THE EFFECT OF ORAL Am INJECTABLfi {RON-
ON THE HEMATOLQGECAL VALUES Am
GROWTI-i WWW GF MSTEIN CALVES

Thesis {or the Degree of M. S.
MEXICAN STATE UNEVERSETY
Samuei M. Getty

1964

THESIS

LIBRARY

Michigan State
University

 

 

ABSTRACT
THE EFFECT OF ORAL AND INJECTABLE IRON ON

THE HEMATOLOGICAL VALUES AND GROWTH
WEIGHTS OF HOLSTEIN CALVES

by Samuel M. Getty

Mammals, shortly after birth, exhibit physiologically
low hemoglobin values. Numerous theories have been proposed
to explain this phenomenon. Because mammalian milk is be-
lieved to be a perfect food by many, one exception to this
belief stands out in the literature. Milk is known to be
sufficiently deficient in iron to produce an iron deficiency
with relative ease. Because of the vast amount of material
on this subject in the literature only selected references
were used in a review of the literature. Clinically, a wide
range of hemoglobin values are observed from calves less
than twenty weeks of age. A study was undertaken to deter-
mine if any effect in body weight or hematology is notice-
able when iron is Supplied to calves either orally or intra-
muscularly.

Forty-five holstein calves were obtained when less
than 7 days of age. Initial hemoglobin values were arranged
in a linear fashion and a group consisted of fifteen calves
selected from every third value. One group of calves re-
ceived 30 mg. of iron orally each day while another group

of 15 calves received two injections of iron (1,000 mg.

Samuel M. Getty

total) at 7 and 21 days of age. The remaining calves served
as a control group.

Calves were weighed and blood samples taken weekly.
Body weight gains were not significant; however, the pattern
of growth varied. Weight gains were compared to hemoglobin
levels. A correlation was observed between the portion of
calves receiving oral iron that had a higher mean level of
hemoglobin and the increased rate of daily gain. Calves
which received intramuscular iron and had low hemoglobin
values showed a better rate of daily gain than calves with
high hemoglobin values.

Hemoglobin and packed cell volume values showed a
significant increase at approximately 7 and 21 days respec-
tively after the calves were started on iron. A reticulo-
cytic response was observed in calves treated with iron.
Calves treated with oral iron showed a gradual but uniform
response while calves treated with injectable iron showed
a peak response for the three weeks during treatment and
then returned to the normal range.

Serum iron levels were determined weekly by the di-
pyridal method. A significant increase (P <1.01) was ob-
served in all calves receiving injectable iron.

It is concluded from this study that iron either
in intramuscular or oral form is beneficial in meeting the
clinical requirement of the dairy calf as a supplement to

whole milk. Although a great response is not seen in bovine

Samuel M. Getty

hemoglobin values, as compared to the response in baby pigs,
it was found that the physiological anemia was prevented

in calves with the use of supplemental iron.

THE EFFECT OF ORAL AND INJECTABLE IRON ON
THE HEMATOLOGICAL VALUES AND GROWTH

WEIGHTS OF HOLSTEIN CALVES

BY

Samuel M..Getty

A THESIS

Submitted to
Michigan State University
in partial fulfillment of the requirements
for the degree of

MASTER OF SCIENCE

Department of Surgery and Medicine

1964

ACKNOWLEDGMENT

VI wish to extend my appreciation and gratitude to
the following peOple who contributed their time and efforts
in this study.

To Dr. Gabel H. Conner, Professor, Graduate Advisor,
Department of Surgery and Medicine, for his cooperation and
advice in this study and his patience in the writing of this
thesis.

To Dr. David J. Ellis, Director of Ambulatory Clin-
ics, Department of Surgery and Medicine, for his cooperation
and stimulation during this time.

To Dr. Clifford C. Beck, Extension Veterinarian,
Department of Surgery and Medicine, for his valuable sug-
gestions and help in the research.

To Dr. Leonard D. Brown, Associate Professor, De-
partment of Dairy, who provided the initial suggestion which
encouraged these studies. Also, my sincere gratefulness
for the arrangements which were made prior to this study.

To Mr. Dennis Armstrong, Manager, Dairy Herd Oper—
ations, and the Department of Dairy for providing the hous-
ing, feed, and care of these animals.

To Dr. Elwyn R. Miller, Associate Professor, Depart-
ment of Animal Husbandry, for his critical evaluation.

To Dr. Wade Brinker, Chairman, Department of Surgery

and Medicine, for his cooperation during the study.

ii

Dedicated to

Lucy and the girls

iii

TABLE OF CONTENTS

CHAPTER PAGE

I O INTRODUC TION O O O O O O O O O O O O O O . O O O
II. REVIEW OF THE LITERATURE . . o o o . . o o o 0
III. MATERIALS AND METHODS. . . . . . . . . . . . .

Experimental Animals 0 o o o o o o o o o

\O\O\ONl-‘

Experimental Procedures. . . . . . . . .

IV. RESULTS AND DISCUSSION . . . . . . . . . . . . 12

V. CONCLUSIONS. . . . . . . . . . o . . . . . o . 26
APPENDIX . . . . . . . . . . . . . . . . . . . . . . . 27

BIBLIOGRAPI'IY O O O O O O O O O O O O O O O O O O O O O s 1

iv

FIGURE

1.

2.

LIST OF FIGURES

PAGE

Steps in Heme Biosynthesis (Taken from

Rimington).....o............. 6

Reticulocyte Response from Iron Treatment, for

the Three Groups of Calves. . . . . . . . . . . 23

TABLE

1.
2.

3.

4.

5.
6.

7.
8.

9.

TABLE

1.
2.
3.

LIST OF TABLES

Mean Body Weights of Calves, by Group. . . . . .
Average Daily Gain (1b.) at Weekly Intervals, by
Group. . . . . . . . . . . . . . . . . . . . . .
Weight Gain in Comparison to Initial Hemoglobin
Level. . . . . . . . . . . . . . . . . . . . . .
Mean Hemoglobin Values for the Three Groups of
Calves . . . . . . . . . . . . . . . . . . . . .
Average Packed Cell Volume by Calf Group . . . .
Mean Erythrocyte Values for Control and Iron
Treated Calves . . . . . . . . . . . . . . . . .
Calculated Blood Indices by Calf Group . . . . .
Average Reticulocyte Values for the Three Groups
of Calves. . . . . . . . . . . . . . . . . . . .

Mean Serum Iron Values by Calf Group . . . . . .

LIST OF TABLES FOR HEMATOLOGICAL
FINDINGS AND BODY WEIGHTS AS
FOUND IN THE APPENDIX

Ca1fNo.l...................
calfNOQZOOO0000000000000...

Ca1fNo.3...................

vi

PAGE

13

14

15

17
19

20
21

22
24

PAGE

28
28
29

TABLE

4.

5.

6.

7.

8.

9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.

Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf

No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.

NO.

\0 (D \I 03 U" IF
0

10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.

vii

PAGE

29
30
30
31
31
32
32
33
33
34
34
35
35
36
36
37
37
38
38
39
39
40
40
41

TABLE

28.
29.
3o.
31.
32.
33.
34.
35.
36.
37.
38.
39.
4o.
41.
42.
43.
44.
45.

Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf
Calf

Calf

No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.
No.

N00

28.
29.
30.
31.
32.
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.

viii

PAGE

41
42
42
43
43
44
44
45
45
46
46
47
47
48
48
49
49
50

CHAPTER I

INTRODUCTION

The medicinal value of iron has been realized for
some time. The element was a valuable tool in treating
blood disorders, battle wounds, chlorosis, and other con-
ditions during the 17th century and probably before that
time.

More recently iron studies have been numerous in
regards to absorption, excretion, metabolism, and require-
ments for the various animal species. Radioactive tracer
techniques have been employed with much success in studying
the role of this element.

A great deal of attention has been focused on the
physiological low hemoglobin values of immature animals.
The role of iron in relation to hemoglobin formation and
body weight is unsettled at this time. Confusion is para-
mount, as evidenced by the wide range of "normal" hemoglobin
values published for calves. A desire to determine the ef-
fect of iron administered parenterally and orally on the
formation of hemoglobin and on growth of calves stimulated

the following study.

CHAPTER II
REVIEW or THE LITERATURE

The Egyptians and Greeks were probably the first
to use iron for medicinal purposes although its function
was not known at that time. Fowler (1936) reported that
during the 17th century two English physicians, Sydenham
and Willis, used the element to treat chlorosis. Later,
Lemary, Geoffry and Menghinis found iron to be a necessary
constituent of the blood.

Vannotti and Delachaux (1949) believe iron is pres-
ent in all tissues and is an essential element to the ani-
mal. Tint and Reiss (1950), and Bernhart and Skeggs (1943)
reported that the element is present in the hemin-chromo-
proteins and, as such, Coryell and Pauling (1940) are of
the opinion that it is directly responsible for oxygenation
and oxidation at the cellular level. Hahn et a1. (1943)
reported that siderophilin and ferritin are two non hemin—
chromoproteins which contain iron. Schade and Caroline
(1946) believe that siderophilin or transferrin is that
protein fraction of the plasma serving as an iron carrier
in the plasma iron transport system. Finch and Finch (1955)
reported thatstorage iron is found intracellularly as a pro-
tein complex ferritin; while Bessis and Breton-Corius (1959)

have demonstrated hemosiderin to be a condensation or

clustering of ferritin molecules. Hahn (1937) reported that
blood hemoglobin constitutes the greatest quantity of iron

in the body. Later Hahn (1948) reported that iron is pres-
ent in the cytochrome system but this mechanism is not af-
fected by an iron deficiency. Schultze (1941) reported that
the cytochrome oxidase activity of bone is normal or elevated
in a state of iron deficiency.

Cartwright (1956), Ullrey (1958), Matrone (1954),
and Smith (1944a, 1944b) have reported that a typical iron
deficiency produces a hypochromic-microcytic anemia in swine,
calves, rats, and rabbits. Follis (1958), Wohl and Goodhart
(1960) reported that the mean corpuscular volume, mean cor-
puscular hemoglobin, mean corpuscular hemoglobin concentra-
tion, and the mean diameter were decreased when an iron de-
ficiency existed.

Numerous theories have been presented regarding the
absorption of iron. Copp and Greenberg (1946), and Widdow-
son and McCance (1937) have reported that most of the ab-
sorbed iron is conserved by the animal and very little is
excreted. Brown and Justus (1956) have shown that assimi-
lation is greatest in the duodenal region and progressively
less in the lower gastrointestinal tract. Moore et al.
(1944) demonstrated that ferric iron must be reduced to
the ferrous form before absorption can take place. Sharpe
et al. (1950) have shown by experimentally increasing bulk
or phytic acid content in the diet that there is a decrease

in the absorption of iron. Pirzio-Biroli et al. (1958)
reported that large amounts of ascorbic acid in the diet
will increase absorption. Hahn (1948) believes that absorp-
tion of iron is governed by the need for it. Balfour et al.
(1942) proposed that the mucosal cell contained an acceptor
mechanism for iron. Granick (1951) proposed that iron is
absorbed by combining with a protein apoferritin in the
mucosal cell to form ferritin which is absorbed through
the capillaries. When the mucosal cell becomes saturated,
absorption is decreased due to a "mucosal block." Brown
et al. (1958) believes the "mucosal block" theory should
be discarded. Hegsted et a1. (1952) reported rats gain
rapidly when large amounts of iron are present in the diet.
They suggested that when essentially all of the phosphate
was bound by iron absorption was very rapid. Recently Moore
et al. (1961) proposed ferritin to be a storage form of iron
in the mucosa and absorption to be controlled by the activ-
ity of the transport system which serves to pass iron across
a cell membrane. Elvehjen and Sherman (1932) reported that
c0pper does not affect the assimilation of iron. Heuk et
a1. (1946) found that when they added copper to the diet,
iron retention doubled.

Copp and Greenberg (1946) reported that small amounts
of iron are normally excreted. Greenberg et al. (1943) re-
ported that bile was the main pathway for the excretion of

iron. Known excretory routes are perspiration (Johnston

et al., 1950), feces (Greenberg et al., 1943), and urine;
(Heath, 1937).

Copper deficiency also produces a microcytic hypo-
chromic anemia (Smith et al., 1944; Foster, 1931). Cohen
et a1. (1927) were the first to report an interrelationship
between copper and iron in hemoglobin production. Chase
et a1. (1952), using radioiron, reported that copper facil-
itates iron absorption and hemoglobin synthesis in pigs.
Jensen et a1. (1956) reported that metabolism of iron was
not affected in copper deficient pigs but that absorption
was less. Because of the great deposits of iron that occur
in the livers of sheep and cattle on diets poor in copper,
Marston and Lee (1948) believe that copper does not have
any great influence on iron absorption in ruminants. Under-
wood (1962) believes that the evidence on the role of copper
in hemoglobin synthesis is very inadequate and that anemia
should be explained on the basis of impaired erythrocyte
maturation and a reduced survival time of the mature eryth-
rocyte produced.

Rimington (1959) proposed a scheme for the bio-
synthesis of heme (Figure l). Iodice et a1. (1958) reported
that anemia might be related to the lowering of8 -amino-
levulinic acid dehydrase which contains 0.1% copper. Later
Wilson et a1. (1959) reported that the removal of copper
did not affect this enzyme.

Drabkin (1951) reported that approximately 97-98%

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of an animal's iron requirement is supplied by catabolized
hemoglobin. He reasoned that the requirement for iron is
determined by the hemoglobin turnover rate and the amount
of iron accumulated during growth. In 1955, Walsh et al.,
cited by Underwood (1962) reported that at puberty human
males maintain higher hemoglobin values than do women; how-
ever, Byers et al. (1953) reported that this condition is
not true in cattle.

