THESIS
THE ACTION OF WHITEWASH
BNE TEN
I, W. RIGTERINK

vane
SS _ + +--

a
Irn oO
bliss be

TUR ACTICH OF WHITNWOF ULPCh TACTERTA,

 

 

 

a7 9%

flehj can Aerievulivrena’ Gul deicg snenew

~ ~ vy
THESIS
Tite AC'TEC: Cr VCITBWAS UPC DACTHRIA,

 

on boii vee to Getver.ine, if pescihle,
wiether whitewasn (Ca(OH )s) rogsesses civ cisinfeetine or gomsicicéal
rram erties, Whitewosn was i reperca bv
oxice (Cad) in en Msmaren cish, Tris vas then thoroushly slacked by

Girect avvlication of boiline vator wold tn oe peor vyreviouslyv sterilize

’
'

“M14, 7... 4 =~ 7 . s+ . > 5 45 vey RN = 5 4. a 4 -- co wy
ree evv ect cousins bolline water wos to Caecilitetve tie sleckine of the
a9 —- = : ewes N Yoe rans 7 + ‘ an 2 os 7. wr : ~: s ~- ~ . =4- . =
lime, Caleta cxiege vrovent in eenteect vith vater Torus Caleivm hnyvdrate
T Ne 7 2 - Ve - pee = ~ _4 e- Ie - - ~-,7 y3 a
(ca (ONY), Caleium tryerate exresee te air will take ur caruon cioxice

(C02) are chanse to caleium carboncte (Cacos’)., Nut in all the following

r ” *’ 3 . - - _ ee Ne aan =
) Vas us Ca Li Qa Ore Qi C ae Le t 2) a car Ww ou

tN

exyerinents writewash (Ca( Oi)
ete nad taken vrlace,

~

A Maker cP cover-mlasses were cut into ralves end sterilizez ina
Q
Rrot-air oven at 150 CC, vor one hour, Also white silk thre .¢ was eut

into secticns of one inen each anc stecrilizred as above, A Loudlion

ry
iJ
©
QO
f+
t-4
}—!
3
C3

culture was wade frou a melatin culture o Antnracis, This vas

ylacec in an ineuvetor for twenty-four hours, A heneinge arov, fro the
culture revealed the vesetative form of Racillus Antnracis, Wo srore

~—

form of the avove bacillus was observed,

4

Thirivy «
film of this culture, leaving the extreme iwargins of the cover s#lasses

free Trom merms, This was aqone in oraer to make sure tnat the ontire

“ the sverilized cover glas.es were now coated with sn cven

-
U8
nN

film could be covered wen wnitevasn wes arplied, These were allovad to
cry, and tuen, as necrly as possivle, an even coat of whitewasn was
applied to the entire surface oF the cover slassues wy means of a elass
rod having a romdaed end, Thirty cover gslassces vere siven a esatine of
tie whitewashn only to serve as coutrols,

Alsc thirey of tre sterilized silk tnrcacs vere tiorousnlv soaxec

@au.oved serar

whnien atel

="
+

Ta Ped Li

~

Threr<

Vere ~re
onlv,
Tre
order to
one-half

YeMmovec

yale

pouillon tuve Ht ey.

2

Q)

teanen tos

~_~
‘et

co
hd

cover glass: controls were  vrov are

-

c ~~ te
woah?

tavle result co

4.

wnitevesh, T:

n° 3

ho

fol: Me

)

cifforent resvlt in varvi

,)

Thitewasn

hy

V bv means of steril

oer” 4

Phas

yrrr ae

ave C-

it¢

Pili: on the cover elass, and in

the jn cornfinenent,

«a

boulilion tube,

> - ..
Lt at
a or CaN ‘

s
-

a
wd

over tre south cco

e
«

4

oo ” " Fr
rec.

