ABSTRACT EFFECT OF TWO METHODS OF DRY HEAT COOKERY ON PALATABILITY AND COOKING LOSSES OF SEMITENDINOSUS MUSCLE OF BEEF ROUND by Anne Louise Douglas Shaw This investigation compared the effects of conventional oven— roasting and a combination of internal radial heating and conventional roasting on the palatability, yield, and rate of heat penetration in semitendinosus muscles,comparable in shape and weight, from six pairs of U. S. Choice steer beef rounds. A thermal rod used in the radial-conventional method consisted of an electric wire mounted in a hollow, stainless steel rod 12 inches in length and O. lZS-inch in diameter. The extended wire was termi- nated at each end with a small clamp used to connect the rod into an auxiliary electric circuit. Roasts were split lengthwise to the sample radius for insertion of the rod. For both roasting methods, a controlled air temperature of 149°C was maintained throughout cooking. In addition, a powerstat, set at 20 per cent, controlled the voltage entering the thermal rod in the radial-conventional method, Time-temperature data during roast- ing and cooling were obtained from thermocouples positioned at sample depths of 0. 25-inch, 0. 75-inch and 1.0 inch for the radial conventional method and at depths of O. 25-inch, 0.75-inch and radius for the con- ventional method. Cooking periods were terminated in the radial- conventional method when all positioned potentiometer leads registered Anne Louise Douglas Shaw a minimum internal temperature of 800C and in the conventional method when the lead positioned at the radius registered a minimum internal temperature of 800C. The results of this study indicate that method of cooking does affect palatability, cooking losses, and rate of heat penetration in semitendinosus beef muscle. Total cooking losses and volatile losses were lower and drip and volume losses were higher in radial-conventional roasts than in conventional roasts. With the exceptions of tenderness and residual connective tissue, palatability scores for all factors judged were comparable for the two cooking methods. Conventionally cooked samples were signifi- cantly more tender and had less residual tissue. Total cooking time for the radial-conventional method was approximately half that required for the conventional method and in both methods the rate of rise in internal temperature decreased as the internal temperature of the roast increased. Radial-conventional roasts exhibited a faster rate of heat trans- fer at all times during the cooking period. Both methods exhibited the phenomenon that rate of heat penetration decreases as muscle coagulation proceeds. This study indicates that the radial-conventional cooking method has potential as an acceptable means of cooking semitendinosus muscle of beef round. Modifications of procedure are suggested which might increase tenderness, lessen residual connective tissue, and further improve juicine s s . EFFECT OF TWO METHODS OF DRY HEAT COOKERY ON PALATABILITY AND COOKING LOSSES OF SEMITENDINOSUS MUSCLE OF BEEF ROUND By Anne Louise Douglas Shaw A THESIS Submitted to the Dean of the College of Home Economics Michigan State University in partial fulfillment of the requirements for the degree of MAST ER OF SCIENCE Department of Institution Administration 1964 .. .2 i: ‘r’ 7 ,;/"<—'( AC KNOW LED GM ENTS Sincere and grateful appreciation is extended to Dr. Grace Miller for her patient encouragement, her invaluable assistance and counsel, and her friendship through the duration of this investigation. Special thanks are expressed to Dr. Grace Miller, Miss Mary Morr, Miss Doris Downs,» Miss Grace Masuda, Miss Zenovia Lukianchuk and Mrs. Beverley Wilkinson for their partici- pation on the taste panel. The author is thankful to Mr. Lyman J. Bratzler for his help in procuring and dissecting the beef rounds and to Dr. William Baten for his assistance with the interpretation of the statistical data of this study. *************** ii TABLE OF CONTENTS Page INTRODUCTION.................. ...... 1 REVIEWOFLITERATURE.................. 4 Factors Affecting Cooking Losses in Meat . . . . . . . 4 CompositionofMeat............... 4 Grade....................... 5 Weight and surface area . . . . . . . . . . . . . 6 Aging............. ...... 6 Cooking method, temperature, time . . . . . . . 7 Factors Affecting Rate of Heat Penetration. . . . . . . 9 Compositionofmeat............... 9 Weight and surface area . . . . . . . . . . . . . ll Aging.......................11 Initial temperature of the meat. . . . . . . . . . 11 Cooking method, temperature, time . . . . . . . 12 Factors Affecting Palatability Characteristics. . . . . 14 Aromaandflavor ..... .......... 14 Appearance and texture. . . . . . . . . . . . . . 16 Tenderness............... ..... 19 Juiciness..................... 24 Methods of Evaluating Palatability . . . . . . . . . . . 27 Subjective evaluation . . . . . . . . . . . . . . . 27 Objective evaluation. . . . . . . . . . . . . . . . 29 Combination subjective and objective evaluation . 30 METHOD OF PROCEDURE . . . . . . . . . . ........ 32 Procurement of Samples . . . . . . . . . . . . . . . . 32 EquipmentUsed..................... 33 Measuring and weighing. . . . . . . . . ..... 33 Roasting...................... 34 Slicing....................... 35 Objectivetesting................. 35 iii TABLE OF CONTENTS - Continued Page Preliminary Investigations ....... . . . . . . . . 35 Powerstat setting. . ....... . . . . . . . . 39 Thermocouple placement. . . . . . . . . . . . . 40 Preparation of samples for subjective testing . . 40 Preliminary taste panel . . . . . . . . . . . . . 42 Preparation of samples for objective testing . . 42 Preroasting Preparation . . . . . . . . . . . . . . . . 43 Preparation of raw roasts . . . . . . . . . . . . 43 Cooking temperature . . . . . . . . . . . . . . . 44 CookingProcess.................... 45 Conventional method . . . . . . . . . . . . . . . 45 Radial-conventional method . . . . . . . . . . . 45 Treatment After Roasting. . . . . . . . . . . . . . . . 45 Treatment of roasts before chilling . . . . . . . 46 Treatment of roasts after chilling . . . . . . . . 46 Evaluation of Samples. . . . . . . . . . . . . . . . . . 46 Subjective testing. . . . . . . . . . . . . . . . . 46 Objective testing . . . . . . . ..... . . . . . 47 Heatpenetration................. 48 Analysisofthedata................ 48 RESULTSANDDISCUSSION ................. 50 Palatability ........ . . . . . . . . . . . . . . . 50 Aromaandflavor. . . . . ..... . . . . . . . 51 Color and texture. ..... . . . . . . . . . . . 52 Juiciness..................... 54 Tenderness.................... 56 CookingLosses..................... 59 Total cooking losses . . . . . . . . . . . . . . . 61 Driplosses.................... 61 Volatile losses ..... . . . . . . . . . . . . . 61 Volume losses . . . . . . . . ..... . . . . . 62 pH....................... ..... 63 Heat Penetration . . . . . . . . . . . . . . . . . . . . 64 SUMMARY AND CONCLUSIONS. . . . . . . . . . . . . . . . 72 LITERATURECITED...... ..... 77 APPENDIX , , , 85 C O O O O O O C O 0 O O O O O O O O O 0 Q 0 0 iv TABLE 10. 11. LIST OF TABLES Average palatability scores for seven judges for six samples for two methods of cooking. . . . . . . . . Analyses of variance of aroma and flavor scores for twomethodsofcooking. . . . . . . . . . . . . . . Analyses of variance of color and texture scores for twomethodsofcooking. . . . . . . . . . . . . . . Average press fluid yields and grand average juici- ness scores 0 O O O O O O O O O O O O O O O O O O O 0 Analyses of variance of press fluid yields and juici- ness scores for two methods of cooking . . . . . . Grand average scores for softness, friability, residual tissue, and tenderness, and mean shear values for two methods of cooking . . . . . . . . . . Analyses of variance for softness, friability, residual tissue, and tenderness scores, and shear force values for two methods of cooking . . . . . . Correlation coefficients for all possible combi- nations of softness, friability, residual tissue, and tenderness scores, and Warner Bratzler shear measurements.................... Mean per cent cooking and volume losses of six replications for two methods of cookery . . . . . . Analyses of variance of cooking and volume losses for twomethodsofcookery. . . . . . . . . . . . . Mean percentages for volume loss, changes in linear measurements and total weight loss for two methodsofcookery. .. .. . . . . .. . . . .. V Page 51 52 54 55 55 56 58 58 60 6O 62 LIST OF TABLES - Continued TABLE 12. 13. 14. 15. l6. 17. 18. 19. Page Weight of rounds, muscle weight, maximum linear measurements of muscles, and trimmed sample weight......................... 86 Average palatability scores of seven judges for six replications for two cooking methods . . . . . . . . 87 Average press fluid yields and shear force values of cooked samples for six replications for two cook- ing methOdSO O O O O Q 0 O O O O O C O O O I O O O O O 88 Cooking weight losses for six roasts for two methods ofcookery...................... Volume loss for six roasts for two methods of cookery I I O O O C O C O I O I O O O O O O O O O O O O Determinations of pH for raw and cooked samples and the change in pH for six roasts for two methods Of COOking. O O O O O O O O O O O O O O O O O O C O O 0 Changes in the rate of heat penetration for five replications, for three sample depths for the radial- conventional cooking method. . . . . . . . . . . . . Changes in the rate of heat penetration for five repli- cations, for three sample depths for the conventional cookingmethod........ ............ vi 89 9O 91 92 93 LIST OF FIGURES FIGURE Page 1. Thermal rod and sample roast. . . . . . . . . . . . 36 2. Splitting the raw roast . . . . . . . . . . . . . . . . 36 3. Inserting the thermal rod . . . . . . . . . . . . . . 37 4. Tyingtherawroast................. 37 5. Positioning potentiometer leads: 3 = 0. 25-inch, b and _c_ = 0. 75-inch and 1.0 inch from surface; _d_ = attached to thermal rod . . . . . . . . . . . . . . . 38 6. Connecting thermal rod in auxiliary circuit; 2 = potentiometer lead for oven temperature record, 3: connection of rod to circuit . . . . . . . . . . . 38 7. Location of samples for objective and subjective evaluation of cooked roasts . . . . . . . . . . . . . 41 8. Average time-temperature relationships during cooking for three sample depths for two cooking methods....................... 65 9. Average rate of heat penetration: Min/0C rise for three sample depths for the radial-conventional cookingmethod................... 68 10. Average rate of heat penetration: Min/0C rise for three sample depths for the conventional cooking method 0 O O O O O O 0 0 O O O O O O O O O O O O O O O 69 vii IN TROD UC TION One of the primary concerns of all food service operators is the production and service of quality food at a price satisfactory to the consumer and commensurate with an adequate operational profit percentage. Because of the nutritional and satiety values of meat and the popularity it enjoys in the human diet, meat has become a daily necessity for every food service menu. Moreover, due to the cost of procurement and losses incurred in preparation and service, cuts of meat suitable for roasting represent a larger initial cost per sliceable portion than do cuts which are acceptable for menu items requiring cubed or ground meat. Cuts of meat are classified as tender and less tender, depend— ing upon the location in the carcass and the amount of exercise the muscles have received. In addition, varying degrees of tenderness result from inherent differences in the type and extent of connective tissue present. Less tender cuts have a greater proportion of collagen and/or elastin than do tender cuts and are usually considered less desirable for roasting, broiling, or pan-broiling. Studies have shown that collagen can be softened and made more tender by the application of heat in the presence of moisture. Consequently, less tender cuts of meat are usually cooked by moist heat methods such as braising, boiling, or steaming. If a food service operator could utilize less tender, less expensive cuts of beef for roasting, and produce products of acceptable yield and palatability which could be sold at a price satisfactory to both the consumer and the operation, the resultant mutual gains would be invaluable. Moreover, if economies in time required for roasting could be effected, more efficient use of available equipment could be made. The primary objective of this study was to investigate the effect of a combination of internal radial heating and conventional oven roast- ing on the yield and palatability of semitendinosus muscle of beef round. Matching muscles from paired rounds were conventionally roasted as a control. Internal radial heat was applied by means of a specially constructed thermal rod installed in a closed electrical circuit. The rod was horizontally positioned in the center of the meat, and heat was transmitted directly from the rod to the sample. With heat being simultaneously available from two sources, the thermal rod and the oven, it was anticipated that total cooking time might be shorter for the radial-conventional method than for the conventional method alone. If this was a valid premise, it was expected that the radial-conventional method might decrease drip and evaporation losses, and increase total yield. In addition, the inherent moisture of muscle tissue held within the cells requires both heat and time to effect evaporation. The increased total heat per unit of time reacting with moisture within the muscle tissue might permit sufficient softening of the collagen to create desirable tenderness in the product, Research has shown that heat transferred from the cooking medium to the product being cooked does not necessarily penetrate all foods of similar classification at the same rate. In beef, dif- ferences due to heredity, feed, exercise, and age may cause varia- tions in the type and amount of fat, muscle tissue, bone, connective tissue, and moisture present. A roast cut from one carcass may contain proportionately more or less of any of these elements than a similar roast from another carcass of the same grade. Tissue density, shape, and surface area of raw roasts of similar weight may also differ markedly. Moreover, it is conceivable that factors related to sample composition and physical structure may be more important determinants of cooking time required for a specified degree of done- ness than initial roast weight. For this reason, it was questioned whether the present system of minutes per pound is a valid means for estimating roasting time for meat. . The second objective of this study, therefore, was to examine the resultant heat penetration data and investigate the potential for using such data as a more accurate basis for predicting roasting time for the semitendinosus muscle of beef round. REVIEW OF LIT ERAT URE Factors Affecting Cooking Losses In Meat Cooking losses in meat include both drippings and volatile losses. Numerous studies have been conducted to determine factors which contribute to an increase or decrease in these cooking losses. Factors which are known to influence cooking losses are: composition of meat, grade, weight and surface area, aging, and cooking method, temperature and time. Composition of meat An early study conducted by Grindley and Mojonnier (35) showed that both water and fat accounted for the weight loss in roasted meats. Grindley, McCormack, and Porter (34) observed the major portion of weight loss in rrieat resulted from evaporation of water during cooking. Lowe (48) reported that the ratio of evaporation loss to dripping loss is higher for lean meat than for fat meat. The findings of Alexander (3) are in agreement with this statement. She found that dripping losses for rib roasts cooked in a 1250C oven to an internal temperature of 580C varied from 3. 7 per cent for the well-fattened Choice grade to 0. 4 per cent for the leaner Canner grade. Evaporation losses for these roasts ranged from 6. 5 per cent for the Choice grade to 10. 9 per cent for the Canner grade. Thille, Williamson, and Morgan (79) studied rib roasts cooked at 210°C to an internal temperature of 650C. Their data revealed greater total cooking losses for fat-surfaced roasts than for lean- surfaced roasts with volatile losses greater in the latter than in the former. According to Paul and Bratzler (62), steaks from semi- membranosus muscle, cooked in deep fat at 147°C to an internal temperature of 630C, had lower cooking losses than steaks from the adductor muscle cooked in an identical manner. Grade An increase in the grade of meat usually results from an in— crease in fat content. This being true, cuts from carcasses of higher grade usually have greater cooking losses (48). Two grades of sirloin butt were studied by Dunnigan (32). His results showed that the composition of meat had a direct influence on cooking weight losses, with leaner roasts yielding lower total cooking losses. Alexander and Clark (4) found that rib roasts from higher grade beef usually showed greater dripping losses and smaller volatile losses than similar roasts from lower grade beef. Masuda (54) re- ported no significant differences in volatile losses or total cooking losses attributable to grade, but average dripping losses at 900C internal temperature for Good and Choice grade roasts were signifi- cantly higher than those for Commercial grade roasts. No significant difference was found between the average dripping losses of Good and Choice grade roasts cooked to 90°C. In another study, Day (28) roasted longissimus dorsi muscles of Good, Commercial, and Utility grades of beef. Her data showed no significant differences attributable to grade in average total cooking losses, volatile losses, or drip losses. Choice and Good grade beef rounds were cooked to an internal temperature of 90°C by Aldrich and Lowe (2). They found high losses for all cuts, but noted no significant differences between grades . Weight and surface area McCance and Ship (55) cooked pieces of top round beef weighing 50, 400,. and 1500 grams each, at 100°C in steam. Regardless of sample size, total per cent losses of water were the same. The results of Cline e_t a1. (21) are in agreement with these findings. Marshall, Wood, and Patton (53) roasted 5-, 10-, and 15-pound cuts of Choice top round of beef. They found the size of the roast did affect the total per cent cooking losses. Losses for the 5-pound roasts were significantly greater than for the 10- and l5-pound roasts at all degrees of doneness except rare. Two studies (43,49) have shown that compact pieces of meat with smaller surface areas have less cooking weight losses than pieces of similar weight which have irregular shapes and greater surface areas. Thin, flat roasts, however, require a shorter cooking time per pound than thick, blocky roasts. 