THESIS I ._..___-_ 7-, ' 1.; ‘ ..._._.-...__ This is to certify that the thesis entitled .Ln experimental analysis of abnormal fixation in the rat presented by Mr. Loo Ia Gladin has been accepted towards fulfillment of the requirements for I. A. degree mm Maj! professor 2 DateMZ/l/ffj / / / on "M AN EXPERIRENTAL ANALYSIS OF ABNOHhAL FIXATIUN In THE HAT By Leo L. Gladin A THESIS Submitted to the School of Graduate Studies of Hichigan State College of Agriculture and Applied Science in partial fulfillment of the requirements for the degree of MASTER OF ARTS Department of Psychology \ 1953 THESIS The author wishes to express his sincere thanks to Dr. M. Ray Denny, under whose direction the present investigation was undertaken. His assistance and guidance throughout the experiment and thesis preparation has been deeply appreciated. - .~\ ”'x *1 N'- 0 t LB‘iclkitj‘E List of Tables TABLE OF CONTENTS List of Figures I. II. III. IV. VI. VII. VIII. Introduction Statement of the Problem Methods and Procedure A. Apparatus B. Subjects C. Procedure Presentation of Results Discussion of Results Summary Appendix Bibliography Page ii 11 15 15 19 25 33 35 TABLE I. TABLE II. TABLE III. TABLE IV. LIST OF TABLES MEDIAN LATENCY BEFORE FALL MINUS MEDIAN LATENOY AFTER FALL ON DAY ONE AND DAY EIGHT OF TPE INSOLU- BLE PROBLEM. COMPARISON OF LEAN LATENCIES OF TI-E EIGHT! NON-LEARNING RATS ON TWO OR THREE SUCCESSIVE PRESEN- TATIONS OF THE NEGATIVE CARD ON THE)HABITUAL SIDE. (SOLUBLE PROB- LEM . 7 COMPARISON OF MEAN LATENCIES OF THE EIGHT NON-LEARE‘IING RATS ON PRESENTATION OF THE POSITIVE CARD ON THE HABITUAL SIDE AFTER ENTER AND AFTER FALL. (SOLUBLE PROBLEM). COMPARISON OF MEAN LATENC IES OF THE EIGHT NON-LEARNING RATS ON' TWO OR THREE SUCCESSIVE PRESENi- TATIONS OF THE POSITIVE CAM) ON THE)HABITUAL SIDE. (SOLUBLE PROB- IEL‘I o 55 35 36 36 LIST OF FIGURES Figure 1.: Ideal expectancy pattern illustrating variation in latency times after entry and after falling to the net. Figure 2.: Illustrating certain typical sequential probabilities of entry and reward dur- ing the insoluble problem.. Figure 3.: Illustrating.the sequential probabilities of entry and reward after a fall to the net during the soluble problem, for a rat fixated on the right. Figures 4,,5.: Atypical response pattern of one of the fixated rats.. Figure 6.: Typical orientation response toward the experimenter. Figures 7 through 15.: A generalization procedure used in break- ing fixation. ii 37 38 39 40 4o 41 INTRODUCTION In a series of experiments using the Lashley jumping apparatus, N. . F. Maier and his students (11) have succeeded in producing in rats a response pattern of such persistence as to warrant the designation fixation. While stereotypy of response has been reported by many other investigators, (h, S, 6) it is Meier's contention that the rigid behavior pattern found in rats subjected to his experimental designs is qualitatively different from that ordinarily subsumed under the postulates of learning theory. Further, he has held that the behavior patterns he has described should be considered as abnormal. fixations which result from the Operation of a dynamic mechanism of frustration and lead to behavior without a goal. The essential feature of the experimental design held responsible for "abnormal fixation" is the insoluble problem; the Lashley jumping apparatus was originally employed in visual discrimination problems, in which it was observed that some rats failed to make the required discrimination, but fixated a position response instead. It appeared to Maier that the jumping apparatus could possibly be used to create neuroses in rats in a discrimination problem setting (I). The general procedure employed in producing abnormal fixa- tions is as follows: The rat is trained to jump‘from a small stand to one of two windows which give access to a platform upon which food is diSplayed. When the rat learns to jump a distance of about eight inches, it is trained to knock down a card placed in a window. The cards which cover the window are readily discriminable, one being black with a small white circle in the center, the other being white with a small black circle in the center. The rat is given experience in jumping to both windows during the initial training period, care being taken to prevent the development of position habits or selec- tive preferences of one card over the other. After this training, during which both windows are unlocked and give access to food on each jump, the situation is altered so that both windows are randomly locked fifty percent of the time. No matter which card is selected or which position is assumed, the rat encounters a locked window on five of the ten daily trials. The rat usually refuses to jump after a few trials, and is then forced (goaded) to jump by means of electric shock, taps on the tail with a small stick, or 'by directing a jet of air under pressure at its posterior. Any attempts to jump in a direction other than the windows (abortive jumping) is discouraged. After a brief period of variability, the rat begins to express a consistent pattern of reSponse, usually in the form of a position stereotype: the rat jumps to either the left window or the right without further variation. This phase of the experiment is carried on for from eighty to ZhO trials. The problem is then "made soluble": one of the cards is con- sistently left unlocked, the other is always locked. The positive card is randomly shifted from one window to the other throughout the ten daily trials. The arrangement is so planned, however, that the positive card will be offered on the side of the rat's position stereotype fifty percent of the time, so that the number of rewarded jumps remains constant, as does the number of encounters with a locked win- dow and a subsequent fall to the net. If the rat persists in the position stereotype for two hundred trials of the soluble problem, it is considered to have abnormally fixated its reSponse due to having exceeded its frustration tolerance during the experiment. The criterion of two hundred trials was established by Maier as a sufficient number of trials in which to make the required discrimination, since it was found that rats rarely learned if continued in the problem beyond this number (11). In a combined report of three studies by Maier and his students (11, p. 32), 3h rats were subjected to the insoluble problem situation; only ten (29.h percent) learned the sub- sequent discrimination, while Zh (70.6 percent) fixated. Such results have led to the proposal of a qualitative differ- ence between fixations and habits (11, p. 89-91). Essentially, it is assumed that frustration resulting from the problem situation brings about a bimodal distribution of individuals; "(a) those whose position responses are modified in an average of eighty trials when an opportunity to learn was given, and (b) those whose position responses were unmodified after two hundred trials" (11, p. 89-90). The contention is that frustration serves to Split a population; the evidence for the operation of frustration in any animal is the maintenance of a position reSponse to a criterion of two hundred trials of the soluble problem. It is assumed that the introduction of the insoluble problem results in increments of frustration for the susceptible ani- imals, which separates them qualitatively from such animals as are not frustrated