Iowa Turfgrass Research Report I o w a S ta te U n iv e r s it y University Extension Department of Horticulture Department of Plant Pathology Department of Entomology Cooperative Extension IOWA STATE UNIVERSITY Ames, Iowa FG-466/July 1999 In Cooperation with the Iowa Turfgrass Institute Field Day Program - July 29,1999 9:00 a.m. Introductory Remarks - R egistration Tent 9:30 a.m. CHOICE OF FOUR TOURS All tours start from registration area. See following two pages for specific topics, speakers, times, and locations. Tour #1 Lawn Care & Grounds — Kentucky bluegrass traffic studies, prairie plants, seed establishment, shade study, crabgrass and corn gluten meal weed control, disease control, mower maintenance. Tour #2 Golf Course —Bentgrass varieties, crabgrass and com gluten meal weed control, sloped green study, disease control, plant growth regulators, seed establishment, mower maintenance. Tour #3 Sports Turf — SportGrass and Enkamat, tarp color demonstration, Kentucky bluegrass traffic studies, bermudagrass/ryegrass, crabgrass control, disease control, mower maintenance, seed establishment. Tour #4 Landscape —Selection and care of landscape plants for Iowa. 11:30 a.m. 1 :0 0 p .m . Lunch (Served in Exhibit Area) Educational Sessions and Demonstrations ♦ Pesticide Recertification Cont. Ed. Course (2 hours) —Main Building ♦ T\irf I.D. and Weed, Disease & Insect Control Tour — Dr. Dave Minner and Dr. Nick Christians ♦ Vendors and Equipment —Exhibit Area Wildflower Native Grass Establishment Study c oa uI * SD Common KBG Parade KBG Com Gluten Weed Control Trial Glade KBG u y >o p !§ sg High Maintenance Bluegrass 1995 Park KBG Ram I KBG Corn Gluten Weed Control Trial ISU Blend P.Rye Tall Fescue Seed Establish­ ment Studies 8 A IL Perennial Ryegrass Cultivar Study Adelphi KBG 100' Bermudagrass / Traffic Study I ? Native Soil Penncross Bentgrass Green * © g € ■O Ä 0 CD C_ ü c I jQ c o 0 0 00 CCQQ 0 0 0 0 > 0 E 2 £ 0 ^ CD O ) o 0 D JQ 0 0 0 $ 0 C o 0 0 O * '4h8 C CO I 57=E c° o f LU *8 oc Ì CcO 0 '‘ c CO TO £ CQ CO 05 -Q CO CL o tr o CO 0 iz CO og co E ^ 0 $ o 2 4L C "O 4L 0 £ 0 0^ c- CO c 05 § C ^ C O -S cà o co ^ 0 Oi 0 > § < £ ^ 0 O) 0) © 0 £ £ 0) £ 0 = >* 0 ^ co Q _ -Q 05 15 0 0 2 > 00 TJ 0 CD o O 3 "O E i— C 0 0 0 0 CD *: CO nin CD I« II 4L 4L c 1 — 0, Cr O .0 2 Í ¿3 CQ _ CD C O iS § * O <0 0 ¿ s 0G 0 -O 1 ü -g 25 "" c I 0 TO o 0 £ CQ 0 -C o 0 0 0 ^ 0 $ ■o CD -àç O) 0 0 _Q CL 0 T3 0 O 0 0 0 0 Ö Ico CO m 0 3 od 01 § O fl O O) c _ CD E-co © 0 > C O 0 o 0 o is £ 'S c I O CL o o 0 0 0 c 0 0 0 0 o CL OC 0 0 4L 05 4L 2 c 20 .© 8 O ^ O ) -2 CD -C *- 0 0 33 0) 0 > CD 0 ■§ .5 0 c: 0 O JC O 1$ £ c O) 0 CQ CO 4L O c o 0 co :s tf ¿S 0 00 05 Managing bentgrass stress on putting green slopes. !fc C Shade adaptation study. =0 > Uj Deying Li, ISU Horticulture to co Gary Peterson, ISU Extension «O QJ Managing cool-season grasses as part of a SportGrass system. Stabilizing sand based athletic fields with Enkamat. li* Jay Hudson, ISU Horticulture I S I Pre- and postemergence control of crabgrass. III Natural products for weed control c S Melissa McDade, ISU Horticulture Paul Stevens, ISU Horticulture S § l Barbara Bingaman, ISU Horticulture Plant growth regulators Turf response to tarp covers CD Disease control studies - pythium, dollar spot, and summer patch. CM CO 'M- m Mark Howieson, ISU Horticulture 0 Mark Gleason, ISU Plant Pathology Bermudagrass/ryegrass O Dave Minner, ISU Horticulture CO ÌZ vV 05 Seed establishment study CO NTEP Kentucky bluegrass variety trial - traffic. CO "g Dave Minner, ISU Horticulture 0 co Young Joo, ISU Horticulture ii Fairway and putting green height bentgrass variety trials. c Prairie plant demonstration. O Q) g S’ o _0 CL Nick Christians, ISU Horticulture SB c -CCO c o co o cn 0 CO > ® 'S' $ LU © 0 ¿5 co a) m m § « o> ~ ro C § £ É 3 In-season mower maintenance. 2 t f - *CO Paul Stevens, ISU Horticulture o Great American Outdoor 0 c Topic Landscape Tour 0 C <0 5 o £ Speaker Tour o £ 0 o ■o c CM CO O T— Introduction Nick E. Christians and David D. Minner The following research report is the 20th yearly publication of the results of turfgrass research projects performed at Iowa State University. Copies of information in earlier reports are available from most of the county extension offices in Iowa. This is the third year that the entire report is available on the Internet. It can be accessed at: http://www.hort.iastate.edu/hort/pages/faculty/g_frame.html Several new projects were started in the 1998 season. The fine fescue trial was replaced with a new trial that includes 79 species and cultivars. The fairway-height and green-height bentgrass studies were replaced with the newest cultivars from the National Turfgrass Evaluation Program (NTEP) in September. A new fairway height bluegrass study was also added for the first time. Other new research sites, including SportGrass - a combination of natural grass and synthetic turf, Heatway - a water circulated soil heating system, SubAir - a subsurface forced air system, several organic and inorganic sand amendments, and a sloped area to study temperature and moisture stress on putting greens were also completed in 1998. We would like to acknowledge Richard Moore, superintendent of the ISU Horticulture Research Station; Jim Dickson, manager of the turf research area; Barbara Bingaman, Postdoctoral researcher; Doug Campbell, research associate; Dr. Young Joo, visiting scientist; Jay Hudson, Deying Li, Mike Faust, Mark Howieson, Rod St. John, and Melissa McDade, graduate students; and all others employed at the field research area in the past year for their efforts in building the turf program. Jim Dickson left the manager position in 1998 and Rod St. John was hired into the position in 1999. We would like to thank Jim for his years of service to the program. Special thanks to Lois Benning for her work in typing and helping to edit this publication. Edited by Nick Christians and David Minner, Iowa State University, Department of Horticulture, Ames, IA 50011-1100. Dr. Nick Christians Phone: 515/294-0036 Fax: 515/294-0730 E-mail: nchris@iastate.edu Dr. David Minner Phone: 515/294-5726 Fax: 515/294-0730 E-mail: dminner@iastate.edu Table of Contents Environm ental D ata.......................................................................................................................... 1 Species and C ultivar Trials Results of Kentucky Bluegrass Cultivar Trials............................................................................ 5 Regional Tall Fescue Cultivar Evaluation.................................................................................. 10 Regional Fine Fescue Cultivar Evaluation.................................. ................................................ 13 Perennial Ryegrass Study............................................................................................................. 16 Shade Adaptation Study................................................................................................................ 18 Fairway Height Bentgrass Cultivar Trials................................................................................... 21 Green Height Bentgrass Cultivar Trial (Native Soil)................................................................. 23 Overseeding of Northern Turfgrass Sports Fields with Bermudagrass....................................... 25 H erbicide and Growth Regulator Studies Preemergent Annual Grass Control Study.................................................................................. 27 Postemergent Annual Weed Control Study................................................................................ 32 Postemergent Broadleaf Weed ControlStudy.............................................................................. 36 Postemergent Granular & Sprayable Broadleaf Weed Control Study....................................... 40 Postemergence Ground Ivy Weed Control Study at Veenker.................................................... 48 LCO Weed Control Study............................................................................................................ 50 Poaannua Control Studies - 1998....................................................................................... 55 Effect of Beacon on the Germination of Kentucky Bluegrass and Creeping Bentgrass......... 57 Fairway Bentgrass Growth Regulator Study................................ 60 Effect of Trinexapac-ethyl on Kentucky Bluegrass Sod Establishment.................................. 63 Effects of Trinexapac-ethyl on Poa annua Populations in Green Height Creeping Bentgrass.............................................................................................................. 66 Effects of Trinexapac-ethyl on Poa annua Populations in Fairway Height Creeping Bentgrass............................................................................................................... 69 Turfgrass Disease Research Evaluation of Fungicides for Control of Dollar Spot on Creeping Bentgrass......................... 71 Evaluation of Fungicides for Control of Pythium Blight on Perennial Ryegrass.................... 73 Evaluation of Fungicides for Control of Brown Patch in Creeping Bentgrass........................ 74 Fertilizer Trials Creeping Bentgrass Establishment and Management on Sand Greens..................................... 76 E nvironm ental Research Corn Gluten Hydrolysate for Weed Control.............................................................................. 79 1991 Corn Gluten Meal Crabgrass Control Study - Year 8 ....................................................... 80 1995 Corn Gluten Meal Rate Weed Control Study - Year 4 ..................................................... 84 v Turf Management Select® Liquid Ice Melter Study.................................................................................................... 90 Soil Modification and Sand-based Systems Stabilizing Sand-based Athletic Fields with Enkamat................................................................. 92 Managing Cool-season Grasses as part of a SportGrass® System............................................... 97 Managing Bentgrass Stress on Putting Green Slopes.................................................................. 104 Effects of Inorganic Soil Amendments on Sand-based Media................................................... 106 Modifying Athletic Field Soils with Calcined Clay and Tillage................................................. 109 Athletic Field Turfgrass Response to Calcined Clay Topdressing............................................ 111 The Effect of Tarp Color on Turfgrass Growth........................ ................................................ 112 Calcium Applications to Turf on Sand-based Media.................................................................. 114 Ornamental Studies Effect of Organic and Mineral Mulches on Soil Properties and Growth of Fairview Flame® Red Maple Trees...................................................................................................... 115 Prairie Demonstration................................................................................................................. 119 Introducing The Iowa State University personnel affiliated with the Turfgrass Research Program.......... 120 Companies and Organizations that made donations or supplied products to the Iowa State University Turfgrass Research Program........................................................ ................... 121 Environmental Data > ^ O £ ox p t 00 ON O t-H 1-H CN CO t o VO r - 00 r-H i—H 1—H r—H M— 1 r-H r — < fH ON r -H t o VO OO ON o r-H o r — < F=0.07 1.1 6.7 6.7 7.7 6.7 7.0 6.3 6.0 7.3 7.3 6.0 5.0 7.3 6.0 5.3 7.0 5.7 5.3 5.3 5.7 5.0 5.0 1.0 7.0 7.7 7.7 6.7 6.7 7.3 6.7 7.0 6.7 6.3 6.0 6.7 6.0 6.0 6.3 6.0 6.3 5.7 5.3 5.3 5.3 NS 7.0 7.0 7.3 6.7 7.7 6.7 6.7 6.0 6.0 7.0 6.0 6.3 6.3 6.0 6.3 6.0 6.0 5.3 5.3 4.7 5.0 1.3 7.0 7.0 6.7 7.0 7.0 7.0 6.3 6.7 7.0 6.0 6.0 6.3 5.7 5.7 6.0 5.7 5.3 5.7 5.3 5.3 4.7 P>F=0.07 1.6 7.3 6.0 6.0 7.0 6.0 5.7 6.3 6.0 5.7 5.3 6.3 5.0 6.3 6.7 5.7 6.0 5.3 6.0 6.0 5.7 5.3 NS 7.1 6.9 6.9 6.8 6.8 6.7 6.5 6.4 6.3 6.2 6.1 6.1 6.1 6.1 6.1 5.9 5.8 5.7 5.5 5.3 5.1 NS 6 .0 6.3 6 .0 1.3 * Colonial Bentgrass Color (Genetic color): 9 = dark green and 1 = light green. Green (Greenup): 9 = best and 1 = worst greenup. Leaf (Leaf texture): 9 = fine and 1 = coarse. Visual quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. NS = means are not significantly different at the 0.05 level. 21 Species and Cultivar Trials Table 2. Percentage fall living cover data1for cultivars in the 1998 Fairway Height Bentgrass Trial. Cultivar 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Percentage fall cover (%) Backspin Century Penneagle SR 1119 Trueline Providence Penn G-6 Imperial Grand Prix Seaside II PST-9HG* Penncross Princevill Tiger PST-OVN SRX IBP A A L-93 Seaside SRX 1120 GolfStar** ABT-Col-2* SRX 7MOBB* PST-9PM* ISI At-5* SR 7100* SRC 7MODD* 78.3 78.3 76.7 73.3 71.7 70.0 70.0 68.3 66.7 66.7 65.0 65.0 63.3 63.3 63.3 61.7 58.3 58.3 58.3 58.3 56.7 55.0 53.3 50.0 50.0 45.0 17.0 LSD(005) T T 71These figures represent the percentage area per plot covered by bentgrass. * Colonial Bentgrass ** Idaho Bentgrass 22 Species and Cultivar Trials Green Height Bentgrass Cultivar Trial - Established 1993 Nick E. Christians and James R. Dickson This is the final year o f data from the Green Height Bentgrass Cultivar trial established in the fall o f 1993. The area was maintained at a 3/16-inch mowing height. This was a National Turfgrass Evaluation Program (NTEP) trial and was conducted at several research stations in the U.S. It contained 28 seeded cultivars, including a number o f experimentáis. The cultivars were maintained with a fertilizer program o f 1/4 lb N applied at 14-day intervals with a total of 4 lbs o f N/1000 ft2/growing season. Fungicides were used as needed in a preventive program. Herbicides and insecticides were applied as needed. A new Green Height Bentgrass Cultivar trial was begun in September 1998. Twenty-six cultivars including numerous experimentáis are being screened. The only data collected from this study in 1998 were estimates o f the percentage o f green ground cover taken in the fall. Table 1. 1998 visual quality and other physical ratings for cultivars in the 1993 Green Height Bentgrass Trial. Quality Cultivar 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Penn G-2 (G-2) Crenshaw Imperial (Syn 92-5) Penn A -l (A -l) Penn G-6 (G-6) Penn A-4 (A-4) BAR Ws 42102 Century (Syn 92-1) Backspin (Syn 92-2) Regent Cato Southshore Providence SR 1020 Loft's L-93 (L-93) PRO/CUP Pennlinks 18th Green ISI-Ap-89150 BAR As 492 Trueline Penncross DG-P MSUEB Lopez Mariner (Syn-1-88) Tendenz* Seaside L S D ( o.o5> Color Green Leaf May June July Aug Mean 6.3 7.0 6.0 6.7 6.3 5.7 6.0 5.3 5.7 6.0 5.3 5.7 6.7 4.7 6.0 5.7 6.0 6.0 6.0 6.0 5.0 5.7 6.0 5.7 5.7 6.3 6.0 5.3 NS 6.0 6.7 6.0 6.3 6.0 5.7 5.0 5.7 6.0 5.0 6.0 5.3 5.3 5.7 5.3 5.0 5.0 6.0 5.7 5.7 4.3 5.3 5.0 5.0 4.3 5.0 5.3 4.7 1.1 7.7 6.7 7.3 7.7 6.3 7.3 6.3 7.0 7.0 6.0 6.3 6.7 6.3 7.3 6.0 5.0 6.0 6.0 5.7 5.7 6.0 5.3 5.7 6.0 5.7 6.0 5.3 5.0 7.3 7.0 7.0 6.3 7.0 6.7 6.7 6.7 6.7 6.3 6.0 6.3 5.7 6.3 6.0 6.0 6.0 5.7 5.3 5.7 5.7 5.7 6.0 5.7 5.7 5.3 5.3 5.0 NS 8.0 6.7 7.7 7.0 7.0 7.0 7.0 6.7 7.0 6.0 6.3 6.0 5.3 6.3 6.3 6.0 6.0 6.0 5.7 5.3 7.7 7.3 7.3 7.3 7.7 6.7 6.7 6.7 6.3 7.0 7.0 6.7 7.0 6.7 6.3 6.0 5.7 6.0 6.0 6.0 5.7 5.3 6.0 6.0 5.7 6.3 5.0 4.7 1.1 7.0 7.0 6.0 7.3 6.0 6.0 5.7 6.0 5.7 6.0 5.7 6.3 7.0 6.0 6.3 6.0 6.3 6.0 6.7 6.3 5.7 6.0 5.7 5.7 5.7 5.7 5.3 5.0 NS 7.5 7.0 7.0 7.0 6.9 6.6 6.5 6.5 6.4 6.3 6.3 6.3 6.3 6.3 6.3 6.0 6.0 5.9 5.9 5.8 5.8 5.8 5.8 5.8 5.8 5.8 5.1 4.9 1.0 6.0 6.0 5.3 6.0 6.0 5.7 4.7 5.0 1.4 1.0 * Colonial Bentgrass Color (Genetic color): 9 = dark green and 1 = light green. Green (Greenup): 9 = best and 1 = worst greenup. Leaf (Leaf texture): 9 = fine and 1 = coarse. Quality based on a scale of 9 to 1: 9 = best, 6 = lowest acceptable, and 1 = worst quality. NS = means are not significantly different at the 0.05 level. 23 Species and Cultivar Trials Table 2. Percentage fall living cover data1 for cultivars in the 1998 Green Height Bentgrass Trial. Cultivar 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Backspin Penn A-4 Imperial Providence ISI Ap-5 Pennlinks Penncross Penn A-2 L-93 Syn 96-2 SR 1119 ABT-CRB-1 BAR AS 8FUS2 SR 7200* Penn G-l Penn A-l Bavaria* Pick CB 13-94 SRX 1BPAA Century BAR CB 8US3 Pick MVB* Syn 96-1 SRX 1120 SRX 1NJH Crenshaw PST-A2E Penn G-6 Syn 96-3 LSD/o.05) Percentage fall cover (%) 81.7 78.3 76.7 76.7 75.0 75.0 75.0 75.0 73.3 73.3 73.3 71.7 71.7 71.7 71.7 71.7 70.0 70.0 70.0 68.3 68.3 68.3 68.3 68.3 68.3 66.7 66.7 66.7 65.0 NS ‘These figures represent the percentage area per plot covered by bentgrass. *Velvet Bentgrass 24 Species and Cultivar Trials Overseeding of Northern Turfgrass Sports Fields with Bermudagrass David D. Minner, Roch Gaussoin, and Steve Keeley Objective: To determine if seeded bermudagrass can be established in the summer and then used for intensely trafficked sport fields in late summer/early fall. Procedure: This is a cooperative research project with Dr. Roch Gaussoin, University o f Nebraska, and Dr. Steve Keeley, Kansas State University. Field plots were also established in Kansas and Nebraska, but only the Iowa data is reported at this time. This field research project was established at the Iowa State University Horticulture Research Station, Ames, IA. On 19 June 1998 the study area was prepared by treating the existing stand o f grass and weeds with glyphosate. Eleven days later all o f the vegetation was dead and approximately 50 percent o f the area was showing exposed soil. On 2 July 1998 the test area was verticut in two directions to a depth o f 0.5 inches. The area was immediately fertilized, seeded, and watered. One pound o f N, P205, and K20 was applied per 1000 sq. ft. Six bermudagrass varieties (3 lbs. seed /1000 sq. ft.) and Bright Star perennial ryegrass (15 lbs. Seed/1000 sq. ft.) were established in 8 foot by 15 foot whole-plots. Grass was watered and mowed to a two-inch cutting height. Plots were rated on 24 August and then half o f each bermudagrass whole-plot was slit seeded with Bright Star perennial ryegrass (10 lbs/1000 sq. ft.) to provide a 4-foot by 15-foot split-plot. The experimental design was a randomized complete block with seven whole-plot treatments (bermudagrasses: Sultan, Numex Sahara, SWI-10, Blend C, Mirage, and Pyramid), two split-plot treatments (with or without perennial ryegrass), and three replications. Traffic was applied with a Brouwer roller that was modified with football cleats to supply differential-slip-type traffic. Fifteen passes over the test area were made with the traffic simulator between 27 August and 15 September 1998. Table 1. Percent turf cover for six bermudagrass selections and one perennial Grass Sultan NuMex Sahara Mohawk Blend Cz Mirage Pyramid Bright Star z Blend C contains Princes 50%, SWI-10 25%, and Sultan 25%. Avg94.6 94.6 97.6 93.3 95.0 96.3 23.3 Table 2. Turfgrass color, quality, and cover average ratings of 16 Oct 1998. Grass Sultan Sultan +P R NuMex Sahara NuMex Sahara + PR Mohawk Mohawk + PR Blend C Blend C + PR Mirage Mirage + PR Pyramid Pyramid + PR Bright Star PR Color 3 4 3 4 3 4 3 4 3 4 3 4 8 Quality 6 6 6 6 6 6 6 6 6 6 6 6 5 25 Cover 75 73 73 72 75 73 73 73 72 73 72 75 52 Species and Cultivar Trials Results: Table 1 shows the advantage o f establishing a warm season grass like bermudagrass during the summer compared with perennial ryegrass. On 24 August all o f the bermudagrass selections had greater than 90% turf cover, while perennial ryegrass had only 23% turf cover. This resulted in a substantial advantage that would coincide with the beginning o f a normal fall football season. Even with an additional perennial ryegrass seeding on 24 August, the bermudagrasses provided more turf cover than perennial ryegrass at the end o f the football season. Bermudagrass cover was approximately 75% compared with 52% for perennial ryegrass (Table 2). All o f the bermudagrass cultivars retained 100% o f their green color on 15 September. By 1 October the bermudagrasses were showing signs o f winter dormancy and green color retention was approximately 60 percent. By 16 October 1998 bermudagrass leaves were mostly brown with only about 30% green tissue present. Table 2 shows that there was a slight improvement in turf color on 16 October when bermudagrass was overseeded with perennial ryegrass. By 16 October the perennial ryegrass only treatment had much better color than the bermudagrass or the bermudagrass/overseeded with rye treatments. 26 Herbicide and Growth Regulator Studies Preemergent Annual Grass Control Study Barbara R. Bingaman, Melissa C. McDade, Michael B. Faust, and Nick E. Christians In this study various preemergent herbicide and herbicide plus fertilizer formulations were screened for efficacy in controlling annual grass weed species in turfgrass. The study was conducted at the Iowa State University Horticulture Research Station near Ames, IA in established 'Nassau' Kentucky bluegrass that had a heavy crabgrass infestation in 1997. The soil was a Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll) with an organic matter content o f 3.35%, a pH o f 6.85, 5 ppm P, and 93 ppm K. The study was arranged as a randomized complete block. Individual plot size was 5 x 5 ft with three replications. Thirty-three treatments were included in single and split applications with a fertilized control and an untreated control (Table 1). Preemergent applications were made on April 29, 1998 before crabgrass germination. It was 54° F with overcast skies and a northeast wind at 10 mph. Crabgrass was detected in the untreated controls on June 10. Sequential (POST) applications o f treatments 2 - 9 were made on June 16 (6 to 8 weeks after initial applications). Temperatures were in the mid 70's and it was mostly sunny with a light NW wind. The crabgrass plants in the untreated controls were in the 1- to 2-leaf stage. On July 1, sequential applications o f treatments 10-15 and 21-25 were made. It was 80° F and mostly sunny with northwest winds at 5-10 mph. Crabgrass in the untreated controls was in the 1- to 4-leaf stage. Liquid formulations were applied at 30 psi with a carbon dioxide backpack sprayer equipped with TeeJet® #8006 flat fan nozzles. Liquids were diluted into 287 ml o f water which translates to an application rate o f three gal/1000 ft2. Granular materials were applied using ‘shaker dispensers’. Visual turf quality data were taken weekly from May 5 through September 2 (Tables 2 and 3). Quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. The plot was checked for phytotoxicity on each o f these dates and none was detected. Annual grass populations were estimated as percentage crabgrass cover per plot. These data were taken weekly from July 7 through September 2 (Table 4). Annual grass control was determined by calculating percentage reductions in crabgrass cover as compared with the untreated controls (Table 5). Broadleaf population data for oxalis, clover, dandelion, and black medic were taken on September 2 (Table 6). All data were analyzed using the Statistical Analysis System (SAS, Version 6.12) and the Analysis o f Variance (ANOVA) procedure. Fisher's Least Significant Difference (LSD) tests were used to compare means separation. The quality o f turf treated with herbicide plus fertilizer formulations was better than the untreated controls on May 5 (Table 2). By June 4, most o f the fertilizer effects on quality were gone. Improved quality also was detected in turf receiving sequential applications o f herbicide plus fertilizer formulations on either June 16 or July 1. Nineteen o f the materials produced significantly better mean quality than the untreated controls (Table 3). There were significant differences in percentage crabgrass cover on all data collection dates (Table 4). Seventeen of the materials maintained percentage crabgrass cover < 5.3% for the duration o f the study and twenty materials suppressed cover to < 10% for this period. Twenty-four herbicide formulations produced mean percentage cover < 8.7%. Crabgrass cover reductions were significantly different on all collection dates (Table 5). Twenty herbicides reduced crabgrass cover by > 95% as compared with the untreated controls on July 7 and 10 herbicides provided 100% control on that date. By July 29, the level o f control had decreased for some o f the formulations but 21 herbicides were still providing > 90% control. By September 2, 16 herbicides were still reducing crabgrass cover > 90% as compared with the untreated controls. Thirteen herbicides provided > 90.0% crabgrass control for the duration o f the study (Table 5). Dandelion was the only broadleaf weed species that was distributed among all o f the plots (Table 6). There were significantly fewer dandelions in turf treated with eight o f the herbicides as compared with the untreated controls. Some o f the largest dandelion populations were found in turf treated with herbicides that provided excellent control 27 Herbicide and Growth Regulator Studies o f crabgrass. In these areas, dandelions could more easily establish because o f less competition from crabgrass and other weed species. Oxalis, black medic, and clover distributions were sporadic. The efficacy o f the tested herbicides in controlling these species, therefore, could not be determined from these data. Table 1. Materials and rates for the 1998 Pre- & Postemergent Annual Weed Control Study. Material 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. Untreated control S-80961 S-80961 S-80971 S-80971 S-81621 S-81621 S-81611 S -81611 Team Pro NAF-324 0.86GR2 Team Pro NAF-324 0.86GR2 Pendimethalin 0.86GR2 Pendimethalin 0.86GR2 Dimension 0.10 GR2 Barricade 0.22 GR2 (PRE) Rate (g product/ft2) N /A 2.06 2.06 2.06 2.06 1.56 1.56 1.53 1.53 (PRE) Rate lb a.i./A 1.5 2.0 1.5 2.0 0.38 0.5 Rate lb a.i./A (POST) Rate (g product/ft2) N /A none 2.06 none 2.06 none 1.56 none 1.53 (POST) Rate lb a.i./A 1.5 none 1.5 none none none Timing of application PRE 0.75 BAS051434H Drive 75DF3 PRE 0.50 BAS051434H Drive 75DF3 PRE 0.75 BAS 514 0.57GR3 PRE 0.50 BAS 514 0.57GR3 PRE 3.00 Pendimethalin 60WDG3* POST 0.75 BAS051434H Drive 75DF 1.0% + M S03 POST 0.50 BAS051434H Drive 75DF 22. 