45233 Research conducted by Moran and Smith (57) using top round, bottom round, and loin roasts ripened 3, 7, and 16 days showed that with longer aging periods cooking losses decreased. A study con- ducted at Iowa State College (42) showed beef roasts with a longer ripening period exhibited lower weight losses when cooked in air than when cooked in steam, fat, or water. According to Alexander and Clark (5) the cooking losses and time required to cook lamb and mutton were decreased with increased ripening periods. Hanson gt a_L_l. (40) reported similar findings in their study of New York dressed broilers held at 1.7OC. Cooking method, temperature and time Lowe (48) states that the method of cooking influences not only the total but also the relative proportions of the different constituents lost during cooking. Clark and Van Duyne (18) compared cooking losses resulting from top round beef roasts cooked in a pressure saucepan with similar samples roasted in the oven to an internal temperature of 820C. They observed significantly greater losses in drip and total cooking losses in the roasts cooked in the pressure saucepan than in the oven roasts. Several investigators (21, 59) have reported that low internal temperatures of meat at the beginning of the cooking period result in increased cooking losses. However, Lowe and co-workers (49) found that cooking losses for frozen cuts of meat were not always greater than those of defrosted meat. Morgan and Nelson (58) reported that skewered meat yielded smaller and less consistent decreases in total shrinkage of meat than unskewered roasts. The total loss of weight in skewered roasts averaged 27. 3 per cent as compared with 31. 5 per cent for unskewered roasts. The results of other investigators support these findings (13, 21). Cooking losses of meats roasted in covered and uncovered pans have been compared by several investigators (35,46, 55). 7 Findings from these studies indicate that weight losses are greater when meats are roasted in covered pans than when cooked in open pans. The question of whether high cooking temperatures increase or decrease cooking losses has been investigated by many research workers. Lowe (48) states that, in general, when cooking temperature is increased cooking losses also increase. Child and Satorius (17) roasted semitendinosus beef. muscles at oven temperatures of 125, 150, 175, and 2000C to an internal temperature of 580C. They con- cluded that with increased oven temperatures rate of evaporation was accelerated and greater cooking losses resulted. A similar study (47) using standing rib roasts seared at 2500C for 20 minutes and cooked at temperatures ranging from 110 to 1750C, showed that average total cooking losses increased in proportion to rise in oven temperature. Total average cooking losses varied from 13. 52 per cent in roasts cooked at 110°C to 22.49 per cent in roasts cooked at 175°C. Using very low oven temperatures, Bramblett it a}. (10) found that beef'cooked at 630C had a lower percentage of cooking losses and a higher percentage of moisture content than beef cooked at 680C. The results of other studies (19. 20, 21) are in agreement with these findings. Lowe (48) states that if other conditions are standardized, the more well-done meat is cooked the greater the cooking losses. According to Child and Fogarty (16), who cooked semitendinosus muscles of beef round at an oven temperature of 150°C to internal temperatures of 58 and 750C, the higher the internal temperature was, the greater were the cooking losses. Earlier meat studies (35,47) also indicated an increase of cooking losses with an increase in degree of doneness. Aldrich and Lowe (2) cooked pot roasts from Choice and Good grade beef rounds, rump on, at an oven temperature of 150°C to an internal temperature of 900C. An additional hour of cooking after the internal temperature reached 900C increased the average total weight loss 38.9 per cent, the average volatile loss 27. 2 per cent, and the average volume loss 25. 3 per cent. In another investigation (79), rib roasts of beef cooked at 2100C . 0 . to an internal temperature of 65 C showed m01sture losses were directly proportional to the length of roasting time. Paul and Bratzler (62), in their study of steaks from semimembranosus and adductor muscles cooked in deep fat, found a high positive correlation between cooking time and cooking losses. Factors Affecting Rate of Heat Penetration The time required for cooking meat is often stated in minutes per pound. However, the extreme variability of meat coupled with the many interdependent factors influencing heat transfer make it difficult to forecast the exact time required for cooking. Major factors which influence cooking time required for meat will be reviewed under the headings of composition of meat, weight and surface area, aging, initial temperature of meat, and cooking method, temperature and time. Compo sition of meat Because of variances in thermal conductivity and heat capacity in all matter, the rate and extent of heating of any given material is dependent upon the nature of the product itself. Also, the rate of heating may be affected by the consistency and homogeneity of the product (30). Lowe (48) filled pint jars with lean beef, lean pork, fat pork, and suet to obtain data on the rate of heat penetration in muscular and fatty tissue. The four jars were placed in a processing container and heated in boiling water and steam for 3 hours. Temperatures were recorded by thermometers inserted through rubber corks in the lids of the jar and processor to the center of the jar. Data showed the rate of heat penetration was greatest in the lean beef and decreased in the order of lean pork, fat pork, and suet. Thille, Williamson and Morgan (79) studied the rate of heat penetration into balls of beef fat. 10 They observed that as the fat liquified upon heating, the heat pene- tration rate increased. They suggested that surface fat of meat tends to increase the rate of heat penetration due to its early melting in the cooking process, whereas, interior fat remains solid for a longer period of time and may retard the rate of heat penetration. Towson (80) cooked paired prime beef ribs at 125 and 200°C to an internal temperature of 630C. Temperatures were recorded at 4 points in each roast during cooking: (a) the center which was 2 inches from either cut surface of the longissimus dorsi muscle, (b) 0. 5 inch below the chine bone, (c) 0. 5 inch from the cut surface of the lean, and (d) 0. 5 inch below the surface of the fat over the top of the roast. Data showed that when the exterior fat layer was deeper than 0. 5 inch, so the thermometer bulb was embedded in the fat, the heat penetrated the 0.5 inch layer of fat more slowly than the 2 inches of lean. In their study of rate of heat penetration in roasts, Thille, Williamson, and Morgan (79) reported fat covered roasts required 23.4 minutes per pound as compared to 19. 3 minutes per pound for lean roasts. Cline and associates (21) studied six different kinds of roasts from 450 cuts of beef to determine how different methods of cooking affect the quality and palatability of beef. Roasts were cooked to an internal temperature of 570C in a 1250C oven. They reported that boneless roasts took longer to cook than cutswith bone. Prime rib roasts required 24 minutes per pound, unboned rump 23 minutes per pound, and two cuts from the chuck 18 minutes per pound. Sirloin tip roasts, and the heel of the round, with no bone, cooked in 33 and 29 minutes per pound respectively. Contrary to this, other investi- gators (79) have reported bone to be a poor conductor of heat. ll WeiQt and surface area Lowe (48) states that as the size of a piece of meat increases, its weight increases in greater ratio than its dimensions. This being true, if other conditions are standardized, larger cuts such as roasts and hams will require fewer minutes per pound for cooking than smaller similar cuts. 1 T0p round roasts weighing 5, 10, and 15 pounds were cooked by Mar shall, Wood, and Patton (53). Analyses of these data indicated that as size and degree of doneness increased, total cooking time also increased. However, fewer minutes per pound were required for cooking the larger roasts. Thille gt a_1. (79) reported a longer heating period per unit of weight was necessary for lean-surfaced roasts even though they were equal to or larger-than fat- surfaced roasts. {isles According to Alexander and Clark (5), from their work with mutton and lamb, increased ripening periods after slaughter shortened cooking time. Hanson e__t a_._l. (40) found that heat penetration was more rapid as post mortem changes in broilers progressed. Steaks from semimembranosus and adductor muscles were cooked in deep fat by Paul and Bratzler (62) to study the effect of lengthened storage periods. For the semimembranosus steaks, increased storage tended to increase cooking losses but decrease cooking time. In the adductor, steaks cooking losses were not altered significantly with in- creased storage, but cooking time was decreased slightly. Initial temperature of the meat A longer cooking time is required when the initial temperature of meat is low than when it is high. Meat still frozen at the beginning of a cooking period requires a longer cooking time because part of the 12 heat is being used to melt the ice before the temperature can be raised above the freezing temperature of the meat (48). The effect of four methods of defrosting meats and of the manner and temperature of cooking upon weight loss and palatability of roasts was studied by Lowe and associates (49). A longer cooking time was required for frozen cuts of meat than for comparable cuts which were thawed . Cookinginethod, temperature and time If the temperature of different cooking mediums is the same, time required for cooking depends to a great extent upon the rate of heat transfer in the particular medium being used (48). Harrison (42) reported that cooking time for beef was shortest in water followed by fat, steam, and air, respectively. Cover (22) compared the cooking times required for similar roasts cooked in a water bath and in an oven of the same temperature. The time required to reach the desired internal temperature was less than 3 hours in the water bath and more than 23 hours in the oven. Twelve top round beef roasts, weighing 5 pounds each, were cooked in the pressure saucepan or oven roasted to an internal temperature of 82°C by Clark and Van Duyne (18). Roasts cooked in the pressure saucepan required two-thirds less time than those cooked in the oven. According to a study by Visser e_t al. (82), roasts cooked in deep fat had shorter and steeper time-temperature curves than did comparable roasts cooked in the oven. At a given temperature, heat conductivity of liquid fat was approximately six times that of air. The use of skewers to reduce cooking time has been investigated by several workers (13, 23, 58). Morgan and Nelson (58) compared the cooking times required to reach a given interior temperature by skewered and unskewered two—rib standing beef roasts. Their findings 13 showed that the rate of cooking increased from 30 to 45 per cent when nickel-plated copper skewers were used. Similar results were found when Cover (23) cooked paired round, arm-bone chuck, and standing rib roasts of beef to an internal temperature of 800C in a 1250C oven, one with and the other without skewers. Skewers decreased both cooking time and cooking losses. Lowe (48) pointed out that the higher the cooking temperature, the more rapidly a piece of meat will reach a definite temperature, for, with a higher temperature at the surface, the more rapidly heat will penetrate into the interior of meat. In annearly experiment, Latzke (47) roasted standing ribs of beef to the same degree of doneness at different oven temperatures. She found the rate of heat penetration per pound of raw meat increased in proportion to the rise in external temperature. Cline, Loughead, and Schwartz (20) studied the effect of two roasting temperatures, 257 and 3110F, on the palatability of beef roasts. In all cases, the time per pound required for roasting was lessened by the higher oven temperature. Cooking meat rare requires a shorter time than cooking it medium or well done if all other conditions are standardized (48). Latzke (47) found that roasts cooked in a 125°C oven to different degrees of doneness required varying average minutes per pound. The rare beef roasts required 14.19 minutes, the medium 16.44 minutes, and the well-done 22, 91 minutes. According to a recent study by Marshall gt a}. (53), roasts cooked to 70 and 80°C in a 93°C oven exhibited wide differences in rate of heat penetration. Roasts cooked to these same internal temperatures at higher oven temperatures, 107 and 121°C, showed less variation. Roasts cooked to 600C showed the most uniform rates of heat penetration among oven temperatures used in the experiment, 14 Factors Affecting Palatability Characteristics "Regardless of the nutritional excellence and adaptability of meat and meat products as an item of the diet, meat will be con- sumed in adequate and increasing quantities only if it appeals to and is accepted by the consumer on the basis of its palatability or taste characteristics (84). " The palatability of cooked meat depends upon six major qualities: aroma, flavor, appearance, texture, tenderness, and juiciness. These, in turn, are affected by one or more of the following factors: composition of the meat, grade, aging, and method and extent of cooking. Aroma and flavor Meat flavor and aroma are difficult to separate because many of the flavor properties are the result of odor sensations. Raw meat flavor is weak, salty, and blood-like. True meaty flavor is developed during cooking and is thought to arise from chemical changes in the muscle fiber protein. Many of the flavor components of cooked meat are water-soluble, but some may be found in the water-insoluble nitrogenous fraction from muscle fibers (84). Grade. The effect of carcass grade on aroma and flavor has been reported by numerous investigators. In comparing longissimus dorsi muscles of Good, Commercial, and Utility grades, Day (28) reported significant differences in flavor and aroma of the cooked meat due to grade. Griswold (36) studied the effect of different methods of cooking beef round of Commercial and Prime grades. Large dif- ferences were found between animals within a grade but, on the whole, palatability scores were higher for beef rounds of Prime than of Commercial grade. Statistical analysis conducted by Masuda (54) 15 showed the average aroma and flavor scores from Commercial grade beef to be significantly higher than those from Good and Choice grade roasts. Lowe e_t 31' (49) compared Choice, Good, and Commercial grade rib roasts. Highest scores for aroma and flavor were obtained from the Choice grade. In her study of the palatability of two grades of sirloin butts, Dunnigan (32) cooked Choice and Utility grade sirloin butts, with and without bone, at an oven temperature of 150°C to an internal tempera- ture of 70°C. Scores for aroma and flavor were significantly higher for Choice roasts than for Utility roasts. Barbella and associates (6) cooked 728 beef rib roasts to study the effect of fatness and other factors on flavor and juiciness of beef. They observed that the lean meat from wellwfed animals was very superior in flavor to meat from poorly-fed animals. Other investigators (11, 76) have reported that with increased degree of finish there is an increase in flavor scores. Aging. Cooked unaged beef is quite metallic and astringent and lacks typical beef flavor. True beef flavor is fully developed by approximately 8 days' aging; as the beef becomes more tender during aging, the bouquet develops (84). The effect of storage conditions on the palatability of beef was investigated by Paul, Lowe, and McClurg (64). Paired rounds of Good grade steer were stored and checked at intervals up to 18 days. The flavor and aroma improved up to the 9th day, but thereafter scores for these characteristics decreased with increased aging of the meat. These decreases were attributed to the development of gameness in the lean, and rancidity in the fat. A similar study by Griswold and Wharton (37) reported that meat aged 37 days had somewhat stronger aroma and flavor than meat aged nine days. 16 Harrison (41) studied the histological, physical, and organo— leptic changes in beef during the ripening period. She found that at temperatures of 34 and 36°F, the first ten days of ripening yielded the greatest improvement in palatability. A musty odor and a high flavor developed between 20 and 30 days of aging. Method and extent of cooking. Twelve comparable top round beef roasts were cooked in the pressure saucepan or roasted in the oven to an internal temperature of 82°C by Clark and Van Duyne (18). Results indicated that more palatable meat was obtained in the oven than in the pressure saucepan, Judges preferred the flavor of the lean and fat of the oven roasted meat. Cline and associates (21) reported lower flavor and aroma scores for tender beef cuts cooked with added water in a 125°C oven than for comparable oven roasted cuts. Griswold (36), in comparing the effect of different cooking methods, found beef round roasted at 121°C scored high in flavor and acceptability. Similar findings were reported by Stech and West (77). Latzke (47) seared standing rib roasts at 250°C for 20 minutes and then cooked them at various temperatures from 110 to 175°C. The medium-done roasts cooked at an oven temperature of 125°C gave a product with maximum desirability of flavor, Aldrich and Lowe (2) found that an additional hour of cooking at 150°C, after the internal temperature of beef round pot roasts reached 90°C, decreased the scores for aroma and flavor. According to Weir (84), an increase in the sulfury flavor component results from this additional holding period. Appearance and texture Crist and Seaton (26) reported that appearance ranked first in importance according to their judges' scores . Brady (9) concluded from his study of factors influencing tenderness and texture of beef, 17 that texture is dependent upon the size of the bundle of muscle fibers, and the greater the number of fibers in a bundle, the finer the texture. This conclusion is substantiated in part by the fact that longissimus dorsi muscles which are considered to have good texture have been shown to have more fibers of a similar diameter per primary bundle than do muscles of less desirable texture such as the semitendinosus (7). 