by the situation. The specific nature of this qualitative difference is further illustrated by a comparison between frustration- induced and motivated behavior: \ ...a frustration—induced reSponse is stronger than a response normally learned, despite the fact that the learned reaponse is reinforced by reward and the frus- trated response is not." (11, p. 91) This conclusion is based on a comparison between rats subjected to the insoluble problem and those given the following treatment: Rats are trained to a position habit under one hun— dred percent reward for from eighty to 260 trials, and are then presented with a card discrimination to be made. Most rats learn to alter their responses rather rapidly during the discrimination problem, and only a few fixate the habitual reSponse. These few are considered to have an initially low frustration tolerance (11, p. 78). Klee (10) compared the performance of two groups of rate, one of which was required to establish a position reSponse according to its natural preference to obtain one hundred percent entry and reward, the other being subjected to the insoluble problem, which randomly yielded fifty percent entry and reward. After 160 trials under these separate conditions f training, both groups were required to alter their position responses in favor of a symbol discrimination. It was found that 83.h percent of the rats which were allowed one hundred percent entry learned to make the required symbol discrimina- tion, while 75 percent of the rats exposed to the insoluble problem failed to make the required discrimination. On the basis of these results, Klee differentiates fixations from habits. It is his contention that rats which receive one hundred percent reinforcement should show more resistance to alteration of reaponse, while rats which receive only fifty percent reinforcement should have proportionately weaker habit strength in the pattern of behavior expressed, and should therefore more readily abandon their position responses when presented with a symbol discrimination leading to one hundred percent reward. The point of view which equates habit strength with consistency of primary reinforcement has been negated by the results of many investigators. In a review of the literature on partial reinforcement, Jenkins and Stanley (9) have pointed out the efficacy of such reinforcement under a variety of experimental conditions. A second objection to the dichotomy of fixation and REBEL. may be based on the amount of cue differentiation which is present at the outset of the discrimination problem. A con— trol group which receives one hundred percent reward for 160 trials is abruptly exposed to fifty percent non-entry followed by a fall to the net and non-reward. Rats so trained are con- fronted with a distinctly new learning situation, one in which stimulus-generalization decrement should occur and less compe— tition from previous learning should obtain. Entry and re- ward is always gained by jumping to a discriminable card; a fall to the net follows a jump to a different but equally discriminable card. Alteration of response is therefore greatly facilitated. On the other hand, for these rats which have been ex- posed to 160 trials of the insoluble problem, the subsequent transition to a soluble discrimination problem is a subtle one. The conditions of fifty percent reward, randomly re- ceived, still obtain. It is assumed by Maier that the change in the problem which makes one card consistently rewarding should lead to alteration of response by a goal-oriented rat. However, it has been pointed out by Eglash (2) that the rat does utilize the negative card cues on the habitual side by learning to make an abortive jump to this card. This reSponse should reduce the punishing effects of striking the locked window. Thus it appears that the rat uses the visual card cues added by the soluble problem, but uses them in a manner consistent with the pattern of response adopted during the insoluble problem. While Higard (8, p. 303-30h) has pointed out that this subtle change in the problem confronting the rat might be a crucial factor in the maintenance of the habitual reaponse, Maier has minimized its importance. In defense of his position, he introduces the results obtained with a group of rats for whom the insoluble problem was followed by a locked window, consistently blocked by the same card, on the habitual side. In this group, there was less response-alteration than in any other (11, p. hO). One hundred percent punishment produces Vonly generalization decrement, however, unless the animal shifts to the other side; then, as Maier found, in a few non- fixated animals discrimination learning is very rapid. In brief, it might be assumed that the number of fixations which occur among a group of animals depends upon the nature of the cue conditions to which the animals are exposed. One may grant that frustration occurs in the insoluble problem, but in a situation where at least two possible directions of response exist, the fact that rats respond either by learning a discrimination imposed by the experimenter or by maintaining the habitual response does not necessarily imply that frustra- tion has split the population. The ultimate criterion of abnormality in this situation must inevitably be a tautological one; failure of the animal to learn the problem set by the experimenter is sufficient evidence of susceptibility to frustration, while learning of that problem is evidence of high stability. While Maier (11, p. 28) suggests that the fixated animal may be "responding to some goal that is not under the experimenter's control", he prefers to consider the behavior as due to the Operation of a frustration mechanism, which results in a "freezing" of a sample of behavior. It is assumed by Maier that the typical position response marks the inception of behavior without a goal. He states that this response "has no adaptive value in the sense that it is adequate to the situation or in the sense that it is superior to any number of possible responses" (ll, p. 27). However, it would appear more parsimonious to assume that the same factors which lead to learning and retention of reaponse also lead to fixation. In this framework, the effi- ciency with which the animal learns to utilize the cue environ- ment of the insoluble problem situation would determine whether or not the habitual response is perpetuated. The question of normal and abnormal, as designations to be legitimately applied to rats, may be raised when the phylogenetic level of the animal is considered. Hamilton (6) has shown that the perseverative behavior of rats is much more pronounced than that of humans exposed to roughly comparable situations. It is noted by Hebb (7) that the position habit "is a constant nuisance in animal experiments" (p. 92). He suggests that the visual cues from the more dis-- tant, and therefore least variable, portion of the environment tend to dominate behavior in rats, and illustrates this point in the following manner: "Teach a rat to jump from a small platform to another one near by, the second platform is just large enough for him to land on safely, and holds food. After he has made ten jumps, move the second platform through 90 degrees. The rat hesitates, shows disturbance, but finally Jumps -- into Space, in the former direction of food. (p.93) The naive observer would be inclined to label such a performance as abnormal. It is by isolating the specific factors upon response elicitation depend that such behavior Inay be more parsimoniously explained without resorting to a diagnosis of abnormality. In Maier's orientation, the fall to the net functions solely as "punishment". It has been noted by Mowrer, (15) find Wolpe, (17) that the antecedent of such falls to the net 1J3 goading by air-blast, electric shock, or prodding with a Silick. The rat thus is involved in a situation with both Eijpproach (food reward), and avoidance (goading), components. 