2002 Iowa Turfgrass Research Report . . . and justice for all The Iow a Cooperative E xtension S ervice’s programs and policies are consistent with pertinent federal and state law s and regulations on nondiscrim ination. M any materials can be m ade available in alternative formats for A D A clients. Issued in furtherance o f Cooperative Extension work, A cts o f M ay 8 and June 30, 1914, in cooperation w ith the U .S . Department o f Agriculture. Stanley R. Johnson, director, C ooperative Extension Service, Iow a State U niversity o f Scien ce and T echnology, A m es, Iowa. 1 o O U> O & Ui Ö 1 o I Ö r* L, oo S c vo 4^ Ul O Ul 5* 3 g- r po S § S W ►fi *~ ä . 3 jf £r ö ora o Q °o a 13 S a 1 1 p r Ö Ü Ö Ö 0000 85 85 85 85 3 3 3 3 ÖO Ö r* § 5 cp * $ I 3 vo 00 00 ON U> o M U2 n o 3 § o S2 n 3 “ ® « SP 3 3. I * Ig s « i cd 3. T5 io 3 cd ^-1 t Û 5 ^ O » o 3 n CD 3 % 1 a ? 9 3 3. 3 ? 3 CD3 gl o. Xi q- 2 & cd HP33M § 0 Ä » 5 C 1 W1 c g o Ä X “ 3 S. a f °3 §3 § 7 cd CA a 3 o 0 » i CD o §••5- a 3 a » a 1 « Ä § 9 o" § l £ 2 O3 ^ 5Tï3- a §• >5 . . on V g v ; V 3- O "■h 3 2 o Ï 5* CTQ 3 3 ö 1 ? * £ £ . ; o^ S g. ö CC TQ D 60 3 § f cd ** * S a 2. rî m M ö £ . 13 cd 3 3 5' fie — * 3 £ 2 . ^ ° ^ M 2 fc> ' 3 O 3 35 “ a 3 o 85 g 7? e 3 3 CD O äT g Z tn s- 1 vT* 3 ^ S ts a ’ < CTQ < 00 L , 3 cd | S ” S q S/5 P|*T 3~ c 1o cd3 ! ï ® Ö 51 1 O « § * 3 §nT C«TQ ^ i? ^ ? g: ^ ^ EL 3 % h p a . § P P *. O g- ^ On H 3 3. « ^ ^ h a. g3 c- e< r> S a. 3. ri p “ £T w b o S ft. g . « Ö 1 r cd 5 &î w q 8 i «S- SS 3 a- ^ ^ o a a. 6s 5 f 2 . a ö p a ^ 5 ^ s a §• 3 3 Id1I. c 3 *» O) » 2 i «1 3 ge* p 3 c? & 7 1 I2. 2 cd 1D 1Q C Í? 3 ' >j ora 9 -8 » s 5 ^ ^ cd ° ^ 3 5 H % 3* 2 5* 3 30O Q Ho 2 £öL cd cd 1 ca oS \m ^ I ja s w f* s *< M 3 O 1 C a CD 3 o 3 Í » PH ^ a a 3 00 ft» V* Ö «i S O 1 113 * 8 .8 S foZ l l g ? u 2s . H^Q c fî J » i* §• o o ^ 5 ‘ § ! rV CL CD I« § ? I 2 § p§ 2. 0> oj 5 3 t>5 CD ora S' o 3 CD fl oo s 5 1 1 ^ 51 § * . ^ Ö C»1 bü § 2 a K 3 3 I ’ ^§ S" o 3 . » i CD CD § g. sS 23 3 CL 3 s t 5 m i t ? 5 I § t CD CA ? 7- _ ^ 2 3 3 3 & CD K> O K> Introduction Nick E. Christians , David D. Minner, and Shui-Zhang Fei The following research report is the 23nd yearly publication of the results of turfgrass research projects performed at Iowa State University. This is the fifth year that the entire report is available on the Internet. This report and the previous years' reports can be accessed at: http://turfgrass.hort.iastate.edu/ Several new projects were started in the 2001 season. Many of these are part of Dr. Fei's breeding related work. They include Round-up ready bentgrasses, perennial ryegrass cold hardiness screening trials, and Poa species phylogenetic studies. Dr. Fei also established a new Tall Fescue turfgrass evaluation trial in the fall of 2001. We would like to acknowledge Will Emley, superintendent of the ISU Horticulture Research Station; Rod St. John, manager of the turf research area; Barbara Bingaman, Postdoctoral researcher; Federico Valverde, research associate; Dr. Young Joo, visiting scientist; Deying Li, Postdoctoral researcher; Mark Howieson, Troy Oster, Natalie Canier, Robert Wieners, Yanwen Xiong, S.K. Lee, and Jason Kruse, graduate students; and all others employed at the field research area in the past year for their efforts in building the turf program. Special thanks to Zachary P. Kotlarek and Liying Li for helping to prepare this publication. Edited by Nick Christians, David Minner and Shui-Zhang Fei, Iowa State University, Department of Horticulture, Ames, IA 50011-1100. Dr. Nick Christians Phone: 515/294-0036 Fax: 515/294-0730 E-mail: nchris@iastate.edu Dr. David Minner Phone: 515/294-5726 Fax: 515/294-0730 E-mail: dminner@iastate.edu IV Dr. Shui-Zhang Fei Phone: 515/294-5119 Fax: 515/294-0730 E-mail: sfei@iastate.edu Environmental D ata................................................................................... 1 Species and Cultivar Trials Results of Regional Kentucky Bluegrass Cultivar Trials................................................................ 3 Fairway Height Bluegrass Trial........................................................................................................... 8 Perennial Ryegrass Studies...................................................................................................................9 National Tall Fescue Cultivar Evaluation........................................................................................ 13 Regional Fine Fescue Cultivar Trial................................................................................................. 15 Fairway Height Bentgrass Cultivar Trials........................................................................................ 18 Green Height Bentgrass Cultivar Trials........................................................................................... 19 Shade Adaptation Study..................................................................................................................... 21 Ornamental Grasses Project......................................................................................................... 23 Herbicide and Growth Regulator Studies Preemergence Annual Grass Control Study.................................................................................... 25 Postemergence Broadleaf (Violet) Study.........................................................................................31 Dimension Safety on Bentgrass/Poa annua Greens........................................................................35 Dimension + Turf Enhancer/TGR Safety on Bentgrass/Poa annua Greens Study.................... 45 Field Evaluation of Roundup Ready® Creeping Bentgrass..........................................................49 Corsair Perennial Ryegrass Control Study...................................................................................... 50 Ronstar: Demonstration of Efficacy of Various Granular Formulations...................................54 Effects of Application Timing with ZPP1560 on Spectrum of Weed Control in Turf..............59 Monsanto 78365 Demonstration for Summer Fastbum Symptomology in Turfgrass...............63 Broadleaf Herbicide Study.................................................................................................................66 Fertilizer Trials Fine Tuning Calcium Chloride - Urea Ratios for De-Icer Purposes...........................................69 Turfgrass Disease Research Brown Patch Fungicide Trial............................................................................................................. 72 Dollar Spot Fungicide Trial............................................................................................................... 73 Traffic Tolerance Research Bermuda Species Traffic Study.........................................................................................................74 Kentucky Bluegrass Traffic Study.................................................................................................... 77 Species Traffic Study..........................................................................................................................79 Environm ental R esearch 1991 Com Gluten Meal Crabgrass Control Study - Year 11 ...................................................... 81 1995 Com Gluten Meal Rate Weed Control Study - Year 7 ........................................................88 Soil M odification and Sand-based Systems Anti-Desiccant Winter Protection of Creeping Bentgrass Putting Greens..................................96 Direct Heat Stress Effects on Creeping Bentgrass..........................................................................98 Sand-Based Sport Field Stability Study......................................................................................... 100 Introducing Iowa State University Personnel Affiliated with the Turfgrass Research Program................. 104 Companies and OrganizationThat Made Donations or Supplied Products to the Iowa State University Turfgrass Research Program.............................................................................................105 v _k __ KO ) K K4) K ) LKA ) K ) K ) tO LO 00) ^ OS K) K — o S b> K O) so 00 -J os LA b lo lo > © ^OOO-vlONU^WtO- K) “O CD P <— ► D- CD OOo o o o o o o o OO© © © © © © © © © © © © © © © © I— * LA la © © © b O © © © © ©) © © © 00s to b Ö OÖ o Ö o Ö o Ö o Ö o Ö o b o o K) O LA © K) O S © la © © © K bS LbO © © © © ^ B 5' £ 3> — ■fD3g ^ 2 3 * §“3 0 00 K1) os o o s 00 -s"J LO 00 la O s 0 LA SO S LLA ■O O A OS O s -j so o0 oss 'J soo LO A© OS 0O ©0 -O OS la © b < S L0A toS L© SO KS ) OS O © b 00 © la b to U) bs b © © to bs la © 00 b b b k) b b b b b b b b ôI ■1 O P P o•1’ rr CD o 3 p 00 < -+ P CD G p. <’ CD LA P LO L1 O LA LbA LUA U) p to 00 to 0 to LO © b -b b LhA bS lo 4—U O b O ) sbo so p — 1P O LpA LbO p 0 U —LA LO LbO LbO 0 -P)* LyO 0 to) L-J * 00 b b» © U) b 00 b 00 b b b 00 00 b b b to b to b 00 b b bs b » LO sto U) © to -0to O 10 to to to to to to o 00 S to i b LO to © 3 00 © SO 00 ~o OS LA b LO to •0 23 0 *< os LA b LO to © © © © © © © P © © © © © © © © © © © © © © © © © © © © © © © b to b bs © b sto A© © © © © b © b © b b to 2 3 &3 3 3 G 1 3 "3 0 LO to tos totoi b to LO to to to to © to s to 0< sto o0 © 1o 00 'O OS LA b LO to = © so 00 "0 os LA b LO © 0 © © © © © © © © 0 © © © © © © © © © © © © © © © © © © © b b © b © b b b © © b © b © © 0Ö © © © b © b © b © b b b © b b © © © © © © © to © © b to © SO © © © SO © O b 1—1 © to 00s 00 0 s00o s0o 0o 00 -o -J -o < spo o 00 00 so 00 00 -0 -4 ^4 O 1 00 00 00 'O s o s O os 0 OS b L0A s 'O LO Os P1 L "O U) 00 CD CD j-, g b totoi 0 b0 totoi to i b b © CD C/3 CD ^ S o sr 3 S ? 3 II 3 »1 U) O CT CD K) O O NO 00 -0 On NO NO LO so Ko OO 0 0 NO OO LAl o k ON NO NO k L o OO 00 Ö OO ON ON ^1 -0 'O 00 OO -O OO ON NO Ko 0 0 NO NO kl NO 1—k Ö OO 00 00 LO lo on o Ö o o Ö o o Ö o o Ö o 00 k NO LO I— 1 o0 0 ^0 01 k k k k 00 OO O o Ö Ö o o 00 P O o Ö o o Ö O P o la i Ö Ö o On o 00 00 00 OO LO k l NO K) Ko o Ö o lai k LO N) - 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TK3 TX3 J s cd cd cd cd (D .SH o S S 5 îg ‘S 1 M O O H n vi Vi tO O O O t- H M Ifl U m Vh O O H lx O T3 > 2£cQC*Sc*íceíQ¿ c/5 cyô > U £ ï2 «3 S0 eu e u .u S •S -a 3 1 2 «P 2 S ^ ï £ ^ û U tu C M » a O ^x M O (^ N N n( vNt (i fNì '(ONM( X O (v N O -nC^SnnnTrt)' x xf ixì T x }x ' xi rx' ùxhxO^Ot SO (vN N !N CM Preem ergence Annual Grass Control Study B.R. Bingam an aE. C hristians This trial was conducted to evaluate the level of crabgrass control with several Pendulum formulations and compare Pendulum to other preemergence products. It was located at the Horticulture Research Station north of Ames, IA. The plot was established in 'common' Kentucky bluegrass with a history of crabgrass infestations. The soil was a Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll) with 3.0% organic matter, 112 ppm K, 6 ppm P, and a pH of 7.05. The experimental design was a randomized complete block. Three replications were conducted and individual plot size was 5 x 5 ft. Irrigation was used to supplement rainfall and to maintain the turf in good growing condition. The commonly-used herbicides Dimension 1EC and Pendimethalin 60WDG were included for comparisons in this trial that included numerous experimental formulations and herbicide plus fertilizer materials (Table 1). Preemergent applications were made on April 27 before crabgrass germination and the sequential application of treatment 22 was made on June 29. Granular materials were applied to dry foliage using 'shaker dispensers' to ensure uniform application. Sprayables were applied using a carbon dioxide backpack sprayer equipped with TeeJet #8006 nozzles at a spray pressure of 30-40 psi. Liquid formulations were mixed with water to a volume equivalent to 3 gal/1000 ft2. All preemergent materials were 'watered in'. Turf quality data were taken weekly from May 9 through September 13 (Tables 1 and 2). Quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. Crabgrass infestation data were taken beginning July 25 and ending on September 13 (Table 3). Crabgrass populations were estimated as the percentage area per plot covered by crabgrass. Crabgrass control was determined by converting the population data to percentage reductions as compared to the untreated control (Table 4). In addition, the study was evaluated for phytotoxicity data during the entire duration. Phytotoxicity was assessed using a 9 to 1 scale with 9 = no damage, 5 = uniform damage with some brown turf, and 1 = dead turf (Table 5). Data were analyzed using the Statistical Analysis System (SAS Institute Inc., 1989-1996) and the Analysis of Variance (ANOVA) procedure. Treatment effects on weed populations and turf quality were tested using Fisher's Least Significant Difference (LSD) test. On May 9 through May 22, quality was significantly better for turf treated with the experimental formulations with a nitrogen component (treatments 10-18) than for turf treated with other herbicides and the control (Table 1). After June 1, the nitrogen response began to fade and by June 27 there were no quality differences among the treatments. Crabgrass was first detected in the untreated turf on July 12 but was not large enough to be assessed until July 25. Crabgrass populations in treated turf were significantly lower than the untreated control through August 28 (Table 3). Mean reduction data show that all materials except BAS 656 (treatment 8) provided > 82% crabgrass control and fourteen materials resulted in > 90% control (Table 4). The preemergence application of an experimental from Gowan (treatment 21) was more effective in controlling crabgrass than the same level of the material in split applications (treatment 22). Discoloration and burning of the leaf blades were observed on turf treated with BAS 656 (treatment 8). After June 1, the phytotoxic symptoms were no longer present and the turf had recovered to a quality level similar to the untreated controls (Table 5). 