223513. According to Masuda (54), the average appearance scores for roasts of Commercial and Good grades were significantly higher than for roasts of Choice grade. No significant differences in texture were found attributable to grade. Day (28) reported that judges found little difference between grades in appearance or texture. An earlier study by Satorius and Child (72) showed no significant difference between medium and good grades in the external appearance of longissimus dorsi and adductor muscles cooked to 58°C at a 150°C oven temperature. However, a significant difference was found between the two grades in the external appearance of the raw muscle. 2919;. According to Weir (84) the color of cooked, uncured, lean meats depends to a great extent upon the nature and amount of myoglobin derivatives and decomposition products present. Pigment changes that occur during cooking also help determine the final color of cooked meats; these changes being determined by the type, length, and temperature of cooking. Visual color changes occurring in meat are related to temperature as follows: below 60°C, little or no color change; 65-700C, decreasing pinkness to 70°C; at 759C, complete loss of pinkness. The fat portion of meat changes very little in color during cook- ing except for surface browning which increases the attractive appearance of meat. Fat decomposition and poly'rnerization with 18 carbohydrate and protein decomposition products cause this surface browning. The greenish iridescence on the surface of cut cooked meat is caused by the refraction of light in the thin layer of fat spread over 1 its surface by slicing (84). Methpd and extent of cooking: Winegarden e_t a_._l. (86) studied the physical changes occurring in connective tissue of beef during heating. Results showed that strips of connective tissue softened, shortened in length, decreased in width, and increased in thickness during heating. These changes occurred together, and as cooking time increased and temperature of heating was elevated, the degree of changes increased. Bramblett and associates (1‘0) cooked five muscles from six pairs of Standard grade beef rounds, one of each pair at 630C for 30 hours, and the other at 680C for 18 hours. Taste panel scores for texture and appearance were higher for meat cooked at the lower temperature than for meat cooked at the higher temperature. In studying the effect of extent of cooking on palatability and edible portion of Good and Choice beef rounds, Aldrich (1) found that cooking for an additional hour after the internal temperature reached 900C resulted in lower average appearance scores than cooking only to 900C. Data from a similar study (53) showed that appearance tended to be scored lower as the degree of doneness increased. Lukianchuk (51) compared the effect of two methods of dry heat cookery on palatability and cooking losses of paired semimembranosus muscles of beef round. Taste panel members scored the texture of conventionally roasted samples slightly higher than those cooked in deep fat, although statistical analysis of these data indicated no significant difference in texture attributable to cooking method. An early study by Morgan and Nelson (58) revealed that meat from unskewered roasts was drier and less appetizing in appearance than meat from skewered roasts. Roasts cooked well-done in covered pans 19 did not differ in interior appearance from roasts similarly cooked in uncovered pans . Tenderness The most important palatability factor in the acceptance of beef, as determined by consumer studies, is tenderness (84). Many factors both ante-mortem and post-mortem, influence the tenderness of beef. Composition of meat. Lowe (48) states that collagen affects the tenderness of meat to a greater extent than elastin because the ratio of elastin to collagen is small. Collagen is converted, to gelatin by heat in the presence of water, whereas, little change occurs in elastin. The effect of intramuscular fat on tenderness of meat has been studied by many investigators. Helser, Nelson and Lowe (43) reported that betters-fattened animals yield more tender meat than less-fattened ones. Contrary to this, Hankins and Ellis (39) reported no significant cor relation between the fat content and tenderness of cooked longissimus dorsi muscles. They concluded that variations in tenderness are caused mainly by factors other than fat content. Instudying the comparative tenderness of samples from eight muscles of Good grade beef, Ramsbottom and Strandine (70) found no relationship between the amount of fat within the muscle and shear force values of raw or cooked samples. They concluded that the relative amounts of collagenous and elastic connective tissue in the muscle in— fluenced the tenderness of the cooked muscle. Age, sex, and grade. Brady (9) believes that the texture of meat is an indication of tenderness, and the finer the texture, the more tender the meat. His data demonstrated that with an increase in the age of the animal there is an increase in the diameter of muscle fibers. ~ A mean diameter of 58. 8 microns was reported for the same 20 muscle in yearling steers as compared with 70. 9 microns for mature cows. Hiner and Hankins (44) studied animals ranging in age from 2.5 to 5.5 years to determine the effect of animal age on tenderness of meat. Increases in animal age were accompanied by decreases in tenderness. The findings of MacKintosh and co-workers (52) sub- stantiate these results. Satorius and Child (74) roasted cuts from six steers graded high- medium to good and from seven cows graded good, to an internal temperature of 58°C in a 150°C oven. Tenderness measurements by the Minnesota modification of the Warner Bratzler shear apparatus showed that meat from steers was much more tender than meat from cows. Shear force required for steers was 18 pounds as compared to 28 pounds for cows. Day (28) reported differences in tenderness attributable to grade, significant at the 1% probability level, for longissimus dorsi muscles of Good, Commercial and Utility grades of beef. Average tenderness scores for roasts from tender cuts of Commercial, Good, and Choice grade beef cooked by Masuda (54) revealed that Choice grade roasts were significantly more tender than Commercial and Good grade roasts. No significant difference in tenderness was found between the average scores of Commercial and Good grade roasts. Animal and muscle variations, Weir (84) states that among muscles in any one animal there is a wide variation in tenderness. In general, muscles containing the largest amount of connective tissue are the least tender, and those with the smallest amount are the most tender. Noble, Halliday, and Klaas (60) investigated the tenderness of cooked beef by means of a slightly modified New York Testing Labora- tory Penetrometer. With one exception, rib cuts of beef were one 21 and one-half times more tender than first round cuts from the same animal. Ramsbottom and Strandine (70) studied 25 representative beef muscles from three commercially graded Good carcasses cooked in 121, 1°C lard to an internal temperature of 76.70C. Shear tests with the Warner Bratzler machine showed a significant variation in tenderness from muscle to muscle. Bramblett and associates (10) reported significant differences in shear values among five muscles of Standard grade beef round cooked by two different methods. In her study, Griswold (36) found no significant differences between shear values of semimembranosus and biceps femoris muscles of beef round. Contrary to this, Mitchell (56) concluded that the inner round is more tender than the outer round. Examination of 12 rounds indicated that inside muscles contained much smaller percentages of elastin and collagen than outside muscles. Aging. Lowe (48) reported that Lehmann was one of the first persons to measure the increase of tenderness of beef during aging. In his work with flank and loin muscles, he showed that aging increased tenderness. When meat is held at 0.50C after slaughter, there is a decrease in tenderness during the first 24 hours while rigor mortis sets in, and then a gradual increase in tenderness (84). In 1944, Paul at a1. (64) studied the changes in histological struc- ture and palatability of beef during 1. 70C storage over a 4-day period. They observed that the greatest increase in tenderness of small beef cuts coincided with their observed increase in frequency of fiber breakdown. According to Moran and Smith (57), alteration in the protein of muscle fiber is the most important change during the first few days after death during the disappearance of rigor. Several days after death rigor is completely resolved and any further increase in tenderness is due mainly to the softening and swelling of the collagen. In contrast 22 to this Wierbicki e_t a_L_1. (85) believe that connective tissue changes cannot account for the tenderness produced by aging. From their experiments, Birkner and Auerbach (7) concluded that aging has no apparent effect upon elastic fibers. Freezing. Regarding the tenderizing of beef by freezing, there is no unanimity of opinion. In their work with steaks, Pearson and Miller (65) found freezing by itself did not increase tenderness, whereas frozen storage brought about a decrease in tenderness. Ramsbottom (69) reported that frozen storage at —23.30C or lower for as long as 7 years did not significantly affect the tenderness of beef steaks. Paul and Child (63) observed no significant difference in the tenderness of meat which was frozen at —180C and meat which was not frozen. Hiner and Hankins (44) studied paired short loins of beef and observed that freezing had more tenderizing effect than storage at 34°F. They reported that beef frozen at 20°F was approximately 12 per cent more tender and beef frozen at ~10 or -40°F was 18 per cent more tender than beef ripened at 34°F. No significant difference in the tenderizing effect was found between loins frozen at -40°F and those frozen at ~100F, Method and extent of cooking; Cover (23) studied the effect of metal skewers on cooking time and tenderness of beef. She found that the use of skewers in paired round-bone chuck roasts decreased the tenderness of the cooked product. Conversely, Morgan (58) found that skewered roasts were more tender than those cooked without skewers. Lukianchuk (51) studied semimembranosus muscles of beef and reported no significant differences attributable to cooking method for softness, friability, residual tissue, general tenderness, or shear force. 23 In another study, Clark and co-workers (18) compared the effect of oven roasting and pressure saucepan cookery on palatability of beef muscles. No differences were detected by a mechanical shear stress apparatus or by subjective evaluation of tenderness in semimembranosus and adductor muscles of beef round cooked by either method. Cooking time and tenderness of meat cooked in a water bath and in an oven of the same temperature were compared by Cover (22). Both subjective and objective evaluations indicated roasts cooked in the oven were more tender than roasts cooked in a water bath. She con- cluded that "moist heat" in the sense of added moisture is not necessary for making tough meat tender. The results reported by Griswold (36) are in agreement with these findings. Lowe (48) pointed out that cooking time rather than cooking temperature is the factor which determines tenderness in meat. Child and Satorius (17) studied the effect of various oven temperatures on the tenderness of roasted beef muscles. Semitendinosus muscles heated to an internal temperature of 58°C at oven temperatures of 125, 150, 175, and 200°C did not differ in shear force values. Bramblett it a_._1, (10) reported beef cooked at 63°C as compared with beef cooked at 68°C was more tender. In another study, Cover (25) investigated the relationship between oven temperature and meat tenderness, Constant oven temperatures of 125 and 225°C were used to roast meat to an internal temperature of 800C. These data revealed roundw-bone chuck and rump roasts of beef, and half ham roasts of pork were more tender when cooked at 125°C than when cooked at 225°C. Cover concluded that the tenderizing effect noted in samples roasted at the lower temperature probably did not result from oven temperature alone, but from the combined effect of longer cooking period and lower cooking temperature. 24 According to a more recent statement by Weir (84), two changes occur during the cooking of meat: the muscle fibers become tougher, and the connective tissue becomes more tender. Although these two occurrences are dependent upon both time and temperature, the time factor is more important for collagen softening while the temperature factor appears to be more important for muscle fiber toughening. In studying the effect of cooking time on pot roasts from beef round, Aldrich and Lows (2) reported that an additional hour of cook- ing after the internal temperature of the roasts reached 90°C resulted in a slight increase in tenderness as shown by panel scores and shear force readings. Satorius and Child (72) studied the effect of coagulation at three stages of doneness on shear force values. They reported that with increased internal temperature semitendinosus beef muscles become more tender until 75°C was reached when the meat then was found to be less tender than that cooked to 67°C. They conclude that during the first two stages of coagulation, to 58°C and to 67°C, the effect of hydrolysis is evidently greater than that of increased density from coagulation; while in the last stage, to 75°C, the reverse is true. Noble, Halliday and Klaas (60) concluded from penetrometer readings that toughening occurred during heating from 61 to 75°C. Juicine ss Juiciness of cooked meat may be separated into two effects: the first is the impression of wetness during the first chews produced by the rapid release of meat fluids; the second is one of sustained juici- ness apparently due to the slow release of serum and to the stimulating effect of fat on salivary flow, 25 Composition of meat. Noble, Halliday, and Klaas (60) reported that beef rounds yielded more juice than standing rib roasts when both cuts were cooked to 61°C at an oven temperature of 149°C. Harrison (42) reported that, of the muscles included in her study, roasts from the psoas major muscle were most juicy and those from the semi- tendinosus muscle were the least juicy. Variation in juiciness from muscle to muscle was also found by Paul, Lowe, and McClurg (64), Using a pressometer to measure the press fluid in standing and rolled rib roasts of beef, Child and Esteros (15) concluded that standing rib roasts contained a larger quantity of juice than the rolled rib roasts. The findings of Alexander and Clark (4) are in agreement with this con- clusion. Well-marbled meat from mature animals with a relatively high degree of finish is juicier than that from young animals with less marbling (84). Stech and West (77) studied differences in eating quality factors of beef from 18- and 30—month old steers. Although age dif- ference did not affect juiciness significantly, anterior locations of semimembranosus, adductor, and longissimus dorsi muscles scored higher for juiciness than posterior locations. Thille, Williamson and Morgan (79) cooked beef rib roasts at 210°C to an internal temperature of 65°C, Their data showed that fat- surfaced beef roasts were less dry than lean-surfaced roasts, Barbella e1: a_1_l. (6) used 728 rib roasts to study the relationships of flavor and juiciness to fatness and other factors. They observed a rapid increase in juiciness with increased fatness up to 22. 