10 The single response develOped during the insoluble problem subserves two primary drives. When the problem is made soluble, a differential resistance to the two cards is noted. Maier says: "The differtial reactions to the positive and negative cards, shown both by the degree of resistance and the frequency of abortive jumps, reveal that the fixated rat actually has learned which card punishes and which does not. In other words, the rat has made the required differentiation but is unable to practice the required response. This property of the fixation makes it appear as a form of compulsion. The rat executes an unadaptive reaponse even when it knows better." (p. MB) The implication that the rat "knows better" has not won wide acceptance. Hilgard (8) suggests that the alternative window is no longer under consideration by the rat, so that the problem is now reduced to one window, without the alter- native possibility so obvious to the experimenter. Extrapolating from the conclusions drawn from fixation experiments, Maier and Ellen suggest "that learning theory must exclude frustrated behavior, that social psychology must distinguish between groups organized around frustration, and groups organized around common goals" (13, p. M36). Such a statement demands a radical reorientation in psychological theorization. Further investigation is necessary to determine whether "when frustration occurs, the behavior expressed is an and rather than a means to an end" (13, p, 3A6), ll STATEMENT OF THE PROBLEM In the literature on fixation, a number of factors which tend to enhance the stereotypy of reaponse sequences have been considered. It has been found that designs which necessitate a jump response by the rat (5), or which involve punishment (h), or a heightened hunger drive (3) result in a greater stereotypy of response. The fixation problem as de- signed by Maier includes all these factors. Klee (10) has considered these factors under the heading, "degree of involvement of the organism in the total situation", stating, however, that: ”In and of itself, the degree 2f involvement is not to ”be thought of as a cause of abnormal behavior. Rather, it must be considered as a condition essential in most situations for the production of such reactions only insofar as it would enable frustration to occur. From this, it would be expected that, the greater the degree of involvement, the greater the effect the frustrating situation is likely to have on the animal as measured by the abnormal reaction to the situation. Also, the frustrating situation need not be so severe where the involvement is greater." (p. S) In other words, the adherents of frustration theory -minimize those characteristics of the design which have been found in learning theory to lead to increased habit strength. The point of departure which makes fixation abnormal is posited as frustration. The question may be raised, then, whether the situation is consistently frustrating to the rat, as far as may be ascertained from the behavior of the animal, 12 apart from its success or failure in learning the problem set by the experimenter. Is it possible that the rat may be goaléoriented without learning that problem? In an experiment by Klee (10), the sole incentive to performance in the insoluble problem, and the subsequent soluble problem was a food reward. That the animals which fixated under this condition were 223 goal oriented appears to be a tenuous assumption. That consummatory responses are merely incidental consequences of successful entry to the food platform may also be questioned from the standpoint of the behavior of rats observed in the preliminary phase of the present study. Of twenty rats, only one failed to eat consistently when it gained access to the food platform. Such consummatory responses were often of such vigor that it was necessary to forcibly separate the rat from its food. Furthermore, it was observed that rats, after falling to the net, appeared to approach the experimenter, contrary to ex- pectation, since it might be assumed that an avoidance reSponse would be more apprOpriate subsequent to the "punishment" of falling to the net. Analysis of the data sheets used in previous fixation experiments employing the Lashley jumping apparatus revealed the possibility that the rat might learn to use a temporal or sequential cue during the insoluble problem. Furthermore, this cue, being uncontrolled, would continue to be effective 13 in the ensuing soluble problem, thus serving to perpetuate the pattern of response earlier developed. In the soluble problem, four standard patterns (see Figure 2, 3, Appendix) of ten trials each, are repeated over a period of two hundred trials. In the first pattern, after a fall to the net, the probability of an entry and food reward on the succeeding jump is three times the prob- ability of a fall to the net. In two patterns, the probabil- ity of entry after a fall is three to two, and on the fourth pattern, the probabilities are even, two jumps following falls to the net result in entry, and two jumps following a fall result in a second fall. If an expectancy of reward were to deveIOp on this basis, it would be randomly rein- forced in the following fashion: the animal would fall once or twice, but no more than three times consecutively, with the next jump being followed by entry and food reward. Con- versely, after entry and reward, the probability of a fall to the net on the following trial is increased in a similar ratio. Since the experimenter assumes an insoluble problem situation when he varies the visual cues, 1.9., the cards which block the two windows, his primary concern in the sub- sequent soluble problem is focused on the ability or inability of the rat to utilize the visual cues of camdsymbol and bright- ness to gain access to one hundred percent reward. The assump- tion here made is that the critical cues from the standpoint 13 in the ensuing soluble problem, thus serving to perpetuate the pattern of response earlier developed. In the soluble problem, four standard patterns (see Figure 2, 3, Appendix) of ten trials each, are repeated over a period of two hundred trials. In the first pattern, after a fall_to the net, the probability of an entry and food reward on the succeeding jump is three times the prob- ability of a fall to the net. In two patterns, the probabil- ity of entry after a fall is three to two, and on the fourth pattern, the probabilities are even, two jumps following falls to the net result in entry, and two jumps following a fall result in a second fall. If an expectancy of reward were to deveIOp on this basis, it would be randomly rein- forced in the following fashion: the animal would fall once or twice, but no more than three times consecutively, with the next jump being followed by entry and food