25 o o o o o o o o o o o o o o o o o o o o o o O O O O O O O O O O O O O O O O O O O O O O c r- o o o o o o o o o o o o o o o o o o o o o o wSvS»/SvSuS^nvS*n«/"ivn«nw’,i vS»o«/S»/SuS*o«ri‘/Suri i n c o o o rn o o o o fo o o c n t^ r^ o o r^ m r^ o o o o v o ' o ^ o ^ 5 ' o ^ > v o ^ o ' o \ o y o ^ o t v' K r ^ ^ o ' o v o ' 0 ' o ^ o v o ro o o rn o c o o o o o c o o o o r^ r^ r^ o o m o o o oo Visual quality1 of Kentucky bluegrass treated for the 2001 Preemergence Annual Grass study (data through July 11). 3 CM ^ ' ¿ ^ ^ v o ' o \ o v o \ o ' o t ^ t ^ o o v o r ^ v o ' £ ) ' r s> ’ '0'0'0'o coroOfor^r^OOroOror^OcnrnrOfor^-fnOrnt^ v o v o ' Ov o v o v o v o v o v o r ^ o o o o o N o o o o t ^ r ^ r ^ ' o v o v o ' O yO yO /-- >. 3c3 s \ oN oN v? Ctf) .c *s 1 é éé é éé ^ J g tìw tì SO U'i'tOOMONO\000.2” ~ ~ < *> C c c so S comCMCMCMCMOOO O O O O O O — — O C 03 03 C 3 ^ - o o o o o o o o o o D 0-, Table 1. o *— iffM (N<'M Cci cC r— cm"T rnOM ^ ì x o h Cu c u Dh PQGh X « £ S2 2 JQ J QJ —Q X X X X X X X X Q cqcqcq — C M m ^ ^ v c t ^ o o o O — cMcc T f w ^ v o r ^ o o o N O —' C 3 *e+* M WO .23 .2 o §< : ‘u 0) "C o ^— fl y S £ g o w O i; o w a> q q q q q q q q q q q q q q q q q q q q q q ^oo q q q q q q q q q q q q q q q q q q q q q q 00 O s O s O O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O O s O s O s O s O s O s O s O O s cn o’ O s O s q q q q q q q q q q q q q q q q q q q q q q Table 2. Visual quality1 of Kentucky bluegrass treated for the 2001 Preemergence Annual Grass study (data through September 13). O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s O s q q q q q q q q q q q q q q q q q q q q q q GO 3 ~ < O s 5 os oooofxitxiooooooixiodooooooooodoooooooooooooooo © © 'OVOVOVOVOVOVOVOVOVOVOVOVOVOVOVOVOVOVOVOVOVO CN ON CN 7 © © © O O © o O o o * n o o » o o o i n o o » r > o < r > © © ^ vr> o m o «cn o v i n - < N « - < ( N ^ ( N - ( N » n c s ( N n - ¡{ N -itN -rj^ ^ d o o d d d o d ^ d o N ^ -O o £ o lit 13 oo o < c/a < § £ o o£ q a o .o *-* «4-1 O n ^3 .2 ‘C C ca 6 G0 OC c3 a a V V«O\° o'v\° 0s \° 0s-\° 0s" \e 0s ^ £ ^ Co o *§ © © © © © ©w so .£ Tf CO © ^ ON ON V O T f OO OO O ON © © ON CO CN CN CN CN © © © —' © © © © © © © ©© © © © -H GO a u ^ W c w W UJ _0 N- N*C33 cCd cCd Cd c C/3 C /2 C c(I) fl co rt/5 C cd J C cd GO 2 ©© 00 "O © Cfl - rl - rX,rXi <^Uf^ < ! £ Uh Uh tU E-Lh Uh Uh U- . 5 o w u D a,G,2fl-DHOHfflcuXXXXX X X Q CQ CQ CQ £ .2 po £5 - c N r i T f i n v o K o o o N O - c Nc o ^ v o ' o r ^ o o o N©- - HCN § E cr m «4¡ Q-1 «« rv oOn 3 JS N j= H H ° H © © CN rCN a> 2 '^Or^OOTfrnOOOCNmt^OO% jo o m r- o © m >o m rn n 6 (N ^ o 3 roOO O O OO w ^O OO O O -^O ON d ; x © © © o © 4+ m o m © *r> CN < CN CN © o c v - > o o » /'s o o m o o m © « /i© © - r N^ ( N^ ( N^ < NWOCNCNc n ^ O O O O O O O O —OOsK o r— Vv° /-W^ N.O o' N=> VcO ^r - r^- ^— ^— ^r ^ r^- £ £ S: o c • O O■« s o *2 o o o o o o O n O n NO c w w w vo OO 00 ON O n O O Os O T j - T j - T f vn ^g ^. . rTfo CN CN CN CN O O O '55 c e c a/2 aC/3 vo .£ o o o o o o — — O c cs C/3 C 00 •o o o o o o o p o p O C O «« 5 00 >> . -O [in < s *3 c/o £o £o o < •i ffl Qa O'h— i T<3D a) T3 •a c C cC 5 C 00 03 X «5 | Ì 2o 3C < -2 .2 o < u 00 OS 00 CO00 O __ Os OS CN v> cn 00 VS 00 so CO0 00 Os CO00 Tp Tp so Os CN 0 00 Os Os Os 00 Os Tp os Os os Os Os 00 OS 00 Os 00 Os os Os 00 CN ON C O, ro C/D 0 so r- Tp vs vs vs Tp Os OS r- Tp CNCN r- 00 vs os ',p O OOO s Tps 00 Os Os 00 0 Tp* Tp Os Ov so Os Tp* so VOOs Os so 0 00 c- OS CN Os Os OOOOOS 00 Os 00 00 OOOOos OS p^ CN Tt CN ?< M O CNc*s Os CN so 0 cn 00 OOO r- rp CN os p- TP ^p vs so Os CNso so Os SOcn os CN CO Os Os Tp Os VOVOTP so OS Tp OO OOs Os Os OOOs Os OS Os Os Os OOOS 00 OS 00 OS OS OS 00 r0 CN O COos 00 COCO 00 TP CN CN COCNos COc^- ^p COCO 00 00 00 OK Oso 00 0 Os vs Os C OO0 S OS vs os CN O OO s 00 Os C ON S0 Os 00 Os 00 C OOV S 00 OS 00 C OOvs s Os 0 O0 S 00 CO C CN N 0 —* —* — - CNso Os —Hso so os 1—1^p cn so O so CN vss CN O NO CN vss O 0 C 00 00 CN vsS O OOO s 00s V CN as SO SO Os CN s0 VS 00s Os O 0 Os 00 C ON s OS O OS s0 O0 SO O CN OO 2 < bo Table 4. Percentage crabgrass reductions1 in Kentucky bluegrass treated for the 2001 Preemergence Annual Grass study. < b£). O )0_ < ^ o bO < bo < 0 CN CN^P TP CNTP 0 CN ^P TP CN0 CN O ^p CNCNCN Tp ^p O VS TP O TP ^1* 00 so Os Tp TP 0 cn' 0 vs 0 OS O Oss OO 00 OO sO s S Os O s vs OS Os Os 00 0 Os os Os OS Os O Os Os 00 CN CN O vs vs cn p 0 so 0 cn O p cn p O O cn 0 0 V) s 0 00 Tp" 00 OS 0 00 TP OS00 cn 00 Os d cn Os d d OO OOO s OS Os OOOs TP 0 Os Os Os Os 00 Os 00 Os 00 Os Os 0 Os Tp Tp* CN O p SOso OOO00 OO OS Os os Os OS Os p p p O 00 p 00 00 O O p p 00 00 00 C CN 00 00 Os Os Os O OOd s0 O SO S ON s Os C ON Sd 0 d 0 Os c- o C N OS CN CN CN 3fc Os Tp CN K —; < £ JO 3 00 v> 00 V 00 vs 00 »0 0 vs so0 soO OO OO O n 0 Vi O p O p >—1 CN O CN CN*— ' CN p CNCNcn CN CN CN CM d d d O d d d d —- d as JO (N (N (N (N (N r^i ro D Ox cu a. a- a- cu o s a" a> 03 -o C o P > o °Vi rjr> Vi P ec C O a a. .g 13 rr'O'TT 'O'* c r - r - ----- c- r- • 2 0 0 0 0 0 0 ^ c22 o§ & •2 rj . os £ Vi e #o to o T3 O 2S 203 3o a £ £ -O & 83 8 oV V o Q c . oc o dc/>3 o *7 T3 E ~ - c N c n T p v s s o t ^ o o o s O ’' -«cNcnT3-tr>soc>' Ooaso~*cN — — C NC NCN , .2 u r~ JoOJ F = 0.0377). Those violets receiving the sequential treatment on June 28 recorded higher levels of damage than those treated only once on June 7. There were no statistical reductions in the number of violets in the treated turf as compared with the untreated turf (Table 2). There were numerical differences between the violet counts among the main and subplot treatments but they were not statistically significant. By August 3, violets in the untreated areas were dying because of the high temperatures and low rainfall. As a result, treatment effects are hard to interpret from the data (Table 3). Mean counts show that there were numerical reductions as compared with the untreated controls but the differences are not statistically significant. 31 Table 1. Violet damage1 in turfgrass treated for the 2001 PBI Gordon Postemergence violet study. Material 1. Is. 2. 2s. 3. 3s. 4. 4s. 5. 5s. 6. 6s. Untreated control Untreated control EH 1382 EH 1382 EH 1381 EH 1381 EH 1383 EH 1383 Trimec Classic Trimec Classic Millenium Ultra Millenium Ultra Rate pts/A Number of applications June 11 June 18 June 21 July 3 July 11 July 18 Mean NA NA 5.5 5.5 5.0 5.0 4.0 4.0 4.0 4.0 2.5 2.5 NA NA 1 2 1 2 1 2 1 2 1 2 9.0 9.0 4.7 4.7 5.0 5.0 4.7 4.7 7.0 7.0 6.3 6.3 9.0 9.0 5.3 5.3 5.3 5.3 5.7 5.7 5.7 5.7 5.0 5.0 9.0 9.0 5.7 5.7 5.3 5.3 5.7 5.7 6.3 6.3 6.3 6.3 8.0 8.0 4.3 3.7 4.0 4.7 3.0 3.3 4.7 4.0 3.7 3.7 9.0 9.0 6.3 5.0 4.3 5.3 5.7 6.3 6.3 5.0 5.0 4.3 5.3 4.7 6.3 6.0 6.0 3.7 5.7 3.7 6.0 3.7 5.0 2.7 8.2 8.1 5.4 5.1 5.0 4.9 5.1 4.9 6.0 5.3 5.2 4.7 2.2 2.4 1.2 2.6 1.6 NS 0.9 LSD0.05 1Damage was assessed using a scale from 9 to 1 with 9 = no damage, 8 = slight discoloration, leaf cupping, and/or stem curling, 7 = more uniform symptoms from #8, 6 = all symptoms of #7 plus leaf mottling, 5 = all symptoms of #6 plus browning on leaves, 4 = all symptoms of #5 plus some dead leaves, 3 = more uniform symptoms of #4 with more dead leaves, 2 = all symptoms of #3 plus some dead plants, and 1 = all violets dead within the plot. The initial applications were made on June 7 to the entire 5 x 10 ft plot. The sequential applications applied 21 days later on June 28 were made to a 5 x 5 ft subplot. NS = means are not significantly different at the 0.05 level. 32 Table 2. Violet counts by subplot treatment1 in turfgrass treated for the 2001 PBI Gordon Postemergence violet study. Material 1. Is. 2. 2s. 3. 3s. 4. 4s. 5. 5s. 6. 6s. Untreated control Untreated control EH 1382 EH 1382 EH 1381 EH 1381 EH 1383 EH 1383 Trimec Classic Trimec Classic Millenium Ultra Millenium Ultra Rate pts/A Number of applications July 24 August 3 NA NA 5.5 5.5 5.0 5.0 4.0 4.0 4.0 4.0 2.5 2.5 NA NA 1 2 1 2 1 2 1 2 1 2 32.7 36.7 24.0 17.3 21.0 23.7 22.0 13.0 20.0 20.3 17.3 8.7 19.7 24.7 14.7 12.3 14.0 13.0 13.3 10.0 13.3 9.7 10.3 6.0 August 9 13.0 18.0 12.7 12.7 12.7 10.3 11.7 8.7 9.0 8.3 8.7 5.3 August 14 10.0 15.0 11.3 13.7 10.3 8.7 10.7 4.7 5.3 7.0 7.0 4.7 August 24 14.7 18.3 8.0 11.0 10.7 9.0 12.0 7.3 12.0 7.0 9.7 2.7 August 31 8.7 10.0 7.7 10.3 10.3 8.7 8.0 8.7 8.0 8.3 9.3 3.7 NS NS NS NS NS NS LSD0.05 1These figures represent the number of violets per subplot. The initial applications made on June 7 were to the entire 5 x 10 ft plot. The sequential applications applied 21 days later on June 28 were made to a 5 x 5 ft subplot. NS = means are not significantly different at the 0.05 level. 33 Mean 16.4 20.6 13.1 12.9 13.2 12.2 12.9 8.7 11.3 10.1 10.4 5.2 NS Table 3. Violet counts by main plot treatment1 in turfgrass treated for the 2001 PBI Gordon Postemergence violet study. Material Rate pts/A July 24 August 3 NA 5.5 5.0 4.0 4.0 2.5 34.7 20.7 22.3 17.5 20.2 13.0 22.2 13.5 13.5 11.7 11.5 8.2 August 9 August 14 August 24 August 31 Mean 15.5 12.7 11.5 10.2 8.7 7.0 12.8 12.5 9.5 7.7 6.2 5.8 16.5 9.5 9.8 9.7 9.5 6.2 9.3 9.0 9.5 8.3 8.2 6.5 18.5 13.0 12.7 10.8 10.7 7.8 NS NS NS NS NS NS L S D q.05 1These figures represent the number of violets per main plot. The initial applications made on June 7 were to the entire 5 x 10 ft plot. The sequential applications applied 21 days later on June 28 were made to a 5 x 5 ft subplot. NS = means are not significantly different at the 0.05 level. NS NS 1. 2. 3. 4. 5. 6. Untreated control EH 1382 EH 1381 EH 1383 Trimec Classic Millenium Ultra 34 - Dimension Safety on Bentgrass a n n u a Greens B.R. Bingaman, T. R. O ster, and N. E. C hristians These field trials were designed to measure the quality of creeping bentgrass on sand and soil-based greens following treatment with dithiopyr at three different rates. Two studies were conducted at Veenker Golf Course in Ames, IA on creeping bentgrass practice greens infested with Poa annua. One study was established on a sand-based and another on a soil-based green. Individual plot size was 5 x 5 ft and three replications were run. For both studies, Dimension (Dithiopyr) was applied at 0.25, 0.50 and 1.00 lb a.i./A on May 1, 2001. The material was applied using a 'shaker dispenser' to ensure uniform coverage. The studies were monitored for turf quality and phytotoxicity from May 15 through September 5 (Tables 1 and 2). Turf quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable and 1 = worst quality. No phytotoxicity was observed. Control of P oa annuawas recorded from May 15 through September 5 (Tables 3 and 4). Populations were determined by estimating the percentage of area per plot covered by Poa annua. The small population on the soil-based green died out and/or was overgrown by bentgrass beginning mid July. Some regrowth was observed on September 5. To quantify possible treatment effects on Poa annua seedhead formation, differences in the percentage of Poa annua seed head formation per plot were noted for the sand-based green study on May 15, June 15, July 11, and July 24 (Table 5). The Poa annua on the soil-based green did not produce seed heads. The soil-based green was core aerified on August 15 and large cores (approximately 3/4" diameter) were removed. There were obvious differences in recovery from the aerification and these were noted on August 24 and August 28 (Table 6). Recovery was assessed using a 9 to 1 scale with 9 = 100% recovery, 5 = 50% recovery, and 1 = 0% recovery. Data were analyzed using the Statistical Analysis System (SAS Institute Inc., 1989-1996) and the Analysis of Variance (ANOVA) procedure. Treatment effects on Poa annua populations and visual quality were tested using Fisher's Least Significant Difference (LSD) means comparison test. In addition, orthogonal contrasts were conducted using the General Linear Model (GLM) procedure to provide means comparisons among individual treatments (Tables 7-10). Sand-based Green: No phytotoxicity was observed on the treated bentgrass. Enhanced turf quality was observed in bentgrass treated with Dimension at 0.25 lb a.i./A on May 15 and May 23 as compared with the other treated and untreated bentgrass (Table 1). On June 6, bentgrass treated with either Dimension at 0.50 or at 1.00 lb a.i./A had better quality than the other treated and untreated turf. After June 6, quality was similar for all treated and untreated bentgrass. There were numerical reductions in Poa annua cover among the treated and untreated plots, but the differences were statistically significant for only June 6 and August 15 (Table 3). On these dates, Dimension at 1.00 lb a.i./A provided the best numerical control but the level of control was not different from that caused by the other Dimension treatments. There were significant differences in percentage Poa annua seedhead formation for June 15, July 11, and July 24 (Table 5). On June 15 and July 11, there were less seedheads in bentgrass treated with Dimension at 0.25 lb a.i./A but there were more in bentgrass treated with Dimension at 0.50 and 1.00 lb a.i./A as compared with the untreated controls. Mean data show that there was significantly more seedhead formation in bentgrass treated with Dimension at 0.50 or 1.00 lb a.i./A than in bentgrass treated at 0.25 lb a.i./A or untreated. The orthogonal contrasts provide additional information on statistical differences in Poa annuacover by giving probabilities of > T values for pairwise comparisons among the treatments (Table 7). On June 1, June 15, August 15, and August 24 the differences between percentage cover in untreated bentgrass were statistically different than in bentgrass treated with Dimension at 1.00 lb a.i./A. Soil-based Green: No phytotoxicity was observed on the treated bentgrass. Numerical improvements in turf quality were recorded through September 5 but these differences were statistically significant for only June 1, June 6, and June 15 (Table 2). On these dates, turf quality was best for bentgrass treated with Dimension at 1.00 lb a.i./A as compared with the other treated and untreated areas. There were reductions in Poa annua cover in treated bentgrass as compared to untreated but the differences were not statistically significant (Table 4). The Poa annua population on this green was rather sporadic and appeared to die out by early August. In addition, the population within the experimental area and surrounding it did not form seedheads. Core aerification performed on August 15 resulted in significant differences in recovery rates (Table 6). On August 24, bentgrass treated with either Dimension at 0.50 or 1.00 lb a.i./A had significantly less recovery from aerification By August 28, the cores were still only 75% regrown in bentgrass treated with Dimension at 1.00 lb a.i./A. The cores in all other treated and untreated areas had completely recovered. 