5 per cent and more slowly thereafter to 42. 5 per cent. After a 42. 5 per cent fatness level was reached, there was no increased juiciness attribut- able to fatness . Grade. Several investigators (1, 28, 54) have reported findings on the relationship between carcass grade and juiciness. Masuda (54), 26 studying Choice, Good, and Commercial grades of beef, found no significant differences in juiciness attributable to grade. Day (28), using beef of Good, Commercial, and Utility grades, came to the same conclusion. According to Aldrich (l), the amount of press fluid found in Choice rounds was slightly higher than that found in Good rounds. Contrary to this, Vail and O'Neill (81) reported cooked Choice grade rolled rib, round, and clod cuts of beef yielded appreciably less press fluid than similar Good grade cuts. Aging. Changes which are noticeable during aging, according to Lowe (48), are increased tenderness, change in flavor, and increase in case with which juice may be pressed from the meat. Weir (84) states that tenderness and juiciness are closely related; the more tender the meat, the more quickly the juices are released by chewing, and the more juicy the meat appears. Harrison (41) reported little variation in juiciness scores of roasts stored 1 to 20 days. Juiciness scores decreased after 30 days of aging. She concluded that evaporation of the fluids in the raw roasts during storage was great enough to affect the juiciness scores of the cooked roasts. According to Paul (61), during the first few days of storage there is a reduction in press fluid, followed by a sharp in- crease between the 9th and 18th day of ripening. Method and extent of cooking. Morgan and Nelson (58) reported skewered roasts were more juicy than unskewered roasts when cooked at the same oven temperature and to the same internal temperature. Cover's (23) findings were in agreement with this. Cline and co-workers (21) used 450 cuts of beef to study the effect of cooking methods on palatability of meat. They reported that searing does not increase the juiciness of cooked meat. Clark and Van Duyne (18) reported that meat cooked in the pressure saucepan to an internal 27 temperature of 80°C was considered too dry by the judges. The results of Lukianchuk's study (51) showed that for both subjective and objective evaluations, conventionally roasted samples were juicier than those cooked in deep fat. Child and Satorius (17) studied the effect of exterior temperature on the juiciness of beef. They concluded that external temperatures do not affect either the amount of press fluid or the juiciness scores of comparable cuts of meat cooked to the same internal temperature. Contrary to these findings, Cline e_t 3.1: (21) reported that high oven temperatures decrease the juiciness of roasts. In their study, Bramblett e_t a}. (10) noted that meat cooked at 68°C for 18 hours yielded less press fluid and lower scores for juiciness than meat cooked at 630C for 30 hours. Aldrich and Lowe (2) found that an additional hour of cooking after the internal temperature of pot roasts reached 90°C decreased the juiciness of the product. In a 1938 study, Satorius and Child (74) reported that semitendinosus beef muscle, roasted to an internal temperature of 67°C, had more press fluid than comparable cuts roasted to 75°C. This difference was not evident when internal temperatures were increased from 58 to 670C. Noble, Halliday, and Klaas (60) also found that cooking to a lower internal temperature (61°C) yielded juicier meat than cooking to a higher internal tempera- ture (750C). Methods of Evaluating Palatability Sulljective evaluation Lowe (48) states that the acceptance or rejection of a food is based largely on the stimulus given the sense organs of an individual. Sight, smell, taste, and touch are the more important senses in food evaluation. 28 According to Weir (83), taste panels are of two main types, (a) the specialized research panel designed to determine differences in treatments and the magnitude of the difference of preference; (b) the consumer panel designed to indicate a preference and/or the degree of preference for the samples being evaluated. There are many types of sensory tests given to panel members. These may be ranking tests, scoring tests, triangle tests, paired sample tests, and others (48). Numerous investigators (24, 27,48,75) have described the procedure and discussed the advantages and disad- vantages of one or more of these methods of evaluation. Lowe (48) states that none of the methods for determining sensory differences is entirely satisfactory because: (a) with some methods, no evaluation of the particular gradation of quality is obtained, (b) all tests are not applicable to all products, and (c) evaluations may be compara- tive rather than absolute. According to Boggs and Hanson (8), the following factors affect the accuracy of results: (a) characteristics evaluated, (b) cooking of the sample, (c) quality of the foods, (d) reference standards, and (e) replications. Weir (83) suggests that the following points be remembered: (a) when sampling meat for tenderness, judges should be given a sample from the same relative position each time, (b) the temperature of the meat at the time of testing should always be the same, (c) samples should be large enough to allow judges to recheck the samples if they wish. Dove (31) recommends air conditioned rooms for testing to prevent odors of paint, coffee, smoke or other aromas from influencing judges' decisions. According to Peret (66) china, silver and glass utensils should be used in testing to assure optimum results. Other investigators (33) stress the importance of isolating judges, one from another, to assure accurate results. 29 At a symposium on Accepted Testing Methodology, held in Chicago in 1953 (67), Dr. H. Schlosberg, Brown University, reported that the best or only way to obtain more stable and sensitive measure- ments of response is to increase the size of the panel. The problem of planning organoleptic tests to increase the validity and accuracy of panel scores has been discussed by Crist and Seaton (26), Lowe and Stewart (50), and Dove (31). Objective evaluation Meat quality is determined by the total effect of its physical and chemical characteristics. Since certain organoleptic properties of meat are obviously dependent upon physical characteristics, attempts have been made to measure some physical characteristics objectively. Tenderness. Several mechanical devices have been developed to determine values related to tenderness. These instruments, the Warner-Bratzler shear machine, the recording strain-gauge denture tenderometer, the New York Testing Laboratory penetrometer, and others, have been described by Bratzler, Warner, and MacKintosh (12), Winkler (87) and Proctor gt a_1. (68). In general, either the force required to cut or shear through a piece of meat, or the amount of work done in cutting or shearing through a piece of meat, is measured by these instruments. A review by Schultz (75) outlines the advantages and disadvantages of some of the instruments and methods. Hurwicz and Tischer (45) studied the problems encountered with the Warner-Bratzler shear machine and pointed out some of the inherent errors. According to Doty (29), the following statements can be made concerning methods for evaluating objective tenderness: (a) samples to be tested must be of uniform size, (b) testing must be done at a single temperature, (c) several replicate tests must be run on each 30 sample, and (d) results obtained on raw beef are of little value as an indication of tenderness of cooked meat. Doty further states that if these general precautions are observed, shear or other objective tenderness measurements on cooked meat may correlate quite well with taste panel evaluations of tenderness. Several investigators (2, 73) have found a high degree of correlation between taste panel tenderness scores and shear force values. Juiciness. Since the liberation of meat fluids during chewing is responsible for the sensation of juiciness, an objective evaluation of juiciness can be determined by an estimate of the expres sable fluid from meat (29). In 1934, Child and Baldelli (14) designed equipment to determine the fluid expressed from cooked meat under 200 p. s.i. This they called a pressometer. In 1943, Tanner, Clark, and Hankins (78) adapted an hydraulic press to determine the fluid expressed from cooked meat at 2500 p. s.i. Doty (29) pointed out that for some types of cooked meats a fair index of organoleptic juiciness is obtained by these methods, but because juiciness is apparently due to the fat concentration and dis- tribution, this is generally not so. Child and Esteros (15) and Day (28) reported significant correlation between taste panel juiciness scores and pressometer readings. Aldrich (1) found good correlation between the percentage of press fluid determined by a pressometer and judges' scores for juiciness in muscles from Good and Choice grade beef rounds cooked to 900C. Tanner e_t a_l. (78) found no corre- lation between juiciness scores and hydraulic press fluid values. Combination of subjective and objective methods Due to the shortcomings of both subjective and objective tests when used separately, many workers have used a combination of the two methods for determining food acceptability. 31 Halliday (38) recommended the use of a combination of subjective and objective methods for food testing. The same recommendation was made by Dove (31) who stated that ". . . in order to judge the merit and range of use of each and every objective test, we must hunt out a scientific basis for our judgment by developing a method of measuring the subjective responses. " Other investigators (50) believe that objective tests for organoleptic qualities must measure those characteristics which are correlated with acceptability. METHOD OF PROCEDURE To compare the effect of two methods of dry heat cookery on palatability, cooking losses, and rate of cooking, twelve semi- tendinosus muscles from six pairs of U. 5. Choice grade steer beef rounds, rump on, were used. One muscle from each pair was con- ventionally roasted whereas the matching muscle was cooked by a combination of internal radial heating and conventional roasting. Procedures used for the preparation, roasting, and subjective and objective evaluations of the samples were developed through pre- liminary investigations in the laboratory. Procurement of Samples Paired steer rounds, weighing approximately 70 to 80 pounds per round, were purchased from the Farmer Peet Packing Company and delivered to the Michigan State” University animal husbandry meat laboratory. Six days after slaughter, the semitendinosus muscles were dissected from the rounds, the surface fat removed, and the muscles trimmed to provide roasts as comparable as possible in shape within a weight range of 1300 to 1500 grams. Trimmed samples were individually wrapped in Tite freezer paper, assigned code numbers from a table of random numbers, marked for identification of the anterior and posterior ends, blast frozen and stored at ~20°C until defrosted prior to cooking. Storage periods for the samples ranged from 91 to 105 days. Information regarding weight of rounds, weight of muscles, muscle linear measurements, and trimmed sample weight is given in Table 12, the Appendix. 32 33 Equipment Used With the exception of the thermal rod assembly, standard institutional roasting equipment was used throughout the study. In addition, certain pieces of special laboratory equipment were used to facilitate accurate collection of data. Measuringfiand weighing The device used for measuring the length, width, and depth of the raw and cooked test samples consisted of two vertical 18-inch rulers which were numbered from the bottom to the top. Connecting the two vertical rulers was a horizontal 24-inch ruler with numbers reading from left to right. The left vertical ruler was secured on a metal foot and could stand alone; the horizontal ruler could be moved up and down and the right vertical ruler could be moved toward or away from the left ruler. To determine length, the sample was placed between the verti- cal rulers and the right vertical ruler was adjusted so that the rulers touched both ends of the sample at the longest point. Anterior, middle, posterior, and maximum measurements were taken for the width and depth of each sample. To determine width, the sample was turned so that the vertical rulers touched both sides at the desired locations. To measure sample depth, the horizontal ruler was lowered until it touched the sample at the designated locations and leveled to the same inch marking of both vertical rulers. Length and width measurements were read from the horizontal ruler, whereas depth measurements were read from the vertical rulers. All linear measurements were recorded to the nearest 0.125-inch. I A large Pyrex cylinder, two lOOO-milliliter graduate cylinders, a rubber hose, a clamp, and a lZ-inch thermometer with a temperature 0 _ range of -20 to 100 C were used for volume measurements. 34 A torsion balance, with a capacity of 4. 5 kilograms, was used to determine the weights of the raw and cooked samples, the roasting pans, racks, and drippings. Readings were recorded to the nearest gram. An 8-point Minneapolis Honeywell Electronik Multipoint Recorder, Type 153, with a chart drive of 10 inches per hour, was used to record data relevant to internal oven temperature, thermal rod temperature during the cooking period, and time-temperature relationships of the roasts throughout the cooking and cooling periods. Roasting The thermal rod used for the internal heating of roasts was designed by and procured through the Food Service Industry Research Center at Michigan State University. The unit consisted of an electric wire mounted in a hollow, unpolished, stainless steel rod 12 inches in length and 0. 125-inch in diameter. The wire extended 6 inches beyond either end of the rod and was terminated with small clamps used for inserting the rod in an auxiliary electric circuit controlled by a power- stat. For this study, the raw meat was split lengthwise to a depth equal to the radius of the sample, the thermal rod horizontally inserted, and the roast tied securely with heavy cotton cord. The sample was placed on racks in a roasting pan, with the split in a lateral position, and the entire assembly placed in a preheated oven. The clamps of the thermal unit were connected to the auxiliary circuit and, when current was applied, electrical energy transmitted as heat energy penetrated the sample. The thermal rod used in this research and the consecutive steps in the procedure for splitting the raw sample, inserting the rod, tying 35 the sample, positioning the sample, and connecting the rod in the auxiliary circuit are illustrated in Figures 1 through 6. Standard aluminum roasting pans, 17. 25 inches long, 11. 25 inches wide, and 2.25 inches deep, were used for both cooking methods. Since single unit wire racks to fit these dimensions were not available, each pan was equipped with two overlapping 10. 5 inch square wire racks supported by 0. 5 inch legs. The lower deck of a 2-deck thermostatically controlled Hotpoint roasting and baking oven, Model No. HJ. 225 with an air cushion bottom, was used throughout the study. Special fabrication included interior lighting and two single-pane glass windows. Slicing A Hobart electric slicer, Model No. 410 positioned at no. 12 setting, was used to obtain slices 0. 25-inch thick for the taste panel evaluation. Objective te sting Three pieces of standard objective measuring equipment were used in this study. A Beckman zeromatic pH meter, Model No. 9600 with glass electrode, was used to determine the pH of both raw and cooked samples. A Warner Bratzler shear apparatus and a Carver laboratory press were used to evaluate tenderness and juiciness of the cooked samples, respectively. Preliminar y Inve stigations To determine procedures for the conventional and radial- conventional roasting methods, twelve crosscuts of U. S. Choice grade beef round, similar in weight and shape to the actual test samples, Figure 1. Figure 2. 36 Splitting the raw roast. Figure 3. Inserting the thermal rod. Figure 4. Tying the raw roast. 38 Figure 5. Positioning potentiometer leads: _a_ = 0.25-inch, b and g = 0.75 and 1. 0-inch from surface; _d_ = attached to thermal rod. Figure 6. Connecting thermal rod in auxiliary circuit: 3 = potentiome- ter lead for oven temperature record, _f_ = connection of rod to circuit. 39 were cooked and evaluated. Data from preliminary trials were used to establish the powerstat setting for thermal control of the rod and thermocouple placement for the time-temperature records during roasting. Methodology for the preparation of samples for subjective and objective testing was developed. Samples from the roasts were used for preliminary taste panel instruction. Power stat s etting Dial settings of 20, 30, 40 per cent, and a combination of 20 and 30 per cent were experimented with to determine a powerstat setting which would provide the maximum rod voltage which could be used without charring the adjacent muscle tissue. In this study power- stat settings of 20, 30, and 40 per cent reduced 110 volt current to 22, 33, and 44 volts, respectively. In each trial, the deck oven was preheated to- and maintained at 1490C during roasting, with the upper and lower grids set on medium and the damper half closed. When a powerstat setting of 30 or 40 per cent was used the inner tissue became charred during roasting and moisture loss was excessive. Experimentation with an initial setting of 30 per cent during the first half of the roasting period and a reduced setting of 20 per cent for the remainder of the roasting period produced similar results. Further work in which a setting of 20 per cent was used throughout the roasting periodgave a product in which the inner tissues were not discolored and moisture loss was comparable to conventionally roasted samples. From these data is was decided that a constant power stat setting of 20 per cent was most suitable for the radial-conventional roasting method. 