I‘ E‘ d ’f .V .d D ‘ I V M I - ‘I. ‘ ".2 .~ . ar— ' - - v.4. . . -aa ""~L_ . i I.) - 3‘. I «511:4 - ." o \ I": .‘.‘v,‘.(- --- O- . ”'73:” 90. ~r:::';. ' o A I o. :1: t"; T‘z‘: - . .u.’ ' 1“."‘3'. - gm :ux'v'Mv—HN ."3 This is to certify that the thesis entitled Effects of Various 1m Formulations on the Control of Insects Cannon to Potatoes and Beam presented by Arthur Ham Blower has been accepted towards fulfillment of the requirements for 11.8. degree in Bataaoloy jor professor Date July—1.0! 3953 . A ‘. ' f . _~ , . fL'Qul" y‘.“ .".‘ ‘ J In. ‘. V k ‘ ' . ‘ \ 7“! - v...- . . ""x‘ V I l . 5! «mafia. IVV- . l , - ‘!.‘ :-~ . 'i-:l“n"‘.’ "'4 ‘ ‘1. .'.7§£Ihl.slv‘}, ’3' -' V .a' - . . ' 0’ I. . f I ' '. v ' o‘.b'~l"..5. - I“ r" . . l. s \ ' . . '7 ' ‘~' _ . - . c . 'A'. 3.6. .Iz'.. . - ...‘~.V.1:.’| .Il:‘-"- 9 ‘Iss , . . . Idibflwl-Jmlmepn '. H - o 4 -"‘ ‘u. . A — . .‘ V3?“ 1 L UV] Sub“ 1 EFFECTS OF VARIOUS DDT FORMULATIONS ON THE CONTROL OF INSECTS COMMON TO POTATOES AND BEANS By Arthur Warren Bloomer AN ABSTRACT Submitted to the School of Graduate Studies of.Michigan State College of Agriculture and Applied Science in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Department of Entomology Year 1953 Approvefi¢ 1 7m 7632,? 3(955 Arthur Warren Bloomer Studies were made during the growing season of 1952 to determine the effects of various formulations of DDT in the control of insects common to potatoes and beans. Five dif- ferent wettable powders were tested in these studies. Three of the formulations were 75 percent DDT wettable powders pre- pared and furnished by the Michigan Chemical Corporation. Another formulation was E. I. du Pont de Nemours and Com- pany's 75 percent DDT wettable powder sold under the trade name of Deenate. The fifth formulation was Sherwin-Williams Company's 50 percent DDT wettable powder. All Sprays were applied at the rate of one pound of actual DDT per hundred gallons of water. ‘ Sprays were applied to the potato plots with a tractor drawn sprayer which received its power from the "power take- off" mechanism of the tractor. Two hand-drawn, gasoline powered Sprayers were used to apply sprays on the bean plots. Sweeping counts were made at various periods after Spraying to determine the amount of‘insect control obtained. Yield determinations were made on the potato plots, but none were made on the bean plots. The populations of insects were generally smaller on the-beans and larger on the potatoes. This was probably due to the great differences in the rates of application of the spray materials. The potatoes received an average of'llS‘ gallons of spray material per acre whereas the beans re- 308885 1 Arthur Warren Bloomer ceived from 225 to 250 gallons of spray material per acre. Drift had a greater effect on the potatoes than it did on the beans. No apparent difference was great enough in the control obtained by any of the formulations used to allow the recom- mendation of any one of them over the rest collectively, or any one singly. EFFECTS OF VARIOUS DDT FORMULATIONS ON THE CONTROL OF INSECTS COMMON T0 POTATOES AND BEANS By ARTHUR WARREN BLOOMER A THESIS Submitted to the School of Graduate Studies of Michigan State College of Agriculture and Applied Science in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE xi Department of Entomology \- 1953 2;} ~\‘\ \ \\ THESIS ACKNOWLEDGMENTS The writer wishes to express his deepest appreciation to Professor Ray Hutson, Head of the Department of Entomology, for his guidance in the conduct of this work; to Dr. Herman L. King for his assistance and guidance in the preparation of this manuscript; to Dr. J. R. Hoffman for his advice and interest. in this work; to his wife, Barbara, for the typing of this manu- script; to Oscar Taboada for his suggestions in the preparation of the manuscript; and to the graduate students in the Entomology Department for the assistance and c00peration they gave through- out the course of this work. Finally the writer wishes to ex- press his sincere appreciation to the Michigan Chemical Corp- oration for the support of the field work under which this work was done and for the insecticial materials which they supplied. II. III. IV. V. VI. CONTENTS Introduction . . . . Review of Literature A. Potatoes . . . . B. Beans . . .'. . Procedure . . . . . A. Potatoes . . . . B. Beans . . . . . Experimental Data . Discussion . . . . . Conclusions and Summary Literature Cited . . O Page cc (n -q b) \p 11 1h 64 70 72 INTRODUCTION Insects have long been pests of man. They have damaged his health, his home, his animals and his food. Much work has been done 'to find control measures for injurious insects, and much more will 'be done in the future. Perhaps one of the most important types of insect damage is the harm they cause to field crOps. Modern methods of agriculture produce conditions favorable for insects. The resulting infestations <2ause inestimable damage to farm products every year. At present the largest field of research for insect control is ‘with insecticides. The advent of DDT and the numerous other synthetic insecticides which followed have stimulated unpredictable interest in this field. Industry as well as governmental agencies spend much Inoney every year on research. The advent of these modern insecticides has helped agriculture as well as many other branches of human livelihood. Recommendations .for the use of insecticides usually refer to the insects involved. 'Various factors other than insects must also be taken into considera- ‘tion when making these recommendations. Two of the more important ones are the formulations used and the host plants to be treated. 'This paper deals with the control of insects common to both potatoes wand.beans with DDT. Five formulations of DDT wettable powders were used to see if any differences in control resulted from the dif- tferences in formulations. 'zriblt L Tamar: I . Fit”. 61.4 a W a ..i Iv. REVIEW OF LITERATURE POTATOES: Prior to DDT there was no one insecticide which would control the majority of potato insects. Muncie and Morofsky (20) have written a report of their findings which partially summarizes potato insect control before the advent of DDT. ‘With the discovery of DDT and the large amount of pmmli- city that accompanied it, much experimental work was carried out to see what insects it was effective against. 