35 Table 1. Turf quality1 of creeping bentgrass on a sand-based green treated for the 2001 Dimension Safety study. 3 ’ p o o p Q\ On On 3 ^ o m ^ n oo oc C oj3Uvn C Y1 C P-N 3 < o m K h h oo £?°0 < « C O cn o o On On 3 pppp rn p r-; vo vo vo oc _ S3 < ~ p o o p OO* 00 0 0 oo 3 2 p o o p 00 00 00 00 oc < m p o o p 00* OO* OO 00* X1Tt 3 ; P oo’ 00 OO On co © co © 00 00 00 00 00 00 OO 00 < m p© nan o cO • (N IA O C Od *° JO 5^3 ¿3 — CO IANT3 © ox° ox Tf Tf 0sTf VC VO VO S° 0s- N° cN TT Tt Tt VO vo VO cO (N CO •s o o o _H I ■ I Oi rni m t— i im ° o ''c o' 32 *.2Cfl 3.2 *5« .2 8 g g g § e a e *c 6 CO 2 co co co i i i o o o .2 C /2.2C /3.2C /5 g g g aaa 5 5 5 5 5 5 — S o C oc o So C/3 -ri S3 § 2«j2 .2 ^ ? 3 £ & CO C/5 § •1 •c § p 03 1 1 |CO oE Cfl O 3 < CO CO CO O co’ 00 CO O (N M COCO © VO OV VO CO (N fS (N cG _o-CM r* c- o r- OO W O 00 < ™ O’ m O' m ©© ©wo vo Percentage Poa annua cover1 in creeping bentgrass on a sand-based green treated for the 2001 Dimension Safety study. w~i co O' co ©00 oo CO ^ Tt © ©. wo’ © VO 00 U h co © § VO o r- r- o o' t-" a C O NV CO MC P CO CO r*; CO CMCMCO © ’ 00 00 vo C00O CCO O O ©C©O C00 CMCO CO O O h; Cl O Co’MC©O C—O’ CCO e3 (V ■ .O «5 00 m r—© © co woMoWOoO s C I § I Xio 4) 03 c* S oo> o a T3 4> © © C3 •C8 s c3 03 N ® oNx=> N oN ox= Tf Tf Tf N NxO S oxO o oxO 0- 0 - 0 - VO VO VO VO vo VO © © © /—“v /—S o o o I I I CO I CIO C IO o o o O w' >>-✓ ii G C G ’g .9 .2 .2 B B Table 3. vo W O © © VO G §2 p © © © < " iri ur> ‘ 00 © © © /«“*S✓—V/— ■ s © © © _ I I I O co co co Vi I I I 0o -G a> c © © © o o o c G c mo _ o o "cfl ’cfl S5 03 c C C 4> 4) a> 1 E e 6 5 3 3 3 d 3 3 3 <— BC oX 4> © ~ OfN C to _r 8 S « - S ICfl O 4) E4)G a- c3 CCa./33 h- BC — O wnT3 p 0 o w « -C _ CO 5 V ox© s© ox sP ox ^VO VO V TfO o 3 £ N ox©N ox©N ox® i Tf ^ ^ VO vo VO C 0 s O rn cn cn £ C o -2 c« 73 c« p o o o § O O C c c c o *8 _ -2tfl .2w tfl Q Q d' B B B D Q Q Q 8 §§S cn m *— cn m Tf I ,u > • fc 3 I^ ^ c ¡0 ^ 2 !1 ®,, C g 7£3 i r rtO fi to & s .§> 1 rr* O § z t •O c §, E & =a g o Oh 7630 O CO c O) “ 2S •S E | « II E «5 !/3 11 2 Z I Table 5. Percentage Poa annua seedhead formation1 in creeping bentgrass on a sand-based green treated for the 2001 Dimension Safety study. Material 1. 2. 3. 4. Untreated control Dimension (10-3-10) 0.164% a.i. Dimension (10-3-10) 0.164% a.i. Dimension (10-3-10) 0.164% a.i. Rate lb a.i./A May 15 June 15 July 11 July 24 Mean NA 0.25 0.50 1.00 36.7 20.0 31.7 38.3 25.0 10.0 46.7 35.0 0.0 3.3 20.0 50.0 6.7 18.3 18.3 26.7 17.1 12.9 29.2 37.5 9.6 14.2 12.0 NS 5.8 LSDo.o5 1These data represent the percentage of Poa annua^XavAs per plot that were forming seedheads. Materials applied on May 1, 2001 NS = means are not significantly different at the 0.05 level. Table 6. Recovery from core aerification1 in creeping bentgrass on a soil-based green treated for the 2001 Dimension Safety study. Material 1. 2. 3. 4. Untreated control Dimension (10-3-10) 0.164% a.i. Dimension (10-3-10) 0.164% a.i. Dimension (10-3-10) 0.164% a.i. Rate lb a.i./A August 24 August 28 Mean NA 0.25 0.50 1.00 8.0 7.3 7.0 3.3 9.0 9.0 9.0 7.3 8.5 8.2 8.0 5.3 0.6 0.9 L S D 0 .o5 ^ h e recovery from aerification was assessed using a 9 to 1 scale with 9 = full recovery, 5 = 50% recovery, 1 = no recovery. Materials applied on May 1, 2001 40 0.6 . Table 7. Probabilities for > T comparing percentage Poaannuacoszx in creeping bentgrass on a sand-based green treated for the 2001 Andersons Dimension Safety Study. Material Untreated control vs treatments Untreated control vs Dimension @0.25 lb a.i./A Untreated control vs Dimension @0.50 lb a.i./A Untreated control vs Dimension @1.00 lb a.i./A Dimension @0.25 lb a.i./A vs untreated & other treatments Dimension @0.25 lb a.i./A vs untreated control Dimension @0.25 lb a.i./A vs Dimension @0.50 lb a.i./A Dimension @0.25 lb a.i./A vs Dimension @1.00 lb a.i./A Dimension @0.50 lb a.i./A vs untreated & other treatments Dimension @0.50 lb a.i./A vs untreated control Dimension @0.50 lb a.i./A vs Dimension @0.25 lb a.i./A Dimension @0.50 lb a.i./A vs Dimension @1.00 lb a.i./A Dimension @1.00 lb a.i./A vs untreated & other treatments Dimension @1.00 lb a.i./A vs untreated control Dimension @1.00 lb a.i./A vs Dimension @0.25 lb a.i./A Dimension @1.00 lb a.i./A vs Dimension @0.50 lb a.i./A May 15 May 23 June 1 June 6 June 15 June 24 June 29 July 3 July 11 0.1851 0.9983 0.0486 0.0610 0.0915 0.2731 0.8425 0.1009 0.0883 0.8376 06140 0.1961 0.5102 0.1085 0.2231 0.7463 0.3153 0..0252 0.1377 0.7351 0.1107 0.1308 0.4796 0.8284 1.0000 0.0710 0.1485 0.0761 0.8652 0.0355 0.0198 0.0645 0.2231 0.8710 0.1944 0.1070 0.1145 0.4186 0.9242 0.2896 0.3293 0.3529 0.7422 0.8841 0.7879 0.1024 0.4096 0.6922 03343 0.3014 0.3007 0.7463 0.3153 0.8207 0.1024 0.4096 0.6922 0.3343 0.3014 0.3007 0.7463 0.3153 0.8207 0.0567 0.5050 0.2590 0.0498 0.7193 1.0000 0.8710 0.7275 0.6525 0.2438 0.5860 0.5845 0.6031 0.4650 0.4344 0.8425 0.1537 0.5296 0.1377 0.7351 0.1107 0.1308 0.4796 0.8244 1.0000 0.0710 0.1485 0.1024 0.4096 0.6922 0.3343 0.3014 0.3007 0.7463 0.3153 0.8207 0.6836 0.8652 0.4378 0.2111 0.1824 0.3007 0.8710 0.4927 0.8207 0.0931 0.7857 0.1002 0.0293 0.1412 0.3468 0.9460 0.6796 0.3318 0.0761 0.8652 0.0355 0.0198 0.0645 0.2231 0.8710 0.1944 0.1070 0.0567 0.5050 0.2590 0.0498 0.7193 1.0000 0.8710 0.7275 0.6525 0.6836 0.8652 0.4378 0.2111 0.1824 0.3007 0.8710 0.4927 0.8207 41 Table 8. Probabilities for > T comparing percentage Poa annua cover in creeping bentgrass on a sand-based green treated for the 2001 Andersons Dimension Safety Study. Material Untreated control vs treatments Untreated control vs Dimension @0.25 lb a.i./A Untreated control vs Dimension @0.50 lb a.i./A Untreated control vs Dimension @1.00 lb a.i./A Dimension @0.25 lb a.i./A vs untreated & other treatments Dimension @0.25 lb a.i./A vs untreated control Dimension @0.25 lb a.i./A vs Dimension @0.50 lb a.i./A Dimension @0.25 lb a.i./A vs Dimension @1.00 lb a.i./A Dimension @0.50 lb a.i./A vs untreated & other treatments Dimension @0.50 lb a.i./A vs untreated control Dimension @0.50 lb a.i./A vs Dimension @0.25 lb a.i./A Dimension @0.50 lb a.i./A vs Dimension @1.00 lb a.i./A Dimension @1.00 lb a.i./A vs untreated & other treatments Dimension @1.00 lb a.i./A vs untreated control Dimension @1.00 lb a.i./A vs Dimension @0.25 lb a.i./A Dimension @1.00 lb a.i./A vs Dimension @0.50 lb a.i./A July 24 Aug 3 Aug 10 Aug 15 Aug 24 Aug 28 Sept 5 Mean 0.2798 0.8115 0.3158 0.0245 0.1388 0.1509 0.7773 0.4979 0.4198 0.7811 0.5087 0.0340 0.1880 0.3153 0.8349 0.4486 0.7825 0.7811 0.5087 0.1795 0.5742 0.3153 0.7814 0.8483 0.1340 0.2891 0.2530 0.0229 0.0828 0.1177 0.8349 0.4807 0.8311 0.4970 . 0.9618 0.1920 0.5026 0.8943 0.9551 0.5712 0.5847 1.0000 1.0000 0.2699 0.4072 1.0000 0.9445 0.5638 0.5847 1.0000 1.0000 0.2699 0.4072 1.0000 0.9445 0.5638 0.4198 0.1963 0.5945 0.7715 0.5742 0.4927 1.0000 0.9551 0.4999 0.4970 0.9618 0.6284 0.4881 0.8943 0.8655 0.7058 0.7825 0.7811 0.5087 0.1795 0.5742 0.3153 0.7814 0.8483 0.5847 1.0000 1.0000 0.2699 0.7072 1.0000 0.9445 0.5638 0.1990 0.1963 0.5945 0.1795 0.1880 0.4927 0.9445 0.6009 0.1498 0.1474 0.3685 0.0946 0.1402 0.2296 0.9558 0.6283 0.1340 0.2891 0.2530 0.0229 0.0828 0.1177 0.8349 0.4807 0.4198 0.1963 0.5945 0.7715 0.5742 0.4927 1.0000 0.9551 0.1990 0.1963 0.5945 0.1795 0.1880 0.4927 0.9445 0.6009 42 Table 9. Probabilities for > T comparing percentage Poa annua cover in creeping bentgrass on a soil-based green treated for the 2001 Andersons Dimension Safety Study. Material Untreated control vs treatments Untreated control vs Dimension @0.25 lb a.i./A Untreated control vs Dimension @0.50 lb a.i./A Untreated control vs Dimension @1.00 lb a.i./A Dimension @0.25 lb a.i./A vs untreated & other treatments Dimension @0.25 lb a.i./A vs untreated control Dimension @0.25 lb a.i./A vs Dimension @0.50 lb a.i./A Dimension @0.25 lb a.i./A vs Dimension @1.00 lb a.i./A Dimension @0.50 lb a.i./A vs untreated & other treatments Dimension @0.50 lb a.i./A vs untreated control Dimension @0.50 lb a.i./A vs Dimension @0.25 lb a.i./A Dimension @0.50 lb a.i./A vs Dimension @1.00 lb a.i./A Dimension @1.00 lb a.i./A vs untreated & other treatments Dimension @1.00 lb a.i./A vs untreated control Dimension @1.00 lb a.i./A vs Dimension @0.25 lb a.i./A Dimension @1.00 lb a.i./A vs Dimension @0.50 lb a.i./A May 15 May 23 June 1 June 6 June 15 July 3 July 11 July 16 0.2398 0.2773 0.2612 0.6623 0.5676 0.7454 0.7454 0.4825 0.3190 0.3351 0.3517 0.2948 0.6721 1.0000 1.0000 0.3903 0.4962 0.4694 0.5260 0.7152 0.5748 1.0000 1.0000 0.3903 0.2180 0.3123 0.2270 0.7152 0.6721 04454 04454 1.0000 0.6585 0.6270 0.7003 0.2082 0.9085 0.7454 0.7454 0.4762 0.7297 0.7920 0.7480 0.4732 0.8870 1.0000 1.0000 1.0000 0.7297 0.7920 0.7480 0.4732 0.8870 1.0000 1.0000 1.0000 0.7818 0.9578 0.7480 0.1768 1.0000 0.4454 0.4454 0.3903 0.9046 0.9520 0.8900 0.8751 0.7302 0.7454 0.7454 0.4762 0.4962 0.4694 0.5260 0.7152 0.5748 1.0000 1.0000 0.3903 0.7297 0.7920 0.7480 0.4732 0.8870 1.0000 1.0000 1.0000 0.5387 0.7520 0.5260 0.4732 0.8870 0.4454 0.4454 0.3903 0.3813 0.5668 0.3741 0.3169 0.9099 0.3559 0.3559 0.4762 0.2180 0.3123 0.2270 0.7152 0.6721 0.4454 0.4454 1.0000 0.7818 0.9578 0.7480 0.1768 1.0000 0.4454 0.4454 0.3903 0.5387 0.7520 0.5260 0.4732 0.8870 0.4454 0.4454 0.3903 43 Table 10. Probabilities for > T comparing percentage Poaannuacowc in creeping bentgrass on a soil-based green treated for the 2001 Andersons Dimension Safety Study. Material Untreated control vs treatments Untreated control vs Dimension @0.25 lb a.i./A Untreated control vs Dimension @0.50 lb a.i./A Untreated control vs Dimension @1.00 lb a.i./A Dimension @0.25 lb a.i./A vs untreated & other treatments Dimension @0.25 lb a.i./A vs untreated control Dimension @0.25 lb a.i./A vs Dimension @0.50 lb a.i./A Dimension @0.25 lb a.i./A vs Dimension @1.00 lb a.i./A Dimension @0.50 lb a.i./A vs untreated & other treatments Dimension @0.50 lb a.i./A vs untreated control Dimension @0.50 lb a.i./A vs Dimension @0.25 lb a.i./A Dimension @0.50 lb a.i./A vs Dimension @1.00 lb a.i./A Dimension @1.00 lb a.i./A vs untreated & other treatments Dimension @1.00 lb a.i./A vs untreated control Dimension @1.00 lb a.i./A vs Dimension @0.25 lb a.i./A Dimension @1.00 lb a.i./A vs Dimension @0.50 lb a.i./A July 24 Aug 3 Aug 10 0.6505 0.4762 0.4762 1.0000 1.0000 1.0000 0.5419 0.3903 0.3903 0.6236 0.3903 0.3903 0.6505 0.4762 0.4762 0.5419 0.3903 0.5419 Sept 5 Mean 0.2743 0.8327 — 0.4881 0.9249 — 0.1901 0.9474 — — 0.4881 0.6341 — — 1.0000 0.9537 0.3903 0.4881 0.8728 0.3903 0.3903 0.4881 0.8728 0.6236 0.3903 0.3903 1.0000 0.7010 0.5854 0.4825 0.4825 0.2743 0.7507 0.5419 0.3903 0.3903 0.1901 0.9474 0.5419 0.3903 0.3903 0.4881 0.8728 0.9014 1.0000 1.0000 0.4881 0.5894 0.7282 0.4825 0.4825 0.9931 0.5688 0.6236 0.3903 0.3903 0.4881 0.6341 0.6236 0.3903 0.3903 1.0000 0.7010 0.9014 1.0000 1.0000 0.4881 0.5894 44 Aug 24 — Aug 28 — — — Dimension + Turf Enhancer/TGR Safety on Bentgrass/P^a annua Greens Study B. R. Bingaman, T. R. Oster, and N. E. Christians This study was designed to measure turf quality and Poa annua population reductions on Bentgrass/Poa annua greens treated with paclobutrazole and dithiopyr concurrently. This study was conducted at Veenker Golf Course in Ames, IA on a sand-based practice green heavily infested with Poa annua. In the study area, the percentage cover of Poa annua was approximately 60%. Individual plot size was 5 x 5 ft and three replications were run. Two fertilizer/herbicide treatments and an untreated control were included. One treatment consisted of Dimension 0.164% (10-3-10) applied concurrently with Turf Enhancer 0.13% (14-0-28) on May 1 and Turf Enhancer applied alone at 28 day intervals on June 8 and June 28, 2001. For the second treatment Dimension 0.164% (10-3-10) was combined with TGR 0.34% (15-0-29) on May 1, 2001 and TGR was applied alone eight weeks later on June 28, 2001. All granular materials were applied using a 'shaker dispenser1to ensure uniform coverage. Turf quality was monitored from May 10 through September 5 (Table 1). Quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable and 1 = worst quality. Poa annua control was recorded from May 10 through September 5 (Table 2). Poa annua populations were determined by estimating the percentage area per plot covered by Poa annua. In addition, differences in the percentage of Poa annua seedhead production per plot were noted for May 15 and July 24 (Table 3). Data were analyzed using the Statistical Analysis System (SAS Institute Inc., 1989-1996) and the Analysis of Variance (ANOVA) procedure. Treatment effects on Poa annua populations and visual quality were tested using Fisher's Least Significant Difference (LSD) means comparison test. In addition, Poa annua percentage cover means were compared using orthogonal contrasts in the General Linear Model (GLM) procedure (Table 4). There was significant improvement in treated bentgrass quality on May 10, July 3, August 10, and August 15 as compared with the untreated control (Table 1). On other dates, numerical increases in quality ratings were noted but these were not statistically different. Treatment with TGR produced a definite color response in the bentgrass but not the Poa annua. The dark green bentgrass was in stark contrast to the lime green of the Poa annua and produced 'mottled or spotted' plots as compared to the untreated control and bentgrass treated with Turf Enhancer. Quality ratings on June 6 and June 15 reflect the distinct differences among the treatments but they are not statistically significant. Populations of Poa annua were reduced in treated bentgrass from June 1 through July 3 as compared with the untreated control but the reductions were not statistically significant on all dates (Table 2). After July 3, Poa annua die-back resulted in greatly reduced populations in treated and untreated bentgrass and treatment effects are not significant. Percentage Poa annua seedhead production data were taken when there was a large amount of seedhead formation on the practice green. On May 15, there were no statistical differences among the treated and untreated bentgrass (Table 3). On July 24, there were more seedheads in bentgrass treated with Dimension + Turf Enhancer than in bentgrass treated with Dimension + TGR or untreated. The orthogonal contrasts show that on some collection dates Poa annua populations in treated bentgrass plots were significantly different when compared individually with the untreated bentgrass or the other treatment (Table 4). 