1.0% + M S03 POST 0.75 BAS 514 0.57GR3 23. POST 0.50 24. BAS 514 0.57GR3 POST 0.50 Dimension 1EC 25. 0.50% + NIS3 PRE 0.25 26. Dimension 1EC4 PRE 0.25 Dimension 0.164FG AND4454 27. PRE 0.25 28. Dithiopyr 1.06XF 98-0254 PRE 0.25 29. Dithiopyr 0.89XF 98-0264 PRE 0.25 30. Dithiopyr 2.20XF 98-0294 PRE 0.25 Dimension 40WP4 31. PRE N /A 32. Fertilized control4 ‘These materials are being screened for The Scotts Company. 2These materials are being screened for Dow AgriSciences. 3These materials are being screened for BASF. 4These materials are being screened for Rohm and Haas and were applied with methylene urea (39-0-0) at 4 lb/1000 ft2. Initial applications (PRE) were made on April 29, Scott's (trt 2-9) sequential applications at 6 to 8 WAT on June 16, Dow (trt 10-15) sequential applications at 8 to 10 WAT on July 1, and BASF (trt 21-25) POST applications on July 1. 16. 17. 18. 19. 20. 21. 28 Herbicide and Growth Regulator Studies Material 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. ----ITT---- Untreated control S-8096 S-8096 S-8097 S-8097 S-8162 S-8162 S-8161 S-8161 Team Pro NAF-324 0.86GR Team Pro NAF-324 0.86GR Pendimethalin 0.86GR Pendimethalin 0.86GR Dimension 0.10 GR Barricade 0.22 GR BAS051434H Drive 75DF BAS051434H Drive 75DF BAS 514 0.57GR BAS 514 0.57GR Pendimethalin 60WDG BAS051434H Drive 75DF BAS051434H Drive 75DF BAS 514 0.57GR BAS 514 0.57GR Dimension 1EC + NIS Dimension 1EC Dimension 0.164FG AND445 Dithiopyr 1.06XF 98-025 Dithiopyr 0.89XF 98-026 Dithiopyr 2.20XF 98-029 Dimension 40WP Fertilized control May 5 7.0 8.7 8.7 8.3 8.3 7.3 8.0 7.0 8.7 7.3 9.0 8.7 8.0 8.3 9.0 7.3 7.7 8.0 8.0 8.0 7.7 7.3 8.0 7.3 7.0 8.3 7.7 8.7 8.0 7.7 7.3 8.0 1.0 L S D oo5 May 13 7.0 7.0 7.0 7.3 7.7 6.7 6.7 7.0 7.7 7.7 7.0 6.7 6.7 7.0 7.0 6.0 6.3 7.0 6.7 6.7 7.0 6.7 6.7 7.0 7.0 7.7 7.3 7.0 7.0 7.3 7.7 7.0 NS May 19 6.3 7.7 7.3 8.0 8.0 6.3 6.3 7.3 7.0 7.7 7.7 7.7 8.0 8.0 7.0 5.7 5.7 6.0 6.0 6.0 6.3 6.3 6.3 6.0 6.0 7.3 7.3 7.0 7.0 7.7 7.3 7.0 1.1 May 27 6.0 7.7 7.7 7.7 9.0 7.0 6.7 8.0 7.0 7.7 7.7 8.3 8.7 8.3 7.0 5.3 5.7 5.7 6.0 6.7 6.7 6.0 6.0 6.3 6.0 9.0 8.3 8.7 8.0 8.3 8.3 8.3 1.0 June 4 6.0 6.3 6.0 6.7 6.7 6.3 6.0 6.7 6.3 6.7 6.0 6.7 6.0 6.7 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.3 6.3 6.0 6.0 6.3 7.0 6.3 6.0 6.3 7.0 6.7 0.6 June 10 5.3 6.7 6.7 7.0 7.0 5.7 5.7 6.0 6.3 6.0 6.0 6.7 7.06.7 6.3 5.0 5.0 5.0 5.3 5.3 5.3 5.3 5.0 5.0 5.3 7.7 7.3 7.3 7.0 7.7 7.3 8.3 1.1 June 16 5.7 7.3 7.7 7.3 7.3 6.7 7.3 6.3 6.3 7.3 7.0 7.3 7.7 7.7 7.3 5.0 5.7 5.7 6.3 6.3 6.3 6.3 5.7 5.0 5.7 9.0 8.3 7.7 8.7 8.3 8.0 8.3 1.4 June 24 6.0 6.0 8.0 6.0 8.0 6.0 8.0 6.0 8.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 - June 30 6.0 6.7 9.0 6.3 8.7 6.0 8.0 6.3 8.0 6.3 6.3 6.3 7.0 6.7 6.3 5.7 6.3 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 7.3 7.3 7.7 8.0 7.7 7.0 7.3 0.9 July 7 6.0 6.3 8.7 7.3 8.0 6.0 6.7 6.7 7.0 8.7 6.3 8.3 6.3 6.3 6.0 6.3 6.0 6.0 6.0 6.0 6.3 6.0 6.3 6.0 6.0 6.7 7.3 7.0 7.0 7.0 7.7 6.3 1.1 Initial applications (PRE) were made on April 29, Scott's (trt 2-9) sequential applications at 6 to 8 WAT on June 16, Dow (trt 10-15) sequential applications at 8 to 10 WAT on July 1, and BASF (trt 21-25) POST applications on July 1. NS = means are not significant at the 0.05 level. -- = means comparisons tests are not valid for these data. Material 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. — ITT---- Untreated control S-8096 S-8096 S-8097 S-8097 S-8162 S-8162 S-8161 S-8161 Team Pro NAF-324 0.86GR Team Pro NAF-324 0.86GR Pendimethalin 0.86GR Pendimethalin 0.86GR Dimension 0.10 GR Barricade 0.22 GR BAS051434H Drive 75DF BAS051434H Drive 75DF BAS 514 0.57GR BAS 514 0.57GR Pendimethalin 60WDG BAS051434H Drive 75DF BAS051434H Drive 75DF BAS 514 0.57GR BAS 514 0.57GR Dimension 1EC + NIS Dimension 1EC Dimension 0.164FG AND445 Dithiopyr 1.06XF 98-025 Dithiopyr 0.89XF 98-026 Dithiopyr 2.20XF 98-029 Dimension 40WP Fertilized control L S D o.<)5 July 14 6.0 6.0 7.0 6.3 6.7 6.3 6.3 6.3 6.7 7.0 6.3 7.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.3 6.3 6.0 6.0 6.0 6.0 6.7 6.3 6.7 7.0 6.7 6.7 6.7 0.7 July 29 7.0 7.3 7.3 7.0 7.3 7.0 7.0 7.0 7.0 7.7 7.0 7.3 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.3 7.3 7.0 NS Aug 4 7.0 7.0 7.3 7.0 7.3 7.0 7.0 7.0 7.3 8.0 7.0 7.7 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.7 7.3 7.3 0.5 Aug 11 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 - Aug 18 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 - Aug 27 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 7.0 - Sept 2 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 6.0 - 2k Mean 6.4 6.9 7.4 7.0 7.5 6.5 6.9 6.7 7.1 7.1 6.8 7.2 7.0 7.0 6.8 6.2 6.3 6.4 6.4 6.5 6.5 6.4 6.4 6.3 6.4 7.2 7.1 7.1 7.1 7.2 7.2 7.1 0.3 Initial applications (PRE) were made on April 29, Scott's (trt 2-9) sequential applications at 6 to 8 WAT on June 16, Dow (trt 10-15) sequential applications at 8 to 10 WAT on July 1, and BASF (trt 21-25) POST applications on July 1. NS = means are not significant at the 0.05 level. -- = means comparisons tests are not valid for these data. 29 Herbicide and Growth Regulator Studies T a b le 4. Percentage crabgrass cover1 in Kentucky bluegrass treated for the 1998 Pre- & Postem ergent Annual W eed Control Study. A u gl 1 Aug 18 Aug 27 Material July 7 July 14 July 24 July 29 Aug 4 Sept2 Mean 38.3 2.3 0.3 3.3 53.3 3.7 0.3 3.7 29.5 1.7 1.0 1.0 0.4 2.2 0.4 0.4 0.9 0.7 1.3 5.6 4.3 0.8 1.7 20.6 23.3 12.7 28.3 1.7 0.9 1.7 3.9 14.3 2.9 4.5 1.2 11.7 7.1 8.7 4.0 17.6 9.0 % 1 . 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. Untreated control S-8096 S-8096 S-8097 S-8097 S-8162 S-8162 S-8161 S-8161 Team Pro NAF-324 0.86GR Team Pro NAF-324 0.86GR Pendimethalin 0.86GR Pendimethalin 0.86GR Dimension 0.10 GR Barricade 0.22 GR BAS051434H Drive 75DF BAS051434H Drive 75DF BAS 514 0.57GR BAS 514 0.57GR Pendimethalin 60WDG BAS051434H Drive 75DF BAS051434H Drive 75DF BAS 514 0.57GR BAS 514 0.57GR Dimension 1EC + NIS Dimension 1EC Dimension 0.164FG AND445 Dithiopyr 1.06XF 98-025 Dithiopyr 0.89XF 98-026 Dithiopyr 2.20XF 98-029 Dimension 40WP Fertilized control L S D ().o 5 13.3 0.7 14.0 0.7 26.7 1 .0 26.7 0.3 33.3 2.0 25.0 2.7 35.0 2.3 0.3 0.0 0.0 0 .0 0 .0 0 .0 0 .0 0.0 0.0 0.7 0.3 0.3 0.0 0 .0 0.0 1.7 0.3 0.7 0.3 0.3 0.3 3.7 5.0 0.0 0.7 0.7 2.0 0.3 0 .0 1.0 0.0 0 .0 0.3 0 .0 0.0 0 .0 0 .0 0.0 0 .0 0 .0 0 .0 0 .0 0.7 0.7 2.0 0.7 0.3 1.0 0.3 0.7 0.3 0 .0 0 .0 1.0 1.0 1.0 1.7 0.3 0.3 0.3 9.0 11.7 2.0 11.7 2.0 1.3 0.3 8.3 2.7 6.7 1.3 5.0 5.3 3.3 4.3 0 .0 0 .0 0 .0 0 .0 1.0 2.7 25.7 21.7 10.3 25.0 1.7 0.7 0.3 3.3 10.0 3.0 4.3 1.3 18.3 18.3 10.7 28.3 1.3 1.0 0 .0 0.7 4.0 13.3 7.0 9.3 0.3 0 .0 0 .0 0 .0 0 .0 0.3 3.0 2.3 0.7 0.3 6.7 2.0 0.7 6.3 2.3 1.0 0.7 5.3 5.8 1 .0 0 .0 6.7 3.7 1.3 1.7 6.0 6.9 1.7 0 .0 1.0 7.0 7.7 8.7 3.7 20.0 10.1 0.3 1.0 16.7 23.3 13.7 28.3 3.3 21.7 20.0 9.3 23.3 0.7 1 .0 0 .0 0 .0 0 .0 2.0 3.7 11.7 3.7 2.3 1.7 12.0 8.7 12.0 4.0 16.7 11.8 3.0 9.3 2.0 2.3 0.7 12.0 4.3 6.7 '2.3 13.3 8.7 0.7 8.3 1 .0 1.7 0.7 16.7 7.3 5.7 3.7 16.7 9.3 1.0 0 .0 0.7 1.7 0.7 2.7 6.7 6.7 1.7 2.0 20.0 25.0 13.3 30.0 2.0 1.3 4.3 6.7 18.3 5.3 6.3 2.0 10.3 6.3 11.7 5.3 18.3 9.8 1 .0 0.7 0.7 2.3 5.0 8.3 2.0 2.3 33.3 35.0 21.7 41.7 2.0 2.3 4.0 7.0 26.7 2.3 10.0 0.7 13.7 10.3 13.3 6.7 30.0 14.1 5.3 1.7 2.0 1.7 1.7 3.7 12.0 8.3 2.0 3.7 36.7 41.7 26.7 56.7 3.7 2.3 4.0 10.0 35.0 3.7 12.0 3.7 20.0 13.3 18.3 8.3 31.7 16.0 0 .1 1.0 'These figures represent the area per plot covered by crabgrass. Initial applications (PRE) were made on April 29, Scott's (trt 2-9) sequential applications at 6 to 8 W AT on June 16, Dow (trt 10-15) sequential applications at 8 to 10 W AT on July 1, and BASF (trt 21-25) POST applications on July 1. Table 5. Percentage crabgrass cover reductions' in Kentucky bluegrass treated for the 1998 Pre- & Postemergent Annual Weed Control Study. Aug 11 Aug 18 Aug 27 Aug 4 Sept 2 July 14 July 24 July 29 Material July 7 1. Untreated control S-8096 S-8096 S-8097 S-8097 S-8162 S-8162 S-8161 S-8161 Team Pro NAF-324 0.86GR Team Pro NAF-324 0.86GR Pendimethalin 0.86GR Pendimethalin 0.86GR Dimension 0.10 GR Barricade 0.22 GR BAS051434H Drive 75DF BAS051434H Drive 75DF BAS 514 0.57GR BAS 514 0.57GR Pendimethalin 60WDG BAS051434H Drive 75DF BAS051434H Drive 75DF BAS 514 0.57GR BAS 514 0.57GR Dimension 1EC + NIS Dimension 1EC Dimension 0.164FG AND445 Dithiopyr 1.06XF 98-025 Dithiopyr 0.89XF 98-026 Dithiopyr 2.20XF 98-029 Dimension 40WP Fertilized control 0 .0 0 .0 0 .0 0 .0 95.0 95.2 96.3 98.8 100.0 100.0 100.0 100.0 100.0 98.8 100.0 97.5 100.0 100.0 100.0 97.5 97.6 93.8 93.8 100.0 100.0 100.0 100.0 98.8 100.0 100.0 100.0 100.0 97.5 100.0 95.2 97.5 97.6 97.5 100.0 92.5 100.0 100.0 98.8 96.3 85.7 68.8 75.0 87.5 90.5 90.0 95.0 97.5 100.0 100.0 100.0 97.5 95.2 90.0 95.0 97.5 71.4 3.9 31.3 32.3 4.8 12.3 18.9 31.3 50.0 60.0 85.0 61.3 0 .0 33.3 6.4 12.3 100.0 97.6 95.0 93.8 100.0 100.0 97.5 96.3 100.0 100.0 98.8 100.0 95.2 97.5 97.5 87.5 54.8 62.5 68.8 77.4 83.3 82.5 88.8 96.3 95.0 92.9 83.8 93.8 100.0 97.5 97.5 96.3 52.4 37.6 50.0 73.8 85.0 73.8 71.3 72.5 92.5 90.5 67.5 78.8 88.1 86.3 95.0 86.3 57.1 37.6 59.9 25.1 49.1 43.5 37.8 35.0 L S D 5 ‘These values represent reductions in percentage crabgrass cover per plot as compared with Initial applications (PRE) were made on April 29, Scott's (trt 2-9) sequential applications at 8 to 10 W AT on July 1, and BASF (trt 21-25) POST applications on July 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. 25. 26. 27. 28. 29. 30. 31. 32. o o 30 0 .0 94.0 100.0 98.0 98.0 94.0 99.0 100.0 98.0 99.0 97.0 85.0 84.0 100.0 97.0 50.0 30.0 59.0 14.9 90.0 100.0 94.0 89.0 65.0 89.0 93.0 95.0 64.0 74.0 64.0 88.0 50.0 35.5 0.0 89.3 100.0 97.3 98.7 98.7 100.0 100.0 96.0 98.7 96.0 86.7 82.7 96.0 96.0 13.3 20.0 62.7 6.7 97.3 100.0 96.0 88.0 62.7 92.0 90.7 97.3 52.0 82.7 .73.3 90.7 46.7 34.6 0.0 93.3 99.0 100.0 99.0 89.5 97.1 98.1 95.2 98.1 92.4 81.0 81.0 95.2 94.3 42.9 28.6 61.9 14.3 94.3 96.2 87.6 81.0 47.6 84.8 81.9 94.3 70.5 81.9 66.7 84.8 47.6 28.0 0 .0 93.9 99.1 91.3 97.4 86.9 100.0 97.4 98.3 98.3 93.9 86.9 78.2 94.8 93.9 13.0 8.6 43.4 Mean 0 .0 0 .0 93.1 99.4 93.1 98.1 90.0 96.9 96.2 96.9 96.9 93.1 77.5 84.4 96.2 93.1 31.2 21.8 50.0 94.3 99.7 97.3 98.7 93.8 99.1 99.1 97.2 97.6 96.4 81.6 87.0 97.8 94.7 32.1 19.6 59.2 7.4 95.1 97.7 95.4 88.7 55.9 89.3 86.9 96.6 58.5 76.6 73.8 87.3 42.9 31.8 0 .0 0 .0 94.8 93.9 89.6 81.7 30.4 93.9 73.9 98.3 64.3 73.0 65.2 82.6 21.7 36.7 93.1 95.6 92.5 81.2 34.3 93.1 77.5 93.1 62.5 75.0 65.6 84.4 40.6 30.0 the untreated controls. 6 to 8 W AT on June 16, Dow (trt 10-15) sequential applications at Herbicide and Growth Regulator Studies Table 6. The number of dandelions, oxalis, black medic, and percentage clover cover1 in Kentucky bluegrass treated for the ________ 1998 Pre- & Postemergent Annual Weed Control Study (data taken September 2).___________________________ Percentage Counts cover M aterial Clover D andelion Oxalis Black Medic 1. Untreated control 25.3 0.7 0.0 6.7 2. S-8096 19.7 0.3 0.3 1.7 0.0 0.7 3. S-8096 6.3 8.3 4. S-8097 26.3 0.0 6.3 1.7 5. S-8097 6.0 0.7 1.7 0.0 6. S-8162 15.7 0.0 1.7 1.7 7. S-8162 0.0 12.3 0.0 1.7 8. S-8161 20.0 0.7 1.7 5.0 9. S-8161 14.0 0.3 1.0 1.7 10. Team Pro NAF-324 0.86GR 21.0 0.0 0.0 0.3 11. Team Pro NAF-324 0.86GR 0.0 0.0 15.3 3.3 12. Pendimethalin 0.86GR 14.0 0.0 1.0 8.7 13. Pendimethalin 0.86GR 0.0 0.0 15.3 6.7 14. Dimension 0.10 GR 12.7 0.0 1.0 0.0 15. Barricade 0.22 GR 16.7 0.0 0.0 0.0 16. BAS051434H Drive 75DF 15.0 2.3 0.0 0.3 17. BAS051434H Drive 75DF 0.7 21.3 0.0 0.3 18. BAS 514 0.57GR 16.7 0.7 0.0 0.0 19. BAS 514 0.57GR 19.7 0.7 2.7 0.0 20. Pendimethalin 60WDG 13.3 0.3 0.3 5.0 21. BAS051434H Drive 75DF + MSO 1.7 1.0 0.0 0.0 22. BAS051434H Drive 75DF + MSO 3.0 2.0 0.0 0.3 23. BAS 514 0.57GR 1.7 0.7 1.0 0.0 24. BAS 514 0.57GR 0.3 8.3 0.3 0.3 25. Dimension 1EC + NIS 14.3 0.3 0.7 16.7 26. Dimension 1EC 17.3 0.0 0.0 5.0 27. Dimension 0.164FG AND445 24.7 0.3 2.0 0.0 28. Dithiopyr 1.06XF 98-025 12.7 0.0 0.3 0.3 29. Dithiopyr 0.89XF 98-026 14.0 0.0 0.0 10.0 30. Dithiopyr 2.20XF 98-029 18.0 0.0 0.3 13.3 31. Dimension 40WP 17.7 0.3 0.3 0.0 32. Fertilized control 12.0 0.0 0.0 10.3 12.6 NS 2.6 NS L S D o os ^ h e s e values represent the number of dandelion, black medic, and oxalis plants per plot. Percentage clover cover data represent the area covered by clover per plot. Initial applications (PRE) were made on April 29, Scott's (trt 2-9) sequential applications at 6 to 8 WAT on June 16, Dow (trt 10-15) sequential applications at 8 to 10 WAT on July 1, and BASF (trt 21-25) POST applications on July 1. NS = means are not significantly different at the 0.05 level. 31 Herbicide and Growth Regulator Studies Postemergent Annual Weed Control Study B arbara R. Bingaman, M elissa C. M cDade, an d N ick E. Christians The efficacy o f experimental postemergence herbicides for the control o f annual grass and broadleaf weed species in turf was evaluated in this study. It was conducted at the Iowa State University Horticulture Research Station north o f Ames, IA in an area of'common' Kentucky bluegrass. The soil was a Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll) with 3.4% organic matter, 38 ppm P, 145 ppm K, and a pH o f 7.0. Turf mowing height was consistent with a high (home lawn) mowing regime o f 2 to 3” for Kentucky bluegrass. The experiment was arranged in a randomized complete block design. Individual plots were 5 x 5 ft with 3 replications. There were fourteen treatments including an untreated control (Table 1). Treatments were applied early postemergence (EARLY POST) when crabgrass was in the 1- to 4-leaf stage and mid-postemergence (MID POST) when crabgrass had 1 to 3 tillers. EARLY POST treatments included Dimension 1EC at 1.5 oz/1000 ft2, Acclaim Extra 0.57EW at 0.06 lb a.i./lOOO ft2 and Preclaim 3.09EC at 1.5 and 2.0 lb a.i./lOOO ft2 Preclaim 3.09EC also was applied MID POST at 2.0 and 3.0 lb a.i./lOOO ft2. Bas514 50WP was applied MID POST at 0.55 oz/1000 ft. Experimental formulations L-0051, L-0260, L-0289, L-0259, an L-0261 were applied MID POST at 3.0 lb a.i./lOOO ft2. In addition, L-0051 was applied MID POST at 4.0 lb a.i./1000 ft2. Early postemergence applications were made on July 1, 1998 and the MID POST on July 14. Liquids were applied using a carbon dioxide backpack sprayer equipped with Teejet® #8006 flat fan nozzles at 30 psi. The herbicides were diluted into 283 ml o f water that translates to an application volume o f 3 gal/1000 ft2. Kentucky bluegrass phytotoxicity was evaluated weekly beginning July 9. Phytotoxicity was rated using a 9 to 1 scale with 9 = no damage, 6 = some symptoms and 1 = dead turf. Turf quality was determined weekly beginning July 9. Quality was rated visually using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. Crabgrass damage was assessed on July 29, August 4, and August 11 by estimating the percentage living crabgrass per plot (Table 2). Damage was determined by estimating the amount o f damage to the crabgrass plants within each plot using a 0 to 100% scale with 0 = no damage and 100% = dead plants. To assess crabgrass mortality, the percentage o f area per plot covered by crabgrass was estimated on July 9, August 18, August 26, September 4, September 9, September 16, and September 21 using a 0 to 100% scale (Table 3). Percentage reductions in cover were calculated by comparing the cover in treated turf to the untreated controls (Table 4). Broadleaf weed control was determined through population counts on July 21. The number o f oxalis, spurge, and dandelion plants per plot was counted. Clover populations were determined by estimating the percentage o f area per plot covered by clover (Table 5). In addition, because o f the large numbers o f small plants in some plots, percentage cover data also were taken for dandelion. All data were analyzed using the Statistical Analysis System (SAS, version 6.12) and the Analysis o f Variance (ANOVA) procedure. Differences among the means were examined with Fisher's Least Significant Difference (LSD) means separation test. There was no bluegrass phytotoxicity detected following either the EARLY POST or MID POST applications. There was a slight yellow tint observed on bluegrass following treatment with Preclaim 3.09EC but this discoloration disappeared after 24 hours. There were no visual quality differences among the treated and untreated plots throughout the season. On July 9, virtually all o f the crabgrass was dead in turf treated with the EARLY POST materials (Table 3 and 4). The MID POST treatments began to cause damage on July 17, three days after application. Symptoms included curling o f leaves, collapsed plants, and leaf discoloration. Two weeks after the MID POST applications, crabgrass plants were exhibiting a wide range o f damage from slightly damaged to moderately damaged (Table 2). Damage was severe in most treated plots by August 11. 32 Herbicide and Growth Regulator Studies There were significant differences in percentage crabgrass cover for August 18 through September 21 (Table 3). All materials significantly reduced crabgrass cover as compared with the controls on August 18, September 4, and September 9 (Table 4). On August 18, there was at least 55% crabgrass cover reduction in turf treated with the MID POST materials as compared with the untreated controls. Turf treated with the EARLY POST materials had > 93% reductions in crabgrass cover as compared with the controls on August 18. After September 9, some o f the materials were no longer providing significant reductions in crabgrass cover as compared with the untreated controls. The EARLY POST materials (treatments 9-11 and 14), however, provided > 81% reductions in crabgrass for the duration o f the study. The mean crabgrass reduction data show that all materials significantly reduced crabgrass cover as compared with the untreated controls. Mean reductions were > 50% for all materials. Two EARLY POST materials, Dimension 1EC at 1.5 oz/1000 ft2 and Preclaim 3.09EC at 2,0 lb a.i./A, provided reductions in crabgrass cover > 96%. The other EARLY POST materials (Preclaim 3.09EC at 1.5 lb a.i./A and Acclaim Extra 0.57EW at 0.06 lb a.i./A) and Preclaim 3.09EC at 2.0 and 3.0 lb a.i./A MID POST produced mean reductions > 86%. This study did not produce any hard evidence concerning the efficacy o f these materials for controlling broadleaf weeds. There were numerical differences in oxalis and spurge populations found in the treated and untreated turf but the differences were not statistically different (Table 5). Most treated turf contained more oxalis plants that the untreated controls. Clover was found only in turf treated with either the EARLY POST materials or Preclaim MID POST. These results can probably be attributed to the decrease in competition in turf where the crabgrass was killed. Dandelion counts were significantly different but no product significantly reduced dandelion populations as compared with the untreated control (Table 5). The largest dandelion populations were found in treated turf where the removal o f crabgrass had reduced competition as compared to the untreated turf. Table 1. Materials used in the 1998 Postemergence Annual Weed Study. Rate lb/1000 ft2 N/A 3.00 4.00 3.00 3.00 3.00 3.00 0.55 oz 1.50 oz Rate lb a.i./A 1.50 2.00 2.00 3.00 0.06 Materials 1 2 3 4 5 6 7 8 9 Untreated control L-00511 L-00511 L-02601 L-02891 L-02591 L-02611 BAS514 50W P1 Dimension 1EC1 Timing o f applications N/A MID POST MID POST MID POST MID POST MID POST MID POST MID POST EARLY POST 10 Preclaim 3.09 EC2 EARLY POST EARLY POST 11 Preclaim 3.09 EC2 12 Preclaim 3.09 EC2 MID POST 13 Preclaim 3.09 EC2 MID POST 14 Acclaim Extra 0.57EW 1&2 EARLY POST These materials are being screened for LESCO1 and AgrEvo2. Early post applications were made on July 1 when the crabgrass was in the 1 to 4 leaf stage and mid post applications on July 14 when the crabgrass had 1 to 3 tillers. 33 Herbicide and Growth Regulator Studies Table 2. Percentage living crabgrass per plot1 in Kentucky bluegrass treated for the 1998 Postemergence Annual _________ Weed Study.___________________________________________________________________________ Materials ___ July 29 August 4 August 11 Mean % Untreated control 100.0 45.0 L-0051 L-0051 63.3 76.7 L-0260 L-0289 66.7 L-0259 85.0 53.3 L-0261 BAS514 50WP 53.3 Dimension 1EC 0.0 Preclaim 3.09 EC 0.0 0.0 Preclaim 3.09 EC Preclaim 3.09 EC 16.7 Preclaim 3.09 EC 36.7 Acclaim Extra 0.57EW 0.0 42.2 LSDo os 'These figures represent the percentage of crabgrass per plot that was 1 2 3 4 5 6 7 8 9 10 11 12 13 14 80.0 38.3 25.0 41.7 58.3 11.7 58.3 18.3 10.0 68.3 68.3 16.7 60.0 13.7 61.7 3.7 30.0 0.0 66.7 6.7 0.0 0.0 8.3 2.0 8.3 1.7 0.0 1.0 48.0 17.3 green and healthy. 72.8 37.2 44.4 51.1 48.3 56.7 42.3 40.0 10.0 24.4 0.0 9.0 15.6 0.3 28.2 Table 3. Percentage crabgrass cover1 in Kentucky bluegrass treated for the 1998 Postemergence Annual Weed Study. Materials July 9 August 18 August 26 Sept 4 Sept 9 Sept 16 Sept 21 58.3 23.3 16.7 12.0 23.3 25.0 13.7 23.7 0.3 8.3 0.3 6.7 5.3 8.3 27.0 70.0 35.0 43.3 43.3 33.3 35.0 28.3 25.0 5.0 13.3 3.3 1.7 10.0 13.3 32.4 76.7 41.7 61.7 43.3 53.3 55.0 53.3 26.7 5.0 2.0 3.7 16.7 5.0 20.0 43.1 Mean cover 0/n 1 2 3 4 5 6 7 8 9 10 11 12 13 14 9.3 71.7 58.3 Untreated control 12.0 32.0 L-0051 33.7 25.0 26.7 25.0 L-0051 13.7 31.7 31.7 L-0260 7.7 16.7 25.0 L-0289 11.7 16.7 18.3 L-0259 20.0 L-0261 19.3 21.7 25.0 BAS514 50WP 18.3 23.3 0.0 2.0 Dimension 1EC 1.7 0.0 5.0 Preclaim 3.09 EC 8.3 Preclaim 3.09 EC 0.0 0.7 2.0 Preclaim 3.09 EC 17.3 3.7 8.3 Preclaim 3.09 EC 32.3 3.3 3.7 2.0 Acclaim Extra 0.57EW 0.3 5.3 NS 27.2 27.9 LSD 0 0 5 'These figures represent the area per plot covered by crabgrass. EARLY POST applications were made on July 1 and MID POST 34 66.7 32.0 18.3 7.0 20.0 25.3 23.7 28.3 0.7 6.7 0.3 8.7 3.7 5.0 29.8 applications on July 14. 58.7 30.0 31.0 26.1 25.6 26.7 25.7 24.3 2.1 6.2 1.5 9.0 9.0 7.8 26.8 Herbicide and Growth Regulator Studies Table 4. Percentage crabgrass cover reductions1 in Kentucky bluegrass treated for the 1998 Postemergence Annual Weed Study. Materials July 9 August 18 August 26 Sept 4 Sept 9 Sept 16 Sept 21 Mean reduction2 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 55.4 52.0 60.0 42.3 50.0 45.7 50.8 0.0 62.8 71.4 57.1 72.5 38.1 19.6 52.3 0.0 55.8 45.7 89.5 79.4 38.1 43.5 57.9 17.9 76.7 70.0 60.0 52.4 30.4 57.1 57.2 0.0 76.7 68.6 62.0 57.1 50.0 28.3 56.3 0.0 72.1 62.9 64.5 76.6 59.5 30.4 60.0 0.0 67.4 59.4 57.1 57.5 65.2 62.1 64.3 100.0 97.2 97.1 99.0 99.4 92.9 93.5 96.4 100.0 93.0 85.7 90.0 85.7 97.4 81.0 89.4 100.0 99.1 99.4 96.6 99.5 95.2 95.2 97.5 0.0 94.9 85.7 87.0 88.6 97.6 78.3 88.6 0.0 95.3 93.7 94.5 90.9 85.7 93.5 92.3 96.4 97.2 85.7 90.9 92.5 81.0 73.9 86.8 NS 37.9 46.2 47.9 44.6 46.3 56.2 42.7 L S D o os ‘These figures represent reductions in crabgrass cover in treated turf as compared with the untreated control. 2Mean reductions include data from August 18 through September 21. Mean reductions for the EARLY POST treatments (trts 9-11 and 14) include values from July 9 through September 21. EARLY POST applications were made on July 1 and MID POST applications on July 14. NS = means are not significantly different at the 0.05 level. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Untreated control L-0051 L-0051 L-0260 L-0289 L-0259 L-0261 BAS514 50WP Dimension 1EC Preclaim 3.09 EC Preclaim 3.09 EC Preclaim 3.09 EC Preclaim 3.09 EC Acclaim Extra 0.57EW Table 5. Broadleaf weed species populations1 on September 21 in Kentucky bluegrass treated for the 1998 Postemergence Annual Weed Study. Oxalis Spurge Dandelion Clover Percentage Percentage Materials cover (%) Counts Counts Counts cover (%) 1 Untreated control 1.7 16.0 6.7 2.3 0.0 2 L-0051 7.3 3.7 13.3 5.0 0.0 3 L-0051 13.3 7.0 0.0 3.7 0.0 4 L-0260 3.3 13.3 0.3 8.3 0.0 5 L-0289 12.3 15.7 0.7 8.3 0.0 6 L-0259 4.7 1.0 7.0 3.7 0.0 7 7.0 L-0261 1.0 10.0 3.7 0.0 8 BAS514 50WP 2.7 13.7 0.3 6.7 0.0 9 Dimension 1EC 7.3 2.0 31.3 20.0 1.7 10 Preclaim 3.09 EC 1.0 0.3 32.3 18.3 8.3 11 Preclaim 3.09 EC 3.0 2.0 19.3 11.7 5.0 12 Preclaim 3.09 EC 2.3 1.3 12.3 7.0 0.3 13 Preclaim 3.09 EC 1.7 28.7 20.0 0.7 5.0 14 Acclaim Extra 0.57EW 7.3 5.7 31.3 21.7 10.0 P > F = 0.08 14.2 NS 16.0 NS NS LSD 0 0 5 ‘Count data represent the number o f Oxalis, Spurge, and Dandelion per plot. Percentage cover data represent the area per plot covered by Dandelion and Clover. Early post applications were made on July 1 when the crabgrass was in the 1-to 4-leaf stage and mid post applications on July 14 when the crabgrass had 1 to 3 tillers. NS = means are not significantly different at the 0.05 level. 