reward. Con- versely, after entry and reward, the probability of a fall to the net on the following trial is increased in a similar ratio. Since the experimenter assumes an insoluble problem situation when he varies the visual cues, i.e., the cards which block the two windows, his primary concern in the sub- sequent soluble problem is focused on the ability or inability of the rat to utilize the visual cues of cardsymbol and bright- ness to gain access to one hundred percent reward. The assump- tion here made is that the critical cues from the standpoint of the experimenter must also be the critical cues involved in response elicitation from the standpoint of the rat. If, however, the rat learns to respond on the basis of uncon- trolled critical cues of the insoluble problem and these cues continue to lead to reinforcement during the soluble problem, then the discrimination problem posed by the ex- perimenter need not exist for the rat. The expectancy de- veloped during the insoluble problem continues into the soluble problem, maintaining the response in progress. An experiment was designed to test the possibility that such an expectancy is learned. METHOD AND PROCEDURE AIPARATUS: The standard Lashley jumping appartus was used in the present study (see photographs, Appendix). The data sheets contained a representation of the two windows to facilitate recording of the approximate nature of the jump made on each trial. Sufficient space was allowed on each sheet to record the behavior of the rat during every trial. A stOpwatch was used to record the latency of each response, from the time tne rat was placed on the stand until a jump was made. 15 SUBJECTS: Ten adult albino rats approximately 150 days old, five male and five female, were used. None of the animals had been previously used for experimental purposes. PROCEDURE: The rats were placed on a reduced daily diet of nine grams of Purina checkers and trained under a mean of nine- teen hours of food deprivation. The animals were given preliminary experience in being handled by the experimenter. After handling, they were placed on the jumping stand, which was located directly against the two windows, so that the animals could step easily to the food platform, where they were fed their en- tire daily food ration before being removed. After four days of preliminary training, the rats were required to learn to jump to the food platform from a progressively greater distance, care being taken to distribute the jumps to both windows, so that a position habit could not develop during this phase. On each trial, the two cards covered one third of each window, the cards being alternated to prevent a selection of cards during jump-training. . When all rats had learned to jump an eight inch distance to the food platform, the windows were gradually obscured by the two cards, the rats learning to knock down a card to gain access to the food. After the rats had learned to jump to 16 windows completely obscured by the cards, they were condi- tioned to make the required jump to the tap of a pencil on the jumping stand. This was accomplished by tapping alter- nately on the stand and on the rat's tail during forty trials of one hundred percent reward. This procedure was employed to reduce the amount of painful goading required to elicit the jump response during and after the insoluble problem. The animals were then given eighty trials in the insol- uble problem, in which both windows were randomly locked fifty percent of the time. No matter which window was chosen, no more than five of the ten daily trials could lead to en- try. The mean ratio of entries following falls, as compared to falls followed by second falls was twelve to five, dis- tributed in the following fashion: On the first day, the number of entries following a fall was four, the number of falls following a fall were zero. On the following two days, the number of entries following a fall was three, the number of falls followed by a second fall to the net was one. On the next two days, the number of entries following a fall was three, the number of falls followed by a second fall was two. on the sixth and seventh days, the number of entries following a fall was two, the number of falls followed by a second fall to the net was also two. On the eighth day, the simple al- ternation of falls and entries prevailed as on the first day Of the insoluble problem, to allow a comparison of performance 17 at the beginning and end of the insoluble problem, as judged by the differences in latency times on the two days. After falling, the rats were allowed to remain in the net for about thirty seconds, while the experimenter changed cards or simulated a change of cards. Upon gaining access to food, the rats were allowed to eat for about thirty seconds before being replaced on the jumping stand. The apparatus was so arranged that the experimenter could only pass the stand on the right side when going to the rear of the stand to manipulate the cards. A record was kept of the latency of reSponse, measured from the time the animal was placed on the stand until a jump was made. Activity of the rat on the jumping stand, consummatory responses after entry to food, the type of re- Sponse made, and location of the rat in the net at the time of being picked up, were also recorded. The soluble problem was based on the four standard pat- terns reported on page thirteen, with probabilities of three to one on the first pattern, three to two on the following two patterns, and two to two on the fourth pattern. The mean ratio of entries following falls as compared to falls fol- lowed by second falls was eleven to seven. The four patterns (see Figure 3, Appendix, for illustration) were rotated throughout the two hundred trials of the soluble problem. Each animal was allowed sixty seConds free time on the jumping stand. Failure to jump within that time was followed 18 by thirty seconds of tapping on the stand. After a total of ninety seconds on the stand, the animal was subjected to actual goading by being tapped on the tail with a pencil un- til a jump response was made. Animals who learned the discrimination problem to a criterion of fifty successful entries were presented with a ckuaice between the discriminated positive card, which was rwuidomly locked fifty percent of the time, and.an Open window, 'with.no card displayed. This procedure was employed to test the possibility that failure to jump to an open window was :not exclusively a characteristic of only such rats as fixated. 