40 The rmocouple plac em ent Conventional roasting. During preliminary study, one thermo- couple was positioned at a depth equal to the sample radius (1. 5 inches) for determination of the end cooking temperature for con- trolled doneness. A second thermocouple was mounted in the oven to record internal oven temperatures throughout the roasting period. Since rate of heat penetration in raw and coagulated tissue may differ, simultaneous continuous records of time—temperature relationships for different depths of sample tissue seemed desirable. ~ For collection of these data, additional thermocouples were positioned at 0.75 and 0.25 inches from the sample surface. Radial-conventional roasting: Experimentation with thermo- m couple placement for this method required consideration of the depth of tissue between the two heat sources. Mathematical calculations of the distance to be penetrated before heat from the two sources met, placed the contact point at approximately 1.0 inch from the surface. Accordingly, one thermocouple was used to determine end cooking temperature for controlled doneness at the estimated sample radius, a second to record the temperature of the thermal rod during roast- ing, and a third to record internal oven temperature during roasting. Two additional thermocouples were positioned at depths of 0.75 and 0.25 inches from the sample surface to collect data basic to the determination of rate of heat penetration in raw and coagulated tissue. Preparation of samples for subjective testing Cooked roasts were removed from the refrigerator after storage 0 . . . . for 15 hours at 4 C. Usmg the Hobart electric slicer, 16 slices were removed from the anterior end of each sample as shown in Figure 7. The first 2 slices were reserved for press fluid determinations; .mumoon poxooo mo msofimgmcwo o>floo33m pom o>floo30 ROM moHQEMm mo sofimooA .N. oudmfim Bdrm m monnm 41 uofluoumom sofimodmkm uofiuousaw o>floo35w 42 the remaining 14 slices were used as samples for the seven taste panel members. Two slices from a conventionally roasted sample and two slices from a radial-conventionally roasted sample were arranged on coded plates and covered with Saran. Preliminary taste panel Taste panel members were presented slices of beef from both conventionally and radial-conventionally cooked samples. The evalu- ators were asked to consider each roast without reference to the other. Factors to be scored were aroma, color, texture, flavor, juiciness, softness, friability, residual connective tissue, and tender- ness. A copy of the score card is included in the Appendix, page 87. Judges were asked to cut a 0. 50 to 0.75-inch square piece from the center of one slice from each roast sample and to count the number of chews required for complete mastication. They recorded the number of chews for each sample and also assigned a numerical tenderness score. From these evaluations, an individual "chew range" table was developed for and used by each judge for scoring samples for tenderness throughout the study. Preparation of samples for objective testing Raw samples for pH determinations were obtained from a 0. 5- inch slice removed from the posterior end of each roast. The location from which cooked samples were taken is shown in Figure 7. Procedures for obtaining objective test data were developed as follow 3 . 21:1. Readings of pH were recorded for slurries of both raw and cooked samples. Twenty grams of sample were chopped finely, placed in a Hotpoint electric blender with 100 ml. of distilled water 43 of known pH, and blended for three minutes. The resultant mixture was strained through a fine sieve into two 20 ml. beakers to permit duplicate readings for each sample. Press fluid. The first two slices removed from the anterior end of the cooked sample (Figure 7) were wrapped immediately in Saran, coded, frozen, and stored at -200C until completion of the experiment. Samples were removed from the freezer and defrosted at room temperature (23 to 250C) before press fluid determinations were made. Shear. After the 16 slices for taste panel evaluation and press fluid determination were removed, a distance of 3-inches was measured and the sample was cut vertically straight with a sharp knife. A core 1. 0-inch in diameter, cut parallel to the fibers, was removed from the center of each muscle. Each core was wrapped in Saran, coded, frozen, and stored at ~200C until completion of the experiment. Prior to shear testing, the cores were removed from the freezer and defrosted at room temperature (23 to 250C). Preroasting Preparation A schedule was formulated whereby samples were cooked twice weekly for a period of 3 weeks. On each cooking day, one roast was cooked by the conventional method and one roast was cooked by the radial-conventional method. Methods used for the cooking of samples were assigned according to a randomized arrangement of right and left muscles from the six pairs of rounds. Preparation of raw roasts Wrapped samples were removed from the freezer, placed on an . . . . 0 aluminum tray, and defrosted in a reach-1n refrigerator at 4 C for a 44 period of 48 hours. On the morning they were cooked, defrosted raw roasts were removed from the refrigerator, unwrapped, and a posterior portion removed for objective determination of pH. The ends of the roasts were cut vertically straight and the net weight and linear measurements of the trimmed sample were recorded. Volume was determined by the displacement method in 8 to 100C water. Although samples cooked conventionally were not split hori— zontally, each was tied firmly in five places with heavy cotton cord. The gross weight of the roasting pan and racks was recorded, the sample placed on the racks in the roasting pan, and the combined weight of the pan, racks, and sample recorded- Three potentiometer leads for recording data pertinent to time-temperature relationships during cooking and cooling were positioned in the sample at depths of 0.25-inch and 0.75-inch from the sample surface, and at the sample radius. Each of the samples cooked radial-conventionally was split lengthwise, the thermal rod inserted horizontally, and the sample tied with heavy cotton cord (Figures 2, 3 and 4). The gross weight of the roasting pan and racks was recorded, the sample positioned on the racks in the roasting pan and the combined weight of the pan, racks, and sample recorded. Three potentiometer leads were placed at depths of 0. 25-inch, 0.75—inch, and 1.0 inch from the sample surface (Figure 5). Cooking temperature For both the conventional and radial-conventional methods, the deck oven was preheated to 1490C, and the thermostat adjusted throughout the cooking period to maintain a controlled air temperature of 149°C. In addition, a constant setting of 20 per cent was main- tained on the powerstat for the radial—conventional method. 45 Cooking Pr oc e s 5 Conventional method When the internal temperature of the raw defrosted sample reached 10°C the sample and assembled apparatus were placed in the center of the preheated oven, and the oven door closed tightly. Each sample was cooked to an internal temperature of 80°C, as determined by the thermocouple positioned at the radius, then re- moved from the oven and cooled in the roasting pan at room tempera- ture to 700C. Then the thermocouples were removed, the strings cut, and the roast allowed to stand for 15 additional minutes. Radial-conventional method When the internal temperature of the raw defrosted sample reached 10°C the sample and assembled apparatus were placed in the preheated oven as shown in Figure 6. The thermal rod was connected to the electrical circuit, the oven door closed tightly, and the powerstat set at 20 per cent. When all potentiometer leads positioned in the sample reached a minimum internal temperature of 80°C, the thermal rod was dis- connected from the electrical circuit, the roast removed from the oven and cooled in the roasting pan at room temperature to 700C. Then the thermocouples were removed, the strings cut, the thermal rod removed, and the roast allowed to stand for 15 additional minutes. Treatment After Roasting Data from the cooked samples were collected both before chilling on the day of cooking and on the day after chilling. 46 Treatment of roasts before chilling Following the specified cooling period, the combined weight of the pan, rack, and drip; and the net weight of the sample were re- corded. Linear measurements and volume by displacement in 45 to 50°C water were recorded. The roast was then wrapped in Saran, coded, the anterior and posterior ends marked for identification, and refrigerated overnight at 40C. The weight in grams of the drippings was recorded. This weight was obtained by subtracting the known weight of the pan and racks from the total weight of the pan, racks and drippings. Treatment of roasts after chilling The day after the roasts were cooked, samples for objective and subjective evaluation were removed according to procedures described under Preliminary Investigations. Evaluation of Samples Nine palatability characteristics of the samples were evaluated subjectively by a seven member taste panel. Measurements of pH, press fluid, and tenderness were determined objectively. Panel scores, objective measurements, and cooking loss data were analyzed statistically. Data pertaining to rate of heat penetration for each method of cooking were studied. Subjective testing At consecutive testings each judge was presented with two slices from the same relative position in each roast. Samples were scored for aroma, color, texture, flavor, juiciness, softness, friability, residual connective tissue, and tenderness. Scores were based on a 47 seven point numerical scale. A score of 7 indicated excellent quality; a score of 1 represented unacceptable quality. The judges con- sidered descriptive terminology on the score sheet when assigning a numerical score for each palatability factor. Additional written comments by the judges were tabulated and included in the interpre- tation of the data. Objective te sting The pH of raw samples was taken the day of cooking; the pH of cooked samples the day after cooking. Objective measurements of press fluid and tenderness were made after completion of the cooking schedule . p_I-_i_. Slurries for pH determinations were prepared as described in the Preliminary Investigations. Duplicate readings, taken on the Beckman zeromatic pH meter, were averaged. Change in pH between the raw and cooked sample was calculated for each replication. Press fluid. Two samples, each weighing 12 to 14 grams, were cut from the cooked, defrosted slices from each roast, individually weighed and placed between felt pads. Each sample was subjected to a pressure of 15,000 pounds per square‘inch for 10 minutes in a Carver laboratory press. After pressing, samples were separated from the pads and reweighed. Per cent press fluid was calculated by dividing the difference between the initial and pressed weights by the initial weight of the sample and multiplying the resulting value by 100. Per cent press fluid yields for each replication and averages for each cooking method were determined. - Shear. Objective measurements of tenderness were determined by the Warner-Bratzler shear apparatus which measures pounds of force required to cut through a cylinder of muscle of definite diameter. 48 Four shear readings were taken from each defrosted 1.0 inch diameter core. These readings were totaled and averaged for each roast and grand averages for each cooking method were calculated. Cooking losses. Volume loss was calculated and converted to percentage of initial sample volume. Total cooking, drip, and volatile losses were determined for each roast and these values changed to percentages based on the raw weight of the sample. Changes in linear measurements effected by cooking were calculated and converted to percentages of initial sample measurements. Mean per cent linear changes were determined for each cooking method. Heat penetration During the cooking and cooling periods, progressive time- temperature relationships at three sample depths were recorded. For the radial-conventional method, these depths were 1.0 inch, 0.75-inch, and 0. 25-inch respectively. For the conventional method these depths were the radius, 0. 75—inch, and 0. 25-inch respectively. Rates of heat penetration, expressed as minutes per 0C rise, were calculated from the mean progressive time-temperature relationships at designated intervals for internal temperature for each depth for each method of roasting. Analysis of the data Analysis of variance was the statistical measure used to evalu- ate objective data pertaining to total cooking loss, drip loss, volatile loss, volume loss, shear, press fluid, and change in pH to determine differences attributable to method of cooking. For the subjective data, panel scores for each replication were averaged for each palatability factor to minimize variance due to 49 judges. Analysis of variance, based on the mean taste panel scores, was computed for each palatability factor to determine differences due to method of cooking. Correlation coefficients were determined for all possible com- binations of softness, friability, residual connective tissue, tender- ness, and shear measurement. In addition, per cent press fluid yield and subjective juiciness were correlated. RESULTS AND DISCUSSION The primary objective of this study was to compare the effects of conventional oven— roasting and a combination of internal radial heating and conventional oven- roasting on the palatability and yield of semitendinosus muscles from Choice grade steer beef rounds. Samples were tested both subjectively and objectively and the resultant data were analyzed statistically to determine differences attributable to cooking method. I A secondary objective was to examine the heat penetration data and investigate the potential for using such data as a valid basis for predicting roasting time for semitendinosus muscle of beef round. Mean progressive time-temperature relationships were collated to determine differences in rate of heat penetration due to cooking method. Palatability Subjective evaluations of aroma, flavor, color, texture, juici— ness, softness, friability, residual connective tissue, and tenderness were made by a panel of seven judges. The scoring scale range was from 7 to 1, indicating excellent to unacceptable quality, respectively. Judges considered descriptive terminology on the scoresheet when assigning a numerical score for each palatability factor. Average palatability scores for each replication appear in Table 13, the Appendix. Judges' numerical scores for each palatability factor were totaled and averaged for each roast and grand averages for method of cooking were computed. Grand averages, based on six replications for each method of cooking, for each characteristic evaluated are summarized in Table 1. 50 51 Table 1. Grand average palatability scores1 of six samples for two methods of cooking. Palatability Method of Cooking Characteristic Conventional Radial-Conventional Aroma 5. 1 4. 8 Flavor 5 . 0 4 . 9 Color 4. 3 3. 6 Texture 4.6 4. 7 Juiciness 4. 2 4. 7 Softness 4.4 4.4 Friability 3 . 0 2. 9 Residual Tissue 5.0 4.6 Tenderness 4.6 4, 2 lHighest possible score, 7 points. Aroma and flavor Grand mean palatability scores for aroma were 5. 1 and 4.8 for the conventional and radial-conventional methods, respectively. Descriptive terminology checked by judges ranged from a fair, faint aroma to a rich and meaty aroma. Analysis of variance computed on the mean aroma scores revealed no significant differences attribu- table to method of cooking or to animal. The aroma of the drip was noted by the investigator, For all replications, the aroma was full, rich, and meaty. Very little difference in flavor was detected by the judges. The grand average flavor score for the conventional samples was 5.0 as compared to 4.9 for the radial-conventional samples. A score of 5 indicated good, full flavor. No significant differences in flavor were found by analysis of variance of mean scores. The flavor of the drippings was also noted by the investigator. Drippings from the radial-conventional roasts were always full, rich and meaty with 52 a strong but acceptable salty taste. Although drippings from the conventional roasts had a full meaty flavor, a slightly charred taste was usually present. The analyses of variance of aroma and flavor scores are given in Table 2. Table 2. Analyses of variance of aroma and flavor scores for two methods of cooking. m m Source of M.S. Values Variance D. F. Aroma Flavor TOTAL 11 . ANIMAL 5 0. 02 0. 05 METHOD 1 0. 22 0. 01 ERROR 5 0.10 0. 06 Color and texture The external appearance of whole roasts varied slightly between the two methods of cooking. The exteriors of the conventional samples were slightly darker than the radial-conventional roasts and sometimes appeared almost charred in a few places. In judging samples for color, panel members noted that the color of radial-conventional roasts was not typical of well-done meat. The pink areas tended to be toward the outer edge of the sample rather than the center, but location was not consistent among repli- cations. Mean panel scores for color varied from 2. 