'— ‘3'4 tailitiizofn't-i's-r': 3-5-1 1 o. I" “"‘(’ I'.' THE EFFECTS OF USING ANAPHAGE (D. P. W.) DR SHAVINGS AS LITTERS 0N BODY WEIGHT GAIN, FEED EFFICIENCY, MEAT FLAVOR, CARCASS QUALITY AND UVABILITY 0F BROILER TYPE CHICKS AND TURKEY POULTS Thesis for the Degree of M. S. MICHIGAN STATE UNIVERSITY JOHN F. KASSID 1977 IVCl’Slty V. R A m n U U m m S n mp .hc M . .i‘QQ- id 3 a. oukzwwm . . a. _. a. mm mm NH on an .fl .n n m 22;: 3:22:23.“2:32:m. 22m :32; l" " c-1 . . l 'I N f- N I“. amzmzzzammzmm:.-.cmmmczazmuzmmczZamzma::a shun—unpu:~:-:~w:-:::~:~::H::::::~: umamcamawmng:mmmfimzmmzwau::emmmmmmmmzzmm.z nmmmnmmmnmmmmmmmmnmmm mama...mmmmmmmmmnnmmmmnmmm:3:: uvwv:wovvwvve¢v«IL.ZZvvwvuvwvvvvvwvwvvzvzfl: IV: nannmnnmmnmmmmzmmnn an:2””an"::mmmmnnnmnnmnmnmmmmn NNNNNNNNNNNNNNNNNNNNZNNNNNNNNNNNNNNN-NNNN~N~N§:N: ZZZ—2.::ZZZ__:::_::::::_::::___r_ 222192122:2$3533333533323332$:2$333,33333923:33:22532:3 529:::acaazaccaaaaaaaeeccco22.3.3299:or- grams: a. 3.... ME ...=.. IDNwwraflfiev, .13 FF... 3.3.3.313, ABSTRACT THE EFFECTS OF USING ANAPHAGE (D.P.W.) OR SHAVINGS AS LITTERS ON BODY WEIGHT GAIN, FEED EFFICIENCY, MEAT FLAVOR, CARCASS QUALITY AND LIVABILITY OF BROILER TYPE CHICKS AND TURKEY POULTS BY John F. Kassid Two experiments were conducted to study the effects of using anaphage or shavings as litter material on body weight gain, feed efficiency, meat flavor, carcass quality and livability of broiler type chicks and turkey poults. A total of 700 male broiler type chicks were randomly assigned among four floor pen units (4.5 m x 3 m) with 175 birds per pen in the first experiment, which was conducted over a period of seven weeks. With the exception of one pen, which had no litter, each of the experimental pens contained a different kind or amount of floor litter. The litters tested were none, 4.4 cm of shavings (control), 4.4 cm of anaphage, and 8.8 cm of anaphage. All experimental groups received the same diet. Chicken starter and chicken grower rations were fed for the first five weeks and the last two weeks, respectively. *1 L) F? John F. Kassid Individual bird weights were taken at the begin- ning and the end of the first experiment. Feed consump- tion was determined for each pen of birds at the time of weighing. The second experiment was carried out over a period of 16 weeks. A total of 360 turkey poults were randomized into eight floor pen units (4.5 m x 3 m) with 45 turkeys in each. Sexes were intermingled. This experiment in- cluded four experimental treatments with two replications of each. I The litters tested were 4.4 cm of anaphage, 2.2 cm of anaphage, 4.4 cm of shavings and a bare concrete floor. All experimental groups received the same diet. Turkey starter and turkey grower were used for the first eight weeks and the second eight weeks of the experiment, respectively. All turkeys in each pen were wing banded and weighed at the beginning of the experiment and at the end of every four weeks period. The feed consumption was measured at the time of weighing turkeys. Taste panels of 1% and 10 judges evaluated the meat flavor of the broilers and turkeys, respectively. A comparison of the data obtained at seven weeks of age in the first experiment shows that broilers raised in the pens with litter weighted significantly more (p < 0.01) than those grown in the pen with no litter. John F. Kassid Broilers raised in the pen with shavings weighed signif- icantly more (p < 0.01) than those grown in the pens with 4.4 cm, and 8.8 cm of anaphage. Broilers raised in the pen with 8.8 cm of anaphage weighed significantly more (p < 0.01) than those grown in the pen with 4.4 cm of anaphage. Livability for all broilers was 96.3% with no significant differences between treatments. Feed efficiencies were 2.43, 2.23, 2.27 and 2.50 for broilers reared on no litter, 4.4 cm of shavings, 8.8 cm of anaphage and 4.4 cm of anaphage, respectively. Analysis of variance of meat flavor of the chickens indicated that broilers raised in the pen with 8.8 cm of anaphage had significantly higher scores (p < 0.05) than those raised intflmapens with no litter, 4.4 cm of shavings and 4.4 cm of anaphage. Breast blister incidences and lowering of U.S. quality of broilers raised on 4.4 cm of anaphage and no litter floor were more pronounced than in those grown on 8.8 cm of anaphage and 4.4 cm of shavings. The data obtained in the second experiment do not show any significant differences in body weight gains, feed efficiency, or mortality between treatments. This was true for both male and female turkeys at 4, 8, 12 and 16 weeks of age. John F. Kassid Taste panelists were unable to detect significnat differences (p > 0.05) in intensity of flavor, acceptance of flavor and general acceptance for light and dark meat of male and female turkey poults reared under the four litter treatments. Although it was not measured, it was obvious that anaphage litter condition in the first and the second experiment was not satisfactory, because of its undesir- able odor, wetness and dustiness, especially at the time of stirring the litter. THE EFFECTS OF USING ANAPHAGE (D.P.W.) OR SHAVINGS AS LITTERS ON BODY WEIGHT GAIN, FEED EFFICIENCY, MEAT FLAVOR, CARCASS QUALITY AND LIVABILITY OF BROILER TYPE CHICKS AND TURKEY POULTS BY John F. Kassid A THESIS Submitted to Michigan State University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Department of Poultry Science 1977 TO MY FAMILY AND MY WIFE ii ACKNOWLEDGEMENTS The author wishes to express his sincere apprecia- tion and gratitude to Dr. T.H. Coleman, Professor of Poultry Science, for his patience and guidance throughout this study and for his many helpful suggestions with the preparation of this manuscript. The writer is also grateful to Dr. H.C. Zindel, chairman, Dr. C.C. Sheppard, Dr. C.J. Flegal, Dr. L.E. Dawson and Dr. B.J. Marquez, who served on my graduate committee and provided valuable counsel in the conduct of this study. The writer wishes to acknowledge his gratitude to Dr. T.H. Coleman, Dr. C.J. Flegal, Dr. C.C. Sheppard and Dr. B.J. Marquez for their help and their efforts in directing this research. The author is grateful to Dr. T.H. Coleman, Dr. C.J. Flegal and Dr. B.J. Marquez for their constructive review of this manuscript.. Special thanks go to Dr. J.L. Gill and Dr. L.E. Dawson for their continuous help during this study. The writer also wishes to express his sincere appreciation to other faculty members of the Department iii Peultry Science, Dr. T. S. Chang,. Dr. D. Polin and Dr. R. K. Ringer for their interest and assistance. The writer expresses gratitude to the government of Iraq for the generous financial support throughout the author's study at Michigan State University. Special thanks also go to my friends and the graduate students in the Poultry Science Department for their help on the farm, the services of the staff at the Poultry Science Research and Teaching Center, and Michigan State University, Department of Poultry Science for the use of the farm.facilities. Last but not least, the writer wishes to express his most sincere appreciation, gratitude and indebted- ness to his parents for their unwavering support and en- couragement throughout his educational career. The author extends his thanks to his wife, Shatha, for her cooperation, patience, devotion and sacrifices which helped make his graduate study possible. I iv TABLE OF CONTENTS Page List of Tables . . . . . . . . . . . . . . . . . . vi List of Appendices . . . . . . . . . . . . . . . . viii Introduction . . . . . . . . . . . . . . . . . . . 1 Review of Literature . . . . . . . . . . . . . . . 3 Procedure . . . . . . . . . . . . . . . . . . . . . 10 Results and Discussion . . . . . . . . . . . . . . 