Knoop et al. (1935) reported that calves on a milk
diet were unthrifty, developed rough appearing hair, had
very low hemoglobin values, and in some, swollen joints and
stiffness appeared. Convulsions were seen in several ani-
mals at six months of age. Thomas et a1. (1953) reported
that calves fed a whole milk diet developed an anemia with
significant minimal hemoglobin values occurring during the
30th to 70th days. With one group of calves there was a
correlation between hemoglobin level and body weight gains.
Raleigh and Wallace (1961) reported that range calves reached
the minimal hemoglobin levels at approximately 105 days.
They postulated that the minimal values this late in calf-
hood were due to a diet of low quality forage. Swenson et
a1. (1957) reported that calves showed minimal hemoglobin
values beginning at 2 weeks and lasting until 8 weeks of age.

The data of Swenson et al. (1957) and Thomas et a1.
(1953) reveal a close correlation between body weight gains

and hemoglobin values between the second and eighth week.

Raleigh reports that treatment with iron and copper had no
significant effect on rate of gain. Carlson et a1. (1961)
reported that calves which received injectable iron showed
better weight gains at 10 weeks of age but the weights were
approximately the same as those of the non-medicated control

animals at 24 weeks of age.

CHAPTER III

MATERIALS AND METHODS

A--Experimental Animals

Forty-five Holstein male and female calves, all
less than 7 days of age, were obtained from dairy farmers
in the Lansing area. Before experimentation, each calf was
determined to be in good physical health on the basis of
a clinical examination. The animals were housed in the
Michigan State University dairy barn in individual pens on
sawdust bedding throughout the study. Pens were constructed

of plywood with metal bar doors covered with aluminum paint.

B--Experimental Procedures

Prior to experimentation, hemoglobin values were
determined for each calf. These values served as the basis
for dividing the calves into three experimental groups.
Hemoglobin values were arranged in a linear fashion and a
group was composed of fifteen calves selected from every
third value. Group III received 500 mg. (10 ml.) of iron-
dextran’ intramuscularly at the 7th day of the experiment

and again at the let day of the experiment. Calves were

 

I'Armidextran, Armour Pharmaceutical Company, Kan-
kakee, Illinois.

10

injected deeply into the posterior groups of muscles of a
rear leg. Group II received 30 mg. of iron sulfate powder‘
suspended in water, orally, each noon. Group I served as
the control.

The feeding regime was the same for all calves.
They were given whole milk at the level of 10% of body weight
daily during this study. Milk allotments were adjusted week-
ly. Water was available at all times. Beginning at the
tenth week of the experiment one pound of grain mixture“
was given daily. Third cutting alfalfa hay of good quality
was offered ad libitum beginning at the tenth week of the
experiment. Calves were weighed weekly.

Clotted blood samples for serum extraction and whole
blood samples for complete blood counts were collected once
a week from the jugular vein. Dipotassium Ethylenedine
Tetraacetate (E.D.T.A.P.)"‘ was used as the anticoagulant.

Serum samples were transferred into chemically“" cleaned

 

‘Ferrous Sulfate (fine crystal) FE $04.7H20, Lot
#3745. J. T. Baker Chemical Co., Phillipsburg, New Jersey.

uD-l6 Grain Ration--762 1/2 lbs. ground shelled corn,
627 lbs. crimped oats, 200 lbs. linseed meal, 133 lbs. soy
bean oil meal (44%), 100 lbs. corn distillers solubles, 133
lbs. dexmolasses, 20 lbs. dicalcium phosphate, 20 lbs. trace
mineral salt, 3/4 lb. Vit. A and D, and 3/4 lb. Vit. D-9F.

"'Dipotassium Ethylenedine Tetraacetate (E.D.T.A.P.).
Cambridge Chemical Products, Inc., 5850 Chase Road, Dearborn,
Michigan.

"**Preparation of 100 gms. sodium dichromate in 200
cc. water plus 800 cc. of commercial sulfuric acid.

11

glass vials. Serum was stored by freezing within twenty-
four hours after collection.

A Coulter Counter‘ was used for leukocyte and eryth-
rocyte enumeration. Differential leukocyte counts, retic-
ulocyte counts, hemoglobin (cyanmethemoglobin) and micro-
hematcrit procedures were done according to the manual "Out-
line for Veterinary Clinical Pathology."" Serum iron levels

were determined by the dipyridyl method of Ramsey (l962)."’

 

‘Coulter Electronics, Inc., Hialeah, Florida.

uMaxine M. Benjamin, The Iowa State University Press,
ARES , Iowa.

"*Harold Varley, Practical Clinical Biochemistry,
3rd edition, Interscience Publishers, 1962,.New York.

CHAPTER IV
RESULTS AND DISCUSSION

Prior to experimentation all calves were determined
to be in good health on the basis of a physical examination.
During the first three weeks of this study most calves de-
veloped pneumo-enteritis. Calves were treated for the con-
dition with systemic antibiotics (1/2 gm. neomycin plus 1
million units of aqueous penicillin) and neomycin calf scours
tablets (Biosol-M).' whenever a scouring condition was di-
agnosed, milk allotments were reduced by one-half until ces-
sation of scouring; then milk was gradually increased to
the normal amount over a three-day period.

Because of the sporadic outbreaks of calf salmonel-
losis near the Lansing area, fecal samples were taken for
bacteriologic culture. Calves were considered negative for
salmonella on the basis of these cultures.

Body weights of the calves were recorded weekly.

The three groups of calves showed weekly mean weights dif-
fering from each other in pattern. Calves receiving oral

iron showed a pattern similar to calves receiving no iron,
while calves injected with iron had mean body weights com—

paratively less the first 6 weeks and greater from the 8th

 

'Upjohn Company, Kalamazoo, Michigan.

12

13

to the 12th week than those calves receiving oral iron.

The mean body weights at the termination of the experiment

were very close for the three groups of calves and nearly

identical for the two groups receiving some form of iron.

There was no significant difference in body weights among

the three groups.

Table 1 0

Week

Initial

l

\DCJQO‘U'IDUJN

I'-'
O

11
12
13

Mean Body Weights of Calves, by Group

Group I

(No Iron)

99.2
104.9
113.0
123.8
133.5
144.7
156.1
169.8
183.6
195.4
206.8
217.6
228.0
240.4

1.
1.
1.
.1
.1
.1
.1
.1
.1
.1
.1
1.
.1

1.

3.98
4.04
4.28
4.61
4.71
5.07
5.16
5.39
5.58
6.07
6.36
6.80
7.06
7.66

(pounds)

Group II

(Oral Iron)

96.7
102.4
113.6
127.2
140.6
152.8
164.8
177.4
189.6
201.8
213.4
225.1
237.0
249.3

l+' I+ I+ l+ l+ l+ I+ I+ l+ H H H

|+

Group III

 

3.28
3.51
3.67
3.76
3.95
4.22
4.39
4.75
4.91
5.81
5.43
5.77
5.94
6.36

99.8
104.0
113.3
125.0
136.4
148.3
162.0
177.3
193.0
206.3
217.5
227.9
238.2
248.5

H H H I+

|+

l+ l+ l+ l+ l+ H H

|+

(Intramuscular

3.14
3.19
3.30
3.71
3.92
4.20
4.48
4.93
5.32
5.64
6.05
6.31
6.64
6.82

 

Average daily gains were calculated at the

end of

14

each week and are presented in Table 2. All the groups of
calves increased in average daily gain until about the 6th

to 7th week when they tended to level off. Calves receiv-
ing iron showed faster daily weight gains than did calves
receiving no iron. Raleigh (1962) reported that range calves
receiving iron and exposed to forage showed greater weight
gains from birth until 3 weeks and an increase in average

daily gain until about 6 weeks when they leveled off.

Table 2. Average Daily Gain at Weekly Intervals, by Group

 

(pounds)
Group III

7 days Group I Group II (Intramuscular
__;9 (N9 Iron) (Oral Icon) 1:92)

2 wks .82 .82 .61

3 wks .99 1.20 .97

4 wks 1.17 1.45 1.20

5 wks 1.23 1.57 1.31

6 wks 1.30 1.60 1.39

7 wks 1.36 1.50 1.48

8 wks 1.44 1.65 1.58

9 wks 1.51 1.66 1.66
10 wks 1.53 1.67 1.69
11 wks 1.54 1.67 1.68
12 wks 1.54 1.67 1.66
13 wks 1.53 1.67 1.65
14 wks 1.55 1.68 1.63

 

 

 

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w; a

2' {LA-

 

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s t.
_

 

 

 

' 15

A comparison was made within each group of calves
between the average total weight gain and the hemoglobin
(Hb.) level. Total weight gains of the seven calves with
initial low hemoglobin values were compared with the seven
calves that had high hemoglobin values. These data are pre-

sented in Table 3.

Table 3. Weight Gain in Comparison to Initial Hb. Level
(Pounds Per Day)

Groups of 1 wk. 3 wk. 6 wk. 9 wk.

3 o 6 w w o 2 wk
Hb. LQVQ].

‘No High Hb. 1.24;,15 1.541.53 1.90;.61 1.653359
Iron LOW Hb. 1007;074 1.50:049 1.82:065 1.48:064
nOral High Hb. 1.57;,32C 1.85;.4lb 1.80:,38 1.74:,21
Iron LOW Hb. 1.371.041 1.72:041 1076;045 1067:047
"'I.M.High Hb. 1.13;,41 1.6Q;.36 2.05;,51 1.46;,32
Iron Low Hb. 1.27;.51 1.85;.4213 2.14;.52a 1.61;.55

ESignificantly greater than least value (P < .05).

cSignificantly greater than least 2 values (P <L.05).
Significantly greater than least 2 values (P <..01).

 

Those calves having "high" hemoglobin values and

receiving either no iron or oral iron had greater total

 

No High Hb. Calf Nos. 3, 5, 6, 15, 31, 41, and 45
Iron Low Hb. Calf Nos. 1, 2, 14, 22, 33, 37, and 44

Oral High Hb. Calf Nos. 4, 7, 10, 16, 18, 24, and 27
Iron Low Hb. Calf Nos. 8, 12, 13, 19, 34, 35, and 38
.0.

I.M. High Hb. Calf Nos. 21, 29, 30, 32, 36, 40, and 43
Iron Low Hb. Calf Nos. 9, 11, 17, 25, 26, 39, and 42

16

weight gains than did the calves which had initial "low"
hemoglobin values. Calves which had initial "low" hemo-
globin values and received injectable iron had greater total
weight gains than did calves which had initial "high" hemo-
globin values. No explanation is offered for this phenom-
enon.

Blood samples were taken weekly for routine blood
counts. The mean hemoglobin values are shown in Table 4.
Hemoglobin values were significantly higher (P <1.01) for
the calves receiving oral and injectable iron when calves
were 21 days old. The average initial hemoglobin value
for all calves was 10.1 gm. per 100 m1. Raleigh and Wallace
(1962) reported that range calves receiving iron reached
minimal hemoglobin levels at about 105 days while Thomas
et a1. (1954) reported dairy calves showed a significant
hemoglobin increase beginning at the 20th day and gradually
increasing up to the termination at 41 days of age. The
calves in this experiment varied from previously reported
results. Animals in this study receiving no iron reached
a minimal hemoglobin value at 91 days of age (termination
of experiment). Calves receiving either oral or injectable
iron showed a significant hemoblobin increase over the group
of calves receiving no iron. Calves receiving oral iron
reached maximum hemoglobin readings at approximately 28

days of age and then tended to level off.

17

 

Table 4. Mean Hemoglobin Values for the Three Groups of
Calves
(Gm. per 100 M1.)
Treatment No Iron Oral Iron Intramuscular
jean.—
Age
Days
7 9.95 g .50 10.12 3; .46 10.03 3; .44
14 9.66 3; .53 10.05 3; .44 9.91 g .47
21 8.99 3; .47 10.16 ; .46" 9.83 3_-_ .51"
28 8.51 1 .44 10.43 3; .45" 9.94 3; .48"
35 9.29 1 .47 10.47 3; .47" 9.93 3; .49”
42 8.79 3; .44 10.55 3; .47" 10.00 1 .44"
49 8.71 3; .42 10.57 .1 .45“ 10.30 3, .44"
56 8.60 3; .45 10.46 1 .43" 10.63 3; .45"
63 8.79 3_ .49 10.45 3; .43“ 10.63 3; .48"
70 8.79 1 .48 10.55 3; .43" 10.41 3_- .43"
77 8.69 _+_ .43 10.47 .1 .46“ 10.32 3; .47"I
84 8.56 1 .45 10.53 3; .46" 10.33 3; .46"
91 8.24 3; .43 10.46 3; .44" 10.45 1 .49"

 

.. P<.01

18

Hubbert and Wallace (1959), using range calves, re-
ported a decrease in packed cell volume (P.C.V.) values un-
til calves reached the age of four weeks. Raleigh and Wal-
lace (1962), studying range calves in the state of Oregon,
found that P.C.V. values increased from birth to 3 weeks
and at each blood sampling date thereafter. In the work
presented here, calves showed a gradual increase in average
P.C.V. values from 7 days of age until they were 35 days
old. This difference was statistically significant (P«<.0.05)
from the fifth to eighth weeks for calves treated with oral
iron and at the fifth, seventh and eighth weeks for calves
treated with intramuscular iron. Average P.C.V. values are
presented in Table 5.

Red blood cell enumeration was done with the aid
of a Coulter counter. Average erythrocyte counts were not
statistically different among the experimental groups.
These data are presented in Table 6.

Mean corpuscular hemoglobin (M.C.H.) and mean cor-
puscular volume (M.C.V.) were calculated for each group of
calves using the mean values found for hemoglobin, hemato-
crit and erythrocytes. The following formulas were used.

