0 0 0 - 1 •1 1 m 0 - . p2°5 block b " k 20 - - - 0 0 1 1 0 1 1 0 N 3 0 0 3 1 1 2 2 -?sP5 m m m m m 1 1 0 0 0 1 1 0 - k 20 0 0 1 1 0 1 1 0 R e p lic a te 3 . block a K 3 0 1 1 2 2 3 0 * mm mm 'm ■ .em - b lock b P2°5 - 1 1 0 - 0 1 0 0 k 2o 0 1:- ■ 1 0 0 1 1 0 • n; - P2°5 2 0 1 0 2 1 3 3 - 1 0 0 1 0 1 1 0 mm mm - — mm - k 20 0 1 0 0 1 1 1 0 Numbers r e f e r t o r a t e s p e r a c re in d ic a te d u nder "Treatm ent p e r a c r e " . The 240 l b . r a t e o f P20£ and th e 120 l b . r a te o f KjjO p e r a c re were th o u g h t t o be ample t o p re v e n t any d e f ic ie n c y from o c c u rrin g . The 180 l b . r a te o f n itro g e n was ex p ected t o be am ple. * The n itro g e n was su p p lie d a s 16$ n i t r a t e o f soda, th e phosphorus as 45$ t r i p l e superphosphate and th e p o tassiu m as 62$ m u riate o f p o ta s h . Land P r e p a ra tio n and Method o f F e r t i l i z e r A p p lic a tio n The sod lan d was plowed March 25 t o an average depth o f 7 to 8 in ch es w ith a t r a c t o r plow . tim e on May 1 3 . The lan d was double d isc e d A p ril 10 and a second The t r i p l e superphosphate and m u ria te o f p o ta sh were a p p lie d b ro a d c a st May 13 im m ediately b e fo re th e second d is c in g . F e rti­ l i z e r f o r each p l o t was mixed w ith s o i l b e fo re ap p ly in g t o p re v e n t d r i f t i n g o r blow ing. The f e r t i l i z e r and s o i l f o r each p l o t was d iv id e d in to f o u r eq u al p a r t s and each p a r t o r o n e -fo u rth was sown o v er th e e n t ir e p lo t to g iv e a more even d i s t r i b u t i o n and a s su re no s k ip s . On a l l p lo ts re c e iv in g 60, 120 and 180 l b s . of n itro g e n p e r a c re , 20 l b s . of th e n itro g e n was a p p lie d a t th e same tim e th e phosphate and p o ta s t were a p p lie d . Hie rem ainder o f th e n itro g e n o r th e 40, 100 and 160 l b s . was ap p lie d as a s id e d re s s in g on June 30 when th e p la n ts were about 12” high on th e b e t t e r p l o t s and 3” to 4" high on th e p o o re r p l o t s . Hie n itro g e n was a p p lie d in a band 12" to 18” vd.de between th e rows. P la n tin g , C u ltiv a tin g , and Thinning Hie p lo ts were 16.5* wide and 2 6 .5 ' long w ith f iv e 40" rows p e r p lo t Hie th r e e c e n te r rows were used f o r a l l m easurem ents. Hie sorghum was p la n te d th ic k w ith a se e d e r to in s u re a good stan d and was th in n e d to 4 in ch es between p la n ts in th e row on June 23. A m easuring s tic k was used in th e th in n in g , to in s u re equal d is ta n c e between p l a n t s . a lle y s between p l o t s . Hi ere were no Hie sorghum was c u ltiv a te d th re e tim e s, once w ith a r o ta ry hoe when 2" h ig h and tw ice w ith a r e g u la r c u l t i v a t o r . Plainsm an, s. dwarf g ra in sorghum v a r i e t y , was used which i s recom­ mended f o r V ir g in ia as a combine sorghum. Hie sorghum was p la n te d on May 15 which i s th e recommended p la n tin g d a te f o r th e a re a * in e x c e lle n t p h y s ic a l c o n d itio n when th e sorghum was p la n te d . m o istu re'w as p re s e n t th ro u g h o u t th e growing seaso n . Hie s o i l was Ample -1 0 RESULTS and DISCUSSION The E f f e c t o f F e r t i l i z a t i o n on th e H eight o f th e P la n ts The p la n t h e ig h t i s th e average o f t h r e e r e p lic a te s w ith 100 p la n ts measured from two rows o f each p lo t* a t o t a l o f 300 p la n t s were measured* J u ly 8 , August 5 and Septem ber 20* T h e re fo re , f o r each tre a tm e n t The p la n t h e ig h t was measured on The r e s u l t s a re shown in T able 1 and F ig u re 1 . The s t a t i s t i c a l a n a ly s is i s g iven i n T ables 2 and 3* Table 1 . - The average h e ig h t o f p l a n ts i n inches* Treatm ent * N - P205 0 1 — 2 3 0 1 — 2 — 3 0 -- ' 1 -2 3 m . 0 1 — 2 3 - * o 0 0 0 0 0 0 0 1 1 1 ^ ... 1 1 1 1 — — :. .— '- - r i - - J u ly 8 August 5 5.77 5.59 5.90 5 .9 0 7.23 6.24 6.44 6 .1 0 11.39 13.24 14.27 15.60 11.94 14.09 13.90 12.74 26.6 23.0 23.9 27.2 26.4 27.0 25.5 24.2 41 .4 4 5 .4 4 4 .8 4 4 .5 40.6 4 4 .7 42.9 4 4 .2 Septem ber 20 k 20 o 0 0 0 1 I 1 1 0 0 0 0 1 1 1 1 . See page 7 f o r tre a tm e n ts p e r acre* 28.0 24.0 25.0 25.7 26.5 26.0 25.9 26.0 4 2 .0 4 5 .1 4 5 .0 4 7 .1 _ 41 .0 4 6 .3 4 4 .0 43 .5 -11Table 2 Anal ys i s o f v a ria n c e o f th e h e ig h t o f th e p la n ts i n in ch e s on J u ly 8 * Source D.F. Blocks W F ■ K HP • HK HC HFK E rro r T o ta l S .S . 14.67 8.36 630.75 .19 17.62 9.85 4.08 2.77 36.69 724.98 3 1 1 3 1 3 27 47 M.S. 2.93 2.79 630.75**(+) .19 5.87* (+) 3.28 4.08 .92 1.36 ♦ S ig n if ic a n t a t 5% p o in t ♦ ♦ S ig n ific a n t a t 1% p o in t (+) in c r e a s e s (-) d e c r e a s e s Table 3 . - A n aly sis o f v a ria n c e o f th e h e ig h t of th e p la n ts i n in c h e s on August 5# Source Blocks H •P K HP HK PK HHC E rro r T o ta l D .F. . ' . . -• 5 3 1 1 3 3 1 3 27 47 S .S . : ♦ S ig n ific a n t a t 5% p o in t ♦ ♦ S ig n ific a n t a t 1% p o in t C+) in c r e a s e s ( - )d e c r e a s e s 93.50 13.50 3924.08 .33 54.09 17.17 6.76 16.81 165.76 4292.00 M.S. 18.70 4.50 3924.08**(+) .33 18.03 5.72 6.76 5.60 6.14 -1 2 - Phosphorus had more in flu e n c e on t h e h e ig h t o f p la n ts th a n n itro g e n o r p o ta ssiu m . grow th. Phosphorus was th e p r in c ip a l elem ent s tim u la tin g e a r ly N itro g en a lo n e , po tassiu m alone and n itro g e n -p o ta ssiu m combined had no s ig n i f i c a n t in flu e n c e on p la n t h e ig h t on J u ly 8 , b u t d ecreased s l i g h t l y th e h e i g i t on August 5 and September 20 compared t o th e check. P la n ts grown on n itro g e n -p h o sp h o ru s, and n itro g en -p h o sp h o ru s-p o tassiu m t r e a t e d p l o t s showed an in c r e a s e i n h e ig h t o v er th o se grown w ith phos­ phorus tre a tm e n t. P la n ts produced on NjP’jK]' f e r t i l i z e d p l o t s were t a l l e r th a n p la n ts from o r N3P^K^ p l o t s . P otassium alo n e and in com bination w ith n itro g e n and phosphorus had no s ig n i f i c a n t in flu e n c e on th e h e ig h t which was p ro b ab ly due to a s u f­ f i c i e n t supply o f po tassiu m i n th e 6o i l . s l i g h t l y when combined w ith NgP^ and NgP^. between n itro g e n and phosphorus o c c u rre d . P otassium d ecreased th e h e ig h t A s ig n i f i c a n t in t e r a c t io n P la n ts grown on n itro g e n - phosphorus t r e a t e d p l o t s were t a l l e r th a n p la n ts on phosphorus t r e a te d p l o t s th ro u g h o u t th e growing se a so n . N itro g en and phosphorus were both needed to g iv e p la n ts h e ig h t, w ith phosphorus h aving th e g r e a te s t in ­ flu e n c e . Of i n t e r e s t i s th e f a c t t h a t n itro g e n had much le s s in flu e n c e on th e h e ig h t th a n p hosphorus. -1 3 - The E f f e c t o f F e r t i l i z a t i o n on S uckering, H eading, M a tu rity , P o llin a tio n and th e P e rc e n t M oisture i n th e Seed a t H arvest The average number o f su ck e rs p e r 15 f o o t row was determ ined September 25, f iv e days b e fo re h a r v e s tin g . The numbers a re averages f o r th e th r e e r e p l ic a t e s and th e th r e e c e n te r rows o f each p l o t . Heading d a ta were ta k e n from th e same p la n ts a s th e su ck erin g d a ta . These d a ta a re p re se n te d i n Table 4 and F ig u re s 2, 3, 4 , and 5 , a n a ly s is i s p re se n te d in T ables 5, 6, 7 , and 8 , Table 4 , - — - 0 0 0 0 0 0 d 0 1 1 1 1 1 1 1 mm - S uckering and M a tu rity R ate as In flu en ced by F e r t i l i z a t i o n , T reatm ent* N -P 2O5- K2O 0 1 2 3 0 1 2 3 0 1 2 3 0 1 2 3 The s t a t i s t i c a l 0 0 0 0 i -■ 1 mm 1 - 1 - 0 - 0 0 mm 0 mm 1 1 - 1 1 Suckers I P e rc e n t P e rc e n t p la n ts brown per 15 f t , f u l l head heads row August 5 August 20 2.22 6,74 7 .9 1 7.97 5.70 4 .2 5 6.31 3 .4 2 11.61 16.06 25.10 13.49 10.00 32.45 37.45 24.70 6 .1 1 .8 1 .7 3 .5 8 .6 4 .6 3 .4 1 .4 6 9.2 85.0 83.3 84.9 6 4 .2 86.5 85.3 8 7 .0 0 0 0 0 0 0 0 0 80.0 95.3 98.0 97.0 92.7 95.3 9 3.7 97.3 * See page 7 f o r tre a tm e n ts p e r a c r e . P ercen t suckers p o llin ­ a tin g August 20 P e rc e n t m oisture i n seed S e p t. 30 _ .62 40.0 44.6 43.1 39.1 42.4 38.2 39.6 41.4 29.3 27.5 29.0 23.2 26.0 25.7 24.4 28.7 .39 _ 3.8 .36 66.2 84.7 88.9 88.1 50.1 83.2 84.3 91.5 . P e rc e n t m o istu re in su ck er seed S e p t. 30 4 4 .5 59.4 60.9 62.0 4 9.7 63.3 68.0 38.1 35.9 41.6 33.9 3 7 .7 35.3 34.0 4 1.5 4 2 .0 Table 5 . - A n a ly sis o f V ariance o f th e Humber o f Suckers p e r F if te e n F eet o f Row. Source Blocks H P K HP HK PK HPK E rro r T o ta l D .F. 5 3 1 1 3 V 3 1 3 27 47 . , S .S . :7.7795 13.3245 54.8269 1.6060 4.1499 .2625 4.1419 3.6309 26.9999 116.7220 M.S.