'Granovsky (8), reported in l9hh that DDT gave very good control of the potato flea beetle, Egitrix cucnumeris (Harr.), and showed promising results in the control of the Colorado potato beetle, Leptinotarsa decemlineata, (Say); tarnished plant bug, Lygug lineolaris P. deB.); and other mirids. Swingle and Mayer (25) reported that DDT as a Spray at the rate of one pound per hundred gallons of water, caused complete mortality of all instars of Colorado potato beetle infesting potato plants in an outdoor garden. Weigel (31) stated that in laboratory tests with potato cuttings DDT gave complete kill of Colorado potato beetles and potato flea beetles. Roark and McIndoo (22 & 23) stated that DDT was very effective against thevpotato flea beetle and the Colorado potato beetle. They also said that it gave good control of the six-Spotted leaf- hOpper, Macrosteles divisus (Uhl.) and the potato leafhopper, Empoasca fabae (Harr.). L 1hr, 3' ‘ —-q. an“ . 1.. In 1945 workers added more data to that of previous years. Thompson (26) attributed large increases in potato yeild to the control of the potato leafhopper by DDT. Fox and Perron (7) reported that DDT gave the best control of potato leafhopper nymphs, while the arsenicals the poorest control of nymphs. zThe plots protected by DDT also gave them the best yields. Gyrisko, Jodka, and Rawlins (9) found that DDT produced very favorable reductions in infestations of Colorado potato beetle, aphids, flea beetles, and leafhoppers. They further reported that insect populations remained at low levels for ten or more days after application. In their work, dusts did not have the residual power that sprays did, and they noticed no phytotoxi- city. Bruce and Tauber (3) stated that DDT not only controlled the potato leafhopper and flea beetle, but also controlled the potato aphid, Macrosiphun solanifolii (Ashmead) as well. In 19h6 Gyrisko, Wene, and Rawlins (10) reported that DDT ‘ gave better control of the potato aphid and the green peach aphid,.flygp§ persicae (Sulzer) than such standard aphicides as rotenone, nicotine, and thiocyanates. They found that only nicotine fumigation gave better control than DDT. These workers reported that at least two pounds of DDT, as a Spray or dust, was necessary for good control. Bronson and Smith (2) found that DDT applied as an emulsion, aerosol, dust mixture, or suspension was effective in reducing aphid populations. They found that the dust was more effective, but more DDT per acre was applied with the dust. {)4 .rzms 5. Morofsky and Muncie (IO), and Muncie and Morofsky (19) re- ported in 19h? that DDT applied either as a spray or dust gave the best control of potato insects. Kulash (13) reported the same year that DDT gave good control of Colorado potato beetle larvae. He also found that chlordane and benzene hexachloride gave good control of the larvae but that benzene hexachloride did not have the residual power of DDT. Kulash (It) also found that DDT gave good control of the potato flea beetle and the tobacco flea beetle, Epitrix hirtipennis (Mels.). The DDT dusts proved to have more residual power than did the Sprays of DDT and benzene hexachloride. Heuberger and Stearns (12) investigated the apparent growth- promoting effects of DDT and reported their findings in l9h8. They decided that the better growth might result from leafhopper control. In their work they found that DDT gave excellent con- trol of leafhoppers and high yield reaponses, DDT had no fungicidal value, and DDT caused no apparent injury to potatoes. Voss and Andre (30) found that DDT oil emulsion gave better control of aphids than DDT wettable powder. (Campbell and Pepper (A) re- ported in 1948 that parathion was outstanding in aphid control and resulted in the highest yield, whereas plots Sprayed with DDT and benzene hexachloride were similar in yield and these yields were not significantly lower than that produced by the use of parathion. They also found the DDT oil emulsion gave better aphid control than DDT wettable powder. Turner and Woodruff (28) found that DDT provided better flea beetle control Lianne "- .l. “'2' I on potatoes. than chlordane, methoxychlor, and toxaphene. They also stated that DDT spray powder of fine particle size provided significantly better control of flea beetles and higher yields than did DDT spray powder of coarse particle size. Morofsky and Muncie (17) found that DDT gave better potato leafhopper control, in general, than did benzene hexachloride although DDT varied greatly in its effects upon the six-Spotted leafhopper. Doyle and Duncan (6) in comparing the effects of DDT with calcium arsenate, chlor- dane, and Lethane "B-72" found that DDT was the most effective insecticide in~ controlling potato insects and that it significantly increased potato yields. Tuner and Woodruff (29) reported in 1949 that yields from fields Sprayed with DDT wettable powder were significantly higher than yields from fields sprayed with DDT emulsion even though the DDT emulsion gave better control of aphids. The DDT wet- table powders gave better flea beetle control. Mitchene (15) reported in 1950 that when potatoes were heavily infested with both potato leafhoppers and Colorado po- tato beetles, DDT gave the best control and yields as compared With aldrin, dieldrin, toxaphene, and calcium arsenate. Morof— Sky and Muncie (18) reported that while DDT emulsion and wettable powder gaVe good control of potato flea beetles and leafhoppers, an emu1310n of 25% DDT and 3% Parathion gave better aphid control than either of the preceding. Many other workers have investigated the control of potato insects, the above being a small representation. It is felt » Mir); ,‘—" .. I...- .~\ufi«i fl ~... “ca—.5. 7. that most of the findings and views are shown by the sources cited. BEANS: Various workers have tested DDT on beans with nearly the same results. Weigel reported in l9hh that DDT caused no mortality to the Mexican bean beetle, Epilachna varivestis (Mule) adults or larvae when applied as a Spray or dust on plants in a green- house. Nelson (21) and Roark and McIndoo (22 & 23) also report- ed that DDT was not very effective in controlling the Mexican bean beetle. Harries (11) reported that bean leaves dusted with DDT killed 36 percent of the third instar Mexican bean beetle larvae that fed on them. Bjornseth (1) reported in 19h6 that DDT, if properly applied, gave adequate and economical control of leafhoppers on beans and substantially increased the yield. Turner (27) stated that DDT was much less effective than methoxychlor or rotenone in Mexican bean beetle control. In l9h8 Ditman and Cory (5) reported that DDT as an aerosol was ineffective in controlling the Mexican bean beetle. Not much work has been done with DDT on beans because of its inability to control the major pest, the Mexican bean beetle. -N-‘ 1'4". PROCEDURE POTATOES: On May 26, 