17 Summary and Conclusions . . . . . . . . . . . . . . 33 Literature Cited . . . . . . . . . . . . . . . . . 36 Appendices . . . . . . . . . . . . . . . . . . . . 41 Table LIST OF TABLES Treatment means + standard error (a = 0.05) for final body weights of broilers at the end of seven weeks of age 0 o o o o o o o o o o o o o o o o o o 0 Treatment means : standard error (a = 0.05) for the flavor of the broiler meat I O C O O O O O O O O O O 0 O O O O 0 Results of carcass quality standards of the broilers used in the first experiment . Mean body weight gain (gm) + standard error (a = 0.05) for body of male turkeys at 4 weeks, 8 weeks, 12 weeks and 16 weeks of age . . . . . . . . . . . . . . . . . . Mean body weight gain (gm) : standard error (a = 0.05) for body of female turkeys at 4 weeks, 8 weeks, 12 weeks and 16 weeks of age . . . . . . . . . . . . . . Treatment means : standard error (a = 0.05) for feed efficiency at 4, 8, 12 and 16 weeks of age for turkeys raised on different litter treatments . . . . . . . . Treatment means 1 standard error (a = 0.05) for intensity of flavor of turkey meat, acceptance of flavor, general acceptance (juiciness and tenderness) for light meat of male turkeys . . . . . . . . Treatment means : standard error (a = 0.05) for intensity of flavor of turkey meat, acceptance of flavor, general acceptance (juiciness and tenderness) for dark meat of male turkeys . . . . . . . . . vi Page 24 24 26 27 28 29 30 Table 10 Page Treatment means + standard error (a = 0.05) for ifitensity of flavor of turkey meat, acceptance of flavor, general acceptance (juiciness and tenderness) for light meat of female turkeys . . . . . . . 31 Treatment means : standard error (a = 0.05) for intensity of flavor of turkey meat, acceptance of flavor, general acceptance (juiciness and tenderness) for dark meat of female turkeys . . . . . . . 32 vii LIST OF APPENDICES Appendix Page 1 Composition of the chicken starter ration fed (0-5 weeks of age) in percentage . . . 41 2 Nutrient composition of the chicken starter ration based on calculated analysis . . . . . . . . . . . .'. . . . . 42 3 Composition of the chicken grower ration fed (6-7 weeks of age) in percentage . . . 43 4 Nutrient composition of the chicken grower ration based on calculated analysis . . . . . . . . . . . . . . . . . 44 5 Composition of the turkey starter ration fed (0-8 weeks of age) in percentage . . . 45 6 Nutrient composition of the turkey starter ration based on calculated analysis . . . . . . . . . . . . . . . . . 46 7 Composition of the turkey grower ration fed (8-16 weeks of age) in percentage . . 47 8 Nutrient composition of the turkey grower ration based on calculated analysis . . . 48 9 Sensory evaluation of turkey meat . . . . 49 10 Sensory evaluation of broiler meat . . . . 50 11 Analysis of variance of.final body weight gain of broilers at 7 weeks of age . . . . 51 12 Analysis of variance of chicken meat flavor of the broilers . . . . . . . . . . 51 13 Analysis of variance of body weight gain of male turkeys at 4 weeks of age . . . . 52 viii Appendix Page 14 Analysis of variance of body weight gain of male turkeys at 8 weeks of age . . . . . 52 15 Analysis of variance of body weight gain of male turkeys at 12 weeks of age . . . . 53 16 Analysis of variance of body weight gain of male turkeys at 16 weeks of age . . . . 53 17 Analysis of variance of body weight gain of female turkeys at 4 weeks of age . . . . 54 18 Analysis of variance of body weight gain of female turkeys at 8 weeks of age . . . . 54 19 Analysis of variance of body weight gain of female turkeys at 12 weeks of age . . . 55 20 Analysis of variance of body weight gain of female turkeys at 16 weeks of age . . . 55 21 Analysis of variance of taste panel scores for intensity of flavor of light meat of male turkeys slaughtered at 16 weeks of age . . . . . . . . . . . . . . . . . . . . 56 22 Analysis of variance of taste panel scores for intensity of flavor of dark meat of male turkeys slaughtered at 16 weeks of age . . . . . . . . . . . . . . . . . . . . 56 23 Analysis of variance of taste panel scores for intensity of flavor of light meat of female turkeys slaughtered at 16 weeks of age . . . . . . . . . . . . . . . . . . . . 57 24 Analysis of variance of taste panel scores for intensity of flavor of dark meat of female turkeys slaughtered at 16 weeks of age . . . . . . . . . . . . . . . . . . . . 57 25 Analysis of variance of taste panel scores for light meat flavor of male turkeys slaughtered at 16 weeks of age . . . . . . 58 26 Analysis of variance of taste panel scores for dark meat flavor of male turkeys slaughtered at 16 weeks of age . . . . . . 58 ix Appendix 27 28 29 30 Analysis of variance of taste panel scores for light meat flavor of female turkeys slaughtered at 16 weeks of age . . . . . . Analysis of variance of taste panel scores for dark meat flavor of female turkeys slaughtered at 16 weeks of age . . . . . . Analysis of variance of taste panel general acceptance scores (juiciness and tenderness) for light meat of male turkeys slaughtered at 16 weeks of age . . . . . . Analysis of variance of taste panel (general acceptance scores (juiciness and 31 32 33 34 35 36 37 tenderness) for dark meat of male turkeys slaughtered at 16 weeks of age . . . . . . Analysis of variance of taste panel general acceptance scores (juiciness and tenderness) for light meat of female turkeys slaughtered at 16 weeks of age . . . . . . . . . . . . Analysis of variance of taste panel general acceptance scores (juiciness and tenderness) for dark meat of female turkeys slaughtered at 16 weeks of age . . . . . . . . . . . . Analysis of variance of feed efficiency of turkeys at 4 weeks of age . . . . . . . . Analysis of variance of the feed efficiency of turkeys at 8 weeks of age . . . . . . . Analysis of variance of feed efficiency of turkeys at 12 weeks of age . . . . . . . . Analysis of variance of feed efficiency of turkeys at 16 weeks of age . . . . . . . . Analysis offvariance of mortality from 1 day to 16 weeks of age for all the experi- mental groups of the turkeys used in the experiment . . . . . . . . . . . . . . . . Page 59 59 6O 60 61 61 62 62 63 63 64 INTRODUCTION Floor litter in poultry houses has been used for many years to provide a more desirable environment for growing birds. Many different materials with wide vari- ations in physical properties can be classified as litter. Many materials used as litter in the poultry industry are by-products of other industries. The most popular litter materials in many areas are straw, shavings, ground or crushed corn cobs, peat moss, shredded corn stalks, shredded sugar cane, oat hulls, sawdust, pine bark, rice hulls, can bagasse, hay, peanut shells, cotton seed hulls, sand and mineral litters. Mate- rials used are usually ones that are available locally and are fairly inexpensive. Considerable research effort by many investigators during the past 30 years has been directed toward studying the effect of using many different new litter materials on the performance of birds raised on these litters. To gain additional information, two trials were performed at Michigan State University, Poultry Science Department Research and Teaching Center. Broiler-type chicks and turkey poults were used in the first and second trial, respectively. v l The first experiment was conducted January 24 through March 14, 1975, and the second experiment was carried out during the period starting April 17 and end- ing July 31, 1975. The purpose of the studies reported herein was to further evaluate the effect of using Anaphage (dry poultry waste) as a litter material versus the effect of shavings on the growth, feed efficiency, livability, meat flavor and carcass quality of broilers and turkeys raised on such litters. REVIEW OF LITERATURE 'Considerable research effort has revolved around finding, for growing birds, a floor litter material which can meet the following characteristics: 1. Readily available 2. Cheap 3. Does not pack readily 4. Absorbent 5. Well adapted to use as deep or built-up litterl 6. Fine in texture 7. Free of dust 8. Low fire hazard 9. Light in weight A review of the literature revealed no research reports on raising birds on Anaphage.