— 1.5559 4.4415 *(+) 54.8269**(r+-) 1.6060 1.3833 .0875 4.1419 1.2103 1.0000 ♦ S ig n if ic a n t a t 5% p o in t ♦ ♦ S ig n ific a n t a t 1% p o in t (-+•) in c r e a s e s (-)d e c re a se s Table 6 #- A n aly sis o f V ariance of th e P e rc e n t P la n ts i n F u ll Head August 5 . Source Blocks H P K HP HK PK HPK E rro r T o ta l ♦ S ig n ific a n t a t 5% p o in t ♦ ♦ S ig n ific a n t a t 1^ p o in t C+) in c r e a s e s ( - ) d e c re a ses D .F. 5 3 1 1 3 3 . 1 3 27 47 S .S . 11.9507 97.3667 34267.3313 12.5359 326.4915 35.3671 5.1025 38.3138 260.4227 35554.8822 M.S. 2.3901 32.4556 * ( " ) 34267.3313**(4-) 12.5359 275.4972** (-f-) 11.7890 5.1025 12.7713 ~ 9.6453 -1 5 - Table 7 . - A n aly sis o f V ariance o f th e P e rc e n t o f th e Suckers P o llin a tin g on August 20. Source D .F. 5 3 1 1 3 3 1 3 27 47 Blocks ST P K BP HK PK HPK E rro r T o ta l M.S. S .S . 74.7080 107.7226 45896.4114**(+) 3.8307 549.7949 *(+} " 8.4587 60.7951 59.8492 141.5952 373*5402 323.1680 45896.4114 3.8307 1649.3847 25.3762 60.7951 179.5476 3823.0717 52155.5780 ♦ S ig n if ic a n t a t 5$ p o in t ♦ ♦ S ig n ific a n t a t if* p o in t ( ^ in c re a s e s (-)d e c re a .se s Table 8 «- A n aly sis o f V ariance o f t h e P e rc e n t M oisture in th e Seed^ When Cut September 30# Source Blocks N P K HP HK PK HPK E rro r T o ta l D .F. ' ♦ S ig n ific a n t a t 5}£ p o in t ♦ ♦ S ig n ific a n t a t 1% p o in t (+ ) in c r e a s e s (- )d e c r e a s e s 5 3 1 1 3 3 1 3 27 47 M.S.' S .S . 13.3648 4.4940 914.7293 6.1776 1.0571 49.9242 .0332 12.6901 44.2049 1046,6753 - 2.6730 1.4980 914.7293 * * (-) 6.1776 .3524 16.6414 *(+) .0332 4.2300 1.6372 -1 6 - Suckering* N itro g e n alo n e and phosphorus alone in c re a se d s i g n i f i ­ c a n tly th e amount o f su ck e rin g compared to no tre a tm e n t* N itro g en -p h o s- phorus in c re a se d th e su c k e rin g above phosphorus alo n e and n itro g e n p h ospherus-potassium in c re a s e d su ck erin g above nitrogen-phosphorus* P la n ts on p l o t s t r e a t e d w ith NgPx orN gPjK x showed an in c re a s e i n su c k erin g above p la n ts grown on p l o ts to which Njp-j/ o r HjPjK-l was a p p lie d ; b ut p la n ts on p l o t s re c e iv in g NgF^ and th a n p la n ts on p l o t s re c e iv in g N ^ i o r W s u c k e r e d le s s l* I t i s i n t e r e s t i n g t o n o te t h a t a ls o th e y ie ld was low er on NgP-^ and NgF-jKi t r e a t e d p l o t s th a n NgP-^ o r NgP-jKx f e r t i l i z e d p lo ts * Corn and /_ b a rle y y ie ld s in V ir g in ia have shown s im ila r r e s u l t s i n c e r ta in e x p e ri­ ments* S uckering i s in c re a se d p r im a r ily by phosphorus and n itro g e n * There were no s ig n i f i c a n t in t e r a c t io n s w ith NP, NK, PK o r NFK* H eading and F e l l i n a t i n g : The e a r l in e s s o f h ead in g and p o l l i n a tio n showed a h ig h ly s ig n i f i c a n t in c re a s e from th e phosphorus tre atm e n t* N itro g en alo n e d ecreased s i g n i f i c a n t l y th e e a r lin e s s o f heading* N itro - gen-phosphorus in c re a se d s i g n i f i c a n t l y th e e a r lin e s s compared to phos­ phorus f e r t i l i z a t i o n alone* p o l l i n a t i o n and heading* P otassium had no e f f e c t on th e d a te o f In cre a se d r a t e s o f n itro g e n above 60 pounds p e r a c re had no in flu e n c e on t h e d a te s o f h ead in g o r p o llin a tio n * Of i n t e r e s t i s th e f a c t t h a t th e sorghum on p l o t s f e r t i l i z e d w ith n itro g e n phosphorus headed and p o llin a te d e a r l i e r th a n p la n ts grown on phosphorus f e rtiliz e d p lo ts • T h is f a c t showed t h a t n itro g e n d id n o t d e la y m a tu rity -1 7 - i f p ro p e rly balanced w ith phosphorus and p o tassiu m . There was a s ig n i f i c a n t i n t e r a c t io n between n itro g e n and phosphorus. M a tu rity and M oisture p e rc e n t i n th e G rain: The g ra in was h a r ­ v e ste d on September 30 which was f iv e days a f t e r th e f i r s t k i l l i n g fro s t. The g r a in co n tain ed to o much m o istu re to th r a s h w ith a r e g u la r head t h r a s h e r . E ig h t h eads from each p l o t were th ra sh e d by hand and th e m o istu re p e rc e n t d eterm in ed . G rain produced on p lo ts t r e a t e d w ith n itr o g e n alo n e and p o tassiu m alone co n tain ed approxim ately th e same p e rc e n t m o istu re as th e g r a in on no tre a tm e n t p l o t s . G rain from phos­ phorus t r e a t e d p l o t s co n tain ed t e n p e rc e n t l e s s m o istu re th a n seed from th e check p l o t which was s i g n i f i c a n t . Seed from n itro g en -p h o sp h o ru s t r e a t e d p l o t s showed a low er m o istu re c o n te n t th a n seed from th e phos­ phorus tre a tm e n t a lo n e . There i s an in d ic a tio n t h a t potassium w ith phosphorus o r p otassium w ith n itro g en -p h o sp h o ru s gave g ra in w ith a s l i g h t l y d ecreased m o istu re c o n te n t. In c re a se d r a t e s o f n itro g e n d id n o t in flu e n c e s i g n i f i c a n t l y th e p e rc e n t m o istu re i n th e g ra in a t h a r ­ v e s t. N itro g e n -p o tassiu m t r e a t e d p l o t s developed g r a in w ith a s i g n i f i ­ c a n tly h i ^ i e r m o istu re c o n te n t a t h a rv e s t th a n g ra in from p l o t s f e r t i l i z e d w ith n itro g e n o r po tassiu m a lo n e . F e r t i l i z a t i o n shows th e same e f f e c t on m a tu rity a s on head in g and p e rc e n t m o istu re i n seed a t h a r v e s t. N itro g en h a s te n s m a tu rity when b alan ced w ith phosphorus on a s o i l w e ll su p p lied w ith p o ta s h . D uring 1947 and 1948 th e s e r e s u l t s were d u p lic a te d w ith co rn on exp erim en tal p l o t 8 i n V ir g in ia . -1 8 - Suckers* The su ck ers headed, p o llin a te d and m atured ap p ro x im ately two weeks l a t e r th a n th e r e g u la r heads* The in flu e n c e o f f e r t i l i z a t i o n on th e s e c h a r a c t e r i s t i c s i s n e a r ly th e same as f o r th e r e g u la r heads b u t i s n o t as c o n s is te n t* -1 9 - The E f f e c t o f F e r t i l i z a t i o n on th e Average W eight o f Head, W eight o f Seed P e r H ead,; W eight of Seed P e r Sucker Head, Y/eight o f 100 Seed from R eg u lar and Sucker H eads, P e rc e n t G erm ination o f Seed A f te r Dry (12$ m o istu re) and th e R e la tio n of th e Weight o f th e Head t o th e Weight o f th e Seed* A ll measurements were made w ith seed a t 12$ m oisture# The r e ­ s u l t s a re given i n Table 9 and F ig u re s 6, 7 , 8 , 9 , 10, 11, and 12* S t a t i s t i c a l a n a ly se s o f th e measurements are given in Tables 10, 11, 12, and 13# Table 9#- The E f f e c t o f F e r t i l i z a t i o n on th e Heads, Seed and Germ ination# T reatm ent * W t.P er Wt.Seed per W -P 205- k 2o Head Grains Head Grams Q1 2 3 o; l 2 3 0 1 2 3 0 - 1 2 3 — * 0 0 0 0 0 0 0 0 1 1 1 x 1 1 mm mm - 0 0 0 0 1 1 1 1 0 0 0 0 1 1 1 1 See page 7 4 8 .5 4 1 .2 3 7.1 4 1 .7 4 6 .2 41 #2 4 4 .4 3 5 .8 68.9 6 4 .8 8 1 .1 75.2 6 1 .2 7 2 .0 6 7 .0 7 3.4 35.8 24,0 26.3 33.1 3 9.0 34.9 3 6 .2 27.2 4 7.6 52.5 62.5 5 6 .2 4 8 .5 4 8 .5 4 7 .6 5 6 .2 Wt.Seed w t.head p e rc e n t 81.9 81.9 8 1 .0 83.3 81.9 82.3 8 1 .4 8 2 .2 83.3 8 1.8 8 2 .0 80.5 8 1 .2 8 1 .2 8 2.3 8 1 .4 f o r tre a tm e n ts p e r acre# W t.seed per sucker head Grams Wt.100 Seed Grams 11.8 10.4 9 .1 16.8 16.3 16 .8 15.6 13.1 16.3 23.6 24.5 26.7 23.1 26.3 22.2 21.7 2.40 2.08 2.22 2.16 2.42 2.34 2.30 2.10 2S.58 2.69 2.85 2.74 2.62 2.66 2.72 2.80 Wt.100 Germin­ seed a tio n from p e rc e n t suckers grams 1.98 1.79 1.72 ,1.74 1.94 1.73 1.88 2.01 2.27 2.25 2.57 2.41 2.09 2.41 2.41 2.44 87.9 83.3 79.6 88.1 86.9 88.0 8 7 .2 92.3 94.0 94.9 91.9 96.3 9 5.1 93.3 91.8 9 1.7 -2 0 Table 1 0 .