ith eneothner sterilize

mre A
Cet. te
4

en

o:.t

L 4 *

’
da

+ a
te wd

T

ee

rec W ture, (

woe
aaty

don Varen lith., The foliovi

ar t3
Ai. &

Torii of rax witi

7

Ci

ized foreeys,

i
-
( a

~ . “ ‘ eo 4.
ry 4A ‘) oh o> ‘ om e\ of. So wae
Or deo. OT, EY 4 AY ay OT ue, IL OVaAL,

“Ath ceverelac|eas 2 és OX rep. “3 10
VTC SwVr ce,

Result, - - - - - =
Wugnver oOo, comtrois, Pe “ 1 1 1 5

Result, OO gq Yr ow t NA -----------

With lie serayp: ed ort 3 AX ox ‘s 2 HXbD 19
COVOETEIASOVUE

Result, - - - _ _ _
Dlunber of controls, “2 w2OX 1 1 1 E

Resul ~Q srowtn,Conutaing- fi O . Yr O W t 4

cr
e

erovtia or some Kind, With the excortion of th

p~te
a}
Q,
}-
©
65
ch
CL
0)

x

~

> controls,

3

cea n of tie avove tubes was plate

,

m™ s 4. e a4 —™ +r L ~ Lose -, - * * 7 d. ’ asa _
Ax Dortauinated in one tube, cue vo mounlas,

Ox t it It ! "f tt wi er

o)

Oo
p-t-
e

Coc

a

\ ad *.
Cy N ut tt i" " 2. srort v

©)

cilius,
ax " " " " "oyterococci,

ex % YW i! iw tt " iiou 1 Ca .

a 8 ane mo 4 ,

" " eortanminaet ons of abvove “
' . Af ga 4. - 404 5 rs a ~v44 4 yor - ~_Ae

Her Woeontrols treated with whitewash culy 10

i"! wta- 4; 4 “ .
" contaninations of avove, 1.
 

 

a.

0:

No,

11

as

«4

bs

2
~

6.

€

~

iL.WGT OF

sult.

a-
L.

W

C5

W

Result,

2
4

“ore

¢
i

4

WO.

.

wert

3
a

ver
J
e

“3”
Lis low

tv

en

t:

C4

oO

All

oO
AN
(—

{-

ce
«2

t
oe

Vr ym
y. (OF Ma

form

de

‘

™~
”

(

me

4
be
cv
- ew Sle

flared

wre en oe
ny

si
be 4S

9

.)

A
,o

¢

‘
_—

a& Slee.

o-

mT
coll,

-~ Ff ce

t.

Ot.

PL Ll i an
 

 

 

COee vee Vie gre. €1. €f €Y Cviivre (6 vite, fee Vet €cccd € Corer cT
Ceoleivir Pycrete vrielh Gg evricecc te fecilivcve wie grevii cr cvcres,
SOPeore ive vbw ne ce Prey vite ev iwyre revecive favre fi td gberes, Tre
ceevergsicce oc 1d their ecrtreis wore vecce cr. Varel~enecj; the threece on
Tore? 24th, Tre Pelicvirne teele iicicetvce tre reevli,
Tine of trea 2avlc:
Ter 5, OY! | OOP TATE... i. ey 4, You.al,
OP Meviltion wu ce, .
Ogee ae CO Lie CVU EG C.
weed se yromy Te or a i ba “ “2 10
wee det ce,
Result, x x x x x
Mo, of ecntresls, 1 1ax 1 1 1cax 5
Result. “Oo erovwtn Coote. vo srovth --Tontaninated,
ho, cf tures inoculated
vite Tine seravvod ef? “3 2 és ‘ ‘3S 10
ecver elaccws,
“esult, X x X x x
Wuuwer or controls, 1 1xb 1 Lex lex 5
Result, No erowtn ,Coutemin- 7.0 grorvth,contcininstecd,
Otad