M.C.H. Hb m er 1000 .
(micro-micrograms) R.B.C. 10

M.C.V. _ Volume pegged cells pgr 1000 cg,
(cubic microns) ' R.B.C. 10

 

19

 

 

 

Table 5. Average Packed Cell Volume by Calf Group
P.C.V. (%)

Age Intramuscular
mus NW 231% Lem...
7 33.8 3; 1.4 34.3 3; 1.5 33.2 3; 1.3
14 33.9 3; 1.4 33.5 3; 1.4 33.2 3; 1.3
21 33.7 3; 1.3 34.7 3; 1.4 35.7 3; 1.5
28 33.5 3; 1.1 36.0 3; 1.3 37.3 3; 1.4
35 33.1 3; 1.2 38.2 3; 1.4‘ 38.5 3; 1.3‘
42 34.0 3; 1.3 38.5 3, 1.5‘ 37.7 3; 1.3
49 33.7 3; 1.4 38.3 3; 1.5' 37.9 3, 1.3‘
56 33.7 3; 1.4 37.9 3; 1.5’ 37.7 3; 1.3‘
63 34.4 3 1.5 38.0 3 1.5 36.73; 1.4
70 34.0 3; 1.3 38.7 3 1.4 36.3 3 1.2
77 33.7 3; 1.4 37.3 3; 1.6 36.5 3; 1.3
84 33.8 3 1.3 37.9 3; 1.5' 36.1 3, 1.1
91 34.0 3. 1.3 37.8 3; 1.4 36.5 3, 1.0

 

’ P <1.05

20

Table 6. Mean Erythrocyte Values for Control and Iron
Treated Calves

 

 

 

Age (106 per mm'3) Intramuscular

,Qéyg N9 grog 03a; Eggn Iggg
7 8.07 3 .27 8.22 3; .33 8.58 3 .26
14 8.08 3; .26 8.24 3 .31 8.56 3 .24
21 8.12 3 .27 8.21 3 .31 8.58 3 .26
28 8.13 3 .29 8.18 3; .33 8.63 3; .27
35 8.09 3 .29 8.19 3 .31 8.54 3 .30
42 8.11 3 .29 8.27 3 .33 8.45 3 .27
49 8.16 3 .3 8.24 3 .3 8.48 3 .29
56 8.17 3 .3 8.27 3; .3 8.53 3 .24
63 8.20 3 .3 8.26 3 .3 8.51 3 .22
70 8.33 3 .3 8.27 3 .29 8.41 3 .30
77 8.30 3 1.02 8.26 3 .33 8.72 3 .25
84 8.30 3 .3 8.22 3 .33 8.58 3 .27
91 8.32 3 .3 8.19 3 .33 8.60 3 .27

 

Calculated blood indices are given in Table 7. Mean
corpuscular hemoglobin values decreased gradually in calves
which received no iron. Calves treated with iron showed
a very slight increase in M.C.H.; however, these values re-
mained somewhat constant. Mean corpuscular volume values
showed a gradual increase in calves treated with supple-

mental iron.

Table 7 0

Calculated Blood Indices by Calf Group

M.C.H.'(micro-micrograms)

Age

No

Oral

Im.

21

M.C.V.

NO

Oral

(cubic microns)

Im.

.2SIi___ADaaL___JDEHL___JBEmL______I£QB.___LEMB___JDEHL

7 12.32 12.31 11.68 41.9 41.8 38.8
14 11.95 12.19 11.57 41.9 40.6 38.8
21 11.07 12.37 11.45 41.5 42.3 41.6
28 10.46 12.75 11.51 41.2 44.0 43.2
35 11.48 12.78 11.62 40.9 46.6 45.1
42 10.83 12.75 11.83 41.9 46.6 44.7
49 10.67 12.82 12.14 41.3 46.5 44.7
56 10.52 12.64 12.46 41.2 45.8 44.2
63 10.71 12.65 12.49 42.0 46.0 43.2
70 10.55 12.75 12.37 40.8 46.7 43.1
77 10.46 12.67 11.83 40.6 45.1 41.9
84 10.31 12.81 12.03 40.7 46.1 42.1
91 9.90 12.77 12.15 40.9 46.2 42.5

 

The percentage of reticulocytes observed was in-
creased in the calves which received iron. Calves receivb
ing oral iron showed a somewhat gradual and uniform increase
over calves receiving no iron. The calves receiving intra-
muscular iron showed a definite increase in reticulocyte
percentage from the 2nd to 5th weeks which was immediately
after the iron injections. Table 8 and Figure 1 summarize

the reticulocyte findings.

22

Table 8. Average Reticulocyte Values for the Three Groups

 

 

of Calves
Number Per 100 Erythrocytes
Age Intramuscular
1331,33 MWn 11:211—
7 0.7 1.0 .7
14 0.8 1.2 8.0
21 0.5 1.3 4.4
28 0.6 1.4 5.2
35 0.4 1.5 1.2
42 0.5 1.3 .4
49 0.5 1.6 .4
56 0.4 1.2 .24
63 0.4 1.1 .2
70 0.4 1.1 .33
77 0.5 1.3 .28
84 0.4 1.1 .3
91 0.4 1.3 .28

 

Mean values for serum iron content are presented
in Table 9. Planas and DeCastro (1960) found serum iron
levels for adult cattle to be 142.4 3,22.l micrograms.with
a range of 95-224jugm. per 100 ml. _The results of this
study differ somewhat from the values established for the
adult.

Number Reticulocytes per 500 Erythrocytes

40

35

30

25

20

23

Reticulocyte Response from Iron Treatment,
for the Three Groups of Calves

 

 

 

‘ A
.. I)
i \ No iron
_ ’ \ -—--—-- Oral iron
’ \ //\ _ _ I.M. iron
" \
x \
l \
J
' \
: \ ,/'\.
‘l // T\, / \.
’ K / i V \.\ ,I\. J/'
‘ /' \ .\" d‘/ \./
\

 

 

 

i i 1*, I ‘I’ I I I I l r‘ I
7 14 21 28 35 42 49 56 63 70 77 -84 91

Days of Age

 

24

Mean Serum Iron Values by Calf Group

(ugm. Per 100 M1.)

 

Table 9.

Age

0313 N9 1:93
7 96.1 3 6.8
14 92.1 3 7.0
21 93.1 3 7.8
28 94.1 3 6.9
35 92.3 3 7.2
42 96.5 3 7.0
49 95.7 3 6.8
56 97.6 3 7.5
63 95.5 3 7.1
70 101.5 3 7.0
77 97.9 3 7.1
84 103.0 3 7.6
91 99.4 3 7.5

 

Intramuscular
2221.122}; 12911——

96.8 3 6.0 97.8 3 8.8‘
123.7 3 6.5" 149.0 3 8.0"
149.8 3 5.1" 194.3 3 8.8"
149.0 3 6.0.” 198.9 3 7.5"
146.5 3 6.0“ 198.7 3 7.3“
148.8 3 5.4" 193.6 3 8.1"
152.2 3 7.0" 200.8 3 7.2"
152.3 3 6.7"I 199.5 3 8.5”
154.7 3 6.4" 204.7 3 9.8“
155.7 3 6.2" 195.5 3 9.4"
153.9 3 5.9” 188.1 3 10.1"
158.1 3 6.5" 192.8 3 10.2”
156.2 3 6.3“ 186.3 3 8.9"

 

" P <.01

25

A significant difference was seen in serum iron
levels immediately after the initial iron treatment. Serum
iron levels remained constant for calves which received no
iron throughout the experiment.

Leukocyte enumeration and differential counting was
performed weekly throughout this experiment. These data
are presented in the appendix. Values from leukocyte stud-
ies were essentially normal. Iron administration had no
apparent effect on either the total or differential leuko—
cyte counts; however, this determination was valuable from

the standpoint of evaluating the health status of the calves.

1.

2.

3.

4.

5.

6.

7.

V. CONCLUSIONS

Calves which received iron showed no statistically sig-
nificant difference in body weight gains when compared
to calves receiving no iron.

A difference in total weight gains was seen depending

on the iron treatment. The group of calves which re-
ceived oral iron and had higher hemoglobin values had
greater total weight gains while the calves which re-
ceived intramuscular iron and had low hemoglobin levels
had greater total weight gains.

Calves which received iron supplement, either oral or
intramuscular, showed statistically significant increases
in hemoglobin levels and packed cell volumes as compared
to calves receiving no iron.

Iron supplement, either oral or intramuscular, quickly
raised and maintained serum iron levels to a significantly
higher level in the young calf in this study.

A gradual and uniform increase in reticulocyte response
was observed in the calves which received oral iron.
Calves receiving injectable iron exhibited a rapid and
marked increase in reticulocytes which was sustained,
however, only during the treatment period.

The physiological anemia, characteristic of most mammals,
was not observed in calves on a whole milk diet when

an iron supplement was employed.

26

APPENDIX

28

TABLE 1. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 1

Age W
(days) S.I. Hc. Hb. RBC Ret. WBC N L M E B B.W.
initial 121
7 114 33 9.1 7.79 7 13.17 38 62 0 0 0 130
14 115 34 8.6 7.83 4 11.6 43 53 0 4 0 143
21 119 33 7.8 7.73 5 8.4 27 70 0 2 1 159
28 108 34 7.2 7.88 8 9.3 37 63 0 0 0 171
35 110 33 8.4 7.85 0 7.6 46 53 0 1 0 185
42 125 32 7.0 7.77 0 7.4 41 55 l 3 0 199
49 114 32 7.4 7.70 8 7.8 39 59 0 2 0 214
56 118 32 7.2 7.65 0 7.9 28 71 0 1 0 227
63 110 33 7.6 7.72 2 8.1 35 60 0 5 0 242
70 109 32 7.0 7.80 0 7.4 39 61 0 0 0 255
77 121 32 8.2 7.73 3 7.2 33 67 0 0 0 269
84 117 33 7.4 7.88 1 7.3 41 57 0 2 0 283
91 118 31 7.1 7.91 5 7.2 47 51 0 2 O 299

 

TABLE 2. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 2'

 

Age D r a
(days) S.I. HC. Hb. RBC Ret. WBC N L M E B B.W.
initial 88
7 68 32 9.0 7.96 7 11.6 58 42 0 0 0 98
14 60 33 9.0 8.05' 0 13.5 51 39 0 0 0 107
21 57 32 8.4 8.24 1 14.2 32 68 0 0 0 118
28 66 33 7.8 8.37 14 10.8 47 51 1 1 0 129
35 58 33 8.6 7.79 0 12.7 43 66 1 0 0 141
42 61 33 8.4 7.87 0 8.3 40 57 1 2 0 155
49 72 33 8.0 7.96 0 5.4 51 49 0 0 0 171
56 77 32 7.8 7.90 0 6.2 47 52 0 0 1 186
63 73 31 8.2 8.05 '2 6.8 38 59 O 3 0 200
70 70 33 8.6 8.11 9 6.8 40 60 0 0 0 215
77 71 33 8.0 8.07 1 8.3 37 63 O 0 O 229
84 79 32 8.0 8.12 4 9.1 52 47 1 O 0 238
91 74 32 8.2 7.99 0 8.1 43 67 0 1 0 250
S.I. 2 Serum Iron (micrograms/100 ml. blood)
HC. 3 Hematocrit (percent)
Hb. a Hemoglobin (grams/100 ml. blood)
RBC = Red blood cell (105)
Ret. = Reticulocyte (numbergSOO erythrocytes)
WBC a White blood cell (10 )
Differential
N - Neutrophil (%)
L - Lymphocyte (%)
M . Monocyte (%)
E = Eosinophil (%)
B - Basophil (%)
B.W. a Body weight (pounds)

29

 

 

TABLE 3. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 3
Age D f
(days) S.I. Hc. Hb. RBC Ret. WBC N L M E B B.W.
initial 115
7 141 35 10.1 8.53 0 14.0 41 58 0 1 0 121
14 138 35 10.0 8.49 3 17.2 38 62 0 0 0 130
21 144 35 9.8 8.52 2 16.1 44 56 0 0 0 143
28 151 34 9.0 8.62 2 11.6 50 50 0 0 0 158
35 150 34 9.4 8.77 O 9.8 48 52 0 0 0 174
42 154 34 9.2 8.66 3 10.4 46 54 0 0 0 187
49 139 33 9.2 8.52 O 5.7 39 61 0 O 0 203
56 141 34 9.2 8.56 5 8.1 37 63 0 0 0 220
63 140 35 9.0 8.62 1 9.4 44 56 0 0 0 237
70 145 34 8.6 8.51 O 8.5 51 49 0 0 O 252
77 147 34 9.4 8.56 8 8.0 54 45 0 1 0 266
84 161 34 9.0 8.69 5 8.6 47 53 0 0 0 283
91 152 33 8.9 8.74 0 7.1 38 62 0 0 0 301
TABLE 4. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 4
Age D
(days) S.I. Hc. Hb. RBC Ret. WBC N L M E s B.W.
initial 75
7 98 37 11.4 9.22 1 8.9 37 63 0 O 0 79
14 127 37 11.8 9.14 12 11.8 22 78 0 0 0 89
21 148 39 11.6 9.08 3 7.4 39 61 0 0 0 107
28 151 41 12.2 8.97 1 8.2 28 72 0 0 0 123
35 146 44 12.4 9.06 2 13.0 23 77 0 0 0 138
42 159 43 12.6 9.15 6 9.1 25 75 0 0 0 150
49 150 43 12.0 9.22 0 8.6 31 69 0 0 0 163
56 162 41 11.6 9.19 3 8.5 37 63 0 0 0 176
63 171 42 11.8 9.17 5 8.0 35 65 0 0 0 190
70 158 43 12.0 9.25 4 9.1 30 69 0 l 0 201
77 162 42 11.6 9.03 0 8.3 38 62 0 0 0 213
84 163 44 11.8 9.11 4 9.9 39 60 0 l 0 227
91 168 42 11.8 9.23 8 10.4 42 58 0 0 0 240
S.I. = Serum Iron (micrograms/100 ml. blood)
Hc. = Hematocrit (percent)
Hb. = Hemoglobin (grams/100 ml. blood)
RBC a Red blood cell (105)
Ret. = Reticulocyte (number/500 erythrocytes)
WBC = White blood cell (103)
Differential
N a Neutrophil (%)
L = Lymphocyte (%)
M a Monocyte (%)
E a EosinOphil (%)
B a Basophil (%) ,
B.W. a Body weight (pounds)