- A n aly sis o f V arian cs o f th e Weight P e r Head a t 12$ M o istu re. D.F. Source 5 3 1 1 3 3 1 3 27 47 Blocks H P K HP HK FK HPK E rro r T o ta l S .S . .0017 .0002 •0469 .0003 •0028 .0006 .0002 .0014 .0046 .0587 _ M.S. .00033 .00007 .04688**(+) .00028 .00094 *(+ ) .00021 .00023 .00047 .00017 > ♦ S ig n if ic a n t a t 5$ p o in t ♦ ♦ S ig n ific a n t a t 1$ p o in t (rf-) in c r e a s e s ( - ) d e c re a se s Table 1 1 .- A n aly sis o f V ariance o f th e W eight o f th e Seed P e r Head a t 12$ M o istu re . Source Block8 E P K HP HK PK HPK E rro r t T o ta l * ♦ S ig n ific a n t a t 5$ p o in t ♦ ♦ S ig n ific a n t a t 5$ p o in t (-(^ in creases (- ) d s c r e a s e s D .F. S .S . 5 3 1 1 3 3 1 3 27 47 .000387 .000429 .024843 .000004 .002191 .000398 .001046 .001529 .003617 •034444 M.S. .000077 .000143 .024843**(+) .000004 .000730 *(+) .000133 .001046 *(+) .000510 * ( - ) .000134 21T able 1 2 .- A n a ly s is o f V a ria n ce of th e P e rc e n t o f Head W eight I s S e e d s. Source D .F . B locks H P K HP HK PK HFK E rro r T o ta l 5 3 S .S . M .S. 23.60 1 .5 2 4 .7 2 .5 1 1 .8 8 .8 8 1 .0 5 .0 5 2.2 3 1 .4 4 .0 7 4 .6 6 3 .5 5 3 3 1 3 27 47 6 .6 8 4 .3 1 .0 7 13.99 9 5 .6 2 14 6 .7 5 ■ ^S ig n ifican t a t 5% p o in t ^ S i g n i f i c a n t a t 1% p o in t (+ )in c r e a s e s ( - ) d e c r e a s e s T able 1 3 .- A n a ly s is o f V arian ce o f t h e W eight o f 100 Sorghum K e rn e ls . Source B locks K P K HP M PK HPK E rro r T o ta l . ■ ^ S ig n if ic a n t a t 5 fa p o i n t * * S i g n i f i c a n t a t l/£ p o i n t (-f)in c r e a s e s (-)d e c r e a se s D .F . 5 3 1 1 3 3 1 3 27 47 S .S . .057231 .062115 2.485666 .010414 .333069 .035115 .021973 .055322 .353912 3.414817 M.S. .011446 .020705 2.485666**(+) .010414 .111023 *(+ ) .011705 .021973 .018441 .013108 - 22- Table 1 4 , - A n aly sis o f V ariance o f th e P erc e n t G erm ination o f th e Seed, Source : D .F, B locks N ‘ P K UP HK PK HPK E rro r T o ta l ♦ S ig n ific a n t a t 5% p o in t ♦ ♦ S ig n ific a n t a t 1% p o in t (+) in c r e a s e s ( - ) d e c rea se s 5 3 1 1 3 3 1 3 27 47 S .S . 92,7949 116.1318 545.4008 7.8085 26.9593 22.3159 70.4222 37.9469 533.3847 1453.1650 M.S., 1.8559 38.7106 545.4008**(+) 7.8085 8.9864 7.4386 70.4222 12.6490 19.7550 23Weigfrt P e r Heads Heads produced on p l o t s t r e a t e d w ith n itro g e n alo n e were l i g h t e r th a n th o s e from th e u n f e r t i l i z e d p lo t* Heads from n itro g e n -p o ta s siu ra t r e a t e d p lo ts were s l i g h t l y l i g i t e r th a n h eads from p l o t 8 f e r t i l i s e d w ith p o tassiu m alo n e o r th e cheek. n itr o g e n alo n e had no in flu e n c e * In c re a se d r a te s o f Heads from phosphorus t r e a t e d p l o t s were s i g n i f i c a n t l y h e a v ie r th a n th o s e from no tre atm e n t* The n itro g e n - phosphorus t r e a t e d p l o t s produced h ead s s i g n i f i c a n t l y h e a v ie r th a n th o se grown on p l o t s t r e a t e d w ith phosphorus alone* Hie n e c e s s ity o f h aving an adequate supply o f n itro g e n and phosphorus to produce th e h e a v ie s t head was ev id e n t even though phosphorus had th e g r e a te s t in flu e n c e on th e w eig h t o f th e head* Phosphorus and n itro g en -p h o sp h o ru s had a s ig n i­ f ic a n t in flu e n c e on th e w eight p e r head* Die w eight o f seed p e r h ead , w eight o f seed p e r su ck er h ead , w eight o f 100 seed from r e g u la r h ead s and w eight o f 100 seed from su ck er h ead s showed n e a r ly th e same e f f e c t from f e r t i l i z a t i o n a s d id th e w e ig it p e r head* F e r t i l i z a t i o n had no in flu e n c e on th e r e l a t i o n o f th e w eight o f h eads t o t h e w eight o f seed s p e r head* G erm inations Die p e rc e n t g e rm in a tio n was determ ined by germ in atin g th e seed i n a sta n d a rd germ ination* Die e f f e c t o f v a rio u s tre a tm e n ts on t h e g erm in a tio n does n o t p re s e n t a tr e n d from which c o n c lu sio n s may h e drawn f o r a l l tre a tm e n ts* Die average p e rc e n t g erm in a tio n o f th e seed from a l l p l o t s f e r t i l i z e d w ith phosphorus i s 93*63/° and from a l l p l o t s w ith o u t phosphorus f e r t i l i z a t i o n i s 8 6 *6 6 Seed produced on phosphorus t r e a t e d p l o t s showed a s i g n i f i ­ c an t in c re a s e i n th e p e rc e n t g erm in a tio n compared t o seed produced on -2 4 - p l o t s re c e iv in g no phosphorus* Seed from phosphorus t r e a t e d p l o t s gave a q u ick er g erm in atio n th a n seed from p l o t s re c e iv in g no phosphorus f e r t i l i s a t i o n * I n Table 18 i t i s shown t h a t seed from phosphorus t r e a t e d p l o t s co n tain ed s ig n i f i c a n t l y mors P2O5 and i n Table 1 i t i s shown t h a t phosphorus in ­ c re a s e s th e e a r ly growth* Thus i t i s seen t h a t phosphorus p la y s a v i t a l r o le from g erm in atio n t o m atu rity * The E f f e c t o f F e r t i l i z a t i o n on th e P e rc e n t o f th e Leaves Browning and D rying on September 10, P rev alen ce o f D isease and Y ield i n B ushels P e r : A cre, Counts were made o f th e number o f le a v e s browning on September 10* Ore t o t a l number o f le a v e s browning in two f i f t e e n f o o t rows o f each p l o t were counted and t h e p e rc e n t determ ined* The number of d ise a se d p la n ts were counted i n two f i f t e e n f o o t rows o f each p l o t and th e t o t a l number o f d ise a se d p la n ts i n 30 f e e t o f row was recorded* The y ie ld was d e te r ­ mined by h a r v e s tin g two f i f t e e n fo o t rows from each p l o t , d ry in g th e h eads and seed t o 12/£ m o istu re and th e n th ra s h in g and weighing* were c a lc u la te d i n b u sh e ls (56 lb s* ) p e r acre* The y ie ld s The r e s u l t s o f th e s e d e te rm in a tio n s a r e p re se n te d i n Table 15 and F ig u re s 13, 14, and 15* S t a t i s t i c a l a n a ly s is o f th e d e te rm in a tio n s i s p re se n te d i n T ables 16 and 17. -2 5 - T able 1 5 .- She E f f e c t o f F e r t i l i z a t i o n on th e Browning o f th e L eaves, Amount o f D isease and Y ie ld . T reatm ent * . . . . . . . . . . . 0 1 2 3 0 1 2 3 6 1 2 3 0 1 2 3 * - 0 - 0 - 0 - 0 - 0 - 0 - 0 - 0 - 0 - 1 - 0 - 1 - 0 - 1 - 0 - 1 - 1 - 0 - 1 - 0 - 1 - 0 - 1 - 0 - 1 - 1 - 1 - 1 - 1 - 1 - 1 - 1 Brown le a v e s S e p t. 10 p e rc e n t Y ield-B ushels Humber D iseased P e r Acre P la n ts - 30* Row 12. 0£ m o istu re S e p t. 10 16.0 13.0 10.0 6.0 10.0 10.0 10.0 10.0 36.0 20.0 10.0 10.0 37.0 16.0 10.0 6.0 See page 7 f o r tre a tm e n ts p e r a c r e . .9 7 .65 .75 .64 .63 1.11 .67 .78 1.64 2.88 2.41 2.89 2.30 2.60 3.76 2.16 4 5 .2 27.2 27.2 35.8 40.3 4 4 .7 42.3 33.2 55.5 65.4 89.9 8 0 .2 56.2 8 2.5 72.3 ' 7 2.5 26- Table- ,16.~, . A n a ly s is o f V a ria n ce o f t h e Number o f D isea sed P la n ts P e r 30 F e e t o f Row* Source D .F . B locks 5 3 F 1 . NP m PK NPK E rro r T o ta l 1 3 - 3 K■ ■ 1 3 27 47 . S .S . M.S. v 9.8380 1.8773 39.1324 .2611 2.1735 1.3239 .1323 2.0820 16.3476 73.1681 1.9676 .6258 39.1324**(+ ) .2611 .7245 .4413 .1323 .6940 .6055 ♦ S ig n if ic a n t a t 5f0 p o in t ♦ ♦ S ig n if ic a n t a t 1% p o in t ( + ) in c r e a s e s ( - ) d e c re a s e s T ab le 1 7 .