45 or-; oo oo e os Visual quality1 o f creeping bentgrass on a sand based green treated for the 2001 Dimension + TGR/Turf Enhancer Safety Study. 3 04 © K cn ro SO 0 4 K 00 oo <75 o- r- o 00 00 < ^ o 3 ^ < " p oo p Os r^ 0 0 00 00 o o o Os Os Os p fO 0 0 00 o o 04 o o o oo 60 Os ro o- o Os < ~ o o 04 Cd p rn §>© oo S' cn ^ f .0 "g p2 *52 m o o <* £ O Q o£ zr ^ cd V» 0s- ^2 cd *6 B £ Cd B VO *.5 15 Q o '£ J C no ~ rn g 'S -O 03 H Oh QJ VO oo 04 00 § w ■§ § o Os 04 .2 00 g ■ cd T1 comparing percentage Poa annua cower in turf treated for the 2001 Anderson's Dimension/TGR/Turf Enhancer Safety Study. Material Dimension + Turf Enhancer vs untreated control Dimension + Turf Enhancer vs Dimension + TGR Dimension + TGR vs untreated control Material May 15 May 23 June 1 June 6 June 15 June 21 June 29 July 3 0.9056 0.6213 0.0213 0.0535 0.0872 0.0036 0.0241 0.2746 0.3191 0.0557 0.2879 0.3099 0.2475 0.0705 0.3892 0.5614 0.2751 0.0993 0.0080 0.0179 0.4181 0.0213 0.0618 0.5614 Aug 31 Sept 5 July 11 July 24 Aug 2 Aug 10 Aug 15 Dimension + Turf Enhancer 0.8185 0.1401 0.0816 0.6433 0.4818 1.000 0.5823 vs untreated control Dimension + Turf Enhancer 0.5032 0.0816 0.2005 0.1161 0.0808 0.4818 0.6575 vs Dimension + TGR 0.0668 0.4818 1.0000 0.1963 0.9106 Dimension + TGR 0.6499 0.7747 vs untreated control 1These figures represent the probabilities that a greater value for T would occur by chance alone and were produced using the General Linear Model procedure and orthogonal contrasts. Turf enhancer (14-0-28) was applied initially with Dimension (10-3-10) on May 1 and then by itself on June 8 and June 28, 2001. TGR (15-0-29) was applied initially with Dimension (10-3-10) on May 1 and by itself eight weeks later on June 28, 2001. 48 Field Evaluation of Roundup Ready® Creeping Bentgrass Shui-zhang Fei and Rodney A. St. John Roundup Ready creeping bentgrass offers great promise for the golf industry because it will allow for more selective, effective and thus, simplified control of aggressive annual and perennial weeds in golf course turf. Weeds that can be easily controlled with Roundup® brand herbicides include annual bluegrass, roughstalk bluegrass, bermudagrass and many other grassy and broadleaf weeds. A greenhouse study of Roundup Ready creeping bentgrass at Iowa State University has demonstrated that key morphological data and pollen longevity of Roundup Ready creeping bentgrass are essentially the same as its conventional counterpart except the roundup ready phenotype. The purpose of this research was to evaluate the performance of Roundup Ready creeping bentgrass under field conditions. This research was established on September 13, 2001 with three replications of 5x5 ft (25 ft2) plots for each cultivar. A 2 feet border area between plots was seeded with perennial ryegrass. The experimental design was a randomized complete block with split plot. Seven conventional cultivars of Crenshaw, Penncross, Penneagle, Providence, Backspin, A4 and L93 were used along with two leading Roundup Ready creeping bentgrass lines ASR 333 and ASR368. The trial was fertilized with 1 lb N/1000 ft2 and 1 lb P/1000 ft2 during the establishment. Three weeks after seedling emergence, half of each plot received roundup application at a rate of 32 oz /acre. Data on turf quality, weed development and other morphological characteristics after the roundup application are not available at this time. 49 Corsair Perennial Ryegrass Control Study B.R. Bingaman, T. R. O ster, N. E. C hristians The objective of this study was to screen Corsair for removal of perennial ryegrass from a ryegrass/creeping bentgrass area maintained at fairway height. This study was conducted at Veenker Golf Course in Ames, IA on the border area between a bentgrass fairway and the adjacent ryegrass/Kentucky bluegrass rough. The bluegrass rough contained approximately 50% ryegrass. The experimental design was a randomized complete block and three replications were conducted. Individual plot size was 5 x 5 ft. The plots were arranged so one half of the plot was covered by bentgrass and the other by bluegrass/ryegrass turf. Corsair was applied at 1, 2, and 3 oz/A on August 8 using a carbon dioxide backpack sprayer equipped with TeeJet #8006 nozzles at a spray pressure of 30-40 psi. The herbicide was mixed with water to a volume equivalent to 3 gal/1000 ft2. Ryegrass damage was recorded on August 10, August 16 and August 23. Damage was assessed using a 9 to 1 scale with 9 = no damage, 5 = 50% ryegrass mortality, and 1 = 100% ryegrass mortality (Table 1). Phytotoxicity on bentgrass was rated using a 9 to 1 scale with 9 = no damage, 5 = 50% bentgrass damaged, and 1 = 100% bentgrass damaged. Bentgrass damage data were taken from August 10 through October 19 (Table 2). Kentucky bluegrass quality was monitored for possible phytotoxicity from August 10 through October 19 (Table 3). Turf quality was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst quality. Ryegrass control was determined by estimating the percentage cover per individual plot (Table 4). These data were taken initially on August 23 to assess the amount of ryegrass killed. Subsequent cover data were taken to monitor if there was any regrowth. Percentage cover data were taken for bentgrass to reflect the amount of mortality in the treated plots (Table 5). These data were taken from August 10 through September 19. The percentage bluegrass/ryegrass cover also was monitored from August 10 through October 19 (Table 6). These figures reflect the spread of bluegrass throughout the areas devoid of ryegrass in the treated plots. Data were analyzed using the Statistical Analysis System (SAS Institute Inc., 1989-1996) and the Analysis o f Variance (ANOVA) procedure. Treatment effects on turf populations and turf quality were examined using Fisher's Least Significant Difference (LSD) test. There was no damage on ryegrass until approximately 12 days post treatment (Table 1). Yellowing was beginning on August 20 and by August 23, the treated ryegrass was 'brown' and dead. The bluegrass/ryegrass areas of each treated plot were monitored through October 19 and there was no regrowth of ryegrass (Table 4). There were no phytotoxic symptoms on the treated bluegrass (Table 3). Bluegrass spread into the bare areas of the treated plots and by September 28, plots treated with Corsair at 1.0 and 2.0 oz/A were similar to the untreated controls in turfgrass percentage cover (Table 6). On August 23, significant levels of damage were observed on the treated bentgrass (Table 2). Damage was not significant on bentgrass treated with Corsair at 1 oz/A on August 23 and August 30 as compared with the untreated controls. However, bentgrass treated at 2.0 and 3.0 oz/A exhibited significant levels of damage as compared with the untreated controls on August 23 through September 6. As much as 50% of the bentgrass treated with Corsair at 3.0 oz/A was damaged and suffered severe browning and some mortality. By September 21, the effects of Corsair on percentage cover and bentgrass quality were no longer recorded (Tables 2 and 5). Bentgrass had moved into the bare areas of the treated plots and some of the damaged bentgrass had regrown from the crowns. This study will be monitored through the spring and summer o f 2002 for possible regrowth of perennial ryegrass. 50 cd On Vi ; ON CN oo oo oo © 0> 2 ON VO vo so Z O CO CO CO c/3 p © © p x /i ON ON ON ON Z p p p p 00 O n On O n on o Z O O O O C/5 p do p OS —' ^ —* Z p < p p p 00 On On On On Z ’O y £ ~ « CM (L d> o p Damage on perennial ryegrass1 treated for the 2001 Riverdale Corsair Ryegrass Control Study. o p OO On On On On Z 00 P < Table 1. p £ OO §) d> -O < Z) C /3 £ o o p o p p w On On On ON Z & •o' 5 00 G o U 6£ < » H, on p p P d> C* CZ) ON ON On On Z P 00 co p & £ o U d> o < o o o Z ^ ' ri r j o oN C §13 -C S p )^2 < & 00 c Cd ^ oo o - ^ co o P * dfi d>b d a m dO db p d> dfl *£ B M= < P ^ P P O O &0 O n ON ON ON Z o © d> p O n p O n p O 00 ON ON Z £ Id c 2 £ 05 o *5 3 < Od o < © © © Z ^ O p O O CO On ON ON On Z & £ O co p p p 00 co © © © in 3. < m CO 3 Ta K O < © © © Z (N n in II in oo & Rate -5 o < © © © z £ ’o ^S •S' 2 © O 00 o o O OS © o 2 © © p r- p c n so 00 OS 00 © © © © © © x © vs vs vs 00 00 X CN r - © vs r - © o c O U P © © r- r- c^- go © so SO so Z O oo 00 h «-H a> X £ 00 2 CN Oh o c/a u X) e 2 U September a ^3 X i s o0> p so OS 1- H GO P © © r-' © p OS © SO v s v s OS OS 2 Vi 2 Dh CO 6 UO X § X August O h X Material Vi X cd H O c cd cd •S cd o d § fS C e q o o O GO U U U nJ ■S 8 « 2 S ii — e ^ 3 S' < o'co co CO 'G < o o o o o o o in Z w n rn fn rn n w -' cO : tfl C CO O no C* ox _ & ^ o _• fcd 00 < p p p p p p p p p OVOVOsO^OsOVOVOVOV 00 p p p p p p p p p < ™ OSOsOVOSOSOSOVOsOS 00 ^ p p p p p p p p p OSOSCsOvasOSOsOsOS Table 2. Turf quality of Kentucky bluegrass treated for the 2001 Aventis Preemergence Annual Grass Study (July 11 - August 28). 2 scr VO 60 p p p p p p p p p < ^ 00 00 00 00 00 00 00 00 OO 00 p o o p p o p o o 00 OO 00 OO OO 00 00 00 00 3 CN tE : p p p p p p p o o p p p p p p p o o VO ir> p p p p p p p p p 3 3 ^ -D s o oX £ £ OS O p s i . o — o «* ^ SO ^ o 13 (N CN c © « rM bOsn vo 8 N l o ■ C<*u os sn as so ^^ T TiO t J Z Zfrh ZP3 GO GO a a oo oc < < &o & H H o (Nm^tunvor^ocas a 5 i-iSl g < D s £?00 M < C N t vq N t 00 oo 00 os O p C M * cn < N Tf cn p rn p O cn p p O p r- o »n in ri Tt rj \o ffi q q q q t ^ q q q ^ iri d (N -- m" >— 3 — 03 (N in (N o o o o o o o o o • x> o o o o o o o o o O o o o o o o o o rm 1 £ — > cd (N o c O F m | i " rt p c 5 w ts * o< ~ -a 2 is § « T o a) ex, § cs \= ox PL_, in a *2 3 o 3V i .s a C . o s o « cd c3- Q so S O ‘ ©O N£ NN t: ^ 0OX O 97% for July 11 through September 13. The predominate weed species covering the previously treated plots was crabgrass. Percentage crabgrass cover was similar in treated plots but was significantly higher than in untreated plots (Table 3). There were no differences in dandelion numbers on September 13 among the treated and untreated plots (Table 4). There was no clover in the previously treated plots on September 13. Second application: When larger weeds (7-9") were treated, similar levels of plant death at four days post treatment were produced for the herbicides. Total 'kill' of all plant material in these plots was recorded on August 9. Live weeds were first noted in the treated plots on August 21 and by September 13, there were similar levels of weed cover in the treated plots (Table 2). Percentage weed cover was 26.7% for ZPP1560 and 20.0% for Roundup. Crabgrass and dandelion populations also were similar in the treated plots on September 13 (Tables 3 and 4). 59 Table 1. Percentage dead area1 in turfgrass treated for the 2001 Syngenta Weed Control Study. Material 1. 2. 3. 4. 5. Untreated control ZPP1560 Roundup 0.96% a.i. ZPP1560 Roundup 0.96% a.i. Weed height at application NA 3-5" 3-5" 7-9" 7-9" 0.0 41.7 75.0 0.0 0.0 15.5 LSDo.os Material 1. 2. 3. 4. 5. May 22 Untreated control ZPP1560 Roundup 0.96% a.i. ZPP1560 Roundup 0.96% a.i. Weed height at application NA 3-5" 3-5" 7-9" 7-9" July 30 0.0 0.0 0.0 85.0 80.0 May 29 June 8 June 15 0.0 100.0 100.0 0.0 0.0 0.0 100.0 100.0 0.0 0.0 0.0 100.0 100.0 0.0 0.0 — — — Aug 9 Aug 16 Aug 21 0.0 0.0 0.0 100.0 100.0 0.0 0.0 0.0 100.0 100.0 0.0 0.0 0.0 100.0 100.0 June 21 0.0 60.0 71.7 0.0 0.0 11.4 Aug 28 0.0 0.0 13.3 90.0 91.3 — — — 13.8 20.7 LSDo.os 1These data represent the percentage area in each plot covered by dead plants. *Ready to use formulation applied at 100 gallons/A to provide 'too wet' coverage. First applications (3-5" weed height) were made on May 18 and second applications (7-9" weed height) on July 27, 2001 NS = means are not significantly different at the 0.05 level. 60 July 11 0.0 0.0 0.0 0.0 0.0 July 17 1.7 1.3 2.7 0.0 0.0 — NS Sept 13 Mean 0.0 0.0 0.0 66.7 80.0 0.0 31.0 35.6 41.8 42.7 20.2 2.2 Table 2. Percentage weed cover in turfgrass treated for the 2001 Syngenta Weed Control Study. Material 1. 2. 3. 4. 5. Untreated control ZPP1560 Roundup 0.96% a.i. ZPP1560 Roundup 0.96% a.i. Weed height at application June 28 NA 3-5" 3-5" 7-9" 7-9" 30.0 70.0 66.7 50.0 28.3 34.0 LSDq.06 July 3 July 11 July 17 July 25 Aug 9 33.3 70.0 66.7 51.7 28.3 36.7 100.0 100.0 31.7 28.3 38.3 98.7 97.3 38.3 35.7 61.7 100.0 100.0 60.0 63.3 61.7 100.0 , 100.0 0.0 0.0 32.6 19.3 23.6 24.2 10.6 Aug 28 Sept 13 Mean 50.0 100.0 100.0 10.0 8.7 53.3 100.0 100.0 26.7 20.0 48.8 93.9 93.1 26.9 21.4 6.4 10.6 10.6 12.9 L S D 0.05 ‘These data represent the percentage area in each plot covered by green, healthy weeds. *Ready to use formulation applied at 100 gallons/A to provide 'too wet' coverage. First applications (3-5" weed height) were made on May 18 and second applications (7-9" weed height) on July 27, 2001 9.9 Material 1. 2. 3. 4. 5. Untreated control ZPP1560 Roundup 0.96% a.i. ZPP1560 Roundup 0.96% a.i. Weed height at application Aug 16 NA 3-5" 3-5" 7-9" 7-9" 61.7 100.0 100.0 0.0 0.0 61 Aug 21 61.7 100.0 100.0 1.0 1.0 Table 3. Percentage crabgrass cover1 in turfgrass treated for the 2001 Syngenta Weed Control Study. Material Weed height at application June 28 NA 3-5" 3-5" 7-9" 7-9" 28.3 65.0 63.3 26.7 25.0 July 3 July 25 16.7 85.0 85.0 5.0 3.7 25.8 79.6 78.3 25.8 21.3 20.3 33.2 11.4 17.1 LSD 0.05 These data represent the percentage area in each plot covered by crabgrass. *Ready to use formulation applied at 100 gallons/A to provide 'too wet' coverage. First applications (3-5" weed height) were made on May 18 and second applications (7-9" weed height) on July 27, 2001 15.0 1. 