3O

 

 

TABLE 5. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 5
Age Diffgrgggial
(days) S.I. Hc. Hb. RBC Ret. WBC N L M E B B.W.
initial 98
7 90 37 11.1 9.10 1 6.3 40 60 0 0 0 106
14 84 37 12.2 9.04 0 5.8 44 56 O 0 0 115
21 96 36 9.8 9.03 9 8.6 41 59 0 0 0 126
28 78 37 9.4 8.95 0 12.1 52 48 0 0 0 134
35 81 38 11.0 9.08 O 9.2 58 42 0 0 0 144
42 84 39 10.2 9.11 2 8.7 50 $0 0 0 0 154
49 92 39 10.4 9.17 1 10.1 46 47 2 5 0 165
56 96 37 9.6 9.12 0 6.8 48 49 1 2 0 177
63 99 37 10.4 8.91 4 9.4 39 60 1 0 0 186
70 106 39 10.6 8.93 0 10.1 47 51 2 0 0 200
77 95 37 10.0 9.00 1 6.7 44 55 1 0 0 214
84 98 37 10.0 9.12 0 8.9 44 55 0 1 0 226
91 109 36 9.5 9.14 1 8.1 44 59 1 0 0 240
TABLE 6. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 6
Age Dgfifieggngzgl
(days) S.I. HC. Hb. RBC Ret. WBC N L M E B B.W.
initial 84
7 90 36 9.5 8.55 2 7.8 37 63 0 0 0 90

14 92 36 9.2 8.62 2 8.3 47 53 0 0 0 99
21 100 36 8.0 8.67 0 9.4 41 57 0 2 0 107
28 97 36 7.8 8.63 4 13.3 39 58 0 3 0 116
35 98 36 9.5 8.48 1 14.2 54 44 0 2 0 124
42 103 37 8.6 8.52 0 11.7 46 52 0 2 0 134
49 99 36 8.0 8.55 0 10.8 48 50 0 2 0 146
56 109 37 8.1 8.69 7 11.0 46 53 0 1 0 157
63 96 37 8.0 8.62 2 9.7 39 61 O 0 0 168
70 115 36 8.4 8.58 0 8.7 43 53 0 0 0 180
77 98 36 8.0 8.44 4 9.3 44 54 0 2 O 189
84 111 37 8.0 8. 0 9.5 40 57 0 3 0 203
91 104 37 7.5 8.49 3 10.4 48 50 0 2 0 215

S.I. = Serum Iron (micrograms/100 ml. blood)

Hc. = Hematocrit (percent)

Hb. = Hemoglobin (grams/100 ml. blood)

RBC = Red blood cell (106)

Ret. - Reticulocyte (numberéSOO erythrocytes)

WBC - White blood cell (10 )

Differential

a Neutrophil (%)
Lymphocyte (%)
Monocyte (%)
Eosinophil (%)
Basophil (%)

Body weight (pounds)

uamzzt*z

B.W.

31

 

 

TABLE 7. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO.
Age W
(days) S.I. HC. Hb. RBC Ret. WBC N L M E B B.W.
initial 93
7 90 38 11.7 9.18 2 7.8 49 51 0 0 0 97
14 112 37 11.4 9.23 11 12.4 56 44 O 0 0 109
21 151 37 11.8 9.21 0 8.2 62 38 0 0 0 124
28 148 38 12.3 9.06 4 9.4 47 52 0 1 0 137
35 139 39 12.6 9.10 6 9.2 47 53 0 0 0 150
42 146 42 12.8 9.33 6 9.4 45 55 0 0 0 161
49 141 44 12.6 9.06 8 9.6 49 51 0 0 0 175
56 144 41 12.1 9.11 2 7.2 38 60 0 0 2 187
63 154 42 12.2 8.85 5 8.4 40 58 0 2 0 197
70 150 42 12.4 8.67 2 9.8 39 57 0 4 0 209
77 153 41 12.2 9.23 6 9.9 37 63 0 0 O 218
84 161 41 12.6 9.25 16 10.4 47 51 0 2 0 232
91 152 40 12.1 9.34 7 8.3 50 50 0 0 0 244
TABLE 8. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO.
Age W
(days) 3.1. Hc. Hb. RBC Ret. WBC N L M E s B.W.
initial 99
7 111 31 8.6 7.95 4 11.1 53 47 0 0 0 102
14 139 31 8.6 8.30 9 12.6 51 49 0 0 0 110
21 171 33 8.6 8.38 9 9.4 49 50 0 1 0 121
28 160 35 9.0 8.24 15 9.4 49 50 0 1 0 131
35 161 36 8.8 8.41 0 10.1 46 53 0 1 0 140
42 159 34 9.0 8.46 7 11.4 39 61 0 0 0 151
49 181 35 9.4 8.10 12 8.9 33 66 0 1 0 163
56 170 35 9.2 8.21 11 9.0 38 62 0 0 0 173
63 172 35 9.2 8.25 7 10.2 40 58 0 2 0 185
70 168 35 9.4 8.39 13 7.8 47 53 0 0 0 195
77 169 34 9.0 8.32 7 9.4 39 61 0 0 0 206
84 176 34 9.8 8.37 10 10.0 40 60 0 0 0 215
91 161 33 9.8 8.30 2 9.7 40 60 0 0 0 225
S.I. = Serum Iron (micrograms/100 ml. blood)
Hc. = Hematocrit (percent)
Hb. = Hemoglobin (grams/100 ml. blood)
RBC = Red blood cell (105)
Ret. = Reticulocyte (number/500 erythrocytes)
WBC . White blood cell (103)
Differential
N = Neutrophil (%)
L = Lymphocyte (%)
M = Monocyte (%)
E a Eosinophil (%)
B a Basophil (%)

B.W.

Body weight (pounds)

7

8

32

 

 

TABLE 9. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 9
Age D
(days) S.I. Hc. Hb. REC Ret. WBC N L M E E B.W.
initial 110
7 110 24 7.5 7.56 2 8.4 38 62 0 0 O 118
14 154 28 7.4 7.28 32 5.3 36 64 0 0 0 131
21 198 27 7.2 7.31 29 4.8 43 57 O 0 0 146
28 206 29 7.3 7.40 30 8.9 40 59 0 l 0 160
35 198 31 7.4 6.96 9 10.2 42 58 0 0 0 173
42 197 29 8.0 7.20 4 7.8 39 61 0 0 0 189
49 211 29 8.2 7.72 2 8.6 37 60 0 3 0 207
56 205 30 8.1 7.28 1 8.8 40 60 0 0 0 224
63 202 28 8.0 7.44 0 9.0 48 49 0 2 1 240
70 183 29 8.2 7.93 0 10.1 46 54 0 0 0 254
77 195 29 8.0 8.44 2 9.6 42 58 0 0 0 266
84 197 30 7.8 8.27 1 7.3 47 53 0 0 0 280
91 192 30 7.7 7.22 0 8.2 48 51 0 1 0 294
TABLE 10. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 10
Age D
(days) S.I. Hc. Hb. RBC Ret. WBC N L M E B B.W.
initial 108
7 86 40 11.7 9.26 10 7.1 62 38 0 O 0 116
14 104 40 11.4 9.36 7 6.7 57 43 0 0 0 127
21 140 41 11.8 9.30 7 12.2 68 32 0 0 0 141
28 131 43 12.0 9.18 9 8.4 59 39 0 2 0 154
35 138 44 12.0 9.41 12 9.3 53 46 0 1 0 165
42 135 44 12.0 9.48 14 14.1 56 43 0 1 O 179
49 146 42 12.0 9.50 8 8.2 55 41 0 4 O 193
56 131 42 11.8 9.06 8 9.4 60 40 0 0 0 207
63 140 42 11.6 9.23 6 10.1 59 39 0 2 0 221
70 139 43 12.0 9.36 6 8.2 61 37 0 2 0 234
77 146 43 12.2 9.47 5 7.4 56 44 0 0 0 246
84 148 43 11.6 9.12 4 6.9 52 45 0 3 0 256
91 151 44 11.9 9.32 0 8.0 60 40 0 0 0 271
S.I. = Serum Iron (micrograms/100 ml. blood)
Hc. = Hematocrit (percent)
Hb. = Hemoglobin (grams/100 ml. blood)
RBC = Red blood cell (106)
Ret. a Reticulocyte (number/500 erythrocytes)
WBC . White blood cell (103)
Differential
N a Neutrophil (%)
L a Lymphocyte (%)
M = Monocyte (%)
E . Eosinophil (%)
B = Basophil (%)
B.W. a Body weight (pounds)

33

TABLE 11. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 11

Age D
(days) S.I. Hc. Hb. RBC Ret. WBC N L M E B B.W.
initial 105
7 82 25 6.2 7.68 3 8.5 52 48 0 0 0 104
14 148 23 6.4 7.51 .54 9.3 56 44 0 0 0 112
21 173 24 6.0 7.56 28 7.6 39 61 0 0 0 123
28 189 27 6.6 7.28 24 14.8 40 59 0 1 0 135
35 188 28 6.4 7.32 5 9.9 45 55 0 0 0 146
42 178 28 6.6 7.47 5 10.6 40 60 0 0 0 158
49 197 30 6.8 7.33 4 11.7 38 62 0 0 0 172
56 179 29 7.4 7.71 2 9.3 42 57 0 0 1 187
63 201 28 7.1 7. 3 15.3 44 56 0 0 0 197
70 190 28 7.3 7.15 2 14.3 37 61 0 2 0 199
77 188 28 7.0 7.71 2 10.2 40 60 0 0 0 211
84 180 30 7.2 6.98 2 9.6 42 58 0 0 0 220
91 174 29 6.7 7.31 3 12.1 45 55 0 0 0 228

 

TABLE 12. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 12

 

Age D f

(days) S.I. Hc. Hb. REC Ret. WBC N L M E B B.W.
initial 92

7 96 30 7.8 5.82 12 9.5 61 39 0 0 0 96

14 126 29 7.6 6.30 0 12.1 65 35 0 0 0 109
21 139 30 7.6 6.06 10 18.2 63 37 0 0 0 124
28 143 31 7.6 5.88 13 9.4 66 43 0 1 0 138
35 152 34 7.6 6.04 8 10.1 65 35 0 0 0 150
42 152 34 7.8 5.93 7 9.8 59 40 0 l 0 164
49 158 34 7.6 6.30 9 10.9 61 38 0 1 0 181
56 161 32 8.0 6.18 10 9.6 58 40 0 2 0 193
63 171 30 7.8 6.08 8 12.2 60 39 0 1 0 207
70 182 32 8.0 6.26 11 11.8 55 44 0 1 0 220
77 158 34 8.0 6.07 17 13.2 58 41 1 0 0 235
84 163 33 8.0 6.17 3 9.8 56 43 0 1 0 247
91 160 33 8.1 6.06 11 12.1 59 40 1 0 0 261

S.I. = Serum Iron (micrograms/100 ml. blood)

Hc. - Hematocrit (percent)

Hb. a Hemoglobin (grams/100 ml. blood)

RBC - Red blood cell (105)

Ret. = Reticulocyte (numberéSOO erythrocytes)

WBC a White blood cell (10 )

Differential

Neutrophil (%)

a Lymphocyte (%)

= Monocyte (%)

a Eosinophil (%)

- Basophil (%)

B.W. - Body weight (pounds)

mumbz

34

 

 

TABLE 13. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 13
Age _Euééssseiiel_
(days) S.I. HC. Hb. RBC Ret. WBC N L M E B B.W.
initial 96
7 101 33 9.8 8.02 1 7.6 61 39 0 0 0 101
14 136 32 9.6 7.96 13 5.4 59 41 0 0 0 110
21 152 35 9.8 7.85 12 6.1 63 37 O 0 0 125
28 153 35 10.0 7.88 8 7.8 50 50 0 0 0 138
35 163 39 9.8 8.03 13 6.4 64 36 0 0 0 148
42 150 37 10.2 8.10 2 6.8 57 63 0 0 0 162
49 157 37 10.2 7.93 14 8.4 56 44 0 0 0 174
56 141 37 10.0 7.83 2 9.3 52 48 0 0 0 185
63 155 38 9.8 8.07 9 7.8 53 47 0 0 0 196
70 157 38 10.4 8.31 10 6.1 49 51 0 0 0 206
77 150 35 10.6 8.12 7 9.6 61 39 0 0 O 218
84 146 35 10.6 7.88 8 8.3 57 42 0 1 0 230
91 148 37 10.8 7.96 8 9.4 56 44 0 0 0 242
TABLE 14. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 14
Age Di
(days) S.I. HC. Hb. RBC Ret. WBC N L M E B B.W.
initial 113
7 92 25 6.8 6.40 0 11.4 69 31 0 0 O 113
14 81 25 5.8 6.48 1 14.9 64 36 0 0 0 117
21 80 25 6.4 6.53 2 13.1 59 41 0 0 0 124
28 94 26 6.0 6.47 2 9.6 67 33 0 0 0 130
35 72 25 6.2 6.63 9 12.4 62 38 0 0 0 138
42 88 26 6.2 6.71 1 11.7 70 29 0 1 0 145
49 79 26 6.2 6.88 7 9.8 59 40 0 1 0 153
56 76 26 6.0 6.55 0 12.1 56 41 0 3 0 161
63 70 27 5.8 6.58 5 11.4 53 45 0 2 0 169
70 88 26 6.2 7.20 1 8.3 57 40 l 2 0 175
77 93 25 6.2 6.57 6 9.1 60 39 0 1 0 182
84 90 26 6.0 6.61 2 10.9 58 40 0 2 0 189
91 91 26 5.8 6.53 0 64 33 0 3 0 198
S.I. 2 Serum Iron (micrograms/100 ml. blood)
Hc. = Hematocrit (percent)
Hb. - Hemoglobin (grams/100 ml. blood)
REC - Red blood cell (106)
Ret. - Reticulocyte (number/500 erythrocytes)
WEC . White blood cell (103)
Differential
N a Neutrophil (%)
L - Lymphocyte (%)
M a Monocyte (%)
E a Eosinophil (%)
B - Basophil (%)
B.W. a Body weight (pounds)