* However, several workers have reported on the effects of raising birds on different kinds of litter. * A scientific descriptive term for dehydrated poultry waste (D.P.W.). This term was originaged by Dr. D. Polin (personal communication). ‘ ‘ 1Built-up litter means continued rearing of successive groups of young chickens on the same litter without any change. . 3 Seeger gt 31 (1951) reported that on a comparative study of 12 litter materials where production, mortality, feed efficiency, average final weight and pounds of broiler meat produced per chick started were studied, peanut shells head the list as the best litter. The other litters examined were ranked in the following order: corn cobs, peat moss, sugar cane, fiber, sawdust, shavings, cotton seed hulls, corn stalks, Dryzon,2 sand, chick bed,3 and Georgia mineral.4 These investigators also demon- strated that litter, which is of organic origin, is the best for broiler production. Research conducted at North Carolina, Oregon State, Louisiana State, Texas A and M and Purdue Universities has shown that pine bark is equal or somewhat superior to shavings and other materials tested as a litter for both broilers and started pullets. Smith (1956) reported that larger numbers of breast blisters were found on broilers when flocks were raised on wet litter as compared with flocks where the litter was dry. He also stated that litter condition con- tributed a greater effect on breast blister incidence than did kind of litter. Smith (1956), Gyles 33 El. (1961, 1962) and Wisman 22.3l' (1961) demonstrated that management factors such as type of feeder, the type and depth of litter and 2'3'4Types of mineral litter. floor space influence the incidence of breast blisters. Anderson and Cramer (1964) reported that litter moisture content, carbon dioxide and ammonia in the pens increased with the age of the bird. The foregoing experi- mental evidence, particularly with reference to the litter wetness, was further corroborated by the experiment con- ducted by May and Noles (1965). These investigators demonstrated that wet litter increased the incidence of breast blisters. Wesley and Weaver (1974) agreed with Smith's. observation (1956). In their experiment concerning the litter condition, they reported that litter condition, ammonia levels, house temperature and ventilation capacities contributed strongly to the incidence of breast blisters in broilers. Oliveria (1974) reported that inCidence of breast blisters was more pronounced in the case of broilers raised on sand than in those grown on other litters. She also stated that birds reared on wood shavings and rice hulls had less incidences of breast blisters than birds reared on chipped corn cobs, hay, and cane bagasse and that the particle size was an important consideration in selecting a litter for broilers. Aho gt_al. (1955) reported that when used as litter, wood chips of one to two inches in circumference resulted in drier conditions than wood chips three or four inches in circumference. Ruzler and Carson (1974) stated that cane pomace heads a list of five broiler litter materials in its ability to absorb moisture. It was followed by wood shavings, crushed corn cobs, peanut shells and pine bark. Abbott 23 El. (1969) reported that the occurrence of foot-pad dermatitis in turkey poults was related to the wetness and crustiness of the litter used. Ross (1968) reported that chicks reared on fresh litter have slightly lower mortality than those reared on reused litter. Unlike Ross (1968), Reed and McCartney (1970) reported that there was no difference in mortality of broilers reared on reused litter and those reared on new litter. Many experiments were conducted to study the effect of using built-up litter in poultry houses on the performance of birds. Kennard and Chamberlin (1950) stated that better growth, feed efficiency and viability were found in many cases when chickens were raised on old built-up litter. It was reported by Kennard and Chamberlin (1949) that using built-up litter in poultry houses was con- sidered a new type of sanitation, in which the action of the litter works against harmful bacteria, protozoa and other disease-producing factors. With the development of built-up litter came the treatment of litter with hydrated lime. Kennard and Chamberlin (1947, 1948b,c) reported that adding hydrated lime to floor litter aided in keeping litter more friable and absorbent and less inclined to pastecnrcake over the surface. Kennard, Bethke and Chamberlin (1948a) reported that built-up litter was a potent source of valuable nutrients. Some evidence is found in the literature indicat- ing that litter management may affect the amount of nutrients required in the diet. Lamoreux and Schumacher (1940) stated that hen feces contained more riboflavin than the amount in the feed which was fed. Schumacher and Heuser (1941) isolated an organism capable of synthesizing riboflavin in hen feces. Rubin gt a1. (1946) observed that the animal pro- tein factor was present in hen feces. McGinnis 23 21. (1947) found that incubating hen feces for 72 hours at 30°C stimulated further synthesis of the unidentified factor.necessary for growth. Halbrook (1950) isolated from built-up litter 140 different organisms, which were capable of synthesizing vitamin B12. Halbrook gt_al. (1951) Stated that "there was a definite trend for bacteria, molds and yeasts to increase in poultry house litter with age up to at least 8 weeks." They also showed that the change in pH of litter with increasing age probably explains the decrease in yeasts and molds present in built-up litter. Various attempts have been made to alter or control the microbial population of litter. Halbrook gt_al. (1951) reported that the use of quick lime in built-up litter reduced noticeably all classes of bacteria, yeasts and molds. Andrews gt El° (1943), Swanson and Taylor (1943), Boney (1948) and Edgar and King (1955), utilized methyl bromide for the sterilization of parasite contaminated soil or litter. Some investigators worked on the effect of insecticide impregnated litters on the performance of broilers and on the processed products from such treated poultry. Tower and Floyd (1961) reported that "the perfor- mance of chicks brooded and reared on sugarcane bagasse litter that had been impregnated with Lindane was not affected." “ They also reported that "there was no difference in consumer acceptance between birds grown on Lindane treated litter and those grown on untreated litter." A review of the literature revealed that some work has been done by several investigators on the utilization of poultry litter as a nitrogen source in the diet of livestock (Noland §t_aln, 1955; Southwell £5 31., 1958; Camp, 1959; Carmody, 1964; Brugman gt 21,, 1964, Fontenot g 511., 1964 and 1965; Ray and Child, 1964, 1965; Bahttacharya, A.N. and J.P. Fontenot, l965a,b; and Drake 22 31., 1965). The inclusion of animal and poultry waste in the rations of poultry has also been reported by Rubin 33 31., 1946; Palafox and Rosenberg, 1951; Elam 33 21., 1954; Wehunt st 31., 1960; Durham