- A n a ly s is o f V a ria n ce o f t h e Y ie ld i n B u sh e ls P e r Acre a t 12^ M o istu re* Source d .f B locks P ■K- HP NK PK NPK E rro r T o ta l 5 3 ~ ? ,-y. • . " S .S . ■: 1 1 3 3 1 ; 3 27 - 47 ♦ S i g n i f i c a n t a t 5% p o i n t ♦ ♦ S i g n i f i c a n t a t 1% p o i n t (+ ) in c r e a s e s ( - ) d ecreases ■ 193.39 4 8 0 .3 1 14560.33 58.08 1988.42 1 930.94 1 99.27 4 3 2 .1 0 188.66 19024.50 M .S. 38.68-M?1 6 0 .1 0 * * (-) 14560.33**(+ ) 5 8 .0 8 * * (-) 662.81**(+ ) 3 1 0 .3 1 * * (-) 1 9 9 .2 7 * * (-) 1 4 4 .0 3 * 4 - ) 6.7 3 27- Browning o f leav esx Sorghum produced on p l o t s f e r t i l i s e d w ith n itro g e n alo n e showed l e s s browning th a n t h a t produced on th e check* A d e c re a se o f browning g e n e r a lly o ccu rred a s th e r a te o f n itro g e n was i n ­ creased* The amount o f browning of th e le a v e s was sm all on p la n ts from a l l p l o t s ex cep t w ith phosphorus alo n e and w ith phosphorus-potassium tre a tm e n ts * The p la n ts on th e s e two p l o t s showed a h ig h p e rc e n t of browned leav es* The p l a n t s on th e u n f e r t i l i s e d p lo t showed much le s s browning th a n th e p la n ts on th e phosphorus alo n e o r phosp h o ru s-p o tas­ sium t r e a t e d p lo ts * T h is i s t o be expected sin c e th e check p l o t re ­ ceiv ed no phosphorus to s tim u la te growth and encourage th e development o f n itro g e n d e ficien c y * The p e rc e n t o f le a v e s browning Showed t h a t 60 pounds o f n itro g e n w ith phosphorue o r w ith phosphorus-potassium was n o t s u f f i c i e n t t o p rev e n t browning b u t t h a t p la n ts on p l o t s t r e a t e d w ith 120 pounds o f n itro g e n showed no in c re a s e i n browning compared t o le a v e s from p la n ts produced on p l o t s re c e iv in g 180 pounds o f n itro g e n * T issu e t e s t s (Purdue method) f o r n itr o g e n , phosphorus and p o tassium were made on p la n ts from a l l p l o t s on J u ly 24, August 20 and Septem ber 20* P la n ts on a l l p l o t s showed h ig h i n n itro g e n and po tassiu m on J u ly 24* P la n ts on p l o t s re c e iv in g no phosphorus showed v e ry low i n phosphorus; b u t p la n ts on phosphorus f e r t i l i z e d p l o t s showed high* On August 20 p la n ts on a l l p l o t s showed high in potassium * t e s t s f o r phosphorus gave th e same r e s u l t s as on J u ly 24* T issue P la n ts grown on p l o t 8 re c e iv in g phosphorus and phosphorus-potassium f e r t i l i z a t i o n gave & low t e s t f o r n itro g e n * medium t o h ig h in n itro g e n * P la n ts from a l l th e o th e r p l o t s showed -28« The Septem ber 10*t i s s u e t e s t s p r e s e n te d a changed p i c t u r e f o r n itro g e n # The p l a n t s from t h e p l o t r e c e iv in g no f e r t i l i z e r gave a medium t e s t f o r n itr o g e n # a ls o from N-jK^, n itr o g e n # P-jKi P la n ts from N2 » and K3 tr e a tm e n ts and and NgK-^ tr e a tm e n ts showed h ig h t o v e ry h i ^ i i n The t i s s u e t e s t showed no n itr o g e n in th e p l a n t s from f e r t i l i z e d p lo ts # n itr o g e n b u t p l a n t s from P la n ts from th e and tr e a tm e n ts t e s t e d low i n tr e a tm e n ts showed h ig h # P la n ts from phosp h o ru s t r e a t e d p l o t s s t i l l t e s t e d h ig h b u t p l a n t s on p l o t s r e c e iv in g no p hosphorus showed none i n t h e p l a n t . T h is t e s t i n d i c a t e s t h a t p hosphorus was v e ry d e f i c i e n t i n t h i s s o i l ; b u t t h a t th e r a t e o f 240 l b s . o f F 2 O5 p e r a c r e was ample t o p re v e n t any d e f ic ie n c y o f phosphorus# P la n ts from p l o t s n o t r e c e iv in g p h o sp h o ru s showed phos­ p h o ru s d e f ic ie n c y symptoms; b u t no such symptoms w ere found on p l a n t s produced on phosphorus t r e a t e d p lo ts # On Septem ber 10, p l a n t s from a l l p l o t s showed h ig h i n p o ta ss iu m . The t e s t shows t h a t ample p o ta ss iu m was p r e s e n t i n t h e s o i l b e fo re th e 120 1b . a p p lic a tio n o f KgO was made# The t i s s u e t e s t s f o r n itr o g e n c o r r e l a t e q u ite c lo s e ly w ith th e p e r c e n t brown le a v e s on Septem ber 10# D ise a se s P la n ts w ere o b serv ed a t m a tu r ity which b ro k e a t th e ground l e v e l and had a d ry s t a l k . The p l a n t p a th o l o g i s t examined t h e s e s t a l k s and d ia g n o se d t h e d is e a s e a s a h th ra c n o s e . W ith th e h e lp o f t h e p l a n t p a t h o l o g i s t , c o u n ts f o r d is e a s e d p l a n t s w ere made on a l l p l o t s Septem ber 10# The r e s u l t s o f t h e c o u n ts a re d i f f i c u l t t o i n t e r p r e t due t o t h e i r in c o n s is te n c y , how ever, s i g n i f i c a n t l y more d is e a s e d p l a n t s w ere found on p l o t s t r e a t e d w ith phosphorus# No re a so n can be g iv e n f o r -2 9 - i h i s e x ce p t t h a t sorghum on p l o t s r e c e iv in g p h o sp h o ru s was more v ig o ro u s and p ro b a b ly more s u c c u le n t and th u s more s u s c e p tib le t o th e d is e a s e . Work by p la n t p a t h o l o g i s t s i n V ir g i n i a , h ow ever, shov/s t h a t g e n e r a lly th e more v ig o ro u s p l a n t s on w e ll f e r t i l i z e d s o i l a r e more r e ­ s i s t a n t to d is e a s e r a t h e r th a n more s u s c e p tib le a s shown h e r e . Y ie ld s e s tin g . The e f f e c t o f t h e tr e a tm e n ts on t h e y i e l d i s q u ite i n t e r ­ The f a c t t h a t a 45 b u s h e l y i e l d was se c u re d on th e check p l o t i s u n u s u a l. N itro g e n a lo n e d e c re a se d t h e y i e l d s i g n i f i c a n t l y compared t o no f e r t i l i z e r . P la n ts ho n itr o g e n a lo n e p l o t s d id n o t d e v elo p a s good h e a d s a s th o s e on t h e check p l o t s . A p p a re n tly , th e u n b alan ced n u t r i e n t c o n d itio n due t o t h e e x ce ss n itr o g e n had a d e tr im e n ta l e f f e c t on seed fo rm a tio n . The n itr o g e n may h av e s tim u la te d fo ra g e grow th which u sed up t h e a v a i l a b l e pho sp h o ru s r e s u l t i n g i n a d e f ic ie n c y o f a v a il a b le p hosphorus f o r seed fo rm a tio n . P la n ts from p l o t s f e r t i l i z e d w ith p o ta ssiu m a lo n e and n itr o g e n p o ta ssiu m d e c re a se d th e y i e l d s i g n i f i c a n t l y below th e y i e l d s from th e no tr e a tm e n t p l o t s . T h is i s p o s s ib ly due t o an u n b a lan c e d n u t r i e n t su p p ly w ith a d e f ic ie n c y o f pho?> h o r u s .' P la n ts grown on phosp h o ru s t r e a t e d p l o t s gave s i g n i f i c a n t l y h ig h e r y i e l d s th a n th o s e grown on t h e c h ec k , n i t r o g e n , p o ta ss iu m o r n i tr o g e n p o ta ssiu m t r e a t m e n t s . T h is shows two th i n g s : ( l ) th e d e f ic ie n c y o f phosp h o ru s i n t h i s s o i l and ( 2 ) th e n e c e s s i t y o f p h o sp h o ru s f o r seed p r o d u c tio n . N itro g e n -p h o sp h o ru s tr e a tm e n ts gave s i g n i f i c a n t in c r e a s e s i n th e y i e l d above phosp h o ru s a lo n e which a ls o d e m o n stra te s t h e im p o rt­ ance o f n itr o g e n i n seed p ro d u c tio n when ample p h o sp h o ru s i s p r e s e n t . . P la n ts produced on t h e N'jP^ f e r t i l i z e d p l o t s gave a lo w e r y i e l d -3 0 - th a n th e p l a n t s from th e NgPi t r e a t e d p l o t s . However t h e p l a n t s on t h e N2 P i p l o t s gave a h ig h e r y i e l d th a n th e p l a n ts on t h e p lo ts . Of i n t e r e s t i s t h e - f a c t t h a t t h e h ig h e s t y i e l d from p l o t s t r e a t e d w ith NPK came from t h e tr e a tm e n t and n o t from th e E g P ^ i o r ^ 3 ^ 1 ^ l t r e a tm e n ts . The reduced y i e l d from p l o t s tre a te c h -w ith NsPjj NgPiKi and NgPjK^ compared to th e y i e l d from th e NgPj and ThjP-jK^ tr e a tm e n ts may he due t o a d i r e c t o r i n d i r e c t e f f e c t o f an u n b alan ced n u t r i e n t s u p p ly . The d e c re a se d y ie ld from p l o t s r e c e iv in g t h e h ig h r a t e s o f n itr o g e n was n o t caused by d e la y e d m a tu r ity and f r o s t damage. P o ta ssiu m tr e a tm e n ts had a d e p r e s s in g e f f e c t on th e y i e l d . ■She E f f e c t o f F e r t i l i z a t i o n on t h e N itro g e n , Phosphorus and P o ta ssiu m C ontent o f t h e S eed. N itro g e n , p hosphorus and p o ta ssiu m d e te r m in a tio n s were made a c c o rd in g to a m ic ro -c h e m ic a l p ro c e d u re (13) a f t e r a sh in g t h e m a te r ia l i n an e l e c t r i c f u r n a c e . P o ta ssiu m was d e term in e d by th e d i r e c t re a d in g method u s in g th e Perfcin-E lm er flam e p h o to m e te r. o f a com posite sam ple o f seed from each p l o t . The a n a ly s i s was made .'