9 to 4. 2, indi— cating a range of poor to desirable color for the radial-conventional samples. The grand mean score for color was 3.6 which indicated a mean evaluation of fair to good. 53 Grand average color score for the conventional method was 4.6 showing that judges tended to rate these samples higher than the radial-conventional samples. Mean color scores for individual roasts ranged from 3.6 to 4.7, fair to desirable. In three of the conventional samples, a few judges noted that the color was slightly pink and not typical of well-done meat. Analysis of variance of the mean color scores revealed no significant differences in color attributable to animal or to method of cooking. A slight iridescence was observed on the surface of samples from both methods of cooking. According to Weir (84), this is caused by the refraction of light in the fat layer spread by slicing. Both Masuda (54) and Lukianchuk (51) reported similar observations. Color of the drippings was noted by the investigator. Drippings from the radial-conventional roasts were a rich, red-brown color. During the cooling period, an orange-brown liquid seeped from the roasts. Drippings from the conventional roasts were very dark brown. During cooling, little if any liquid seeped from the roasts. These differences may be due to the longer cooking period required for the conventional roasts during which the drippings would tend to become more concentrated through evaporation. Textures of the radial—conventional and conventional roasts were judged very similar. Grand mean texture scores were 4. 7 and 4.6 respectively. These scores denote judgments of fair to good. Several judges described the pink areas of the radial-conventional roasts as spongy. A few samples were reported to have considerable amounts of noticeable thick connective tissue. Considering that the semitendinosus muscle is a less tender beef cut this is not an unusual observation. 54 Analyses of variance of the average texture scores revealed no significant differences attributable to animal or to method of cooking. A summary of the analyses of variance of color and texture mean scores is given in Table 3. Table 3. Analyses of variance of color and texture scores for two methods of cooking, Source of M.S. Value Variance D. F. Color Texture TOTAL 11 ANIMAL 5 0.08 0. 05 METHOD 1 1,47 0. 01 ERROR 5 0. 38 0. 05 Juiciness Judges indicated only a slight preference for the juiciness of the radial—conventional samples as compared to the conventional samples. Grand mean scores were 4.7 and 4.2 respectively. Panel members described all samples as juicy or as neither juicy nor dry. Objective evaluation of juiciness revealed that radial-conventional samples were more juicy than conventional samples. Mean per cent press fluid yields were 40.46 and 37.23, respectively. , Per cent press fluid yields for each replication are given in Table 14, the Appendix. Mean per cent press fluid yields and grand average juiciness scores for both methods are given in Table 4. No significant differences due to animal or to method of cooking were found by analysis of variance of mean juiciness scores. Analysis of variance of press fluid yields revealed no significant differences 55 Table 4. Average press fluid yields and grand average juiciness scores. Cooking Method % Press Fluid Juiciness Score Conventional 37. 23 4. 2 Radial-Conventional 40. 46 4. 7 1Highest possible score, 7 points. attributable to animal, but press fluid was greater (5% level of prob- ability) in the radial-conventional samples than in the conventional samples. The analyses of variance of juiciness scores and press fluid yields for two methods of cooking are presented in Table 5. Table 5. Analyses of variance of press fluid yields and juiciness scores for two methods of cooking. Source of M. S . Values Variance D. F. % Press Fluid Juiciness Score TOTAL 11 ANIMAL 5 13.29 0. 29 METHOD 1 31.42* 0.80 ERROR 5 4.13 0. 24 3:: Significant at 5% level of probability. The positive correlation (r = . 559) found between mean juici- ness scores and press fluid yields approached the 5% level of probability (r = .576). 56 Tenderness Grand average scores for softness, friability, residual con- nective tissue, and tenderness, and average shear values for the two methods of cooking are given in Table 6. Shear values of cooked samples for each replication are shown in Table 14, the Appendix. Table 6. Grand average scores1 for softness, friability, residual tissue, and tenderness and mean shear values for two methods of cooking. m m m Cooking Soft- Residual Tender- Shear Method ness Friability Tissue ness (lb) Conventional 4.4 3.0 5.0 4.6 17.19 Radial-Conventiona14.4 2.9 4.6 4.2 19.35 1Highest possible score, 7 points. Grand mean scores for softness were identical for the two methods of cooking. Mean softness scores for individual samples ranged from 3. 9 to 4. 7. In most instances, judges marked the descriptive term "neither hard nor soft. " Analysis of variance re- vealed no significant difference in softness attributable to animal or to method of cooking. Subjective evaluation of friability revealed that conventional roasts were rated only slightly higher than radial-conventional roasts. Grand mean score for the radial-conventional samples was 2. 9 as compared to 3. 0 for the conventional samples. The descriptive term for a score of 3 was “slightly friable. " Differences in friability due to animal or to cooking method were not statistically significant. In judging residual connective tissue, mean sample scores ranged from 4. 0 to 5. 3. Grand mean scores were 4. 6 for the 57 radial-conventional roasts and 5. 0 for the conventional roasts. Analysis of variance showed that residual connective tissue was significantly less (1% level of probability) in the conventional roasts than in the radial-conventional roasts. The smaller amount of residual connective tissue in the conventionally cooked roasts was probably due to increased conversion of collagen to gelatin during the longer cooking time required for this method. Tenderness ratings were subjectively determined by the number of chews required to completely masticate a sample of definite size. Grand mean scores for tenderness were 4.6 for the conventional samples and 4. 2 for the radial-conventional samples. Conventional samples were most often described as tender, whereas radial-con- ventional samples were most often scored as slightly tough. Analysis of variance of mean tenderness scores revealed a significant differ- once at the 5% probability level attributable to animal, and at the 1% level of probability attributable to method of cooking. Objective determinations of tenderness showed that conventional roasts were more tender than radial-conventional roasts. Mean shear values were 17.19 and 19. 35 respectively. However, analysis of variance for average shear readings showed no significant differ- ences attributable to animal or to method of cooking. The analyses of variance for softness, friability, residual connective tissue, tenderness and shear force are summarized in Table 7. The relationships between mean subjective scores for softness, friability, residual connective tissue, and tenderness and objective shear force measurements were studied. The correlation coefficients determined for all possible combinations of these factors are surn- marized in Table 8. 58 Table 7. Analyses of variance for softness, friability, residual connective tissue, and tenderness scores, and shear force values for two methods of cooking. M.S. Value Source of Soft- Residual Tender— Shear Variance D. F. ness Friability Tissue ness Force TOTAL 11 . . . . ANIMAL 5 0.08 0.13 0.15 0.17* 11,49 METHOD 1 0.00 0.05 0.52** 0.52M< 13.82 ERROR 5 0.07 0.04 0.03 0.02 5.40 * Significant at 5% level of probability. M: Significant at 1% level of probability. Table 8. Correlation coefficients for all possible combinations of softness, friability, residual tissue, and tenderness scores and Warner Bratzler shear measurements. Correlation Relationship Coefficient Softness/Friability . 182 Softness/Residual Tissue . 153 Softness/Tenderness . 148 Softness/Warner Bratzler Shear -. 091 Friability/Residual Tissue -. 007 Friability/Tenderness . 790** Friability/W arner Bratzler Shear -. 399 Residual Tissue/Tenderness . 855*96 Residual Tissue/Warner Bratzler Shear —.441 Tenderness/Warner Bratzler Shear -.656* * **Significant at the 5% level of probability. Significant at the 1% level of probability. 59 Significant positive correlations (1% probability level) were found between subjective evaluations of friability and tenderness, and between subjective evaluations of residual connective tissue and tenderness. Since it is known that the conversion of collagen to gelatin during cooking decreases the amount of connective tissue in cooked beef muscle and increases the ease with which muscle fibers can be separated, it seems reasonable to expect these relationships might be true. However, since the correlation coefficients obtained between subjective evaluations of friability and objective shear measurements and between subjective evaluations of residual con- nective tissue and objective shear measurements were not significant, the extent to which friability and residual connective tissue influence tenderness remains in doubt. A negative correlation, significant at the 5% level of prob- ability, was found between subjective tenderness scores and objective shear measurements. Correlation coefficients for all remaining relationships studied were not significant. Cooking Losses Percentages based on raw weight of sample, were calculated for total, drip, and volatile losses. Volume loss was converted to percentage of initial sample volume. Per cent total, drip, volatile, and volume losses for six replications for each cooking) method are recorded in Tables 15 and 16, the Appendix. Mean per cent values for cooking and volume losses for each cooking method are recorded in Table 9. Analyses of variance were computed for total, drip, volatile and volume losses to determine differences due to method and animal. Results of these analyses are summarized in Table 10. 60 Table 9. Mean per cent cooking and volume losses of six replications for two methods of cookery. Method of Cooking Losses Conventional Radial-Conventional % % Total cooking 30.111 26. 32 Drip 2. 14 3. 37 Volatile 27. 97 22. 94 Volume 28.51 30.25 lAverages based on six replications. Table 10. Analyses of variance of cooking and volume losses for two methods of cookery. Source of M.S. Values Variance D. F. Total Drip Volatile Volume TOTAL 11 . ANIMAL 5 6.64 0.21 8.8 31.83 METHOD 1 43.13=¢< 4.54** 75.76** 9.05 ERROR 5 3.08 0.19 3.11 32.14 >:< Significant at the 5% level of probability. >:<>:< Significant at the 1% level of probability. 61 Total cooking losses The average total cooking loss was 30. 11 per cent for con- ventionally roasted samples and 26. 32 per cent for radial-conven- tionally roasted samples. The conventionally cooked roasts had significantly greater total cooking losses then the radial-conventionally cooked roasts (5% level of probability). The average total cooking time was 64. 5 minutes for the radial-conventional samples compared with 125. 6 minutes for the conventional samples. Since, on the average, conventionally roasted samples required 48.6 per cent more cooking time than radial-conventional samples, it is probable that cooking time as well as method influenced total losses and also the ratio of drip to volatile losses. Drip losses The average per cent drip loss was 2. 14 for conventionally roasted samples and 3. 37 for the samples cooked by the radial- conventional method. Analysis of variance showed no significant differences attributable to animal, but drip losses were significantly greater (1% level of probability) for the radial-conventional samples than for the conventional samples. The shorter cooking period and the additional surface area which resulted from cutting the sample to insert the thermal rod could account in part, for the greater drip loss in the radial-conventional samples. Volatile los ses Mean volatile losses for conventional roasts and for radial- conventional roasts were 27.97 and 22. 94 per cent respectively. Analysis of the data showed the higher amount of volatile loss from the conventional roasts was significant at the 1% level of probability. 62 The greater part of volatile loss is from evaporation of water. Inasmuch as volatile loss was calculated on the basis of total weight loss minus weight of drippings, the longer cooking time required for the conventional method may have influenced evaporation of drippings as well as sample moisture. Volume losses Average volume losses for the conventional roasts and for the radial-conventional roasts were 28. 51 and 30.25 per cent, respectively. Analysis of variance for per cent volume loss indicated no significant differences among samples due to animal or method of cooking. Mean percentages for volume loss, changes in linear measure- ments and total weight loss for the two methods of cookery are shown in Table 11. Table 11. Mean percentages for volume loss, changes in linear measurements, and total weight loss for two methods of cookery. Method Conventional Radial-Conventional % % Volume loss 28. 511 30.25 Shrink in length 9. 61 9. 05 Shrink in width 2. 35 2. 35 Gain in depth 21.04 18.31 Total weight loss 30.11 26. 32 1 . . . Averages based on 81X replications Lowe (48) states that during cooking the shrink in volume in muscles of beef round is never as great as the decrease in weight. 63 Data in Table 11 indicate this to be true for conventional roasts, but not for roasts cooked by the radial-conventional method. Lukianchuk (51) had similar results. Her conventionally cooked samples had greater mean total weight loss than mean volume loss, but samples cooked in deep fat did not. According to Lowe (48), during cooking meat shrinks along the length of the fibers and in width but may gain in the third dimension until cooked extremely well done. The data from both methods used in this experiment support this premise. pH Determination of pH for raw and cooked samples, based on the average of two readings, and the change in pH for six replications for each cooking method are shown in Table 17, the Appendix. The pH of raw samples used for both cooking methods ranged from 5. 5 to 5.8. Winkler (87) studied the relationship between pH and tenderness in meat and reported toughness in raw meat to be maximum at pH 5.0 to 6. 0. Since the semitendinosus muscle is usually classified as a less tender portion of the beef carcass, the pH values of the raw test roasts in this study were in accord with Winkler's findings. In all cases cooked roasts were slightly more alkaline than raw roasts. The pH of samples roasted by either method varied from 5. 8 to 6. 0. Changes in pH brought about by cooking ranged from 0. 2 to 0. 3 for the conventionally roasted samples and from 0. 2 to 0.4 for the radial-conventionally roasted samples . 64 Heat Penetration Due to difficulty in obtaining accurate potentiometer recordings during one day of cooking, results and discussion of data pertaining to rate of cooking are based on five replications for each method. Data pertaining to time-temperature relationships during cook- ing were obtained from potentiometer leads positioned at three depths from the surface of the sample: 0.25-inch, 0.75-inch, and sample radius (approximately 1. 5 inches) for the conventional method and 0. 25-inch, 0.75-inch, and l. 0 inch for the radial-conventional method. The lead positioned with depth equal to the sample radius was used to determine the end of the cooking period for the conventional method. For the radial-conventional method all leads were required to reach a minimum of 80°C before samples were removed from the oven. Average initial sample temperatures for depths of 0. 25-inch, 0.75-inch, and the radius were 13.4, 10.0, and 9.40C, respectively. Mean progressive time-temperature relationships during cooking for three sample depths and two methods are shown in Figure 8. All curves for roasts cooked by the radial-conventional method were somewhat steeper and considerably shorter than the comparable curves for conventionally roasted samples. The internal temperature of samples cooked by the radial-conventional method increased steadily and rapidly throughout cooking. Although the rise in temperature for samples cooked by the conventional method was much slower, the rate of rise was steady until an internal temperature of 50°C was reached; thereafter the rate of rise decreased continuously. Roasts cooked conventionally required nearly twice as much time to reach an end cooking temperature of 80°C as those cooked by the radial-conventional method. Average cooking time was 126. 5 minutes for conventionally roasted samples compared with 64. 5 minutes for the 65 . mpofioe manoo OB» HOH mam—mop 035mm mount. Mom mcgooo mcwudp mafiamcoflmfiou ongouomEounoEfi ommuo>< .m oudmfih \ \Q as \. $15.1“ ifixx Am. 49% \o\ o\ “fififilxlfififii . C. o . \.\ o 1333.3! _ .\. $6» Hmcoflc o>coU Hocofldoraou \ _ xJnXXXXXXX _III -soeoom cal o: 03 cm 00 ca» ON mofiESz Iii u u 1+. u L n v o Smcofidocaoouagpmt £03 o4 .. Smcoficmtaoov £03 m4 . sodwumwd .. -I...Iu.l. cocfiummd .. 500060» ">0! .\ o\ .\ \u.\ ...ow 66 radial—conventional samples, a difference of 61. 