19 PHESEQTATION OF RESULTS All animals continued to eat throughout the experiment. All ten rats developed position stereotypes, and at the <3onclusion of the insoluble problem were presented with a <3ard.discrimination, permitting one hundred percent reward. wao rats learned the card discrimination, one after forty ‘trials, the other after seventy trials. One rat remained ‘variable throughout the experiment. Seven rats reached the fixation criterion of two hundred trials. Two fixated rats developed unique patterns of response, 'which.took the form of exerting extreme force during jumping. Owing to the design of the problem, this reSponse resulted in .fifty percent successful entries, and thus might constitute a ferm of solution to the rat. One animal abandoned this iform of resyonse after 120 trials in favor of an abortive SPesponse to the negative card; the other maintained extreme iforce in jumping throughout the problem. The difference between the median latency on the jumps following a fall to net and the median latency of the jumps followed by entry was computed for the first ten trials and the last ten trials of the insoluble problem. On the first ten trials, this difference was in the direction of longer latencies for the jumps following falls. On the last ten trials, the difference was reversed, and the difference between these 2O differences is significant at the one percent level of confidence (see Table I, Appendix). Thus it may be inferred that learning to the suggested cue took place during the in— soluble problem. That such learning affected the behavior of the animals during the soluble problem is indicated by the latencies of the fixated rats when confronted with the nepative card on two or more successive trials. It was found throughout the soluble problem that the animals showed decreased delay in jumping to the negative card after a fall to the net. In other words, a fall to the net appears to result in a faster jump on the following trial, even when the negative card has appar- ently been discriminated from the positive, in terms of longer latency times and abortive style of jumping. For the eight non-learning animals, the mean time of the first jump to the negative card was h9.6 seconds; the mean time of the following jump to the negative card was 3h.2 seconds (see Table II, Appendix). This difference is significant at the 0,1 percent level of confidence. It was also found that animals jumped more rapidly to the positive card after falling to the net than after eating. Mean latency of the second of two successive jumps to the positive card was 28.1 seconds; mean latency of jumps to the positive card following falls to the net was 19.7 seconds. This difference was also significant at the 0.1 percent level of confidence (Table III, Appendix). A comparison of the latency of reSponse in those 21 daily trials in which the positive card was presented two or more successive times shows a greater latency on the second presentation. Mean latency on the first jump to the positive card was 22.6 seconds; as noted above, mean latency on the second successive jump to the positive card was 28.1 seconds. This difference was significant at the one percent level of confidence (Table IV, Appendix). It should be specifically noted that the first jump to the positive card is in most instances preceded by a trial which resulted in a fall to the net. Figure I (Appendix) illustrates these latency comparisons in a typical pattern of ten trials taken from the record of one rat. With succeeding trials, the rats, after falling to the net, also appeared to learn a particular approach reSponse to the experimenter. The experimenter always appeared from behind the apparatus on the right side after changing the cards (or simulating a change of cards) on each trial. Prior to the forty-first trial of the soluble problem, the mean number of times the rat was located at the right edge of the net at the time of being picked up was lh.h times at the right edge and hl times elsewhere in the net. After the forty-first trial, the mean location at the time of being picked up was 53.5 times at the right edge and 16.5 times else- Where in the net. Thus, it would appear that the rat is goal- oriented toward the experimenter and toward the jumping stand subsequent to a fall to the net. 22 While it is known that a fixated rat will jump to a locked window on the habitual side in preference to an open one (no card present), this test has apparently not been made with rats who have learned the problem. After fifty success- ful entries, the two rats which learned the discrimination were presented with a choice between the positive card, which was now locked fifty percent of the time and an open window. One rat jumped to the card 28 times out of thirty, falling 1h times. When presented with a choice between the negative card and an open window, the rat jumped to the open window five out of six times. Presented with the positive card again, the rat chose the card six times out of seven, falling three times. The other rat chose the open window in preference to the positive card, jumping with little delay. However, when presented with a choice between the negative card and an open window, the rat required goading on two successive trials to elicit response, although it jumped to the open window on each trial. Thereafter the rat jumped easily to the open window in the presence of either card. One rat (Figure h, 5, Appendix) began to abort more strongly to the negative card during the closing trials of the experiment, until it began to fall to the floor beyond the left side of the net. After two falls to the floor, the rat began to jump to a cage 2h inches away when confronted with the negative card on the habitual side, but it did jump 23 to the window on that side when the positive card was displayed. It was found that even when a negative card was removed from the window, allowing obvious access, the rat still jumped to the cage. Evidently, the positive card on the non-preferred side is sufficient to signify a locked window, since it is always present on the non-preferred side when the locked negative card is presented on the habitual side. Evidence of frustration during the insoluble problem, as inferred from the behavior of the rats: Two of the rats in the non-learning group made attempts to escape from the jumping stand by jumping to the floor early in the problem. This behavior occurred during one trial in each instance. One of the animals who subsequently learned the soluble problem aborted to the left of the left window seven times in succession on the second day, and three times in succession on the fourth day. Thus, as far as may be determined from the behavior of. the rats, the problem may be frustrating to those which learn as well as to those which do not. It may also be significant to note that this latter rat continued to show increased latency of reSponse after falling until it learned the problem. At the conclusion of the experiment, a method of breaking fixation was devised and applied to five of the fixated animals. This was a generalization technique, in which the habitual re- sponse was gradually moved over to the non-preferred window. It is best understood by study of the photographs, Figures 7-15, in the Appendix. The procedure was found effective for breaking the fixation in all animals in terms of entering the open window on the non-preferred side. No attempt was made to utilize the technique in training animals to make the card discrimination. DISCUSSION OF RESULTS While all of the animals continued to eat throughout the experiment, consummatory behavior is presumably not sufficient evidence to diaprove the theory of behavior without a goal. However, when the experimental findings indicate a sequential relationship exists between those trials which are rewarded by entry and those which result in a fall to the net, the frustration-fixation theory is seriously jeopardized. Since the responses learned to the cues of fall and entry have received a large number of reinforcements during the insoluble problem, and are reinforced more often than the positive card one during the soluble problem, that is, more than fifty percent of the time while the rat maintains a position pref- erence, there