Plates were wade oF vach of tre avove tuoec, not ineliudines the
controls, Five of tie controls (ax,bx,cex,adx,ex) were contauinated,
Contamination cf tre controls vias cue in each iustanese to soue Siort
bacillus, Tne 27 lates revealed typical anturax colonics, vangive drors
2200 PPO ticse colonies, further revealed typical Racilli A:-ttrecis,
The next table il:ncicates vork with soctions of threacs,
o

Tinie oF di

of “ovlll
iO, Of
threaa wil
Result,

Control,

Result,

Plates

exceytion

ine

Ou

ally

tre

On

them in

are

‘dae

cr-<

infectcc

end tr

aT

seebions

a % ,
bak

iy ."
“

eantnorax

culture

ATYTil 4th

a

eonvaet

“ peud

-

OL

GPS.

” a+ “ye

weulation
rset ga ace. ae
wee wll, atl’ ets ~ «6

 

(5)

~" (of 7? r « “7 .* a
oO uk ‘ 7 rN
- tre ofr) 6 * ¢ - cil wre eft L e - LV “x e
© fav ?
a cox 63 be

2 2 3 ‘3 ed
Tonta.irnted I O fr Y O v? t n,
of these cultures Svcow typical anthrax cslonies, with tho
cf the evltures incce of “Maren B7trn.,. These cultures were corte.
soue aetively icotile bacillus whien Lliocuirticd selatin beProre

=

~TWarea,

S, Troe

Exreriucnt

~
Ca

nuinper of

S

i\

Oo cL a . mY

vr

f.

1

te

eultures rave

.

1

1

renelnie croy

l.-
my

S

™~
_ men 7.4
: 1 S .. ah C. wm

Oo
ct

rcsitive result of erovtnh cf antnrex,

° em 3 oe 4 * as *
' 5 met CS Laniliched,
tn RBecillus Tuvlereculosis,

~

taread invectud DV erinelns

contact with the tuberculous iwaterial, The ucterial wes taken
ines pie havine tuberculosis, An incision was mace ints the

a
a5 ’

elan

Co

cr
wy +

bv iCal. Ot Yr ot at i on 9

with the ma

Cxnwineaticon at
oe es : 7, os
tivealvs vere tren trea

tericl,

Porects,

- an om wi. : . ~ we e . ay, . wa &
erv yart -f the thread vas Lroueht intec
+t “. “ - 7o- + 4 ™ - 4 “, * *% *

Tt fad woe reviously oestvaclisiiea ov
a7 ” o a ae *¢ oy?
e tubcrele vacillus was present.

. 7
IGuVagdg

imGliiey ,
(7)

* ’ co “7 - a yee te yt CAN o ro ts ae ~ ON ~
A nouxeeer of threacs rere not trerted witli vinitevasn te serve as econtrcls,

On Avril .ié, two euirea-ries were inceulatec on tne inside of the trish
With threacs treated ~ith weiteveshn, A thira euinea vie wos inoeulsted

’ ty. ~- anes 74 9 / 44. . a mI. | -- 2 5., sy, , ey Sat:
in ea similar wanver with a control threac, These vies vweru weiteted cvery

- %e
Y COw e

i ve ‘ 7 “y oye aa . reale A ~ .y 7 ry 1. Vy eee
lst WOoOa e wd V Cvuas e eJZA KL Wetec e ‘;: th ¥ Cato aN e St . \ ( a @ Stny e
-- wy a mr yn ‘ 2 ir 2
cquinea-pig sl OO grams 577 ¢7amf Ble 598 on 634 O50 «
" = ~ cy He ‘ re
u uw i, J vr) “ 5 16 oe HO 2 e 5 ia, ’ a) 7 a 615 ee

i noe 38 vs CTS Vee! 717 716 TiO a

On Juxe 36a thev were iillled and exanmlneca, Not a trace of tubercu-

losis vas diseovered, The tnree guinea-jiss wore a.yarsntliv perfectly

healthy, It is a @iffPicu:t matter to account Tor suc: a result. There is

"