2. 3. 4. 5. Untreated control ZPP1560 Roundup 0.96% a.i. ZPP1560 Roundup 0.96% a.i. Table 4. 25.0 98.3 98.3 43.3 31.7 Percentage dandelion and clover cover on September 13 in turfgrass treated for the 2001, Syngenta Weed Control Study. Material 1. 2. 3. 4. 5. 33.3 70.0 66.7 28.3 25.0 Mean Sept 13 Untreated control ZPP1560 Roundup 0.96% a.i. ZPP1560 Roundup 0.96% a.i. Weed height at application Dandelion NA 3-5" 3-5" 7-9" 7-9" 15.0 16.7 15.0 13.3 11.7 Clover 16.7 0.0 0.0 0.0 1.7 NS 6.3 LSDo.os 1These data represent the percentage area in each plot covered by either dandelion or clover. *Ready to use formulation applied at 100 gallons/A to provide 'too wet' coverage. First applications (3-5" weed height) were made on May 18 and second applications (7-9" weed height) on July 27, 2001. NS = means are not significantly different at the 0.05 level. 62 Monsanto 78365 Demonstration for Summer Fastburn Symptomology in Turfgrass B.R. Bingaman and N. E. Christians This study was designed to demonstrate at the 2001 Turf Field Day the fastburn symptomology capability of MON 78265 at one, two, three and seven days post treatment and to compare this symptomology to that of Roundup Pro Dry. This study was conducted at the Horticulture Research Station north of Ames, IA. The plot was in established area of premium Kentucky bluegrass sod mix. The soil was a Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll) with 4.1% organic matter, 93 ppm K, 11 ppm P, and a pH of 6.75. The experimental design was a randomized complete block. Three replications were conducted and individual plot size was 5 x 5 ft. Experimental MON 78365, Roundup Pro Dry, and Roundup Pro Dry + Scythe 1EC were applied at various intervals before ISU Turfgrass Field Day on August 2 (Table 1). The 14 day before field day treatments were made on July 19, 7 days before on July 26, 3 days before on July 30, 2 days before on July 31, and 1 day before on August 1. All materials were applied using a carbon dioxide backpack sprayer equipped with TeeJet #8006 nozzles at a spray pressure of 30-40 psi. Liquid formulations were mixed with water to a volume equivalent to 3 gal/1000 ft2. Efficacy of the formulations was assessed by estimating the amount of'brown' (dead and dying) plant material per plot. These percentage data were taken from July 24 through September 26 (Table 1). The Statistical Analysis System (SAS Institute Inc., 19891996) and the Analysis of Variance (ANOVA) procedure were used to analyze the data. Treatment effects were examined with Fisher's Least Significant Difference (LSD) means comparison test. By July 30, turf areas treated with Roundup Pro Dry at 14 days before field day were totally brown and turf treated with MON 78365 on July 26 was 81.7% brown (Table 2). Turf treated with Roundup Pro Dry alone or Roundup Pro Dry plus Scythe on July 26 had 76.7 and 56.7% brown area, respectively. There were significant differences in brown areas among the treatments on August 3, one day after field day. Dead areas were similar for the treatments applied seven days before field day. The three days before field day treatments also were similar with 78.3% brown area for MON 78365 and 68.3% for Roundup Pro Dry plus Scythe. The symptomology on turf treated two days before field day showed distinct differences. Treatment with MON 78365 resulted in 76.7% brown area and Roundup Pro Dry plus Scythe produced 61.7% brown-out. However, turf treated with Roundup Pro Dry alone had only 16.7% brown area. Percentage brown areas in turf treated with the one day before field day applications were 66.7 and 43.3% for MON 78365 and Roundup Pro Dry plus Scythe, respectively. From August 9 through August 28, there was 100% brown-out in all treated turf. Regrowth of plant material was noted on September 13 but by September 26, brown areas in treated turf were still greater than 91% for all materials. 63 Table 1. Timing and rates of application for nonselective herbicides used in the 2001 Monsanto 78365 Demonstration Study. Timing of application (days prior to Field Day- Aug 2) 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Material Rate a.i./A 14 days July 19 Untreated control MON 78365 66.6% MON 78365 66.6% MON 78365 66.6% MON 78365 66.6% Roundup Pro Dry 64.9% Roundup Pro Dry 64.9% Roundup Pro Dry 64.9% Roundup Pro Dry 64.9% + Scythe 1EC Roundup Pro Dry 64.9% + Scythe 1EC Roundup Pro Dry 64.9% + Scythe 1EC Roundup Pro Dry 64.9% + Scythe 1EC NA 61b 61b 61b 61b 61b 61b 61b 61b 12 qts 61b 12 qts 61b 12 qts 61b 12 qts NA 7 days July 26 3 days July 30 2 days July 31 NA NA NA 1 day Aug 1 . NA XXXXX XXXXX XXXXX XXXXX xx x x x xxxxx xxxxx xxxxx xxxxx xxxxx XXXXX 64 O co o § £ © CO in CN CO CN o Os Tf Tf OS os so OS OS Os OS O © OhO o o co o o CO o o o co © Os OS os os OS OS OhCO CD ?S SP cs. 5 < <, S T—* OX) p ns < OX) 3 co < J^T* c o n g 'r t 5 r-o s OS OS Os s o Os s o OS OS OS OS Os OS OS Os o co © co co r - O ros in Os Os OS OS s o OS OS OS OS OS OS OS Os « vo -H f- Q ro rr- o i> Os OS Os o s OS OS o s o s o s Os OS o o o o o o o o W) 3 Percentage dead area1 in turfgrass treated for the 2001 Monsanto 78365 Demonstration Study. CO p in in os OhVO CUD CN r< /L \> VfNl N C/J Table 2. , co in 00 Os VO in 0 0 0 0 0 0 s o OS OS Os OS Os OS OS oo OS © ,—i o co CO 00 00 o OS o d o d r** t SO s o r - so o co o co s o o OS o d o 00 i i i i i i i i 1 1 1 1 1 1 1 1 i i i i • i CO 1 1 CO 1 r - _ ^tj- < Z r - CO CO OS 0 0 0 0 OS OS OS f - CO so oo 00 so so CO Tf 1 1 1 1 1 1 1 1 ■ 1 1 1 1 1 1 1 1 1 1 1 1 J in vo r— so r - i 1 1 1 1 1 so -! o U ng PQ so SO so so O h Oh CU Oh ~ £ £ s Oh tz: CO co CO co O U Oh 00 oo oo 00 Oh Oh P. O h 3 T3 3 .C 3 i f -C hO r- r» r- r- T3 X) T3 t- T3 Z Z Z Z C3 C3 cos c3 c3 o § *Q• §S o o o o O O O O ^ O c/> g ^ g £ s £ C* 0^ £* Cd + 04 + C* + Ofii N ° N® ox ox *C N ® SO Q \ ON o \ os as OS os N O Tf SOSOSOSO ox CN CO K OO 0\ >? CD Broadleaf Herbicide Study “Lontrel & Confront” F. Valverde andD .D . M inner Introduction Weeds cause problems is general appearance and visual quality of lawns. A single weed in a lawn may be considered a problem for the intended use of that area. Thus it becomes obvious that any method that helps to eliminate or decrease the population of weeds is a point of interest and it requires attention. Due to the perennial crop cycle in most turf grasses, herbicides that are selective to different weed species are necessary. Lontrel and Confront are selective herbicides in turf species aimed to control several broad leave species. The objective of this study is to test the effectiveness of these two products controlling some broadleaves and to observe their effects on the crop itself. Materials and Methods This herbicide study was conducted on a mature stand of common Kentucky bluegrass at the Horticulture farm of Iowa State University from May to August of 2001. The site was located 6 miles north of Ames, Iowa. Two different herbicides, Confront and Lontrel at two different concentrations were applied over Kentucky Bluegrass (Poa pratensij) plots. The study followed a complete randomized block design with 5 treatments including control and 4 replications. Each replication had 5 plots of 5x5 ft. separated by a 2 ft. border between them. Replications were separated by 2 ft. The area had a very uniform and dense population of White Clover (Trifolium repens) and Dandelion (Taraxacum officinale). Other weeds were present in the surrounding area but in a very limited number and not evenly distributed. Black Medic (M edicago lupulina) also appeared inside the study area but in such scarce level that no statistical analysis was performed; however data is shown at the end. Treatments were applied using a backpack CO2 sprayer on May 15. Lontrel was applied at 0.5 and 1.0 PT PR/A, Confront was applied at 1.0 and 2.0 PT PR/A. Control plots only received a water spray. Visual assessment of turf and weeds was conducted at 2, 4, 6 and 10 weeks after treatment application. The variables recorded were % cover of each of the present weed species and also the turf crop. An injury index was established and recorded for the same species. This index ranged from 0 (no injury) to 10 (dead but still present). Percent weed control was calculated using the percent cover of each of the weeds in each treatment-plot compared to the controlplot in its respective replication at the time of evaluation. Analysis of variance and LSD were calculated for each the recorded variables. Raw data and statistical tests appear in the appendix tables at the end of this report. Results Crop Injury Kentucky bluegrass in the study area remained actively growing during the entire study with no evidence of turf injury by treatments or environmental conditions. White Clover The percent control of White Clover appears in table 1. Both Lontrel and Confront provided good control of white clover. Two weeks after the application of herbicides there was already 50-70% reduction on weed populations. Their effects increased during the 4 and 6 week after application surpassing the 90% of control. By the 10th week new plants of white clover appeared although in very limited numbers. There was a significant difference between control and herbicides along the duration of the trial. But there were only significant differences between herbicides on the last two readings for Lontrel 0.5 compared to the other treatments. Lontrel 0.5 showed a lower degree of control. 66 Table 1. Percent of control (*) on White Clover (Trifolium repens) and Dandelion (Taraxacum officinale') observed in Kentucky Bluegrass (Poapratensià) plots on 4 different dates after herbicide application. Number of weeks after application 4 2 6 4 6 10 Dandelion % Control White Clover % Control 10 Treatments 2 Lontrel 0.5 PT PR/A 72.0 93.3 88.8 80.0 40.0 56.7 75.0 -20.0 Lontrel 1.0 PT PR/A 54.0 96.9 94.2 91.7 37.5 74.5 94.4 100.0 93.6 6.3 65.6 85.0 72.5 12.5 84.4 95.7 90.0 Confront 1.0 PT PR/A 57.1 95.0 95.6 Confront 2.0 PT PR/A 62.5 97.8 96.9 87.9 Control 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0001 0.0000 0.0000 Prob 0.0033 0.0000 0.0000 0.0000 0.2302 LSD 0.05 C.V. % 32.39 5.40 4.16 8.48 44.31 22.04 5.81 23.83 33.40 6.76 1.20 7.13 9.57 10.23 0.80 1.74 (*) Percent control based on percentage of species in treated plots vs. control plots. Dandelion In contrast as shown in table 1, the effect of treatments on Dandelion were evident only after the 4th week. Even though there are some evidence of effect by the 2nd week, this was not significantly different from the control as indicated by the anova. Four weeks after application herbicides showed between 55 and 85 % of control compared to the non-treated plots and they were significantly different against control but not among them. The effect of herbicides are more clear by the 6th week where they not only show significant differences against control but also between chemicals and their rates of application. At the lowest rate applied Confront gave better control of dandelion than Lontrel. At the highest rate Confront and Lontrel provided similar dandelion control and both resulted in about 95% of the control. By the last reading date, Lontrel 0.5 was not significantly different than the control, however Lontrel 1.0 showed a perfect (100%) control of dandelion. Confront at both rates showed a very similar behavior as the previous reading date. However, there were already indications of new plants of dandelion growing at this time. Total W eed Control As it can be observed in table 2, weed populations were dense at the starting point. More than 50% of the plots were covered with weeds at the onset of the trial. Four weeks after treatment application on control plots, in average weed populations had over 80% of the area covered. This makes even more dramatic the observed effect of herbicides. The analysis of variance showed significant differences between treated and non treated plots at all four reading dates, being the difference much clearer at 6 weeks after treatment application. Table 2. Percent of total weed cover observed in Kentucky Bluegrass (Poapratensis) plots on 4 different dates after herbicide application. Weeds % Coverage 2 weeks after applic. 4 weeks after applic. 6 weeks after applic. 10 weeks after applic. Lontrel 0.5 PT PR/A 22.0 15.0 7.3 10.0 Lontrel 1.0 PT PR/A 30.3 14.0 4.3 4.3 Confront 1.0 PT PR/A 33.8 16.3 6.3 9.8 Confront 2.0 PT PR/A 28.8 7.3 2.8 6.8 Control 52.5 81.3 65.0 57.0 Prob. 0.0030 0.0000 0.0000 0.0000 LSD 0.05 C.V. % 12.97 12.04 11.19 15.24 5.63 6.53 9.50 12.60 67 There were significant differences between herbicides-rates during the 2 first readings but not at 6 or 10 weeks after treatment application. In the first reading Lontrel 0.5 showed the best control with only a 22% cover of weeds in average. Four weeks after treatment Confront 2.0 is significantly better than the rest of treatments with only 7% of weed cover. Although there are no significant differences among herbicides 6 weeks after application, Confront 2.0 showed the minimum percent cover of weeds during the whole trial. Control plots also showed a decrease in the percent cover of weeds after the 2 reading, maybe as a product of the dry conditions that were present during most of June and July. Conclusions Two major points can be extracted from this trial. First is the effective control of dandelion and white clover by Lontrel and Confront without any injury to Kentucky bluegrass. Second, although not statistally significant, the high rate of Confront gave the greatest level of weed control Also it was observed that white clover was affected by herbicides some time sooner than dandelion. Dandelion was harder to eliminate and new plants were coming back at week 10. A second application later during the summer season may be considered. 