1952, twelve hundred pounds of certified Sebago seed potatoes were planted on approximately two-thirds of an acre of muck located at the Michigan State College Experimental Muck Farm in Clinton County, Michigan. They were planted in forty- eight rows, two hundred feet long, the rows being three feet apart. The rows ran in a northerly and southerly direction. On the north and west sides the potato field was bounded by a sugar-beet field. The sugar beets were later plowed under because of the extreme weedi- ness of the field.) The south side of the field was bounded by a willow hedgerow and the east side by a weal-filled ditch. A gravel road was located east of the ditch. Six plots were established with two four-row replicates in each plot. The plots ran from east to west in a consecutive order (see Fig. 1). One plot was designated as the check plot and was sprayed with fungicide only. The other plots were Sprayed with a fungicide and one of the insecticides. The insecticides used consisted of five formulations of wettable powder DDT. Three of the formulations were 75 percent DDT wettable powders furnished by the Michigan Chemical Corporation of St. Louis, Michigan. One formulation was E. I. du Pont de Nemours and Company's 75 percent DDT wettable powder sold under the trade name of Deenate. The other formulation was Sherwin-Williams Company's (abbreviated in Figures and Tables as Sher-Will) 50 percent DDT wettable powder. All of these formulations were applied at the rate of one pound of DDT per hundred gallons of water. I *th. Tribasic Copper Sulfate was used as the fungicide for these toms and was applied to all of the plots. The rate of application for the fungicide was three pounds per hundred gallons of water. Approximately 110 gallons of Spray material were applied per acre. All plots were sprayed on the same day with two hours elapsing fun the time of starting to the time of finishing. The sprayer used in these tests was a tractor-drawn John Bean sprayer with mnmle tanks (see Fig. 3). Each tank could be emptied separately mulwas capable of holding one hundred gallons. Both tanks were tmed as speed up the process of Spraying. Enough of the chemicals were Tufighed in advance to make twenty-five gallons of spray material for each plot. Material for two consecutive plots was mixed in the sprayer simultaneously. The hoses and boom were thoroughly flushed out to eliminate errors due to any other chemicals which might have been present in the system. The Sprayer tanks were always thoroughly flushed before any materials were added. By taking these pre- cautions it was felt that errors were kept at a minimum. The sprayer received its power through the "power take-off" mechanism of the tractor. A four-row potato boom was used with three nozzles per row. Number three spray discs were used in the nozzles, and these discs were changed after every three days of Spraying. This was done to prevent too great a variance in the rate of aruflication. The material was arplied at 225 pounds of pressure. 7’1:th "N L !~. Hf: E 10. All plots were sprayed five times with the applications being nude on the following dates: June 19 & 30. July 7, 21 & 28. TM)strips in each replicate were left unsprayed on July 7. This was accomplished by laying a 15' x 20' tarpaulin on the potato plants mnirunning the sprayer over it. The tarpaulin shielded the plants from U19 spray and a fifteen-foot Skip Spot was left. The purpose cfi‘this added variation was to see if the insects would build up on the skipped areas. Sweepings were made with a standard insect sweeping net. They were taken immediately before spraying and three days after Spraying. By not Spraying on July 1h sweepings were also obtained for ten, fourteen, seventeen and twenty-one days after Spraying. Only the middle two rows of each replicate were used in making the sweeping counts. This was done to remove the influence of spray drift from the other plots. The sweeping proCedure used was to make five complete oscilla- tions through the center two rows of each replicate with a standard Size sweeping net. Each oscillation was accompanied by a step forward thus making;one complete oscillation per step. The sweepings were taken from randomized areas in the replicates each time. Sweepings were taken on the following dates: June 25 & 30. July 3, 7, 10*, 14*, 17*, 21*, 2h, 28 & 31. August 7, ll, & l3. * Sweepings also taken in strips protected from spray.on July 7. L 7.1 C_)~”‘.g.; -..,.—fi “as. a...) 11. The insects collected by sweeping were killed in cyanide jars and placed in labelled paper bags. The insects were identified and counted in the laboratory. I Yield data were taken on September 19, 1952. Two forty-foot strips were dug in each replicate, one strip in each of‘the center two rows of the replicate. All of the U. S. No. l and No. 2 grade potatoes from these strips were gathered and weighed. The weights of each grade were recorded separately. This gave a total of four 'yield samples for each plot. BEANS: On June 10, 1952, Red Kidney beans were planted on a part of the Michigan State College farm located in Ingham County, Michigan. They were planted in five replicates of twenty-five rows each. The rows were twenty feet long and two feet apart. The replicates were separated by eight-foot aisles of'bare soil. Later, two replicates ‘were disced under because of their extreme weediness. The rows ran in an easterly and westerly direction (see Fig. 2). The area was bounded on the north side by five rows of beans. The 'west side was bounded by a field of ensilage corn, and the south and east sides by the dirt service road. Six plets were established in this field with three replicates per plot. Each spray plot replicate Contained four rows each, and the check plot replicates contained five rows each. The Spray ‘materials for the beans were the same as those used on the potatoes 'with one exception; no fungicide was used on the beans. The DDT was applied at the rate of one pound of actual DDT per hundred gallons of‘water, the same as the potatoes. The rate of application per acre was between 225 and 250 gallons. C‘CLBJS V ;|- I" ah; 4%" sA-I 5) vh r:- (n a" 12. All of the plots were sprayed on the same day with two hours elapsing from the time of starting to the time of finishing. Two different Sprayers were used in Spray applications. The first three sprays were applied with a Lowell Cyclone wheelbarrow sprayer (see Fig. A). It had a capacity of fifteen gallons and Sprayed with fifty pounds of pressure. The rest of'the Sprays were applied with a John Bean Spartan sprayer (see Fig 5). It also had