st 31., 1966; Camp, 1967; Quisenberry and Bradley, 1968; and Flegal and Zindel, 1970. Poultry house litter has been shown to be high in crude protein and low in energy, vitamin A, and vitamin D as reported by Brugman gt El. (1964). PROCEDURE Two experiments were performed to investigate the effect of raising broiler-type chicks and turkey poults in floor pen units with two different levels of anaphage (dehydrated poultry waste), shavings, or no litter on the floor on body weight gain, feed efficiency, meat flavor, carcass quality and livability. The first experiment was conducted over a period of seven weeks starting January 24 and ending March 14, 1975. The housing facilities consisted of four floor pens (4.5 m x 3 m), which were thoroughly cleaned and dis- infected. The pens were separated from each other by wire netting partitions. Each pen housed 175 males (Hubbard strain one-day- old commercially hatched broiler type chicks). With the exception of one pen, which was a bare concrete floor with no litter, each of the experimental pens contained a different kind or amount of floor litter. The litters tested were none, 4.4 cm of shavings (used as a standard for comparison), 4.4 cm of anaphage, and 8.8 cm of anaphage. _ 10 11 Gas heated brooders were used. For the first two weeks of the experimental period flat type feeders and jar-waterers were employed. These were then replaced by hanging feeders and automatic waterers. Water in the water founts was kept at a certain level to prevent damp- ‘ness of the litter beneath the founts. Attempts were made to provide the maximum.amount of ventilation in keeping with the comfort of the chicks and the weather condition. All experimental groups received the same diet. During the first five weeks, a chicken starter ration was fed (Appendix table 1), and for the last two weeks of the experiment, a chicken grower ration was fed (Appendix table 3). The feed was weighed at the beginning and the end of the experimental period. Feed consumption and mortality were recorded. Chicken starter and chicken grower rations were adequate in all known nutrients required in commercial broilers based on calculated analysis (Appendix tables 2 and 4). .All the broilers were individually weighed at the beginning and at the end of the experimental period. At the beginning of the experiment 50 broiler type chicks from each pen were banded for statistical purposes. The 12 birds were vaccinated twice against Newcastle and Bron- chitis by the drinking water method on the second and the fourth week of the experimental period. At the end of the experiment 25 broilers from each pen were slaughtered for the purpose of carcass quality evaluation. A taste panel of 20 judges from students, faculty and other employees was employed at the Food Science De- partment, Michigan State University to evaluate the flavor and acceptability of the meat produced on the four litter regimes. The second experiment was carried out over a period of 16 weeks starting April 17, 1975 and ending July 31, 1975. A total of 360 turkeys (one-day-old poults) were randomized into eight floor pen units (4.5 cm x 3 m) with 45 turkeys in each. Sexes were intermingled. This experiment included four experimental treatments, with two replications of each. The litters tested were 4.4 cm of anaphage, 2.2 cm of anaphage, 4.4 cm of shavings (used as a standard for comparison) and a bare concrete floor with no litter on it. Gas heated hover type brooders and electric heat bulbs were used to provide the temperature needed during the brooding period. Brooder guards were used for seven days. For the first three weeks of the experimental period, flat type feeders and jar-waterers were employed. 13 At the beginning of the fourth week they were replaced by hanging feeders and automatic waterers. All experimental groups received the same diet. For the first eight weeks, a turkey starter ration was .fed (Appendix table 5), then for the second eight weeks of the experiment, a turkey grower ration was fed (Appendix table 7). In the turkey experiment feed was weighed at the beginning and the end of every four week period. Feed consumption and mortality were recorded. Turkey starter and turkey grower ration were adequate in all known nu- trients required in turkey production, based on calculated analysis (Appendix tables 6 and 8). All turkeys were banded and were individually weighed at one day of age and at the end of every four week period up to 16 weeks of age. Turkeys were medicated by the use of Tylan at the beginning of 4, 12 and 16 weeks of age. One male and one female from each experimental group was slaughtered at the end of the experiment for the purpose of meat evaluation. One of the slaughtered females which was taken from the 2.2 cm of anaphage treatment was lost during the refrigeration time, so an analysis of variance test was completed for the rest of the saved turkeys. A taste panel of 12 judges from students, faculty and other employees was employed at the Food Science Department, 14 Michigan State University for the purpose of evaluating flavor of the meat produced on the four litter treatments. In both taste panels (i.e., for broilers and turkeys), panel members were instructed to evaluate the meat on the basis of: 1. Like extremely 2. Like very much 3. Like moderately 4. Like slightly 5. Neither like nor dislike 6. Dislike slightly 7. Dislike moderately 8. Dislike very much 9. Dislike extremely, (Appendix tables 9 and 12) The scores given by panel members ranged from (1) to (9) for like extremely and dislike extremely, respec- tively. Results of the taste dfifference test for both experiments were subjected to analysis by the Hedonic Rating System, as described in the Merck Technical Bul— letin (1963). The analysis of variance for the following char- acteristics was obtained: 1. Final average body weight gain for the broiler type chicks and turkeys (Appendix ll, 13, 14, 15, 16, 17, 18, 19 and 20). 15 2. Chicken meat flavor at the end of seven weeks (Appendix 12). 3. Feed conversion of turkeys at 4, 8, 12 and 16 weeks of age for both Toms and hens (combined Appendix 33, 34, 35 and 36, respectively). Feed conversion was done on the assumption that males and females were in equal percentages because distribution of birds into each pen was random. 4. Intensity of turkey flavor, acceptability of flavor, general acceptance (juiciness and tenderness) for light and dark meat of tur- key males and females at 16 weeks of age (Appendix 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31 and 32). 5. Mortality from the period of one day to 16 weeks of age for all experimental groups of turkeys used in the experiment (Appendix 37). The pen means of the study were evaluated statis- tically by the analysis of variance using the F-ration as a measure of significance., If the F-ratio was significant, orthogonal contrasts were employed to determine which treatments were significantly different at the 0.05 and 0.01 level of probability. Final average body weights, and individual body weight gains are expressed as the ratio of feed intake to the body weight gain. 16 Mortality is expressed as a percent of the total chicks housed at the beginning of the experiment. In both experiments, the litter was thoroughly stirred and redistributed at frequent intervals, to prevent packing and caking over the surface, and especially around the feeders and water founts. Dead birds from both experi- ments were removed from the pens and sent to the labora- tory for determining the cause of death. RESULTS AND DISCUSSION Experiment One - Broiler Type Chicks Final average body weight gain (individual weights). Analysis of variance of final body weight gain of broilers used in the experiment at the end of seven weeks was conducted (Appendix 11), the results were as follows. Broilers raised in the pens with litter weighed significantly more (p < 0.01) than those grown in the pen with no litter (Table 1). This suggests that litter pro- vides a more desirable environment for growing birds in poultry houses than does a bare floor. Broilers raised in the pen with shavings weighed significantly more (p < 0.01) than those grown in the pens with 4.4 cm and 8.8 cm of anaphage (Table 1). This result agrees with the general opinion that shavings are one of the best litter materials to use in poultry houses. Broilers raised in the pen with 8.8 cm of anaphage weighed signif- icantly more (p < 0.01) than those grown in the pen with 4.4 cm of anaphage (Table 1). It is possible that 8.8 cm of anaphage absorbs more moisture than 4.4 cm of anaphage which aids in providing a better bedding situa- tion for growing birds. 