seems to be no reason why a rat which has learned approach and avoidance responses to these cues should not remain fixated. It is plausible to assume then, that the rat has maxi- mally employed certain cues during the insoluble problem, which, owing to the physical design of the subsequent soluble problem, continue to be effective in maintaining the habitual response. That the majority of rats exposed to these conditions do not learn the problem set by the experimenter is thus not necessarily a sign of abnormality. It may be argued, however, 26 that the rats have not solved gay problem: fixated rats continue to perform under fifty percent reward, even though a differential cue exists which can lead to one hundred percent reward. Such a position demands a certain prescience on the part of the rat, a certain expectancy about the be- havior of experimenters. The expectancies developed by the rat appear to be based on its own responses in a situation, not considered as a problem per se, but as a maximal adjustment to a situation to which the animal is exposed. That such an adjustment occurs during the insoluble problem is affirmed by Maier, who states however, that "Any adjustment accomplished must be regarded as purely incidental, and not as a factor that contributes to the development of fixations" (ll, p. 53). If such an adjustment is based on uncontrolled cues utilized by the rat, there would seem to be no valid reason for main- taining that it "must be regarded as purely incidental". Further, Maier and Feldman (1h) found that a fixated response is stronger when the rat is exposed to 160 trials of the in- soluble problem than when it is subjected to eighty, and that more fixations resulted from the longer Series of trials. It was supposed that frustration produced "increments of rigidity" (11, p. 65) which strengthened the position response. From the standpoint of the present analysis, the cues which establish an expectancy of entry after a fall to the net would be more frequently reinforced during a longer series of trials, thus both strengthening'the habitual reaponse and increasing the number of fixations. 27 Since the sequential cue of entry after fall continues to be reinforcing during the soluble problem, even though a card discrimination is offered which leads to one hundred percent reward, the continuation of the habitual reaponse need not be considered abnormal. Two possibilities of response exist, one which is arbitrarily defined as learning 3 problem, the other being labeled fixation. Statistically speaking, the normal response to the problem is fixation, since the majority of animals maintain the habitual response throughout the problem. There is no reason to suppose that in a complex situation all animals should learn the reSponse required by the experimenter in order to be considered as normal. The two rats which employed extreme force in jumping illustrate the point that even fixated rats may not all be— have alike. Although exposed to the same objective situation as the others, these rats jumped so vigorously that each entry invariably carried them to the rear of the food plat- form. Impact against the locked window was much harder than that of the other fixated rats. But since extreme force in jumping resulted in fifty percent successful entries, in a random order of reinforcement, it could well constitute a form of solution to the rat. In each case, this reaponse was also combined with decreased latency of response after falling to the net. Failure of fixated rats to jump to the non-habitual window even when no card obstructs the view of the food platform, 28 has been taken as an indication of a compulsive abnormality (11, p. 53). It might be expected, then, that rats which learn the required discrimination, being "normal", could easily jump to the Open window when circumstances made such a response "desirable". Yet one of the rats which learned the discrimination continued to jump at the positive card in preference to an open window even when such jumps led to falls to the net on half the trials, as described on page twenty-two. This rat did not alter its response in favor of the open window until after a total of 1? falls to the net. Such behavior indicates that fixation is not necessarily qualitatively different from a fairly well learned reSponse. That fixation does not represent a compulsion to jump to a specific card is demonstrated by the behavior of rats when the jumping stand is shifted to a point midway between one of the windows and the blank face of the apparatus (Figure 8, Appendix). Given a choice between the positive card in the window toward which the habitual reSponse is made, and a blank wall, the rat jumps against the wall. Even without a card in place in the habitual window (Figure 9, 10, Appendix), the rat still jumps against the wall. While such behavior appears strange, it must be remembered that it is the behavior of initially naive rats which have practiced a highly specific response over a period of more than two hundred trials, in a limited environment. A similar form of behavior may be observed in the human infant of nine or ten months, according to Piaget: 29 "When the baby is placed between two.pillows and he has succeeded in finding an object hidden under the right one, the object can be taken from his hands and placed under the left pillow before his very eyes, but he will look for it under the right pillow where he has already found it once before, as if the permanence of the object were connected with the success of the former action, and not witg a system of external displacements in space.” (16, p. 3 - In the same sense, it is the rat's response, practiced over many trials, which has become highly specific to the situation. Whatevever the cues are which lead to response elicitation, they are not necessarily the symbol cards which block the windows. As in Hebb's experiment, previously cited (p. 9). the visible cues of the goal need not be present to elicit the habitual response, as long as the general environment is sufficiently stable to allow the establish- ment of the orientation from which the response is to be made..When the response is allowed to generalize to the othervwindow, response to that window is easily made, in the presence of either card in the habitual window (as illustrated in figure 15, Appendix)..As has been suggested by Hilgard,, it appears that fixation is actually a failure to generalize, and not the expression of a compulsion. That the responses of fixated animals are compulsive in nature may also be questioned on the basis of the atypical response alteration of the rat whose abortion resulted in a fall to the floor..After two such_experiences, it was quite able to alter its response, expressing no "compulsion" to Jump to the negative card. It is significant also that the 3O rat elected to jump 2h inches to the cage instead of to the positive card on the non-preferred side. Since the rat was not compelled to jump to the negative card, the question may be raised as to why it did not jump to the other, which had apparently been discriminated as the positive card. It would appear that the positive card on the non-preferred side may come to serve only as a cue of non-entry, since it is always in the other window when the rat jumps to the negative card. This hypothesis is further supported by the behavior of the rat when the