35

TABLE 15. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 15

Age .21££s£223121_
(days) S.I. Hc. Hb. RBC Ret. WBC N L M E B B.W.
initial 76
7 129 39 11.5 9.33 10 9.8 65 35 0 0 0 82
14 135 39 11.0 9.27 18 9.6 68 32 0 0 0 91
21 138 39 10.6 9.36 5 8.5 67 33 0 O 0 104
28 126 37 9.6 9.44 0 8.3 62 38 0 0 O 116
35 131 36 11.8 9.51 0 11.3 58 41 0 0 1 129
42 128 38 10.4 9.33 9 10.1 63 37 0 0 0 142
49 141 38 10.0 9.40 0 9.6 57 43 0 0 0 157
56 139 39 10.0 9.51 1 9.4 58 42 0 0 0 171
63 137 40 10.8 9.45 3 9.0 59 41 0 0 0 184
70 141 39 10.4 10.40 0 7.8 60 40 0 O 0 197
77 130 39 9.8 10.02 5 8.8 59 41 0 0 0 207
84 140 40 9.5 9.92 5 9.4 57 43 0 0 0 221
91 144 40 9.0 9.97 3 10.1 63 37 0 0 0 234

 

TABLE 16. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 16

 

Age D

(days) S.I. Hc. Hb. RBC Ret. WBC N L M E B B.W.
initial 87

7 99 42 13.0 9.69 8 7.8 56 44 O 0 0 93

14 136 41 13.0 9.80 3 12.6 62 37 0 1 0 102
21 160 42 12.8 9.77 10 9.3 55 45 0 0 0 113
28 153 13.4 9.87 0 14.1 50 50 0 0 0 126
35 144 46 13.2 9.83 8 13.3 46 52 0 2 0 137
42 154 45 13.6 9.96 10 10.9 43 54 0 1 2 146
49 168 47 14.0 9.80 7 7.8 53 41 1 0 3 155
56 167 46 13.8 9.92 11 12.1 54 44 2 0 0 167
63 148 45 13.8 10.07 14 8.4 55 43 0 2 0 174
70 149 47 13.4 9.79 5 9.3 59 41 0 0 0 185
77 151 45 13.6 9.84 4 11.1 58 40 0 0 2 195
84 163 47 13.8 9.97 0 9.3 63 37 0 0 0 207
91 161 46 13.6 9.73 14 10.4 58 39 0 3 0 218

S.I. = Serum Iron (micrograms/100 ml. blood)

Hc. - Hematocrit (percent)

Hb. = Hemoglobin (grams/100 ml. blood)

RBC 3 Red blood cell (105)

Ret. = Reticulocyte (number/500 erythrocytes)

WEC e White blood cell (103)

Differential

N a Neutrophil (%)
L = Lymphocyte (%)
M = Monocyte (%)
E a Eosinophil (%)
B . Basophil (%)
B.W. a Body weight (pounds)

36

 

 

TABLE 17. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO.
Age D'
(days) S.I. Hc. Hb. RBC Ret. WBC N L M E B B.W.
initial 107
7 60 28 10.2 8.85 5 14.2 61 39 0 0 0 110
14 121 26 10.0 9.25 48 12.4 45 55 0 0 0 118
21 168 32 9.6 9.42 22 9.0 58 41 0 1 0 130
28 165 33 10.0 9.29 41 8.1 39 60 0 1 0 141
35 176 35 10.4 9.21 5 7.6 42 58 0 0 O 154
42 163 35 10.8 9.19 2 5.4 42 55 1 0 2 166
49 170 35 10.8 9.36 4 9.4 45 55 0 0 0 182
56 174 34 11.0 8.97 0 8.6 46 51 l 2 0 197
63 183 33 11.0 9.25 1 12.1 48 48 l 0 3 211
70 158 33 11.0 9.34 3 10.8 53 47 0 0 0 227
77 159 29 10.6 9.53 0 7.8 47 49 0 4 0 232
84 179 33 10.6 9.12 O 9.5 46 53 0 0 1 240
91 163 33 10.8 8.97 0 10.3 49 51 0 0 0 251
TABLE 18. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO.
Age Diff§£§g§;§1
(days) S.I. Hc. Hb. REC Ret. WEC N L M E B B.W.
initial 107
7 91 35 10.4 8.58 0 8.4 63 37 0 0 0 114
14 116 34 10.6 8.69 5 9.6 60 39 0 l 0 127
21 151 36 10.2 8.81 10 10.8 70 29 0 1 0 142
28 148 37 9.8 8.60 14 9.5 73 27 0 0 0 159
35 149 40 9.8 8.59 7 9.0 70 29 0 1 O 175
42 153 39 10.0 8.46 0 9.8 57 41 1 0 1 187
49 147 38 10.0 8.69 6 7.3 58 40 2 0 0 201
56 144 39 10.0 8.75 0 8.1 62 38 0 0 0 215
63 153 41 10.4 8.67 5 9.8 63 37 0 0 0 3227
70 143 39 9.8 8.63 9 12.1 58 41 1 0 0 241
77 162 39 9.0 9.08 4 9.6 59 39 0 2 0 255
84 162 38 9.4 8.96 6 10.4 64 36 0 0 0 268
91 171 38 9.0 8.79 7 11.1 61 38 0 0 1 282
S.I. - Serum Iron (micrograms/100 ml. blood)
Hc. = Hematocrit (percent)
Hb. = Hemoglobin (grams/100 ml. blood)
RBC 2 Red blood cell (106 )
Ret. = Reticulocyte (number/500 erythrocytes)
WBC = White blood cell (103 )
Differential
N a Neutrophil (%)
L a Lymphocyte (%)
M = Monocyte (%)
E = Eosinophil (%)
B = Basophil (%)

Body weight (pounds)

17

18

37

 

 

20

TABLE 19. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 19
Age D;ffereg;;31
(days) 8.1. Hc. Hb. RBC Ret. WBC N L M E B B.W.
initial 91
7 102 34 10.1 8.24 4 5.6 62 38 0 0 0 98
14 133 33 9.6 8.11 10 8.1 64 36 0 0 0 107
21 154 35 9.6 8.10 1 8.6 58 41 0 0 1 119
28 162 36 9.9 8.11 9 10.4 55 44 1 0 0 128
35 151 38 9.8 8.30 5 11.3 63 37 0 0 0 140
42 151 39 9.8 8.38 12 9.8 65 33 0 2 0 150
49 169 38 10.0 8.25 7 8.4 59 41 0 0 0 159
56 163 38 10.3 8.19 5 12.6 58 42 0 0 0 171
63 150 38 10.2 8.16 8 9.4 52 44 0 1 3 183
70 168 38 10.4 8.25 3 8.4 58 41 1 0 0 195
77 161 36 9.8 8.12 4 7.8 65 35 0 0 0 206
84 164 36 10.0 8.25 9 9.3 58 39 0 3 0 217
91 166 37 9.7 8.19 0 10.3 61 38 0 1 0 226
TABLE 20. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO.
Age D
(days) 5.1. He. Hb. REC Ret. WBC N L M E B B.W.
initial 92
7 56 30 10.1 8.96 6 9.8 72 28 0 0 0 93
14 108 32 10.2 8.84 44 10.1 70 29 0 1 O 104
21 135 30 9.5 8.88 34 9.6 73 76 0 1 0 117
28 160 33 10.3 9.08 21 9.9 91 9 0 0 0 129
35 157 35 9.6 9.06 4 9.8 88 11 1 0 0 140
42 141 34 10.1 9.02 0 11.7 68 31 0 1 0 156
49 161 34 10.4 8.91 2 10.3 73 25 0 2 0 173
56 158 33 10.5 9.04 0 8.2 69 28 2 0 l 190
63 137 34 10.7 8.77 l 7.9 70 28 0 2 0 204
70 144 32 10.5 9.18 O 10.6 72 27 0 l O 216
77 124 34 10.0 9.25 1 8.4 68 31 0 1 0 225
84 133 31 9.8 8.79 2 9.3 66 33 0 l 0 233
91 123 33 10.5 8.69 2 8.1 69 30 0 l 0 240
S.I. = Serum Iron (micrograms/100 ml. blood)
Hc. = Hematocrit (percent)
Hb. = Hemoglobin (grams/100 ml. blood)
RBC = Red blood cell (105)
Ret. = Reticulocyte (number/500 erythrocytes)
WBC = White blood cell (103)
Differential
N a Neutrophil (%)
L = Lymphocyte (%)
M = Monocyte (%)
E a Eosinophil (%)
B = BasoPhil (%)
B.W. a Body weight (pounds)

38

 

 

TABLE 21. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 21
Age D e
(days) S.I. Hc. Hb. RBC Ret. WBC N L M E B B.W.
initial 108
7 75 31 9.8 10.49 0 12.3 72 28 0 0 0 115
14 131 32 10.2 9.71 37 9.0 59 41 0 0 0 125
21 166 36 10.6 9.97 9 8.7 75 25 0 0 0 142
28 183 37 9.6 9.87 28 8.9 56 44 0 0 0 153
35 174 41 9.8 10.40 31 14.0 64 36 0 0 0 167
42 165 42 10.0 10.27 2 13.3 61 39 0 0 0 184
49 183 40 10.8 10.33 0 9.6 57 43 0 0 0 204
56 165 36 11.2 9.53 0 8.9 53 47 0 0 0 222
63 179 37 11.6 9.60 0 10.6 51 48 0 l 0 238
70 163 36 11.0 10.12 5 7.8 49 51 0 0 0 250
77 156 36 11.2 10.49 0 11.2 60 40 0 0 0 263
84 160 36 11.2 9.96 0 9.3 56 43 O 1 0 275
91 170 35 11.0 10.23 3 9.6 62 37 0 1 0 287
TABLE 22. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 22
Age D n
(days) S.I. Hc. Hb. RBC Ret. WBC N L M E E B.W.
initial 96
7 104 28 7.2 7.02 5 8.4 61 39 0 0 0 99
14 94 27 6.8 6.94 0 10.1 70 30 0 0 O 105
21 102 28 6.0 6.85 1 7.4 57 43 0 0 0 113
28 96 28 6.4 6.87 0 8.3 68 32 0 0 0 122
35 101 28 6.6 7.00 l 9.6 58 42 0 0 0 132
42 93 28 6.6 7.12 4 7.9 67 33 0 0 0 141
49 82 27 6.8 7.19 0 11.4 59 41 0 0 0 152
56 96 28 6.4 7.27 l 9.6 64 34 0 2 0 163
63 100 28 6.4 7.20 0 10.8 59 40 1 0 0 172
70 96 28 6.2 7.31 4 6.5 70 29 0 1 0 183
77 93 27 6.2 7.33 l 8.4 62 38 0 0 0 191
84 101 27 6.2 7.36 0 9.3 57 43 0 0 0 205
91 89 27 5.8 7.40 1 10.9 60 39 l 0 0 217
S.I. = Serum Iron (micrograms/100 ml. blood)
Hc. = Hematocrit (percent)
Hb. a Hemoglobin (grams/100 ml. blood)
RBC a Red blood cell (105)
Ret. = Reticulocyte (number/500 erythrocytes)
WBC a White blood cell (103)
Differential
N = Neutrophil (%)
L = Lymphocyte (%)
M = Monocyte (%)
E = EosinOphil (%)
B = Basophil (%)
B.W. = Body weight (pounds)

39

 

 

TABLE 23. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO.
Age D r 1
(days) S.I. HC. Hb. RBC Ret. WBC N L M E B B.W.
initial 116
7 73 34 9.8 8.67 7 8.7 70 30 0 0 0 122
14 68 34 9.4 8.73 5 8.9 67 33 0 0 0 132
21 68 34 9.2 8.78 2 10.6 62 38 0 0 0 145
28 69 34 9.2 8.71 7 7.8 59 41 0 0 0 154
35 74 34 9.4 8.52 8 11.3 54 44 0 2 0 167
42 80 35 9.4 8.66 l 9.4 53 47 0 0 0 178
49 72 34 9.0 8.81 2 10.6 63 36 0 0 1 193
56 69 34 9.2 8.97 8 10.2 59 41 0 O 0 207
63 80 34 9.0 8.91 3 9.8 57 39 0 4 0 222
70 89 34 9.4 9.14 2 7.9 55 43 0 2 0 234
77 82 31 8.4 9.22 0 9.3 59 41 0 0 0 246
84 86 31 8.8 9.15 4 9.6 55 44 0 0 1 251
91 88 34 8.6 9.10 l 9.6 47 50 0 3 0 266
TABLE 24. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO.
Age D
(days) 3.1. He. Hb. RBC Ret. WEC N L M E E B.W.
initial 121
7 106 36 10.4 9.21 4 7.6 54 45 0 1 0 131
14 133 36 10.0 8.71 4 8.4 55 45 0 0 0 142
21 149 35 10.4 8.48 0 10.3 48 51 0 l 0 157
28 163 36 10.0 8.93 13 9.4 53 47 0 0 0 171
35 168 38 10.2 8.75 4 6.8 52 48 0 0 0 185
42 163 41 10.2 8.71 12 5.4 47 53 0 0 0 199
49 148 39 10.2 8.79 4 9.6 60 40 0 0 0 212
56 159 40 10.4 8.84 0 12.3 57 41 0 2 0 224
63 151 40 10.2 8.59 2 8.4 44 55 0 l 0 238
70 162 41 10.4 8.95 0 9.4 57 43 0 0 0 250
77 149 37 10.8 9.17 3 10.3 51 49 0 0 0 262
84 167 39 10.4 9.03 2 9.1 54 46 0 0 0 276
91 154 39 10.2 8.86 8 7.5 53 47 0 0 0 289
S.I. = Serum Iron (micrograms/100 ml. blood)
Hc. = Hematocrit (percent)
Hb. = Hemoglobin (grams/100 ml. blood)
RBC a Red blood cell (106 )
Ret. = Reticulocyte (number/500 erythrocytes)
WBC = White blood cell (103 )
Differential
N = Neutrophil (%)
L = Lymphocyte (%)
M a Monocyte (%)
E = Eosinophil (%)
B = Basophil (%)
B.W. = Body weight (pounds)