a fifteen-gallon capacity, but it sprayed at a pressure of 200 pounds. Both Sprayers 'were hand-drawn with motors supplying power for Spraying. The Spray guns were hand operated. Sprays were applied on the following dates: August 1, 8, 15, 22 & 29. September 5 8c 12. Sweeping counts were taken immediately before and three days after spraying. The sweeps were taken lengthwise of the rows sampled. To eliminate as much as possible the effects of drift, only the xniddle two rows of the Spray replicates and any two of the middle three rows of the check replicates were sampled. Four sweeps per row or eight per replicate were taken at each Sweeping. Sweeping counts were made on the following dates: July 17*. August 1*, A, 8, 12, 15, 18, 22, 25 & 29. September 3, 5, 8 & 12. * Counts made preliminary to any spraying. The insects collected in the Sweeping counts were killed in cyanide bottles and placed in paper bags. The bags were labelled and the insects were later classified and counted in the laboratory. 13. been had ods the p 3 ans a on the be taken ere les w samp ield No y Sis. analy chemical d for picks "L151 . r. ‘ul. .1" EXPERIMENTAL DATA 4:53}: 11+. ’3:AT"~13:£I‘:‘.‘.‘27'I‘ OF TORITO 1 A .Le ”- "I3. is L 1 Check » Wan): --_. 'l'31‘Vs . revive-1.. 15. Check Deenate SherAWill 1335 l l I I I l l I I I l I I I I 4 Deenate I sum: 1 L ...il.... l o. .3. -.|s,\.H lid! 30" *z. 16. FIG. 3. JOHN BEAN SPRAYER USED FOR SPRAYING POTATOES. FIG. 1.. LOWELL WHEELBARROW SPRAYER USED FOR SPRAYING BEANS. FIG. 5. JOHN BEAN SPARTAN SPRAYER USED FOR SPRAYING BEANS. 7 AI rug-5‘5 Average Number Of Insects Per Sweep. 10* \It FIG. 6. NUMBER OF POTATO LEAFHOPPERS ON POTATOES AT 17° VARIOUS DAYS AFTER SPRAYING. LEGEND : / / / 1333—- - - -— / , 133h—-- —- I 1335—----—-— Sher-Will----——- Deenate ’0 \ / Check 7’ \ 7 10 Number 0! Days After Spraying. . . mI-I Total Number Of Insects For All Sweeps. 18. FIG. 70 NUMBER OF SIX-SPOTTED LEAFHOPPERS ON POTATOES AT VARIOUS DAYS 2 I 5 AFTER SPRAYING. 20 \\ / \ H \J'I l L I I ax/ \ \ \ \ \ \ \\ \ \ / I ‘ I l LEGEND: \_ // 5 I ‘\ I 1333 ----- , . ss__ \ / ,’ 1335-——---- ———— ‘ Sher—Will-——---——— // Deenate 4 Check 1_ 3 7 10 1h 17 Number 0! Days After Spraying. Pf‘_h‘s Total Nvmber 0f Insects For All SI-reeps. 10* / . \ 1333 ————— / 133a-— - - —- 19. FIG. 8. NUMBER OF O'I'Hm LEAFHOPPERS ON POTATOES AT V.-"-.RI(_)US DAYS AFTER SPRAYING. \ LEGEND: , \ LBS—“"— \ Sher-Will — \ Deenate I Cheek Number 01‘ Days After Spraying. 't‘s‘fil.‘ Average Number Of Insects Per Sweep. FIG. 9. NUMBER OF SPITTLE-BUGS ON POTATOES AT VARIOUS DAYS AFTER SPRAYING. LBEEND: / I ‘ 133 ---- 2.0 i 1332—— - - —— 1335 — - - - —- I Sher-Will —— - -—--— Deenate 4 Check 1.5“ l J l J Number Of Days After Spraying. 20. to“; Average Number Of Insects Per Sweep. 21. FIG. 10. NUMBER OF TARNISHED PLANT BUGS ON POTATOES 3T VARIOUS DAYS AFTER SPRAYING. 2.0 T L 1.0“ I/ -I I ,’ LEGEND: 1 ,,// 1333-- - - - ,,’ 133h— - - —- I 1335—-- - — Sher-wm—- -——— ,5 Deenate Check 3 7 10 1A 17 21 Number Of Days After Spraying. !r_:s($ 2:151}; v ramifig .uh Average Number Of Insects Per Sueepo Jr [JILILLLI W N IJILI l I nlJmll _J 21+. FIG. 13° NUMBJR OF PLEA BJSTLBS ON POTATOSS AT VARIOUS DAYS AFTER SPRAYING. LEGEND: / // I 1333----- / 133a —- —— / 1335-— --- — ' Sher-Will—— -— I I Deenate ‘// Check Number 0! Days After Spraying. -515 I .5: 1. n.. III/i II I 5|.I 0mm a am e. 32. an e 3 0:3. 33% Signage 52% * iii-.. 'lll'lv'llul £838 803 @3980 wcaaoozm 3qu ’lll 'al’ IDIIII‘C-II x0020 ”wx , 3233 (. ll .. [Hafiionm .00 ’un n'mMMH . ll null¢mma . l Illnmmma .05 52mg 10m 6m . H 09. an." t. .Nmmd qmmgm mo Qonmm Emma. gunman mmoeinbm 20 mmMLAOEfinA oefiSm mo mmmzaz 12.. .cHh 'edeens 9AM .103 810981.11 3'0 .19qu eBuuv 3:151: “a .1. 1.. Total Number Of Insects Fbr Sweeps On All Plotso 26. FIG. 15. NUMBER OF SIX SPOTTED LEAFHOPPERS ON POTA'roas DURING '1‘st PERIOD OF summit, 1952., * 30* 20‘ 10‘ iiéiouli'zziz'hzsaid'z‘lil} June July Aug. Dates Sweeping Samples Were Takeno * Spray application dates: June 19 k 30; July 7, 21 & 28. flu... ‘J .1. ~ IN 27. FIG. 16. NUMBER OF APHIDS ON PO‘I‘IRTOES DURING TBST PERIOD OF SUI/373R, 1752. 3" 360 350 l 310 I 300 290 1333--- — _ / I I l: LEGEND: / 280 1331. — " —— 1335 — -- - — . 250 Sher-Will—- -— I . Deenate I, a 21.0 Check I I e we N H O x g 4‘ ‘ when...“ \\‘ 190* I H 3 \ \— 170« I “160. 150* i/,//flc;/ O! 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RS / a. . / a 1m "ozmaq 1. 1&3 .mmasm me 822 .52 SEE 8848a zo mcDm p.25; BurmHzWJwH 25E. dams mono 923m m0 552:2 .mH .UHm °sdeens mm .103 unseen]; go .19qu eSeJeav sny,‘ 30. W 9326 a /0#803 “H O ledziozm J n \ l I - - IRS m1 s \ \ I}... l <\\ I. I 5 -133 ow m. a ” \\\ 5584 m. J _ . u u \\ mN Nu w t a m a. m u \ m a . I .s _ a \ . on . .\ mm m .mmaa . ,. n n mo 82$ .59 0233 weeps“. 20 92.8%..- .2. .92 32. NUNBSR OF POTATO LEAFHOPPERS ON BEANS FIG. 21. 2 h‘ AT VARIOUS DAYS AFTER SPRAYING. 2.01 5 ft: 1.; m ‘- 3 U) 1 O 5 .. in 0 CL . m *6 . 0 U) l: H q M O h jg 1.0‘ 0 q no «3 t; - 5 <1: / Number Of Days After Spraying. IIII .1 ~ .ll EM Total Number Of Insects For All Sweeps. 33. FIG. 22. “UVBflR CF SIX-SPOTTiD LiAFHOPPSRS ON BEANS AT VARIOUS DAYS AFPQR SFRAYINQ. 16' LEGEND: 15‘ 1333“——-— 1331.—---— 133s——-- —— ‘ Sher-Willr------ Deenate Check Number Of Days After Spraying. Minis Total Number Of Insects For All Sweeps. 701 65* 60‘ 55‘ 50‘ 20‘ 15 ‘ 10+ 3l+e FIG. 23. UUIBSR OF ”FREE LJAFEUPFURS ON BEANS AT VARIOUS DAYS AFTER SPRAYING. LEDEND: 1333'" " ‘ " 13315— ' ' — 1335‘— “ ‘ ‘ ‘— Sher-Will—-— - —— Deenate Check Number Of Days After Spraying. .- r1515 ,1}.INA Total Number Of Insects For All Sweeps. 35. no. 21.. “I’D-'33? OF SUITTL; 2:11:13. :31: mans AT VARIOUS DAYS AFTER SPRAYING. l3 ‘\ 10 . LSJEND: 1333 ----- . 133a—-- — 1335——-———— Sher~Will ' Deenate Check I \ /'/”f7 “)6/ /* ” Number Of Days After Spraying. M. < - “n—A‘.‘ -_——.u-.x- In my ‘ .1—1‘ ' . Total Number Of Insects For All Sweeps. 181 15‘ H O 1 \h L 1 L 36. FIG. 25. NUMBER OF TARNISHED PLANT BUGS ON BSANS AT VARIOUS DAYS AFTER SPRAYING. IDOL-3ND: 1333 ----- l33h— - - —— 1335 —‘ ‘ ‘ ‘— Shen—h’ill—w - —— .‘Oeenate CbeCk / Number of Days After Spraying. .‘ .0! J” ~r LV- _ J .I‘.‘h.‘ 'v. “I” ‘lt"tn~'44‘.o...: Wrihl Total Number Of Insects For All Sweeps. 37. FIG. 26. Nva‘xBER OF OPHER PLANT BUGS ON BEANS 551 AT VARIOUS DAYS AFTER SPRAYING. 1 50‘ 1.5‘ -- F l': r. i 1.0. 1f 1.4234111): I 1333- — -- - g . 133h—‘ ‘ _ .I 35 1335 — -- — "‘ Sf:er—'.r.'ill———- - -———- Deenate Check 30‘ 254 20* 15 ~ ,, ll / / I // I 10 1 ,’ - /-:_____,_.—-—-—""- f, _.—v—‘—-';-7‘— // I / / w”, ’_ .. / I”; 51’ ,..../ ("I 3 7 Number Of Days After Spraying. ~- ' 15‘; Total Number Of Insects For All Sweeps. 