1 minutes for roasts of comparable size, shape, and composition. It seems reasonable to expect that by adding a second heat source, the thermal rod, cooking time would be decreased. Furthermore, by locating the rod horizontally at the radius of the roast and simultaneously apply- ing heat to the interior as well as the exterior of the roast, the depth of tissue which the heat from each source must penetrate before they meet is reduced. As was expected, the mean end cooking temperatures of con- ventional roasts at depth locations 0. 25-inch and 0. 75-inch from the sample surface exceeded the internal temperature at the radius; 91.4°c for the o. 25-inch depth and 86.00C for the o. 75-inch depth. In the samples roasted by the radial-conventional method, however, mean end temperatures for the 0. 25-inch and 0. 75—inch depth loca- tions were 79. 3 and 81. 80C, respectively. Variations in end cooking temperatures at similar depths among replications were apparent in both methods. Inherent differences in the samples, changes in linear measurements brought about by evaporation and coagulation of the tissue, and experimental error in the placement of the potentiometer leads appeared to contribute appreciably to variations among replications within method. At the end of the cooking period roasts were removed from the oven and allowed to stand at room temperature until the temperature of the lead which had determined the end of the cooking period had cooled to 700C. The internal temperature rise of roasts after removal from the oven was similar for both methods, varying only from 0 to 20C among replications within each method. Progressive changes in the mean rate of heat penetration during cooking, expressed as minutes per degree Centigrade rise at 10- degree intervals from 20 to 800C, for two cooking methods at three 67 sample depths are shown in Figures 9 and 10. Changes in rate of heat penetration for each replication for each method are given in Tables 18 and 19, the Appendix. After an internal temperature of 350C was reached, the mean heat penetration curves for radial-conventional roasts at sample depths of 0. 75-inch and 1.0 inch were similar in shape and showed a steady decline in rate of penetration throughout the remainder of the cooking period. Although the rate of heat penetration was initially high at the 0. 25-inch sample depth, there was a steady moderate decrease until the temperature of the sample at this depth reached 600C. From 60 to 70°C the rate decreased sharply but after the sample reached 700C there was a slight increase in rate of heat penetration (see Figure 9). Variations in mean heat penetration rates for all sample depths studied were more pronounced in conventionally roasted samples than in radial-conventional samples. Rate of heat penetration was steady at the 0. 75-inch depth to a temperature of 500C. Thereafter there was a sharp decrease throughout the remainder of the cooking period. Curves for the 0. 25-inch depth and radius depth were similar in out- line. There was a rapid rise in rate to a temperature of 40°C and then a steady decline thereafter. The 0.25-inch depth showed a sharp decrease in rate between temperatures of 40 and 500C and a very sharp decline between temperatures of 70 and 800C. As was anticipated because of their proximity to the source of heat, the initial rise in rate of heat penetration was highest at the 0. 25-inch depth and lowest at the radius depth. Throughout most of the cooking period the rate was generally highest at the 0. 25-inch depth and lowest at the radius depth. The rates of heat penetration at internal temperature inter- vals between 60 and 700C and 70 and 80°C were similar at all sample depths . 68 Min/0C rise 60 70 80 ALAAAL‘ 1.25 1.50- 1075‘I 2. 00... 2.25. 2.50-- 2.75< 3.00. V Protein Coagulation Key: WW - 0.25 inch ..-—.-. " 0.75 inch " 1.0 inch Rare Medium Well-done ; mAAA l l L ‘ v vAvvvva T 30 40 50 60 70 80 Figure 9. Average rate of heat penetration: Min/0C rise for three sample depths for the radial-conventional cooking method. o 69 Min/ C rise 0 20 30 40 50 60 70 80 ‘AA“’ a r I vvvvi i \AAAAAAA fTWVV v v— Protein Coagulation .25“ Rare .50 '- + 1.00? I 1.25 7 Well-done 1.50 - I l.75-- 2.00 ., 2.25 ~— 2.50. T 2.75 .. Key: 3,00 ,_ ' xmxxx - 0.25-inch --—-- - 0.75-inch a 3'25 .. --—--- - 1.5 inch . 3050 7" MALI 1 A A Iv'v' r I I C 0 20 30 40 50 60 70 80 Figure 10. Average rate of heat penetration: Min/0C rise for three sample depths for the conventional cooking method. 70 Lowe (48) states that according to Sprague and Grindley internal temperatures ranging from 55 to 65°C represent rare beef; those ranging from 70 to 800C denote well-done beef. For comparison of rate of heat penetration for the two cooking methods used in this study, temperatures of 50, 60, 70, and 80°C were chosen as indicative of the following degrees of doneness: beginning of muscle coagulation, rare, medium, and well-done. The comparative ranges in minutes per degree Centigrade rise between stages of doneness for the three sample depths for the two cooking methods are illustrated in Figures 9 and 10. Except between temperatures of 70 and 80°C at the 0. 25-inch location in the radial-conventional samples, at all depths measured there was a decrease in rate of heat penetration as the state of muscle coagulation was approached and a continuous decline throughout the remainder of the cooking period. This phenomenon is attributed to the fact that evaporation and muscle coagulation which occur during cooking are endothermic processes. Consequently, less heat is available for elevation of muscle temperature. For each sample depth measured, the rate of rise in temperature for radial-conventional roasts exceeded that of the conventional roasts. These results were anticipated due to the additional heat source in the radial-conventional method. At the exterior surface of each sample there is a layer of immobile air which acts as an insulator between the heat source and the sample. It is believed that when the surrounding oven air is put in motion by the heat energy applied, the insulating air layer gradually becomes thinner and the temperature of the meat at the surface increases. Between the thermal rod and the sample surface there is a very much thinner insulating air layer. As a result, heat from the rod is more easily transferred to the meat. In addition, without considering the effect of the insulating air layer, 71 metal is a better conductor of heat than air and thus heat transfer could be more efficient. Considering both of these factors, at any given time there should be a greater amount of heat within the radial- conventional samples and, consequently, the rate of heat penetration within the samples would exceed that of the conventional samples. SUMMARY AND CONCLUSIONS The objectives of this study were to compare the effects of conventional oven- roasting and a combination of internal radial heat- ing and conventional roasting on the palatability and yield of semi- tendinosusmuscles from paired U. S. Choice steer beef rounds, and to investigate the potential for using heat penetration data as a valid basis for predicting cooking time for semitendinosus muscle of beef round. Procedures used for the preparation, roasting, and sub- jective and objective evaluations of the samples were developed through preliminary investigations in the laboratory. Semitendinosus muscles were dissected, cleared of surface fat, and trimmed as comparable as possible in shape within a weight range of 1300 to 1500 grams. Individual raw roasts were wrapped, frozen, and stored at ~20°C until defrosted for testing. Prior to cooking, each sample was defrosted and weighed. . ,Data pertaining to linear and volumetric measurements were recorded. Roasts cooked by the radial-conventional method were split length- wise to a depth equal to the sample radius, the thermal rod inserted horizontally, and the roast tied securely with heavy cotton cord. Roasts cooked conventionally were tied in'a like manner. Potentiometer leads were positioned at three depths as measured from the surface of the roast: 0.25-inch, 0.75-inch, and sample radius for the conven- tional method, and 0. 25-inch, 0. 75-inch, and 1. 0 inch for the radial- conventional method. Six replications, for each cooking method were performed. For both methods, the deck oven was preheated to 1490C, and this air temperature maintained throughout the cooking periods. 72 73 In the radial-conventional method a powerstat, set at 20 per cent, was used to reduce the current entering the thermal rod to 22 volts. All roasts were cooked to an internal temperature of 80°C. In the conventional samples internal end temperature was determined by the thermocouple positioned at the radius; in the radial-conventional samples all potentiometer leads in the roasts were required to reach a minimum temperature of 80°C before the roast was removed from the oven. Samples roasted by either method were cooled at room temperature to 70°C before the leads (conventional samples) and the leads and thermal rod (radial-conventional samples) were removed. All samples were allowed to stand 15 additional minutes before objective data for cooked roasts were recorded. A seven member taste panel scored all samples for aroma, flavor , color, texture, juiciness, softness, friability, residual connective tissue, and tenderness. Measurements of pH, press fluid and shear were determined objectively. Total cooking, drip, volatile, and volume losses were recorded for each roast. Data pertaining to rate of heat penetration were studied to evaluate differences attribut- able to cooking method. Grand average palatability scores for conventionally roasted samples were higher for aroma, flavor, color, friability, residual tissue and tenderness than for comparable grand averages for radial- conventional samples. A very slight preference was recorded for the texture of the radial-conventional samples although several judges described areas of some samples as spongy. Grand mean softness scores were identical for the two cooking methods. Statistical analyses revealed no significant differences in aroma, flavor, color, friability, texture or softness attributable to method of cooking. 74 Judges favored the juiciness of the radial-conventional samples. Samples were described as juicy or as neither juicy nor dry. Mean per cent press fluid yield was higher for the radial-conventional samples than for the conventional samples. Analysis of variance of mean juiciness scores revealed no significant differences attributable to animal or method of cooking. Statistical analysis of per cent press fluid values however revealed significant difference due to method of cooking. A positive correlation coefficient determined for juiciness/ per cent press fluid approached the 5% level of significance. Grand mean scores for tenderness favored the conventionally roasted samples. Conventional samples were most often scored as tender, whereas radial-conventional samples were most often scored as slightly tough. Analysis of variance of mean tenderness scores revealed highly significant differences in tenderness attributable to method of cooking. Analysis of variance also showed that residual connective tissue was significantly less (1% level of probability) in conventional samples. All cooked samples had slightly higher pH values than raw samples. However, changes in pH brought about by cooking were not significant for method. Conventionally cooked roasts had significantly greater total cooking losses than samples cooked by the radial-conventional method. Analysis of variance of the data showed drip losses in the radial- conventional samples were significantly greater (1% level of probability) than drip losses in the conventional samples, whereas volatile losses were significantly greater (1% level of probability) in conventional roasts than in radial-conventional roasts. Mean volume loss was higher for radial-conventional samples than for conventionally roasted samples. Conventional samples had higher total weight losses than radial-conventional samples. 75 Roasts cooked by the radial-conventional method revealed greater volume loss than weight loss whereas for the conventional roasts this relationship was reversed. Positive correlation coefficients, significant at the 1% prob- ability level, were found between friability/tenderness and residual connective tissue/tenderness. A negative correlation coefficient, significant at the 5% level of probability, was found between Warner Bratzler shear/tenderness. In all replications, the conventional roasting required approxi- mately twice the cooking time required for radial-conventional roasting, and, irrespective of method, rate of temperature rise generally decreased as muscle coagulation progressed. Mean end cooking temperatures at the three lead depths showed a smaller range in the radial-conventional samples than in the con- ventional samples. The ranges of internal temperature rise during the cooling periods were comparable for both cooking methods. No appreciable difference in rates of heat penetration was noted at the three sample depths in the radial-conventional method whereas the rates of heat penetration at the three sample depths in the con- ventional method showed greater variance from each other. Rate of heat penetration at all depths in the radial-conventional samples was faster at any given time than in the conventional samples. Within the limits of this investigation the following conclusions are indicated: 1. With the exception of tenderness and residual connective tissue, semitendinosus samples cooked by a combination of radial heating and conventional roasting compared favorably with similar samples cooked conventionally for all palatability factors scored. 2. Per cent total cooking losses were decreased by use of the radial-conventional method. Although per cent drip losses were 76 higher in the radial-conventional method, per cent volatile losses were lower than in the conventional method. Because of the shorter cooking period and the split surface in the radial-conventional samples there was more opportunity for drippings to form and remain. With the longer cooking period more evaporation could occur and conse- quently higher volatile losses'were evident in the conventional samples. Volume losses were higher in the radial-conventional samples. It is conceivable that the increased total heat within the radial-conventional samples could account, in part , for volume losses exceeding those of the conventional samples. 3. Total cooking time was reduced nearly 50 per cent by use of the radial-conventional method. Because of the increased heat avail- able from two sources, heat transfer to and through the muscle tissue was more rapid in this method. 4. The theory that rate of heat penetration decreases as muscle coagulation progresses is strongly supported by this study. In both cooking methods, minutes per degree Centigrade rise increased as samples approached the beginning of muscle coagulation and continued to increase as muscle coagulation progressed. The results of this study show that the radial-conventional method has potential as an acceptable means for cooking semitendinosus muscle of beef round. If a means could be devised to insert the thermal rod without splitting the roast, more moisture might be retained by the samples and sample tenderness might consequently be increased. Furthermore, if radial-conventional samples were cooked at a slightly lower oven temperature it is reasonable to expect both cooking time and cooking losses would increase slightly but it is also likely that tenderness would increase and residual connective tissue decrease. 10. LITERATURE CITED . Aldrich, P. J. Good and Choice beef rounds: effect of extent of cooking on palatability and edible portion. Unpublished Ph. D. Thesis. Arnes Iowa, Iowa State College Library. 1951. Aldrich, P. J. and Lowe, B. Comparison of grades of beef rounds. J. Am. Diet. Assn. 30:39-43. 1954. Alexander, L. M. Shrinkage of roast beef in relation to fat content and cooking temperature. J. Home Econ. 22:915-922. 1930. Alexander, L. M. and Clark, N. G. Shrinkage and cooking time of rib roasts and beef of different grades as influenced by style of cutting and method of roasting. U. 5. Dept. Agr. Tech. Bul. 676. 1939. . Alexander, L. M. and Clark, N. G. Shrinkage and heat penetration during roasting of lamb and mutton as influenced by carcass grade, ripening periods, and cooking methods. U. 5. Dept. Agr. Tech. Bull. 440. 1934. . Barbella, N. G., Hankins, O. G., and Alexander, L. M. The in- fluence of retarded growth in lambs on flavor and other character- istics of meat. Proc. Am. Soc. Animal Prod. 29:289-294. 1936. . Birkner, M. L. and Auerback, E. Microsc0pic structure of animal tissue. In The Science of Meat and Meat Products. American Meat Institute Foundation. W. H. Freeman and Company, San Francisco and London.. 1960. Boggs, M. M. , and Hanson, H. L. Analysis of foods by sensory difference tests. Advances in Food Res. 2:219-258. 1949. Brady, D. E. A Study of factors influencing tenderness and texture of beef. Proc. Am. Soc. Animal Prod. 30:246-250. 1937. Bramblett, V. D., Hostetler, R. L., Vail, G. E., and Draudt, H. N. Qualities of beef as affected by cooking at very low temperature for long periods of time. Food Tech. 13:707-711. 1959. 77 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 78 Branaman, G. A., Hankins, O. G., and Alexander, L. M. The relation of degree of finish in cattle to production and meat flavors. Proc. Am. Soc. Animal Prod. 29:295-300. 1936. Bratzler, L. J., Warner, K. F., and MacKintosh, D. L. Physical research on meat with special reference to tenderness. Cooperative Meat Investigations, Report of the Review Committee, Vol. 1, Ref. 18. 1937. Child, A. M. Selection and use of pork cuts. Minn. Agr. Expt. Sta. Bull. 254. 