68 Fine Tuning Calcium Chloride - Urea Ratios for De-Icer Purposes F.J. Valverde; D.D. M inner Introduction In areas where winters are characterized by the large amount of snowfall, it is a common practice to use “de-icers” as a mean to clear pathways, sidewalks and streets. The nature and characteristics of these products vary widely. However it is a common effect among all of them the damage they cause to turf areas due to the increased salinity in the surroundings of the treated area. Previous trials have defined a mixture of calcium chloride and urea as one of the most effective de-icers with minimum damage to turf species. Nevertheless this does not mean that areas treated with this product are exempt of injury. It has brought to attention that varying the ratio between urea and calcium chloride could possibly decrease the negative effect on turf without decreasing considerably the melting capabilities of the de-icer. The objective of this study is to compare the effect that different mixtures of calcium chloride and urea applied on winter can have on turf quality and appearance during spring time. Materials and Methods This de-icer study was conducted at the Horticulture farm of Iowa State University from December 2000 until June 2001. The site was located 6 miles north of Ames, Iowa. Different mixtures of calcium chloride and urea were applied over Kentucky Bluegrass (Poa pratensfy plots. The study followed a complete randomized block design with 8 treatments (Table 1) including control and 3 replications. Each replication had 8 plots of 4x2 ft. separated by a 1 ft. border between them. Replications were separated by 2 ft. Each treatment was applied weekly during 9 weeks starting on December 15. To reach a concentration of 2.5% W/V (Brine solution), 2 liters of water was used in each application. This simulated a 1/10 inch water film over the grass. Treatments were initially applied using a backpack C 0 2 sprayer, however due to the extreme cold conditions a hand sprayer was used in the last 6 applications. Control plots only received a water spray. Also it must be noted that due to the particularity of the site and weather conditions it was required to remove the snow from the treated plots in the first 3 applications. The accumulation of snow at that point reached over 12 inches, which would not allow a uniform application of treatments. A snow blower and a brush were used for this purpose. Table 1. Total amount and ratio of calcium chloride and urea applied over 8 ft2 in each of the 9 applications. Treatment Urea CaCl2 % gr/plot % gr/plot 30 15.0 1 70 35.0 2 75 37.5 25 12.5 3 80 40.0 20 10.0 4 85 42.5 15 7.5 5 90 45.0 10 5.0 6 95 47.5 5 2.5 7 100 50.0 0 0 0 0 0 0 8 Treatments were evaluated on 4 different dates, March 26, April 14, May 9 and June 1. The variables recorded were color, percentage coverage of turf plants coverage and % coverage of turf plants with seed heads. The index of color was based on a scale of 1 to 10 where 1 refers to total brown or white color and 10 dark even green; 6 is considered the least acceptable green. The other 2 indexes are referred on a percentage basis. Analysis of variance and LSD were calculated for each of the recorded variables. In June 1 plant samples were collected for total nitrogen. Results of those test will be submitted at a later date of this report. 69 Results To better understand the results shown in this report, it is necessary to consider that the winter of 2000-2001 in the Ames area had over 100 days with permanent snow cover. The surrounding area where the treatments were applied maintained over 6 inches of snow since early December until late March. Temperatures were very low for a long period of time. The readings of turf color appear in table 2. As expected after a long cold winter, the first reading indicated that most of the plants were completely brown,. At this moment plots that received a larger amount of urea were significantly different to those with very little or none urea applied. Also it can be noted that control plots had statically better appearance than the treated ones. Near 3 weeks later in a second reading , the results have varied very little. Due to the still cold temperatures, no new growth was expected. Results are very similar to the previous date. Control plots are still statically with better color than the treated ones. Table 2. Observed color in CaCl2-Urea plots at 4 different dates during spring of 2001. CaCl2/Urea ratio March 26 April 14 May 9 June 1 70/30 1.00 1.00 3.00 9.67 75/25 1.67 1.00 2.33 8.67 80/20 1.33 1.67 2.67 8.33 85/15 2.00 1.67 2.00 7.00 90/10 2.00 7.33 1.67 1.33 95/5 2.00 2.00 2.33 6.00 100/0 2.00 1.67 2.00 4.00 Control 3.00 3.00 6.00 6.00 Prob. 0.0007 0.0013 0.0000 0.0001 LSD 0.05 0.648 0.752 0.926 1.6157 In May 9, during the third reading it can be appreciated that plants already started to grow and new green tissue is already present. Again there were significant differences between the treatment with the largest amount of urea and the one with 100% CaCl2, however the relationship is inverse to the 2 previous readings. In this case the treatment with the larger amount of urea show a better color than any other treatment (excluding control). Although the increment on the 70/30 treatment is higher enough to be the best treatment in this reading, the increment in the control plots are far better. Two major points can be extracted from this observation. The first is that the availability of extra nitrogen in the high urea treatments may be already stimulating new growth. However the damage inflicted by the de-icer is still greater than the benefit obtained at this point. In the last reading, after a considerable improvement in growing conditions, treatments with at least 20% of urea had significantly better color than the control. Treatments with 15,10 and 5 % of urea improved considerably from last reading however they were not significantly different than the control. Control plots did not show an improvement from previous readings, which may be attributed to low levels of nitrogen available in the soil. Treatments with no nitrogen added and high calcium chloride are statically with worse color than any other treatment. It is clear at this point that supplemental nitrogen in the de-icer mix has overcome the damage inflicted previously. Due to the lack of color development in the last period of time by the control plots it is believed that the area in which the trial was applied has a very limited source of nitrogen. Other areas with higher levels of nitrogen may not have shown differences between control and de-icer applications. Other variables recorded were percentage of coverage and percentage o f area cover with seed heads (table 3). In May 9, the percent coverage followed the same pattern observed in color for the same date. The tendency (excluding control) showed a better coverage in plots with higher ratio of urea; although there were no significant differences between treatments that contained urea. The only significant difference appears between the treatment with 100% CaCl2 and all the others. 70 Table 3. Percentage turf coverage and percentage of area cover with seed heads. CaCl2/Urea ratio % Turf Coverage May 9 % Coverage Seed heads June 1 28.33 70/30 96.67 75/25 91.67 11.67 80/20 90.00 18.33 85/15 91.67 16.67 90/10 85.00 10.00 95/5 86.67 13.33 100/0 60.00 10.00 Control 100.00 88.33 Prob. 0.0006 0.0000 LSD 0.05 13.26 14.32 Control plots showed a 100% coverage at this time, indicating again that even the safest mixture of CaCl2 and urea had a detrimental effect on the plants. A very interesting phenomenon occurred in the treated area and surroundings. Towards the end of May the turf started to produce seed heads in a very dense fashion. Some differential effect on the treatments was observed and this variable was recorded and shown in table 3. Before looking at the numbers it was believed that areas with low nitrogen could be the ones having the largest amount of seed heads due to a stressed condition. After looking at the analysis of variance this idea was not clear any more. Results indicate that the largest amount of seed heads appeared in control plots, however within treatments the one with higher ratio of urea showed the highest number of seed heads. It is still possible that the low available nitrogen has triggered the higher amount of seed heads in the control plots. In the case of de-icer treatments differences may be due to the differential damage observed in previous dates and not directly to nitrogen availability. This point will be cleared when results from total nitrogen come from the laboratory. As an extra note, during the first readings, it was appreciated that even the control plots of the study were in a worse state of color and quality that the area next to them, where no snow was removed. Its is believed that the long and cold winter affected extensively the treated area. The layer of snow that remained around the study could have protected the turf, therefore its greener color and denser condition. Conclusions There are significant differences even between small variations in the ratios of urea and CaCl2. Under the conditions of this study the mixture of 30% urea and 70% CaCl2 seems to be the least damaging of the options. Also this ratio seems to be the one with higher benefits and better color development. Benefits if there are, are not expected to be evident until late spring. 71 Brown Patch Fungicide Trial M ark L .Gleason, Stephen N .Wegulo, SaraJ. Helland, and John P. Newton Trials were conducted at Veenker Golf Course on the campus of Iowa State University. Fungicides, selected for activity against brown patch, were applied to creeping bentgrass maintained at 0.16-inch cutting height, using a modified bicycle sprayer at 30 psi and a dilution rate of 5 gal per 1,000 sq ft. The experimental design was a randomized complete block with four replications. All plots measured 4 ft x 5 ft. Fungicide treatments were all applied on 21 Jun, then re-applied at recommended intervals on 2, 5, 12, and 20 Jul. Brown patch symptoms were first observed on 27 Jun. Most products suppressed brown patch and dollar spot significantly in comparison to the unsprayed control on 6 Aug. No phytotoxicity symptoms were observed during the trial. Fungicide products, application rates and intervals, and brown patch and dollar spot severity at the Veenker Golf Course, 2001 Product and rate per 1,000 sq ft Interval (days) Unsprayed check................................. — Eagle G (XF-00023) 4.0z.................... 14 Eagle G (XF-00023) 8.0z.................... 28 Eagle G (XF-00024) 2.5z................... . 14 Eagle G (XF-00024) 5.0z.................... 28 Eagle/Daconil (XF-00044) 3.5 oz...... 14 Eagle/Daconil (XF-00044) 7.0 oz...... 28 Golden Eagle 4.0 oz........................... 14 Golden Eagle 4.0 oz........................... 28 14 Banner MAXX 2.0 oz Bayleton 0.5G 1.5 oz.......................... 14 Daconil 82.5 WDG 3.2 oz................. . 14 Confidential 2.0 fl oz.......................... . 7 Confidential 4.0 fl oz......................... . 7 Confidential 2.0 fl oz......................... . 7 Confidential 4.0 fl oz......................... . 7 Confidential 2.0 fl oz.......................... . 7 Confidential 4.0 fl oz......................... . 7 Confidential 2.0 fl oz......................... . 7 Confidential 4.0 fl oz.......................... ..7 Confidential 2.0 fl oz......................... . 7 Confidential 4.0 fl oz......................... ..7 Daconil Ultrex 82.5 WDG 3.2 oz..... .14 +Primo MAXX 1 MEC 0.15 fl oz Daconil Ultrex 82.5 WDG 3.2 oz..... ,. 14 +Banner MAXX 1.3 MEC 0.5 fl oz +Primo MAXX 1 MEC 0.15 fl oz Heritage 50WG 0.2 oz....................... ..14 +Banner MAXX 1.3 MEC 0.5 fl oz +Primo MAXX 1 MEC 0.15 fl oz Banner MAXX 1.3 MEC l.O flo z ... ..14 +Primo MAXX 1 MEC 0.2 fl oz Heritage 50WG 0.2 oz....................... . 14 ^Banner MAXX 1.3 MEC 0.5 fl oz Brown Patch* 6/27/01 Brown Patch 7/11/01 Brown patch 7/24/01 Brown patch 8/6/01 Dollar Spot** 6/27/01 Dollar spot 7/11/01 Dollar spot 7/24/01 Dollar spot 8/6/01 1.3 1.0 2.3 0.3 0.5 0.5 0.5 1.5 1.3 1.0 1.0 1.0 2.3 2.0 1.3 0.5 1.5 1.8 1.3 1.3 1.3 2.8 1.0 3.5 2.3 1.5 1.0 1.8 3.3 2.0 1.3 1.5 0.0 0.0 0.0 0.3 2.0 2.3 2.3 1.8 1.3 2.0 3.3 2.3 1.3 0.3 3.8 3.8 2.5 2.3 2.5 3.3 1.5 2.3 2.5 0.0 0.0 0.5 0.3 4.5 2.3 2.3 1.3 2.5 1.5 2.0 2.5 2.5 0.0 3.8 0.8 2.5 1.3 0.5 3.5 0.5 1.5 2.3 0.0 0.5 1.0 0.3 2.0 1.5 0.5 0.3 0.8 0.5 0.3 1.5 2.0 1.0 3.3 12.5 4.0 8.0 7.4 11.5 10.3 10.0 2.5 3.4 8.9 4.5 15.0 4.8 4.8 0.8 19.3 9.5 3.5 8.8 8.8 10.8 10.0 10.3 20.1 6.0 15.1 14.0 20.5 16.3 10.5 7.5 0.4 1.2 2.3 7.5 8.5 11.3 3.1 25.8 10.5 11.0 13.0 17.5 17.5 2.3 9.3 14.0 7.4 14.0 9.9 21.5 5.1 11.9 8.1 1.7 0.0 0.8 1.3 9.0 11.5 1.5 17.0 11.0 10.8 12.0 10.5 16.5 0.3 16.5 1.0 5.5 9.8 5.0 20.0 10.1 4.8 9.6 0.0 0.0 1.8 4.5 9.0 0.0 4.0 1.8 23.8 11.3 13.8 13.8 4.5 2.3 0.8 0.0 0.0 0.0 1.6 0.1 0.4 0.0 0.5 0.0 0.0 0.0 3.8 0.6 0.1 0.3 1.3 0.0 0.3 0.0 6.1 1.3 0.3 0.0 0.0 0.0 0.0 0.0 4.3 0.8 0.2 0.5 2.4 MSD (K=100)*** 2.8 4.0 2.1 17.9 12.2 14.5 20.6 *Mean disease severity rating; 0= no disease, 1= 1-5%, 2= 6-10%, 3= 11-25%, 4= 26-50%, 5= >50% of plot symptomatic n= 4 **% of plot symptomatic. n= 4. ♦♦♦Minimum significant difference from the Waller-Duncan k-ratio test (K = 100). 