35 Herbicide and Growth Regulator Studies Postemergent Broadleaf Weed Control Study Barbara R. Bingaman, Melissa C. McDade, Michael B. Faust, and Nick E. Christians The efficacy o f Drive 75DF applied alone postemergently (POST) and in combination with other postemergence broadleaf herbicides was evaluated. This study was conducted at the Iowa State University Horticulture Research Station north o f Ames, IA in 'common' Kentucky bluegrass with a dense population o f red clover, white clover, dandelions, plantain, and other assorted broadleaf weed species. The soil in this plot was a Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll) with 4.45% organic matter, 26 ppm P, 257 ppm K, and a pH o f 6.65. The experimental design was a randomized complete block. The individual plots were 5 x 10 ft with 3 replications. There were eight treatments including an untreated control. Drive 75DF was applied at 0.25, 0.50, and 0.75 lb a.i./A (Table 1). Drive 75DF also was applied at these same rates as a tank mix with 2, 4-D Amine 4.1SL at 0.75 lb a.i./A. Trimec Classic 3.32EC at 1.5 lb a.i./A and an untreated control were included for comparisons. Methylated soy soil (MSO) was added to all treatments as a carrier at 1.0% V/V. All materials were applied POST on June 5, 1998 after the broadleaf weeds were well established. Application was made at 30 psi with a carbon dioxide backpack sprayer equipped with Teejet® #8006 flat fan nozzles. The materials were diluted into 567 ml o f water that translates to an application rate o f 3 gal/1000 ft2. The study was checked for phytotoxicity and visual quality differences weekly beginning June 10 (Table 1). Visual quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. Phytotoxicity was quantified using a 9 to 1 scale with 9 = no damage, 6 = moderate damage, and 1 = dead turf. Weed control was evaluated for all broadleaf and grass species found. Dandelion and clover damage ratings were taken on June 10, 24, and 30 (Table 2). Damage was assessed using a 9 to 1 scale with 9 = no damage, 5 = plants 50% dead, 1 = dead plants. Dandelion counts were taken on July 8 and July 30 and these figures represent the number o f dandelions per plot (Table 3). Because of the large numbers o f small dandelion plants, percentage dandelion cover data were taken on July 15 and July 30 (Table 4). In plots with large numbers o f dandelions, the number in a portion o f the plot was counted and this number was then adjusted to represent the entire plot. Percentage clover cover data were taken on July 8, July 15, and July 30 (Table 5). Percentage cover data represent the area per plot covered by clover. Population assessments were made o f all weed species in each plot on July 30 (Table 6). The number o f crabgrass, spurge, plantain, purslane, and oxalis was counted per plot. All data were analyzed with the Statistical Analysis System (SAS, Version 6.12) and the Analysis o f Variance (ANOVA) procedure. Treatment effects were compared with Fisher's Least Significant Difference (LSD) test. No phytotoxic symptoms were found on any treated turf as compared with the untreated controls. None o f the materials reduced the quality o f the treated turf as compared with the untreated controls (Table 1). Some materials were causing damage to dandelion and clover as early as 5 days after treatment on June 10 (Table 2). By June 24, all treated dandelion and clover were damaged and by June 30, dead clover was found in turf treated with Drive 75DF at 0.25, 0.50, and 0.75 lb a.i./A (treatments 5-7). All materials significantly reduced dandelion counts (Table 3). Drive 75DF at 0.25, 0.50, and 0.75 lb a.i./A in tank mix with 2,4-D Amine 4.1SL and Drive 75DF at 0.75 lb a.i./A alone reduced dandelion populations > 92.5% on July 8 and > 86.2% on July 30 as compared with the untreated controls. Percentage dandelion cover was significantly lower in all treated turf as compared with the untreated controls (Table 4). On July 15 and July 30, Drive 75DF plus 2,4-D Amine 4.1SL tank mixes and Drive 75DF alone at 0.50 and 0.75 lb a.i./A reduced dandelion cover by > 90.0% as compared with the untreated controls. All treatments significantly reduced percentage clover cover as compared with the untreated controls (Table 5). Cover was reduced at least 95.9% in all treated turf for July 8, July 15, and July 30 as compared with the untreated controls. 36 Herbicide and Growth Regulator Studies The distribution o f spurge, plantain, purslane and oxalis were quite sporadic. Populations of these broadleaves were not significantly different in the treated and untreated controls (Table 6). The distribution o f crabgrass was more uniform and there was more crabgrass in all treated turf than in the untreated controls (Table 6). There were significantly more crabgrass plants in turf treated with Drive 75DF at 0.25 lb a.i./A plus 2,4-D Amine 4.1SL and Trimec Classic 3.32EC at 1.5 lb a.i./A than in the untreated controls. The small crabgrass numbers in the untreated turf can be explained by the competition from the large populations o f clover and dandelion. In the treated turf, crabgrass moved into bare areas created where dandelion and clover were killed. Table 1. Visual turf quality1 o f turf in the 1998 Postemergence Broadleaf Weed Control Study. Material2 1. 2. 3. 4. 5. 6. 7. 8. Untreated control Drive 75DF Drive 75DF Drive 75DF Drive 75DF + 2,4-D Amine 4.1SL Drive 75DF + 2,4-D Amine 4.1SL Drive 75DF + 2,4-D Amine 4.1 SL Trimec Classic 3.32EC Rate (lb a.i./A) N/A 0.25 0.50 0.75 0.25 0.75 0.50 0.75 0.75 0.75 1.50 June 10 9 9 9 9 9 June 24 7 7 7 7 7 June 30 7 7 7 7 7 July 8 7 7 7 7 7 July 15 7 7 7 7 7 July 24 7 7 7 7 7 July 30 7 7 7 7 7 9 7 7 7 7 7 7 9 7 7 7 7 7 7 9 7 7 7 7 7 7 -- - ~ LSDoos T TTT 1Visual quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable & 1 = worst quality. 2MSO was added to all treatments at 1.0% V/V. POST applications were made on June 5, 1998. —= means comparisons tests are not valid for these data. Table 2. Dandelion and clover damage1 following treatment for the 1998 Postemergence Broadleaf Weed Control _________ Study.______________________________________________________________________________________ Dandelion damage______ _______ Clover damage Material2 June June June Rate June June June 24 30 24 10 (lb a.i./A) 10 30 1. Untreated control 9.0 9.0 N/A 9.0 9.0 9.0 9.0 2. Drive 75DF 8.7 0.25 8.7 7.3 7.3 4.3 2.3 3. 5.0 Drive 75DF 0.50 9.0 8.0 4.7 8.3 2.0 4. Drive 75DF 7.0 0.75 8.7 7.0 5.0 8.0 2.0 5. Drive 75DF 0.25 9.0 7.0 8.3 7.0 1.0 3.3 + 2,4-D Amine 4.1SL 0.75 6. 2.7 4.0 Drive 75DF 0.50 8.3 6.3 7.7 1.0 + 2,4-D Amine 4.1SL 0.75 7. 7.0 Drive 75DF 2.0 8.0 0.75 8.7 7.0 1.0 + 2,4-D Amine 4.1SL 0.75 8. Trimec Classic 3.32EC 1.50 7.0 3.3 7.3 7.3 1.7 7.3 LSD 0 0 5 1.0 1.5 2.1 NS ’Damage was assessed using a 9 to 1 scale with 9 = no damage, 5 = plants 50% dead, 1 = dead plants. 2MSO was added to all treatments at 1.0% V/V. NS = means are not significantly different at the 0.05 level. 37 2.5 1.7 Herbicide and Growth Regulator Studies Table 3. Dandelion counts1 and reductions in dandelion counts2 following treatment for the 1998 Postemergence Broadleaf Weed Control Study. Material3 1. 2. 3. 4. 5. 6. 7. 8. Untreated control Drive 75DF Drive 75DF Drive 75DF Drive 75DF + 2,4-D Amine 4.1SL Drive 75DF + 2,4-D Amine 4.1SL Drive 75DF + 2,4-D Amine 4.1SL Trimec Classic 3.32EC Dandelion counts Mean July 30 July 8 210.0 95.0 65.0 29.0 8.3 343.3 109.2 69.7 32.5 7.3 0.0 74.1 84.4 92.5 98.7 0.0 54.8 69.0 86.2 96.0 90.5 97.9 3.0 3.0 3.0 99.4 98.6 99.1 1.7 1.0 1.3 99.7 99.5 99.6 107.0 71.7 89.3 77.6 65.9 74.0 79.7 67.7 56.5 16.7 32.3 16.5 Rate July (lb a.i./A) 8 N/A 0.25 0.50 0.75 0.25 0.75 0.50 0.75 0.75 0.75 1.50 476.7 123.3 74.3 36.0 6.3 LSD 0 0 5 Count reductions July 30 Mean 0 .0 6 8 .2 7 9 .7 'These figures represent the number of dandelions per plot. In plots with large populations, dandelion counts were estimated by counting the number of dandelions in a portion of the plot. Smaller counts in the untreated controls were recorded on July 30 than on July 15. This could be explained by the large increase in the percentage clover cover and the difficulty in finding dandelions in a dense clover cover. deductions in dandelion counts are expressed as percentages of the untreated controls. 3MSO was added to all treatments at 1.0% V/V. POST applications were made on June 5, 1998. Table 4. Percentage dandelion cover1 and reductions in cover2 following treatment for the BASF Postemergence _________ Broadleaf Study.____________________________________________________________________________ Rate Material3 (lba.i./A) Percentage dandelion cover July July 15 30 Mean ________ Cover reductions_____ July July 15 30 Mean % 1. 2. 3. 4. 5. 6. 7. 8. Untreated control Drive 75DF Drive 75DF Drive 75DF Drive 75DF + 2,4-D Amine 4.1SL Drive 75DF + 2,4-D Amine 4.1SL Drive 75DF + 2,4-D Amine 4.1SL Trimec Classic 3.32EC LSD 0 0 5 N/A 0.25 0.50 0.75 0.25 0.75 0.50 0.75 0.75 0.75 1.50 23.3 6.7 2.3 0.7 0.3 25.0 6.7 2.3 2.3 1.0 24.2 6.7 2.3 1.5 0.7 71.4 90.0 97.1 98.6 0.0 73.3 90.7 90.7 96.0 0.0 72.5 90.4 93.8 97.2 0.3 0.7 0.5 98.6 97.3 97.9 0.0 0.7 0.3 1 0 0 .0 97.3 98.6 5.3 4.0 4.7 77.1 84.0 80.7 4.0 4.5 4.0 17.1 17.9 16.4 0.0 'Percentage dandelion cover was determined by estimating the area per plot covered by dandelions. These estimations were made to more accurately depict the dandelion populations in treated plots, deductions in dandelion cover are expressed as percentages of the untreated controls. 3MSO was added to all treatments at 1.0% V/V. POST applications were made on June 5, 1998. 38 Herbicide and Growth Regulator Studies Table 5. Percentage clover cover 1 and reductions in clover cover2 following treatment for the BASF Postemergence _________ Broadleaf Study._______________________________________________________________________________ Material3 Rate (lba.i./A) Untreated control Drive 75DF Drive 75DF Drive 75DF Drive 75DF + 2,4-D Amine 4.1SL Drive 75DF + 2,4-D Amine 4.1SL Drive 75DF + 2,4-D Amine 4.1SL Trimec Classic 3.32EC N/A 0.25 0.50 0.75 0.25 0.75 0.50 0.75 0.75 0.75 1.50 Percentage clover cover July July July 8 15 30 Mean ________ Cover reductions_______ July July July 8 15 30 Mean % 1. 2. 3. 4. 5. 6. 7. 8. LSD 0.05 56.7 2.3 0.0 0.7 1.3 56.7 2.0 0.7 0.0 0.3 73.3 2.3 0.7 0.3 2.3 62.2 2.2 0.4 0.3 1.3 . 0.0 95.9 100.0 98.8 97.6 0.0 96.5 98.8 100.0 99.4 0.0 96.8 99.1 99.5 96.8 0.0 96.4 99.3 99.5 97.9 0.3 0.0 0.3 0.2 99.4 100.0 99.5 99.6 0.0 0.0 0.0 0.0 100.0 100.0 100.0 100.0 1.7 2.0 0.7 1.4 97.1 96.5 99.1 97.7 6.9 12.5 7.1 8.0 12.2 22.1 9.7 12.9 1Percentage clover cover was determined by estimating the area per plot covered by clover, deductions in clover cover are expressed as percentages of the untreated controls. 3MSO was added to all treatments at 1.0% V/V. POST applications were made on June 5, 1998. Table 6. Crabgrass, spurge, plantain, purslane, and oxalis counts1 following treatment for the BASF Postemergence _________ Broadleaf Study._______________________________________________________________________________ Material2 Rate Crabgrass Spurge Plantain 2.0 0 .0 4.3 4.7 3.3 13.7 1 .3 0 .7 0 .3 0 .0 0 .3 2 .3 1.7 0.3 0.3 0.3 0.0 0 .0 0 .3 7.3 2 .3 0.0 0 .3 0 .3 4.7 2 .3 0.0 1 .3 0 .3 12.3 0 .7 0.0 0 .3 0 .0 7.4 NS NS NS NS Purslane Oxalis (lb a.i./A) 1. 2. 3. 4. 5. 6. 7. 8. Untreated control Drive 75DF Drive 75DF Drive 75DF Drive 75DF + 2,4-D Amine 4.1SL Drive 75DF + 2,4-D Amine 4.1SL Drive 75DF + 2,4-D Amine 4.1SL Trimec Classic 3.32EC N/A 0.25 0.50 0.75 0.25 0.75 0.50 0.75 0.75 0.75 1.50 LSD 0.05 1T M figures represent the number of plants per plot. These 3 .7 2 .7 2MSO was added to all treatments at 1.0% V/V. NS = means are not significantly different at the 0.05 level. 39 0 .0 0 .0 0 .0 0 .0 Herbicide and Growth Regulator Studies Postemergent Granular & Sprayable Broadleaf Weed Control Study Barbara R. Bingaman, Melissa C. McDade, Michael B. Faust, and Nick E. Christians A field study was initiated to compare the efficacy of several new granular broadleaf weed and feed products to the standard herbicides in applications made to both damp and dry foliage. Sprayable formulations also were screened to: 1) determine the efficacy o f Millenium Ultra, Horsepower, DTD A, XRM-5202, and other research numbered compounds to traditional 3-way herbicides; 2) determine the efficacy differential between the optical and racemic isomers o f 2, 4-DP and MCPP; 3) determine the efficacy of Confront as compared to traditional 3-way herbicides. This study was conducted at the Iowa State University Horticulture Research Station north o f Ames, IA in an area of common bluegrass with a heavy infestation of red clover, white clover, dandelions, and assorted broadleaf weed species. The soil in this area was a Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll) with an organic matter content of 4.45%, a pH o f 6.65, 26 ppm P, and 257 ppm K. The experimental plots were 5 x 10 ft with three replications and two rows per replication. The study was comprised o f 34 treatments including eight granular materials applied to both wet and dry foliage (Table 1). Those plots receiving the granular materials were split into two 2 1/2 x 10 ft plots. The northern one was wet with water just prior to application and the southern was kept dry. Half o f the total material for these plots was applied to the wet foliage and half to the dry. The sprayable materials included Millenium Ultra, Horsepower, DTD A, XRM 5202, Confront, RDL202, RDL211, and RDL414. Super Trimec and Trimec Classic were included as industry standards and Triplet as a 3-way herbicide standard. The racemic (R) and optical (O) formulations o f Dissolve, MCPP, and 2,4-DP also were screened. An untreated control was included. Granular materials were applied using cardboard containers as 'shaker dispensers'. Sprayables were applied at 30-35 psi using a carbon dioxide backpack sprayer equipped with TeeJet® #8006 flat fan nozzles. The liquids were diluted into 567 ml o f water. This translates to an application rate o f 3 gal/1000 ft2. All products were applied postemergently after dandelions, clover, and other broadleaf species were well established. Applications were made on June 5, 1998. The plot was neither irrigated nor mowed for 24 hours following application. Turf quality and phytotoxicity data were taken from June 10 through July 30 (Table 2). Visual quality was assessed using a scale from 9 to 1 with 9 = best, 6 = lowest acceptable, and 1 = worst quality. Phytotoxicity was recorded as present or absent. Broadleaf and grass weed species were monitored. Weed damage data were taken on June 10 (Table 3). Damage to dandelions and clover was assessed using a 9 to 1 scale with 9 = no damage, 5 = 50% o f plants showing damage, and 1 = 100% o f plants exhibiting damage. The symptoms o f damage included foliage discoloration, wilting, and curling. Percentage dandelion and clover mortality data were taken on June 24 and June 30 (Table 4). Mortality was assessed by estimating the number o f living plants per plot and comparing to the untreated controls. Dandelion counts (Table 5) and percentage clover cover (Table 7) data were collected on July 7, July 14, July 24, and July 30 . For these data, the number o f dandelions was counted and the percentage clover cover was estimated for each plot. Reductions were calculated as percentages o f the untreated controls on each collection date for dandelion (Table 6) and clover (Table 8). Percentage crabgrass cover data were taken on July 7, July 14, July 24, and July 30 (Table 9). Reductions were not calculated since there was more crabgrass in many of the treated plots than in the untreated controls. The only other weed species with a widespread distribution was spurge. The number o f spurge per plot was counted (Table 10). Reductions in numbers were not calculated because there were no spurge plants in the untreated controls. All data were analyzed with the Statistical Analysis System (SAS, Version 6.12) and the Analysis of Variance (ANOVA) procedure. Fisher's Least Significant Difference (LSD) test was utilized for means comparisons. 40 Herbicide and Growth Regulator Studies No phytotoxicity was found during this study. Visual quality of turf treated with the herbicide plus fertilizer formulations was better than the untreated and unfertilized turf (Table 2). The numerical differences were not statistically significant. The turf treated with the herbicide plus fertilizer materials had consistently improved quality over the entire duration o f the study as compared with the untreated and unfertilized turf. Many o f the sprayable materials were causing significant levels of damage to dandelion and clover as compared with the untreated control on June 10 (Table 3). Dandelion and clover treated with the granular formulations were not exhibiting significant damage on this date. By June 30, six o f the sprayable materials had caused dandelion mortality > 90% as compared with the untreated control (Table 4). On this date, 11 of the sprayable formulations and two granulars (applied to wet foliage) had killed all o f the clover. Sixteen o f the sprayable materials significantly decreased dandelion counts on July 7 and all sprayables significantly suppressed dandelions July 14 through July 30 as compared to the untreated controls (Table 5). Treatment with the granular formulations applied to either wet or dry foliage resulted in significantly fewer dandelions from July 7 through July 30 as compared to the untreated controls. On July 7, 12 sprayable herbicides reduced dandelion populations > 94% as compared with the untreated control (Table 6). By July 30, only eight sprayables were providing this level of reduction. The mean level of control was > 91.4% for 10 sprayable materials as compared with the untreated control. One granular formulation reduced dandelion populations > 90% and two granulars produced reductions > 83.4% as compared with the untreated controls on July 7 (Table 6). Three granulars provided dandelion control that was > 80.5% on July 30. For some o f the granular materials, significantly better control was achieved when the material was applied to wet foliage than to dry (Table 11). Mean dandelion control was significantly better for five of the granular materials when applied to wet foliage than to dry. All sprayable and granular herbicides significantly suppressed clover cover July 7 through July 30 as compared with the untreated controls (Table 7). Twenty-three materials kept clover cover below 5.0% on July 7 as compared to the untreated control. The mean clover cover was < 5.0% for 20 of the materials and 12 of these kept clover cover <1.0% for the entire duration o f the study. Twenty-three materials reduced clover cover > 92% on July 7 as compared with the untreated control and 11 o f these killed all clover (Table 8). Mean clover cover reductions were > 94% for 20 of the herbicide formulations. Clover control was similar for most granular materials applied on wet and on dry foliage (Table 12). There were numerical differences in reduction of clover cover between the dry and wet applications but they were not statistically significant. Percentage crabgrass cover was significantly higher in turf treated with nine of the sprayable materials than in the untreated controls on July 14 (Table 9). By July 30, crabgrass cover was > 20% in turf treated with 13 o f the herbicides and was < 1% in the untreated control. The crabgrass populations in the treated turf can be explained by the reduced competition from dandelion and clover as compared with competition in the untreated turf. Spurge numbers were significantly higher in some treated turf as compared with the untreated control (Table 10). These populations also can be explained by the reduced competition in the treated turf as compared with the untreated turf. 41 Herbicide and Growth Regulator Studies Table 1. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 'The 5 x Herbicides applied on June 5 for the 1998 Postemergence Granular and Sprayable Broadleaf Weed Control Study. Formulation Rate oz/1000 ft2 Material Untreated control Millenium Ultra 47.10% Horsepower 50.40% DTDA 47.30% XRM 5202 55.80% Confront 45.10% Triplet (std) 49.67% RDL202 89.76%DF RDL211 55.98% RDL414 55.48% MCPP (R) 51.90% MCPP (O) 25.96% 2,4-DP (R) 50.00% 2,4-DP (O) 25.00% Dissolve (R) 57.70% Dissolve (O) 43.31% Super Trimec Trimec Classic 3.4SL N/A 0.92 0.92 0.92 1.29 0.74 1.20 0.61 1.10 1.10 1.10 1.10 1.10 1.10 1.50 1.50 1.10 1.50 N/A sprayable sprayable sprayable sprayable sprayable sprayable sprayable sprayable sprayable sprayable sprayable sprayable sprayable sprayable sprayable sprayable sprayable Dry or wet foliage N/A diy dry dry dry diy dry dry dry dry dry dry dry dry dry dry dry dry Rate lb/1000 ft2 granular wet Millenium U ltra W & F -1 8 ' 3.60 granular wet Trupower W & F - 18l 3.60 granular wet 3.60 Dissolve W & F - 181 wet granular 3.60 Dissolve Ultra W & F - 18' 3.60 granular wet Horsepower W & F - 181 granular wet XRM 5202 W & F - 1 8 1 3.60 granular wet 2.94 Turf Builder + 21 wet granular 3.60 Fertilized control1 dry granular 3.60 Millenium Ultra W & F - 1 8 1 dry 3.60 granular Trupower W & F - 181 dry granular 3.60 Dissolve W & F - 18' dry granular 3.60 Dissolve Ultra W & F - 18* dry 3.60 granular Horsepower W & F - 181 dry granular 3.60 XRM 5202 W & F - 1 8 1 dry granular 2.94 Turf Builder + 21 dry granular 3.60 Fertilized control1 10 ft plots for these treatments were split into two 2 1/2 x 10 ft subplots. One-half of each material was applied to a dry and a wet subplot. Table 2. Visual quality1of turf treated on June 5 for the 1998 Postemergence Granular and Sprayable Broadleaf Weed Control Study. July 7 July 14 July 24 July 30 June 10 June 30 June 24 Material 6 6 6 6 7 7 7 1 Untreated control 6 7 7 6 6 6 7 Millenium Ultra 47.10% 2 6 6 7 7 6 6 7 3 Horsepower 50.40% 6 6 6 6 7 7 7 4 DTDA 47.30% 6 6 6 7 7 6 7 5 XRM 5202 55.80% 6 7 6 6 6 7 7 6 Confront 45.10% 6 6 6 7 7 6 7 7 Triplet (std) 49.67% 6 7 6 6 6 7 7 8 RDL202 89.76%DF 6 6 7 7 6 6 7 9 RDL211 55.98% 6 7 6 6 7 7 6 10 RDL414 55.48% 6 6 6 7 7 6 MCPP (R) 51.90% 7 11 6 6 6 6 7 7 7 MCPP (O) 25.96% 12 6 6 6 7 7 6 7 2,4-DP (R) 50.00% 13 6 6 6 6 7 7 7 14 2,4-DP (O) 25.00% 6 6 6 6 7 7 7 15 Dissolve (R) 57.70% 6 6 7 7 6 6 7 16 Dissolve (O) 43.31% 6 6 7 6 6 7 7 17 Super Trimec 6 6 6 6 7 7 7 18 Trimec Classic 3.4SL 7 7 • 7 7 8 8 8 Millenium Ultra W & F -182 19 7 7 7 7 8 Trupower W & F - 182 8 8 20 7 7 7 7 8 8 8 Dissolve W & F - 182 21 7 7 7 7 8 8 8 Dissolve Ultra W & F - 1 8 2 22 7 7 7 7 8 8 8 Horsepower W & F - 182 23 7 7 7 7 8 8 8 XRM 5202 W & F - 182 24 7 7 7 8 8 7 8 Turf Builder+ 22 25 7 7 7 7 8 8 8 26 Fertilized control2 7 7 7 7 8 8 8 Millenium Ultra W & F -182 27 7 7 7 7 8 8 8 Trupower W & F - 182 28 7 7 7 7 8 8 8 Dissolve W & F - 1 8 2 29 7 7 7 7 8 8 8 Dissolve Ultra W & F - 1 8 2 30 7 7 7 7 8 8 8 Horsepower W & F - 182 31 7 7 7 7 8 8 8 XRM 5202 W & F - 1 8 2 32 7 7 7 7 8 8 8 Turf Builder + 22 33 7 7 7 7 8 8 8 34 Fertilized control2 'U r .‘Visual1 quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. 2The 5 x 10 ft plots for these granular treatments were split into two 2 1/2 x 10 ft subplots. One-half of each material was applied to a dry and a wet subplot. LSD's were not included because means comparisons were not appropriate for these data. 42 Herbicide and Growth Regulator Studies Table 3. Dandelion and clover damage1 on June 10 in turf treated on June 5 for the 1998 Postemergence Granular and Sprayable Broadleaf Weed Control Study. Material__________________________________________________________________ Dandelion______________________________ Clover 9.0 1 9.0 Untreated control 7.0 2 Millenium Ultra 47.10% 5.7 6.7 5.7 3 Horsepower 50.40% 5.7 4 DTDA 47.30% 5.7 7.0 5 7.0 XRM 5202 55.80% 7.7 6 5.0 Confront 45.10% 6.3 7 Triplet (std) 49.67% 6.0 6.0 8 5.3 RDL202 89.76%DF 5.7 9 5.7 RDL211 55.98% 4.3 10 RDL414 55.48% 5.3 8.7 8.0 11 MCPP (R) 51.90% 7.0 7.3 12 MCPP (0)25.96% 8.7 13 8.3 2,4-DP (R) 50.00% 7.3 14 2,4-DP (O) 25.00% 8.0 7.3 15 7.0 Dissolve (R) 57.70% 7.0 16 6.7 Dissolve (0)43.31% 6.3 5.0 17 Super Trimec 8.0 18 Trimec Classic 3.4SL 7.0 8.3 19 8.7 Millenium Ultra W & F -182 8.0 20 Trupower W & F - 182 8.7 8.3 21 Dissolve W & F - 182 8.3 7.7 22 Dissolve Ultra W & F - 1 8 2 8.0 8.7 23 Horsepower W & F - 182 8.7 24 8.0 XRM 5202 W & F - 182 8.3 25 8.3 8.0 Turf Builder + 22 26 9.0 Fertilized control2 9.0 27 Millenium Ultra W & F -182 8.3 8.3 28 8.0 Trupower W & F - 182 8.7 29 8.3 Dissolve W & F - 182 8.3 30 Dissolve Ultra W & F - 1 8 2 7.7 8.0 31 Horsepower W & F - 182 8.7 8.7 32 8.0 XRM 5202 W & F - 182 8.3 33 8.3 Turf Builder + 22 8.0 34 Fertilized control2 9.0 9.0 1.8 1.7 LSD 005 'Damage was assessed using a 9 to 1 scale with 9 = no damage, 5 = 50% of plants showing damage, and 1 = 100% of plants exhibiting damage. Symptoms of damage included foliage discoloration, wilting, and curling. The 5 x 10 ft plots for these granular treatments were split into two 2 1/2 x 10 ft subplots. One-half of each material was applied to a dry and a wet subplot. Table 4. Percentage dandelion and clover cover1reductions in turf treated on June 5 for the 1998 Postemergence Granular and Sprayable Broadleaf Weed Control Study. Clover cover Dandelion cover June 30 Mean June 24 Mean Material June 24 June 30 % 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Untreated control Millenium Ultra 47.10% Horsepower 50.40% DTDA 47.30% XRM 5202 55.80% Confront 45.10% Triplet (std) 49.67% RDL202 89.76%DF RDL211 55.98% RDL414 55.48% MCPP (R) 51.90% MCPP (O) 25.96% 2,4-DP (R) 50.00% 2,4-DP (O) 25.00% Dissolve (R) 57.70% Dissolve (O) 43.31% Super Trimec Trimec Classic 3.4SL Millenium Ultra W & F -182 Trupower W & F - 182 Dissolve W & F - 1 8 2 Dissolve Ultra W & F - 1 8 2 Horsepower W & F - 182 XRM 5202 W & F - 1 8 2 Turf Builder + 22 Fertilized control2 Millenium Ultra W & F - 1 8 2 Trupower W & F - 182 Dissolve W & F - 1 8 2 Dissolve Ultra W & F - 1 8 2 Horsepower W & F - 182 XRM 5202 W & F - 1 8 2 Turf Builder + 22 Fertilized control2 0.0 0.0 0.0 0.0 0.0 0.0 56.7 70.0 53.3 36.7 73.3 46.7 40.0 53.3 73.3 46.7 26.7 40.0 56.7 63.3 73.3 73.3 70.0 43.3 43.3 30.0 33.3 33.3 36.7 53.3 86.7 73.3 86.7 83.3 100.0 80.0 100.0 100.0 90.0 36.7 43.3 30.0 36.7 80.0 76.7 100.0 93.3 56.7 63.3 20.0 23.3 10.0 60.0 43.3 71.7 71.7 70.0 60.0 86.7 63.3 70.0 76.7 81.7 41.7 35.0 35.0 46.7 71.7 75.0 86.7 81.7 50.0 53.3 25.0 28.3 21.7 48.3 48.3 63.3 46.7 33.3 36.7 66.7 43.3 40.0 46.7 70.0 23.3 20.0 30.0 26.7 43.3 53.3 63.3 76.7 36.7 60.0 20.0 33.3 33.3 40.0 26.7 100.0 100.0 60.0 100.0 100.0 100.0 100.0 100.0 100.0 50.0 10.0 56.7 40.0 83.3 100.0 100.0 100.0 100.0 100.0 33.3 81.7 73.3 46.7 68.3 83.3 71.7 70.0 73.3 85.0 36.7 15.0 43.3 33.3 63.3 76.7 81.7 88.3 68.3 80.0 26.7 16.7 16.7 53.3 21.7 0.0 0.0 0.0 30.0 30.0 6.7 23.3 33.3 36.7 40.0 46.7 33.3 26.7 30.0 38.3 31.7 3.3 20.0 16.7 31.7 35.0 0.0 0.0 0.0 0.0 16.7 0.0 • 0.0 0.0 66.7 16.7 0.0 0.0 0.0 30.0 53.3 13.3 23.3 30.0 40.0 26.7 76.7 76.7 33.3 20.0 16.7 53.3 65.0 23.3 11.7 15.0 30.0 21.7 0.0 0.0 0.0 0.0 0.0 6.8 33.4 41.5 25.6 28.0 28.7 ‘These figures represent percentage reductions in percentage dandelion and clover cover per plot as compared with the untreated controls. The 5 x 10 ft plots for these granular treatments were split into two 2 1/2 x 10 ft subplots. One-half of each material was applied to a dry and a wet subplot. LSDoos 43 Herbicide and Growth Regulator Studies Table 5. Dandelion counts1 in turf treated for the 1998 Postemergence Granular and Spray able Broadleaf Weed Control Study. July 7 July 24 July 30 Mean count Material July 14 342.8 316.7 366.7 390.0 1 Untreated control 298.0 1.7 1.9 1.3 Millenium Ultra 47.10% 2.3 2 2.3 77.7 9.7 35.0 66.7 47.3 3 Horsepower 50.40% 29.6 36.7 41.7 4 DTDA 47.30% 16.7 23.3 26.7 25.7 17.3 5 XRM 5202 55.80% 2.0 15.0 5.7 5.7 3.5 6 Confront 45.10% 0.3 2.3 44.7 37.6 10.7 21.7 73.3 7 Triplet (std) 49.67% 15.0 10.6 RDL202 89.76%DF 10.0 15.0 8 2.3 18.3 12.3 4.0 11.7 15.0 9 RDL211 55.98% 13.7 9.7 13.3 8.3 10 RDL414 55.48% 3.3 151.7 132.6 141.7 112.0 125.0 MCPP (R) 51.90% 11 183.3 191.3 183.3 185.0 173.3 MCPP (O) 25.96% 12 216.7 209.7 216.7 200.0 205.3 13 2,4-DP (R) 50.00% 210.0 200.0 212.3 216.7 222.7 14 2,4-DP (O) 25.00% 20.3 14.8 18.3 7.0 13.3 15 Dissolve (R) 57.70% 125.0 123.0 104.5 80.0 90.0 Dissolve (0)43.31% 16 13.0 10.1 9.0 8.7 9.7 17 Super Trimec 20.0 16.7 13.3 4.3 12.0 18 Trimec Classic 3.4SL 44.7 34.7 46.7 10.7 36.7 19 Millenium Ultra W & F - 1 8 2 106.7 111.3 82.7 63.3 20 Trupower W & F - 182 49.3 186.7 180.0 166.7 200.0 166.7 Dissolve W & F - 1 8 2 21 100.0 110.0 103.3 Dissolve Ultra W & F - 1 8 2 103.3 100.0 22 73.3 76.0 73.2 Horsepower W & F - 182 70.0 73.3 23 91.3 72.7 66.7 63.3 69.3 XRM 5202 W & F - 182 24 58.7 90.0 41.3 40.0 63.3 25 Turf Builder + 22 360.0 366.7 314.7 266.7 265.3 26 Fertilized control2 76.7 143.3 133.3 111.7 Millenium Ultra W & F - 1 8 2 93.3 27 253.3 164.7 250.0 217.0 200.0 28 Trupower W & F - 182 286.7 244.1 Dissolve W & F - 1 8 2 179.7 226.7 283.3 29 230.0 225.5 223.3 233.3 30 Dissolve Ultra W & F - 1 8 2 215.3 260.0 290.0 249.2 216.7 230.0 Horsepower W & F - 182 31 226.7 216.7 190.3 XRM 5202 W & F - 182 138.0 180.0 32 108.7 115.0 104.7 133.3 113.3 33 Turf Builder + 22 366.7 324.5 281.3 316.7 333.3 34 Fertilized control2 76.4 77.2 70.7 78.8 85.7 LSDoos 'These figures represent the number of dandelions per plot. The 5 x 10 ft plots for these granular treatments were split into two 2 1/2 x 10 ft subplots. One-half of each material was applied to a dry and a wet subplot. Table 6. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Percentage reductions in dandelion counts' in treated turf as compared with the untreated controls of the 1998 Postemergence Granular and Sprayable Broadleaf Weed Control Study Mean reduction July 24 July 30 July 7 July 14 Material n/ % 0.0 0.0 0.0 0.0 0.0 Untreated control 99.7 99.4 99.5 Millenium Ultra 47.10% 99.2 99.3 81.8 80.1 86.2 88.9 96.8 Horsepower 50.40% 91.4 89.3 90.0 94.4 92.6 DTDA 47.30% 94.9 93.4 92.7 99.3 95.3 XRM 5202 55.80% 98.5 99.0 98.5 99.9 99.3 Confront 45.10% 88.5 89.0 80.0 96.4 93.2 Triplet (std) 49.67% 96.2 96.9 95.9 99.2 96.8 RDL202 89.76%DF 96.4 95.3 95.9 98.7 96.3 RDL211 55.98% 96.5 97.2 96.4 97.4 98.9 RDL414 55.48% 61.1 61.4 61.3 60.5 62.4 MCPP (R) 51.90% 50.9 46.5 50.0 37.9 45.3 MCPP (O) 25.96% 44.4 38.8 31.6 45.5 31.1 2,4-DP (R) 50.00% 46.2 38.1 45.5 31.6 25.3 2,4-DP (O) 25.00% 94.8 95.7 95.0 97.7 95.8 Dissolve (R) 57.70% 68.5 69.5 65.9 71.6 73.2 Dissolve (0)43.31% 96.7 97.1 97.4 97.0 97.3 Super Trimec 94.9 96.1 95.5 98.5 96.2 Trimec Classic 3.4SL 88.5 89.9 87.3 96.4 88.4 Millenium Ultra W & F - 1 8 2 71.5 75.9 70.9 80.0 83.4 Trupower W & F - 182 52.1 47.5 54.5 36.8 44.1 Dissolve W & F - 1 8 2 72.7 71.8 69.9 68.4 65.3 Dissolve Ultra W & F - 182 80.5 78.7 80.0 76.8 76.5 Horsepower W & F - 182 76.6 78.8 82.7 78.9 76.7 XRM 5202 W & F - 1 8 2 89.4 82.9 80.0 75.5 86.6 Turf Builder + 22 7.7 8.2 0.0 15.8 11.0 Fertilized control2 65.8 67.4 60.9 70.5 74.3 Millenium Ultra W & F -182 35.0 36.7 31.8 36.8 44.7 Trupower W & F - 182 28.8 26.5 22.7 39.7 28.4 Dissolve W & F - 182 41.0 36.4 34.2 27.7 29.5 Dissolve Ultra W & F - 182 33.3 27.3 20.9 27.4 27.3 Horsepower W & F - 182 41.9 44.5 40.9 53.7 43.2 XRM 5202 W & F - 1 8 2 72.1 66.5 69.1 64.9 57.9 Turf Builder + 22 6.0 5.3 9.1 0.0 5.6 Fertilized control2 19.8 20.6 20.8 26.5 27.1 LSDoos 'These figures represent percentage reductions in dandelion numbers per plot as compared with the untreated controls. 2The 5 x 10 ft plots for these granular treatments were split into two 2 1/2 x 10 ft subplots. One-half of each material was applied to a dry and a wet subplot. 44 Herbicide and Growth Regulator Studies Table 7. Percentage clover cover1in turf treated for the 1998 Postemergence Granular and Sprayable Broadleaf Weed Control Study. July 30 July 24 Material July 7 July 14 ! Untreated control 50.0 Millenium Ultra 47.10% 0.3 Horsepower 50.40% 0.0 DTDA 47.30% 1.7 XRM 5202 55.80% 0.0 Confront 45.10% 0.0 Triplet (std) 49.67% 0.0 RDL202 89.76%DF 0.0 0.0 RDL211 55.98% RDL414 55.48% 0.0 MCPP (R) 51.90% 5.3 MCPP (O) 25.96% 13.3 2,4-DP (R) 50.00% 3.7 2,4-DP (O) 25.00% 17.3 Dissolve (R) 57.70% 0.7 0.3 Dissolve (O) 43.31% Super Trimec 0.0 Trimec Classic 3.4SL 0.0 0.0 Millenium Ultra W & F -1 8 2 Trupower W & F - 182 0.7 Dissolve W & F - 182 18.7 Dissolve Ultra W & F - 182 8.3 Horsepower W & F - 182 2.3 2.0 XRM 5202 W & F - 182 4.0 Turf Builder + 22 26.7 Fertilized control2 0.0 Millenium Ultra W & F -1 82 0.7 Trupower W & F - 182 Dissolve W & F - 182 27.0 Dissolve Ultra W & F - 182 13.3 4.3 Horsepower W & F - 182 1.7 XRM 5202 W & F - 182 10.0 Turf Builder + 22 26.7 Fertilized control2 16.3 LSD o.05 'These figures the area per plot covered by clover. The 5 x 10 ft plots for these granular treatments were split into two 2 1/2 x 10 ft subplots. 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Table 8. 61.7 0.0 0.0 0.7 0.0 0.3 0.3 0.0 0.3 0.3 12.0 18.7 8.7 25.3 0.7 0.7 0.0 0.3 0.0 0.7 12.0 13.3 2.3 2.3 5.7 23.3 0.0 0.3 28.7 13.3 7.0 1.0 11.7 20.3 19.7 % 65.0 0.0 0.0 3.3 0.7 0.3 2.0 8.3 0.3 0.0 18.3 28.7 15.3 27.0 0.7 1.0 0.3 0.3 6.7 0.7 13.7 20.0 4.3' 5.3 13.3 38.3 0.0 0.3 33.7 26.7 14.0 3.7 21.7 53.3 21.2 78.3 0.0 0.0 3.3 0.7 0.3 2.0 0.0 0.3 0.0 21.7 33.7 13.7 31.7 2.0 1.0 0.3 0.7 0.0 0.7 17.0 20.0 4.3 5.3 15.0 41.7 0.0 0.3 35.3 35.0 15.7 3.7 28.3 56.7 24.1 Mean cover 63.8 0.1 0.0 2.3 0.3 0.3 1.1 2.1 0.3 0.1 14.3 23.6 10.3 25.3 1.0 0.8 0.2 0.3 1.7 0.7 15.3 15.4 3.3 3.8 9.5 32.5 0.0 0.4 31.2 22.1 10.3 2.5 17.9 39.3 19.1 One-half of each material was applied to a dry and a wet subplot. Percentage clover cover reductions1in treated turf as compared with the untreated controls of the 1998 Postemergence Granular and Sprayable Broadleaf Weed Control Study. July 30 Mean July 7 July 14 July 24 Material /o 0.0 0.0 0.0 0.0 0.0 Untreated control 100.0 100.0 99.9 100.0 Millenium Ultra 47.10% 99.3 100.0 100.0 100.0 100.0 100.0 3 Horsepower 50.40% 95.7 96.5 98.9 94.9 4 DTDA 47.30% 96.7 99.1 99.5 99.0 100.0 100.0 5 XRM 5202 55.80% 99.6 99.5 99.6 6 100.0 99.5 Confront 45.10% 96.9 97.4 98.3 7 100.0 99.5 Triplet (std) 49.67% 100.0 96.7 8 100.0 87.2 100.0 RDL202 89.76%DF 99.6 99.6 9 99.5 100.0 RDL211 55.98% 99.5 100.0 100.0 99.9 10 100.0 99.5 RDL414 55.48% 71.8 72.3 80.6 77.5 MCPP (R) 51.90% 11 89.3 57.0 63.0 12 69.7 55.9 MCPP (O) 25.96% 73.3 82.5 83.8 86.0 76.4 13 2,4-DP (R) 50.00% 92.7 14 58.9 58.5 59.6 60.3 2,4-DP (O) 25.00% 65.3 99.0 97.4 98.4 15 98.7 98.9 Dissolve (R) 57.70% 98.5 98.7 98.8 16 98.9 Dissolve (O) 43.31% 99.3 99.6 99.7 17 100.0 100.0 99.5 Super Trimec 99.5 99.1 18 100.0 99.5 99.5 Trimec Classic 3.4SL 100.0 100.0 89.7 97.4 19 Millenium Ultra W & F -182 100.0 99.0 99.1 99.4 20 Trupower W & F - 182 98.7 98.9 79.0 78.3 75.9 21 Dissolve W & F - 1 8 2 62.7 80.6 74.5 75.8 78.4 69.2 22 Dissolve Ultra W & F - 182 83.3 94.5 93.3 94.8 23 95.3 96.2 Horsepower W & F - 182 93.2 91.8 94.1 24 XRM 5202 W & F - 182 96.0 96.2 80.8 85.1 79.5 25 92.0 90.8 Turf Builder + 22 46.8 49.0 41.0 26 46.7 62.2 Fertilized control2 100.0 100.0 100.0 27 100.0 Millenium Ultra W & F -182 100.0 99.6 99.5 99.3 28 98.7 99.5 Trupower W & F - 182 54.9 48.2 51.1 29 53.5 Dissolve W & F - 182 46.0 30 78.4 59.0 55.3 65.4 Dissolve Ultra W & F - 182 73.3 80.0 83.9 31 Horsepower W & F - 182 91.3 88.7 78.5 94.4 95.3 98.4 96.1 96.7 32 XRM 5202 W & F - 182 66.7 63.8 71.9 33 Turf Builder + 22 80.0 81.1 18.0 27.6 34 67.0 38.4 Fertilized control2 46.7 30.7 32.6 29.9 31.9— —— — — —— LSD0.05 '■ ' — — — — —— .......... I I ■ — II I I. I — — I I 32.5 — —— — —— — These figures represent percentage reductions in clover cover per plot as compared with the untreated controls. 2The 5 x 10 ft plots for these granular treatments were split into two 2 1/2 x 10 ft subplots. One-half of each material was applied to a dry and a wet subplot. 1 r 45 Herbicide and Growth Regulator Studies Table 9. Percentage crabgrass cover1 in treated turf for the 1998 Postemergence Granular and Sprayable Broadleaf Weed Control Study. July 30 Material July 7 July 24 July 14 Mean • % 5.7 30.0 26.7 21.7 16.7 15.0 15.0 16.7 25.0 23.3 15.0 18.3 11.7 18.3 7.3 31.7 23.3 10.0 23.3 28.3 28.3 28.3 6.7 13.3 13.3 10.0 15.3 23.3 5.0 16.7 6.7 8.3 8.3 4.0 NS 1 0.7 1.8 0.7 Untreated control 0.3 38.3 29.3 Millenium Ultra 47.10% 18.7 30.0 30.0 18.8 Horsepower 50.40% 5.3 13.3 25.0 0.7 15.2 DTDA 47.30% 13.3 11.7 28.3 15.1 XRM 5202 55.80% 3.7 12.0 9.0 Confront 45.10% 3.7 5.3 0.7 15.0 9.1 5.7 Triplet (std) 49.67% 18.3 12.7 RDL202 89.76%DF 5.3 10.3 30.0 21.4 RDL211 55.98% 10.7 20.0 23.3 17.6 10.3 13.3 RDL414 55.48% 11.7 8.1 MCPP(R) 51.90% 0.3 5.3 18.3 10.7 1.0 5.0 MCPP (0)25.96% 8.7 6.6 0.7 5.3 2,4-DP (R) 50.00% 20.0 1.7 8.3 12.1 2,4-DP (O) 25.00% 12.7 7.3 Dissolve (R) 57.70% 2.0 7.0 31.7 21.8 10.3 13.3 Dissolve (O) 43.31% 4.0 26.7 16.7 17.7 Super Trimec 3.7 Trimec Classic 3.4SL 5.3 2.3 5.3 5.0 7.0 Millenium Ultra W & F -182 28.3 15.9 3.7 27.0 17.0 Trupower W & F - 182 9.0 6.7 18.7 Dissolve W & F - 182 10.0 15.9 Dissolve Ultra W & F - 1 8 2 7.0 30.0 11.7 19.3 Horsepower W & F - 182 1.0 5.7 0.3 3.4 XRM 5202 W & F - 1 8 2 0.7 8.7 21.7 11.1 6.7 Turf Builder + 22 2.0 2.0 6.0 Fertilized control2 0.0 10.3 3.7 6.0 Millenium Ultra W & F - 1 8 2 0.3 4.0 15.3 8.8 Trupower W & F - 182 3.7 5.3 13.3 11.4 3.7 Dissolve W & F - 1 8 2 6.7 6.7 5.5 Dissolve Ultra W & F - 1 8 2 6.7 17.0 2.3 10.7 Horsepower W & F - 182 0.0 0.7 10.0 4.3 XRM 5202 W & F - 182 3.7 12.0 0.3 6.1 6.7 0.7 4.8 Turf Builder + 22 3.7 Fertilized control2 0.0 2.0 4.3 2.6 10.6 8.8 NS 14.2 LSDo.os — i^i___ ‘These figures represent the area per plot covered by crabgrass. 2The 5 x 10 ft plots for these granular treatments were split into two 2 1/2 x 10 ft subplots. One-half of each material was applied to a dry and a wet subplot. 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 Table 10. Spurge counts1in turf treated on June 5 for the 1998 Postemergence Granular and Sprayable Broadleaf Weed Control Study. Material Spurge counts 1 0.0 Untreated control 4.0 2 Millenium Ultra 47.10% 3 Horsepower 50.40% 8.3 6.0 4 DTDA 47.30% 7.0 5 XRM 5202 55.80% 6 Confront 45.10% 4.3 3.7 7 Triplet (std) 49.67% 8 1.0 RDL202 89.76%DF 0.0 9 RDL211 55.98% 10 0.7 RDL414 55.48% MCPP (R) 51.90% 1.3 11 12 0.0 MCPP (O ) 25.96% 13 1.7 2,4-DP (R) 50.00% 14 2,4-DP (O) 25.00% 0.3 15 Dissolve (R) 57.70% 1.3 16 Dissolve (O) 43.31% 1.3 17 4.0 Super Trimec 3.0 18 Trimec Classic 3.4SL 1.0 19 Millenium Ultra W & F -182 4.7 Trupower W & F - 182 20 0.0 Dissolve W & F - 1 8 2 21 0.7 Dissolve Ultra W & F - 1 8 2 22 0.7 Horsepower W & F - 182 23 0.0 24 XRM 5202 W & F - 182 0.0 25 Turf Builder + 22 0.0 26 Fertilized control2 1.3 27 Millenium Ultra W & F -182 4.7 Trupower W & F - 182 28 0.0 29 Dissolve W & F - 1 8 2 0.0 Dissolve Ultra W & F - 1 8 2 30 0.0 Horsepower W & F - 182 31 1.3 XRM 5202 W & F - 182 32 0.0 Turf Builder + 22 33 0.0 34 Fertilized control2 4.4 LSDo.05 'These figures represent the number of spurge per plot. 2The 5 x 10 ft plots for these granular treatments were split into two 2 1/2 x 10 ft subplots. One-half of each material was applied to a dry and a wet subplot. 46 Tt a s 00 00 —< —h O »d OS O^ Os id id OS OS O' O' OS o s oo dONint^ONOOoorioô oo O' ^ vo o* oo *§ I a/2, < O^ oo 04 m «CS «es en o^ VO oo P O P VO «d VO vo m 04 m 04 fc -o o o o § £ p 1—1 p r f CO »—1 OS r—1 •d cd vd i—i 00 os ICS vo 00 o s r - en 3 p «o «es § ô 8 cCD 00 oo m vo Tf h- 000*h ”< N ^oo O vOO nK oo & *3 C o vo p cn o e n °® vo «d o id cd O^ Os OS ICS »es oo OS VO C4 O ' o o 04 e n OO ICS p OS OS Os O^ e n os OO OS O ' vo Os o s O ' "d0'0(Svo en lo O' vo p id vd vd vd vo O' O' oo 1—1 © O svooO O sO sO S ^ O' 3 & •o o o co O' O' r - en 0^ p OS o^ O^ en t}-* rfr m 04 04 lO vo O' ppppppppO'©OvTfO'Os3\ooTj- o d o o d c o ^ d o d > O .£ 3 C/2 OO .1 « oo 7 oo I Uh *5 00 oo ^ I i Pu Pu I Uh , I 04 2 SS tin « ^ cCO ^ > + D > ^ £ W CM e ^ £ » £ O 2 ^ 04 3 CD £ j> > o a w> -o o cd Uh tXQ TD O Q . U h o Oh 73 £ ON cK ¿0 ¿û ‘n Oh C/5 ”3 Uh 5 g £ 'cd Uh '3 Uh £ C/3 a U In i) S o a u Uh s s O 5u C/3 &• CO OX) oo ON 1 s I '3 Uh c _o O Oh *c3 e c^ c _o ’C 6 Oh £ Uh U h a u Uh B 8 o 0 17 22 14 0 5 39 0 9 2. Prograss EC+Sprint 330+46-0-0 .75 oz + 7.34 oz + .54 lb 19 38 60 14 79 62 3 +39 3. Prograss EC+Sprint 330+46-0-0 1.5 oz + 7.34 oz + .54 lb 53 74 82 0 35 73 18 +64 4. Primo EC+Sprint 330+46-0-0 .25 oz + 7.34 oz + .54 lb 11 22 14 39 24 60 5 0 5. Primo EC+Sprint 330+46-0-0 .5 oz + 7.34 oz + .54 lb 14 24 20 32 60 58 3 +26 6. Prograss EC+Sprint 330+46-0-0 .25 oz + 7.34 oz + .54 lb 13 17 17 6 13 49 5 +42 7. Prograss+Primo Sprint 330+46-0-0 .25 oz +.25 oz + 7.34 oz + .54 lb 15 24 11 7 63 55 10 +14 8. Prograss+Primo Sprint 330+46-0-0 .75 oz +.25 oz + 7.34oz + .54 lb 24 30 58 49 42 54 0 +55 9. Prograss+Primo Sprint 330+46-0-0 1.5 oz +.25 oz + 7.34 oz + .54 lb 60 90 79 54 95 69 38 +116 7.34 oz + .54 lb 19 19 28 19 36 46 3 +11 Q Treatment 1. Control 10. Sprint 330 + 46-0-0 11. Prograss Alone Rate/1000 fit2 O Q 28 1.5 oz 17 LSD 0.05 4 21 There were no reductions in quality o f fairway grasses at any of the sites during the season. 56 S h >> X +12 45 33 47 NS 12 58 Herbicide and Growth Regulator Studies Effect of Beacon on the Germination of Kentucky Bluegrass and Creeping Bentgrass - 1998 Report B arbara R. Bingaman, N ick E. Christians, M ichael B. Faust, and M elissa C. M cD ade In this study the effect o f CGA #136872 (Beacon) on seed germination and establishment o f Kentucky bluegrass and creeping bentgrass was evaluated. This study started in 1997 and concluded in 1998 at the Iowa State University Horticulture Research Station located north o f Ames, IA. The experimental plot was a bare soil area that had been tilled, raked, and prepared for seeding. The soil in this plot was a Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll) with an organic matter content o f 3.9%, a pH o f 7.0, 3 ppm P, and 76 ppm K. Individual plot size was 5 x 6 ft. There were three replications with 3 ft barrier rows between replications. CGA #136872 (Beacon) was applied at 20 and 40 g product/acre. Applications were made eight, four, and two weeks, and one day before seeding. The liquid materials were applied at 30 psi using a carbon dioxide backpack sprayer equipped with Teejet® #8006 flat fan nozzles. A methylated seed oil spreader (SCOIL MSO) was added at 0.25% V/V to all treatments except the control. CGA #136872 (Beacon) was mixed in 283 ml o f water per plot which translates to an application rate o f 3 gal/1000 ft2. Treatments were applied in 1997. The ‘eight weeks before seeding’ treatments were applied on July 14, the ‘four weeks before seeding’ on August 15, the ‘two weeks before seeding’ on August 28, and the ‘one day before seeding’ on September 11. Seeding took place on September 12, 1997. There was a heavy infestation o f weeds in the plots and border rows at the time o f seeding. All weeds were cut off with a hoe and removed. The soil from individual plots was not mixed with adjacent plots. Light raking was performed to make shallow grooves in the soil for the seeds. The plots were split into two 5 x 3 ft subplots. One o f these was seeded with ‘Penneagle’ creeping bentgrass at 1 lb/1000 ft2 and the other with ‘Award’ Kentucky bluegrass at 1.5 lb/1000 ft2. The seeding was done using a drop seeder. The plots were lightly raked following seeding to partially cover the seeds. Rainfall was sporadic during this period so the plot was irrigated daily. Bentgrass seedlings were first observed on September 18, 1997. Kentucky bluegrass germination was noted on September 26. By October 2, differences in bentgrass cover were beginning to appear but the plants were so small that data were not taken. Germination was determined as the percentage o f area per plot covered by each species. Percentage cover data were taken in 1997 on October 9, October 14, and November 11 (Table 1 and 2). Final 1997 data for this study were taken on November 11 because winter weather conditions had already set in. At this time, the bentgrass plants had matured but the bluegrass plants were still quite small. Additional germination data were taken spring 1998. Percentage creeping bentgrass cover data were taken on April 22 and May 13, 1998 (Table 3). Data for Kentucky bluegrass were taken on May 13. Data were analyzed with the Statistical Analysis System (SAS, version 6.12) and the Analysis o f Variance (ANOVA) procedure. Means were compared with Fisher’s Least Significant Difference (LSD) test. There were observable reductions in percentage cover o f creeping bentgrass on October 9 and 14, 1997 even though the differences were not significant. The November 11 data show that the ‘one day before seeding’ treatment of CGA #136872 (Beacon) at 40 g product/A significantly reduced the percentage cover o f bentgrass when compared with the untreated control. Although the ‘one day before seeding’ treatment o f CGA #136872 (Beacon) at 20 g product/A did numerically reduce the bentgrass cover, the percentage cover was not significantly reduced from the untreated control (Table 1). Because o f the weather conditions in October 1997, the bluegrass did not mature. The plants were still quite small when the final data were taken. There were significant differences (P > 0.06) in bluegrass cover on November 11. The percentage cover o f bluegrass treated with 20.0 g product/A at eight weeks, two weeks, and one day before seeding was significantly reduced when compared with the untreated control. In addition, significant reductions were recorded for bluegrass treated 2 weeks before seeding at 40 g product/A (Table 2). These were unusual results for the Kentucky bluegrass and observations will be made Spring 1998 to confirm these results. 57 Herbicide and Growth Regulator Studies In 1998, there were no significant reductions in either creeping bentgrass or Kentucky bluegrass percentage cover but there were numerical differences in bentgrass cover on both dates (Table 3). Percentage bentgrass cover was 10.0% on April 22 and 13.3% on May 13 in plots treated with CGA #136872 1 day before seeding as compared to 40.0 and 43.3% in the untreated plots. Kentucky bluegrass cover was similar in all untreated and treated plots in May 1998. Table 1. Percentage cover1 o f ‘Penneagle’ creeping bentgrass in the 1997 Carryover Seedling Study. Material2 Rate product/A. Timing o f application (before seeding) NA NA 8 weeks 4 weeks 2 weeks 1 day 8 weeks 4 weeks 2 weeks 1 day October 9 October 14 November 11 0 Mean cover / 32 35 55 41 2 0 .0 g 40 45 73 53 54 2 0 .0 g 43 43 75 20.0 g 30 32 72 44 20.0 g 22 27 40 29 33 65 44 40.0 g 35 40.0 g 20 20 45 28 40.0 g 20 23 53 32 40.0 g 5 5 12 7 NS NS 38 NS L S D o .05 'Percentage cover was estimated as the area per plot covered by creeping bentgrass. CGA #136872 was applied with SCOIL MSO at 0.25% VA/. 'Eight-weeks before seeding' materials were applied on July 14, '4-weeks' on August 15, '2-weeks' on August 28, and ' day' on September 11, 1997. Seeding took place on September 12, 1997. NS == means are not significantly different at the 0.05 level. 1 2 3 4 5 6 7 8 9 Untreated Control CGA #136872 CGA #136872 CGA #136872 CGA #136872 CGA #136872 CGA #136872 CGA #136872 CGA #136872 Table 2. Percentage cover1o f ‘Award’ Kentucky bluegrass in the 1997 0.06) 18 Mean % cover 27 20 28 17 19 27 22 21 20 NS NS NS LSD005 'Percentage cover was estimated as the area per plot covered by Kentucky bluegrass. 2CGA #136872 was applied with SCOIL MSO at 0.25% V/V. 'Eight-weeks before seeding' materials were applied on July 14, '4-weeks' on August 15, '2-weeks' on August 28, and Tday' on September 11, 1997. Seeding took place on September 12, 1997. NS = means are not significantly different at the 0.05 level. 58 Herbicide and Growth Regulator Studies Table 3. Spring 1998 percentage cover1 o f ‘Penneagle’ creeping bentgrass and ‘Award’ Kentucky bluegrass data for _________ the 1997 Carryover Seedling Study.______________________________________________________________ Creeping bentgrass____________ Material2 Rate product/A. Timing of application (before seeding) April 22 May 13 Mean cover Kentucky bluegrass May 13 o/_ NA 40.0 41.7 11.7 NA Untreated Control 43.3 53.3 11.7 CGA #136872 20.0 g 8 weeks 53.3 53.3 61.7 15.0 4 weeks 60.0 63.3 CGA #136872 20.0 g 20.0 g 2 weeks 60.0 56.7 11.7 CGA #136872 53.3 1 day 40.0 39.2 8.3 CGA #136872 20.0 g 38.3 40.0 g 53.3 53.3 53.3 6.7 8 weeks CGA #136872 30.8 8.3 4 weeks CGA #136872 40.0 g 28.3 33.3 CGA #136872 40.0 g 2 weeks 40.0 36.7 38.3 8.3 40.0 g 1 day 10.0 13.3 11.7 11.7 CGA #136872 NS NS NS NS LSDoos ‘Percentage cover was estimated as the area per plot covered by either creeping bentgrass or Kentucky bluegrass. 2CGA #136872 was applied with SCOIL MSO at 0.25% V/V. 'Eight-weeks before seeding' materials were applied on July 14, '4-weeks' on August 15, '2-weeks' on August 28, and '1day' on September 11, 1997. Seeding took place on September 12, 1997. NS = means are not significantly different at the 0.05 level. 1 2 3 4 5 6 7 8 9 59 Herbicide and Growth Regulator Studies Fairway Bentgrass Growth Regulator Study Barbara R. Bingaman, M elissa C. McDade, and Nick E. Christians 'Penneagle' Creeping bentgrass maintained at fairway height (0.5 inches) was evaluated after treatment with Proxy 2SL (ethephon). This study was conducted at the Iowa State University Horticulture Research Station located north o f Ames, IA. The study was arranged as a randomized complete block with 3 replications. Individual plot size was 5 x 5 ft with 3 ft barrier rows between replications. Chipco Proxy was applied at 0 ,4.7, 6.0, and 12.0 fl oz/1000 ft2 (Table 1). There were four applications at these rates made at four week intervals. The first application was made on June 22, 1998 on Penneagle established on a native (Nicollet) soil. Because o f construction in this area, the July, August, and September applications were made on a relocated plot of'Penneagle' established on a soil composed o f 1 part peat, 1 part Nicollet soil, and 1 part sand. Subsequent applications on the relocated site were made on July 23, August 20, and September 23. The Proxy was applied in 283 ml water/plot, which translates to an application rate o f 3 gal/1000 ft2. Applications were made at 30 psi with a carbon dioxide backpack sprayer equipped with Teejet® #8006 flat fan nozzles. Turf quality and color data were evaluated on a weekly basis following all applications and for six weeks following the last application. Turf quality was based on color, uniformity, and turf density and was based on a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. Visual observations o f phytotoxicity were made weekly following all applications and for 6 weeks following the last application. Phytotoxicity was recorded as either present or absent. Fresh clipping weights were taken on a weekly basis when there was sufficient growth. Mowing height for collecting clippings was 0.5 in. The data from the original and relocated plots were analyzed separately and are presented in discrete tables. All data were analyzed with the Statistical Analysis System (SAS, Version 6.12) and the Analysis o f Variance (ANOVA) procedure. Treatment effects were examined with Fisher's Least Significant Difference means separation test (LSD). Original Study Turf quality data were taken from the original plot on June 30, July 7, July 15, July 23, and July 30 (Table 1). Clippings were gathered on June 30, July 7, and July 30 (Table 2). On July 7, the bentgrass surfaces in the original plot were examined using a Clegg Impact Soil Tester that measured surface hardness. Readings of'hardness' were taken at 3 and 6 inch drop heights. Six readings per plot were taken and the highest and lowest readings were deleted. The remaining 4 values were averaged for each plot and each height. The means for each plot were analyzed (Table 3). The only significant differences in turf quality were recorded on July 15 (Table 1). All treated bentgrass had poorer quality than the untreated controls on July 15 but the quality was still above the lowest acceptable rating o f 6.0. There were no statistical differences in clipping weights on any o f the collection dates (Table 2). On July 7, the numerical differences between the treated and untreated bentgrass were quite large. The clipping weight o f turf treated with Proxy 2SL at 12.0 fl oz/1000 ft2 was almost twice that o f the untreated controls and Proxy at the lower rates produced at least a 150% increase in clippings as compared with untreated turf. Measurements with the Clegg Impact Soil Tester showed very little difference in hardness o f the bentgrass surface among the treatments (Table 3). Hardness was similar for treated and untreated turf at the 3 and 6 inch heights. Relocated Study Quality ratings were made for the relocated plot on August 5, August 12, August 20, August 27, September 4, September 11, September 17, September 23, October 2, October 9, October 15, October 22, and October 30 (Table 4-5). Clippings were taken from the relocated plot on August 12, September 4, September 11, September 23, and October 9 (Table 6). 