38. FIG. 27. NOB-(BER OF APHIDS ON BEANS AT VARIOUS DAYS AFTER SPRAYING. 20‘F\\\ ‘.—"- _,__———--" 19"}‘(f . \ 18 ~‘\\\‘ //’ 17‘ ‘\\\\\\\\ 1a / / \ 15" / ”/” 11‘. ” I’ ll, 13. ’,,/’/ ’/ ll-‘ 10* 12 M, / / 1333 ----- /// 1” l33h‘—“"__‘ /// 1335-——-----—- SherQWill‘——““—_‘ ///.,’ ' Deenate ’ Check Number Of Days After Spraying. ‘94: . e . Total Number Of Insects For All Swesns. 111 101 FIG. 28. NUMBER OF FLEA BEETLES ON BEANS AT VARIOUS DAYS AFTER SPRAYING. LEGEND: 1333 ----- 133a—--— 133s—--- —— Sherewill - Deenate Check Number Of Days After Spraying. .NH m . hobfimpumm mom fl mm .md am as warms: unmade COHuwofiaan hauam * [0-0 e .ceme one; moaaasm mafiommzm mopma Sousa obscene II - IISSLEm II -- Fiend .l - - [in I - - .. ARA "Q23umq / \AV\\\ \ fimm ., C“ \\ T: g \ 1m 4 °sdbens eats Jog sqoeeul Jo Jeqmmu eSeaeav I \ a 41~4 s .1. . .) ..1 o - . 5......5... . .5 SN. .5. 3.. a 3.: .5 93.2 .1: . . .4 3%.. N. to 72.. . e .. A: . .. m .. at? 5.58.. so $8.51 .3 SE 41. TABLE I PHYSICAL DATA OF FORMULATIONS TESTED Obtained from Michigan Chemical Corporation, L.E. Pauling, representive. 9-23-52. Sherwin- 1333 1331a 1335 Deenate Williams f _‘__7 % DDT 75.1 75.3 75.8 76.2 50.1+ Average mean partic e Diameter (microns) 2.3 2.32 2.1+]. 3.33 1.99 -200 mesh (dry) 100% 99.98% 99.98% 99.66% 100% Suspension2 1.56% 0.35% 2.18% 0.059% 1.36% pH3 7.20 7.30 7.21. 7.92 7.98 Wettability“ - Floc. - Floc. - 1 As determined by Fisher Sub-Sieve Sizer (Air Permea- tion Method). 2 Figures show the actual % DDT left in suspension 30 minutes after the formation of a 2.5% DDT by weight suspension. 3 Reaction of a 1.76 suspension (58 of the powder sus- pended in 120 gr. of distilled water). 1* Appearance of scum or flocculation in a 1.76 suspen- sion with tap water. Days After Spraying 10 lb 17 21 10 1h 17 21 10 1h 17 21 Total Number of Sweepg 1.0 50 no 30 20 10 A 40 5O 40 30 20 10 #0 5O #0 3C) 20 1C) TABLE II POTATO INSECTS Average Number Of Insects Per Sweep PLOTS 1535 Deenate 1:525 Sher- Check 12;; Will 6.3 9.2 7.7 13.6 8.6 1A.6 .1. .3 .3 .8 .3 .5 .3 0 kl #4 1‘ CD Potato Leafhogpers .6 1.6 1.9 5.A 3.3 10.2 .2 .2 .h .6 .3 .h .6 .5 .6 .9 1.0 1.2 1.2 1.6 1.3 2.5 . 2.5 2.7 1.9 1.8 1.9 '5.8 h.2 h.5 Six4Spotted Leafhoppers .2 .l .2 .A .3 .2 . .2 .1 .3 .h .5 .8 .h .3 .5 .7 .5 .5 chgglleafhoppers ”o o o .2 .2 .2 .1 .1 o O O O 0 .1 .1 .1 0 .1 F4 0* C) C) i“ C) .A .9 1.7 5.6 1.8 6.h .h .2 .4 .8 .5 O O c: :a ta so no <3 7115183111??? '. I 2 g ! quk lg...- A. i Days After Spraying 10 lb 17 21 10 1A 17 21 10 1h 17 21 TABLE II CONTINUED Total Number of Sweeps DO 50 A0 30 20 10 #0 50 DO 30 20 10 #0 5O 40 30 20 10 43. Average Number Of Insects Per Sweep Check 1332 Will 1.0 1.1 .8 .7 1.0 .9 nan Sher- 1221-: Deenate 1335 Spittle Bugs .6 .6 .6 .8 .6 1.0 1.1 1.3 1.6 1.2 1.1 1.5 1.1 1.3 1.2 1.6 1.1 i 1.5 1.5 1.5 1.0 1.2 .9 .8 1.2 2.1 2.3 1.1 2.0 2.2 Tarnished Plant Bugs .6 1.0 .5 .h .5 1.2 1.2 1.1 1.6 1.5 1.6 1.6 1.2 l.h 1.9 1.A 1.1 .9 1.1 1.2 l.h 1.3 2.1 .9 1.3 1.9 1.5 1.2 l.h 1.6 Other Plant Bugs 0 0 .1 -.l .2 .2 .2 .1 .9- .2 .3 .A .2 .3 .2 .5 .3 .5 .h .7 .h .A .3 .3 .6 .3 .2 .8 .5 1.0 -2, 1'... n.- _ " 'A ”Ft! {9— ul— rt- mgr-5.0.1 L. 1 Days After 10 lb 17 21 10 1h 17 21 Total Number of Spraying Sweeps no 50 '40 30 20 1o 40 50 40 30 20 10 TABLE II CONTINUED #6. Average Number Of Insects Per Sweep 2212251222 1.0 1.8 1.0 2.3 2.0 5.7 2.9 8.6 15.4 29.5 .h .8 .3 0 1.2 .2 .8 .h 1.5 2.1 1.9 1.1 2.5 4.5 ma Sher- y_1__1_1_ 122]: Deenate M 2.0 1.6 1.9 2.3 1.8 2.h 8.0 9.7 5.5 11.6 17.3 12.3 2h.3 30.7 35.9 1.0 1.2 1.5 Flea Beetles 0 0 O .1 .1 .1 .1 .2 .2 1.6 1.5 1.8 1.6 1.1 2.2 3.5 h.7 5.6 1212 1.7 2.2 6.3 10.8 20.6 1.h .1 .2 .1 3.7 1.6 9.6 f 45. TABLE III NUMBER OF POTATO LEAFHOPPERS 0N POTATOES Average Number Of Insects For Five Sweeps 21.0.12 2199 9153315 1133- Sh'er-Will 1334 Deenate 1322 June 25 ------------------ No Record --------------- '--- June 30 18 16 11 13 9 22 July 3 84 9 11 10 10 3 July 7 75 16 10 12 15 16 July 10 21 2 1 15 1 2 July 14 74 11 8 a 6 5 July 17 97 16 9 16 14 7 July 21 133 51. 23 23 28 60 July 24 19 2 1 1 2 1.. July 28 67 12 4 7 8 5 July 31 3 0 0 0 0 1 Aug. 4 15 3 2 1 l 2 Aug. 7 20 2 1 l 0 3 tug. 11 15 2 1 3 2 1, 1g. 13 20 4 1 3 1 2 gyalJl‘m gap qu225 qua30 JulyB Jub'7 .hdy'lo July 16 July 17 July 21 July-24 hfly28 July 31 Aug. 4 4us. 7 2g.411 9. JL?. TABLE IV NUMBER OF SIX SPOTTED LEAFHOPPERS 0N POTATOES Average Number of Insects For Five Sweeps Check 1322 Sher-Will PLOTS Deenate 46. 1335 ------------------- No Record------------—----- 0 2 1 2 2 3 z. 2 5 2 1 1 o o QHOHHHwWF-NONHN 1m 0 0 1 1 1 0 1 1 4 5 2 0 2 2 2 o 6 2 1 0 1 o 1 1 0 o 0 0 HHQNHNwMNle-JHN F9 <3 P’ c: x» A) 26 p— as x» u: 26 h' A) "'1“- .FW. . I". L c»: Qppg June 25 June 30 July 3 July 7 July’ll) July 14 July.17 4JU1}’.21 uQLLy 2%; July 28 TABLE V NUMBER OF LEAFHOPPERS OTHER THAN POTATO AND SIX-S POTTED LEAFHOPPERS 0N POTATOES Average Number of Insects For Five Sweeps Check 6 O 0 0 1 0 C) 1 C) .1 C) 1. C) (J C) PLOTS .1223. HOOOI—‘OOOOOOOOI—‘N SherAWill 1334 6 OOOOOOOI—‘OOOOOH l. l-‘OOOOOOOOi-‘OOON 473 Deenate 1335 5 3 0 O 0 0 0 O O 0 1 2 0 0 0 0 0 0 l 1 0 1 0 0 0 0 0 0 1 0 ‘75???" - ~.r'~v Is I 1+8. TABLE VI NUMBER OF APHIDS 0N POTATOES Average Number of Insects For Five Sweeps 3.142.152 { 9.2.13.2 911.29}; 1333 Sher-Will 133p Deenate 1335 3 June 25 l 0 2 2 2 1 i June 30 1 3 o 2 2 3 2 July 3 5 5 6 1 2 5 : July 7 4 12 8 5 ll 7 July 10 4 16 12 15 10 9 July 14 8 21 6 4 11 3 July 17 7 6 7 8 5 July 21 6 7 9 6 6 July 24 6 10 5 3 8 4 July 28 5 8 9 10 10 7 July 31 6 6 18 13 18 15 Aug. 4 8 18 32 25 27 39 Aug. 7 29 92 149 181 96 114 Aug. 11 35 117 159 247 176 151 Aug. 13 148 286 ' 191 304 354 204 TABLE VII NUMBER OF TARNISHED PLANT BUGS 0N POTATOES Average Number of Insects For Five Sweeps 2.1.9.12 933:9 Q1395 1223 Sher-Will 1__3_3_4 Deenate June 25 O 2 3 l 3 June 30 11 14 13 11 14 July 3 10 4 8 7 5 July 7 5 17 11 15 22 July 10 6 7 10 2 4 July 14 6 9 12 8 12 July 17 7 12 12 10 8 July 21 5 8 7 6 7 July 24 4 1 2 2 2 July 28 6 4 4 5 2 July 31 1 1 l o 1 Aug. 4 2 1 2 o 2 Aug, 7 5 l 1 1 3 Aug. 11 3 2 2 2 1 Aug. 13 6 3 3 0 2 49. 1335 2 l4 5 21 3 13 H \J'I NOUOOWNOO. 2523 Junejfl) July 3 July 7 July 10 July 14 July 17 July 21 July 24 July 28 July 31 Aug. 4 Aug. 7 Aug. 11 Aug. 13 TABLE VIII NUMBER OF PLANT BUGS OTHER THAN TARNISHED PLANT BUGS 0N POTATOES Check 1 1 5 1 3 7 4 1 2 0 2 l l l PLOTS 12.3.