negative card is removed (Figure 5, Appendix). The rat still jumps to the cage although "obvious" access is afforded. The positive card on the right side appears to be sufficient as a cue of expectancy of a locked window, eliciting the response of jumping to the cage. The behavior of rats following a fall to the net adds supporting evidence to the hypothesis that an expectancy of entry and reward after a fall is developed and maintained by the underlying cue conditions inherent in the design of the problem. The implication that the rats invariably made an approach response toward the experimenter after falling is difficult to reject. Such approach responses often occurred directly after the appearance of the experimenter from behind the apparatus. That such movement toward the experimenter was related to an expectancy of food reward is suggested by the following: In working with rats which have been placed on a reduced diet investigators commonly experience being bitten by the animals. Such biting may range from tentative "nips" to skin-puncturing bites. Since the experimenter is the person who feeds the animals, such "biting of the hand that feeds" may be assumed to be an anticipatory food response. The hand is used in placing the daily ration of food in the cage, and is therefore both a visual and olfactory cue followed by food reward. One animal (number A, Table II, Appendix) showed this biting tendency to a marked degree when fed in the cage, seizing the hand of the experimenter each day as food was placed in the cage and relinquishing it when the food pellets were dropped on the cage floor. Over the last forty trials. of the soluble problem, this animal was offered the hand of the experimenter at two points: (1) when picked up from the food platform after successful entry and food reward, (2) at the right edge of the net following a fall. After eating, when the probability of entry on the next trial is lower, this rat bit the offered hand three times; after falling and approaching the right edge of the net, the animal bit the of- fered hand thirty times. Such reaponses could of course be taken for "hostility" on the part of the rat, but since the rat desisted after two or three bites, and did not indulge in such behavior to an appreciable extent after eating it is more likely that the response was an expectancy for entry and food reward after a fall. Thus, although only one rat was tested lIl'thlS manner, the approach response of all animals to the _rigjit edge of the net could also be considered as expectancy cufiiented. In other words, in spite of having failed to learn this problem imposed by the experimenter, the rats 3“305Ted {final-oriented on the basis of cues other taan those assumed to Ice the most critical cues of the situation. The present pussition is perhaps best summed up in an observation made by Maier: "Before one can conclude that an animal has failed to learn, one must be sure that it hasn't learned something different from what was intended." (12, p. 22) Analysis of the data sheets used in previous fixation experiments suggested the possibility that rats might learn to use a sequential cue during the insoluble problem. It is possible that a fall to the net may serve as an important cue because of the increased probability of entry and reward following such a fall. Owing to the pattern of card presenta— tion, the cue would continue to be effective in the ensuing soluble problem, serving to interfere with the learning of a card discrimination. Results of the present experiment indicated that such a cue operated in the manner suggested. Rats jumped more rapidly on trials following a fall to the net on the last ten trials. During the soluble problem, rats jumped more rapidly on the next trial following a fall to the net regardless of whether the next trial offered entry and reward or another fall to the net. Although eight of ten rats failed to learn the required discrimination, reaching the fixation criterion of two hundred trials of the soluble problem, these animals appeared to be goal-oriented throughout the problem. They continued to eat on each trial when entry was made, and subsequent to a fall to the net, they learned to make approach response to the experimenter. Of the two rats who learned to a criterion of fifty successful entries, one continued to jump to the positive card in preference to an open window, even when the positive card was locked fifty percent of the time, resulting in a fall to the net on half the trials. While this animal ulti- mately learned to jump to the open window, it may be inferred that similar behavior in fixated rats is not qualitatively different from such fairly—well learned reSponse. The results of the present study offer evidence against the view held by Maier and his associates that fixated be- havior is "behavior without a goal." APPEND IX 35 TABLE I. MEDIAN LATENCY BEFORE FALL MINUS _ MEDIAN LATENCY AFTER FALL ON DAY ONE AND DAY EIGHT OF THE INSOL- UBLE PROBLEM. (TEN RATS) Rat Day 1: Day 8: Difference: 1. -27.5 seconds 12.5 seconds 40 2. - 0.5 " 3.5 " 4 3o: '36 n 1 n 37 2.. -33 3 g 3 3g .. - 9 - 6. 13 fl _ 2 ll _15 7.. ~43 " - l " 42 8. - 5.5 " 7 " 12.5 9 e — -5405 n -16 II 38.5 10. “2205 N . 12 .5 N 42 Mean: -22.5 ' 2.15 24.7 The number on day one should be algebraically larger than on day eight,.as it invariably is.. t ratio: 3.53. Differences are significant at the 1% level of confidence. *- fl' * TABLE II. COMPARISON OF MEAN LATENCIES OF THE EIGHT NONALEARNING RATS ON TWO OR THREE SUCCESSIVE PRESENTATIONS OF THE NEGATIVE CARD ON THE HABITUAL SIDE DURING THE SOLUBLE PROBLEM. Rat Mean time of first mean time of 2nd Jump to neg. card: Jump to neg. card: 1. 39.3 seconds 26.9 seconds 2.. 30.9 " 23.9 " 3.. _ 36.1 " 26.2 " 4.. 42.5 " 22.5 " 5. 49 .9 fl 3704 II 6.. 71.5 " 51.1 " 7.. 48.1 " 35.5 " 8. 10.2 " 50.2 " Mean: 48.6 34.2 t.ratio: 7. 55. Difference between means is significant at the 0.1 level of confidence.. 36 TABLE III. COLPARISON OF MEAN LATENCIES OF THE EIGHT NON-LEARNILG RATS OL PRLSEH— TATION OF THE POSITIVE CATZD OL THE HABITUAL SIDE AFTER ENTRY AND AFTER FALL DURING THE SOLUBLE PROBLEM Rat Mean time of jump . Mean time of jump to pos. card after to pos. card after a previous entry: a previous fall: 1 2S. 6 seconds 16.1 seconds 2 18.1L " 1L1 " i 23 7 n 13 6 n 13.7 II 2%:5 H 5 '7 fl '9 n 6 2.0 n 33.2 n 7 39.7 .. . 33.9 n ,8 22.1 18.2 Mean: 28.1 19-7 t ratio: 7. 21. Difference between means is significant at theCLl%level of confidence. TABLE IV. COMPARISON OF MEAL LAlW JQCIES Or THE EIGHT NON-LEARNING RATS OL TH'O CR THREE SUCCESSIVE PRLSENTATIQLS OF TIE I‘OSITIVE CARD Om TIIE HABITUAL SIDE DURILG THE SOLUBLE ILLSLLL Rat Mean time of Mean time of first jump to second jump to pos. card: pos. card: 1 18. 2 seconds 25. 6 seconds 2 17:5 n 18 u n g lh.8 n 23 7 n 5 33.8 n ' 15.; n 6 3827 I: -220 g 7 34.2 .. 39.7 .. 8 21.g 22.1 Mean: 22.6 28.1 t ratio: H.29. Difference between means is significant at the 1% level of confidence. Windows Wb ;- @714? 28 Bw - Kb 12 Bw -'EQ 22 Wb - §g_ 15 Bw - E 5 Bw - Eb I7 Wb - E! 17 Bw e E2. 4 Wb - 2!. 14 Wb - §!_ 7 Fig.4 latency time: (in~seconds) 37 Rat strikes locked window, falls to net. Rat enters right window, eats. Rat enters right window, eats. Note increased latency time on second successive entry. Falls to net.. Entry after fall..Note decrease in latency time after a fall.