60 Herbicide and Growth Regulator Studies There were no differences in visual quality between treated and untreated bentgrass until October 9 (Table 4-5). Significant reductions in quality were found in all treated bentgrass from October 9 through October 30. The quality o f the treated turf during this period did not drop below the lowest acceptable rating o f 6.0. Clipping weights were not significantly reduced by the treatments as compared with the untreated controls at any of the test dates (Table 6). Table 1. Visual quality1 of'Penneagle' creeping bentgrass treated in the 1998 Fairway Bentgrass Growth Regulator Study for June 30 through July 30 (on original site). Rate June July Material (fl oz/1000 ft2) 30 7 1 2 3 4 Untreated control Chipco Proxy 2SL Chipco Proxy 2SL Chipco Proxy 2SL N/A 4.7 6.0 12.0 9.0 7.0 7.0 7.0 8.0 8.0 8.0 8.0 July 15 July 23 July 30 Mean 9.0 7.7 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.4 7.7 7.8 7.8 _ _ _ 0.6 L S D o.o5 1Visual quality assessments were made using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. -- = Fisher's Least Significant Difference means separation test is not valid for these data. 0.1 Table 2. Fresh clipping weights1 o f ’Penneagle’ creeping bentgrass treated in the 1998 Fairway Bentgrass Growth Regulator Study (on original site). July 30 Mean weight 22.0 34.1 33.2 42.7 23.4 23.6 24.2 23.9 19.2 23.6 25.7 27.5 NS NS LSD 0 0 5 ‘Fresh clipping weights represent grams fresh tissue. Applications were made on June 22. -- = Fisher's Least Significant Difference means separation test is not valid for these data. NS NS Material Rate (fl oz/1000 ft2) June 30 July 7 rr 5 1 2 3 4 Untreated control Chipco Proxy 2SL Chipco Proxy 2SL Chipco Proxy 2SL N/A 4.7 6.0 12.0 12.1 13.0 19.7 15.8 Table 3. Hardness of'Penneagle' creeping bentgrass surfaces as measured with the Clegg Impact Soil Tester in the 1998 Fairway Bentgrass Growth Regulator Study (on original site). 3 inch Rate height Material (fl oz/1000 ft2) 1 2 3 4 Untreated control Chipco Proxy 2SL Chipco Proxy 2SL Chipco Proxy 2SL N/A 4.7 6.0 12.0 LSD 0 0 5 NS = means are not significantly different at the 0.05 level. 61 6 inch height 28.8 26.8 28.8 27.9 42.5 50.3 44.1 46.0 NS NS Herbicide and Growth Regulator Studies Table 4. Visual quality1 of'Penneagle' creeping bentgrass treated in the 1998 Fairway Bentgrass Growth Regulator 1 2 3 4 Study for August 5 - September 17 (on relocated site). Aug Aug Rate Material (fl oz/1000 ft2) 5 12 Aug 20 Aug 27 Sept 4 Sept 11 Sept 17 9.0 9.0 9.0 9.0 6.0 6.0 6.0 6.0 7.0 7.0 7.0 7.0 8.0 8.0 8.0 8.0 6.0 6.0 6.0 6.0 7.0 7.0 7.0 7.0 Untreated control Chipco Proxy 2SL Chipco Proxy 2SL Chipco Proxy 2SL N/A 4.7 6.0 12.0 9.0 9.0 9.0 9.0 ~ -~ L S D o.05 17777 Visual quality assessments were made using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. -- = Fisher's Least Significant Difference means separation test is not valid for these data. - Table 5. Visual quality1 of'Penneagle' creeping bentgrass treated in the 1998 Fairway Bentgrass Growth Regulator Study for September 23 - October 30 (on relocated site). Sept Oct Rate (fl oz/1000 ft2) 23 2 Material 1 2 3 4 Untreated control Chipco Proxy 2SL Chipco Proxy 2SL Chipco Proxy 2SL N/A 4.7 6.0 12.0 9.0 9.0 9.0 9.0 8.0 8.0 8.0 8.0 Oct 9 Oct 15 Oct 22 Oct 30 Mean 9.0 7.3 7.0 6.3 9.0 7.0 7.3 7.0 8.0 6.0 6.0 6.0 8.0 6.7 6.3 7.0 8.4 7.7 7.8 7.8 ___ ___ 0.7 1.7 0.6 1.0 LSDo.05 Visual quality assessments were made using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. —= Fisher's Least Significant Difference means separation test is not valid for these data. 0.1 Table 6. Fresh clipping weights1 of'Penneagle' creeping bentgrass treated in the 1998 Fairway Bentgrass Growth Regulator Study (on relocated site). Rate (fl oz/1000 ft2) Material 1 2 3 4 Untreated control Chipco Proxy 2SL Chipco Proxy 2SL Chipco Proxy 2SL N/A 4.7 6.0 12.0 Aug 12 Sept 4 Sept 11 Sept 23 Oct 9 Oct 30 Mean 1.0 1.4 1.4 1.6 63.7 62.3 76.7 98.3 25.3 30.0 26.0 42.0 rr fe 17.5 13.8 16.8 12.9 22.8 23.5 24.6 21.9 20.0 15.7 13.5 16.5 25.0 24.4 26.5 32.2 NS NS NS NS NS NS NS LSDo.05 ^resh clipping weights represent grams fresh tissue. Applications were made on July 23, August 20, and September 23. 62 Herbicide and Growth Regulator Studies Effect of Trinexapac-ethyl on Kentucky Bluegrass Sod Establishment Barbara R. Bingaman, Melissa C. McDade, Michael B. Faust, and NickE. Christians The growth regulator, Trinexapac-ethyl (Primo), was screened for effects on the establishment o f ‘Majestic’ Kentucky bluegrass sod. This study was conducted at the Iowa State University Horticulture Research Station north o f Ames, Iowa in a ‘Majestic’ Kentucky bluegrass area where a similar sod study was conducted in 1996. The sod was cut in the opposite direction as the previous study to offset any possible carryover effects. The soil in this area was a Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll) with 4.5% organic matter, a pH o f 7.12, 4 ppm P, and 93 ppm K. Rainfall was sporadic throughout the duration o f this study. Supplemental irrigation was used to maintain the bluegrass in good growing condition and to facilitate sod root development. Individual plots were 5 x 5 ft and four replications were conducted. There were six Primo treatment regimes and an untreated control. All Primo applications were made at the label rate for Kentucky bluegrass (0.75 fl oz/1000 ft2). The timing o f applications was in reference to sod cutting and establishment. The treatments included an application o f Primo six and two weeks before sod establishment, two and six weeks after, two weeks before, two weeks after, six and two weeks before plus two and six weeks after, and two weeks before plus two weeks after (Table 1). Primo 1EC was applied at 30 psi with a carbon dioxide powered backpack sprayer equipped with Teejet® #8006 flat fan nozzles. The Primo was mixed in 283 ml o f water which translates to an application rate o f 3 gal/1000 ft2. The six weeks before sod establishment treatments were applied on June 10, 1998 and the two weeks before were made on July 9. The two weeks after treatments were applied on August 5 and the six weeks after sod establishment treatments were made on September 2. The bluegrass on the entire experimental plot was cut on July 23 using an 18-inch sod cutter. Within each individual plot, sod pieces were cut that matched the outside diameter of 12 x 12 in wooden frames that were constructed with bottoms o f 18 mesh screen. The pieces were trimmed and transplanted into the frames and the frames with the sod pieces were returned to the holes and placed flush with the soil surface. There were four frames per individual plot, one in each o f four quadrants. The study was watered thoroughly upon completion and was watered on a regular basis to prevent the sod from drying. Root development was measured using a hydraulic sod pulling apparatus equipped with steel cables that could be attached to screw hooks on the corners o f wooden frames (Figure 1). The tensile strength required to ‘pull’ a frame from the soil was measured in pounds per square inch (psi). One frame per plot was sampled on each o f four collection dates beginning on August 4, two weeks after sod establishment. The other frames were harvested at twoweek intervals on August 18, September 2, and September 16 (Table 4). Visual quality data were taken on a weekly basis from June 16 through September 16 (Tables 2 and 3). Visual quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. Data were analyzed using the Statistical Analysis System (SAS, Version 6.12) and the Analysis o f Variance (ANOVA) procedure. Means were compared with Fisher’s Least Significant Difference (LSD) means separation test. On June 24 and July 17, numerical differences in visual quality were found. The Primo treated plots showed a slight phytotoxicity on June 24, two weeks after treatment. On July 17, the bluegrass that was treated on both June 10 and July 9 (treatments 2 and 6) had a better color than the other treated and untreated turf. The bluegrass treated on July 9 only (treatments 4 and 7) showed a slight phytotoxicity. The phytotoxicity was not detectable after a few days. There were no differences in turf quality after July 17 (Table 2). 63 Herbicide and Growth Regulator Studies There were numerical tensile strength differences in sod harvested on August 4, August 18, and September 2 but the treatment effects were not statistically different (Table 4). On September 16, the sod treated with Primo 1EC two weeks before establishment had significantly more root development than sod treated two and six weeks before, two weeks before and after, sod treated two and six weeks after, and the untreated control. Root development was similar for sod treated six and two weeks before and two and six weeks after establishment and sod treated two weeks after establishment. The data from September 16 show that root tensile strength was 30% higher for sod treated with Primo 1EC two weeks before establishment than for untreated sod. Table 1. Timing o f Primo applications for the 1998 Sod Establishment Study Timing o f applications* 6 weeks 2 weeks 2 weeks 6 weeks Material Rate /plot before before after after 1 Untreated control NA NA NA NA NA 2 0.75 oz yes Primo 1EC yes no no 3 0.75 oz no no Primo 1EC yes yes 4 0.75 oz no Primo 1EC yes no no 0.75 oz 5 Primo 1EC no no yes no 6 0.75 oz Primo 1EC yes yes yes yes 7 0.75 oz Primo 1EC no yes yes no *Timing is in reference to cutting of sod. Six and two weeks before cutting and two and six weeks after establishment. Sod Harvested on July 23. Six weeks before harvest applications made June 10, 2 weeks before on July 9, 2 weeks after on August 5, and 6 weeks after on September 2. Frames were pulled on August 4, August 18, September 2, and September 16. Table 2. Visual quality1 o f Kentucky bluegrass in the 1998 Sod Establishment Study (June 16 - August 4). June June July July June July August Material 16 24 30 7 17 4 29 1 Untreated control 8.0 8.0 7.0 7.0 6.3 7.0 7.0 2 8.0 7.0 7.5 5.0 7.0 7.0 Primo 1EC 7.0 8.0 8.0 7.0 3 Primo 1EC 7.0 6.3 7.0 7.0 4 8.0 6.0 7.0 6.0 Primo 1EC 7.0 7.0 7.0 5 Primo 1EC 8.0 8.0 7.0 7.0 6.5 7.0 7.0 6 Primo 1EC 8.0 5.0 7.0 7.0 6.8 7.0 7.0 7 Primo 1EC 8.0 8.0 7.0 7.0 6.0 7.0 7.0 NS NS ~ -~ LSD0.05 11Visual wquality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. NS = means are not significantly different at the 0.05 level. —= means comparison tests are not applicable to these data. Table 3. Visual quality1 o f Kentucky bluegrass in the 1998 Sod Establishment Study (August 11 - September 16). Sept Sept August August August Sept Mean 11 Material 18 26 2 9 16 quality 1 Untreated control 7.0 8.0 7.0 7.0 8.0 9.0 7.4 2 8.0 8.0 Primo 1EC 7.0 7.0 7.0 9.0 7.3 3 Primo 1EC 7.0 7.0 8.0 8.0 7.0 9.0 7.4 4 Primo 1EC 7.0 7.0 7.0 8.0 8.0 9.0 7.2 5 7.0 8.0 Primo 1EC 7.0 7.0 8.0 9.0 7.4 6 Primo 1EC 7.0 8.0 8.0 7.0 7.0 9.0 7.2 7 Primo 1EC 7.0 7.0 7.0 8.0 8.0 9.0 7.4 0.1 LSD 0.05 I77T Visual quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. NS = means are not significantly different at the 0.05 level. ~ = means comparison tests are not applicable to these data. 64 Herbicide and Growth Regulator Studies Table 4. Root tensile strength and knitting o f Kentucky bluegrass sod growing in frames in the 1998 Sod _________ Establishment Study as measured by the number of pounds per square inch (psi) to pull 1 ft2 frames. August August September September Mean __________ Material______________ 4______________ 18______________ 2______________ 16___________ strength 1 2 3 4 5 6 7 Untreated control Primo 1EC Primo 1EC Primo 1EC Primo 1EC Primo 1EC Primo 1EC LSDo.os 57.5 37.5 53.8 18.8 23.8 35.0 33.8 NS 252.5 285.0 270.0 298.8 272.5 315.0 243.8 NS --------- p si-------385.0 395.0 300.0 346.3 341.3 331.3 346.3 NS 355.0 385.0 382.5 460.0 401.3 415.0 395.0 63.0 P > F = 0.07 NS = means are not significantly different at the 0.05 level. Frames were pulled on August 4, August 18, September 2, and September 16. Figure 1. The hydraulic sod pulling device used to measure root tensile strength o f the sod in the 1998 Sod Establishment Study. 262.5 275.6 251.6 280.9 259.7 274.1 254.7 NS Herbicide and Growth Regulator Studies Effects of Trinexapac-ethyl on an n u a Populations in Green Height Creeping Bentgrass B arbara R. Bingaman, M elissa C. M cDade, an d N ick E. C hristians This study was designed to evaluate the growth regulator, Trinexapac-ethyl (Primo), for Poa annua control in creeping bentgrass maintained at green height following a fall conversion program. This study was conducted on a practice green at Veenker Memorial Golf Course in Ames, IA. The turf in this area consisted o f creeping bentgrass with an infestation o f Poa annua that ranged from 50 to 80% through the 1998 season. The experimental design was a randomized complete block. Individual plot size was 5 x 5 ft with 3 replications. Treatments were applied to the same plots as in 1997. Primo 1EC was used at 0.3 fl oz/1000 ft2 monthly from May through September. Initial application was made on May 14 and subsequent applications were made on June 17, July 14, August 19, and September 23, 1998. Primo 1EC was mixed with 283 ml o f water (3 gal/1000 ft2) and was applied at 30 psi with a carbon dioxide backpack sprayer equipped with Teejet® #8006 flat fan nozzles. All applications were made between 6:30 and 7:00 a.m. Following applications, the plot was watered with the normal watering schedule in the late afternoon. Visual turf quality data were taken from April 22 through October 27, 1998 (Tables 1 and 2). Visual turf quality was assessed with a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst turf quality. On each o f these dates, the plots were checked for phytotoxicity. Phytotoxicity was assessed using a 9 to 1 scale with 9 = no damage, 6 = moderate tip bum (browning) and 4 = severe tip bum. Poa annua populations were estimated as the percentage o f area per plot covered by Poa annua. Percentage cover data were taken on May 13, May 28, June 12, July 14, July 30, August 12, August 26, September 10, September 22, October 1, October 14, and October 27 (Table 3 and 4). Poa annua control is represented by calculating reductions in percentage cover as compared with the untreated control (Tables 5 and 6). On May 16, 1998, the practice green was core aerified and topdressed. The effects o f Primo on the recovery from this procedure were examined on May 28 by estimating the percentage bentgrass recovery. Estimations were based on bentgrass growth into the 'core areas'. All data were analyzed with the Statistical Analysis System (SAS, Version 6.12) and the Analysis o f Variance (ANOVA) procedure. Treatment effects were compared with Fisher's Least Significant Difference (LSD) test. In general, quality differences between the treated and untreated bentgrass were found throughout the season approximately 14 days following Primo applications (Tables 1 and 2). In October, treated bentgrass consistently was darker green than the untreated bentgrass. No phytotoxicity was found. Primo treated bentgrass had less Poa annua than the untreated control from May 28 through October 27 (Tables 4 and 5). The differences in percentage cover were significant on all data collection dates except July 30 and August 26. Mean percentage Poa annua cover also was significantly lower in treated bentgrass than untreated. Significant reductions in Poa annua cover ranged from 22.7 to 63.3% as compared with the untreated controls (Tables 5 and 6). The mean reduction in Primo treated bentgrass was 47.6%. Primo treated bentgrass recovered from core aerification significantly slower that untreated bentgrass (P > F = 0.0339). On May 28, recovery in the Primo treated bentgrass was 65% as compared with 88.3% recovery for untreated bentgrass. By June 12, recovery was 100% for both treated and untreated bentgrass. Additional percentage cover data will be taken spring 1999 beginning at greenup. Winter damage also will be assessed spring 1999. 66 Herbicide and Growth Regulator Studies Table 1. Visual quality1 o f green height creeping bentgrass treated for the 1997-98 Green Conversion Study (April 22 - August 12, 1998). Rate fl otJ A p r il May June June Material May 22 30 1000 ft2 28 12 13 N /A 6.0 7.0 6.7 7.0 1 Untreated control 6.7 8.0 0.3 6.0 7.0 8.0 2 Primo 1EC2 7.0 1.4 NS NS L S D o.os Height Poa annua July 14 7.0 7.0 - July 30 7.0 7.0 - Aug 12 7.0 7.0 - ‘Visual quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. 2Primo 1EC applications were made on May 14, June 17, July 14, August 19, and September 23. NS = Means are not significantly different at the 0.05 level. — = Means separation tests are not valid for these data. Table 2. Visual quality1 o f green height creeping bentgrass treated 1998 Green Height Poa annua Conversion Material 1 Untreated control 2 Primo2 L S D o os Ur:...., — Rate fl oz/ 1000 ft2 N/A 0.3 - ________ ^ .. Aug 26 7.0 8.0 - Sept 10 7.0 7.0 - Oct 1 7.0 9.0 - Sept 22 7.0 7.0 - Oct 14 7.0 9.0 - Oct 27 6.3 9.0 1.4 Mean quality 6.8 7.6 0.5 Primo applications were made on May 14, June 17, July 14, August 19, and September 23. NS = Means are not significantly different at the 0.05 level. — = Means separation tests are not valid for these data. Table 3. Percentage Poa annua cover1 in creeping bentgrass treated for the 1997-98 Green Height Poa annua Conversion Study (May 13 - August ' — n — ~ 26, 1998). j Rate July July August August May June May Material fl oz/ 12 14 30 26 12 13 28 1000 ft2 0 /n 1 Untreated control 2 Primo2 N/A 0.3 46.7 53.3 NS 73.3 56.7 7.2 L S D o os " T t l _______ __________ _____ L~ ‘These values represent the area per plot covered by Poa annua. 58.3 21.7 28.7 78.3 43.3 12.4 51.7 18.3 NS 46.7 20.0 28.7 50.0 25.0 NS 2Primo applications were made on May 14, June 17, July 14, August 19, and September 23. NS = Means are not significantly different at the 0.05 level. Table 4. Percentage Poa annua cover1 in creeping bentgrass treated for the 1997-98 Green Height Poa annua _________ Conversion Study (September 10 - October 27, 1998).________________________________________ Rate Oct Mean Oct Oct Sept fl oz/ Sept Material Cover 27 14 1 10 1000 ft2 22 OA N/A 0.3 43.3 23.3 12.4 46.7 21.7 12.4 LSD 005 1^1_____ 1__________ _ area per plot covered by Poa annua. ‘These values represent. the 1 Untreated control 2 Primo2 50.0 18.3 7.2 53.3 21.7 28.7 2Primo applications were made on May 14, June 17, July 14, August 19, and September 23. 67 63.3 23.3 32.9 46.7 20.0 7.2 Herbicide and Growth Regulator Studies Table 5. Reductions in percentage Poa annua cover1 in creeping bentgrass treated for the 1997-98 Green Height _________ Poa annua Conversion Study (May 13 - August 26, 1998).______________________________________ Rate June July July August Material fl oz/ May May August 1000 ft2 12 14 30 12 13 28 26 % 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 22.7 44.7 62.8 64.5 57.2 50.0 NS 9.8 15.9 17.8 18.5 20.5 24.8 L S D o os ‘These values represent reductions in the area per plot covered by Poa annua ;as compared with the untreated controls. Primo applications were made on May 14, June 17, July 14, August 19, and September 23. NS = Means are not significantly different at the 0.05 level. 1 Untreated control 2 Primo2 N/A 0.3 Table 6. Reductions in percentage Poa annua cover1in creeping bentgrass treated for the 1997-98 Green Height Poa annua Conversion Study (September 10 - October 27, 1998). Rate fl oz/ Sept Material Oct Oct Oct Sept Mean 1000 ft2 10 22 1 14 27 Cover % 0.0 0.0 0.0 0.0 46.1 53.6 63.3 59.3 28.7 14.3 27.0 26.6 LSD o.05 ‘These values represent reductions in the area per plot covered by Poa annua as compared with the 2Primo applications were made on May 14, June 17, July 14, August 19, and September 23. 1 Untreated control 2 Primo2 N/A 0.3 68 0.0 0.0 63.1 47.6 30.0 13.2 untreated controls. Herbicide and Growth Regulator Studies Effects of Trinexapac-ethyl on an nu a Populations in Fairway Height Creeping Bentgrass Barbara R. Bingaman, Melissa C. McDade, and Nick E. Christians The growth regulator, Trinexapac-ethyl (Primo), was evaluated for P o a an n u a control in creeping bentgrass maintained at fairway height following a fall conversion program in this study. It was conducted on a fairway height area surrounding a practice green at Veenker Memorial Golf Course in Ames, IA. The turf in this area consisted of perennial ryegrass with an infestation o f P o a annua that ranged from 20 to 0% through the 1998 season. The experimental design was a randomized complete block. Individual plot size was 5 x 5 ft with 3 replications. The plot was overseeded with perennial ryegrass in September 1998. Treatments were applied to the same plots as in 1997. Primo 1EC was used at 0.5 fl oz/1000 ft2 monthly from May through September. Initial application was made on May 14 and subsequent applications were made on June 17, July 14, August 19, and September 23, 1998. Primo 1EC was mixed with 283 ml o f water (3 gal/1000 ft2) and was applied at 30 psi with a C 0 2 backpack sprayer equipped with Teejet® #8006 Teeject flat fan nozzles. All applications were made between 6:30 and 7:00 a.m. Following applications, the plot was watered with the normal watering schedule in the late afternoon. Visual turf quality data were taken from April 22 through October 27 (Tables 1 and 2). Visual turf quality was assessed with a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst turf quality. On each o f these dates, the plots were checked for phytotoxicity. Phytotoxicity was assessed using a 9 to 1 scale with 9 = no damage, 6 = moderate tip bum (browning) and 4 = severe tip bum. All data were analyzed with the Statistical Analysis System (SAS, Version 6.12) and the Analysis o f Variance (ANOVA) procedure. Treatment effects were examined with Fisher's Least Significant Difference means separation test (LSD). P o a an n u a populations were estimated as the percentage o f area per plot covered by P o a annua. Percentage cover data were taken on May 13, May 28, June 12, June 30, July 14, July 30, August 12, August 26, September 10, September 22, October 1, October 14, and October 27 (Tables 3 and 4). Additional percentage cover data will be taken spring 1999 beginning at greenup. Winter damage also will be assessed spring 1999. Primo treated ryegrass had a darker green color than the untreated ryegrass on May 28 and June 12 approximately 2 4 weeks following the first Primo application (Table 1). No further quality differences were noted through the season until October 27 (Table 2). No phytotoxicity was found. Primo treated ryegrass had less P o a annua than the untreated control from May 13 through July 30 (Tables 3 and 4). The reduction in P o a an n u a was significantly different on May 13. The reductions were numerically different from May 28 through July 30. By August 19, the P o a annua had all died in the experimental plot. Close examinations o f the plot were made from August 19 through October 27 and no P o a annua was found. 69 Herbicide and Growth Regulator Studies Table 1. Visual quality1 o f perennial ryegrass treated for the 1997-98 Fairway Height P o a a n n u a Conversion Study (April 22 - August 12, 1998). Rate fl oz/ April May May June June July July Material 1000 ft2 22 12 30 14 30 13 28 N/A 9.0 7.3 9.0 9.0 9.0 1 Untreated control 9.0 7.3 9.0 0.5 9.0 9.0 8.7 9.0 9.0 8.7 2 Primo 1EC2 1.4 1.4 L SD oos ‘Visual quality was assessed using a 9 to 1 scale with 9 - best, 6 = lowest acceptable, and 1 = worst quality. 2Primo 1EC applications were made on May 14, June 17, July 14, August 19, and September 23. —= Means separation tests are not valid for these data. Aug 12 9.0 9.0 - Table 2. Visual quality1 o f perennial ryegrass treated 1998 Fairway Height P o a an n u a Conversion Study (August 26 - October 27, 1998). Rate fl oz/ Aug Oct Oct Material Sept Sept Oct Mean 1000 ft2 26 14 10 22 1 27 quality 9.0 N/A 9.0 9.0 7.0 9.0 1 Untreated control 9.0 8.6 0.5 9.0 9.0 9.0 9.0 2 Primo2 9.0 9.0 8.9 0.2 LSDoos " T T T :----------;------------ 7 7 ---------------------------------- ‘Visual quality was assessed using a 9 to 1 scale with 9 - best, 6 = lowest acceptable, and 1 = worst quality. 2Primo applications were made on May 14, June 17, July 14, August 19, and September 23. — = Means separation tests are not valid for these data. Table 3. Percentage P o a an n u a cover1 in perennial ryegrass treated for the 1997-98 Fairway Height P o a an n ua _________ Conversion Study (May 13 - August 26, 1998).__________________________________________________ Rate Material fl oz/ May May June June July July Aug Aug 1000 ft2 13 28 12 30 14 30 19 26 % 63.3 16.0 15.0 33.3 5.0 3.7 24.8 NS NS L SD oos ‘These values represent the area per plot covered by P o a annua. 2Primo applications were made on May 14, June 17, July 14, August NS = Means are not significantly different at the 0.05 level. —= Means separation tests are not valid for these data. 1 Untreated control 2 Primo2 N/A 0.5 3.7 1.7 NS 0.7 0.3 NS 0.7 0.3 NS 0.0 0.0 - 0.0 0.0 - 19, and September 23. Table 4. Percentage P o a an n u a cover1 in perennial ryegrass treated for the 1997-98 Fairway Height P o a an n ua _________ Conversion Study (September 10 - October 27, 1998).___________ ________________________________ Rate fl oz/ Sept Sept Oct Oct Oct Mean __________ Material__________1000 ft2 10_________ 22_________ 1__________14_________27 Cover ------------------------ % -----------------------1 Untreated control N/A 0.0 0.0 0.0 0.0 2 Primo2 0.5 0.0 0.0 0.0 0.0 LSD q.05 — — ‘These values represent the area per plot covered by P o a annua. 2Primo applications were made on May 14, June 17, July 14, August 19, and September 23. -- = Means separation tests are not valid for these data. 70 0.0 0.0 — 8.3 3.7 NS Turfgrass Disease Research Evaluation o f Fungicides for C ontrol o f D ollar Spot on C reeping Bentgrass - 1998 Mark L. Gleason, Nick E. Christians, and James R. Dickson Trials were conducted at the Iowa State University Horticulture Research Farm near Gilbert, Iowa. Fungicides were applied to ‘Penncross’ creeping bentgrass maintained at 5/32-inch cutting height, using a modified bicycle sprayer at 30 psi and a dilution rate o f 5 gal/1,000 ft2. The experimental design was a randomized complete block with four replications. All plots measured 4 ft x 5 ft. Five days after inoculation o f the entire plot with rye grain infested with the dollar spot pathogen, fungicide applications began on Junel2. Subsequent applications were made on June 19, June 26 and on July 3, 10, 17, and 24. Dollar spot symptoms were first observed on June 26. Disease development on untreated check plots was light on 29 Jun and moderately severe on July 10 and 16. Most, but not all, fungicide treatments exhibited significantly (LSD, P=0.05) less disease than the untreated check. Phytotoxicity (browning o f tips o f grass blades) on plots treated with AMY 300 became progressively worse during July, and was more severe at the 2 oz than the 1 oz rate. Table 1. 