}. N OOOD—‘Ol—‘OV'IWI—‘OO-‘O Sher-Will b.) C) C) *4 <3 C) A) CD to \n 10 C> A) +4 1334 1 <3 k4 c> <3 c: 14 +4 \w v: +4 #4 1a c> Average Number of Insects For Five Sweeps 50. Deenate 1335 0 OHOOONHWWWOWH HOHHOHWO‘NWHNOH 'u 1.3- mum-1““. .l‘n-n l m H A TABLE IX NUMBER OF FLEA BEETLES ON POTATOES Average Number of Insects For Five Sweeps 21212 92.1.7.2 M 1333 Sher-Will % Deenate 1222 July 3 . l 0 0 0 0 0 July 7 C) 0 0 0 0 0 July 10 0 0 0 0 0 0 July 14 1 0 0 0 0 0 July 17 7 l 3 3 3 2 July 21 14 3O 24 21 25 53 July 24 3 1 0 0 0 1 July 28 18 4 3 3 4 5 July 31 2 o 0 o o 0 Aug. 4 13 0 0 0 0 1 Aug. 7 10 2 0 1 0 0 Aug. 11 0 0 1 0 0 Aug. 13 2 O 0 1 1 TABLE X NUMBER OF SPITTLE BUGS 0N POTATOES Average Number of Insects For Five Sweeps 52. 119.18. Date 933933 1333 Sher-Will 1334 Deenate 1335 June 25 3 0 0 1 3 2 June 30 4 8 15 8 14 8 July 3 5 7 7 6 12 8 July 7 8 12 18 22 21 17 July 10 7 2 4 3 2 July 14 12 9 9 6 9 4 July 17 7 9 12 13 10 14 July 21 3 7 6 14 13 10 Ju1y 24 6 3 2 3 1 2 Ju1y28 3 4 4 4 6 ' 6 July 31 2 1 o o o 1 Aug. 4 2 2 1 2 l 2 Aug. 7 3 2 1 o 2 0 Aug. ll 2 4 o 2 1 4 Aug. 13 2 1 4 1 2 1 53. TABLE XI POTATO YIELD IN BUSHELS PER ACRE Based On One Bushel Equals Sixth Pounds 821.3. Replicate Check 1333 Sher-Will 1334 Deenate 1335 Grade - U.S. No. 1 l 146 354 384 436 498 318 2 383 281 475 482 342 535 3 465 628 352 442 451 421 _4_.. .223. 2.9.4 .526 23.1. 4.112 2.52 Average 389 467 442 473 435 408 Grade - U.S. N0. 2 1 33.2 33.2 30.2 25.7 24.2 36.3 2 .36.3 30.2 39.3 30.3 36.2 43.9 3 36.3 33.2 43.9 33.2 30.3 24.2 ._4_ 42.4 13.6 16.2. 2;ng 161.1 $3.33 Average 37.0 27.6 37.4 29.1 31.8 29.5 Days After Spraying Total’ Number of Sweegs 144 144 144 144 144 144 144 144 144 144 144 144 TABLE XII BEAN INSECTS Average Number Of Insects Per Sweep Check 133;_ Will 1.8 2.4 .4 .3 PLOTS Sher- Potato Leafhoppers 1334 Deenate .1 .1 .1 .1 .3 .4 .7 .3 Six-Spotted Leafh0ppers O O .1 O O .1 O O Other Leafhoppers 0 O O O .3 .2 .3 .2 fipittle Bugs 0 O O O O O O O Tarnished Plant Bugs 0 O O O .1 .1 O O Other Plant Bugs 0 .1 O O .1 .1 0 .1 51+. # 55. TABLE x11 CONTINUED Average Number Of Insects Per SweeE Days Total _ PLOTS. After Number of Sher- Sgraxing Sweegs Check 1333 Will 1334 Deenate 1335 Aghids 3 144 .1 .l .1 .1 O O 7 144 .l .1 .l .l .1 .l Flea Beetles 144 .l O O O O O 7 _ 144 O 0 0 O 0 O A Mexican Bean Beetles . 3 . 144 O O O O 0 O 7 144 O O O 0 0 O l— a— —....- is pl—uvwmm‘xulih‘os .1 O ‘ ‘ ~ b . u '4 TABLE XIII NUMBER OF POTATO LEAFHOPPERS 0N BEANS Average Number of Insects For Five Sweeps 93.2 2193.4 July 17 23 Aug. 1 44 Aug. 4 22 Aug. 8 45 Aug. 12 19 Aug. l5 17 Aug. 18 8 Aug. 22 5 Aug. 25 4 Aug. 29 3 Sept. 3 2 Sept. 5 1 Sept. 8 o SePt. 12 2 ELQI§ 12;; 23 13 54 42 1 1 5 4 1 1 4 6 o o 1 '2 1 o 1 o o o 0 o o 0 o o Sher-Will 1334 21 24 1 8 I-‘N H ONOOHOOH Deenate 15 p O OOOOOOOOVINPO 56. 1335 17 .p p OOHOOOHOWH'WO TABLE XIV NUMBER OF SIX SPOTTED LEAFHOPPERS ON BEANS Date July Aug. Aug. 17 Aug. 8 Aug. Aug. Aug. Aug. Aug, Aug. Sept. 12 15 18 22 25 29 3 Sept. 5 Sept. Sept. 12 Average Number of‘Insects For Five Sweeps Check 13 OOOOO'OOOOOwl-Jw 1323 Sher-Will 1334 1 00000000000045“ PLOTS 2 OOOOOOOOOD—‘NON ll OOOOOOOI—‘Ot—‘OOW Deenate 1332 3 3 3 5 l O 1 l O l 1 O O O 0 0 O O O O O O O O O 0 O O 57. Date July Aug. Aug. TABLE XV NUMBER OF LEAFHOPPERS OTHER THAN POTATO AND SIX-SPOTTED LEAFHOPPERS ON BEANS Average Number of Insects For Five Sweeps 17 Aug. 8 Aug. Aug. Aug. Aug. Aug. Aug. Sept. 12 15 18 22 29 3 Sept. 5 Sept. 8 Sept. 12 Check w NONPQ-PNI—‘HHl-‘OP PLOTS 123; Sher-Will 1334 1.4 OOOOO‘l-‘HOl-‘OOOO l OOOOmI—‘OOOOOOO Deenate 1335 OOOOQP—‘i—‘OHOHOHN l HOOOW'OOOOOOOP 1 OOOOOHHOHOOOH '{w pus-Abr- l. NUMBER OF APHIDS ON BEANS TABLE XVI Average Number of Insects For Five Sweeps Date July Aug. Aug. Aug. Aug. Aug. Aug. Aug. Aug. Aug. 17 1 4 8 12 15 18 22 25 29 Sept. 3 Sept. Sept. Sept. 12 Check 2 OOOOOOHI—‘OOOOW PLOTS 1333 Sher—Will 1334 O OOOOHOHl—‘OOHHH O OOOONOI—‘NOOONH O c> <3 <3 c> zu c> <3 P‘ <3 c> he .a .. Deenate 1335 O O 2 O O O O O O O 1 O l O 1 ' O O l 1 2 O O O O 0 0 O O 59. “in. .3“;'\ I“; .A 4 .‘vj 11 .v a: '0‘: a. TABLE XVII NUMBER OF TARNISHED PLANT BUGS ON BEANS Average Number of Insects For Five Sweeps Date July 17 Aug. Aug. Aug. 8 Aug. Aug. Aug. Aug. Aug. Aug. Sept. Sept. Sept. Sept. 12 15 18 22 25 29 3 12 Check 4 HOOOOOl—‘l-‘l—‘Ol-‘NO OOOOOOOOOOi—‘OH 1333 Sher-Will 1334 2 PLOTS 1 OOOOF—‘OOl—‘OOHOH C> c> c> c5 C> C) C) C) re <3 <3 0 P' l Deenate OOOOOOOOOOOONP 60. 1.; b) \1) U1 OOOOOOOHOOOOOHI 221:3 hflyl? Aug. 1 Aug. 4 Aug. 8 Aug. 12 Aug. 15 Aug. 18 Aug. 22 Aug. 25 Aug. 29 Sept. 3 Sept. 5 Sept. 8 Sept. 12 NUMBER OF OTHER PLANT BUGS ON BEANS TABLE XVIII Average Number of Insects For Five Sweeps Check 8 6 2 2 5 1 2 1 2 3 l 1 l 2 PLOTS 1333 Sher4Will 1334 7 HOOONOOOOOOON 3 H O HOOOOOOI—‘OOHO 3 CDCDCDCDIM’F-‘(DOOCDCDOJ> Deenate Us.) OOOOHOOOOOOO? 61. 1335 OOOOOOOOOOHOJ-‘Vw ‘ 3“ ., ... =4.-—4.‘1 at: M}. be: Nu' Innu- ‘ l. I NUMBER OF FLEA BEETLES ON BEANS TABLE XIX Average Number of Insects For Five Sweeps 2353 July 17 Aug. 1 Aug. 4 Aug. Aug. 12 Aug. 15 Aug. 18 Aug. 22 Aug. 25 Aug. 29 Sept. 3 Sept. 5 Sept. 8 Sept. 12 Check 1 OOOOOOOHOHOOO 1333 Sher-Will 3334 O OOOOOOOOOOOOO PLOTS O OOOOOOOOOOOOH O OOC>C)l-"’OCDCDCDOCDCD!"-J Deenate 1335 O l O 0 O O O O O 0 0 0 O O O O O 0 O O O O O O O O O 0 62. NUMBER OF SPITTLE BUGS UN BEANS TABLE XX Average Number of Insects For Five Sweeps Aug. 12 Aug. 15 Aug. 18 Aug. 25 Aug. 29 Sept. 3' Sept. 5 Sept. 8 Sept. 12 Check 3 OOOOHOHOOOHNN 1333 Sher-Will 1334 1 OOOOOOOOOOOOH PLOTS l OOOOOOHOOOOOH l OOOOOOOOOOOOH Deenate $332 1 1 O 1 O O O 0 O 0 O O O 0 0 l O O O O O O O O O O 0 0 63. lieu—Mm. on. ~‘ WWMHAIQ'KSI k DISCUSSION The five formulations of DDT that were used in this work were of three types, generally speaking. Michigan Chemical Corporation‘s 1334 and E. I. du Pont de Nemours and Company's Deenate were of the flocculating type; Michigan Chemical Corpora- tion's 1333 and Sherwin Williams Company's formulation were of the intermediate suspension type; and Michigan Chemical Corpora- tion's 1335 was of the high suspension type. 'd m my-m‘w.