.The r e s u l t s o f th e a n a ly s is a re g iv e n in T ab le 18 and F ig u r e s 1 6 , 17 and 1 3 . a n a ly s is o f th e r e s u l t s i s g iv en i n T a b le s 1 9 , 20 and 21. S ta tis tic a l -3 1 - TablS I S .- The P e rc e n t F itro g e n , FgOs and KgO i n th e Seed* T reatm ent ** F -P 2 0 5- K g O 0 - 1 2 3 .0 1 2 3 0 1 2 3 0 1 2 3 - 0 "0 0 - 0 0 0 0 0 1 mm m - 2 .1 0 2 .3 .. 2.3 2.5 0 0 1 1 1 1 - 0 1 . 0 0 mm 2 .0 1 mr 1 Re =;ular Seed P e rc e n t P e rc e n t P e rc e n t F k 2o P 2°5 0 1 1 ■1 1 - ' 0 • 1 1 1 1 2.3 2 .4 2 .5 1 .5 2 .4 . 2 .5 2 .7 1.8 2.3 2.5 2.6 . .48 .50 .52 .5 2 .51 .50 .5 1 .51 .68 .77 .80 .81 .61 .71 .77 .78 .41 .4 4 .4 4 .4 0 .39 .4 2 .41 .43 .41 .4 2 .43 .43 .43 .40 .45 .43 . S u ck er Seec P e rc e n t P e r c e n t P e rc e n t k 2o F P 2°5 2 .04 2 .3 4 2.36 2.23 1.96 2.03 2.16 2.40 2 .0 2 2 .1 0 2 .1 0 2 .3 5 1 .6 1 1.7 3 2 .1 0 2.1 7 .57 .63 .56 .63 .51 .59 .59 .61 .6 5 . .50 .69 • .7 1 .67 .58 .6 4 .69 .44. .51 .4 7 .59 .41 .39 .48 " .49 .4 4 .45 .39 . .4 2 . .45 .40 .43 .40 * Oven dry b a s is . ** See page 7 f o r tre a tm e n ts p e r a c r e . Table 1 9 .- A n a ly sis o f V ariance o f th e P ercen t N itro g e n in th e Seed (Oven Dry B asis) Source Blocks F P K FP NK PK • NPK E rro r T o ta l ' D .F. . __ . 5 3 1 " 1 3 3 1 3 27 47 - ^ S i g n i f i c a n t a t 5% p o i n t . ■ ^ S i g n i f i c a n t a t lf« p o i n t (+ ) in c r e a s e s ( - ) d ec r e a s e s S .S . 1.0708 15.1338 .0001 .1398 2.4048 .1502 - .0275 .3861 3.4805 22.7936 M.S. .2142 5.0446**C+) .0001 •1398- .8016 * (^ ) .0501 .0275 .1287 .1289 -3 2 - T ab le 2 0 .- A n a ly s is o f 'V ariance o f t h e P e rc e n t P 2 O5 i n th e S eed . (Oven Dry B a s is ) S ource D .F . B locks E P K W m PK NPK; , E rro r T o ta l 5 3 1 1 3 3 1 3 27 47 S .S . .3262 “ .6157 8.9355 .0540 .3747 .0024 .0808 .0299 1.0530 11.4722 M .S. .0652 .2052 ■K-^'4 ') 8.9355- sk=-(+) .0540 .1249 *(+ ) .0008 .0808 .0 1 0 0 .0390 •^ S ig n ific a n t a t 5% p o in t ^ S i g n i f i c a n t a t 1% p o in t C + ) in c r e a s e s ( - ) d e c r e a s e s T able 2 1 .- A n a ly s is o f V a ria n c e " o f th e P e rc e n t KgO i n th e S eed . (Oven Dry B a s is ) S ource B locks N , . , r.-. ■ ......... : P K NP NIC PK NPK E rro r T o ta l D .F. 5 3 1 1 !■> O 3 1 3 27 47 ■ ^ S ig n if ic a n t a t 5 /i p o i n t “• ^ • S ig n if ic a n t a t 1% p o i n t (+ ) in c r e a s e s (-)d e c r e a se s S .S . tr c ivi• O • .4396 .0663 .0091 ~ .0 0 0 1 .0600 .0228 .0140 .0136 .4005 1.0260 .0879 .0221 .0091 .0001 .0200 .0076 .0140 .0045 .0148 - -3 3 ' R e g u la r Seed N itro g e n : Seed produced on p l o t s f e r t i l i z e d w ith n itr o g e n a lo n e c o n ta in e d s i g n i f i c a n t l y more n itr o g e n th a n seed from u n tr e a t e d p l o t s . No in c r e a s e i n n itr o g e n c o n te n t o c c u rre d in t h e seed from n it r o g e n p o ta ss iu m t r e a t e d p l o t s compared to n itr o g e n tr e a tm e n ts a lo n e . P o ta ssiu m tr e a tm e n ts a lo n e had no in f lu e n c e on t h e n itr o g e n i n th e seed. Seed grown on n itro g e n -p h o s p h o ru s t r e a t e d p l o t s c o n ta in e d s i g n i ­ f i c a n t l y more n itr o g e n th a n seed from p l o t s t r e a t e d w ith n itr o g e n a lo n e . T h e re fo re , phosphorus caused an in c r e a s e in t h e n itr o g e n con­ t e n t o f th e seed when ample n itr o g e n m s p r e s e n t . Of i n t e r e s t i s th e f a c t t h a t seed from p l o t s f e r t i l i z e d w ith p h o s­ p h o ru s a lo n e and p hosphorus -p o ta s siu m had a lo w e r n itr o g e n c o n te n t th a n seed produced on any o th e r p l o t . In t h i s c a s e , t h e phosphorus s tim u la te d - v e g e ta tiv e g ro w th , which p ro b a b ly u t i l i z e d m ost o f th e a v a i l ­ a b le n i t r o g e n . As a r e s u l t , t h e r e was i n s u f f i c i e n t n itr o g e n f o r t h e s£ed n e e d s . ' Phosphorus? P o ta ssiu m a lo n e and n itr o g e n -p o ta s s iu ra f e r t i l i z a t i o n ■ had no in f lu e n c e on th e phosph o ru s c o n te n t o f t h e seed compared to seed from p l a n t s r e c e iv in g no f e r t i l i z e r , a l l showed p r a c t i c a l l y th e same phosphorus c o n te n t. The phosphorus f e r t i l i z e d p l o t s produced see d w ith a phosp h o ru s c o n te n t s i g n i f i c a n t l y h ig h e r th a n seed produced on th e o t h e r p l o t s n o t t r e a t e d w ith p h o sp h o ru s. T h is i s p ro b a b ly due to t h e a c u te d e f ic ie n c y o f phosphorus i n t h i s s o i l . R esearch done a t th e V ir g i n i a A g ric u l­ t u r a l E xperim ent S ta tio n h a s shown t h a t co rn grown on s o i l v e ry d e f i ­ c i e n t i n p h o sp h o ru s, produced g r a in w ith a lo w e r p h o sp h o ru s c o n te n t th a n c o rn grown on s o i l w ith " a n ample su p p ly o f phosphorus* P e rh a p s th e -m o s t i n t e r e s t i n g r e s u l t i s t h e s i g n i f i c a n t in c r e a s e i n th e p hosphorus i n t h e g r a in on p l o t s f e r t i l i z e d w ith n itr o g e n a lo n e compared t o g r a in from u n tr e a te d p l o t s ; and th e s i g n i f i c a n t in c r e a s e o f p h osphorus i n t h e g r a in from n itro g e n -p h o s p h o ru s t r e a t ­ m ents compared t o phosp h o ru s tr e a tm e n t alo n e* On th e s e p l o t s , th e n itr o g e n h a s been r e s p o n s ib le f o r an in c r e a s e i n t h e p h o sp h o ru s con­ te n t o f th e seed. H ie n itr o g e n f e r t i l i z e r u sed was sodium n i t r a t e * Sodium i s supposed t o a id i n th e t r a n s l o c a t i o n o f phosp h o ru s , t h e r e ­ f o r e , th e a d d itio n o f sodium may hav e a id e d i n t h e t r a n s l o c a t i o n o f t h e phosp h o ru s t o th e seed* I h e e l e r ( 2 3 ) , e t a l . , re p o r te d i n 1906 t h a t sodium s a l t s a p p lie d t o Rhode I s la n d s o i l s in c re a s e d t h e p e rc e n ta g e o f p h osphorus i n th e p l a n t . Ample n itr o g e n and p h o sp h o ru s i s e s s e n t i a l t o s e c u re t h e h ig h e s t p hosphoru s c o n te n t i n t h e s e e d . H ere a g a in t h e n e c e s s i t y o f b a la n c e d n u t r i e n t s i s e v id e n t* P o ta ssiu m : T here was no s i g n i f i c a n t d if f e r e n c e i n th e p o ta ssiu m c o n te n t o f th e seed produced on th e v a r io u s p lo ts * One f a c t o r i n ­ f lu e n c in g t h i s i s t h e h ig h p o ta ssiu m c o n te n t i n th e s o i l b e fo re t r e a t ­ m e n t. N itro g e n o r phosp h o ru s a lo n e o r i n c o m b in atio n had no in f lu e n c e on th e p o ta ssiu m i n th e seed* -3 5 S ucker Seed N itro g e n : The n itr o g e n c o n te n t o f th e seed from t h e s u c k e rs showed i n g e n e r a l t h e same in f lu e n c e from f e r t i l i z a t i o n as th e r e g u l a r seed* The p r i n c i p a l e x c e p tio n was th e h i