1998 Dollar Spot Trial at ISU Horticulture Station._______________ ________________________ Interval Trt Product (days) Rate/1,000 ft2 1 Mean % Plot Diseased June 29 July 10 August 25 -- 4.0 17.5 22.5 14 0.0 0.0 0.0 Chipco 26 GT 2 oz + Cleary’s 3336 F 2 fl oz 3 Cleary 3336 50 WP 2 oz 14 0.0 0.0 0.0 4 Spectro 90 WDG 4 oz 14 0.0 0.0 0.0 5 Spectro 90 WDG 8 oz 14 1.5 0.0 0.0 6 WAC 75 5 oz 14 0.0 0.0 0.0 7 WAC 76 5.3 oz 14 0.0 0.0 0.0 8 Thalonil 90 DF 3.5 oz 14 2.2 2.8 4.0 9 TRA-0232 3.5 oz 14 2.1 4.8 5.8 10 BAS 505 50 DF 0.11 oz 14 0.2 0.3 0.0 11 BAS 505 50 DF 0.14 oz 14 0.3 0.1 0.3 12 BAS 505 50 DF 0.18 oz 14 0.1 0.0 0.0 13 BAS 505 50 DF 0.18 oz 28 0.0 1.5 1.9 14 BAS 505 50 DF 0.26 oz 28 0.1 1.1 1.2 15 BAS 510 4.17 SC 0.11 fl oz 14 2.8 13.0 14.5 16 BAS 510 4.17 SC 0.14 fl oz 14 2.4 0.4 0.2 17 BAS 510 4.17 SC 0.18 fl oz 14 4.1 3.3 1.8 18 BAS 510 4.17 SC 0.18 fl oz 28 3.6 18.0 20.8 19 BAS 510 4.17 SC 0.26 fl oz 28 1.5 8.0 9.0 20 Banner MAXX 1.24 MC 1.0 fl oz 21 0.0 0.0 0.0 + Thalonil 90 DF 6 oz Heritage 50 WP 0.2 oz 14 2.3 3.3 3.7 + Daconil Ultrex 3.8 oz 2 21 71 Turfgrass Disease Research Interval Trt Product Rate/l ,000 ft2 22 Daconil Ultrex 3.8 oz 14 23 Eagle 50 WP 0.5 oz 14 Daconil Ultrex 3.8 oz 14 24 Eagle 50 WP 1.2 oz 25 ZeroTol 0.5 fl oz/80 fit2 26 Bayleton 50 DF 0.25 oz + Daconil Ultrex 1.82 oz Lynx 45 WP 0.28 oz + Daconil Ultrex 1.82 oz 28 Bayleton 50 DF 29 (days) Mean % Plot Diseased June 29 July 10 August 25 2.0 3.3 8.3 1.1 0.0 0.0 28 0.5 3.1 2.0 7 4.0 18.3 21.3 14 2.4 0.3 0.3 14 0.9 0.1 0.0 0.25 oz 14 1.3 0.7 0.3 Lynx 45 WP 0.28 oz 14 0.3 0.1 0.0 30 A M V 300 1 oz 14 3.8 12.5 18.8 31 A M V 300 2 oz 14 3.5 17.5 20.5 32 UCC-A1562 20 SC 1 fl oz 21 1.0 0.3 2.3 33 UCC-A1562 20 SC 4 fl oz 21 0.1 0.3 0.0 34 UCC-A1562 20 SC 8 fl oz 21 0.0 0.0 0.0 35 Heritage 50 WG 0.4 oz 21 1.4 0.0 4.1 1.8 8.9 8.4 4th and later sprays: 27 Least Significant Difference3 aP = 0.05. n = 4 replications. 72 Turfgrass Disease Research Evaluation of Fungicides for Control of Pythium Blight on Perennial Ryegrass - 1998 Mark L. Gleason Trials were conducted on perennial ryegrass, maintained at fairway height, at the Turfgrass Research Area o f the ISU Horticulture Research Farm near Gilbert, IA. The entire plot was inoculated on June 23 and August 7 with rye grain infested with isolates o f Pythium aphanidermatum. NOTE: The grain used for the June 23 inoculation was infested with two isolates o f P. aphanidermatum, one o f which was resistant to metalaxyl. The grain used for the August 7 inoculation was infested with four isolates o f P. aphanidermatum, two o f which were resistant to metalaxyl. Immediately after the infested grain was applied, the inoculated area was covered by Seedguard geotextile fabric for several days (June 23-24 and August 7-10). Fungicide treatments were applied after the cover was removed. Fungicides were applied with a modified bicycle sprayer at 30 psi and a dilution rate of 5 gal/1,000 ft2. Fungicide applications after the first inoculation were made on June 25, then repeated at specified intervals on July 6, 9, 16, and 23. A single fungicide application, on August 14, was made after the second inoculation. Weather conditions were conducive to Pythium blight during several periods after the June inoculation, but disease pressure was slight during June and July. Pythium blight symptoms appeared two weeks after the second inoculation. Disease development on August 25 was moderate. Ten treatments had significantly (LSD, P=0.05) less disease than the untreated check. No phytotoxicity was observed in any o f the plots. Table 1. 1998 Pythium blight trial on perennial ryegrass (fairway height). Trt Product 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 BAS 500 2.09 EC Subdue MAXX Subdue MAXX B and 6 EC Aliette Signature Heritage 50 WG Subdue MAXX +Banol Subdue MAXX + Aliette Signature Subdue MAXX + Aliette Signature Heritage 50 WG Heritage 50 WG Heritage 50 WG Banol 6 EC B and 6 EC + Fore FF B an d 6 EC + Heritage 50 WG Subdue MAXX + Fore F Subdue MAXX + Heritage 50 WG Aliette Signature 80 WP S-8172 S-8174 + S-5223 S-7248 S-7248 UCC-A1562 20 SC UCC-A1562 20 SC UCC-A1562 20 SC Least Significant Difference3 Rate/1,000 ft2 — 0.7 fl oz 1 fl oz 0.5 fl oz 1.6 oz 4.0 oz 0.2 oz 0.5 oz 1.6 oz 0.5 oz 4.0 oz 0.5 oz 2.0 oz 0.4 oz 0.4 oz 0.7 oz 2 fl oz 1 fl oz 6.4 fl oz 1 fl oz 0.2 oz 0.5 oz 6.4 fl oz 0.4 fl oz 0.2 fl oz 8 oz 6 fl oz 2 fl oz 4.5 oz (add 1st) 20.5 oz 41 oz 1 fl oz 4 fl oz 8 fl oz aP = 0.05. n = 4 replications 73 Interval (days) — 14 14 14 14 14 14 14 Mean number o f infection centers June 29 July 10 August 25 0.0 0.0 8.75 0.0 0.0 3.75 0.0 0.0 0.0 0.0 0.0 0.0 0.0 14 0.0 0.25 0.0 14 0.0 0.0 0.0 10 14 14 14 14 0.0 0.0 0.0 2.5 4.5 4.0 0.0 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 5.0 14 0.0 0.0 2.75 14 0.25 0.0 7.25 14 14 14 0.0 0.0 0.25 0.0 0.0 1.0 0.75 0.25 0.5 14 14 14 14 14 0.0 0.0 0.0 0.0 0.25 0.25 0.25 0.25 0.0 0.0 0.38 0.34 0.5 1.5 0.5 5.25 8.5 7.14 0.25 0.25 0.0 0.0 0.0 0.0 0.0 1.0 1.25 0.0 0.75 2.5 1.0 1.0 Turfgrass Disease Research Evaluation o f Fungicides for C ontrol o f Brown Patch in C reeping B entgrass - 1998 MarkL. Gleason Trials were conducted at Veenker Memorial Golf Course on the campus o f Iowa State University. Fungicides were applied to creeping bentgrass maintained at 5/32-inch cutting height, using a modified bicycle sprayer at 30 psi and a dilution rate o f 5 gal/1,000 ft2. The experimental design was a randomized complete block with four replications. All plots measured 4 ft x 5 ft. All plots were bordered on opposite sides by 1-fit-wide strips o f untreated turf in order to help create uniform disease pressure. Fungicide applications began on May 22 (Bio-Trek treatments only). The remainder o f the treatments received the first application on June 10. Subsequent applications were made on June 17, 19, and 24; and July 1, 8, 15, and 22. Brown patch symptoms were first observed on June 29. Disease development on untreated check plots - expressed on a 0-5 scale (0=no disease, 1=1=5%, 2=5-10%, 3=10-25%, 4=25-50%, 5=>50%) - was light on June 29 and moderate on July 10 and 22. Most, but not all, fungicide treatments exhibited significantly (LSD, P=0.05) less disease than the untreated check. Phytotoxicity (browning o f tips o f grass blades) on plots treated with AMV 300 became progressively worse during July, and was more severe at the higher rate o f product. Table 1, 1998 Brown Patch Trial - WOI creeping bentgrass greens, Veenker Memorial Golf Course, ISU. Interval Trt 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 Product Bio-Trek G Bio-Trek G Bio-Trek WP Heritage Bio-Trek G Bio-Trek G Bio-Trek WP Heritage 3336 50 WP Spectro 90 WDG WAC 75 WAC 76 Thalonil 90 DF TRA-0232 Thalonil 90 DF TRA-0232 BAS 500 2.09 EC BAS 505 DF CGA 279202 50 WG CGA 279202 50 WG CGA 279202 50 WG + Banner MAXX Chipco 26 GT 2 SC EXP 10830A 4.17 SC* EXP 10830A 4.17 SC* Rate/1,000 ft2 1.5 lb 1.5 lb 6 oz 0.2 oz 1.5 lb 1.5 lb 3.0 oz 0.2 oz 4 oz 8 oz 5 oz 5.3 oz 3.5 oz 3.5 oz 3.5 oz 3.5 oz 0.42 fl oz 0.21 oz 0.1 oz 0.15 oz 0.1 oz 1 fl oz 4 fl oz 0.3 fl oz 0.6 fl oz (days) — late May 4 wks later 10 days later 21 late May 4 wks later 10 days later 21 days 14 14 14 14 7 7 14 14 14 14 14 14 14 14 14 14 74 Mean disease rating3 June 29 1.75 1.25 July 10 3.25 0.00 July 22 2.50 0.25 0.25 0.25 0.00 0.25 0.00 0.25 0.00 0.00 0.25 0.00 0.25 0.50 1.00 0.25 0.00 0.50 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.25 0.25 0.25 0.25 0.00 0.50 0.00 0.00 0.00 0.00 0.00 0.75 0.00 0.25 0.25 0.00 0.50 0.50 0.25 1.00 0.50 0.00 0.00 1.75 0.00 0.25 0.50 0.25 Turfgrass Disease Research Trt 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 Product EXP 10830A 4.17 SC* Chipco Triton 1.67 SC (=80318) Chipco Triton 1.67 SC Heritage 50 WG Heritage 50 WG +Daconil Ultrex Heritage 50 WG ALTERNATE WITH Daconil Ultrex Eagle 50 WP + Fore 75 DG ZeroTol ProStar 50 WP ProStar 70 WP ProStar 70 WP + Heritage 50 WG S-8172 S-8172 S-8206 S-8206 AMV 300 AMV 300 UCC-A1562 20 SC UCC-A1562 20 SC UCC-A1562 20 SC Least Significant Difference13 Rate/1,000 ft2 1 fl oz 1 fl oz 1 fl oz 0.2 oz 0.2 oz 3.8 oz 0.4 oz 3.8 oz 0.6 oz 6 oz 0.5 fl oz/80 ft2 3 oz 2.25 oz 1.5 oz 0.2 oz 4 fl oz 8 fl oz 3.2 fl oz 6.4 fl oz 1 fl oz 2 fl oz 1 fl oz 4 fl oz 8 fl oz Interval (days) 14 14 21 14 14 Mean disease rating3 June 29 July 10 July 22 0.00 0.00 0.00 1.00 0.25 0.25 0.00 0.75 0.75 0.50 0.00 0.00 0.00 0.00 0.00 28 0.25 0.50 1.50 14 14 0.25 0.00 0.00 7 14 14 14 0.50 0.75 0.50 0.25 1.00 1.0 0.00 0.00 3.00 0.50 0.50 0.00 14 14 14 14 14 14 14 14 14 0.00 0.00 0.75 0.00 0.75 0.25 1.50 0.50 0.25 1.02 1.50 0.50 0.50 0.00 0.25 0.00 1.50 1.00 0.25 0.98 1.00 0.00 0.50 0.00 3.25 0.00 0.50 1.50 0.25 0.93 aRating Scale: 0 = no disease; 1 = 1-5%; 2 = 5-10%; 3 = 10-25%; 4 = 25-50%; and 5 = >50% of plot diseased. bP = 0.05. n = 4 replications. 75 Fertilizer Trials Creeping Bentgrass Establishment and Management on Sand Greens Michael B. Faust and Nick E. Christians INTRODUCTION This creeping bentgrass establishment trial was initiated on 1 Sept. 1996 at the Iowa State University Horticulture Research Station. Data from the 1998 growing season are discussed in this report. The study was conducted to observe the development o f creeping bentgrass from seed grown in a sand-based golf course green and to study the effects o f liquid and granular fertilizer applications on the quality of mature bentgrass. The objectives of the study were i) to compare the effects of five different combinations of organic-based fertilizer products and a control on the establishment of creeping bentgrass (Agrostis palustris Huds. cv. Crenshaw), and ii) to compare the effects o f two different application frequency schedules on the quality of mature creeping bentgrass. MATERIALS AND METHODS The research was conducted on a 100% sand-based golf green. The rooting material contained 10% calcium carbonate (CaC03) and had a pH o f 8.2. Physical analysis of the sand particles showed the rooting medium to be within the standards set by the United States Golf Association (USGA) for golf course green construction. The research, with a 900 ft2 area, was conducted as a split-plot design with six treatments as main plots and two application frequency schedules as subplots. The study included three replications. Each experimental plot (36 total) had an area of 25 ft2. The main plots o f the research consisted of five liquid fertilizer products and a granular fertilizer material (Tables 1, 2, and 3). Four of the liquid treatments, excluding the control, were general use organic soil conditioners designed to stimulate microbial activity and to provide overall improved soil fertility. The organic liquid treatments were mixed in solution with NH4 NO3 and KN0 3 to supply adequate nitrogen and potassium requirements of the plant. The control treatment contained only the inorganic nitrogen (NH4 NO3 ) and potassium (KN03) sources. All of the liquid treatments contained the same rate of nitrogen and potassium, and they were applied to the turf using a C 0 2 tank and hand-held spray boom. Granular treatments were applied with a hand-held shaker to match the N and K rates of the liquid products. The first application to experimental plots in the spring of 1998 occurred on 21 May. The application frequency schedule established in 1997, where half of the experimental plots received one application every two weeks and the other half of the plots received two applications per week, was continued in 1998. Plots treated once every two weeks received 0.25 lb N and 0.125 lb K/1000 ft2 /application. Plots treated two times per week received 0.0625 lb N and 0.0313 lb K/1000 ft2 /application. All plots received 0.5 lb N and 0.25 lb K/1000 ft2/month irregardless of application frequency schedule or treatment differences. Clipping tissue samples were collected three times during the 1998 season. Individual collection dates were 17 and 31 August, and 14 September. Clippings were taken 3 to 4 days following the fourth and eighth 0.0625 lb N/1000 ft2 treatment application when all plots had received identical N and K rates. The clippings were dried at 6 8 °C for 48 h, dry-ashed, diluted with acid, and analyzed for nutrient content by inductively coupled argon plasma spectrometry (ICAP). Plant tissue nitrogen content was determined using the total Kjeldahl nitrogen procedure (TKN). One root sample was taken during the 1998 growing season on 1 October. Five one-inch diameter cores were removed from random locations on each plot at a depth of 15 cm. The roots were washed from the sand media using a screening technique. The extracted root material was dried at 6 8 °C for 48 h. An oven-dry root mass was taken and the samples were placed into a muffle furnace for 12 h at a temperature of 500 °C. A second root weight was taken following the ashing procedure. The actual dry root mass of plants grown on each experimental plot was determined by subtracting the dry-ashed root mass from the oven-dry root mass. Visual quality data, rating density and color of each plot was taken four times in 1998. The data was collected 17 and 31 August, and 8 and 14 September. Quality was rated on a 1 to 9 scale; where 1 = poor quality and 9 = highest quality. RESULTS AND DISCUSSION Results from clipping analysis are shown in Tables 1 and 2. The tables have been divided into macronutrient (Table 1) and micronutrient (Table 2) concentrations of turfgrass tissue. 76 Fertilizer Trials Differences in plant shoot tissue concentration due to treatment effects were shown for the elements nitrogen (N), phosphorus (P), magnesium (Mg), copper (Cu), and molybdenum (Mo) (Tables 1 and 2). Compared to the five liquid treatments, the granular treatment provided the highest tissue N, P, and Mg concentrations. Plants grown in control plots had the lowest tissue N levels. Phosphorus shoot tissue concentration of plants grown using the granular treatment was on average 36% higher than plants supplied with the liquid treatments. Tissue P concentrations were probably higher because the granular product contained 3% P2 0 5 (12-3-9 formulation). Phosphorus was not supplied to plots receiving the liquid treatments throughout the 1998 growing season. Plants fertilized by the liquid treatments had significantly more Cu in the shoot tissue as compared to those plants grown using the granular material. Plants grown using the control and granular treatments had significantly lower Mo tissue levels as compared to the other liquid treatments. The high organic matter content of the granular product may have complexed Cu and Mo reducing the ability of the plant to absorb these elements. Application frequency of treatments (2 vs. 8 applications/month) caused differences in shoot tissue concentration for the elements: nitrogen (N), copper (Cu), and molybdenum (Mo) (Tables 1 and 2). Nitrogen shoot tissue concentration was 3% higher for plants grown in plots receiving 8 applications/month compared to those plants receiving 2 applications/month. Shoot tissue concentrations were 6 % and 8 % higher for Cu and Mo, respectively, in plots receiving 8 applications/month compared to the plots which received 2 applications/month. However, plants grown in plots receiving 2 applications/month had an average 8 % higher shoot tissue Mn concentration compared to plants grown in plots that received 8 applications/month. Mean visual quality data taken throughout 1998 showed differences among fertilizer treatments (Table 3). The granular product (treatment 6 ) had significantly higher visual quality ratings compared to the liquid treatments. The better visual quality could be explained by the shoot tissue nutrient concentration data which showed the highest N levels in plants treated with the granular feather meal product. Plots receiving 8 applications per month had a higher visual quality rating than plots receiving 2 applications/month. No differences were shown between treatments or application frequency schedules for root development of grass plants (Table 3). These findings are similar to rooting data taken in 1997. T a b ic 1. Mean macronutrient tissue concentration and analysis of variance.____________ M a c r o n u tr ie n ts * Nitrogen (N) Phosphorus 22% humic acid 3.39 0.26 6-0-0 w/ organic acids 3.45 0.26 T r e a tm e n t (P) Calcium (Ca) Magnesium (Mg) Sulfur (S) 1.83 0.74 0.28 0.25 1.87 0.72 0.27 0.25 Potassium (K) /O U1 Uiy llaallC 15% humic acid 3.44 0.26 1.87 0.70 0.26 0.25 5-3-2 w/ molasses 3.36 0.26 1.89 0.70 0.26 0.26 Control 3.23 0.25 1.85 0.74 0.27 0.25 12-3-9 ground feather meal 3.47 0.40 1.87 0.77 0.30 0.25 0.14 0.02 NS NS 0.02 NS 3.34 0.28 1.85 0.74 0.28 0.25 8 apps/month 3.43 0.28 1.87 0.71 0.27 0.25 L S D io.o5iy 0.08 NS NS NS NS NS 0.0146 0.0001 0.5884 0.2256 0.0038 0.2515 0.3652 0.1965 0.2247 0.1076 L S D io.o5iy A p p lic a tio n F r e q u e n c y 2 apps/month A nova* P rob > F Treatment Application frequency 0.0303 0.1851 0.4941 0.7280 0.3067 0.2056 Trt* Application frequency 0.0053 0.6029 zData shown are the mean of three tissue collection dates during the 1998 growing season. Individual collection dates were 17 and 31 August, and 14 September. yMean separation within columns by Fisher’s least significant difference test. Significant differences occur at the P < 0.05 level 77 Fertilizer Trials T a b le 2 . Mean micronutrient tissue concentration and analysis of variance. M ic |r |o n u tr ie n tsz Boron (B) Copper (Cu) Iron (Fe) Manganese (Mn) - mg Kg Molybdenum (Mo) Sodium (Na) Zinc (Zn) 22% humic acid 5.37 8.80 213.17 158.17 2.93 64.08 61.49 6-0-0 w/ organic acids 5.52 9.33 205.44 148.37 2.78 60.56 61.47 60.43 62.70 64.21 T r e a tm e n t 15% humic acid 5.43 8.82 170.28 158.77 2.92 5-3-2 wl molasses 5.44 8.89 171.33 163.56 2.86 59.66 Control 5.57 8.99 214.61 166.41 2.61 62.11 62.08 12-3-9 ground feather meal 5.38 6.98 230.89 158.95 2.39 70.07 64.16 NS 0.87 NS NS 0.31 NS NS 2 apps/month 5.47 8.37 206.06 165.32 2.64 62.00 62.94 8 apps/month 5.44 8.90 195.85 152.76 2.86 63.63 62.43 LSD(oo5iy NS 0.50 NS 11.18 0.18 NS NS Treatment 0.8838 0.0002 0.2186 0.5175 0.0082 0.0751 0.7501 Application frequency 0.7977 0.0387 0.5379 0.0294 0.0198 0.4422 0.7220 LSD/0.05/ A p p lic a tio n F r e q u e n c y A nova* P rob > F 0.1156 0.6871 0.8790 0.9758 0.8349 0.3775 0.8895 Trt* Application frequency zData shown are the mean of three tissue collection dates during the 1998 growing season. Individual collection dates were 17 and 31 August, and 14 September. yMean separation within columns by Fisher’s least significant difference test. xSignificant differences occur at the P < 0.05 level. T a b le 3. Visual quality, rooting data, and analysis of variance.__________________________________ __________________ T r e a tm e n t Aug 17 Aug 31 Visual Quality2 Sept 8 Sept 14 Mean Root Mass (g) October 1 22% humic acid 6.5 6.8 6.7 7.5 6.9 0.56 6-0-0 w/ organic acids 7.3 6.5 6.7 6 .8 6 .8 0.56 15% humic acid 6.7 6.8 6.5 7.0 6 .8 0.52 5-3-2 w/ molasses 6.7 7.2 7.2 6 .8 7.1 0.58 Control 7.0 6.5 6.7 7.5 6 .8 0.53 7.7 0.63 7.5 7.7 7.7 7.8 NS 0.8 0.6 0.5 0.4 NS 2 apps/month 6.7 6.6 6.5 7.0 6.7 0.56 8 apps/month 7.2 7.3 7.3 7.5 7.3 0.57 NS 0.1325 12-3-9 ground feather meal LSD/oo5\y ________ A p p lic a tio n F r e q u e n c y 0.4 0.4 0.3 0.3 0.3 Treatment 0.0955 0.0425 0.0031 0.0029 0.0023 Application frequency 0.0213 0.0031 0.0001 0.0031 0.0001 0.7084 0.1224 0.3320 0.4362 0.0302 0.2090 Trt*Application frequency zVisual quality (Color and Density) was rated on a 9 to 1 scale: l=poor quality; 9=highest quality. yMean separation within columns by Fisher’s least significant difference test. Significance occurs at the P<0.05 level. 0.9255 LSD(o.o5iy A novax P rob>F 78 Environmental Research Corn Gluten Hydrolysate for Weed Control Melissa C. McDade and Nick E. Christians Com gluten hydrolysate (CGH) is an effective natural preemergent control in growth chamber and greenhouse environments. Its performance in the field may be improved by using a carrier. Two carriers for CGH are being investigated in this study, humic acid (RL 37, Liquid Seaweed Foliar from International Ag Labs, Inc., Fairmont, MN) and a soybean oil (SprayTech Oil from Agro-K Corporation, Minneapolis, MN). CGH at rates o f 0, 10, 20 and 40 lb/1000 ft2, humic acid at rates o f 0, 1 ,2 and 4 gal/acre and oil at rates o f 0, 0.5, 1 and 2 pts/acre were used, with each rate o f CGH being applied with each rate o f humic acid and oil for a total o f 32 treatments. The treatments were randomized in each of three replications. Each individual plot measured 5’x 5’. This study took place at the ISU Horticulture Research Station north o f Ames, Iowa in an area with a mature stand o f Kentucky bluegrass. Treatments were sprayed onto the plots on 9 May. The study area was irrigated as needed to provide a good growing condition for the turf. Weed control was determined by collecting data on percent cover o f crabgrass during the season. Visual quality was also assessed during the season using a 9 to 1 scale: 9 = best quality, 6 = lowest acceptable quality, 1 = poorest quality. Analysis o f data used the Statistical Analysis System version 6.12 (SAS Institute, 1989-1996). There were 72% fewer crabgrass plants at the greatest rate o f CGH (200 gm"2) compared to the control (Table 1). Increasing CGH rates had a positive effect on turfgrass visual quality (Table 1). Humic acid and soybean oil had no effect on the number o f crabgrass plants or on turfgrass quality. CGH applied at a rate o f 200 g m -2 provided 72% reduction o f crabgrass in this study, similar to a 6 6 % reduction found in the first year o f another CGH field study at the 200 g m"2 rate (Bingaman and Christians, 1996). This reduction is also similar to that o f com gluten meal, which provided 8 6 % control when applied to turf at the 2 0 0 g m ' 2 rate (Bingaman et al., 1998). Rates o f 50 and 100 g CGH per m 2 did not control crabgrass in this study and varied in effectiveness in previous studies by Bingaman and Christians (1996, 1997, 1998). Applying 200 g m "2 CGH provides 20 g N per m2, since CGH is 10% N by weight. The CGH has a fertilizing effect, improving the visual quality o f the turfgrass at higher rates. Other studies have reported this fertilizing effect in both CGH (Bingaman and Christians, 1996, 1997, 1998) and com gluten meal (Bingaman et al., 1998; Christians, 1993). If soybean oil or humic acid had a positive effect on the herbicidal activity o f the CGH, we would have seen similar crabgrass counts or quality at different rates o f CGH because o f the addition o f soybean oil or humic acid. Only the rate o f CGH had an effect on crabgrass counts and quality, so the soybean oil and humic acid treatments had no effect, neither as interactions nor as single effects. Table 1. Mean number of crabgrass (Digitaria spp.) plants at the end of the 1998 growing season (16 weeks). Mean turfgrass quality from the 1998 season, rated visually: 9 = highest quality, 6 = acceptable quality, 1 = lowest ________ quality. Each rate of com gluten hydrolysate was applied to 10 plots in each of three replications._________ Turfgrass Number of crabgrass plants Com gluten hydrolysate visual quality (per 2.25 n r plot) (gm '2) 6.0 29 0 17 6.5 50 7.0 26 100 8.0 8 200 0.2 13 L S D o .05 REFERENCES Bingaman, B.R. and N.E. Christians. 1998. 1995 com gluten hydrolysate weed control study - year 3. Iowa Turfgrass Res. Rpt., Iowa State Univ. Ext. p. 103-104. Bingaman, B.R. and N.E. Christians. 1997. 1995 com gluten hydrolysate weed control study - year 2. Iowa Turfgrass Res. Rpt., Iowa State Univ. Ext. p. 75-76. Bingaman, B.R. and N.E. Christians. 1996. 1995 corn gluten hydrolysate weed control study. Iowa Turfgrass Res. Rpt., Iowa State Univ. Ext. p. 87-88. Bingaman, B.R., N.E. Christians, and M.B. Faust. 1998. 