72 Dollar Spot Fungicide Trial M ark L. Gleason, Stephen N Wegulo, S a ra J Heiland, and John P. Newton Trials were conducted at the Iowa State University Horticulture Station near Gilbert. Fungicides were applied to creeping bentgrass maintained at 0.16-inch cutting height, using a modified bicycle sprayer at 30 psi and a dilution rate of 5 gal per 1,000 sq ft. The experimental design was a randomized complete block with four replications. All plots measured 4 ft x 5 ft. The entire plot was inoculated with pathogen-infested rye grain on 8 Jun. Spray applications were initiated on 15 Jun in all subplots, then re-applied at recommended intervals on 5 and 13 Jul. The spray regime was suspended on 13 Jul because of absence of dollar spot symptoms. The entire plot was inoculated again on 14 Aug. All subplots were sprayed on this date and on 28 Aug. Dollar spot symptoms were first observed on 27 Jun. Most products suppressed dollar spot significantly in comparison to the unsprayed control on 27 Jun and on 23 and 31 Aug. No phytotoxicity symptoms were observed during the trial. Fungicide products, application rates and intervals, and dollar spot severity at the ISU Horticulture Station, 2001 Product and rate per 1,000 sq ft Unsprayed check Eagle G (XF-00023) 4.0 oz Eagle G (XF-00023) 8.0 oz Eagle G (XF-00024) 2.5 oz Eagle G (XF-00024) 5.0 oz Golden Eagle 4.0 oz Golden Eagle 8.0 oz Banner MAXX 2.0 oz Bayleton 0.5G 1.5 oz Daconil 82.5WDG 3.2 oz Chipco Triton 1.67SC 0.75 fl oz Chipco Triton 1.67SC 1.0 fl oz Chipco Triton 1.67SC 2.0 fl oz Severity (%) Interval (days) — 14 28 14 28 14 28 14 14 14 14 14 14 LSD(0.05) 73 6/27/01 1.88 0.25 0.58 0.38 0.53 0.10 0.65 1.00 0.43 0.58 0.40 0.90 0.18 8/23/01 5.50 3.63 2.80 2.38 2.25 2.75 2.13 0.43 0.43 1.03 1.25 0.40 0.35 8/31/01 5.88 7.75 4.75 5.75 6.50 4.88 3.80 0.15 0.05 0.50 0.65 0.23 0.08 1.19 2.03 5.40 Bermuda Species Traffic Study D.D. M inner andF.J. Valverde Introduction Seeded bermudagrass has been used in northern climates to repair worn athletic fields during the summer (Gaussion et al., 2001). The basic idea is to produce a fast crop of biomass on exposed-bare-soil areas of an athletic field. Football practice fields re­ established this way during the summer are superior to weedy and sparsely covered fields by the end of August or start of the fall football season . The bermuda may die in the winter, but there is a net gain of biomass cover. The following study continues these philosophy by evaluating bermudagrass varieties and mixtures with cool season grasses. Objective To evaluate seeded and sprigged bermudagrass varieties to repair intensively trafficked areas of northern athletic fields. To evaluate mixtures of cool and warm season grasses. M ethods This study was established at the Horticulture Research Farm in Ames Iowa on 11 July 2001. The trial combined 12 combinations of grass species and 2 levels of traffic, for a total of 24 treatments. The experimental design was a randomized complete block with split-plot arrangement. Whole plots consisted of grass species and split plots were traffic levels. There were 3 replications for a total of 72 sample units of 2 ft x 12 ft. The species or combinations of species and the establishment method used appear in table 1. Table 1. Description of species and cultivars and establishment method. Species B Planting time Treat. Species A Planting time 1 Yukon Jul-11 — — — — Establishment method Seed 2 Primo Jul-11 3 Primo Jul-11 Perennial Rye Jul-11 Seed 4 Primo Jul-11 Creeping Bentgrass Jul-11 Seed 5 Primo Jul-11 Kentucky Bluegrass Jul-11 Seed 6 Primo Jul-11 Perennial Rye Aug-21 7 Zoysia Jul-11 — —— 8 Westwood Jul-11 _______ __ ____ Sprigging 9 Quickstand Jul-11 — — Sprigging 10 Creeping Bentgrass Jul-11 — — Seed 11 Perennial Rye Jul-11 — — Seed 12 Kentucky Bluegrass Jul-11 — — Seed Seed Seed Seed Traffic treatments were applied with a GA-SWC traffic simulator (Cairow et al., 2001) on 11 September with 2 and 4 passes each Monday, Wednesday and Friday during the first month. From October 8 to November 1, traffic simulation was increased from 2/4 passes to 3/6 passes each day. The variables measured were % turf cover, turf color and turf quality. Data was collected monthly, from 23 July to 2 November 2001. Plant samples were collected on 4 November to measure biomass. 74 Results Table 2. Percent turf cover for various grass combinations and two levels of traffic. Traffic was applied from 11 Sepetember to 1 November 2001. Species Traffic Ley 7/23/2001 8/21/2001 9/11/2001 9/24/2001 10/8/2001 11/2/2001 ----------------------------------------- % cover -------------------------------------------Yukon Primo 23 85 88 97 98 90 27 100 100 100 100 100 Primo + PRl 50 98 100 100 100 98 Primo + CB 35 100 100 100 100 93 Primo + KB 22 100 100 100 100 100 Primo + PR2 22 100 100 100 100 97 5 0 33 0 0 0 2x Zoysia Westwood 38 98 100 100 100 100 Quickstand 43 100 100 100 100 100 Creeping B. 30 27 58 77 78 48 Perennial R. 47 62 82 93 95 97 Kentucky B. 22 0 8 0 0 15 Yukon 23 85 88 100 98 90 Primo 27 100 100 98 100 88 Primo + PRl 50 98 100 98 98 87 Primo + CB 35 100 100 98 98 77 Primo + KB 22 100 100 100 98 80 Primo + PR2 22 100 100 98 97 78 4x Zoysia 5 0 33 0 0 0 Westwood 38 98 100 100 100 87 Quickstand 43 100 100 100 100 92 Creeping B. 30 27 58 58 45 15 Perennial R. 47 62 82 87 92 78 Kentucky B. 22 0 8 0 0 2 75 Table 3. Turf color* observed in different species under traffic stress. 8/21/2001 9/24/2001 10/8/2001 11/2/2001 Yukon 8.3 8.5 5.6 1.7 Primo 7.0 7.5 4.8 1.0 Primo + PR1 7.5 8.7 6.8 6.4 Species Primo + CB 7.0 7.6 4.8 1.3 Primo + KB 7.0 7.7 4.8 1.3 Primo + PR2 7.3 8.2 5.3 2.3 Zoysia 0.0 0.0 0.0 0.0 Westwood 8.2 8.0 4.8 1.3 Quickstand 8.2 7.4 4.7 1.0 Creeping B. 2.5 7.8 6.7 5.8 Perennial R. 9.0 9.3 8.7 8.3 Kentucky B. 0.0 0.0 0.0 3.0 *Color ratings based on a scale of 1-10, where 10 is the most desirable green and 6 the least acceptable; 1 is completely discolorated grass Literature cited Carrow, R.N., R.R. Duncan, J.E. Worley and R.C. Shearman. 2001. Turfgrass traffic (soil compactation plus wear) simulator response of Paspalum vaginatum and Cynodon spp. p. 253-258. In K. Carey (ed.). Int. Turf. Soc. Research J. Vol. 9. Gaussoin, R.E., D.D. Minner, S. Keeley, and M. Vaitkus. 2001. Annual seeding of Cynodon dactylon (L.) Pers. For improved performance of heavily trafficked athletic fields in temperate climates p. 865-869. In K. Carey (ed.). Int. Turf. Soc. Research J. Vol. 9. 76 Kentucky Bluegrass Traffic Study M inner and V .JF alverde D.D. Objective To determine the effect that different traffic schedules have on Kentucky bluegrass performance. Specifically we were interested in determining if the same amount of traffic caused more injury if it was applied all-at-once (one day per week) or spread out over time (a little each day). Methods The study was conducted at the Horticulture Research Farm in Ames Iowa during summer and fall of 2001. Six different traffic regimes were applied to Kentucky Bluegrass (Table 1) with a GA-SWC Traffic simulator (Carrow et al., 2001). The experimental design was a randomized complete block with 7 treatments and 3 replications. Each small plot was 2 ft x 12 ft. Traffic simulation started on 1 August and ended on 7 November, 2001. Table 1. Traffic schedule followed on Kentucky bluegrass during fall 2001. Number of passes per day Number of passes/week Monday Tuesday Wednesday Thursday Friday 5 dispersed 1 1 1 1 1 10 dispersed 2 2 2 2 2 15 dispersed 3 3 3 3 3 5 concentrated 0 0 0 0 5 10 concentrated 0 0 0 0 10 15 concentrated 0 0 0 0 15 Control 0 0 0 0 0 Percent turf cover and quality were evaluated monthly. Sometimes conditions were too wet to operate the traffic simulator. Traffic not done during those days accumulated to the next available day. Results Table 2. Kentucky bluegrass cover as affected by traffic schedule Traffic schedule 8/28/01 9/11/01 9/24/01 10/8/01 11/21/01 Overall % Turf cover Control 100 100 100 100 100 100.0 5 dispersed 93 93 95 96 92 93.8 10 dispersed 73 73 75 83 63 73.7 15 dispersed 57 52 63 57 35 52.7 5 concentrated 98 96 97 95 95 96.1 10 concentrated 93 88 77 85 85 85.7 15 concentrated 63 62 50 50 55 56.0 Prob. 0.0000 LSD 0.05 2.83 77 Table 3. Kentucky bluegrass quality as affected by traffic schedule Traffic schedule 8/21/01 8/28/01 9/11/01 9/24/01 10/8/01 11/21/01 Overall 9.2 9.3 9.8 Quality Control 10.0 10.0 5 dispersed 8.3 8.3 8.0 8.7 7.7 6.8 8.0 10 dispersed 6.7 7.0 6.3 6.0 6.0 4.0 6.0 15 dispersed 6.7 5.7 4.7 5.7 3.7 2.3 4.8 10.0 10.0 5 concentrated 8.7 8.7 8.3 8.3 7.2 7.5 8.1 10 concentrated 8.0 7.7 7.3 6.7 6.3 6.3 7.1 15 concentrated 6.0 5.8 5.3 4.7 3.3 Quality 1-10(10 most desirable situation, 1 not suitable for sport) 3.3 4.8 Prob 0.0000 LSD o.o5 0.336 Turf cover decreased as the amount of traffic increased. At 10 and 15 passes per week more turf cover occurred with concentrated traffic compared to disperse traffic (table 2). Similar situation occurs on turf quality (table 3). Although this is not evident in the data, there may have been a complicating factor caused by the unpredictable rainfall events. Traffic treatments applied during wetter conditions may have caused more damage. In 2001 we will conduct the same levels of traffic under wet vs. dry conditions by controlling rainfall with tarps. Literature cited Carrow, R.N., R.R. Duncan, J.E. Worley and R.C. Shearman. 2001. Turfgrass traffic (soil compaction plus wear) simulator response of Paspalum vaginatum and Cynodon spp. p. 253-258. In K. Carey (ed.). Int. Turf. Soc. Research J. vol. 9. 78 Species Traffic Study F.J. Valverde and D.D. M inner Introduction Turfgrass species are often ranked according to their wear resistance from high to low: tall fescue > perennial rye > Kentucky bluegrass > fíne fescue > creeping bentgrass > colonial bentgrass > rough bluegrass. However recuperative capacity from high to low may rank: creeping bentgrass > Kentucky bluegrass > rough bluegrass > tall fescue > perennial rye > fíne fescue>colonial bentgrass (Turgeon, 2002). In practice however athletic fields are a system of management techniques and reestablishment by seeding or sodding. Instead of evaluating the rate at which an established species declines or recovers during traffic, we are interested in evaluating how the whole grass system, seeding included, responds to traffic. Basically, what is the net performance of a species when continual attempts at reseeding are considered. Objective To study the interaction between overseeding and traffic stress on established species. Methods The study was conducted at the Horticulture Research Farm in Ames Iowa. Seeded in September of 2000 this study consisted in establishing 6 different species before the end of the spring. After species were succesfully established, they received traffic stress with a GA-SWC Traffic simulator (Carrow et al., 2001). Each species received two different regimes of traffic, 2 and 4 passes 3 days per week. The trial followed a complete randomized block design for a total of 12 treatments and 4 replications. Each small plot has 2 ft x 15 ft. Traffic simulation started on 20 April and ended 15 May. Plots were reseeded after this traffic period. Traffic reinitiated 2 weeks later, stopped on 15 June and were reseeded again. Percent turf cover and turf color were evaluated on 28 June 2001. Table 1. Treatment description and seed rate Species Traffic level Seed rate lb/1000 sq.ft Unique Kentucky Bluegrass 2/4 passes 3 times per week 3 Catalina Perennial Rye 2/4 passes 3 times per week 10 Millenium Tall fescue 2/4 passes 3 times per week 10 Cindy Fine Fescue 2/4 passes 3 times per week 5 Poa Supina 2/4 passes 3 times per week 3 Penncross Creeping Bentgrass 2/4 passes 3 times per week 2 79 Results The preliminary data of 2001 appear in table 2. The study will be continued and a final report will be made in 2002. Table 2. Percent turf cover and turf color measured on 28 June 2001 after repeated traffic. % cover Color Species Unique Kentucky bluegrass. Traffic levels 2x 4x 2x 4x 94 84 8.6 8.4 Catalina Perennial Rye 84 74 8.9 8.9 Millenium Tall fescue 85 78 8.3 8.0 Cindy Red Fescue 81 74 8.0 7.5 Poa Supina 98 89 8.1 7.9 Penncross Creeping bentgrass. 70 8.0 81 7.8 *Color ratings based on a scale of 1-10, where 10 is the most desirable green and 6 the least acceptable; 1 is completely discolorated grass L iteratu re cited Turgeon, A.J. 2002. Turfgrass management, 6th ed. New Jersey. Prentice Hall. 400 p. Carrow, R.N., R.R. Duncan, J.E. Worley and R.C. Shearman. 2001. Turfgrass traffic (soil compactation plus wear) simulator response of Paspalum vaginatum and Cynodon spp. p. 253-258. In K. Carey (ed.). Int. Turf. Soc. Research J. voi. 9. 80 1991 Corn Gluten Meal Crabgrass Control Study - Year 11 B. R. Bingaman an d N. E. Christians Com gluten meal (CGM) has been screened for efficacy as a natural product herbicide and fertilizer in turf on the same plot since 1991. The study is being conducted at the Iowa State University Research Station north of Ames, IA in an area of'Parade' Kentucky bluegrass. The soil in this experimental area is a Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll) with an organic matter content of 4.6%, a pH of 6.45, 3 ppm P, and 144 ppm K. Individual experimental plots are 5 x 5 ft and there are 5 treatments with 3 replications. The experimental design is a randomized complete block. Com gluten meal is applied each year to the same plots at 0, 20, 40, 60, 80, 100, and 120 lbs/1000 ft2 (Table 1). Because com gluten meal is 10% N, these rates are equivalent to 0, 2, 4, 6, 8, 10, and 12 lb N/1000 ft2. The CGM is applied each year in a single early-spring preemergence application using 'shaker dispensers'. The materials are watered-in with the irrigation system. Supplemental irrigation is used to provide adequate moisture to maintain the grass in good growing condition. In 2001, applications were made on April 26. Turf quality was monitored from May 8 through September 13 (Tables 1 and 2). It was assessed using a 9 to 1 scale with 9 = best, 6 = lowest acceptable, and 1 = worst turf quality. Weed populations were measured by either counting the number of plants or estimating the percentage area coverage per individual plot. Crabgrass infestations were determined by counting the number of plants per individual plot. Plants in the 1 to 3 leaf stage were found in June 12 but the growth rate was very slow because of the high temperatures and low rainfall. The crabgrass plants were large enough to count by July 27 and subsequent data were taken on August 9, August 16, August 28, and September 13. Dandelion populations were assessed by counting the number of plants per individual plot. Clover populations were determined by estimating the percentage area of each plot covered by clover. Dandelion and cover data were taken from May 8 through September 13 (Tables 4 and 6). Data were analyzed with the Statistical Analysis System (SAS , Version 6.12) and the