g h e r n itr o g e n c o n te n t o f th e seed from phosphorus t r e a t e d p l o t s a lo n e compared t o seed from th e o th e r p lo ts * P h o sp h o ru s: . •" Phosphorus tr e a tm e n ts d id n o t have a s marked i n ­ flu e n c e on t h e s u c k e r seed a s t h e r e g u l a r s e e d . S u ck er seed from t h e n i t r o g e n , p o ta ss iu m , p h o sp h o ru s, n itr o g e n -p o ta s s iu m and p h o sp h o ru sp o ta ss iu m t r e a t e d p l o t s w ere a s higfr o r h ig h e r i n p h o sp h o ru s th a n r e g u l a r seed from s i mil a r p l o t s . The p h o sp h o ru s i n t h e s u c k e r seed was lo w e r from p l a n t s grown on t h e n itro g e n -p h o s p h o ru s and n itr o g e n -p h o s p h o ru s p o ta ss iu m p l o t s th a n seed from t h e r e g u l a r h e a d s produced on th e s e p lo ts * P o ta ssiu m s The p o ta ss iu m c o n te n t o f t h e seed showed no e f f e c t — from any tre a tm e n t* The n itr o g e n , p hosphorus and p o ta ss iu m c o n te n t o f th e s u c k e r seed show i n g e n e r a l th e same e f f e c t s from f e r t i l i z a t i o n a s t h e r e g u la r heads h u t n o t a s c o n s is te n t. The la c k o f c o n s is te n c y i s p ro b a b ly due t o t h e v a r i a t i o n i n t h e m a tu r ity o f t h e su ck ers* -3 6 ; The E f f e c t o f FertL l i z a t i o n on th e F o lia g e C o lo r The c o lo r o f t h e f o l i a g e was d e te rm in e d by th e u se o f th e c o lo r s ta n d a r d s a s g iv e n i n R o b ert Ridgeway 1 s book "C o lo r S ta n d a rd s and C o lo r N om enclature" p u b lis h e d i n 1912 by th e a u t h o r . The c o lo r o f t h e t h r e e c e n te r le a v e s was m atched w ith " th e n e a r e s t c o lo r p l a t e i n th e book. The c o l o r d e te r m in a tio n s w ere made on A ugust 4 , 5 , and and t h e t h r e e re a d in g s a v e ra g e d . 6 I f t h e r e was v a r i a t i o n i n th e re a d in g s on th e t h r e e s u c c e s s iv e d a y s , t h e re a d in g s w ere made a g a in on A ugust 7 , 8 , and 9 . A f te r th e c o lo r o f t h e le a v e s was m atched w ith th e n e a r e s t c o lo r g iv e n i n t h e c o lo r s ta n d a rd s ? t h e c o m p o sitio n o f t h e c o l o r was d e t e r ­ m ined from a t a b l e g iv e n i n th e b o o k . The c o m p o sitio n o f th e c o l o r o f t h e le a v e s a s d e term in e d f o r each tr e a tm e n t i s g iv e n i n T able 21 and shown i r r F ig u re s 18 and 1 9 . A cco rd in g t o t h e s ta n d a rd s u s e d , an in c r e a s e i n th e p e r c e n t o f b la c k and a d e c re a s e i n t h e p e r c e n t o f y e llo w g iv e s a d a r k e r g re e n and an in c r e a s e i n y e llo w and d e c re a s e i n b la c k w ould mean a l i m i t e r g r e e n . -3 7 - Table 2 2 .- The C olor o f th e P la n t F o lia g e on August 5 and Septem ber 1 . Treatm ent * Nk P 205 -K20 0 1 2 3 0 1 2 3 0 1 2 3 0 1 2 3 * .— - 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 C o lo r P erc e n t August 5 Green Y ellow Black - 0 - 0 - 0 — 0 - 1 - 1 - 1 - 1 - 0 - 0 - 0 - 0 - 1 - 1 - 1 - 1 6 6 6 6 6 8 8 6 17 5 4 4 17 4 3 3 49 49 49 49 49 47 47 49 53 32 25 25 53 25 26 26 45 45 45 45 45 45 45 45 30 63 71 71 30 71 71 71 C olor P erc e n t S e p t. 1 Black Green Yellow 11 6 6 6 11 8 6 6 24 6 3 3 24 ■ 6 3 3 44 49 49 49 44 47 40 40 76 49 26 26 76 40 26 26 45 45 45 45 45 45 54 54 0 45 71 71 54 71 71 See page 7 f o r tre a tm e n ts p e r a c r e . August 5 Readings* N itro g en a lo n e , potassium alo n e and n itro g e n - potassium . f e r t i l i z a t i o n had no e f f e c t on th e c o lo r o f th e f o lia g e com­ p ared t o no f e r t i l i s a t i o n * The supply o f phosphorus was v ery low i n th e s o i l and t h e r e f o r e th e n itro g e n was unable t o fu n c tio n p ro p e rly i n deve­ lo p in g c h lo r o p h y ll. Phosphorus i s n o t a component o f c h lo ro p h y ll, b u t i s a p p a re n tly e s s e n t i a l f o r i t s developm ent. — P la n ts grown on phosphorus t r e a t e d p lo ts were l i g h t e r i n c o lo r w ith more y ello w and l e s s b lack th a n p la n ts grown w ith no tre a tm e n t, which shows a d e fic ie n c y o f n itro g e n developed th ro u g h th e in c re a se d p la n t grow th. P la n ts grown on p h o sp horus-potassium p l o t s showed th e same c o lo r a s th o s e grown on phosphorus t r e a t e d s o i l . -3 8 - ETitrogen-phosphorus and sitro g e n -p h o sp h o ru s-p o ta ssiu m produced an in c r e a s e i n green c o lo r o f p la n ts above th e check, n itr o g e n , p o tassiu m , n itro g e n -p o ta s siu m and o f co u rse much in c re a s e o v er phosphorus and phosp h o ru s-p o tassiu m tre a tm e n ts* These r e s u l t s show th e n e c e s s ity o f a b alan ced n u t r i e n t supply to develop th e g re e n e s t color* P la n ts grown on N jF i and K^PjK-^ t r e a t e d p l o t s were a l i t t l e l i m i t e r g reen th a n p la n ts on th e RgPi and UgP^Ki f e r t i l i z e d p lo ts * The p la n t s grown on th e NgP^ IT gP ^i f e r t i l i z e d p l o t s were a s d a * green as th o se p la n ts on th e I^P^ and ETgPjK^ f e r t i l i z e d areas* These r e s u l t s show t h a t 120 lb s* o f n i t r o ­ gen p e r a c re was s u f f i c i e n t to produce t h e d a rk e s t g reen color* P otassium alone o r i n com bination w ith n itro g e n and phosphorus had no e f f e c t on th e c o lo r o f th e p la n ts * The potassium supply i n th e s o i l was th o u g it to be ample in t h e b eg in n in g , which may be th e reaso n f o r th e p o tassiu m a p p lic a tio n s Showing no s ig n i f i c a n t e f f e c t on t h e c o lo r o f th e fo lia g e * Septem ber 1 Readings! There was l i t t l e change i n c o lo r o f th e p la n ts grown on th e check, n itr o g e n , p o tassiu m and n itro g e n -p o ta ssiu m t r e a t e d p l o t s from August 5 t o September 1* There was a s l i g i t d ecre a se i n th e green c o lo r o f th e p la n t s on th e check and th e potassium p l o t s , b u t t h i s i s p ro b ab ly n o t o f s ig n ific a n c e * P la n ts on th e phosphorus and phosphorus-potassium t r e a te d p lo t s Showed a marked d ec re a se in g reen c o lo r as th e season developed* T his i s due t o a f u r t h e r d ec re ase i n th e n itro g e n supply* P la n ts grown on th e K jPi andHiPjKi f e r t i l i z e d s o i l s decreased in g re e n c o lo r from August 5 t o September 1 , which Shows t h a t 60 lb s* o f -3 9 n itr o g e n was n o t s u f f i c i e n t t o m a in ta in th e g re e n c o lo r th ro u g h o u t th e season. P la n ts on t h e Ng^l NgP-^Ki t r e a t e d a r e a s m a in ta in e d t h e d ark g re e n th ro u g h o u t th e grow ing p e r io d , and w ere a s d ark g re e n on Septem ber 1 a s th e p l a n t s grown on t h e K jP j and % P x ^ l t r e a t e d p l o t s . B ie E f f e c t o f T reatm en t on t h e C o lo r o f th e Seed The c o lo r o f t h e seed was d eterm in ed by t h e same method a s th e c o lo r o f th e f o l i a g e ex cep t t h a t a d i f f e r e n t c o lo r p l a t e was used from Ridgeway*s "C o lo r S ta n d a rd s and N o m en clatu re". C o lo r d e te r m in a tio n s w ere made a f t e r th e seed had become a i r d r ie d and i n a dark en ed room w ith a c o n s t a n t - l i g h t • a f te r h a rv e s t. T able 2 3 .- The p e rc e n ta g e c o lo r o f th e seed i s p re s e n te d i n T a b le 23. C o lo r o f th e S eed. Treatm ent * N- P205 -K20 o 1 2 3 0 1 2 3 0 1 2 3 - 0 0 0 0 - 0 0 0 0 - 0 - 1 - 0 - 1 6 1 1 0 0 0 0 *■ * 1 ** 1 1 ■ 1 - 0 - r - i - x 1 X 1 2 3 - JL 0 * 1 X The seed was n o t exposed to t h e sun a t any tim e C olor P ercen t Red - (Orange . 