1991 com gluten meal crabgrass control study - year 7. Iowa Turfgrass Res. Rpt., Iowa State Univ. Ext. p. 95-98. Christians, N.E. 1993. The use of corn gluten meal as a natural preemergence weed control in turf, p. 284-290. In: Carrow R.N., N.E. Christians, R.C. Shearman (eds.). Inti. Turfgrass Soc. Res. J. 7. Intertec Publishing Corp., Overland Park, KS. 79 Environmental Research 1991 Corn Gluten Meal Crabgrass Control Study - Year 8 Barbara R. Bingaman, Melissa C. McDade, and Nick E. Christians A study screening com gluten meal (CGM) for efficacy as a natural product herbicide and fertilizer in turf has been continued on the same plot for since 1991. It is being conducted at the Iowa State University Research Station north o f Ames, IA in an area o f ’Parade' Kentucky bluegrass. The soil in this experimental area is a Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll) with an organic matter content o f 3.6% a pH o f 7.1, 4.5 ppm P, and 101 ppm K. Individual experimental plots are 5 x 5 ft and there are 5 treatments with 3 replications. The experimental design is a randomized complete block. Com gluten meal is applied each year to the same plots at 0, 20, 40, 60, 80, 100, and 120 lbs/1000 ft2 (Table 1). Because com gluten meal is 10% N, these rates are equivalent to 0, 2 ,4 , 6, 8, and 10 lb N/1000 ft2. In 1998, the CGM was applied in a single early spring preemergence application on April 21 using ’shaker dispensers'. The materials were watered-in with the irrigation system. Supplemental irrigation was used to provide adequate moisture to maintain the grass in good growing condition. The plot was monitored throughout the season for turf quality. Visual turf quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst turf quality. Quality data were taken on April 22, May 13, May 18, May 27, June 10, June 16, July 9, July 15, July 29, August 18, and August 28 (Tables 1 and 2). Weed control was measured by either counting the number of plants or estimating the percentage cover per individual plot. Crabgrass in the 1- to 3-leaf stage was found in the untreated turf on June 23. The crabgrass plants were large enough by July 15 to count the number per plot. Additional crabgrass count data were taken on July 29, August 18, and August 28 (Table 3). Crabgrass counts were converted to express percentage reductions in numbers as compared to the untreated controls (Table 4). Dandelion and clover populations also were surveyed. The number o f dandelions per plot was counted on July 15, July 29, August 18, and August 28 (Table 5). On these same dates, percentage clover cover data were taken. Clover cover was determined by estimating the area per plot covered by clover (Table 7). Dandelion count and percentage clover cover data were modified to express percentage reductions as compared to the untreated controls (Tables 6 and 8). Data were analyzed with the Statistical Analysis System (SAS , Version 6.12) and the Analysis o f Variance (ANOVA) procedure. Fisher’s Least Significant Difference (LSD) means comparison tests were used to assess CGM effects on bluegrass quality and weed control. Spring greenup was obvious by April 22. Bluegrass treated with CGM had better quality than untreated turf from May 13 through June 16 (Table 1 and 2). After June 16, the season was quite hot and dry and the treated and untreated bluegrass had similar quality. Crabgrass populations were low in the untreated controls because the dandelion and clover infestations were large and well established creating intense competition for the emerging crabgrass. Treatment with CGM at all levels except 20 lb/1000 ft2 resulted in much lower crabgrass populations (Table 3). Reductions in crabgrass populations were > 91% at 40 and 60 lb/1000 ft2 (Table 4). There were more crabgrass plants in turf treated with CGM at 20 lb/1000 ft2 than in untreated turf (Table 3). In 1997, there also were more crabgrass plants in turf treated with CGM at 20 lb/1000 ft2 as compared with the untreated control (Table 9). Reductions in crabgrass counts were similar to 1997 at all CGM levels except 40 lb/1000 ft2. Much better crabgrass control was achieved at 40 lb in 1998 as compared with 1997. Com gluten meal at all levels except 20 lb/1000 ft2 significantly reduced dandelion counts as compared with the untreated control (Table 5). Mean reductions > 72% were achieved with CGM at 40 lb and higher (Table 6). Dandelion control in 1998 was similar to control in 1997 and previous years except at the 20 lb/1000 ft2 level (Table 11). On July 29 and August 18, there was significantly less clover cover in turf treated with CGM as compared with the untreated controls (Table 7). On August 28, all CGM levels except 20 lb/1000 ft2 significantly reduced clover cover. Mean reductions in clover cover were > 84% as compared with the untreated controls in turf treated with CGM at all levels except 20 lb (Table 8). Clover control in 1998 was similar to that in previous years except at 20 lb/1000 ft2. At this rate, the level o f clover control was much lower in 1998 as compared to 1994 through 1997 (Table 10). 80 Environmental Research Table 1 Visual quality1 o f Kentucky bluegrass treated in the 1991 Com Gluten Meal Weed Control Study. 1 2 3 4 5 6 7 Material Untreated control Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal lbs CGM/ 1000 ft2 0 20 40 60 80 100 120 lbs N / 1000 ft2 0 2 April May 13 5.0 5.7 7.0 8.7 7.7 8.3 9.0 1.4 22 6.7 6.3 7.3 8.3 8.0 8.3 9.0 1.1 4 6 8 10 12 L S D o os May 18 5.3 6.3 7.7 8.7 8.0 8.3 8.3 0.8 May 27 5.0 6.3 8.0 8.0 8.3 8.0 7.7 1 .4 June 10 5.7 6.3 7.7 8.7 8.7 9.0 9.0 0.6 June 16 5.0 6.3 7.3 8.3 9.0 8.7 9.0 0.6 'Visual quality was assessed using a 9 to 1 scale with 9 = best, 6 = least acceptable, and 1 = worst turf quality. NS = means are not significantly different at the 0.05 level. Table 2. Visual quality1 o f Kentucky bluegrass treated in the 1991 Com Gluten Meal Weed Control Study. 1 2 3 4 5 6 7 Material Untreated control Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal lbs CGM/ 1000 ft2 0 20 40 60 80 100 120 July L S D 005 9 6.0 6.0 6.0 6.0 6.0 6.0 6.0 - July 15 6.0 6.0 6.0 6.0 6.0 6.0 6.0 July 29 6.0 6.0 6.0 6.0 6.0 6.0 6.0 August 5 6.0 6.0 6.0 6.0 6.0 6.0 6.0 August 18 6.0 6.0 6.0 6.0 6.0 6.0 6.0 August 28 6.0 6.0 6.0 6.0 6.0 6.0 6.0 — — — — — Mean 5.4 6.2 7.5 8.4 8.3 8.7 8.7 0.6 V isual quality was assessed using a 9 to 1 scale with 9 = best, 6 = least acceptable, and 1 = worst turf quality. —= these data are not appropriate for means comparisons tests. Table 3. Crabgrass count1 in Kentucky bluegrass treated in the 1991 Com Gluten Meal Weed Control Study. 1 2 3 4 5 6 7 Material Untreated Control Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal lbs CGM/ 1000 ft2 0 20 40 60 80 100 120 L SD oos July 15 6.3 5.3 3.7 0.3 8.7 4.0 1.7 NS July 29 22.7 38.3 1.0 1.0 9.0 4.3 7.0 NS August 18 29.7 41.3 0.7 3.7 12.0 3.0 6.0 NS August 28 21.3 36.7 1.7 1.7 5.7 2.0 4.7 NS Mean counts 20.0 30.4 1.8 1.7 8.8 3.3 4.8 NS 1These values represent the number of crabgrass plants per plot. NS = means are not significantly different at the 0.05 level. Table 4. Percentage crabgrass count reductions1 in Kentucky bluegrass treated in the 1991 Com Gluten Meal Weed Control Study. Material lbs CGM/ 1000 ft2 July 15 July 29 August 18 August 28 Mean reduction % 0.0 0.0 0.0 0.0 0.0 15.7 0.0 0.0 0.0 0.0 42.1 95.6 97.8 92.2 91.3 94.7 95.6 87.6 92.2 91.7 0.0 59.6 73.4 60.3 55.8 89.9 36.8 80.9 90.6 83.3 73.7 69.1 79.8 78.1 75.8 NS NS NS NS NS L S D o 05 “T77 rThese values represent percentage reduction in crabgrass plants per plot as compared with the untreated controls. NS = means are not significantly different at the 0.05 level. 1 2 3 4 5 6 7 Untreated Control Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal 0 20 40 60 80 100 120 81 Environmental Research T able 5. Dandelion count1 in Kentucky bluegrass treated in the 1991 Com Gluten Meal W eed Control Study. lbs CGM/ July July August August Mean Material 1000 fit2 15 29 18 28 counts 1. Untreated control 47.7 0 25.0 25.7 48.7 36.8 2. 40.7 49.7 Com gluten meal 20 25.0 24.7 35.0 40 15.3 12.0 Com gluten meal 3. 9.0 4.7 10.3 4. Com gluten meal 60 3.0 8.3 8.7 3.7 5.9 4.7 5. Com gluten meal 80 3.7 1.3 3.3 3.3 5.0 6. Com gluten meal 100 4.0 5.3 1.3 3.9 1.3 2.7 7. Com gluten meal 120 0.3 0.7 1.3 15.2 29.6 13.6 11.0 13.7 L SD oos ‘These counts represent the number o f dandelions per plot. Table 6. Percentage dandelion count reductions1 in Kentucky bluegrass treated in the 1991 Com Gluten Meal Weed Control Study. July July August August lbs CGM/ Mean 18 28 Material 1000 ft2 15 29 reduction 0.0 0.0 0.0 3.9 64.0 81.8 85.3 88.3 85.3 94.8 84.0 94.8 98.7 97.4 54.5 43.0 L SD oos 'These values represent the percentage reduction in dandelions per plot as 1. 2. 3. 4. 5. 6. 7. Untreated control Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal 0 20 40 60 80 100 120 % 0.0 0.0 0.0 14.7 0.0 4.8 67.8 75.3 72.1 82.5 82.2 83.9 93.0 90.4 91.2 89.5 89.0 89.3 97.2 94.5 96.6 31.9 60.8 37.3 compared with the untreated controls. Table 7. Percentage clover cover1 in Kentucky bluegrass treated in the 1991 Com Gluten Meal Weed Control Study. August August Mean July July lbs CGM/ 18 28 cover 29 Material 1000 ft2 15 43.3 53.3 43.7 1. Untreated control 0 34.7 43.3 30.0 41.7 32.1 20 35.0 21.7 2. Com gluten meal 3.3 5.3 6.9 40 10.7 8.3 3. Com gluten meal 6.7 7.0 5.3 6.4 60 4. Com gluten meal 6.7 3.7 3.7 3.2 5. Com gluten meal 80 3.3 2.0 2.0 5.3 4.5 100 6. Com gluten meal 7.0 3.7 3.7 2.3 6.7 5.3 4.5 120 7. Com gluten meal 14.3 13.1 9.3 NS 15.7 L SD oos ‘Percentage clover cover represents the area per plot covered by clover. Table 8. Percentage clover cover reductions1 in Kentucky bluegrass treated in the 1991 Com Gluten Meal Weed Control Study. August August Mean lbs CGM/ July July 28 1000 ft2 15 29 18 reduction Material % 0.0 0.0 0.0 0.0 30.8 21.9 50.0 26.5 92.3 90.0 80.8 84.2 4. 87.7 87.5 83.8 85.3 5. 95.4 91.5 93.1 92.7 95.4 6. 90.0 91.5 89.7 91.5 95.6 7. 87.7 89.7 30.2 26.9 NS 36.2 21.2 L SD oos ~T=7 ^ h ese values represent the percentage reduction in clover cover per plot as compared with the untreated controls. 1. 2. 3. Untreated control Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal 0 20 40 60 80 100 120 0.0 0.0 69.3 80.8 90.4 79.8 70.8 82 Environmental Research Table 9. Comparisons o f the mean percentage crabgrass count reductions1 in Kentucky bluegrass treated in the _________ 1991 Com Gluten Meal Weed Control Study for 1991 through 1998.______________________________ lbs Material CGM/ 1991 1992 1993 1994 1995 1996 1997 1998 1000 fit2 % 1 2 3 4 5 6 7 Untreated control 0 0 0 0 20 58 85 Com gluten meal 91 40 86 Com gluten meal 98 98 60 97 98 Com gluten meal 93 80 87 Com gluten meal 93 93 Com gluten meal 100 79 94 95 120 Com gluten meal 97 100 100 44 26 31 l s d 005 'These values represent the percentage reduction in crabgrass plants controls. NS = means are not significantly different at the 0.05 level. 0 70 97 98 87 86 98 39 0 36 88 93 75 75 84 40 0 15 97 85 69 87 97 60 0 0 79 82 54 79 82 NS 0 0 91 92 56 83 76 NS per plot as compared with the untreated Table 10. Comparisons o f the mean percentage clover cover reductions in Kentucky bluegrass treated in the 1991 Com Gluten Meal Weed Control Study for 1994 through 1998. lbs CGM/ 1994 1000 ft2 1995 1996 Material 1997 1998 % 0 71 82 93 90 92 93 29 0 0 0 0 81 63 27 56 90 84 64 87 95 98 85 93 100 95 93 76 76 94 84 90 93 90 90 93 26 21 48 NS L S D 00 5 ‘Percentage clover cover represent the area per plot covered by clover. These values represent the percentage reduction o f clover cover per plot as compared with the untreated controls. 1 2 3 4 5 6 7 Untreated control Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal 0 20 40 60 80 100 120 Table 11. Comparisons o f the mean percentage dandelion count reductions1in Kentucky bluegrass treated in the 1991 Com Gluten Meal Weed Control Study for 1994 through 1998. lbs CGM/ 1994 1996 Material 1000 ft2 1995 1997 1998 0/n /O 0 0 0 0 0 71 33 24 5 49 100 75 76 72 77 84 83 100 89 79 98 95 93 91 96 88 100 98 96 89 100 100 97 97 100 50 60 61 37 65 L S D 00 5 'Dandelion counts represent the number o f dandelions per plot. 2These values represent the percentage reduction o f dandelion count per plot as compared with the untreated controls. 1 2 3 4 5 6 7 Untreated control Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal Com gluten meal 0 20 40 60 80 100 120 83 Environmental Research 1995 Corn Gluten Meal Rate Weed Control Study - Year 4 Barbara R. Bingaman, Melissa C. McDade, and Nick E. Christians Com gluten meal (CGM) is being screened for efficacy as a natural product herbicide in turf in this long-term study begun in 1995. It is being conducted at the Iowa State University Horticulture Research Station north o f Ames, IA in established ’Ram 1' Kentucky bluegrass. The soil is a Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll) with an organic matter content o f 3.8%, a pH o f 7.1, 4 ppm P, and 96.5 ppm K. The initial broadleaf weed population exceeded 50% cover on most o f the test area. Individual experimental plots are 10 x 10 ft with 4 treatments and 3 replications. The experimental design is a randomized complete block. Each year com gluten meal is applied at a yearly rate o f 40 lb. CGM/1000 ft2 (equivalent to 4 lb. N/1000 ft2) using 4 different regimes of single and split applications (Table 1). Four applications o f 10 lb./lOOO ft2, split applications o f 20 lb./lOOO f t 2, an initial application o f 30 lb. plus a sequential o f 10 lb./lOOO ft2, and a single application o f 40 lb./lOOO ft2 are included with an untreated control. Initial applications for 1998 were made on April 21 before crabgrass germination. The second application o f treatment 2 was made on June 3, the third on July 14, and the final on August 26. Sequential applications o f treatments 3 and 4 were made on July 14. The experimental plot was checked for phytotoxicity after each treatment. The study was monitored for visual quality throughout the season. Visual quality was measured using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality (Tables 1 and 2). Visual quality data were taken on April 22, May 13, May 18, May 27, June 10, June 18, June 30, July 9, July 15, July 24, July 29, August 11, August 18, and August 26. Populations o f annual grass and perennial broadleaf weed species were surveyed. Crabgrass populations were assessed by counting the number o f plants per individual plot on July 9, July 24, July 29, August 11, August 18, and August 26 (Table 3). Clover infestations were estimated by determining the percentage o f area in each individual plot covered by clover. These data were taken on July 29, August 11, August 18, and August 26 (Table 5). Dandelion populations were measured by counting the number o f plants per plot on July 29, August 11, August 18, and August 26 (Table 7). Levels o f crabgrass, clover, and dandelion control were expressed by converting the population data to reductions as compared with the untreated control (Table 4, 6, and 8). Data were analyzed with the Statistical Analysis System (SAS, Version 6.12) and the Analysis o f Variance (ANOVA) procedure. Means comparisons were made with Fisher’s Least Significant Difference test (LSD). There were no phytotoxic symptoms detected on the treated bluegrass. Visual turf quality was significantly better in bluegrass treated with CGM than in the untreated control on May 13, May 27, June 10, June 18, June 30, July 9, August 11, August 18, and August 26 (Table 1 and 2). Mean visual quality for the entire season was better for bluegrass treated with CGM than the untreated grass. Crabgrass populations were lower in turf treated with CGM than in untreated turf but the counts were not statistically different (Table 3). Crabgrass numbers were low in the untreated controls. Broadleaf weed species were well established when the crabgrass was emerging especially in the untreated controls and the competition from the broadleaves and the mature turf probably prevented the establishment o f large crabgrass populations within the untreated plots. Reductions in crabgrass populations were not significant for any o f the CGM treatments on any o f the collection dates but there were large numerical reductions achieved in treated bluegrass as compared with untreated turf (Table 4). Bluegrass treated with split applications o f 20 lb. CGM reduced crabgrass counts by > 89% from July 9 through August 11. In 1998, crabgrass control was much better overall than in 1995, 1996, and 1997 (Table 9). In contrast to 1996 and 1997, there was more crabgrass in the untreated controls than in treated grass in 1998. Split applications o f 20 lb. CGM provided 86% crabgrass reductions in 1998 as compared to 45, 33, and 50% in 1995, 1996, and 1997, respectively. There was significantly less clover in CGM treated turf than in the untreated controls on all data collection dates except August 18 (Table 5). All CGM rates significantly decreased clover populations and clover cover was lowest in turf treated with a single application o f 40 lb. CGM. The mean clover cover in untreated turf was 40.4% as 84 Environmental Research compared to 10.7, 11.4, 14.7, and 3.0% cover in turf receiving treatments 2, 3 ,4 , and 5, respectively. The single application o f 40 lb. CGM provided > 89% reductions in clover cover throughout the season as compared to the untreated control (Table 6). Clover control was better in 1998 than 1996 and 1997 in turf treated with CGM four times at 10 lb. (Table 10). Clover control in 1998 with split applications o f 20 lb. was slightly less than in 1997 and similar to 1996. Control provided by CGM at 30 lb. followed by 10 lb. was much lower in 1998 as compared with 1996 and 1997. A single application at 40 lb. provided similar clover cover reductions in 1998 as in 1996 and slightly lower than 1997. Dandelion cover was significantly less in all CGM treated turf as compared to the untreated control on all data collection days except August 26 (Table 7). Percentage reductions in dandelion cover were similar for all CGM treatments (Table 8). In 1998, dandelion control was higher than in 1996 and 1997 when CGM was applied four times at 10 lb. and in a single application at 40 lb. (Table 11). Dandelion reductions were similar in 1996, 1997, and 1998 for CGM at 20 lb. split applications. Percentage reductions were much higher in 1998 as compared with 1996 and 1997 for CGM applied at 30 lb. followed by 10 lb. Table 1. Visual quality1 o f Kentucky bluegrass treated with com gluten meal in the 1995 Com Gluten Meal Rate Material 1. 2. Untreated control Com gluten meal 3. 4. 5. Com gluten meal Com gluten meal Com gluten meal Rate (lb. product /1000 ft2) April 22 May 13 May 18 May 27 June 10 June 18 June 30 0 10 fb lO.fb 10 fb 10 20 fb 20 30 fb 10 40 6.3 8.7 6.0 8.7 6.3 7.0 5.3 7.0 5.0 6.7 6.0 7.3 6.0 7.0 7.7 8.0 7.0 8.7 9.0 8.7 8.7 7.7 8.7 7.7 8.7 8.3 7.0 8.3 9.0 7.0 8.3 8.7 7.3 8.3 9.0 1.4 0.8 0.6 0.9 0.7 NS 0.8 L SD oos ‘Visual quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst turf quality. All treatments were at an annual rate of 4 lb.s N/1000 ft2. Initial applications were made on April 21. Second application of trt 2 was made on June 3, the third on July 14, and the final on August 26. Sequential applications of trt 3 & 4 were made on July 14. NS = means are not significantly different at the 0.05 level Table 2. Visual quality1 o f Kentucky bluegrass treated with com gluten meal in the 1995 Com Gluten Meal Rate Weed Control Study (July 9 - August 26). Material 1. 2. Untreated control Com gluten meal 3. 4. 5. Com gluten meal Com gluten meal Com gluten meal Rate (lb. product /1000 ft2) July 9 July 15 July 24 July 29 Aug 11 Aug 18 Aug 26 Mean 0 10 fb 10 fb 10 fb 10 20 fb 20 30 fb 10 40 6.0 7.0 6.0 8.0 6.0 8.0 6.0 8.0 6.0 7.3 6.7 8.3 6.0 8.0 6.0 7.6 7.3 8.0 8.3 7.3 7.7 8.3 7.3 7.7 8.3 7.7 8.3 7.0 7.0 7.3 7.0 8.3 8.3 8.3 9.0 8.3 7.3 7.7 8.1 8.1 0.6 0.5 0.7 1.2 0.7 1.1 1.6 NS L S D o os _________ ‘Visual quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst turf quality. NS = means are not significantly different at the 0.05 level 85 Environmental Research Table 3. Crabgrass counts per plot1 in Kentucky bluegrass treated with com gluten meal (CGM ) in the 1995 Com Gluten Meal Rate Weed Control Study. Material 1. 2. Untreated control Com gluten meal 3. 4. 5. Com gluten meal Com gluten meal Com gluten meal Rate (lb. product /1000 ft2) July 9 July 24 July 29 August 11 August 18 August 26 Mean NA 10 fb 10 fb 10 fb 10 20 fb 20 30 fb 10 40 8.7 3.3 13.3 9.0 16.7 6.7 18.3 11.7 23.3 11.7 31.7 23.3 18.7 10.9 0.3 1.0 5.0 0.7 2.3 8.3 1.7 2.0 6.7 2.0 .7.0 10.0 3.7 5.3 6.7 7.0 7.3 16.7 2.6 4.2 8.9 NS NS L SD oos "ITT 1These values represent the number of crabgrass plants per plot. NS = means are not significantly different at the 0.05 level. NS NS NS NS NS Table 4. Crabgrass count reductions1 in Kentucky bluegrass treated with com gluten meal (CGM) in the 1995 Com Gluten Meal Rate Weed Control Study. Material Rate (lb. product / 1 000 ft2) July 9 July 24 July 29 NA 10 fb 10 fb 10 fb 10 20 fb 20 30 fb 10 40 0.0 61.7 0.0 32.3 0.0 60.1 96.2 88.5 42.5 95.0 82.5 37.3 90.0 88.0 60.1 August 11 August 18 August 26 Mean 0.0 36.2 0.0 50.0 0.0 26.4 0.0 41.5 89.1 61.7 45.4 84.3 77.1 71.4 77.9 76.9 47.4 86.3 77.7 52.5 0/n /o 2. Untreated control Com gluten meal 3. 4. 5. Com gluten meal Com gluten meal Com gluten meal 1. NS NS NS NS NS NS NS L S D o os ‘These values represent the percentage reduction in crabgrass plants per plot as compared with the untreated controls. All treatments were at an annual rate of 4 lb.s N/1000 fr. Initial applications were made on April 21. Second application of trt 2 was made on June 3, the third on July 14, and the final on August 26. Sequential applications of trt 3 & 4 were made on July 14. NS = means are not significantly different at the 0.05 level. Table 5. Percentage clover cover per plot1 in Kentucky bluegrass treated with com gluten meal in the 1995 Com Gluten Meal Rate Weed Control Study. Rate (lb. product/1000 ft2) July 29 August 11 August 18 August 26 Mean NA 10 fb 10 fb 10 fb 10 20 fb 20 30 fb 10 40 40.0 10.7 10.0 11.7 1.7 41.7 8.3 12.0 16.0 1.7 % 31.7 7.0 6.7 14.3 3.3 48.3 16.7 17.0 16.7 5.3 40.4 10.7 11.4 14.7 3.0 20.5 LSD oos “TTT ‘These data represent the area per plot covered by clover. NS = means are not significantly different at the 0.05 level. 16.5 NS 21.4 19.6 Material 1. 2. 3. 4. 5. Untreated control Com gluten meal Com gluten meal Com gluten meal Com gluten meal 86 Environmental Research Table 6. Percentage clover cover reductions1 in Kentucky bluegrass treated with com gluten meal in the 1995 Com Gluten Meal Rate Weed Control Study. Material 1. 2. 3. 4. 5. Untreated control Com gluten meal Com gluten meal Com gluten meal Com gluten meal Rate (lb. product/1000 ft2) July 29 August 11 August 18 August 26 Mean NA 0.0 73.3 75.0 70.8 95.8 0.0 80.0 71.2 61.6 96.0 % 0.0 77.9 79.0 54.8 89.5 0.0 65.5 64.8 65.5 89.0 0.0 73.6 71.7 63.7 92.6 10 fb 10 fb 10 fb 10 20 fb 20 30 fb 10 40 44.4 51.3 39.6 NS 48.5 L S D o os _____i__________ r percentage reductions in clover cover per plot as compared with the untreated control. ‘These values representrrthe NS = means are not significantly different at the 0.05 level. Table 7. Percentage dandelion cover1 in Kentucky bluegrass treated with com gluten meal in the 1995 Com Gluten Meal Rate Weed Control Study. Material Rate ______________ (lb. product/1000 ft2) July August August August Mean 29_________1J_________ 18_________26_____________ ------------------- % ------------------1. 2. 3. 4. 5. Untreated control Com gluten meal Com gluten meal Com gluten meal Com gluten meal NA 10 fb 10 fb 10 fb 10 20 fb 20 30 fb 10 40 L S D 005 25.0 8.3 10.0 6.7 10.0 20.0 5.0 6.7 6.0 6.7 15.0 5.0 6.7 4.3 5.7 28.3 11.7 13.3 10.0 11.7 22.1 7.5 9.2 6.8 8.5 7.1 6.6 4.6 NS 4.7 ‘These figures represent the area per plot covered by dandelions. All treatments were at an annual rate of 4 lb.s N/1000 ft2.Initial applications were made on A was made on June 3, the third on July 14, and the final on August 26. Sequential applications of trt 3 & 4 were made on July 14. NS = means are not significantly different at the 0.05 level. Table 8. Percentage reductions in dandelion cover 1 in Kentucky bluegrass treated with com gluten meal in the Material Rate (lb. product/1000 ft2) August 11 July 29 August 18 August 26 Mean % 1. 2. 3. 4. 5. Untreated control Com gluten meal Com gluten meal Com gluten meal Com gluten meal LSD oos NA 10 fb 10 fb 10 fb 10 20 fb 20 30 fb 10 40 0.0 66.7 60.0 73.3 60.0 0.0 75.0 66.7 70.0 66.7 0.0 66.7 55.6 71.1 62.2 0.0 58.8 52.9 64.7 58.8 0.0 66.1 58.5 69.5 61.5 28.3 33.1 31.0 NS 21.2 1HP1- All treatments were at an annual rate of 4 lb.s N/1000 ft2. Initial applications were made on April 21. Second application of trt 2 was made on June 3, the third on July 14, and the final on August 26. Sequential applications of trt 3 & 4 were made on July 14. 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G cd X ) c/i CD O - § S) 2 cd •2 5 >> o ^ c/5 © i s o o 2 X .3 p Ì ^ 1 S ; 1 £ 1 1 1 11 * 1 30 CO 1 1 1 i—_ 1 1 1 1 40 ü 9 1 1 1 1 1 ' 1 1 1 Ì0 1 1 T3 g CO 1 1 1 1 11 » 1 C e n te r 1 1 0> 9 ^ Ì 1 1 1 1 1 1 1 1 1 50 yard line 111 « CO 1 ; o f fie ld 1 1 1 1 1 1 1 1 1 1 1 ■ 40 d> & 1 T3 § CO 1 1 I 1 1 ■ 20 Soil Modification and Sand-based Systems The Effect of Tarp Color on Turfgrass Growth David D. Minner, Vince Patterozzi, Jeffrey J. Salmond, Jay S. Hudson, and Paul Stevens Many o f our research ideas come from inventive grounds managers in the turfgrass industry. This project is a good example. Vince Patterozzi, Head Grounds Manager Baltimore Ravens, noticed that there was a difference in turf appearance when he used different color rain tarps. After two years o f hearing this comment, Vince put it to me like this, “There is something going on with tarp color, you figure it out”. He arranged with M Putterman & Company Inc. (800-621-0146) to send us several different samples of tarp colors. The study below is what developed from a very astute observation. Thanks Vince. Objective: To determine if tarp color has any effect on turf growth and color. Method: The Putterman Rain Buster Athletic Field tarps used in this study were designed to keep rain or snow o ff o f the field. The tarps are made from 6-ounce polyethylene or 10-ounce vinyl. These tarps are generally placed on the field temporarily and then removed when the rain event ends. Fields are generally covered for only a few hours, but in some cases a tarp may be left on the field for three days or longer. Three different studies have been conducted since the spring o f 1998 on a mature stand o f ‘Midnight’ Kentucky bluegrass. Five-foot by five-foot tarps o f each tarp color were pinned to the ground in a randomized block design with 3 replications. Tarps were placed on April 9 and removed on April 24 in study A. Tarps were placed on 3 November 1998 and removed on 23 March 1999 in study B. Tarps were placed on 23 March and removed 20 April 1999 in study C. Turf color was rated on a scale o f 1-10, 10 = darkest green and 1 = no green color, white/brown, and 6 = lowest acceptable color. Turf growth was rated on a scale o f 1-10, 10 = most growth and 1 = no growth. Results: The data has not been statistically analyzed but the magnitude o f the differences and consistency among replications has been compelling. Studies A and C were similar because they were covered just during spring green-up. Study B was covered during the entire winter and then uncovered just before any green-up or growth occurred. In all studies, tarp color had a dramatic effect on turf color. Yellow, orange, red, and white tarps produced the best turf color with ratings ranging between 6.5 and 10. When compared to the control, turf color was generally enhanced by tarp colors yellow, orange, red, and white. Tarp colors light blue, blue, and purple produced some yellowing that made them inferior to yellow, orange, red, and white. Tarp colors gray, light-green, dark-green, and black produce the most decrease in turf color and they were considered unacceptable. A Covermaster grow tarp and Enkamat were also included in the study even though they are not moisture prevention tarps. Both Covermaster and Enkamat improved turf color compared to the non-covered control plots. It is clear that tarp color has an affect on turf performance. More investigation will be needed to evaluate duration o f tarp cover, season o f tarp cover, heat load under tarps, and potential for diseases, and light penetration. 