no threacs used nad anv tuverele bacilil

rty

c+

-~ 4
,

a rossibility thet none o:
present, It wav also .e  ossitle that the tuverele vacil- us was custroy-

ead by use of viiblewasn, and thet it was not yresent dn the eontrol,

«

Mxyeriments with Pacitllus cf For Cholera,
Cover slasses and threacs with tcelir eoutrels wore iwace Proua

bouillon culture 24 hours old in the usual wanrer,
Tine oF Lnoculation

’ =. 4. , (yo 5 mc —P re 7°, — 7. oy
. ‘ y wn aie mw” -. aN OP 4 _&s ar wat
Ol, ele ° AY I etsy - « ae [= 200 ° - Ach Y 8) 6 tL ‘J - as 0 9

No. er turvesinoculated
with ccver elasses Pa 23 2 0 4
MREDSUIY ae,
.
Racist _ ~ _ - _

Munver of controls, 1 1 1 1 2
Results, 1 0 G r ° Ww t hoo,
NO, OF cover #lasses

with lime reiovedc, 2 2 0 2 4

Result, - - _ - -
Control, 1 1 1 1 2

Result,

«i4

4

9Garow7wt ii, ---- Contan nated No srowi;
Time of inceulatio
Of Foudlloi: tue, ATTY .37. ADT .29, “av Ss, “av Oo, may QC

rwo, cf secaticns cf
threeca with fern, Pe 2 Pe Pa 4

Result, _ _ - _ _
Control, 1 | 1 1 1 1

Result, uv O G Yr O VI t h  ,.-

Tnese cultures were alloved to rewcii: in the ineuvctor for tan devs.

Vo wrowth could be discovered, and in the ylace of vlating, new voullion

cultures Wore mede from each tube. Of the thirtv-six cultures not a

single cne nad growtn., Of the seventewn controls, one was contamin~ ted,

*+-

It was teougnt tnat the “acillus of Hog Cholera iwisnt be wore nisnly

rosisteant if taken fro. en old culture, To deteriine tnis, cover glascus

to

and tureacs wore y;repared from a culture tihirteon davs old, These vere
(9)

used on Mav Sth anc goth in the acove exrerium t, The result did not
qiffor erou tne tuvcs inoculated witn cover lasses and tnreacs prerarud

froa tie culture twentv-four hours ola,

wxTeEriisent with staphylococcus Pyvorones Aureus,

- an -_ x ~ wwe . . J. r= s . . a 7 . - . we
Cover mlasses ana threacs with uneir controls Were wade Pfriia

bpouivlion culture twentv-Cfour Nours old in the usual wanner,

Tine oF inceulaticon

of “oud tion TULO, may 10, May li. May le, Mav gO,

~o,af tures inoculated

with cover seiassces
uraistur. Ue,

Result, _ _ xX _

Nwuucer of control, 1 1 1 O

Result, a O Gg Yr O W t h :
o,of tubes inoculate

with ltite of COVEN _ 2 2 ‘2 Bax

fr Tasces Be wd e

Result, - - - -

Nuuber oF Controls, 1 1 1 2

Result, r O a Yr Oo W t i .
Tine of tnucculation

of Toutllon tuve, may lO, lay ll, Mav, lo. Mav 2o,
Tio, of sections of

threacs With eoriis. ‘3 2 3 7

Nesult _ - - -

Control, 1 1 od “

Tnese tices vere ailoved to roueain in the ineubstor Vor ton) davs,
Bouil:

O71 tULcs Were then wede Frou .acn culture, One tube vas contanmin-
“ted, Tre two eultures mace oo) May lott. fa gproviun, Pplaves were wiadea
Tren Viese aod Gevelo ed evarneturistic esienioc oF goidon ows, Haneins
Ccreyv eee resvlieew Of Microeceei, Myvavir clit aneiecaved thct trew vere
Prey Toss Jeo vus, Tris iar yrs cote og ov. ta oo Paet that thor
foo oo veg P.bblLiy tral Scie fueriie Pah OT uk eth ly G7 Bet cova
ones rer Oh wate TO

mee bbe wie beet igs onde ia Alis,

Cover g2ece us cio tlretce Viir ipeir ccoiriis vere .reparec Pre c.