17 18 At the beginning of the experiment 50 broiler type chicks from each pen were banded for the purpose of running a regression analysis of final body weights on initial body weights of the broilers. The reasons for running a regression analysis was to test if final body weight is related to initial body weight and, if related, to adjust treatment means of final body weight for dif- ference in mean initial weight. The idea is to compare treatment means as if all groups began with the same mean initial weight. The results showed that final body weight was correlated to initial body weight. When the treat- ment means of the final body weights of the broilers were adjusted to the differences in the initial body weight means at the time when the birds were housed in the pens, the same results were obtained, i.e., the relative differences among treatment means were only trivially altered by the adjustment for differences in initial weight. Feed efficiency. Feed efficiencies to seven weeks of age were 2.43, 2.23, 2.27 and 2.50 for broilers grown in pens with no litter, shavings (control), 8.8 cm of anaphage, and 4.4 cm of anaphage, respectively. These figures are not an absolutely correct estimation of the feed efficiency, because we had only one replication of each treatment, and probably they could be affected by many different environmental conditions. 19 Meat flavor. Analysis of variance of chicken meat flavor of the broilers used in the experiment was conducted at the end of the seven week test (Appendix 12). The re- sult indicated that broilers raised in the pen with 8.8 cm of anaphage tasted significnatly better (p < 0.05) than those raised in the pens with no litter, 4.4 cm of shavings and 4.4 cm of anaphage. On the other hand, orthogonal contrasts indicated that no one of the other experimental groups was signif- icantly better in acceptability of the meat flavor than the other (Table 2). Carcass quality. .U.S. quality, breast blister and bruising (discolored skin) were the only three criteria of the carcass quality standards that were studied. Re- sults are shown in Table 3. Briefly summarized, the results of the carcass quality study show that breast blister incidence of broilers raised on 4.4 cm of anaphage and no litter floor was greater than in those grown on 8.8 cm of anaphage and shavings. This agrees with the observations of Smith (1956); May and Noles (1965); Glyes gt 31., (1961); Wisman gt_al., (1961); and Wesley and Weaver (1974) that type and depth I of litter and litter conditions influence the breast blister incidence. 20 As far as U.S. Quality is concerned, all broilers raised on shavings and 8.8 cm of anaphage were of grade 1 A-quality , while some of those raised on 4.4 cm of anaphage and no litter floor were of grade B-quality2 with slight breast defect. Differences among the treatments as far as bruising was concerned were probably due to handling the birds before slaughtering them or to management condi— tions as reported by Ringrose (1953); Kaiser and Smith (1958), and Handy gt 21., 1961. Livability. Livability percent of the different experimental groups was almost the same. Mortality percentage was negligible for all broilers up to seven weeks of age. It was 3.7 percent. This will indicate that mortality percentages among the treatment groups were not affected by type of litter used. Experiment Two - Turkey Poults Body weight gains. Body weight gain of male and female turkeys at 4, 8, 12 and 16 weeks of age for shav- ings, no litter, 2.2 cm of anaphage and 4.4 cm of anaphage are shown in Table 4 and 5. 1A-quality means no obvious breast defect. 2B-quality means slight breast defect sufficient to lower grade. 21 There is very little evidence that the treatment groups differed in mean body weight gain. This was true for females and males at 4, 8, 12 and 16 weeks of age (Appendix 13, 14, 15, l6, 17, 18, 19 and 20). Unlike what was found with Broiler type chicks, the data obtained here showed that using anaphage and shavings as litter materials has no effect on body weight gain of turkey poults. Feed efficiency. Feed efficiencies of the tur- keys used in the experiment at 4, 8, 12 and 16 weeks of age for shavings, no litter, 2.2 cm of anaphage and 4.4 cm of anaphage were illustrated in Table 6. Analysis of variance of feed efficiency of the turkeys used in the experiment at 4, 8, 12 and 16 weeks of age was conducted. There was no evidence of signif- icant difference (p > 0.05) between the experimental groups (Appendix 33, 34, 35 and 36). The feed efficiency of turkeys grown on anaphage and shavings was approximately equal. Meat flavor. Evaluation of intensity of flavor of turkey meat, acceptability of flavor, general accep- tance (juiciness and tenderness) for light and dark meat of male and female turkeys grown on shavings, no litter, 2.2 cm of anaphage or 4.4 cm of anaphage are shown in Tables 7, 8, 9 and 10. 22 Analysis of variance of intensity of turkey flavor, acceptance of flavor, general acceptance (juici- ness and tenderness) for light and dark meat of male and female turkeys at 16 weeks of age were conducted. Taste panelists were unable to detect signif- icant differnece (p > 0.05) in intensity of flavor, acceptance of flavor and general acceptance for light and dark meat of males and females of turkeys reared under the four litter treatments (Appendix 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31 and 32). Livability. Mortality was 10.8% for the period of 16 weeks (from 1 day of age to 16 weeks of age) for all the experimental groups. According to the F-test, there were no signif- icant differences in mortality between the different experimental groups (Appendix 37). From looking at all the data which were obtained from the second experiment, no significant difference was found in body weight gains, feed efficiency or mortality between treatments. This was true for both male and female turkeys at.4, 8, 12 and 16 weeks of age. The only conclusion that could be derived from these data would be that dehydrated poultry waste (anaphage) used in this trial was somewhat equal to shavings as a litter material. 23 It is possible that management practices and ventilation would have a greater effect on the perfor- mance of turkey poults than the kind of floor litter. This needs to be substantiated by further tests. In addition to the results obtained in both trials, there were some other things which were not measured (i.e., dust level, litter moisture condition and odor). An unpleasant odor and an increased level of dust were some of the problems which were encountered in the pens with anaphage as the litter. Increased litter moisture was the other problem which existed in the pens utilizing anaphage as a litter in both trials in spite of all the attempts which were done to prevent dampness and wetness of the litter. Anaphage litter condition in the first and the second experiment was not satisfactory because of its undesirable odor, wetness and dustiness, especially at the time of stirring the litter. 