23

24

40

 

 

TABLE 25. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 25
Age W.
(days) S.I. Hc. Hb. REC Ret. WBC N L M E B B.W.
initial 99
7 79 32 7.2 8.15 1 12.4 62 38 0 0 O 103
14 148 31 7.0 8.03 24 14.2 57 43 0 0 0 114
21 187 33 6.8 7.85 18 16.8 59 37 l 2 l 128
28 188 34 7.0 8.00 30 15.4 72 28 0 0 0 143
35 193 36 7.0 7.87 8 13.6 59 41 0 0 0 158
42 210 37 7.2 7.53 0 14.7 60 37 0 3 0 171
49 206 35 7.2 7.02 1 10.3 58 39 l 0 2 186
56 211 37 7.6 7.26 2 12.1 71 29 0 0 0 204
63 214 35 7.4 7.92 0 11.7 58 42 0 0 0 220
70 213 37 7.6 6.95 2 9.8 60 37 0 3 0 235
77 201 36 7.0 7.48 0 10.6 56 44 0 0 0 248
84 257 33 7.2 7.81 4 11.4 59 39 l l 0 262
91 192 37 7.6 8.08 1 11.8 59 41 0 0 0 275
TABLE 26. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 26
Age W
(daY8-) S.I. HC. 1"le RBC Reta WBC N L M E B BeWe
initial 104
7 143 33 8.5 6.47 8 9.8 62 38 0 0 0 112
14 211 34 8.2 6.67 41 7.2 53 47 0 0 0 122
21 236 38 8.6 6.63 15 13.1 60 39 0 l O 136
28 249 39 8.3 6.55 19 12.6 60 40 0 O 0 150
35 254 40 8.4 6.30 6 9.8 56 44 0 0 0 165
42 240 39 8.4 6.73 4 11.1 58 41 0 1 0 181
49 256 37 8.8 6.44 2 11.0 57 43 O 0 0 196
56 260 38 9.1 6.64 3 12.1 60 40 0 O 0 215
63 268 33 8.8 7.19 2 9.3 63 37 0 0 0 230
70 249 37 9.2 6.38 0 9.4 57 43 0 O O 243
77 246 36 8.8 6.66 3 8.6 65 35 0 0 0 256
84 252 37 8.8 6.32 2 11.7 60 38 0 2 0 266
91 238 37 8.9 6.71 0 13.1 68 30 0 2 0 276
S.I. a Serum Iron (micrograms/100 ml. blood)
HC. 3 Hematocrit (percent)
Hb. = Hemoglobin (grams/£00 ml. blood)
RBC . Red blood cell (10 )
Ret. s Reticulocyte (number/500 erythrocytes)
WBC - White blood cell (103)
Differential
.N = NeutrOphil (%)
L - Lymphocyte (%)
M = Monocyte (%)
E a Eosinophil (%)
B = 3330531111 (%)

Body weight (pathos?

41

 

 

TABLE 27. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF N0.
Age D3§ferential
(days) S.I. HC. Hb. RBC Ret. WBC N L M E B B.W.
initial 99
7 54 44 13.0 10.13 5 8.6 59 41 0 0 0 112
14 72 41 12.8 9.90 4 14.1 52 47 0 1 0 125
21 106 43 13.4 10.02 14 11.8 71 29 0 0 0 137
28 104 44 13.2 10.06 5 8.0 80 20 0 0 0 150
35 99 47 13.6 9.51 21 7.9 61 39 0 0 0 163
42 118 48 13.2 9.76 1 9.4 52 47 0 l 0 174
49 101 47 13.0 9.64 10 8.6 56 44 0 0 0 187
56 109 47 13.0 9.80 9 10.2 63 36 0 1 0 198
63 103 46 12.6 9.93 7 12.3 59 41 0 0 0 213
70 117 47 13.0 9.71 15 11.4 66 33 1 0 0 226
77 105 48 13.4 9.69 7 8.6 56 43 0 l 0 239
84 112 48 13.6 H 9.67 5 10.1 58 42 0 0 0 250
91 108 46 13.2 9.58 10 11.4 60 40 0 0 0 264
TABLE 28. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO.
Age D f n a
(days) S.I. HC. Hb. REC Ret. WBC N L M E B B.W.
initial 118
7 138 34 9.8 7.97 O 12.3 69 31 0 0 0 115
14 160 32 9.6 8.05 4 14.2 67 33 0 0 0 130
21 174 33 10.0 8.13 6 16.6 62 38 0 0 0 143
28 176 34 10.4 8.37 0 15.5 63 37 0 0 0 158
35 171 36 10.2 8.21 7 14.7 56 44 0 0 0 170
42 169 38 10.2 8.34 3 17.3 56 42 l 0 2 179
49 191 37 10.2 8.39 6 14.8 54 46 0 0 0 189
56 186 38 10.4 8.52 13 11.7 58 41 0 0 1 201
63 182 38 10.4 8.60 5 12.4 55 43 0 0 2 212
70 189 39 10.0 8.16 2 10.8 56 44 0 0 0 220
77 180 38 10.0 8.07 9 13.2 59 37 0 1 3 229
84 192 38 9.6 8.12 3 11.9 71 28 0 0 1 240
91 175 38 8.6 8.13 7 12.6 66 31 0 2 1 249
S.I. = Serum Iron (micrograms/100 ml. blood)
Hc. = Hematocrit (percent)
Hb. a Hemoglobin (grams/100 ml. blood)
RBC = Red blood cell (105)
Ret. = Reticulocyte (number/500 erythrocytes)
WEC e White blood cell (103)
Differential
N = Neutrophil (%)
L = Lymphocyte (%)
M = Monocyte (%)
E = Eosinophil (%)
B = Basophil (%)
B.W. = Body weight (pounds)

27

28

42

 

 

TABLE 29. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 29
Age D e a
(days) 5.1. He. Hb. REC Ret. WBC N L M E E B.W.
initial 112
7 100 34 12.4 9.12 3 8.6 68 31 0 l 0 115
14 153 33 12.0 9.21 53 10.4 71 29 0 0 0 122
21 210 35 12.0 9.17 14 9.6 63 37 0 0 0 131
28 205 38 11.8 9.30 28 7.3 53 46 0 1 0 140
35 202 37 12.0 9.33 8 5.9 56 43 1 0 0 150
42 196 37 11.6 9.18 S 5.4 49 51 0 0 0 163
49 206 36 11.8 9.40 0 5.8 52 48 0 0 0 179
56 189 37 12.2 9.37 1 7.4 46 54 0 0 0 192
63 214 38 12.4 9.07 0 6.3 58 41 1 0 0 203
70 201 35 12.4 9.02 2 9.4 45 55 0 0 0 211
77 182 36 12.0 9.23 l 9.8 55 44 0 1 0 221
84 175 35 12.2 9.50 0 9.1 49 51 0 0 0 229
91 186 36 12.4 9.47 2 9.9 58 42 0 O O 239
TABLE 30. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 30
Age D
(days) S.I. HC. Hb. RBC Ret. WBC N L M E B B.W.
initial 113
7 76 34 12.7 9.76 11 8.7 60 40 0 0 0 117
14 118 34 12.5 9.90 34 9.8 61 38 0 1 0 127
21 179 38 12.8 10.10 25 12.1 56 41 1 2 0 139
28 182 37 12.9 9.94 39 7.6 46 52 0 2 0 151
35 178 38 12.8 9.89 12 11.4 57 42 0 l 0 165
42 175 37 13.0 9.37 0 8.6 43 55 0 2 O 179
49 180 38 12.6 8.92 0 9.3 53 47 0 0 0 195
56 179 38 13.1 9.30 2 10.4 43 54 0 3 0 213
63 180 38 13.2 9.50 2 8.7 56 43 0 1 0 228
70 172 38 13.0 9.90 2 9.6 41 58 O 1 0 238
77 160 39 13.0 9.71 0 9.4 49 50 1 0 0 250
84 173 38 12.8 9.79 2 10.0 41 57 0 0 0 260
91 174 38 13.5 10.07 1 11.4 49 51 0 0 O 267
S.I. = Serum Iron (micrograms/100 ml. blood)
Hc. = Hematocrit (percent)
Hb. a Hemoglobin (grams/100 ml. blood)
RBC - Red blood cell (105)
Ret. a Reticulocyte (number/500 erythrocytes)
WEC e White blood cell (103)
Differential
N a Neutrophil (%)
L = Lymphocyte (%)
M = Monocyte (%)
E a Eosinophil (%)
, . B e Basophil,(%) xnwm,q
B.W. a Body weight (p Ends)

43

 

 

32

TABLE 31. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 31
Age D
(days) S.I. Hc. Hb. REC Ret. WBC N L M E B B.W.
initial 123
7 81 37 10.8 9.12 2 7.8 56 44 0 0 0 130
14 70 37 10.6 9.18 12 12.1 41 57 l 0 1 139
21 63 36 10.2 9.21 0 9.4 39 61 0 0 0 149
28 72 36 9.6 9.25 1 10.3 53 47 0 0 0 156
35 64 35 10.4 9.11 0 9.5 36 63 0 0 l 165
42 80 38 9.8 9.33 5 9.4 51 49 0 0 0 176
49 76 36 9.6 9.37 0 7.8 40 58 2 0 0 190
56 71 36 9.4 9.45 0 9.6 45 54 0 1 0 203
63 61 36 9.6 9.37 0 8.6 42 57 0 l 0 214
70 79 35 9.6 9.47 2 9.6 45 55 0 0 0 223
77 66 36 9.6 9.42 2 8.3 40 60 0 0 0 231
84 72 36 9.6 9.40 2 8.9 44 56 0 0 0 240
91 68 38 9.0 9.53 2 7.9 38 62 0 0 0 251
TABLE 32. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO.
Age D
(days) S.I. Hc. Hb. RBC Ret. W8C N L M E B B.W.
initial 106
7 68 35 11.1 10.02 0 9.6 41 58 0 1 0 109
14 123 34 10.7 9.83 41 11.4 48 51 l 0 0 118
21 181 39 10.8 9.90 28 8.6 45 55 0 0 0 127
28 182 39 11.1 10.19 15 10.8 53 47 0 0 0 138
35 175 41 11.1 10.26 0 11.7 43 54 0 3 0 148
42 168 40 10.8 10.07 2 10.3 44 54 0 2 0 160
49 169 42 11.1 10.42 2 11.4 46 53 0 1 0 175
56 170 40 11.5 9.74 0 7.8 47 51 0 2 0 191
63 171 38 11.4 9.90 0 9.3 60 38 0 2 0 200
70 160 35 11.4 9.97 3 9.8 51 45 0 4 0 212
77 150 39 11.0 9.84 3 8.4 63 36 0 l 0 220
84 143 39 11.0 9.93 3 10.6 48 48 0 4 0 232
91 138 39 11.7 9.97 3 9.7 57 41 0 2 0 241
S.I. = Serum Iron (micrograms/100 ml. blood)
Hc. = Hematocrit (percent)
Hb. . Hemoglobin (grams/100 ml. blood)
RBC = Red blood cell (105)
Ret. = Reticulocyte (numberéSOO erythrocytes)
WBC - White blood cell (10 )
Differential
N = NeutrOphil (%)
L a Lymphocyte (%)
M = Monocyte (%)
E = Eosinophil (%)
B = Basophil (%)
B.W. a Body weight (pounds)

44

 

 

TABLE 33. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 33
Age
(days) S.I. Hc. Hb. REC Ret. W8C N L M E B B.W.
initial 86
7 85 24 7.2 5.97 2 11.6 50 49 0 l 0 90
14 80 24 6.8 6.09 3 9.8 51 48 0 1 0 97
21 82 25 6.6 6.20 5 12.0 41 58 0 1 0 106
28 86 26 5.8 5.76 2 13.1 56 44 0 0 0 115
35 82 25 6.4 5.52 2 11.5 60 40 0 0 0 125
42 83 26 6.0 5.38 4 8.3 58 42 0 O 0 136
49 88 25 6.0 5.42 0 7.6 49 50 0 1 0 149
56 88 25 5.8 5.48 0 10.4 46 54 0 O 0 164
63 81 25 6.0 5.60 5 11.1 46 53 1 0 0 176
70 90 25 6.0 5.82 1 7.5 42 56 0 2 O 187
77 85 25 6.4 5.53 -0 11.6 49 49 0 2 0 196
84 86 26 5.8 5.72 0 10.1 53 47 0 0 0 206
91 80 26 5.6 5.84 0 11.7 50 48 1 1 0 215
TABLE 34. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 34
Age
(days) S.I. HC. Hb. RBC Ret. WBC N L M E B B.W.
initial .. . . 79
7 90 32 8.2 6.38 12 7.6 46 54 0 0 O 81
14 118 30 8.0 6.55 4 12.7 55 45 0 0 0 93
21 151 32 8.5 6.48 2 13.8 47 52 0 l O 103
28 136 34 9.0 6.23 8 13.3 54 44 2 0 0 115
35 138 35 9.4 6.19 5 12.4 47 50 0 3 0 124
42 127 35 9.4 6.52 0 11.6 52 48 0 0 0 135
49 133 37 9.2 6.42 8 9.5 43 57 0 0 0 143
56 144 36 8.6 6.57 4 10.7 48 51 0 0 1 154
63 147 36 8.8 6.69 3 14.0 40 58 0 2 0 165
70 148 37 9.2 7.02 10 12.8 46 54 0 0 0 174
77 141 33 9.0 6.47 12 16.7 49 49 0 2 0 182
84 148 36 8.4 6.21 0 9.8 44 55 0 1 0 191
91 149 36 8.2 6.22 6 10.4 39 56 1 3 l 201
S.I. - Serum Iron (micrograms/100 ml. blood)
Hc. - Hematocrit (percent)
Hb. a Hemoglobin (grams/100 ml. blood)
REC - Red blood cell (105)
Ret. a Reticulocyte (number/500 erythrocytes)
WEC . White blood cell (103)
Differential
N - Neutrophil (%)
L = Lymphocyte (%)
M . Monocyte (%)
E . Eosinophil (%)
. y B a Basophil (%)
B.W. - Body weight (penndé)