11 25 25 8 7 4 ' 32 27 36 36 39 27 30 42 33 44 17 17 44 48 51 55 See page 7 f o r tr e a tm e n ts p e r a c r e . Yellow ! Black 10 15 15 19 16 15 16 13 18 29 29 «0 45 45 29 25 45 39 45 29 29 34 45 45 45 W hite 29 29 29 29 29 10 29 29 14 -4 0 - As shown by th e d a ta i n T able 23, s in g le elem ents alo n e had v e ry l i t t l e e f f e c t on th e c o lo r o f th e seed compared t o no tre a tm e n t* Seed produced on n itro g en -p h o sp h o ru s t r e a t e d p l o t s were d a rk e r i n c o lo r and ' b r ig h t e r th a n th o s e from th e u n f e r t i l i z e d areas* Seed produced on p l o t s re c e iv in g n itro g e n w ith p h o sp horus-potassium were b r i g i t e r b u t t h i s could n o t be ex p ressed by c o lo r d iffe re n c e s * -4 1 - SUMA4RY AND CONCLUSIONS A uniform a re a o f s o i l (G ro seclose s i l t loam) v ery low in a v a il­ ab le phosphorus, v ery low in n itro g e n and organic m a tte r b u t showing medium to h ig h in a v a ila b le and exchangeable potassium was s e le c te d , to study th e e f f e c t o f v a ry in g th e r a te s of n itro g e n , phosphorus and p o ta s ­ sium alone and in a l l com binations on c e r ta in p la n t c h a r s .c te r is tic s of a dwarf g ra in sorghum. This was th e p o o re st s o i l in a v a ila b le phos­ phorus and n itro g e n t h a t could be lo c a te d . The a v a ila b le and exchange­ ab le potassium was h ig h which i s o fte n found in old u n f e r t i l i z e d p a s tu re s in t h i s a r e a . A 4 x 2 x-2 f a c t o r i a l d e sig n rras used w ith fo u r tre a tm e n ts o f n i t r o ­ gen (0 , 60, 120 and 180 pounds N p e r a c r e ) , two tre a tm e n ts of phosphorus (0 and 240 pounds PgOg p e r a c re ) and two tre a tm e n ts o f potassium (0 and 120 pounds K£0 p e r a c r e ) . The e f f e c t o f t h e tre a tm e n ts on th e p la n t c h a r a c t e r is t i c s i s given in condensed form in t a b l e 24. ■ The E f f e c t o f N itrogen This s o i l was v ery low in n itro g e n and organic m a tte r as shown by th e s o i l t e s t s . No legumes had grown on t h i s s o i l f o r many y e a r s . The l i g h t c o lo r o f th e sp a rse v e g e ta tio n in d ic a te d a lack of n i t 1*0 gen. N itro g en alone in flu e n c e d s ig n if ic a n t ly only f iv e of t h e p la n t char­ a c t e r i s t i c s s tu d ie d ; in c re a s in g th e number o f s u c k e rs, d elay in g th e h e ad in g , d e c re a sin g th e y ie ld and in c re a s in g th e p e rc e n t n itro g e n and phosphorus in th e see d . The n itro g e n combined w ith phosphorus had a more pronounced in flu e n c e on th e c h a r a c t e r is t i c s th en n itro g e n alone as evidenced by th e s ig n i f i c a n t in c re a s e i n th e h e ig h t o f p l a n t s , e a r l i ­ n e s s o f h ead in g o f th e r e g u la r heads and s u c k e rs , in c r e a s e in th e w eight p e r h ead , th e w eight o f seed p e r h ead , th e w eight o f one hundred seed , y ie ld p e r a c re and th e p e rc e n t n itro g e n and phosphorus in th e seed* The r e s u l t s , i n g e n e r a l, show t h a t th e a d d itio n o f n itro g e n alo n e t o a phosphorus d e f i c i e n t s o i l h a d a m inor in flu e n c e on t h e c h a r a c te r ­ i s t i c s s tu d ie d b u t a s ig n i f i c a n t d e p re ssin g e f f e c t on th e yield* Whereas n itro g e n combined w ith phosphorus had a p o s itiv e s tim u la tin g e f f e c t on many o f th e . c h a r a c t e r is t i c s * The r e s u l t s o f t h i s stu d y show c o n c lu s iv e ly th e n e c e s s ity o f n u t r i e n t balance* The E f f e c t o f Phosphorus The f a c t t h a t th e a p p lic a tio n o f 240 pounds p e r a cre o f P 2 O5 had a h ig h ly s ig n i f i c a n t in flu e n c e on t h i r t e e n o f th e s ix te e n p la n t c h a r a c te r ­ i s t i c s s t a t i s t i c a l l y an aly zed ; and had a pronounced in flu e n c e on th o se t h a t were n o t s t a t i s t i c a l l y analyzed shows th e im portance o f t h i s e le ­ ment t o dwarf g ra in sorghum on t h i s s o il* The g e n e ra l responce to phosphorus s u b s ta n tia te s th e c o n clu sio n t h a t th e supply i n th e s o i l was in ad eq u ate b ased on th e r e s u l t s o f th e s o i l t e s t * The d a ta from t h i s experim ent and u n p u b lish ed d a ta from th e Agronomy Department o f th e V ir g in ia A g r ic u ltu r a l Experim ent S ta tio n show c o n c lu s iv e ly t h a t phosphorus i s a key elem ent and p ro b ab ly th e key e le ­ ment t o s tim u la te e a r ly growth o f dwarf g ra in sorghum* 43- Bxe h ig h ly s ig n i f i c a n t in c re a s e in p e rc e n t g erm in atio n o f th e seed produced on phosphorus t r e a te d p lo ts i s im p o rtan t and w a rra n ts f u r t h e r in v e s tig a tio n * The seed n o t o n ly gave a h ig h e r p e rc e n t germin­ a tio n h u t germ inated more rap id ly * Bie s ig n i f i c a n t in c re a s e o f n itro g e n i n th e g ra in on p l o t s t r e a te d w ith n itro g en -p h o sp h o ru s shows a s ig n i f ic a n t i n t e r a c t io n between n i t r o ­ gen and phosphorus and th e n e c e s s ity o f n u t r i e n t b alance* Phosphorus alo n e d ep ressed th e n itro g e n c o n te n t in th e seed* Phosphorus had more in flu e n c e on p la n t c h a r a c t e r is t i c s th a n e i t h e r n £ tro g en o r potassium * Phosphorus i s a key elem ent from ^ g e m in a tio n to germ ination"* E f f e c t o f Potassium P otassium alone o r in com bination w ith n itro g en -p h o sp h o ru s had l i t t l e o r no in flu e n c e on most o f th e p la n t c h a r a c t e r is t i c s s tu d ie d . P o ta s­ sium alo n e and i n com bination w ith n itro g en -p h o sp h o ru s had a d e p re ssin g e f f e c t on th e y ie ld * Seed produced on p o ta ssiu m -n itro g e n t r e a te d p l o t s had a s ig n i f i c a n t l y h ig h e r p e rc e n t m o istu re a t h a r v e s t b u t showed a s ig ­ n i f i c a n t d e cre ase i n th e w eight o f th e seed p e r head* Ih e lac k o f e f f e c t o f added potassium on more c h a r a c t e r i s t i c s i s p ro b ab ly due to an ad eq u ate supply in th e s o il* Ih e 120 pounds o f p o ta s ­ sium p e r a c re added may have caused an unbalanced n u t r ie n t c o n d itio n i n th e S o il which r e s u lte d i n a d e p re ssio n o f th e y ie ld and th e w eight o f •Hie seed p e r h e ad , Ih e n e c e s s ity o f knowing th e le v e l o f n u tr ie n ts in th e s o i l i s b rought o u t many tim es* -4 4 - G e n era l B ie w r i t e r f e e l s t h a t t h e r e s u l t s o f t h i s s tu d y show a ls o th e n e c e s s i t y o f d e te n n in in g a s a c c u r a te ly a s p o s s ib l e t h e p l a n t n u t r i e n t su p p ly i n t h e s o i l a t th e b e g in n in g o f any f e r t i l i t y e x p e rim e n t. By h a v in g c a r e f u l l y checked t h e n u t r i e n t b a la n c e i n t h i s s o i l , t h e la c k o f re s p o n se t o p o ta ssiu m and s tr o n g re sp o n se t o p h o sp h o ru s tr e a tm e n ts can be a t l e a s t p a r t i a l l y e x p la in e d * -4 5 T ab le 24*- The E f f e c t o f T rea tm e n ts on P la n t C h a r a c t e r i s t i c s , P la n t C h a r a c t e r i s t i c s HI 8 0 0 Humber o f s u c k e rs p e r 15r row * 4 P e rc e n t o f p l a n t s i n f u l l head A ugust 5 . P e rc e n t o f s u c k e rs p o l l i n a t i n g A ugust 2 0 , P e rc e n t m o is tu re i n t h e seed a t h a r v e s t* W e i^ it p e r head * ** t PK * MPK 0 $ 0 0 0 0 4 0 0 0 4 4 0 0 4 0 0 0 0 y** 4 ** mm 4 ** 4- 0 0 4 * 4 0 H e ig h t o f p l a n t s A ugust 5 ■** ■+ T re atm en ts K .* HP T HK ’ 0 H e ig h t o f p l a n t s J u l y F 0 0 * 4 * 4 1 I 0 * W eight o f seed p e r head - P e rc e n t o f h ead w e ig h t i s seed 0 0 0 0 0 0 0 W eight o f seed p e r head from su c k e rs. W eight o f one hun d red seed 0 4 4 4- 0 4 0 ** 4 0 * + 0 0 0 4 0 4 0 0 0 0 ¥ 0 I W eight o f one hundred seed from s u c k e rs P e rc e n t g e m in a tio n .