Population dynamics of Erwinia amylovora have been utilized for many applications over the past 50 years. In this work, populations were tracked over the course of apple bloom under naturally occurring environmental conditions. Flower stigmas inoculated on the 1st day of being open can harbor large (107) populations after 3 or 5 days post inoculation, with 100-fold increases in E. amylovora stigma populations observed in atmospheric conditions with daily average temperatures near 14oC. These large 100-fold increases seem to occur at night, indicating that Erwinia amylovora is able to infect flowers in colder field temperatures than previously reported. In tandem, timing of antibiotic application relative to E. amylovora presence on flower stigmas had little impact on population dynamics, with streptomycin and kasugamycin consistently reducing populations while oxytetracycline was more variable. This study also led to the identification that Kasumin is prone to photodegradation. Culturable bacteria, yeast, and fungal populations were assessed over the course of bloom in relation to application of a biological control agent yeast (Aureobasidium pullulans) and a contact sterilant. Though populations fell rapidly directly after application, by 24 hours all populations returned to pre-spray levels. In regards to further systemic spread, a type three secretion system effector was indicted in blocking the abscission of infected flowers. The use of prohexadione calcium (Pro-ca) and acibenzolar-S-methyl (ASM) reduced internal E. amylovora spread in a seemingly synergistic manner on young trees, with rates of 28.3 g Pro-Ca + 28.3 g ASM or 56.6 g Pro-Ca + 28.3 g ASM. The sum of this work gives us a better understanding of E. amylovora population dynamics in field conditions as well as the population response to current treatment options.
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