1929. Child, A. M., and Baldelli, M. Press fluid from heated beef muscle. J. Agr. Res. 48:1127. 1934. Child, A. M. and Esteros, G. A study of the juiciness and flavor of standing and rolled rib roasts. J. Home Econ. 29:182-187. 1937. Child, A. M. and Fogarty, J. A- Effect of interior temperature of beef muscle upon the press fluid and cooking losses. J. Agr. Res. 51:655-662. 1935. Child, A. M. and Satorius, M. J. Effect of exterior temperature upon the press fluid, shear force, and cooking losses of roasted beef and pork muscles. J. Agr. Res. 57:865-871. 1938. Clark, R. K. and Van Duyne, F. O. Cooking losses, tenderness, palatability, and thiamine and riboflavin content of beef as affected by roasting, pressure saucepan cooking, and broiling. Food Res. 14:221-230. 1949. Cline, J. A. and Foster, R. The effect of oven temperature on beef roasts. Mo. Agr. Expt. Sta. Bul. 328. 1933. Cline, J. A., Loughead, M. E., and Schwartz, B. C. The effect of two roasting temperatures on palatability and cooking losses of roasts. Mo. Agr. Expt. Sta. Bul. 310. 1932. Cline, J. A. Trowbridge, E. A., Foster, M. T., and Fry, H. E. How certain methods of cooking affect the quality and palatability of beef. Mo. Agr. Expt. Sta. Bul. 293. 1930. Cover, S. Comparative cooking time and tenderness of meat cooked in water and in an oven of the same temperature. 1 (Abstract.) J. Home Econ. 33:596. 1941. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 79 Cover, S. Effect of metal skewers on cooking time and tenderness of roasts. Texas Agr. Expt. Sta. Bul. 542. 1937. Cover, S. Scoring for three components of tenderness to character- ize differences among beef steaks. Food Res. 24:564-573. 1959. Cover, S. The effect of temperature and time of cooking on the tenderness of roasts. Texas Agr. Expt. Sta. Bul. 542. 1937. Crist, J. W. and Seaton, H. L. Reliability of organoleptic tests. Food Res. 6:529-536. 1941. Davis, J. G. and Hanson, H. L. Sensory test methods. I. The triangle intensity and related test systems for sensory analysis. Food Tech. 8:335-338. 1954. Day, J. C. Longissimus dorsi of three grades of beef; comparison of cooking weight losses, palatability, and edible portion. Unpub- lished M. S. Thesis. East Lansing, Michigan. Michigan State University Library. 1953. Doty, D. M. Methods of analysis. In Science of Meat and Meat Products. American Meat Institute Foundation. W. H. Freeman and Company. San Francisco and London, 1960. Doty, D. M. Thermal processing. In Science of Meat and Meat Products. American Meat Institute Foundation. W. H. Freeman and Co. San Francisco and London, 1960. Dove, W. F. Food acceptability: its determination and evaluation. Food Tech. 1: 39-50. 1947. Dunnigan. J. H. A study of palatability and price of two grades of sirloin butts. Unpublished M. S. Thesis. East Lansing, Michigan. Michigan State University Library, 1943. Foster, D., Pratt, G., and Schwaitz, N. Variation in flavor judg- ments in a group situation. Food Res. 20:539-544. 1955. Grindley, H. S., McCormack, H., and Porter, H. C. Experi- ments on losses in cooking meat. U. 5. Off. Expt. Sta. Bul. 102. 1901. Gridley, H. S. and Mojonnier, T. Experiments on losses in cook- ing meat, 1900 and 1903. U. S. Dept. Agr. Off. Expt. Sta. Bul. 141. 1904. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 80 Griswold, R. M. The effect of different methods of cooking beef round of Commercial and Prime grades. I. Palatability and shear values. Food Res. 20:160-170. 1955. Griswold, R. M. and Wharton, M. A. Effect of storage conditions on the palatability of beef. Food Res. 6:517-528. 1941. Halliday, E. G. Objective tests for cooked food. Food Res. 2:287-288. 1937. Hankins, O. G. and Ellis, N. R. Fat in relation to quantity and quality factors of meat animal carcasses. Proc. Am. Soc. Animal Prod. 32:315-319. 1939. Hanson, H. L., Stewart, G. F., and Lowe, B. Palatability and histol%gical shanges occurring in New York dressed broilers held at 1.7 C (35 F). Food Res. 7:148-160. 1942. Harrison, D. L. Histological, physical, and organoleptic changes in three grades of beef during aging. Unpublished Ph. D. Thesis. Ames, Iowa, Iowa State College Library. 1943. Harrison, D. L. Shrink, rate of heat transfer, and palatability of beef cooked at the same temperature in air, steam, water, and fat. Unpublished M. S. Thesis. Ames, Iowa. Iowa State College Library. 1943. Helser, M. D., Nelson, P. M., and Lowe, B. Influence of the animals' age upon the quality and palatability of beef. Iowa Agr. Expt. Sta. Bul. 272. 1930. Hiner, R. L., and Hankins, C. G. Temperature of freezing affects tenderness of beef. Food Ind. 19:1078-1081. 1947. Hurwicz, H. , and Tischer, R. G. Variation in determinations of shear force by means of Bratzler-Warner shear. Food Tech. 8: 391. 1954. Latzke, E., Cooking and canning meat. N. Dakota Agr. Expt. Sta. Circ. 137. 1934. Latzke, E. Standardizing methods of roasting beef in experimental cookery. North Dakota Agr. Expt. Sta. Bul. 242. 1930. 48. 49. 50. 51. 52. 53. 54. 55. 56. 57. 81 Lowe, B. Experimental cookery. 4th ed. New York, John Wiley and Sons Inc., pp. 192-251. 1955. Lowe, B., Crain, E., Amick, G., Riedesol, M., Peat, L. J., Smith, F. B., McClurg, B. R., and Shearer, P. S. Defrosting and cooking frozen meat. Iowa State Col. Agr. Expt. Sta. Res. Bul. 385. 1952. Lowe, B. , and Stewart, G. F. Subjective and objective tests as food research tools with special reference to poultry meat. Food Tech. 1:30-38. 1947. Lukianchuk, Z. J. Effect of two methods of dry heat cookery on palatability and cooking losses of semimembranosus muscle of beef round. Unpublished M. S. Thesis. East Lansing, Michigan. Michigan State University Library. 1960. MacKintosh, D. L., Hall, J. L., and Vail, G. Some observations pertaining to tenderness of meat. Proc. Am. Soc. of Animal Prod. 29:285-289. 1936. Marshall, N., Wood, L., and Patton, M. B. Cooking Choice grade, top round beef roasts. J. Am. Diet. Assn. 36:569-573. 1959. Masuda, G. M. Tender cuts of three grades of beef: effect of extent of cooking on weight losses and cost. Unpublished M. S. Thesis. East Lansing, Michigan. Michigan State University Library. 1955. McCance, R. A. and Shipp, H. L. The chemistry of flesh foods and their losses on cooking. Spec. Rpt. Series, No. 187. Medical Research Council. London, 1933. Mitchell, H. H., Hamilton, T. S., and Hanes, W. T. Some factors affecting the connective tissue content of beef muscle. J. Nutrition 1:165-178. 1928. Moran, T., and Smith, E. C. B. Postmortem changes in animal tissues: The conditioning or ripening of beef. Great Britain Dept. Scientific Ind, Res. Food Investigations Board. Spec. Rpt. No. 26. 1929. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 82 Morgan, A. F. and Nelson, P. M. A study of certain factors affecting the shrinkage and speed in the roasting of meat. J. Home Econ. 18:371-378, 444-448. 1926. National Cooperative Meat Investigations, Committee on Prepara- tion Factors. Meat and meat cookery. Chicago Nat'l Livestock and Meat Board. Pp. 129-166. 1942. Noble, 1. T., Halliday, E. G., and Klaas, H. K. Studies on tenderness and juiciness of cooked meat. J. Home Econ. 26: 238-242. 1934. Paul, P. C. Changes in palatability, microscopic appearance, and electrical resistance in beef during the onset and passing of rigor and during subsequent storage. Unpublished Ph. D. Thesis. Ames, Iowa. Iowa State College Library, 1943. Paul P. and Bratzler, L. J. Studies on tenderness of beef. III. Size of shear cores: end to end variation in the semimembranosus and adductor. Food Res. 20:635-638. 1955. Paul, P. and Child, A. M. Effect of freezing and thawing beef muscles upon press fluid, losses, and tenderness. Food Res. 2:339-347. 1937. Paul, P. C., Lowe, B., and McClurg, B. R. Changes in histo- logical structure and palatability of beef during storage. Food Res. 9:221-223. 1944. Pearson, A. M., and Miller, J. I. The influence of rate of freezing and length of freezer storage upon the quality of beef of known origin. J. Animal Science 9: 13. 1950. Peret, H. Taste panel tests. Natl. Provisioner. 121:12-13. 1949. Problems of taste testing. (News release.) J. Am. Diet. Assn. 30:756. 1954. Proctor, B. E., Davison, S., and Brody, A. L. A recording strain-gauge denture tenderometer for foods. II. Studies on masticatory force and motion and the force penetration relation- ship. Food Tech. 7:327. 1956. 69. 70. 71. 72. 73. 74. 75. 76. 77. 78. 79. 80. 83 Ramsbottom, J. M. Freezer storage effect on fresh meat quality. Refrig. Eng. 53:19-23. 1947. Ramsbottom, J. M., and Strandine, E. J. Comparative tender- ness and identification of muscles in wholesale beef cuts. Food Res: 13:315-330. 1948. Ramsbottom, J. M., Strandine, E. J., and Koonz, C. H. Compara- tive tenderness of representative beef muscles. Food Res. 10: 497-509. 1945. Satorius, M. J. and Child, A. M. Effect of coagulation on press fluid, shear force, muscle-cell diameter and composition of beef muscle. Food Res. 3: 619-626. 1938. Satorius, M. and Child, A. M. Effect of cut, grade, and class upon palatability and composition of beef roasts. Minn. Agr. Expt. Sta. Tech. Bul. 131. 1938. Satorius, M. J. and Child, A. M. Problems in meat research. 1. Four comparable cuts from one animal. II. Reliability of judges scores. Food Res. 3: 627-635. 1938. Schultz, H. W. Mechanical methods of measuring tenderness of meat. National Livestock and Meat Board, Proceedings of the 10th Annual Reciprocal Meat Conference, 17. 1957. Simon, M., Carroll, F., and Clegg, M. T. Effect of degree of finish on differences in quality factors of beef. Food Res. 23: 32-40. 1958. Stech. O. P. and West, G. M. Roasting meat at 2500F. J. Am. Diet. Assn. 30:160. 1954. Tanner, B., Clark, N. G., and Hankins, O. G. Mechanical determination of juiciness of meat. J. Agr. Res. 66:403. 1943. Thille, M., Williamson, L. J., and Morgan, A. F. The effect of fat on shrinkage and speed in roasting beef. J. Home Econ. 24:720-733. 1932. Towson, A. M. Palatability studies of beef rib roasts. I. As affected by high versus low oven temperatures. Unpublished M. S. Thesis. Ames, Iowa. Iowa State College Library. 1940. 84 81. Vail, G. E. and O'Neill, L. Certain factors which affect the 82. 83. 84. 85. 86. 87. palatability and cost of roast beef served in institutions. J. Am. Diet. Assn. 13:34-39. 1937. Visser, R. U., Harrison, D. L., Goertz, G. E., Bunyan, M., Skelton, M. M. , and MacKintosh, D. L. The effect of degree of doneness on the tenderness and juiciness of beef cooked in the oven and in deep fat. Food Tech. 14:193-198. 1960. Weir, C. E. Methods of analysis. In Science of Meat and Meat Products American Meat Institute Foundation. W. H. Freeman and Company. San Francisco and London. 1960. Weir, C. E. Palatability characteristics of meat. In the Science of Meat and Meat Products. American Meat Institute Foundation. W. H. Freeman and Company. San Francisco and London. 1960. Wierbicki, E., Kunkle, L. E., and Cahill, V. R., and Deatherage, F. E. The relation of tenderness to protein alterations during postmortem aging. Food Tech. 8:506-511. 1954. Winegarden, M. W., Lowe, B., Kastelic, J., Kline, E. A., Plagge, A. R., and Shearer, P. S. Physical changes of connective tissue of beef during heating. Food Res. 17:172. 1952. Winkler, C. A. Tenderness of meat. I. A recording apparatus for its estimation and relation between pH and tenderness. Can. J. Research, D 17, 8, 1939. APPENDIX 85 86 Table 12. Weight of rounds, muscle weight, maximum linear measure- ments of muscles, and trimmed sample weight. Weight Weight Maximum Linear Trimmed Left Rump On Muscle Measurements (in.) Sample Round (lb. ) (gm. ) Length Width Depth (gm. ) 1 79.6 1571 13.50 4.13 2.63 1247 2 84.5 1966 13.38 4.75 3.13 1256 3 76.0 1567 13.00 4.00 2.63 1174 4 70.2 1532 12.75 3.75 2.88 1149 5 72.4 1426 13.00 3.88 2.63 1131 6 79.0 1652 13.88 3.88 2.75 1118 Right Round 1 77.0 1535 13.63 3.88 2.63 1170 2 76.2 1875 13.25 4.63 3.00 1294 3 76.1 1560 12.25 4.13 2.75 1204 4 67.2 1499 12.50 4.13 2.75 1179 5 70.8 1425 12.38 3.88 2.63 1152 6 75.0 1653 14.13 4.00 2.75 1209 ULKU QQUQV. S13 2. 7 8 8500 38:0 p.80 ouoom 8.82 deoo. swag. nwdou Henson Hopcou cofimmmumflfi >HoEonuxM >uo> Awdofi >fiEmSm .8988 >.8> >888Huxm mmocnopcorfi ..H..U v.8: ..H.U pnmfi .H.OE.~$ ..H.O 53w ..H..U umOm ..H..U umOm flow oommfi. mo 85088 mo ngofim Mo «5858 Mo 85058 mo E5088 mo 8.9088 ..H .0 .02 8388580 emumd 5:882 8882 2885 Seam >238 Hmsgmou mo 888 one .894 8322: 8588“ >H 83.88% 8323 83233 83.3.3 82 13me >.8> >S£m3m >88uopoz van—88h >.8> >8883xm >u3388h >298 30m fi0m Bozo Cu v.8: >uo> them you been flow “Home >uo> >858be mmoaumom v.8: ooB uofifioz >meo . Nap >35.“ BOZmBm >up >.8> >HQ .Hoc >33. >336 >08“. >.8> >8883xm mmofiogh 8. >36 COM. .8582 unammmacD >888 .8 “:13 ..fimh 38h 88w .6000 25% 8000 >885 >.8> .fiofim A58: Hoe/3h msflxowd >wc8um wwcmnum omumoo poom HmHSmon .omnmoo >uo> .mmnmou >13me 0“ 38h U000 Edam warm 88¢ >Ho> 889889 ponodoomflfl boom 8.393 oBmEmop Room” thQ fish on 38h .pooD ozmfimofl >.8> .880 50.69883 ucmmmmacb >885 no uafimm £83m Adah 88m 6000 :3 .6000 >885 >um> .nofim 888.3% mafixomd H N m w. m o N. mama codewoflflcog @993. .02 838m 88 or. 0.... o..m «6 Ne or. me oh Tm oweeoae. cameo $.$ m.$ >.N 06 $.m >.$ $.$ m.$ 8m $.$ o.$ o.~ m.$ ofo. >.$ m.$ o.m m.m m.$ >.$ min. o.$ o.$ o.$ >.$ 2m m.m >.$ o.m ole. m.$ o.$ $.$ bio. 2m $.m w.$ m.m N.m >.$ m.$ m.$ N.$ m.$ w.$ o.m 1m $.m m.$ m.m m.$ $.$ o.m >.$ Hmcofls8>soo me or. min we e6 We 8m me we omoeofiw H.$ >.$ otm m.$ m.$ $.$ >.$ $.$ >.$ 98MB H.$ $.$ $.N m.$ $.m 2m o.m o.m ©.$ him o.$ 9N o.m $.$ >.$ 0d 2m >.$ $.$ $.$ Em $.$ m.$ ©.$ m.$ >.$ ©.$ m.$ w.$ mim >.$ m.m >.$ >.m m.m o.m Hmsoflc8>coD $.$ 8m 1m o.$ m.$ o.$ $.m o.m 1m ..Hmfipmm 888G 8:828 >u3588h 888G 888C 8.3.888 noHoU Hoe/8:“ 880u< wcflooU 1H8UC8H 853.88% tumom tfiogh Mo 80:82 .mpogu8E mcgooo 026. new mcofimofim8n x8 no“ 88383. c8>8m mo m8uoom 3.258888% 8mmu8>< .MH 838R. 89 Table 14. Average press fluid yields and shear force values of cooked samples for six replications for two cooking methods. Press Fluid Shear Force Method of Cooking (70) (1b.) Radial-conventional 44.631 19.58 7' 41.00 19.01 39.60 18.13 42.03 23.90 39.34 16.40 36.18 19.05 Average 40.46 19. 35 Conventional 37.46 12. 62 39.39 18.27 40.97 17.38 38.00 19. 25 35.07 14.22 32.47 21.45 Average 37.23 17.19 1Based on 2 determinations. 2Based on 4 determinations. 90 Table 15. Cooking weight losses for six roasts for two methods of cookery. Method of Raw Cooked Total Total Drip Volatile Cooking Weight Weight Loss Loss Loss Loss (gm.) (gm.) (gm.) (‘70) (‘70) (‘70) Radial- 1247 920 327 26.22 3.69 22.53 conventlonal 1256 936 320 25.48 4.06 21.42 1174 854 320 27.26 2.90 24.36 1179 878 301 25.53 3.31 22.22 1152 872 280 24. 31 3.47 20.83 1209 857 352 29.12 2.81 26. 30 Average 1203 886 317 26.32 3.37 22.94 Conventional 1170 814 356 30.43 2.22 28.21 1294 960 334 25.81 2.09 23.73 1204 846 358 29.73 2.82 26.91 1149 814 335 29.16 2.00 27.15 1131 767 364 32.18 2.21 29.97 1118 745 373 33. 36 1.52 31.84 Average 1178 825 353 30.11 2.14 27.97 91 Table 16. Volume loss for six roasts for two methods of cookery. Method Volume in Milliliters Volume of Raw Cooked Loss Cooking Sample Sample Change (%) Radial- conventional 1110 745 365 32. 88 1238 790 448 36.19 1140 850 290 25.44 1100 800 300 27. 27 1020 770 250 24. 51 1080 700 380 35.19 Average 1115 776 339 30.25 Conventional 1080 725 355 32. 87 1120 890 230 20. 54 1080 728 352 32.59 1125 880 245 21.78 1030 740 290 28.16 1025 665 360 35.12 Average 1077 771 305 28.51 92 Table 17. Determinations of pH for raw and cooked samples and the change in pH for six roasts for two methods of cookery. Method of pH Readings Change Cooking Raw Samples Cooked Samples in pH Radial- conventional 5 . 6 l 5 . 8 0. 2 5.5 5.8 0 3 5.8 6.0 0.2 5.7 5. 0 2 5.6 6.0 0.4 5.6 5.8 0.2 Conventional 5 . 6 5 . 8 0. 2 5 5 5.8 0 3 5.8 6.0 0 2 5 7 5.9 0 2 5 7 5.9 0 2 5.7 5.9 0 2 1Based on 2 readings. 93 Table 18. Changes in the rate of heat penetration for five replications, for three sample depths for the radial-conventional cooking method. Radial-Conventional Method Samples (Min./OC rise) 0 Temperature C Replications Mean 0. 25-inch Depth Initial - 20 .4 .3 .3 .1 .2 .26 20-30 .7 .2 .4 .5 .2 .40 30-40 .6 .5 .6 .6 .3 .52 40-50 1.0 .7 .8 .6 .4 .70 50-60 1.1 1.3 .9 .7 .9 .98 60-70 1.2 2.0 2.0 1.1 1.7 1.60 70-80 1.5 1.5 1.5 1.2 1.7 1.48 0.75-inch Depth Initial-20 .8 1.1 1.1 1.0 1.4 1.08 20-30 .7 .6 4 .4 .6 .54 30-40 .8 .6 6 .5 .6 .62 40-50 1.0 .7 .7 .6 .7 .74 50-60 1.1 .9 .9 .8 .9 .92 60-70 1.3 1.0 1.2 .9 1.1 1.10 70-80 1.4 1.6 1.4 1.5 1.4 1.46 1-inch Depth Initial - 20 .4 .9 .6 .4 1.0 .66 20-30 .6 .7 .4 .5 .8 .60 30-40 .6 .6 .6 .4 .6 .56 40-50 .8 .7 .7 .5 .8 .70 50-60 .1 .8 .7 .5 .7 .76 60-70 .3 .9 .9 .6 1.1 .96 70-80 .5 1.1 1.5 .9 1.4 1.28 94 Table 19. Changes in the rate of heat penetration for five replications, for three sample depths for the conventional cooking method. ConventionalOMethod Samples (Min./ C rise) 0 Temperature C Replications Mean 0.25-inch Depth Initial - 20 .1 .8 .6 1 5.4 1.08 20-30 .1 .9 .5 2 3.7 .74 30-40 .3 .9 .6 2 2.5 .50 40-50 .5 1.3 1.2 .4 5.2 1.04 50-60 .6 1.7 1.4 1.6 5.9 1.18 60-70 .7 1.8 2.6 4.0 9.6 1.92 70 -80 .9 2.6 3.2 8.0 16.4 3.28 0.75-inch Depth Initial-20 .8 .8 1.1 .9 1.5 1.02 20-30 1.1 .9 1.1 .7 1.2 1.00 30-40 .9 1.2 1.2 .7 1.0 1.00 40-50 .8 1.6 1.2 1.0 .7 1.06 50-60 1.2 1.8 2.0 1.1 1.0 1.42 60-70 2.2 2.7 2.2 3.1 1.7 2.38 70-80 2.0 4.1 2.7 4.1 1.6 2.90 1.50-inch Depth (radius) Initial - 20 2.0 2.0 2.1 1.9 3.0 2.20 20-30 1.0 1.2 1.2 .9 1.7 1.20 30-40 1.0 1.2 1.2 .9 1.1 1.08 40-50 1.2 1.3 1.4 1.1 1.4 1.28 50-60 1.4 1.8 1.8 1.6 1.4 1.60 60-70 1.9 2.2 2.1 3.2 1.8 2.24 70 -80 2.5 4.0 3.0 3.8 2.2 3.10 11001.1 USE om 1100121 13511: ONLY. HICHIGRN STQTE UNIV. LIBRQRIES 1 HI I llllllllll 2 31293010708 24