Regulation of chlamydomonas reinhardtii cell cycle during synchronous growth
The regulation of the cell cycle in microalgae has long been a central topic in third-generation biofuel studies, as it relates to biomass accumulation and lipid production, the two determining factors of economically feasible biofuel production. The discovery of Compromised Hydrolysis of TAG 7 (CHT7) in the green alga Chlamydomonas reinhardtii has provided valuable insights into the metabolic status-dependent regulation of the cell cycle. CHT7 belongs to the CHC protein family, members of which include transcriptional regulators programming the initiation of cell division. A similar role of CHT7 in mediating cell cycle progression has been suggested. However, the regulatory mechanism and specific processes regulated by CHT7 have yet to be determined. In this dissertation, I applied bioinformatics approaches to comprehensively study CHT7-mediated gene regulation during the cell cycle using cell cultures synchronously grown in bioreactors. I found that specific pathways such as DNA replication, chromosome condensation, and spindle assembly are affected by the absence of CHT7. Other affected pathways include cell-wall remodeling and previously uncharacterized putative kinase cascades. In addition, I discovered the presence of two potential cis-regulatory elements near the transcription start site of misregulated genes in cht7, which are potentially linked to homeodomain transcription factors. Moreover, I explored the potential relationship between the CHT7-mediated pathway and the gene network governed by the retinoblastoma protein complex. Lastly, I characterized the function of a novel CHC protein in cell cycle progression and compared it with the role of CHT7. Together, these studies facilitate a better understanding of the cell cycle regulation in microalgae.
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- In Collections
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Electronic Theses & Dissertations
- Copyright Status
- In Copyright
- Material Type
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Theses
- Authors
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Lin, Yang-Tsung
- Thesis Advisors
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Benning, Christoph
- Committee Members
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Arnosti, David
Farre, Eva
Takahashi, Hideki
Kuo, Min-Hao
- Date
- 2022
- Subjects
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Biochemistry
Molecular biology
Cell cycle--Regulation
Chlamydomonas reinhardtii
Bioinformatics
Biomass energy
- Program of Study
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Biochemistry and Molecular Biology - Doctor of Philosophy
- Degree Level
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Doctoral
- Language
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English
- Pages
- xv, 175 pages
- ISBN
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9798438748106
- Permalink
- https://doi.org/doi:10.25335/xfm4-nm07