45

 

 

TABLE 35. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 35
Age W.
(daYS) S.I. HC. Hb. RBC Reta WBC N L M E B B.W.
initial 95

7 53 23 7.6 6.77 10 7.3 61 39 0 0 0 104
14 79 23 7.8 6.72 4 5.3 57 43 0 0 0 113
21 118 23 8.0 6.76 9 5.9 55 44 0 1 0 127
28 110 25 8.4 6.59 11 6.7 53 47 0 0 0 141
35 98 27 8.4 6.81 8 5.4 49 51 0 0 0 153
42 106 27 8.0 6.69 16 7.3 44 55 0 0 1 168
49 104 27 8.4 6.74 9 8.4 53 47 0 0 0 183
56 103 26 8.6 6.78 6 6.5 46 53 0 1 0 196
63 117 26 8.6 6.51 8 5.3 47 49 0 4 0 208
70 109 28 8.8 6.57 5 8.0 52 48 0 0 0 221
77 109 25 8.2 6.68 12 9.1 45 54 0 0 1 235
84 106 26 8.8 6.60 11 7.4 57 41 0 2 0 249
91 110 27 9.4 6.67 3 7.6 58 39 0 3 0 263
TABLE 36. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 36
Age W
(days) S.I. Hc. Hb. REC Ret. WBC N L M E B B.W.
initial 93
7 151 36 11.4 7.77 2 12.5 60 39 0 l 0 97

14 190 37 11.6 8.11 32 16.2 55 44 0 1 0 105
21 256 38 11.0 8.00 16 15.0 52 47 0 0 1' 115

l 28 253 40 11.6 7.91 12 14.3 57 43 0 0 0 124
35 239 38 11.8 8.12 4 9.8 58 42 0 0 0 133
42 230 36 10.8 7.67 3 12.3 53 45 0 2 0 145
49 228 40 11.6 7.91 3 11.5 61 39 0 0 0 155
56 261 39 12.2 7.93 3 14.2 50 46 1 2 1 169
63 264 35 12.0 8.05 2 10.6 62 38 0 0 0 182
70 259 37 11.4 7.65 0 9.8 55 41 l 3 0 193
77 257 39 11.0 7.83 2 10.4 57 40 O 2 1 203
84 246 38 11.6 8.56 0 11.7 52 47 0 1 0 211
91 240 38 11.9 8.05 0 12.1 51 45 0 4 O 222
S.I. = Serum Iron (micrograms/100 ml. blood)

Hc. - Hematocrit (percent)

Hb. a Hemoglobin (grams/100 ml. blood)

RBC = Red blood cell (106 )

Ret. - Reticulocyte (number/500 erythrocytes)

WBC a White blood cell (103 )

Differential

N - Neutrophil (%)

= Lymphocyte (%)
Monocyte (%)
Eosinophil (%)
Basophil (%)

Body weight (pounds)

L
M
E
B

46

TABLE 37. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 37

Age W.
(days) S.I. Hc. Hb. RBC Ret. WBC N L M E B B.W.
initial 93
7 57 30 9.8 6.80 0 11.7 60 40 0 0 0 95
14 60 30 10.0 6.87 0 12.4 61 38 0 0 1 101
21 54 30 8.6 6.79 0 8.3 53 46 0 l 0 110
28 59 29 8.0 6.88 1 6.5 63 37 0 0 0 119
35 62 28 8.8 6.95 1 9.4 56 43 0 0 1 130
42 63 29 8.2 6.98 O 10.2 62 38 0 0 0 145
49 68 29 8.4 6.85 7 9.6 48 52 0 0 0 163
56 59 32 8.0 6.87 4 7.8 57 43 0 0 0 182
63 64 31 8.0 7.02 0 11.3 50 49 l 0 0 188
70 70 32 8.4 6.98 3 9.4 48 51 l 0 0 196
77 66 31 7.8 7.04 3 9.6 49 50 0 0 l 204
84 63 31 8.2 7.10 2 10.4 46 54 0 0 0 210
91 58 32 8.0 7.14 4 11.1 47 53 0 0 0 217

 

TABLE 38. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 38

 

Age W
(days) S.I. Hc. Hb. REC Ret. WBC N L M E E B.W.
initial 91
7 137 26 8.3 6.94 3 8.8 52 48 0 0 0 98
14 164 26 9.0 6.84 1 9.6 53 46 0 1 0 112
21 183 27 8.4 6.79 1 12.1 54 46 0 O 0 125
28 197 28 9.3 6.72 5 9.3 51 48 0 l 0 140
35 181 30 9.2 6.64 4 8.7 55 45 0 O 0 155
42 190 31 9.5 6.78 0 9.6 56 44 0 0 0 167
49 194 30 9.8 6.81 7 10.0 60 39 l 0 0 183
56 200 30 9.1 7.05 8 11.3 61 37 O 2 0 198
63 206 31 9.3 6.99 l 8.6 58 41 l 0 0 211
70 196 31 9.0 6.77 0 9.7 46 52 1 0 l 224
77 203 28 9.6 6.53 8 8.3 46 S4 0 0 0 238
84 201 31 9.6 6.57 3 9.2 41 58 0 0 1 251
91 210 31 10.5 6.61 8 10.1 50 49 0 0 1 265
S.I. 3 Serum Iron (micrograms/100 ml. blood)
HC. - Hematocrit (percent)
Hb. a Hemoglobin (grams/100 ml. blood)
REC a Red blood cell (106)
Ret. a Reticulocyte (number/500 erythrocytes)
WBC e White blood cell (103)
Differential
N = Neutrophil (%)
L = Lymphocyte (%)
M a Monocyte (%)
E a Eosinophil (%)
B = Basophil (%)
B.W. a Body weight (pounds)

47

 

 

TABLE 39. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 39
Age D
(days) S.I. Hc. Hb. RBC Ret. WBC N L M E B B.W.
initial 80
7 115 37 10.1 8.53 0 4.7 72 28 0 0 0 84
14 160 36 10.2 8.49 60 6.3 61 39 0 0 0 94
21 208 39 9.8 8.75 31 5.1 71 29 0 0 0 106
28 210 41 9.7 8.62 40 8.4 64 34 0 2 0 118
35 203 43 9.5 8.24 7 9.3 63 37 0 O 0 130
42 211 40 10.2 8.36 2 7.8 68 28 0 4 0 145
49 205 42 10.6 8.69 0 8.4 60 40 0 0 0 162
56 206 40 11.1 8.75 l 9.6 54 43 0 3 0 178
63 197 38 11.4 8.52 4 10.1 44 48 0 6 0 193
70 188 39 10.7 8.56 2 7.9 67 33 0 0 0 206
77 181 38 10.5 8.62 0 8.3 59 40 0 1 0 218
84 175 38 10.7 8.75 1 9.4 69 29 1 1 0 229
91 172 39 10.7 8.78 3 10.4 69 31 0 0 0 240
TABLE 40. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 40
Age WI.
(days) S.I. Hc. Hb. REC Ret. WBC N L M E E B.W.
initial 69
7 170 38 11.7 8.56 3 9.1 59 41 0 0 O 75
14 203 38 11.4 8.70 27 9.4 59 4070 1 0 83
21 258 37 11.8 8.62 28 8.5 52 48 0 0 0 90
28 242 41 11.3 8.81 34 8.9 49 50 0 O 1 100
35 246 42 11.2 8.73 2 7.6 47 43 O 0 0 112
42 256 41 11.2 8.31 1 9.0 46 54 0 0 0 123
49 251 41 11.6 8.52 6 10.3 51 49 0 0 0 135
56 243 42 11.9 9.39 0 11.4 43 56 0 l 0 149
63 271 43 12.0 8.24 0 9.8 52 48 0 0 0 162
70 259 41 11.4 8.39 1 11.9 49 49 1 1 0 171
77 259 41 12.0 8.73 2 12.6 58 40 1 1 0 180
84 246 38 11.6 8.82 2 9.8 60 40 0 0 0 188
91 242 38 11.7 9.07 1 10.3 57 42 1 0 0 198
3.1. a Serum Iron (micrograms/100 ml. blood)
RC. = Hematocrit (percent)
Hb. = Hemoglobin (grams/100 ml. blood)
RBC e Red blood cell (106)
Ret. = Reticulocyte (numberfiSOO erythrocytes)
WBC a White blood cell (10 )
Differential
N = Neutrophil (%)
L = Lymphocyte (%)
M = Monocyte (%)
E = Eosinophil (%)
B = BaSOphil (%)
B.W. = Body weight (pounds)

48

 

 

TABLE 41. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 41
Age D;f§g;eg;;§1
(days) S.I. HC. Hb. RBC Ret. WBC N L M E B B.W.
initial 86
7 66 41 13.4 8.38 3 9.9 61 39 0 0 0 93
14 65 41 12.5 8.24 0 13.2 56 44 0 0 0 100
21 59 41 11.8 8.30 0 11.5 59 41 0 0 0 111
28 70 38 11.4 8.41 0 14.3 46 53 0 1 0 120
35 68 38 11.8 8.51 8 10.8 58 47 0 0 0 131
42 70 38 11.2 8.60 2 9.6 44 55 0 1 0 143
49 70 40 11.4 8.66 0 12.4 52 48 0 0 0 159
56 63 42 11.1 8.55 4 11.0 49 51 0 0 O 178
63 68 43 11.6 8.59 0 8.8 56 44 0 0 0 192
70 61 42 11.0 8.69 l 9.7 52 47 0 1 0 202
77 64 41 11.4 8.96 0 10.4 55 43 0 2 0 214
84 71 40 11.4 8.70 2 11.1 60 40 0 0 0 222
91 66 41 11.0 8.77 0 12.6 58 42 0 0 0 234
TABLE 42. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 42
Age _llliieaeeilel.
(days) S.I. HC. Hb. RBC Ret. WBC N L M E B B.W.
initial 98
7 81 40 10.4 8.23 4 12.4 62 37 0 0 1 103
14 119 38 10.6 8.32 39 7.6 61 39 0 0 0 111
21 173 45 10.0 8.03 22 8.4 61 39 0 0 0 121
28 178 44 10.8 8.73 44 9.6 58 40 1 0 1 130
35 190 46 11.0 7.97 10 10.3 72 28 0 0 0 139
42 181 44 10.5 8.10 0 8.7 53 45 0 2 0 152
49 190 44 10.8 8.15 3 6.8 55 41 0 3 l 164
56 183 46 10.8 8.37 3 7.4 50 46 0 4 0 175
63 184 43 10.8 8.58 2 8.4 56 43 1 0 0 186
70 189 44 10.0 8.39 4 9.3 50 48 0 2 0 195
77 178 44 11.0 8.53 3 10.1 55 45 0 0 0 205
84 180 43 10.6 7.63 l 7.9 55 40 0 5 0 216
91 183 43 10.2 8.08 2 8.8 55 44 l 0 0 228
S.I. - Serum Iron (micrograms/100 ml. blood)
Me. a Hematocrit (percent)
Hb. = Hemoglobin (grams/100 ml. blood)
RBC = Red blood cell (106 )
Ret. a Reticulocyte (number/500 erythrocytes)
WBC = White blood cell (103 )
Differential
N = Neutrophil (%)
L = Lymphocyte (%)
M = Monocyte (%)
E = Eosinophil (%)
B = Basophil (%)
B.W. = Body weight (pounds)

49

 

 

TABLE 43. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 43
Age D;§fie;§n§i§1
(days) S.I. Hc. Hb. RBC Ret. WBC N L M E B B.W.
initial 101
7 101 42 11.1 8.62 6 12.5 60 39 0 l 0 106
14 148 42 10.2 8.51 36 9.3 59 41 0 0 0 114
21 187 44 11.0 8.53 15 10.6 60 40 0 0 0 125
28 192 47 10.8 8.42 17 14.1 49 50 0 l O 134
35 208 47 10.6 8.38 0 11.2 42 54 0 4 0 145
42 193 47 10.8 8.30 2 7.5 43 56 l 0 0 159
49 199 46 11.4 8.04 2 9.0 49 49 0 2 0 175
56 209 47 11.7 8.63 0 9.8 47 53 0 0 0 189
63 206 47 11.6 8.32 0 9.4 42 55 0 3 0 201
70 195 43 11.0 7.28 0 10.1 41 58 0 1 0 213
77 186 44 11.7 8.78 2 9.8 44 54 0 2 0 221
84 196 43 11.8 8.51 3 7.8 44 55 1 0 0 233
91 208 43 11.5 8.24 0 11.1 49 49 0 1 l 242
TABLE 44. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 44
Age D
(days) S.I. HC. Hb. RBC Ret. WBC N L M E B B.W.
initial 85
7 136 33 10.8 8.19 l 8.5 49 51 0 0 0 91
14 130 33 10.4 8.75 0 5.3 52 48 0 0 O 99
21 133 33 9.6 8.39 0 4.6 53 47 0 0 0 110
28 130 33 9.2 8.32 0 7.3 54 46 0 0 0 121
35 123 33 9.6 8.30 0 9.6 61 39 0 0 0 132
42 128 35 9.4 8.44 5 12.9 53 47 0 0 0 144
49 138 33 9.4 8.38 9 10.3 58 41 0 l 0 158
56 141 34 9.6 8.51 1 12.1 60 40 0 0 0 172
63 139 34 9.6 8.37 0 9.1 61 38 0 1 0 185
70 136 33 9.2 8.53 4 10.0 60 36 0 4 0 197
77 140 35 9.4 8.81 0 7.9 65 35 0 0 0 211
84 145 34 9.0 8.65 0 8.7 60 39 0 0 1 219
91 138 35 8.4 8.34 5 8.1 57 43 0 0 0 231
S.I. - Serum Iron (micrograms/100 ml. blood)
Hc. = Hematocrit (percent)
Hb. = Hemoglobin (grams/100 ml. blood)
RBC a Red blood cell (106 )
Ret. = Reticulocyte (number/500 erythrocytes)
WBC = White blood cell (103 )
Differential
N a Neutrophil (%)
L a Lymphocyte (%)
M = Monocyte (%)
E = Eosinophil (%)
B a Basophil (%)
B.W. = Body weight (pounds)