24 Table 1. Treatment means : standard error (a = 0.05) for final body weights of Broilers at the end of 7 weeks of age. Treatment Mean body weight (gm) i_standard error 4'4 cm °f 1676 + 25.9c anaphage — 8'8 cm °f 1745 + 25.9b anaphage — . be No litter 1699 :_25.7 Shavings a control 1793.i 25.5 Values with different superscript differ significantly, p < 0.01. Note: Only males were used in the first experiment. Table 2. Treatment means : standard error (a = 0.05) for the flavor of broiler meat. Treatment Scores of meat flavor i standard error No litter 7.15 i .415 4.4 cm of anaphage 6.8 i .415 Shavings .. 6.75 i .415 8.8 cm of 7.41 + .415 anaphage - Note: The scores were ranged frOm like extremely (9) to- dislike extremely (1). 1Significantly better (p < 0.05) than other experimental group. 25 Table 3. Results of carcass quality standards of the broilers used in the first experiment. 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H $8 as; H 8;. 34 H co... 3 mortgage Ammmcuwocoa can mmwcHOHsov Ho>mHm Ho>mam mocmummoom Hmnmcmo mo mosmummood hexane mo auHmcmusH unmaummua .mhmxusu wamfimm mo Home xumo How Ammmcuwocwu can mmmcHOHsmv mocmummoom Hmumcwm .HO>MHM mo mocmufiwoom .ume mmxusu mo Ho>mam mo muHmcmucH How Amo.o u ov Honuo onmocmum + momma usmfiummua .OH manna SUMMARY AND-CONCLUSION First Experiment BrOilers raised in the pens with litter weighed significantly more (p < 0.01) than those grown in the pen with no litter. Broilers grown in the pen with shavings weighed significantly more (p < 0.01) than those grown in the pens with 4.4 cm, and 8.8 cm of anaphage. Broilers raised in the pen with 8.8 cm of anaphage weighed signif- icantly more (p < 0.01) than those grown in the pen with 4.4 cm of anaphage. Feed efficiencies to seven weeks of age were 2.43, 2.23, 2.27 and 2.50 for broilers raised on no litter floor, shavings, 8.8 cm of anaphage and 4.4 cm of anaphage, respectively. Broilers raised in the pen with 8.8 cm of anaphage tasted significantly better (p < 0.05) than those raised in the pens with no litter, 4.4 cm of shavings and 4.4 cm of anaphage. The breast blister incidence of broilers raised on 4.4 cm of anaphage and no litter floor was greater than that of those grown on 8.8 cm of anaphage and shavings. All the broilers raised on shavings and 8.8 cm of anaphage were of grade A quality with no obvious breast blister defect, while some of those broilers that were 33 34 raised on 4.4 cm of anaphage and no litter floor were B quality with slight breast defect. Differences between treatments as far as bruising is concerned were probably due to handling and management conditions before slaughtering the broilers. Mortality percentage for all broilers up to seven weeks of age was 3.7%. Second Experiment The data obtained in the second experiment do not suggest any significant differences in body weight gains, feed efficiency or mortality among treatments, and this was true for male and female turkeys at 4, 8, 12 and 16 weeks of age. Taste panelists were unable to detect significant differences (p > 0.05) in intensity of flavor, acceptance of flavor and general acceptance for light and dark meat of males and females of turkeys reared under the floor litter treatments. The only conclusion that could be derived from these data would be that dehydrated poultry waste (anaphage) used in this trial was somewhat equal to shavings as a litter material. It is possible that management practices and ventilation would have a greater effect on the performance of turkey poults than the kind of floor litter. This needs to be substantiated by further tests. 35 Although it was not measured, it was obvious that anaphage litter condition in the first and the second experiment was not satisfactory because of its undesirable odor, wetness and dustiness, especially at the time of stirring the litter. LITERATURE CITED LITERATURE CITED Abbot, W.W., J.R. Couch and R.L. Atkinson, 1969. The incidence of foot-pad dermatitis in young turkeys fed high levels of soybean meal. Poultry Sci. 48: 2186-2188. Aho, W.A., W.A. Junnila and H.C. Wheldon, Jr., 1955. Wood chips for poultry litter. Poultry Sci. 34: 1175. Anderson, D.P. and C.O. Cramer, 1964. Progress report on environmental studies on confinement growing of -turkeys as related to respiratory disease problems. Paper presented to ASAE Annual meeting, Colorado State University, June 21-24, 1964. Andrews, J.A., A.L. Taylor and L.E. Swanson, 1943. Fumigation of soil with methyl bromide as a means of destroying infective stages and intermediate hosts of some internal parasites of mammels. Proc. Helminth. Soc. Wash. 10: 4-6. Bhattacharya, A.N. and J.P. Fontenot, 1965a. Utiliza- tion of different levels of poultry litter nitrogen by sheep. J. Animal Sci. 24: 1174-1178. Bhattacharya, A.N. and J.P. Fontenot, 1965b. Protein and energy value of peanut hull and wood shaving poultry litters. J. Animal Sci. 25: 367-371. Boney, W.A., 1948. The efficacy of methylbromide as a fumigant for the prevention of cecal coccidiosis in chickens. Amer. J. Vet. Res. 9: 210-214. Brugman, H.H., H.C. Dickey, B.B. Plummer and B.R. Poulton, 1964. Nutritive value of poultry litter. J. Animal Sci. 23: 869. Camp, A.A., 1959. Broiler-house litter as livestock feed. Texas Agri. Progr. 5: 17. 36 - 37 Camp, A.A., 1967. The use of processed used poultry house litter in the diet of poultry. West Virginia Poultry Association. Carmody, R., 1964. Chicken litter cow feed. Farm Quart. 19: 52-53, 92, 94. Drake, C.L., W.H. McClure and J.P. Fontenot, 1965. Broiler litter as feed for ruminants. Livestock Research Progress Report, 1964-65. Virginia Agricultural Experiment Station, Blacksburg, Va. Durham, R.M., G.W. Thomas, R.C. Albin, L.G. Howe, S.E. Curl and T.W. Box, 1966. Coprophagy and use of animal waste in livestock feeds. ASAE Publication No. 0366: 112-114. Edgar, S.A. and D.F. King, 1955. The effectiveness of methyl bromide in sterilizing poultry litter. _Poultry Sci. 34: 595-597. Elam, J.F., R.L. Jacobs and J.R. Couch, 1954. Unidentified factor found in autoclaved litter. Poultry Sci. 33: 1053. Flegal, C.J. and H.C. Zindel, 1970. The utilization of poultry waste as a feedstuff.for growing chicks. Research Report No. 117: 21-28. Fontenot, J.P., C.L. Drake, W.H. McClure, F.S. McClaugherty, A.N. Bhattacharya, R.F. Kelly and G.W. Litten, 1964. The value of poultry litter as a feed for ruminants. Livestock Research Progress Report, 1963-64. Virginia Agricultural Experiment Station, Blacksburg, Va. Fontenot, J.P., F.S. McClaugherty and W.H. McClure, 1965. The value of urea in wintering rations for weaning beef calves. Livestock Research Progress Report, 1964-65. Virginia Agricultural Experiment Station, Blacksburg, Va. Gyles, N.R., J. Kan and R.M. Smith, 1962. The heritability of breast blister condition and breast feather coverage in a White Rock Broiler strain. Poultry Sci. 41: 13-16. Gyles, N.R., B.N. McPherson and L.D. Andrews, 1961. The influence of type of feeder on breast blisters in broilers. Poultry Sci. 40: 814-816. 