112 Soil Modification and Sand-based Systems Table 1. Turf color and growth as affected by tarp color. Study A Study B covered for 16 days in April Covered for 140 consecutive days November to March Study C Covered for 29 consecutive days in Turf color 7:5 Turf color 9.5 Turf growth 7.0 Turf color 9.0 Turf growth 8.5 Turf color 8.5 Turf growth 10.0 Red 6.5 8.3 8.7 9.7 9.0 8.7 8.7 Yellow 8.0 8.7 8.3 9.0 7.7 9.0 9.7 White 9.0 8.0 6.7 6.0 5.0 10 5.7 Light blue 5.0 5.0 5.5 6.0 5.0 6.0 6.0 Blue 5.5 6.5 6.5 7.0 7.5 6.0 7.5 Orange Purple 5.0 5.7 6.7 5.0 6.7 4.0 8.0 Gray 2.0 4.0 4.5 4.0 5.5 2.5 7.7 Light green 3.0 3.7 6.7 4.0 6.7 3.7 8.0 Dark green 1.0 2.7 6.0 1.0 7.0 1.0 8.7 Black/white 1.0 3.0 8.0 1.0 6.0 1.0 7.0 1.0 6.0 8.0 1.0 White/black Covermaster 7.0 5.0 5.0 5.0 10.0 3.0 Enkamat 9.7 9.0 5.0 4.0 10.0 5.0 6.0 3.3 1.0 4.0 6.3 1.0 Control no tarp 7.5 113 Soil Modification and Sand-based Systems Calcium Applications to Turf on Sand-based Media Rodney A. St. John and Nick E. Christians Introduction: There are two common ways to test for soil nutrients, the SLAN Method (Sufficiency Level o f Available Nutrients) and the BCSR method (Basic Cation Saturation Ratio). The SLAN method measures the amount o f nutrients in the soil and makes recommendations based on studies done for that particular soil type and crop. Therefore, using the SLAN method for determining the fertilizer requirements for turfgrasses can be problematic, since soil testing facilities usually make recommendations based upon agricultural row crops. The BCSR method consists o f measuring the relative quantities of cations on the cation exchange sites and comparing their ratios. It is believed that optimal plant growth can be achieved by having Calcium on 60-65% o f the cation exchange sites with ratios for the other cations being; Mg+2 10-20%, K+ 5-10%, N H / 5% and H* 5-20%. When test results for a soil are outside the ranges for a cation, fertilizer treatments are recommended to correct any deficiencies and to bring the ratios back in line. The majority o f sand used in construction o f greens and athletic fields is termed calcareous, because o f the high quantity o f calcium carbonate contained in the sand. Moreover, this sand usually, has a high pH around 8.2-8.3 and a low cation exchange capacity around 1.2 meq/lOOg. It is not unusual for calcium content to be low on cation exchange sites, resulting in recommendations for adding calcium. Calcium is recommended to be added even though the soil media is calcareous and contains a large amount o f calcium in the soil. Objectives: To discover if adding calcium to calcareous soils that have a low calcium ratio will increase the amount o f calcium taken up by the turfgrass plant, and more importantly if this increased calcium is beneficial to the grass plant. Materials and Methods: During the fall o f 1998, a preliminary greenhouse study was conducted looking at five different calcium treatments (no calcium added, gypsum, lime, calcium nitrate, and calcium chelate) applied to Kentucky bluegrass and creeping bentgrass grown on calcareous sand and silica sand. Results: During the 12-week test period some visual response trends among the different treatments were noticed, but the data is still being analyzed to determine if the treatments were significant or not. Preliminary data are listed in Tables 1 and 2. Table 2. Calcium concentration in dry tissue (ppm) for Table 1. Calcium concentration in dry tissue (ppm) for the two different soil types. the two different grasses. No Calcium Gypsum Lime Calcium Nitrate Calcium Chelate Bentgrass Bluegrass 7522 10257 9882 9478 11309 4282 4951 4680 4745 5788 No Calcium Gypsum Lime Calcium Nitrate Calcium Chelate 114 Calcareous Sand Silica Sand 7664 8675 7523 7159 8317 4140 6532 7038 7064 8781 Ornamental Studies Effect of Organic and Mineral Mulches on Soil Properties and Growth of Fairview Flame® Red Maple Trees Jeffery K. lies and Michael S. Dosmann Abstract. Five mineral mulches (crushed red brick, pea gravel, lava rock, carmel rock, and river rock) and three organic mulches (finely screened pine bark, pine wood chips, and shredded hardwood bark) were evaluated over two years to determine their influence on soil temperature, moisture, and pH, and to quantify their effect on growth of Fairview Flame® red maple (Acer rubrum L.). Mulch treatments (2.3-m2 (25-ft2) plots o f eight mulches applied as separate treatments and a non-mulched control) were randomly applied to trees in five blocks. Organic mulches were placed directly on bare ground, while mineral mulches were underlaid with a woven polypropylene fabric. Soil temperatures were highest and soil moisture percentages lowest under the mineral mulches and non-mulched control. Soil pH readings were highest under shredded bark and wood chips, and lowest in the non-mulched control. Despite these differences in root zone environments, there were no significant differences in tree height. Trees growing in river rock, crushed brick, pea gravel, and carmel rock, however, had larger stem calipers than those growing in shredded-bark plots. Crushed brick, pea gravel, and carmel rock treatments also resulted in greater leaf dry mass than shredded-bark. Leaf dry mass also was greater for trees in crushed brick and pea gravel than screened pine. Our results indicate mineral mulches used in this study do not create growth-limiting soil environments. Benefits o f using wood and bark by-products as horticultural mulch over the root zones o f landscape plants are wellestablished (Gleason and lies 1998; Green and Watson 1989; Greenly and Rakow 1995; Skroch et al. 1992; Watson 1988), however, several actual or perceived problems associated with organic mulches such as, unacceptable appearance (Rakow 1992), creation o f a temporary soil nitrogen deficiency (Ashworth and Harrison 1983), potential fire hazard (Hickman and Perry 1996), and rapid decomposition (Rakow 1992) have led to increased usage of mineral or rock mulches. But concerns that materials like rock, gravel, and crushed brick may promote potentially injurious high temperatures both above and below the mulch layer, alkalinization of the soil, and mechanical injury to the stems o f plants, have caused many landscape and tree-care professionals to reexamine their rationale for using mineral mulches as suitable ground-covering materials around woody and herbaceous plants. This experiment was designed to evaluate and compare the effects o f five mineral and three organic mulches on 1) several soil properties, and 2) growth o f Fairview Flame® red maple (Acer rubrum L.). MATERIALS AND METHODS Ninety bare-root, 1.6- to 2.0-cm (0.6- to 0.8-in-caliper), 1.2- to 1.5-m (4- to 5-ft-tall), branched Fairview Flame® red maple trees were planted in a Nicollet fine sandy loam soil at the Iowa State University Horticulture Research Station, Gilbert, Iowa (USDA hardiness zone 5a; lat. 42°3'N), on April 22, 1996. The experimental design was a randomized complete block with nine treatments, five blocks (replications), with treatments repeated twice in each replication. Trees were spaced 2.0 m (6.5 ft) apart in north-south oriented rows with 3.0 m (10 ft) between rows. Trees were hand watered once on the day o f planting to facilitate establishment. Treatments consisted o f 2.3-m2 (25-ft2) plots o f eight mulches: a 5.0-cm (2-in) layer o f 1.9-cm (0.75-in) diameter crushed red brick, 0.9-cm (0.4in) diameter pea gravel, 1.3-cm (0.5-in) diameter lava rock, and 2.5-cm (1.0-in) diameter carmel rock (chert); a 7.5-cm (3.0-in) layer o f 3.8-cm (1.5-in) diameter river rock; a 10.0-cm (4.0-in) layer o f 4.0- to 6.0-cm (1.6- to 2.4in) long finely screened pine bark, 2.0- to 3.0-cm (0.8- to 1.2-in) diameter pine wood chips, and 4.0- to 5.5-cm (1.6- to 2.2-in) long shredded hardwood bark (mostly oak); and a non-mulched control maintained as bare ground. Organic mulches were placed directly on bare ground, while mineral mulches were underlaid with a woven polypropylene fabric (DeWitt Landscape Pro 5). Weeds and other unwanted vegetation within and between treatment plots, and along the east and west borders o f the plots (15-cm wide) were controlled with glyphosate (1% v/v). Plots were not fertilized. Soil moisture was recorded weekly during the growing season (June-August) in 1996 and 1997 with a Theta Probe (meter type HH1, sensor type ML1; Delta-T Devices Ltd., Cambridge, United Kingdom) soil moisture sensor at 6 cm (2.4 in) below the soil surface. Soil temperature also was determined weekly using a portable Bamant 115 thermocouple thermometer (model 600 2810; Barrington, 111.) at 10 cm (4.0 in) below the soil surface. Both soil moisture and soil temperature readings were taken on the south side o f the tree, approximately 0.6 m (2.0 ft) from the trunk. 115 Ornamental Studies Stem diameter at 15 cm (6 in) above the soil surface and tree height from soil surface to the highest point in the crown were measured on September 19 and 20, 1997, respectively. Leaves were harvested from each tree on October 4 and 5, 1997, dried at 67°C (153°F) for five days, and weighed. Randomly chosen soil samples (one from each treatment in each replication) taken at the soil surface immediately below the mulch treatment, were retrieved on December 1, 1997 and again on June 17, 1998 to determine pH. All data were subjected to analysis o f variance and means separated by least significant difference (P < 0.05). RESULTS AND DISCUSSION Effects on soil temperature and moisture. In 1997 (data from 1996 are not presented because unseasonably cool, wet conditions caused a lack o f statistical significance), highest soil temperatures were recorded in the non-mulched control plots, followed by pea gravel, crushed brick, and carmel rock treatments (Table 1). Plots covered by organic mulch treatments had significantly lower soil temperatures (mean = 23.4°C/74.1°F) than plots treated with mineral mulches (mean = 25.9°C/78.6°F). Loosely packed organic mulches insulate soils by intercepting and absorbing solar radiation instead o f conducting heat energy downward (Waggoner et al. 1960; Montague et al. 1998). Soil moisture content was highest under the three organic mulches and pea gravel, however, the shredded-bark and pea gravel treatments were not different from lava rock or crushed brick (Table 1). Lowest moisture percentages were recorded in the non-mulched control. Soil moisture under mulch is increased through minimizing soil surface evaporation (Himelick and Watson 1990). In our study, organic mulches that meshed together, and fine-textured mineral mulches like pea gravel, presented a greater barrier to evaporation than coarser mulch materials or bare soil. Effects on soil chemistry. Previous researchers report organic mulches cause no change in soil pH (Greenly and Rakow 1995; Watson and Kupkowski 1991) or reduce pH of the underlying soil (Billeaud and Zajicek 1989; Hild and Morgan 1993; Himelick and Watson 1990). Mulch-induced pH reduction results from the addition or retention o f organic matter, with organic acids produced from decomposition o f plant-derived materials accumulating or leaching into the soil (Himelick and Watson 1990). At the completion o f our study (1997), soil pH was lowest in the non-mulched control plots and highest under shredded-bark and wood chip mulches (Table 1). Elevated pH under these mulches could have resulted from the leaching o f basic cations (N H /) from decomposing organic matter (Tisdale et al. 1993). If so, we would expect the increase in pH from ammonification to be temporary, however, because pH will decrease as ammonia is oxidized to nitrate by nitrifying bacteria in the soil. In 1998, soil pH readings again were highest under wood chip and shredded-bark mulches. Lowest pH measurements were recorded in the lava rock treatment and in the un-mulched control. While some mineral mulches could contribute to undesirably high soil pH, mineral mulches used in this study did not. Effects on tree growth. Temperature, moisture, and chemical differences in root-zone environments brought about by the various mulch treatments did not translate into differences in tree height, however, trees growing in pea gravel, crushed brick, carmel, and river rock had larger stem calipers than those growing in shredded-bark plots (Table 2). Stem calipers o f trees in the three organic mulch treatments, lava rock, and in the non-mulched control were not different. Crushed brick, pea gravel, and carmel rock treatments resulted in greater leaf dry mass than shredded-bark plots. Leaf dry mass also was greater for trees in crushed brick and pea gravel than for trees mulched with screened pine. Dry mass o f trees in the three organic mulch treatments, river rock, lava rock, and in the control were not different. Differences in tree growth are most likely linked to temperature differences in the soil environment. Although we did not measure soil temperatures in April and May, based on summer readings it is logical to assume soil temperatures under organic mulches would be cooler and possibly more growth limiting (at least for the shreddedbark and screened pine mulches) than warmer, growth-enhancing temperatures under the mineral mulches (particularly pea gravel, crushed brick, and carmel rock). Holloway (1992) reported similar results in Alaska, where five woody plant species grew best in stone mulch treatments. Elevated pH also might have contributed to poorer growth for trees in the shredded-bark treatments. 116 Ornamental Studies CONCLUSION Our results indicate mineral mulches used in this study do not create growth-limiting soil environments. In fact, the capacity o f crushed brick and pea gravel to conduct heat to soils below, particularly in early spring, may be responsible for the observed advantage in leaf dry mass for trees growing in these materials over those growing in soils kept relatively cool by insulating organic mulches such as shredded-bark and screened pine. Mineral mulches used in this study also proved to be relatively inert, causing equal or smaller increases in pH than shredded-bark or wood chips. These results, however, should not be interpreted as an indictment o f organic mulches. Because soils at the ISU Horticulture Research Station are fertile and well-drained, the organic matter and nutrient contributions made by organic mulches may be o f less consequence than if the study had been conducted on poor soils. Moreover, had conditions been drier and warmer during the years of the study (1996-97), or if the experiment had been conducted in a warmer climate, organic mulches may have outperformed many of the mineral mulches. Finally, because stem caliper and leaf dry mass measurements o f trees growing in wood chips and any o f the mineral mulches were not statistically different, blanket statements and generalizations regarding the performance of woody plants mulched with organic or mineral (rock) materials are unwise. The nursery and landscape industry is fortunate to have a wide variety o f mulch materials to choose from, and each has its place in the landscape. But in the final analysis, cost and maintenance considerations dictate which mulch materials will be used. Acknowledgments. The authors wish to acknowledge and thank the International Society o f Arboriculture Research Trust and the Iowa Nursery & Landscape Association Research Corporation for funding this research. LITERATURE CITED Ashworth, S. and H. Harrison. 1983. Evaluation o f mulches for use in the home garden. HortScience 18(2): 180-182. Billeaud, L.A. and J.M. Zajicek. 1989. Influence o f mulches on weed control, soil pH, soil nitrogen content, and growth o f Ligustrum japonicum. J. Environ. Hort. 7(4): 155-157. Gleason, M.L. and J.K. lies. 1998. Mulch matters. Amer. Nurseryman 187(4):24-31. Green, T.L. and G. W. Watson. 1989. Effects o f turfgrass and mulch on the establishment and growth o f bare-root sugar maples. J. Arboric. 15(11):268-272. Greenly, K.M. and D. A. Rakow. 1995. The effect o f wood mulch type and depth on weed and tree growth and certain soil parameters. J. Arboric. 21(5):225-232. Hickman, G.W. and E. Perry. 1996. Using ammonium sulfate fertilizer as an organic mulch fire retardant. J. Arboric. 22(6):279-280. Hild, A.L. and D.L. Morgan. 1993. Mulch effects on crown growth o f five southwestern shrub species. J. Environ. Hort. 1l(l):41-43. Himelick, E.B. and G.W. Watson. 1990. Reduction o f oak chlorosis with wood chip mulch treatments. J. Arboric. 16(10):275278. Holloway, P.S. 1992. Aspen wood chip and stone mulches for landscape plantings in interior Alaska. J. Environ. Hort. 10(l):23-27. Montague, T., R. Kjelgren, and L. Rupp. 1998. Surface energy balance affects gas exchange o f three shrub species. J. Arboric. 24(5):254-262. Rakow, D.A. 1992. Mulching: Benefits backed by survey. Arbor Age 12(9):22-29. Skroch, W.A., M.A. Powell, T.E. Bilderback, and P.H. Henry. 1992. Mulches: Durability, aesthetic value, weed control, and temperature. J. Environ. Hort. 10(l):43-45. Tisdale, S.L., W.L. Nelson, J.D. Beaton, and J.L. Havlin. 1993. Soil Fertility and Fertilizers. MacMillan Publishing Co., New York, NY. 634 pp. Waggoner, P.E., P.M. Miller, and H.C. DeRoo. 1960. Plastic mulching - principles and benefits. Bull. No. 634, Conn. Agric. Exp. Stn., New Haven. Watson, G.W. 1988. Organic mulch and grass competition influence tree root development. J. Arboric. 14(8):200-203. Watson, G.W. and G. Kupkowski. 1991. Effects o f a deep layer o f mulch on the soil environment and tree root growth. J. Arboric. 17(9):242-245. 117 Ornamental Studies Table 1. Effect o f eight mulch treatments and a non-mulched control on soil temperature, percentage (%) soil moisture, and soil pH. Temperature2 Moisturey __________PHX 1997 (°C) (%) Control 29.3Wav 19" d 6.03’ d Pea gravel 27.6 b 31 ab 6.44 b Crushed brick 26.2 c 30 be 6.29 be 26.2 c 29 c Carmel 6.29 be River rock 25.2 d 29 c 6.47 b Lava rock 24.5 d 30 be 6.21 cd Shredded-bark 23.6 e 31 ab 6.82 a Wood chip 23.3 e 32 a 6.81 a Screened pine 23.2 e 32 a 6.13 cd zSoil temperature measured at 10 cm (4 in) depth, between 2:00 and 4:00 p.m., CST. ySoil moisture measured at 6 cm (2.4 in) depth, between 2:00 and 4:00 p.m., CST. xSoil samples for pH measurements collected at 0- to 10-cm (0- to 4-in) depth. wData shown are means of 12 dates x 5 replications (n=60) in 1997. vMean separation within columns by LSD, P < 0.05. “Data shown are means of 12 dates x 5 replications (n=60) in 1997. Data shown are means of 5 observations. Soil samples collected on December 1, 1997 for pH determination. sData shown are means of 5 observations. Soil samples collected on June 17, 1998 for pH determination. Treatment 1998 5.86s d 6.14 be 6.04 cd 6.06 cd 6.33 b 5.82 d 6.81 a 6.37 b 6.14 be Table 2. Effect o f eight mulch treatments and a non-mulched control on stem caliper, height, and leaf dry mass o f Acer rubrum Fairview Flame®. Height1 Stem caliper3' Leaf dry mass1 Treatment (cm) (cm) (g) Lava rock 222w av 4.1u ab 441‘abc Wood chip 222 a 4.1 ab 423 abc Pea gravel 220 a 4.2 a 467 a 219 a Crushed brick 4.2 a 478 a Control 219a 4.1 ab 419 abc Carmel 218a 4.2 a 463 ab River rock 214 a 4.2 a 449 abc Screened pine 214 a 4.0 ab 398 be Shredded-bark 210a 3.9 b 383 c zHeight measured from ground level to highest shoot apex on September 20, 1997. yStem caliper measured 15 cm (6 in) above ground level on September 19, 1997. xLeaves harvested October 4 and 5, 1997. "Data shown are means of 10 observations. vMean separation within columns by LSD, P < 0.05. “Data shown are means of 10 observations. Each observation is the average of measurements taken at (1) the widest point on the stem, and (2) rotated 90° clockwise from the first measurement. Data shown are means of 10 observations. 118 Ornamental Studies Prairie Demonstration David D. Minner and Paul Stevens Table 1. Prairie plant identification plots. __________ Individual plots are 10 ft by 2 ft. Sideoats Tall Wild grama boneset bergamont Iowa has two broad regions for potential natural vegetation. Bluestem prairies are found in the north half o f Iowa while oak-hickory forests dominate the southern- half o f the state. Throughout the entire state there are pockets o f land that support a mixture o f both prairie and forest. The term “bluestem prairie” can be somewhat misleading since there is a wide variety o f forbes and grasses that make up Iowa’s prairie plant community. There are usually less than 10 different grasses found in most prairies, while there may be 30 to 50 different forbes or wild flowers. This demonstration area was initiated to show the diversity o f plants suitable for prairie restoration in Iowa. Furthermore, many turf managers are finding that the prairie can provide an appealing and low maintenance alternative for some turf areas. Sand love grass Mountain mint Little bluestem Purple prairie cone flw. Boneset Western wheatgrass Bottle brush Tall dropseed Individual species o f prairie plants are growing in labeled plots for easy identification. The plants were started in the greenhouse and then field transplanted as plugs in the spring o f 1997. Canada wild rye Indian grass Big blue stem Fowl mana grass North < - Blue joint grass Prairie cord grass 119 Long headed cone flw. White prairie cone flw. Purple prairie clover False dragon head Foxglove bear tongue Meadow blazing star Prairie smoke New England aster Lance leaf coreopsis Slender mountain mint Black eyed Susan Sweet black eyed Susan Prairie alumroot Yellow cone flower Introducing Iowa State University Personnel Affiliated with the Turfgrass Research Program Barbara Bingaman, Ph.D. Postdoctoral Research Associate, Horticulture Dept. Doug Campbell Research Associate, Horticulture Department Nick Christians, Ph.D. Professor, Turfgrass Science Research and Teaching Horticulture Dept. Jim Dickson Former Superintendent, Turfgrass Research Station Horticulture Dept. Mike Faust Graduate Student, M.S. (Christians - Graduated Dec. 1998) Mark Gleason, Ph.D. Professor, Extension Plant Pathologist, Plant Pathology Dept. Mark Helgeson Field Technician, Horticulture Dept. Clinton Hodges, Ph.D. Professor, Turfgrass Science Research and Teaching Horticulture Dept. Mark Howieson Graduate Student, M.S. (Christians) Jay Hudson Graduate Student, M.S. (Minner) Jeff lies, Ph.D. Associate Professor, Extension, Nursery Crops/Omamentals Horticulture Dept. Young K. Joo, Ph.D. Visiting Scientist from Korea Deying Li Graduate Student, Ph.D. (Christians and Minner) Melissa McDade Graduate Student, M.S. (Christians) David Minner, Ph.D. Associate Professor, Turfgrass Science Research and Extension Horticulture Dept. Richard Moore Superintendent, Horticulture Research Station Josh Olson Field Technician, Horticulture Dept. Gary Peterson Commercial Horticulture Specialist Rodney St. John Superintendent, Turfgrass Research Station, Horticulture Dept. Brad Sanders Field Technician, Horticulture Dept. Paul Stevens Field Technician, Horticulture Dept. Joe Stoeffler Field Technician, Horticulture Dept. 120 Companies and Organizations That Made Donations or Supplied Products to the Iowa State University Turfgrass Research Program Special thanks are expressed to the Big Bear Turf Equipment Company and Cushman Turf for providing a Cushman Turfgrass Truckster, a 15 cu. ft. Turfco topdresser, and Ryan GA30 aerifier; to Tri-State Turf and Irrigation for providing a Greensmaster 3100 Triplex Greensmower and a Groundsmaster 345 rotary mower; and, to Great American Outdoor for providing a John Deere 2243 Triplex Greensmower for use at the research area. We would also like to acknowledge Williams Lawn Seed Company of Maryville, MO for supplying a Perma Lock Inc. pesticide storage building for use at the turfgrass research area. Standard Golf Company SubAir TeeJet Spray Products Terra Chemical Corporation The Toro Company Tri State Turf & Irrigation Co. True Pitch, Inc. Turf-Seed, Inc. United Horticultural Supply United Seeds Inc. Weathermatic Corporation Williams Lawn Seed Company Zeneca Professional Products Agr-Evo USA Company Akzo Nobel Big Bear Turf Equipment Company Cushman Turf D & K Turf Products Dakota Peat DowElanco Gardens Alive Glen Oaks Country Club Golf Course Superintendents Association of America Grain Processing Corporation Great American Outdoor Heatway Hunter Industries, Inc. Iowa Golf Course Superintendents Association Iowa Professional Lawn Care Association Iowa Sports Turf Managers Association Iowa Turfgrass Institute Jacklin Seed LESCO Incorporated M. Putterman & Company Inc. Milorganite Monsanto Company Novartis Ossian Inc. PBI/Gordon Corporation Pickseed West Incorporated Profile Products Rainbird Irrigation Company Reams Sprinkler Supply Renaissance Fertlizer Co. Rhone-Poulenc Chemical Company Riverdale Chemical Company Rohm and Haas Co. The Scotts Company Seeds West Inc. SportGrass, Inc. 121 . . . and justice for all The Iowa Cooperative Extension Service’s programs and policies are consistent with pertinent federal and state laws and regulations on nondiscrimination. Many materials can be made available in alternative formats for ADA clients. Issued in furtherance of Cooperative Extension work, Acts of May 8 and June 30, 1914, in cooperation with the U.S. Department of Agriculture. Stanley R. Johnson, director, Cooperative Extension Service, Iowa State University of Science and Technology, Ames, Iowa.