50

TABLE 45. HEMATOLOGICAL FINDINGS AND BODY WEIGHTS, CALF NO. 45

 

Age W131.
(days) S.I. Hc. Hb. RBC Ret. W8C N L M E B B.W.
initial 108
7 115 43 13.2 9.19 2 9.5 53 47 0 0 0 114
14 109 43 12.6 9.15 11 7.3 54 46 0 0 0 121
21 101 43 12.0 9.25 2 6.8 51 49 0 0 0 133
28 110 41 11.2 9.32 0 7.6 46 53 0 1 0 142
35 111 40 11.4 9.37 0 8.3 49 51 0 0 0 154
42 108 42 11.2 9.23 0 9.0 52 47 0 0 1 163
49 106 44 10.8 9.51 1 7.8 50 48 0 2 0 174
56 121 43 11.6 9.48 1 11.5 57 43 O 0 0 186
63 114 45 11.8 9.96 0 9.6 64 34 0 2 0 197
70 128 42 12.2 9.42 l 7.9 58 41 0 1 0 207
77 118 43 11.6 9.80 0 8.4 67 30 0 2 1 216
84 125 43 11.5 9.63 2 8.8 59 41 0 0 0 225
91 112 42 11.2 9.87 5 9.4 55 44 0 1 0 238
S.I. - Serum Iron (micrograms/100 ml. blood)
HC. . Hematocrit (percent)
Hb. a Hemoglobin (grams/100 ml. blood)
RBC . Red blood cell (106)
Ret. - Reticulocyte (number/500 erythrocytes)
WBC a White blood cell (103)
Differential
N = Neutrophil (%)
L = Lymphocyte (%)
M a Monocyte (%)
E a Eosinophil (%)
B a Basophil (%)
B.W. a Body weight (pounds)

BIBLIOGRAPHY

Balfour, W. M., Hahn, P. F., Bale, W. F., Pommerenke, W. T.,
and Whipple, G. H.: Radioactive Iron Absorption
in Clinical Conditions: Normal, Pregnancy, Anemia,
and Hemochromatosis. Jour. of Exper. Med., 76 (1942):
15-30.

Benjamin, M. M.: Outline of Veterinary Clinical Pathology,
2nd ed. The Iowa State University Press, Ames, Iowa,
1961.

Bernhart, F. W., and Skeggs, L.: The Iron Content of Crystal-
line Human Hemoglobin. Jour. of Biol. Chem., 147
(1943): 19-22.

Bessis, M. C., and Breton-Gorius, J.: Ferritin and Ferrugi-
nous Micelles in Normal Erythroblasts and Hypochromic
Hypersideremic Anemias. Blood, 14 (1959): 423-432.

Blaxter, K. L., Sharman, G. A. M., and MacDonald, A. M.:
Iron-deficiency Anemia in Calves. British Jour. of
Nutri., 11 (1957): 234-246.

Brown, E. B., Jr., Dubach, R., and Moore, C. V.: Studies
in Iron Transportation and Metabolism. Critical
Analysis of Mucosal Block by Large Doses of Inor-
ganic Iron in Human Subjects. .Jour. of Lab. and
Clin. Med., 52 (1958): 335-355.

Brown, E. B., and Justus, B. W.: In Vitro Absorption of
Radioiron by Everted Pouches of Rat Intestine. Am.
Jour. of Phys., 194 (1958): 319-326.

Byers, J. H., Jones, I. R., and Haag, J. R.: Blood Hemoglo-
bin Values of Dairy Cattle. Jour. of Dairy Sci.,
36 (1952): 661-667. -

Carlson, R. H., Swenson, M. J., Ward, G. M., Booth, N. H.:
Effect of Intramuscular Injections of Iron-Dextran
in Newborn Lambs and Calves. Jour. of the Am. Vet.
Ned. ASSOCO’ 139 (1961): 457-461.

Cartwright, G. E., Gubler, C. J., Bush, J. A., and Wintrobe,
M. M.: Studies on Copper Metabolism. Further Ob-
servations on the Anemia of Copper Deficiency in
Swine. Blood, 11 (1956): 143-153.

51

52

Chase, M. S., Gubler, C. J., Cartwright, G. E., and Wintrobe,
M. M.: Influence of Copper on Iron Absorption.
Fed. Pro., 11 (1952): 438.

Copp, D. H., and Greenberg, D. M.: A Tracer Study of Iron
Metabolism with Radioactive Iron. 1. Methods:
Absorption and Excretion of Iron. Jour. Biol. Chem.
164 (1946): 377-387.

Coryell, D. C., and Pauling, L.: A Structural Interpreta-
tion of the Acidity of Groups Associated with the
Memes of Hemoglobin and Hemoglobin Derivatives.
Jour. of Biol. Chem., 132 (1940): 769-779.

Drabkin, D. L., Metabolism of the Hemin Chromoproteins.
Physiological Reviews, 31 (1951): 345-431.

Elvehjem, C. A., and Sherman, W. C.: The Action of Copper
in Iron Metabolism. Jour. Biol. Chem., 98 (1932):
309-319.

Finch, S. C., and Finch, C. A.: Idiopathic Hemochromatosis,
An Iron Storage Disease. A. Iron Metabolism in
Hemochromatosis. Medicine, 34 (1955): 381-386.

Follis, R. H., Jr.: Deficiency Disease. Charles C. Thomas,
Springfield, Illinois, 1958.

Foster, P. C.: The Effects of Radiant Energy on Milk Anemia
in Rats. Jour. of Nutri., 4 (1931): 517-524.

Fowler, W. M.: Chlorosis--An Obituary. Annals of Medical
History, 8 (1936): 168-177.

Greenberg, D. M., Copp, D. H., and Cuthbertson, E. M.:
Studies in Mineral Metabolism with the Aid of Arti-
ficial Radioactive Isotopes. VII. The Distribution
and Excretion, Particularly by Way of the Bile, of
Iron, Cobalt, and Manganese. Jour. of Biol. Chem.,
147 (1943): 749-756.

Granick, 3.: Structure and Physiological Functions of Fer-
ritin. Physiologic Reviews, 31 (1951): 489-511.

Hahn, P. F.: Metabolism of Iron. Federation Proceedings,
7 (1948): 493-498.

Hahn, P. F.: The Metabolism of Iron. Medicine, 16 (1937):
249-266.

53

Hahn, P. F., Bale, W. F., Ross, J. F., Balfour, W. M., and
Whipple, G. H.: Radioactive Iron Absorption by Gas-
tro-Intestinal Tract. Influence of Anemia, Andxia,
and Antecedent Feeding Distribution in Growing Dogs.
Jour. of Exp. Med., 78 (1943): 169-188.

Heath, C. W., and Patek, A. J.: The Anemia of Iron Defici-
ency. Medicine, 16 (1937): 267-350.

Hegsted, D. M., Finch, C. A., and Kinney, T. D.: Influence
of Diet on Iron Absorption. III. Comparative Stud-
ies with Rats, Mice, Guinea Pigs, and Chickens.
Jour. of Exp. Med., 96 (1952): 115-119.

Houk, E. H., Thomas, A. W., and Sherman, H. C.: Some Inter-
relationships of Dietary Iron, Copper and Cobalt
in Metabolism. Jour. of Nutri., 31 (1946): 609-
620.

Hubbert, F., Jr., and Wallace, J. D.: The Effect of Inject-
able Iron and Oral Oxytetracycline on Hemoglobin,
Packed Cell Volume and Rate of Gain of Baby Range
Calves. Jour. of Animal Science, 18 (1959): 1180.

Iodice, A. A., Richert, D. A., and Schulman, M. P.: Copper
Content of Purified -Amino-levulinic Acid Dehydrase.
Fed. Pro., 17 (1958): 248.

Jensen, W. N., Bush, J. A., Ashenbrucker, H., Cartwright,
G. E., and Wintrobe, M. M.: The Kinetics of Iron
Metabolism in Normal Growing Swine. Jour. of Ex-
per. Med., 103 (1956): 145-159.

Johnston, F. A., McMillan, T. J., and Evans, E. R.: Per-
spiration as a Factor Influencing the Requirement
for Calcium and Iron. Jour. of Nutri., 42 (1950):
285-295.

Knoop, C. E., Krauss, W. B., and Washburn, R. G.: The De-
velopment of Nutritional Anemia in Dairy Calves.
Jour. of Dairy Sci., 18 (1935): 337-347.

Marston, H. R., and Lee, H. L.: Effects of Copper Defici-
ency and of Chronic Overdosage with Copper on Bor-
der-Leicester and Merino Sheep. Jour. of Agric.
Sci., 38 (1948): 229-240.

Matrone, G., Conley, C., Wise, G. H., and Waugh, R. K.:
A Study of Iron and Copper Requirements of Dairy
Calves. Jour. of Dairy Sci., 40 (1957): 1437-1447.

54

Moore, C. V.: Iron Metabolism and Nutrition. The Harvey
Lecture Series, 55 (1959-1960): 67-101.

Moore, C. V., Dubach, R., Minnich, V., and Roberts, H. K.:
Absorption of Ferrous and Ferric Radioactive Iron
by Human Subjects and by Dogs. Jour. of Clin. In-
vest., 23 (1944): 755-767.

Pirzio-Biroli, G., Bothwell, T. H., and Finch, C. A.: Iron
Absorption. II. The Absorption of Radioiron Admin-
istered with a Standard Meal in Man. Jour. of Lab.
and Clin. Med., 51 (1958): 37-48.

Planas, J., and de Castro, 3.: Serum Iron and Total Iron-
binding Capacity in Certain Mammals. Nature, 187
(1960): 1126-1127.

Raleigh, R. J., and Wallace, J. D.: The Influence of Iron
and Copper on Hematologic Values and on Body Weight
of Range Calves. Am. Jour. of Vet. Res., 23 (1962):
296-299.

Rimington, C.: Biosynthesis of Haemoglobin. British Med-
ical Bulletin, Series 15 (1959): 19-26.

Schade, A. L., and Caroline, L.: An Iron-binding Component
in Human Blood Plasma. Science, 104 (1946): 340-
341.

Schade, A. L., Reinhart, R. W., and Levy, H.: Carbon Diox-
ide and Oxygen in Complex Formation with Iron and
Siderophilin, the Iron-binding Component of Human
Plasma. Archives of Biochemistry, 20 (1949): 170-
172.

Schultze, M. 0.: The Relation of Copper to Cytochrome oxi-
dase and Hematopoietic Activity of the Bone Marrow
of Rats. Jour. of Biol. Chem., 138 (1941): 219-
224.

Smith, S. E., and Medlicott, M.: The Blood Picture of Iron
and Copper Deficiency Anemias in the Rat. Amer.
Joure PhYSie, 141 (1944): 354-358.

Smith, S. E., Medlicott, M., and Ellis, G. H.: The Blood
Picture of Iron and Copper Deficiency Anemias in
the Rabbit. Amer. Jour. of Physi., 142 (1944):

Sharpe, L. M., Peacock, W. C., Cooke, R., and Harris, R. S.:
The Effect of Phytate and Other Food Factors on Iron
Absorption. Jour. of Nutri., 41 (1950): 433-446.

55

Swenson, M. J., Underbjerg, G. K. L., Bartley, E. B., and
Jones, W. 6.: Effects of Trace Minerals, Aureomy-
cin, and Other Supplements on Certain Hematologic
Values and Organ Weights of Dairy Calves. Jour.
of Dairy Sci., 40 (1957): 1525-1533.

Thomas, J. W., Okamoto, M., Jacobson, W. C., and Moore, L. A.:
A Study of Hemoglobin Levels in the Blood of Young
Dairy Calves and the Alleviation of Anemia by Iron.
Jour. of Dairy Sci., 37 (1954): 805—812.

Tint, H., and Reiss, W.: Studies on the Purity and Specifi-
city of Cytochrome. II. Spectrophotometric Constants
and Molecular Weight Determination from the Content
of Iron. Jour. of Biol. Chem., 182 (1950): 397-403.

Ullrey, D. E., Miller, E. R., West, D. R., Schmidt, D. A.,
Seerley, R. W., Hoefer, J. A., and Luecke, R. W.:
Oral and Parenteral Administration of Iron in the
Prevention and Treatment of Baby Pig Anemia. Jour.
of Anim. Sci., 18 (1959): 256-263.

Underwood, E. J.: Trace Elements in Human and Animal Nutri-
tion. Academic Press, New York and London, 1962.

Vannotti, A., and Delachaux, A.: Iron Metabolism and its
Clinical Significance. Frederick Muller, Ltd., 29
Great James Street, W. C. 1, London, England, 1949.

Varley, H.: Practical Clinical Biochemistry, 3rd Ed., In-
terscience Publishers, Inc., New York, 1962.

Widdowson, E. M., and McCance, R. A.: The Absorption and
Excretion of Iron Before, During and After a Period
of Very High Intake. Biochem. Jour., 35, Part 2
(1937): 2029-2034.

Wilson, M. L., Iodice, A. A., Schulman, M. P., and Richert,
D. A.: Studies on Liver -Aminolevulinic Acid De-
hydrase. Fed. Pro., 18 (1959): 352-Article 1396.

Wohl and Goodhart: Modern Nutrition in Health and Disease.
Lea and Febiger, Philadelphia, 1960.

 

 

 

   

 

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