- Entry after entry, increased latency time.. Rat strikes locked window on* right, falls to net. Rat enters right window, eats. decreased latency after a falla Rat strikes locked window on right,,falls to net.. Second successive Jump to a locked window. Note thatzlateny cy time is decreased following a fall to the net,,even:when the non-entry card is display- ed in the habitual window.. l..IdeaI?expectanny pattern~illustrating variation in latency times after entry and after falling to the net..This is the performance record of a rat fixated on the right, for ten daily trials. *Bw means "Black card with white circle";.Wb stands for "White card with black circle".- For this rat,,Bw was always locked,,an either side, while Wb was always unlocked,.allowing entry to food on either side. Symbol on the left refers to left window; symbol on the right refers to right window. Bw 5 Fall Entry Fall Fall Entry Entry Fall Entry Entry Fall 38 Both windows locked.. Both windows open..Entry after fa11.e Fall, trial 3, followed by 2nd fall.. Entry after fall.. Entry after entry.. Entry after fall. falls to the net are followed by entry; one fall to the net is followed by a second fall.. L 2.. 3. 5: a 7- a- 9. 1m 5§$$¥§§$§¥ $3§§§$9§$§ Entry Fall Fall Entry Fall Fall Entry Entry Entry Fall Fall, .trial 2,.followed by second fall. Entry after fall. Fall, trial 5,.followed by seoOnd fall. Entry after fall.- Two falls to the net are followed by entry; two falls to the net are followed by a second fall.. 1. Bw 2.~Wb 3. Wb 4. Wb 5. Bw 6..Bw 7..Wb 8..Wb 9. Bw Three Wb Bw . Bw Bw Wb~ Wb Bw Bw Wb Wb Fall Fall Fall Entry Entry Fall Entry Fall Entry Entry Fall, Fall, Entry Entry Entry trial 1, followed by second fall. trial 2, followed by third fall.. after fall.. after fall. after fall. falls to the net are followed by entry; two falls to the net are followed by another fall.. Fig..2.. Illustrating certain typical sequential prob- abilities of entry and reward during the insol- uble problem, when both windows are randomly locked 50% of the time, so that neither card can lead to 100% entry and reward.. 39 1. Wb - §w_ Fall 2..Bw - Wb Entry Entry after fall.. 3. Bw - Wb Entry 4..Wb - 21, Fall 5. Bw - Wb. Entry Entry after fall.. 6. Bw - Wb Entry 7e Wb - §E{ Fall 8. Bw - Wb Entry Entry after fall. 9.;Wb - BE. Eall 10. Wb - Bu Fall Fall,,tria1,9,,followed by 2nd fall.. Three falls to the net are followed by entry; one fall to the net is followed by a second fall.. A—__ 1e\BW - Wb Entry 2..wu.- g! Fall A -3..Bw - wb Entry Entry after fall. #O.Wb - a! Fall 5..Wb - 31 Fall Fall, trial 4,,followed by 2nd fall. 6..Bw - Wb Entry Entry after fall. 7e-Bw "' Wb mtry 8e Bw - Wb Entry 9..Wb - g1 Fall -Wb - 2! Fall Fall,,trial 9, followed by 2nd fall.. Two falls to the net are followed by entry; two falls to the net are followed by second falls.. 1e Bw ‘ Wb Entry 2. Wb - 2! Fall 3..Wb - E! Fall Fall, trial 2,.followed by 2nd falls 4..Wb - Bw Fall Fall,trial Bufollowed by third fall.. 5. Bw - WE Entry Entry after fall. 6. Bw - Wb Entry 7e Wb - E! F311 8. Bw - Wb Entry Entry after fall. 9..wn - 2!, Fall . 10. Bw — Wb Entry Entry after fall. Three falls to the net areFfollofiEd'by‘entry;"two'falls to the net are followed by another fall. Fig..3.. Illustrating the sequential probabilities of entry and reward after a fall to the net dur- ing the soluble problem, for a rat fiXated on the right. Although the Nb card is always une locked” in either window, the sequnltisl one of entry after a fall to the net is still in operation.. Fig. 4. This rat,.shown making an abortive entry to its pos- itive card,,began to abort more strongly at the close of the experiment when confronted with the negative card on the left side. It began to miss the net entirely, falling to the floor. After two such falls, it began to Jump to a cage 24 inches a- way (see below) when confronted with the negative card. It con» tinned to Jump to the positive card on the left side, however, as shown. Fig. 5. It was found that even when the negative card was re- moved from the habitual window,. the rat still Jumped to the cage. It appears that the positive card on the nonrpreferred side may come to serve only as a further cue to the situation of a locked window on.the habitual side, e- liciting an:avoidance res onse even when that window is obvious- ly" open. Fig. 6. A rat at the right edge of the net,.exhibiting the typical orientation to- ward the experimenter, who always appears from behind the apparatus on the right side. 41 GENERALIZHTION PROCEDURE USED IN BREAKING FIXATION Fig. 7. A rat making an abortive Jump to the negative card. For this rat, the negative card, which is always locked, is the black card with the small white circle.. The Jumping stand is eight inches from the windows.. ‘ Fig. 8..When the Jumping stand is shifted to the right,.the habitual window assumes the relative posi- tion of the nonspreferred window..1 The rat cannot be claimed to be expressing a "compulsion" to Jump to the negative card,.since it is not present..The elicited response is that which has been practiced in the past.. Fig. 9..Ewen.with no card pres- ent in the window, the habitual response is made, and occurs without goading. The rat is shown here Just at the point of leaving the stand, as it Jumps to the wall. GENERALIZKTION PROCEDURE (CONTINUED) 42 Fig. 10. With the Jumping stand directly before the habitual wine dow, the fixated response is - still elicited. The determinants of response here seem to be 1hr ternalized rather than objective. Fig. 11. With the stand returned nearly to the normal position,. the rat Jumps readily to the ha- bitual window.This trial is fol- lowed by one in which the stand is in the normal position (not shown). The Jump is also made to the habitual window.. Fig. l2..With the stand again~ directly before the habitual wine dow,.the response is now directed to the window. The cues of the ex- ternal environment,.on the basis of the previous responses, are be- ginning to determine the response. 43 GENERALIZATION PROCEDURE (CONCLUDED) Fig. 13. With the stand placed as in figure 9, the rat still Jumps to the window, although it previously could not do so. In terms of the relative posi- tion of the habitual window, the response may now be cone sidered as being made to the non-preferred window. Fig..l4. Even when the stand is placed far to the right, the rat still enters the window. Fig. 15. With the stand restored to the normal position, the lock- ed negative card in place in the habitual window, the rat is now able to enter the non-preferred window. The rat can also enter the open nonspreferred window in the presence of the positive card in the habitual window (not shown). The response has been generalized to the other window. 10. ll. 12. 13. BIBLIOGRAPHY Eglash, A.£ Abnormal fixations. Unpublished Ph. D. thesis, University of Michigan, 1951. Eglash, A.: Perception, association and reasoning in animal fixations. Psychol. Rev., 1951, vol. 58, EZh-hBh. Elliott, M. H.: The relationship of drive to learning. Psychol, Bull., 1932, vol. 29, 6AA. Farber, I. E.: Response fixations under anxiety and non- anxiety conditions. g, Exp. Psychol., l9h8, vol. 38, 111-131. ‘ Gilhousen, H. C.: Fixation of excess distance patterns in the white rat. Q. Comp. Psychol., 1933, vol. 16, 1-23. Hamilton, G. V.: A study of perseverance reactions in primates and rodents. 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