38 Halbrook, B.R., 1950. Vitamin 312 synthesis by micro- organisms in poultry house litter. Ph.D. Thesis, Ohio State University. Halbrook, B.R., A.R. Winter and T.S. Sutton, 1951. The microflora of poultry house litter and droppings. Poultry Sci. 30: 381-388. Hamdy, M.K., K.N. May and J.J. Power, 1961. Some physical and physiological factors affecting poultry bruises. Poultry Sci. 40: 790-795. Kaiser, W.K. and R.C. Smith, 1958. Factors affecting the bruising of broilers. Delaware Agri. Exp. Sta. Bul. No. 323. Kennard, D.C. and V.D. Chamberlin, 1947. Lime treatment of floor litter for chickens. Ohio Agric. Exp. Sta. Farm and Home Research 32: 11-14. Kennard, D.C., R.M. Bethke and V.D. Chamberlin, 1948a. Built-up floor litter as a source of dietary factors essential for hatchability of chicken eggs. Poultry Sci. 27: 477-481. Kennard, D.C. and V.D. Chamberlin, 1948b. Built-up litter as a source of dietary factors essential for the growth of chickens. Poultry Sci. 27: 240-243. Kennard, D.C. and V.D. Chamberlin, 19480. Built-up litter to date. Ohio Agric. Exp. Sta. Farm and Home Re- search 33: 130-135. Kennard, D.C. and V.D. Chamberlin, 1949. Built-up floor litter sanitation and nutrition. Ohio Agric. Exp. Sta. Farm and Home Research 34: 162-166. Kennard, D.C. and V.D. Chamberlin, 1950. Growth, dietary requirements and mortality of chickens as affected by management of the floor litter. Poultry Sci. 29: 766. Lamoreux, W.F. and A.E. Schumacher, 1940. Is riboflavin synthesized in the feces of fowl? Poultry Sci. 25: 486-491. May, K.N. and R.K. Noles, 1965. Effect of hesperdin and litter moisture on growth and carcass defects of broilers. Poultry Sci. 44: 844-848. 39 McGinnis, J., J.M. Stevens and K. Groves, 1947. The in vitro synthesis of a chick growth promoting factor in hen feces. Poultry Sci. 26: 432-433. Merck Technical Bulletin, 1963. An introduction to taste testing of foods. Merck and Co., Inc., Rahway, New Jersey. Noland, P.R., B.F. Ford and M.L. Ray, 1955. The use of ground chicken litter as a source of nitrogen for gestating-lactating cows and fattening steers. J. Animal Sci. 14: 860-865. Oliveria, S.C., 1974. Comparison of litter materials for broiler production. Proc. of XV World's Poultry Congress and Exposition. 342-344. Palafox, A.L. and M.M. Rosenberg, 1951. Dried cow manure as a supplement in a layer and breeder ration. Poultry Sci. 30: 136-142. Quisenberry, J.H. and J.W. Bradley, 1968. Nutrient re- cycling. Second National Poultry Litter and Waste Management Seminar. 96-106. Ray, M.L. and R.D. Child, 1964. He's doing well on broiler house litter. Feed Bag 40: 46-47. Ray, M.L. and R.D. Child, 1965. Chicken litter as a supplement in wintering beef cows and calves on pasture. Arkansas Farm Research, 14: 5. Reed, M.J. and M.G. McCartney, 1970. Physical properties of selected litter materials and performance of broiler chickens. University of Georgia College of Agriculture Expt. Sta. Research Bul. 75. Ringrose, A.T., 1953. Bruised poultry challenges pro- cessor's profits. Am. Egg Poultry Review, 36-39. Ross, E., 1968. Fumigation and reuse of broiler litter. Poultry Sci. 47: 1711. Rubin, M., H.R. Bird and I. Rothchild, 1946. A growth promoting factor for chicks in the feces of hens. Poultry Sci. 25: 526-528. Ruzler, P.L. and J.R. Carson, 1974. Methods of evaluating the potential usefulness of selected litter materials. Poultry Sci. 53: 1420-1427. 40 Schumacher, A.E. and G.F. Heuser, 1941. Isolation of an organism responsible for the increased riboflavin content of the feces of the fowl. Poultry Sci. 20: 272-277. Seeger, R.C., A.E. Tomhave and W.C. Lucas, 1951. A com- parison of litters used for broiler production. Delaware Agri. Expt. Sta. Bul. No. 289. Smith, R.C., 1956. Kind of litter and breast blisters on broilers. Poultry Sci. 35: 593-595. Southwell, B.L., O.M. Hale and W.C. McCormick, 1958. Poultry house litter as a protein supplement in steer fattening rations. Mimeograph Series N.S. 55. Georgia Agricultural Experiment Station, Athens, Ga. Swanson, L.E. and A.L. Taylor, 1943. Control of cattle- parasitic and free-living nematodes by soil fumiga- tion with methyl bromide. Proc. Heminth. Soc. Wash. 10: 1-3. Tower, A.A. and E.H. Floyd, 1961. Consumer and organ- oleptic tests with broilers grown on lindane- impregnated 1itter. Poultry Sci. 40: 234-238. Wehunt, K.E., R.L. Fuller and H.M. Edwards, 1960. The nutritional value of hydrolyzed poultry manure for broiler chicks. Poultry Sci. 39: 1057-1063. Wesley, L.R. and W.D. Weaver, Jr., 1974. Some factors affecting the incidence of breast blisters in com- mercial broilers. Poultry Sci. 53: 1991. Wisman, E.L., W.L. Beane, W.R. Luckman and E.O. Essary, 1961. Efficiency of growing broilers to a heavier weight. Poultry Sci. 40: 1705-1712. APPENDICES 41 Appendix Table 1. Composition of the chicken starter ration fed (0-5 weeks of age) in percentage. Ingredient Percent Corn 62.15 Soybean meal, 49% 20.50 Alfalfa, 17% 2.5 Meat and bone meal, 50% 3.0 Fish meal, 60% 2.5 Whey, dried 2.0 Oats 5.0 Salt 0.25 Limestone, ground 0.75 Dicalcium phOSphate 0.75 Premix (5003) 0.5 Additives 0.1 42 Appendix Table 2. Nutrient composition of the chicken starter ration based on calculated analysis. Nutrient Percent Crude protein 20.00 Fat 3.28 Fiber 3.62 Calcium 1.00 Phosphorus, avail. .50 ME, Cal/Ib 1340.00 43 Appendix Table 3. Composition of the chicken grower ration fed (6-7 weeks of age) in percentage Ingredient Percent Corn 61.8 Soybean meal, 49% 16.25 Alfalfa, 17% V2.5 Meat and bone meal, 50% 3.0 Fish meal, 60% 1.5 Oats 12.5 Salt 0.25 Limestone, ground 0.6 Dicalcium phosphate 1 Premix (5003) 0.5 Additives 0.1 44 Appendix Table 4. Nutrient composition of the chicken . grower ratiOn based on calculated analysis. Nutrient Percent Crude protein 18.00 Fat 3.50 Fiber 4.38 Calcium .91 Phosphorus, avail. .50 ME, Cal/Ib. 1350.00 45 Appendix Table 5. Composition of the turkey starter ration fed (0-8 weeks of age) in percentage Ingredient Percent Corn 42.6466 Soybean meal, 49% 40.9987 Alfalfa meal, 17% 2.9962 Fish meal, 60% 2.9962 Meat and bone meal, 50% 2.9962 Whey, dried ’ 1.997s Fat, Av 1.4981 Salt 0.2496 Dicalcium phosphate 1.4981 Limestone 1.2484 Premix 5004 0.7490 Biotin 0.1248 46 Appendix Table 6. Nutrient composition of the turkey starter ration based on calculated analysis. Nutrient Percent Crude protein 28.00 Fat . 3.89 Fiber 3.29 Calcium 1.40 Phosphorus, avail. .67 ME, Cal/Ib 1263.00 47 Appendix Table 7. Composition of the turkey grower ration fed (8-16 weeks of age) in percentage. Ingredient Percent Corn 58.7677 Soybean meal, 49% 29.8328 Alfalfa meal, 17% 2.4943 Meat and bone meal, 50% 3.4921 Fat, Av . 2.4943 Salt 0.2494 Dicalcium phosphate 1.2471 Limestone 0.7483 Premix 5004 0.5986 Biotin 0.0748 Appendix Table 8. 48 Nutrient composition of the turkey grower ration based on calculated analysis. Nutrient Percent Crude protein 22.00 Fat 5.22 Fiber 3.25 Calcium 1.00 Phosphorus, avail. .55 ME, Cal/1b 1382.00 4S) mmwc mom: who: mmoc lumocwu uuwocmu nuwocwu Iuwocwu .mmw: uo>mam .mmoc uo>mam .mmw: Ho>mHm .mmoc uo>m~m uHoHsn uo>sHm hwxusa IHUHSn uo>mHm >mxusa IHoHsn uo>mHm Hogans IHUHsfl uo>mam auxush ".umwoo< no mo ".ummoo< mo mo ".ummoo¢ wo wo ".umoood no no mumcww .uQmoot SwamcwucH Hmuwcwo .uooooc huwchDCH .Hmuwcoo .umoood hummcoucm Hmumcmu. .umooo< auwmcuucH moou OUOU ooou oooo mama OEQZ hawsouuxo oxaamwo, nose huo> uxHHuHa haouuuovoa oxHHnHa Hangmaao oxaaoao oxaaofio no: axed uoauaoz adorned» oxaq haounuooOE oxHA nose huo> oxuq Samsouuxo axHA you: aoxusa mo :oHuoan>u auoocom .m «Hana awesomm< 50 Appendix Table 10. Sensory Evaluation of Broiler Meat Name: Product: Date: PREFERENCE Code Code Code Code Like Like JLike Like "'I—— ..