<■* + 0 0 0 0 0 P e rc e n t brown le a v e s S e p t. 1 0 . mm 4" 0 0 0 0 0 Humber o f d is e a s e d p l a n t s - mm 4 0 4 ** 0 Y ie ld p e r a c r e ** ** 4 ♦* P e rc e n t n itr o g e n i n seed ** 4 * 4 - 0 ** 4 0 P e rc e n t £ 3 © i n seed 0 0 0 F o lia g e c o l o r A ugust 5 - g re e n 0 - F o lia g e c o lo r S e p t. 1 - g reen 0 - P e rc e n t P 2 O5 i n seed ♦ S ig n if ic a n t a t 5% p o i n t ♦ ♦ S ig n if ic a n t a t 1$ p o in t ** 4 * 4 * 4 **m m 0 0 0 0 - 0 0 0 0 0 0 4 0 0 0 0 4 0 0 0 -D e crea se 0 No in f lu e n c e ■+. I n c r e a s e P ig . 1 . - 50 E f f e c t o f tre a tm e n t on th e p l a n t h e ig h t o f a dw arf g r a in sorghum, J u l y 8 and A ugust 5. L b ./a c r e ^ = - ... 40 In ch es 60 N 120 N 180 N 240 Pg05 120 5r0 August 5 50 i o» i 20 J u ly 8 10 »0 % »2 ; H3 N0 *0 1 Ni K2 V N1 K2 po po ; po : P0 p0 pl P0 P0 P0 pl pl P1 pi Ko Ko K1 K0 % % K0 K0 K0 Ki Ko K0 . K1 T r e a tm e n ts «.■ N2 H pi ' P1 pi Ki K1 “o . Ki F ig . 2 - E f f e c t o f t r e a t m e n t on t h e num ber o f s u c k e r s p e r f i f t e e n o f a d w a r f g r a i n sorgh u m . f e e t o f row 40- L b ./ a c r e . 30 Ni Ng Ns Pl Kx Number 20 = s = = 6Q.U ' 120 N 180 N 2 4 0 PgO g 1 2 0 K gO . I 10 0 N0 Po K0 Ni Po K0 N2 Po K0 NS Po K0 N0 Po Kl i N0 Pl K0 Nl Po % N0 Pl n : Ni k0 Kl Pl Kl n2 n3 Ni Ng n3 Po Kl Po Kl Pl K0 Pl k0 T r e a tm e n ts ®2 Pl Kl NS Pl Kl F ig . 3. E f f e c t o f t r e a t m e n t on t h e p e r c e n t o f p l a n t s i n f u l l h e a d A u g u st 5 o f a d w a r f g r a i n so rg h u m . 90i— L b ./a c r e 80 Nx = n2 = '70 Kl = H N N P 2 O5 K20 I J ®3 = Pl - 60 \ 60 120 180 240 120 \! \ / V 50 40 r Fe rc e n t 30 U 20 10 0 N j> ( 1 I 1 _ _ i_ _ _ N i N2 n3 N0 P <3 po P0 po K (D K0 Ko Ko — i1 1 ■&0 K i n2 n 5 >1 N2 n 5 N0 P0 pl P0 po po ■ pi P1 p i P1 K1 V K1 K1 V ■; K o % Ko K1 . ■— T r e a t m e n ts ! __ _ j , . ___ L„_ F ig . 4 . - % *0 KO *1Po , Kq E f f e c t o f t r e a t m e n t on t h e p e r c e n t o f t h e s u c k e r s p o l l i n a t i n g o n A u g u st 2 0 o f a d w a r f g r a i n sorgh u m . N2 Po KO N5 | po K0 NO Po % N0 Pl K0 Hi Po Kl 2 Po Ki n NS Po % Treatm ents Nl Pl K0 2 Pl K0 n 5 Pl k0 n NO P1 K1 , Nl Pl K1 n2 Pl Kl Ng Pl % F ig . S .— E f f e c t o f tr e a tm e n t on th e m o is tu re p e r c e n t i n th e se e d a t h a r v e s t o f a d w a r f Ig ra jn so rg h u m L b ./a c re P e rc e n t 50- 1 01 0 1 -10 No Nl N2 N3 No No Nl PO KO )p0 Kq Ko Kq Ki Pl K0 P0 Ki 0 Ng N5 Ki Ki T re a tm e n ts Nl Ng N5 N0 Ni Ng Nj Pl Kq Pl Ko K0 Ki Ki Pl Kl Pl Kl F i g . 6 . - E f f e c t o f t r e a t m e n t on t h e w e ig h t p e r h e a d o f a d w a r f g r a i n sorghum , 9 0 .0 6 7 .5 L b ./a c re 60 120 180 240 120 N N N P2O5 KgO G rm s 45.0 2 2.5 T re a tm e n ts F ig . 7 . — E f f e c t o f tr e a t m e n t o n t h e w e ig h t o f t h e s e e d p e r h e a d o f a d w a rf g r a in so rg h u m . L b ./a c re ^ » i : s 60 N N2 a 120 N n 5 a 180 N - 240 Pg05 - 120 K20 6 7 .5 — Grins; 4 5 . C* i U1 I to 22 . N, i- ~ 1 .......... 1. K1 N2 N5 No V N1 po po K0 po ,K0 po K1 P1 K0 P0 Ki Kq N5 N1 P0 po K1 % T re a tm e n ts P1 K0 N2 n2 P1 K0 *5 Ho Ni P1 Kq pl K1 pl K1 N2 P1 K1 H5 P1 K1 F ig . 8 . - E f f e c t o f t r e a t m e n t on t h e p e r c e n t o f h e a d w e ig h t i s s e e d o f a d w a r f g r a i n sorgh u m . L b ./a c re Nx = 60 N Ng = 120 N N3 - 180 H P l s 240 pgOg % = 120 KgO , 80 P e rc e n t i to I cn 70 60 H0 Po Ko ..*1 Ng H Po Ko Po Ko Po *o No Po K1 % Pl Ko Nl N2 Po Po K1 Po K1 K1 T re a tm e n ts K1 Ng Pl Ko Pl *0 Ni Ng Pl Pl 1 Pl Pl K0 K1 K1 N0 K1 *5 Pl K1 F ig . 9 .- 1 E f f e c t o f t r e a t m e n t on t h e w e ig h t o f t h e 1s e e d p e r h ead from, s u c k e r s o f a d w a r f g r a i n sorgh u m . i L b ./ a c r e 30 Nl «2' 20 Pl Kl — 60 120 ST 180 240 120 N N n Pg05 KgO Grin. 10 NO Nl n2 Ns Nq N0 Nl Ng N« Nl Np Ns Nq Ni P0 Po Po ' Po Po Pl Po Po Po Pl Pl Pi Pl Pl Ko % K0 ' "% K1 Kl K0 K1 Kl *0 K0 so T reatm en ts K0 X0 ' Ng N* t.- Pl ?! Ki Kl F ig . 1 0 .- E f f e c t o f t r e a t m e n t o n t h e w e ig h t o f 1 0 0 s e e d o f a d w a r f g r a i n so rg h u m . 5 .0 2 .5 L b ./a c re » 2 .0 Nx - 60 N N2 - 120 N N5 i 180 N P l - 240 PgOg Kx = 120 KgO "" 1 .0 N0 N1 n2 N5 N0 PO PO Po Po *0 Ko Po Ko N0 Pl Ko K1 V % Po Ki n2 Po K1 n5 Po K1 T r e a t m e n ts Nl Pl xo N2 Pl Ko n5 Pl Ko No Pl K1 Ni Ng Ng Pl Pl Pl K1 K1 K1 F ig . 1 1 .- E f f e c t o r t r e a t m e n t on t h e w e ig h t o f 1 0 0 s e e d fro m t h e s u c k e r h e a d s o f a d w a r f g r a i n sorgh u m . I UT1i C I L b ./a c re 60 N 120 N 180 N 240 P2O5 120 KgO 0 '•0 N0 N0 % n2 NS Ni n2 N3 P0 P0 P1 P0 P0 P0 V P1 P1 K0 K1 K0 K1 K1 K1 T re a tm e n ts K0 K0 K0 N2 K-. P1 Ki »3 P-, Fxg. 12 - E f f e c t o f trea-faaent on th e p e rc e n t germ in atio n o f a dw arf g ra in sorghum J ' ' ■: / 100 P ercen t 90 L b ./a c re 60 N 80 180 N 240 Pg05 120 KgO 70 »0 H, T reatm ents F ig . 1 3 .- E f f e c t o f t r e a t m e n t on t h e p e r c e n t brow n l e a v e s S e p te m b e r 1 0 o f a d w a r f g r a i n sorgh u m . Nx = 60 N N2 = 120 N N5 = 180 N P l = 240 P20 5 Ki = 120 KgO 0 N0 Ni n2 NS N0 N0 Nl n2 n3 Nl Ng N5 N0 Ni n2 P0 P0 po po po P1 po po po. P1 P1 P1 pl P1 P1 K 0 K0 K0 K0 K1 K0 K1 . K1 K1 K0 K0 K0 K1 % K1 T r e a tm e n ts n3 , i P l -u % p i g « 1 4 . — E f f e c t o f trea-hn*»»> + <* » * » r f g r a i n t ^ W * °f P ^ t a p . , 30 f o o t ™ L b./acre N1 r . n2 ss n3 « P1 = % = Number 60 N 120 N 180 N 240 P 20s 120 KgO 1 tn < £> I V Kr Kr »3 po *0 *o po K1 »0 P1 Ko Ki T r e a tm e n ts '3 *0 1 »1 P1 *0 *2 pl K0 % p1 K0 Ki "3 P1 Ki F ig , 1 5 .- E f f e c t o f t r e a t m e n t on t h e y i e l d p e r a c r e o f a d w a r f g r a in , so rg h u m . ] j , |■ L b ./a c re 60 120 180 240 120 20 *0 PO K0 N N N P20 5 KnO 1 1 r 1 1 Nl PO *2 NS H.o PO ' Po Ko Po Ho Pl NO % N0 Ki 1 1 1 I | <1 | | f *1 n2 n5 % Ng % »0 Ni Ng NS Po Po Ki Po Pl Pl Pl Pl Pl KX K0 K0 P1 N0 Pl K1 % Ki % ‘t Nl T rea tm e n ts F ig . 1 6 .- E f f e c t o f t r e a t m e n t on t h e p e r c e n t o f n i t r o g e n i n t h e s e e d o f a d w a r f g r a i n sorgh u m . 5 .0 2 .5 L- 2.0 P e rc e n t % Ng N3 Pl Kx 1 .5 = 60 - 120 = 180 = 240 - 120 •19- L b s ./ a c r e N N N Pg05 KgO 1.0 NO Ni ; po •' K0 po Ko n2 po Ko N3 po Ko N0 po K1 N0 P1 K0 N1 po K1 W2 N3 po po K1 K1 Treatm ents Ni K, Ni Ni K, Nr Ni Ni K, Ki N* F ig . 1 7 ..80 E f f e c t o f tre a tm e n t on th e p e rc e n t o f PjjOjjin th e seed o f a dw arf g ra in sorghum. L b ./ a c re .70 P e rc e n t .60 .50 .40 T reatm ents F ig . 1 8 .- E ffe c t jof treatm en t on th e p ercen t o f KgO in th e seed o f a dwarf g ra in sorghum. .60 .40 I o> to I L b ./ a cre P e rc e n t »i n2 .20 : t : NS Pl Ki1 = - N0 % Ng P0 Kq P0 % P0 K0 ■V po Ko po N0 »i pr l ^ o h K0 N0 Kx Ng, N-3 % Ng P0 P0 Ki K1 T reatm ents P1 K0 P1 K0 N3 P1 Ko N0 P1 K1 60 120 180 240 120 N N N Pg05 K20 % P1 Kv Nr Ki n5 P1 *1 F ig . 1 9 .- E ffe c t o f treatm en t on th e amount o f b la c k , g reen , and y e llo w in th e fo lia g e on August 5 o f a dwarf g ra in sorghum. 80 P e rc e n t^ B la c k P e rc e n L b ./ a c re 60 N 120 N 20 ± 1 2 4 0 P 9O5 120 KgO .Permit. G re e n / \ P e rc e n t Y e llo w T7.:,.zn T reatm ents F ig . 2 0 .- E f f e c t o f tre a tm e n t on th e amount on September 1 o f a dw arf g ra in s< b la c k , g re e n , and y ello w in th e folialge L b ./a c re - Ki Ng Ng Pl Kx = s - 60 120 180 240 120 A N N N P2O5 KgO / / \ / / Black r - V \ \ / \ \ Green I Yellow 1 0 0 0 N1 p0 Kq ; 1 % p0 Kq 1 1 v 1 1 n3 p0 Ko n0 p0 Kl Nq pl K0 Nl p0 Kl Ng p0 Kl n3 Nl p0 % pl K0 2 pl K0 n NS N0 pl pl Kl Kq Nl pl Kl Ng NS pl pl Kl % -6 6 LITERATURE CITED 1. BAYFIELD, E . G . 1 E f f e c t o f f e r t i l i z e r s upon t h e 'g r a i n t e x t u r e and p r o t e i n c o n te n t o f'-w h e a t. Ohio A gr. E xp. S t a . B u i. 516: 2 9 -3 0 . 1933. 2. ' S o f t w in te r -wheat s t u d i e s . I I I . some f a c t o r s in f lu e n c in g v i s c o s i t y and p r o t e i n . 12: 1 -1 6 . 1935. Hie e f f e c t o f C e re a l Chem., 3. . ■ • • . 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