—..m__——1_ I! ‘ I Sankeur (24), in his report in 1952, stated that Michigan Chemical Corporation's 1334 and E. I. du Pont de Nemours and Com- pany's Deenate were similar in amounts initially deposited and retained but were inferior to the other three preparations. He further stated that Michigan Chemical Corporation's 1335 resem- bled in behavior the SherwinAWilliams Company's product but that the former product was generally superior to all of the others in amounts initially retained. He flaund that Michigan Chemical Corporation's 1333 and SherwinAWilliams Company's product were superior in lasting qualities with the former being superior to all others in lasting qualities and inferior to all others in amounts initially deposited. He also found from the analysis of samples from unsprayed plots that drift was an important factor. This study was primarily concerned with three specific insects and members of five families of insects that were found 64 65. on both potatoes and beans. These were the potato leafhopper, the six-spotted leafhopper, the tarnished plant bug, other members of the families Cicadellidae and Miridae, members of the family Aphididae, adults of the family Cercopidae, and flea beetle adults of the family Chrysomelidae. POTATOES: I Very definite and good control was obtained for the potato I leafhopper. In all cases pOpulations of this insect on the ! sprayed plots remained below the pOpulations on the check plot. 1 Control of this insect varied little with the formulation used 1 up to ten days after spraying (see Fig. 6). After ten days, variations appeared, but the data was insufficient to justify any comparisons. Drift apparently affected the pOpulations of this insect on the check plot (see Fig. 14). A The populations of the six-spotted leathpper showed that this insect was rather evenly dispersed throughout the field re- gardless of spraying (see Fig. 7). Spraying did affect the Populations to varying degrees throughout the testing period (see Fig. 15). Based on the populations on the check plot DDT gave poor control of this insect (see Table IV). Drift may have obscured the true results in this case. DDT gave some control of the other leathppers found on potatoes. The difference in populations on the Sprayed plots and the check plot were slight (see Fig. 8). In most instances the spittle bug pepulations were greater 66. on the sprayed plots than.the populations on the check plot (see Fig. 9). This can be SXplained most clearly by the habits of the adult Spittle bugs. These insects move about and are easily disturbed. The disturbance caused by spraying may have caused many to move to the weeds bordering the field or the spray may have killed those that were on the potatoes at the time of spray- ing. In either case the insects would move into the field again after a period of time had elapsed and settle on the more succu- lent foliage of the sprayed plants (see Fig. 20). The tarnished plant bug pOpulations behaved in much the same way as did those of the spittle bugs (see Fig. 10). These in- ‘sects are strong fliers and readily disturbed and may have flown to the weeds during spraying operations and returned later. These insects require meristematic tissue to oviposit in and would naturally collect on the more succulent foliage of the Sprayed plants. Spraying either controlled the insects present at the time of spraying or caused them to leave the field and remain for a short time (see Fig. 17). The other plant bugs found on potatoes appeared to be con- trolled to some extent by DDT (see Fig. 11). These insects may have behaved in nearly the same manner as the preceeding two, but in most instances populations on the Sprayed plots were low- er than those on the check plot (see Fig. 18). Aphids appeared to benefit from Spraying with DDT. The data show clearly that aphid populations were generally higher on the Sprayed plots than on the check plot (see Figs. 12 & 16). —<—.._.__ 67. Aphid populations continued to increase on the sprayed plots at a much faster rate throughout the testing period than they did on the cheek plot. These facts verified previous findings that DDT wettable powders give little or no control.of aphids. Ap- parently, at the rate applied, DDT kills the parasites and pre- dators that prey on the aphids but not enough aphids to lower the reproductive potential markedly. The flea beetles on potatoes were definitely controlled by DDT. Good control was obtained up to ten days after spraying (see Fig. 13). POpulations then increased rapidly if no DDT was applied. Drift appeared to influence the numbers of these insects on the check plot to a large extent (see Fig. 19). The various formulations did not seen to vary too much in the amount of control they gave. In all instances the sprayed plots gave higher average yields of U. S. No. 1 potatoes than did the check plot. The plot receiving Michigan Chemical Cor- poration's 1335 did not give an average yield much higher then the check plot, but both of these plots had one replicate which bordered on weedy margins of the field. These two plots re- ceived a greater amount of insect damage because the migration of insects from the weeds into the field would have come pri- marily through the replicates which bordered the weeds. BEANS: At the rate applied, DDT gave excellent control of potato leafhoppers on beans (see Fig. 21). Drift may have influenced !-“~ .q! 1‘14. "-4.7 nun-r.- . 1.x“-.gaz 68. the populations of this insect on the check plot, but not to the extent that it did on the potatoes (see Fig. 29). The populations of the six-Spotted leafhopper were small throughout the testing season. After spraying started, this insects' numbers were reduced to practically nothing and re- mained low during the rest of the testing period (see Table XIV). Apparently the heavy rate of application was the chief reason for such complete control. DDT gave good control of the other leafhoppers found on the beans. In most cases the sprayed plots had populations below those of the check plot (see Table XV). There was a marked increase in these insects on August 29 although only seven days had elapsed since the last spray application (see Table IV). Good control was obtained up to seven days after Spraying (see Fig. 23). Good control of epittle bugs was obtained. The insect populations remained low for at least seven days after Spray- ing (see Fig. 24). Migration into the plots after Spraying did not seem to be a factor. Fair control of the tarnished plant bug was obtained also. The numbers of insects on the Sprayed plots remain- ed below the numbers of this insect on the check plot al- though they built up faster than the spittle bugs did (see Fig. 25). Migration onto the plots was probably a larger factor in this case. DDT gave very good control of the other plant bugs on ‘. M“ ' '- 69. beans. P0pulations on Sprayed plots remained low for at least seven days after spraying (see Fig. 26). After Spraying had started, pOpulations on the check plot were always higher than populations on the sprayed plots although in most cases the difference was small (see Table XVIII). The aphid population was of low density throughout the test period (see Table XVI). Only winged forms were observed or collected. DDT had little or no apparent effect on the aphids unless it was to attract them to the sprayed plots be- cause of the white powder which remained as a residue from spraying (see Fig. 27). The populations of flea beetles were low on all plots. DDT apparently gave some control but the numbers of insects were quite low and it would be difficult to state the effects exactly (see Fig. 28). CONCLUSIONS AND SUMMARY 1. The pOpulations of insects were in general smaller on the beans and larger on the potatoes. This was most likely due to the great differences in the rates of application of the Spray materials. 