__.. _— Extremely Extremely Extremely Extremely _Like LDike Like Like "very much _Dike . Moderately _Like LSIightly _Dgither like nor dislike J J Dislike Slightly “Dislike Moderately "Dislike Very much Dislike IEitremely .1 Comments: J Very much 11.1w— 7Moderately _LEiIke S ightly Dgither like nor dislike Dislike Slightly (Dislike Moderately L Dislike Very much Dislike Extremely Comments: qvery much .L_ike Moderately like USlightly iggither like nor dislike Dislike Slightly “Dislike Moderately _Dislike Very much “Dislike Extremely Comments: Very much Like Moderately Like Slightly Neither like nor dislike Dislike Slightly Dislike Moderately Dislike Very much Dislike Extremely Comments: Appendix Table 11. 51 Analysis of variance of final body Weight gain of broilers at 7 weeks 0 f age. gource of Degree of Sum of Mean F variation freedom square square Statistic f.001 Treatments 3 1372693 457564 15.3 5.42* Firds/Trts 671 19431438 28958 * Significant Appendix Table 12. flavor of the broilers Analysis of variance of chicken meat Source of Degree of Sum of Mean F ~ variation freedom square square Statistic f.05 * Treatments 3 5.65 1.88 2.15 2.09 xpt. Error 76 j66.3 .872 fi * Significant f Appendix Table 13. 52 Analysis of variance of body weight gain of male turkeys at 4 weeks of age. ource of Degree of Sum of Mean F ariation freedom square square Statistic f.001 Treatments 3 26892 12297 1.33 5.42 Firds/trts 144 1331093 9243 Appendix Table 14. Analysis of variance of body weight gain of male turkeys at 8 weeks of age 0 Eource of Degree of Sum of Mean F ariation freedom square square Statistic f.001 Treatments 3 740324 246775 1.98 5.42 Birds/trts 144 17926398 124488 Appendix Table 15. 53 Analysis of variance of body weight gain of male turkeys at 12 weeks of age. Source of Degree of Sum of Mean F variation freedom square square Statistic f.001 Treatments 3 617046 205682 1.09 5.42 Birds/trts 144 27163426 188634 Appendix Table 16. Analysis of variance of body weight gain of male turkeys at 16 weeks of age. Source of Degree of Sum of Mean F variation freedom square quare Statistic f.001 Treatments 3 964901 321633 .668 5.42 Birds/trts 144 69231928 480777 Appendix Table 17. 54 Analysis of variance of body weight gain of female turkeys at 4 weeks of age. ource of Degree of Sum of Mean F ariation freedom square square Statistic f.001 Treatments 3 28554 9518 2.01 5.42 .Birds/trtsl 113 553127 '4729 'Appendix Table 18. Analysis of variance of body weight gain of female turkeys at 8 weeks of age. Lource of Degree of Sum of Mean' F variation freedom square Isquare Statistic f.001 Treatments 3 304756 101585 1.47 5.42 Birds/trts 118 8142144 69001 55 Analysis of variance of body weight gain of female turkeys at 12 weeks of age. Appendix Table 19. Source of Degree of Sum of Mean F variation freedom square square Statistic f.001 Treatments 3 658928 219642 1.07 5.42 Birds/trts 118 24109923 204321 Appendix Table 20. Analysis of variance of body weight gain of female turkeys at 16 weeks of age. Eource of Degree of Sum of Mean F ariation freedom square square Statistic f.001 Treatments 3 202864 67621 .214 5.42 Birds/trts 118 37250276 315680 Appendix Table 21. 56 Analysis of variance of taste panel scores for intensity of flavor of light meat of male turkeys slaughtered at 16 weeks of age. Source of Degree of Sum of Mean F variation freedom square square Statistic f.05 Treatments 3 4.16 1.386 .713 2.2 Expt. lérrors 44 85.51 1.943 Appendix Table 22. Analysis of variance of taste panel scores for intensity of flavor of dark meat of male turkeys slaughtered at 16 weeks of age. errors 122.75 Source of Degree of Sum of Mean F variation freedom square square Statistic f.05 Treatments 3 1.723 .574 .205 2.2 Eth° 44 2.789 57 Appendix Table 23. Analysis of variance of taste panel scores for intensity of flavor of light meat of female turkeys slaughtered at 16 weeks of age. Source of Degree of Sum of Mean F variation freedom square square Statistic f.05 Treatments 2 1.5 .75 .358 2.2 xpt . rrors 33 69.25 2.09 Appendix Table 24. Analysis of variance of taste panel scores for intensity of flavor of dark meat of female turkeys slaughtered at 16 weeks of age. Source of Degree of Sum of Mean F variation freedom square square Statistic f.05 Treatments 2 2.06 1.03 .317 2.2 xpt. Frrors 33 106.92 3.24 Appendix Table 25. 58 Analysis of variance of taste panel scores for acceptance of flavor for light meat flavor of male turkeys slaughtered at 16 weeks of age. Source of Degree of Sum of Mean F variation freedom square square Statistic f.05 Treatments 3 1.73 .576 .291 2.2 Eth' 44 87.09 1.979 Frror Appendix Table 26. Analysis of variance of taste panel scores for acceptance of flavor for dark meat flavor of male turkeys slaughtered at 16 weeks of age. Source of Degree of Sum of Mean F variation freedom square square Statistic f.05 Treatments 3 2.99 .997 .435 2.2 th° 44 100.68 2.288 rror Appendix Table 27. 59 Analysis of variance of taste panel scores for light meat flavor of female turkeys slaughtered at 16 weeks of age. xpt. rror Source of Degree of Sum of Meal F variation freedom square square Statistic f 0.05 Treatments 2 2.05 1.02 .463 2.2 33 72.84 2.20 Appendix Table 28. Analysis of variance of taste panel scores for dark meat flavor of female turkeys slaughtered at 16 weeks of age. gource of Degree of Sum of Mean F variation freedom square square Statistic f 0.05 Treatments 2 2.66 1.33 .441 2.2 33 99.34 3.01 xpt. rror Appendix Table 29. 60 Analysis of variance of taste panel general acceptance scores (juiciness, tenderness) for light meat of male turkeys slaughtered at 16 weeks of age. ource of Degree of Sum of Mean F ariation freedom square square Statistic f.05 Treatments 3 7.823 2.607 1.09 2.2 Fth' 44 105.17 2.39 rror Appendix Table 30. Analysis of variance of taste panel general acceptance scores (juiciness, tenderness) for dark meat of male turkeys slaughtered at 16 weeks of age. gource of Degree of Sum of Mean F variation freedom square square Statistic f 0.05 Treatments 3 1.723 .574 .272 2.2 44 2.108 xpt. rror 92.757 Appendix Table 31. 61 Analysis of variance of taste panel general acceptance scores (juiciness, tenderness) for light meat of female turkeys slaughtered at 16 weeks of age. Source of Degree of Sum of Mean F Nariation freedom square square Statistic f 0.05 Treatments 2 .66 .221 .15 2.2 “Pt“ 33 68 09 2 06 error ' ‘ Appendix Table 32. Analysis of variance of taste panel general acceptance scores (juiciness, tenderness) for dark meat of female turkeys slaughtered at 16 weeks of age 0 error Source of Degree of Sum of Mean F variation freedom square square Statistic f 0.05 Treatments 2 3.39 1.695 .709 2.2 Eth' 33 78.92 2.39 Appendix Table 33. 62 Analysis of variance of the feed efficiency of turkeys at 4,weeks of age. Source of Degree of Sum of Mean F variation freedom square square Statistic f.05 Treatments 3 0.02 0.006 0.8 24.3 Expt. lerror 4 0.03 0.0075 Appendix Table 34. Analysis of variance of the feed efficiency of turkeys at 8 weeks of age. xpt. rror Source of Degree of Sum of Mean F variation freedom square square Statistic f.005 Treatments 3 0.03 0.01 2.00 24.3 4 0.02 0.005 63 Analysis of variance of feed efficiency Appendix Table 35. of turkeys at 12 weeks of age. Eource of Degree of Sum of Mean F ariation freedom square square Statistic f.05 Treatments 3 0.08 0.026 1.06 24.3 FXP‘“ 4 0.1 0.025 rror Appendix Table 36. Analysis of variance of feed efficiency of turkeys at 16 weeks of age. ' gource of Degree of Sum of Mean F variation freedom square square Statistic f.05 Treatments 3 .36 .12 1.09 24.3 Expt. Frror 4 .44 .11 Appendix Table 37. 64 Analysis of variance of mortality from the 1 day to 16 weeks of age for all the experimental groups of the turkeys uesed in the experiment. Source of Degree of Sum of Mean F variation freedom square square Statistic f.05 Treatments 3 7.38 2.46 .56 24.3 Expt. rror 4 17.5 4.37 MICHIGAN STATE UNIVERSITY LIBRARIES 3 1293 03043 6186