Wevjller cviivre tre: ty-fPeur Fevre clic it tie svel wericr,

a e + °
ore -cepieticn:
Trae _ Le cVva'd on
af mae . 44-7 e-ANe: 14 Tone 1
‘= L Ne 1 =. “~ i ~ se ul: te (:! @ - oN fe e 2 re . e
vs! 0 7 - 4 yooed 5 ~ qe co. 4. -
7 tc ae it hho aarti Gk 16 el C.
« 2 d4i.° >, ee Se ee, one ‘oe &
| fee (_ ~ log i Cea ae a ,Q iu 2
a
-- — ad. -%
» . on. a
oan! we Re ~ rw he e
Reerlt _ _
I} eli.

TO. of

No,

with lire

cever mlasc:

Result,
To,of

Result,

Result,

4 4

Contrel

Result,

controls,

of tuikes

coMmwurwls,

-O.,0f sections of thread w

O t

v e

inoculated

a ,” ee. < ,
Yeiacved Trou 2 7 DX
oN +o

hy

}

7m

.
To
vaal CARAS
Cont

Plates frei these tyro colonies
Yvpni Abcciuinalis
WeolGet OF alCGings whitewasn

inoculatec

wT

Yure, ; ee. oo ~ aes
ae ox Limeonts 'or us L ern STs ’

toa

(ould prevent erowth cf Bacillus

Aureus, and Sacillus Ty-nl

inoculated with the avove serics

three, four, and Pivoe

Ir case of Babillus cof Hoe
Aureus, tyyical sro vas found

two, and three loop-fuls of

ound in tne tubes to

rh

se N Oot
+CAw

ey
rs
°
©

LeW bouillon tubes were rade

These
rour days, Tre
W n 1 t OW

~
a

Wea

tubes in the ineuvatcr ton aays,
Couclvsictn: Whitevasn rrever

of Tacillus Antirecis,

Aureus, exce t in tyvo cultures

iad not revesrl colanie

to

andominalis,

WV (Ox. 2

whitevash,. In

ey - %
Woien

tuves

Qv0VO exrerlinent

BQlied to preserve tne necesseryv

Pocillus of Hose; Cnolera,

ry
as

AQ jliecrocoeccus,

o™"
Ls

thread Pormingaci

ypouillon tuves arter

1onwith sorus,

S 2 Of vorot

121

en

Ca

“a
a,

pouillon tube after it

of Hom Cholera,

To each of five

7

Yo LN

loopfuls of writevasn,

in tie tuces to which

rT OF
&. Cob eO

anaea one
iffevrent cultures

were taxen out of

4
[wr

not

InayV

on
wha

rrevertea srowth in

crecaution

growth cf the

=

noted, racillus

of

aviv

sta hnylococcus

bouillon

resrectively,

Vi

veratative

on YR yan *
AvG7iinelis

(11)

llus,

Bacillus

. 8
W111

Q anthrax, to

-

was Irnoculate:

Pyovrenas
4eqel- -
Lubes

one, two

Crolera and Starhylococeus Prosencs

AS anced

one,

case of Typhi Abdoulinalis,

and tyvo loovfuls,

whien gave the

the ineveator
oSielusivelyv

some tubes,

OF leavinges the

Porn
POLES

There
(12

Shitewash was brousht in direct contact with the sermus, Wnitewasn dic

en a . -. de a % “A, e . - -- . Tom aA gy : ay 4 47
not ~wreyvent the erowtn of srore Tovis of “~acilius onthrecis,
(y ald Goose
ICHIGAN STATE UNIV LIBRARIES

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