2. Drift had a greater effect on the potatoes than it did on the beans. 3. No apparent difference was great enough in the control ob— tained by any of the formulations used to allow the recommenda- tion of any one of them over the rest collectively or any one singly. A. Good control was obtained for the potato leathpper on both the potatoes and beans. 5. Little if any control seemed to be obtained for the six- spotted leathpper on potatoes while good control was obtained .for this insect on beans. 6. Good control of other leathppers was obtained on both potatoes and beans . '7. Spittle bugcontrol was questionable on the potatoes, xmigration being a possible factor. On beans, good control of spittle bugs was obtained. 23. Control of the tarnished plant bug followed the same pat- tern as the control of spittle bugs, being questionable on potatoes and good on beans. 9. Only fair control was obtained for other plant bugs on gustatoes whereas good control was obtained for them on beans. 70 71. 10. Aphids appeared to benefit by the spraying of DDT on potatoes. Aphid pOpulations were higher on the Sprayed plots than on the check plot. The pOpulations of this insect were insufficient on the bean plots to allow a definite statement. 11. Definite and good control of flea beetles resulted from the application of DDT on potatoes. Not enough flea beetles m. d fi'l V.” 7 were present on beans to warrant a definite statement, but DDT apparently gave some control of this insect on these I -—':‘4l‘.‘..lvuw ‘- Lad. plants. 4. 5. 7. 11), LITERATURE CITED Bjornseth, E. H. 1946 One Year's Results From Dusting Snap Beans to Control Anthracnose and Leafhoppers. Michigan Ag. Expt. Sta. Quart. Bul., 28:3, 191-193. 1 Bronson, T. E. and Smith, Floyd F. 1946 Control of Aphids on Potatoes in Northeastern Maine. Jour. Econ. Ent., 39:189-194. Bruce, Willis N. and Tauber, Oscar E. F 1945 Trials With DDT on Potatoes, Cabbage, and Squash. 1 figur. Econ. Ent., 38:439-441. 4 Cam bell John C. and Pepper, Bailey B. l 48 Promising New Chemicals For the Control of Diseases and Insects. Amer. Potato Jour., 25:82-6. Ditman, L. C. and Cory, E. N. 1948 Liquefied Gas Aerosols to Control Bean Beetles. Jour. Econ. Ent., 41:268-275. Doyle J. A. and Duncan, J. 1948 Comparison of Calcium Arsenate, Chlordane, Lethane, and DDT in the Control of Potato Insects. Seventy- ninth Annual Report of the Entomological Society of Ontario. pps. 22-24. Fox, C. J. and Perron, J. P. 1945 Report on a Potato Leafhopper Control Experiment at Ottawa with DDT and Copper Sprays. Seventy- sixth Annual Report of the Entomological Society of Ontario. pps. 27-31. Granovsky, A. A. 1944 Tests of DDT for the Control of Potato Insects. 2222-.EQQQ- Eat., 37:493-499. Gyrisko, George G.; Jorka, Joseph F. T. and Rawlins, W. A. 1945 DgTigg Eggtrol Potato Insects. £232. Eggn. §Q§., 3 : "' o Gyrisko, G. G.; Wene, George P. and Rawlins, W. A. 1946 DDT to Control Potato Aphids. Jour. Econ. Ent., 39:205-208. 72 11. 12. 13. 14. 15. 16. 17. 18. 195 ZCL. 21,- 73. Harries, F. H. 1944 Laboratory Tests With DDT Against the Pea Aphid and the Mexican Bean Beetle. Jour. Econ. Ent., 37:151. Heuberger, J. W. and Stearns, L. A. 1948 Compatibility of DDT and Fungicides on Potatoes. Jour. Econ. Ent., 39:267-268. Kulash, Walter M. 1947 Benzene Hexachloride, DDT, and Chlordane for Colorado Potato Beetle Control. Jour. Econ. £32., [+0 :640-6143 0 Kulash, Walter M. 1947 DDT and Benzene Hexachloride for Potato Flea Beetle Control. Jour. Econ. Ent., 40:651-654. uwu-v..--—.....__.—__._ _ __ . . , ' .V ‘.».l ..'- - .' h- .=.‘-.‘! i Mitchener, A. V. E 1950 A Comparison of Recent Insecticides with Calcium g_ Arsenate for Control of Potato Insects. Jour. Econ. Enio ’ [6'3 :176-1780 Morofsky,'W. F. and Muncie, J. H. 1947 The Use of New Insecticides in the Control of Potato Insects. Amer. Potato Jour., 24:5, 162-166. Morofsky, W. F. and Muncie, J. H. 1948 Use Of Certain New Materials in the Control of Potato Insects in Michigan. Amer. Potato Jour., 25:6, 255-259. Morofsky, W. F. and Muncie, J. H. 1950 Effectiveness of Certain Insecticidal and Fungicidal Sprays in Control of Leafhoppers, Aphids, Flea Beetles, and Early Blight on Potatoes. Michigan Ag. Expt. Sta. Quart. Bul., 32:3, 307-310. Muncie, J. H. and Morofsky, W. P. 1947 Results of Spraying and Dusting Potatoes in Michigan in 1946. Amer. Potato Jour., 24:6, 183-187. Muncie, J. H. and Morofsky, W. F. 1947 Results of Spraying and Dusting Trials With Fungi- cides and Insecticides on Potatoes, 1938-45. Michigan State College Agr. Expt. Sta. Tech. Bul. 204. Nelson, R. H. 1944 Field Experiments on DDT for Control of the Mexican Bean Beetle. Jour. Econ. Ent., 37:151. 22. 23. 24. 25. 26. 27. 28. 29. L30. 131. 74. Roark, R. C. and McIndoo, N. E. A Digest of the Literature on DDT Through April 30, 1944. U. S. D. A. Bureau of Ent. and Plant Quarantine. December, 1944. E-631. Roark, R. C. and McIndoo, N. E. A Second Digest of the Literature on DDT. (May 1, 1944, to December 31, 1944) May, 1946. E-687. Sankuer, Raymond F. 1952 Report on a Study of DDT Residues on Treated Plants During the 1952 Growing Season. Unpublished re- ports, Fall, 1952. j Swingle, M. C. and Mayer, E. L. J 1944 Laboratory Tests of DDT Against Various Insect Pests. k Jour. Econ. Ent., 37:141-142. 3 Thompson, R. W. *"' 1945 DDT for Potato Leafhopper Control. Seventy-sixth annual Report of the Ent. Society of‘Ontario. pps. 22- 26. Turner, Neely. 1946 Organic Insecticides for Control of the.Mexican Bean Beetle. Conn. Agr. Expt. Sta. Bul. 512. ppS Q 68-69 9 Turner, Neely, and Woodruff, Nancy. 1948 Chlorinated Insecticides for Control oftshe Potato Flea Beetle. Jour. Econ. Ent., 21:328-329. Turner, Neely and Woodruff, Nancy. 1949 Effect of Formulations of DDT and of Chlordane on Potato Flea Beetle and Yield. Jour. Econ. Ent., #23270'h730 Voss, C. M. and Andre, Floyd. 1948 Observations of Aphids on Potatoes in Northern Wisconsin, 1947. Amer. Potato Jour., 25: 266-272. Wiegel, C. A. 1944 DDT Against Some Pests of Vegetable CrOps. Jour. Econ. Ent., 37:150. / :l JI/r’; ’0 f \ \ . \ \. k..— L-~~ 3.3- . 114.1 ti‘» ROOM USE 05 I IIIHIIIIIIHHIUIIH MVIWI‘JI 03058 III I N' l ' I I' l l l l l ' lllllllllllllll 2054 93 31