Analysis of Variance (ANOVA) procedure. Effects of CGM on turf quality and weed control were examined using Fisher’s Least Significant Difference (LSD) means comparison tests. To better express weed control, weed population data were converted to percentage reductions as compared to the untreated controls. At spring greenup in May, there were differences between treated and untreated turf and by mid May, the differences were more pronounced (Table 1). Quality differences between CGM treated turf and untreated turf were significantly different for the entire season but on some dates, the quality of turf treated at various CGM levels was not different from the untreated control. By mid to late August, turf quality deteriorated because of low rainfall amounts. Some rainfall improved turf quality by early September. Treatment with CGM resulted in numeric reductions in crabgrass populations for the entire season. The differences were not statistically significant because there were large variations in counts among the replications for the treatments (Tables 2 and 3). There was no crabgrass in turf treated at 40 lb/1000 ft2 and greater for the entire season (Table 2). In 2002, crabgrass control was equal to or better than in previous years at all CGM levels except at 20 lb/1000 ft2 (Table 8). Dandelion counts were significantly reduced by CGM for the entire season as compared to the untreated controls (Tables 4 and 5). Mean reductions were at least 92.3% for CGM at 40 lb/1000 ft2 and higher and greater than 96% for CGM at 60 lb/1000 ft2 and higher. In 2002, CGM at 20 and 40 lb/1000 ft2 reduced dandelion populations more than in 1997, 1998, 1999, and 2000 (Table 9). Dandelion control at the other CGM rates was equal to or greater than in previous years. Percentage clover cover was significantly reduced in turf treated with CGM as compared with the untreated controls for the entire 2002 season (Tables 6 and 7). Mean reductions in clover cover were > 77% as compared with the untreated controls in turf treated with CGM at all levels except 20 and 40 lb/1000 ft2. Clover control in 2001 was similar to that in previous years except at 40 lb/1000 ft2 (Table 10). Control at this CGM level was not as good as in 1994, 1996, 1997, 1998, 1999, and 2000. 81 üC _ en en o p en ro en G oo oo os Os q q q O O O O »ri vo i> oo as as on h o n o q q »ri vo i> »ri t-^ o© oo oo oo ^«N ^ O h O Is; h rn oo J3 S £o B c Z 03 H 03 'C jg os p 13 13 13 13 13 13 ¿3 h 00 ©* P © © © © © rr © © © © © 3§>2S © r - © © © © © < © P © © © © © o o o o o © k o o d o o rN ~ O t"» o o o o o © © © © © a 00 o £c o u 3 < o o o o o o © o o o o o © oo © © © Tt © c © p 3 £o in ' ro ro © © © © © oo ro o © o o o o ’cL JU 5 £ £^ © © ©©©©©©© (N T t VO OO © CN 1 3 3O P Vh O jg ^ p doo > ^^ a ^ S I £ 3 a s 3 .1 £ 5 £ 3 G- 3 ffi fg cd *£ s to p O C3 | £ £ £ £ £ £ £ C3 cG cC d> c3 0> I I| S o3 o3 3o o3 o3 3o C3 'o b o o *00 o o oo M n h a« aD 3§I, C a>Lcn o p O o o O O V VO vo IN 00 ON o »o O N ON ON ON ON CN CN n- bX) < 28 •n o On p ON o N ON vn 00 o' CN oo m ON ON oo ON ON in TO B v 3 da o O T6O 3 O E o U On ON .c TO O 13 p d CZ3 sCO bb 3 o T n vo N (U x Oh-S 3 & «n 'S O- co © Ui cd © §H C g Q pcd O V > c(U nJ U £ S ii £ ^ -H z 8 E a> 00 p oo p © ' O rn C/3 p X © iri © o ' p - p ffi p p in c n d ^ rn ^ d v i o -h ' GO _ -j 00 X (N vq T f ca3 On rT c n G o C N O o O O 33OO Nd C d d 00 00 d d < C NOn NVOvo OnO p cd vo Oo p60V o Tf d V O K i a> < 60 < 8 o > G 0> II £ cz> -H Z 3 c 1 O o C RA S O > g d o C O 11 d o o 3 ‘C O 0 V- GO o o o o o o Z' r-H 3 *“> d ¡2 « >> 3 3 3 oo in X X C A CA c «n p p p p © p p 3— ^ O OP p ov od pTt- pin p inNO in r- O nO N ^t in 3 May 3 3 p p CO CO o CO O p p r- p P 00 c d f- V vo »n in K Nrm O OOnO o CNO oVOo00 o o o o o C N 1 3*~ 3 3 3 G c B G (D B Tl w u 3 3 3 3 3 3 3(U 3 ) 3 ) 3 ) 3 ) 3 ) 3 ) 1~ E £ c oE oE E o o oE o D U U u u u u - CNCO in VOr- SN Percentage clover cover1 in Kentucky bluegrass treated in the 1991 Com Gluten Meal W eed Control Study. ON d s | T able 6. O o O 5ro 8! a « id g £ 2Oh G o P fli o X c o o RJ 8 00 > p X Os O s O s Os 0) 0> £ è c o O O ON (N Tt 0 \ (N r - oo in oc ü n (N n - o\ h O r - £ oo ov oo ov o a 0) £ o r-io o r-r- 3 OS 5 £ o O OO vo oo Os O Tf- vo tJ- e n oo r» M r* oo ov oo o s o U oo m io »o Tt en oo os r oo so ê G O v £ G M fe o y ja *-< oo 0\ en m *T) o OV ON O U O G 3 £ m oo oo n Tf o 00 Ov Ov ov ov o r- Os oo VO OO o os os o G 0 o Os Ti- fe 1 ex o #o o £ o *s O o o O oo VO h M J \ h >n oo o \ oo a T3 -o C ° Jw a fe t/3 *3 § * 3 3i~ ‘C G a O a o s T3 o 3 3 J a a a M a a a S3 3 cd Ö 0 b D b ú Ö0 OX) OO ¿ c E OE OE O EOEOEO £ O O U U O O 00 JU 3 oo o o os ex a g o o CX —< o o o a C 015 £ « _ o ex c » g a S cd cd > g 4> C - M n i - i n vo h -S o 1 1 •6 O C«+H £ oa -H 3 P E *c E E E E E a a a a a 3a 3) I £ £ -rt o U cd cd cd cd cd cd flJ O O O 1) a a 3a 3a 3) a 3) E E £ £ c o o o o o o D UU U U U U Q cn J Ov J» 3 — cN m Tt vn vo r- X h- Table 10. Comparisons of the mean percentage clover cover reductions1 in Kentucky bluegrass treated in the 1991 Com Gluten Meal Weed Control Study for 1994 through 2000. o o Gh N I s o o « C * ’ aj c a> td 2 o o o o o o (N Tt so O Ooo (N ’ o "3 Id Id J- s _ _ 5 ^ ^ _ OX) OX) M |c oe oe 0 0 ’OX) OX) o oe oe e o D U U O UU U S to o S ^ & _c 52 *3 o T3 % Oi G -h £ gG h cO 2 P b —rN po Tt i/s vo cd > J3 « CO 1995 Corn Gluten Meal Rate Weed Control Study - Year 7 B.R. Bingaman and N. E. Christians Com gluten meal (CGM) is being screened for efficacy as a natural product herbicide in turf. This long-term study was begun in 1995 at the Iowa State University Horticulture Research Station north of Ames, IA. The experimental plot is in established 'Ram 1' Kentucky bluegrass. The soil is a Nicollet (fine-loamy, mixed, mesic Aquic Hapludoll) with an organic matter content of 4.8%, a pH of 7.1, 6 ppm P, and 170 ppm K. Prior to treatment in 1995, the percentage broadleaf weed cover within the study perimeter exceeded 50%. The experimental design is a randomized complete block design. Individual experimental plots are 10 x 10 ft with three replications. Each year com gluten meal is applied to the same plots at a yearly rate of 40 lb CGM/1000 ft2 (equivalent to 4 lb N/1000 ft2) using four different regimes of single and split applications for a total of five treatments (Table 1). Four applications of 10 lb/1000 ft2, split applications of 20 lb/1000 ft2, an initial application of 30 lb plus a sequential o f 10 lb/1000 ft2, and a single application of 40 lb/1000 ft2 are included with an untreated control. Initial applications for 2001 were made on April 26 before crabgrass germination. It was 80° F and sunny with a slight wind. The second application of treatment 2 was made on June 25 under cloudy skies with a high temperature of 83° and a slight S wind. The third application of treatment 2 and the second of treatments 3 and 4 were made on August 6. It was 86° and sunny with a 10 mph wind from the south. There was no rain until August 4 and only 2.43" of rainfall for the entire month of August. The final application of treatment 2 was made on September 12. It was 75 deg and sunny with a southerly wind at 15-20 mph. Temperatures remained above normal for the entire spring and summer and rainfall amounts were below normal. The experimental plot was checked for phytotoxicity after each treatment. Turf quality data were taken weekly from spring greenup on May 8 through September 13. Visual quality was measured using a 9 to 1 scale with 9 = best and 6 = lowest acceptable, and 1 = worst quality (Table 1). Crabgrass was first observed on June 12 but because of the hot temperatures and low rainfall, the growth rate was very slow. Population assessments could not be made until July 27. Subsequent data were recorded on August 9, August 16, August 28, and September 13 (Table 2). Broadleaf data were taken from May 8 through September 13. Dandelion and clover were the predominate broadleaf weed species within the experimental plot. Dandelion populations were measured by counting the number of plants per plot (Tables 4). Clover infestations were estimated by determining the percentage area in each individual plot covered by clover (Tables 6). Data were analyzed with the Statistical Analysis System (SAS, Version 6.12) and the Analysis of Variance (ANOVA) procedure. Means comparisons were made with Fisher’s Least Significant Difference test (LSD). Crabgrass, clover, and dandelion population data were converted to percentage reductions as compared with the untreated controls (Table 3,5, and 7). The 2001 growing season was hot and extremely dry. As a result there was less response to the nitrogen in the com gluten meal than in previous years. Supplemental irrigation was used as required to keep the bluegrass from entering dormancy. In spite of the growing conditions, turf quality was significantly better in bluegrass treated with CGM than in the untreated control for most of the season (Table 1). By September 5, the turf was dormant but rainfall in late October resulted in significant greening so additional data were taken on October 27. On this date, turfgrass that received sequential applications (treatments 2, 3, and 4) had better quality than the untreated control and treated turf that received only an initial CGM treatment. Mean visual quality of bluegrass treated with CGM was better than the untreated controls. Crabgrass populations were reduced by CGM at all application rates as compared with the untreated controls (Table 2). The best crabgrass control was in turf treated with split applications of 20 lb CGM but this level of control was not statistically different from the other CGM treatments and the untreated control. Mean reductions in crabgrass counts were > 94% in turf treated with 2 applications of 20 lb CGM or 30 lb followed by 10 lb CGM as compared with the untreated control (Table 3). The level of crabgrass control was lower at 10 lb CGM (four applications) than in 2001 (Tables 8 and 9). Split applications of 20 lb CGM provided 100% crabgrass reductions in 2001 and 2000 as compared to 45, 33, 50, 86, and 95% in 1995, 1996, 1997, 1998, and 1999, respectively. A single 40 lb CGM application decreased crabgrass counts by 94% in 2001. Dandelion numbers were significantly lower in all CGM treated turf as compared with the untreated controls for the entire 2001 season (Table 4). The best dandelion control was in turf treated with split applications of 20 lb CGM but this level was not different from the other CGM treatments. Dandelion reductions were similar for all CGM treatments and ranged from mean reductions ranged from 72.5 to 83.4% (Table 5). Dandelion control was slightly lower in 2001 than in 2000 (Table 10). Split applications of 20 lb CGM provided 83% control in 2001 but the level was 85 and 88% in 1999 and 2000, respectively. 88 Clover cover was significantly lower throughout the season in all CGM treated bluegrass as compared with the untreated controls. Clover populations were significantly lower in all CGM treated turf than in untreated on all data collection dates (Table 6). Mean reductions for all CGM treatments were >91% and all were statistically different from the untreated controls (Table 7). Clover control in 2001 was approximately equal to the level of control recorded in 2000 and 1999 (Table 11). At all CGM treatment levels, clover populations were lower than in treated bluegrass for 1996 through 1998. 89 o o o n w oo oo oo p p p o p Table 1. Turf quality1 of Kentucky bluegrass treated with com gluten meal for the 1995 Com Gluten Meal Rate Weed Control Study. vo oo oo oo oo oo p p p r- o vo h «3 c T3 25-1 x> oo oo on ppppp vo oo oo oo o ¡o © © — © ^-(N -O X ) O z xa> Pi ^ © o ©n m © ¿2 o pc au < ad < aus X) §J ^ Q r—, CL © Tf © Tf‘ vo t— © Os oo ’—i 3 5o oo G ^ < © On © p —; O r i O ^ oo h O On 00 rn vo O +-» t/2 G G < © Tt © © un © in <© oo VO © O n 00 © oo 00 m oo i i o M 'S G ~ < © © vo O p rn © C> » §> O G < p p o 00 rn © rf in o o eoo cn G 00 On 3 < p p © p © o © in m o On oo p rn vo 3 > cG> CN *— O t"-; p p rn © vo VO vo rn vo O O OO z X © ^ X) === © — X © © © Tf •§ « © © © © aj ,--1 JS O * •§ o I -O O © CN X) = © © 3 U g G ID « 3 3b E O U 3 H) £ G O 4 3 3) £ O U X © == ^t © cn m 1 £ g § 3 ‘C to cd 3 c0 3 a> 6 c 0) a 00 c &> 8« 2 o CO •c o to CL C m w cfl E E E E c o o o o DU U UU — . § ji Gu © ^ G co T B LLh (L> D JrJ oo<£ is £ G a> © ^ a « < D C C l c0 o £ x o £ c o © a> ■* -> | Table 2. £ a CZ> 00 00 FH Tt oc iri 6 - cn CL G q o Q 3 CZ) £ J G S czi 2 Table 4. Dandelion counts1 in Kentucky bluegrass treated with com gluten meal in the 1995 Com Gluten Meal Rate Weed Control Study. 00 £?oc < " 1 = 00 3 s* P >T 3 Z-i CD 2 8 CO CO C N CO A u g 2 8 A u g p vo >n 0 1 o CN as d oo as in OS n * os OS CN p 00 OS VO OS VO OS CN T f OS CD co CO GO o P> cm ' © t> o m 3 CN n >n ^ CO c o o o CO o u co 00 — r - o m OS OS 1 P co CO 0 y—* CD i3 i r r -. VO p 3 00 •—> »n t j o r - CO r - o csl o ' in vo in C 1 l CCS CD £ o 00 OS 3 »—i d vo OS 00 OS o vq p 3 cn d in OS in OS o OO O ) C o o p p o CN CN CN in - © i-3 rt w-1 CN CN as OS 00 CN p in OS OS' p 00 00 CN CN 1o o *0 3 3 £ § ON 3 ) E c 3 CN •—> p o p in © CN t > co CO c o o o m vo -C 1 CD c P CN o CD o p r - as CN Os CO o ' I o OS vo §a ’O fi ‘o co r - o © © CO CO p 00 CN in i—* r r >—» 3 » ¿3 00 t— co r -* p O o vo 3 <£ o o o « o o o O £ £> £> O CN CCS 4> 6 6 £ B c u c B c o O CN o £ o cn o 2 p CCS CD CCS (D CCS CD CCS CD 3 o £ E E £ c G CD o -a 2 3 O o Ih CD > o jO l 0 1 00 m p OS OS 5 * o 3 o Jp £> 3 o CN d 3 ) CD 2 3 K CL) > O o o ^ i/J M n Vh B > O 2 § 40 CD g o NA Percentage clover cover1 in Kentucky bluegrass treated with com gluten meal in the 1995 Com Gluten Meal Rate Weed Control Study. C/3 Table 6. p 00 m o o o cn a hJ -c H Os o o oo o Tt- r- Os VO O O OO âû O O m Os Os o »o o en «s oo o o o o (N m O O O •ë* 3 bß t/3 £2 ê § U VO o O o r- wn vo o VO O 00 O C/3 Z TC 3D CD £CD ■è* I B c 03 ai o ~ en O O (N o oc n Tt oo r» «ri g .2 2 tT) .s © c« © Oc ©n en © £ O © O T3 ôû<2 5 S c -o g èc S P, 03 D CD D ”c<3L> £ £ c c c c 2 2 2 2 B 3 3 J3 ”03 13 ”03 0 2 2 DU z 303 - H U (N rc U S " & “ c3 03 g o ta c D CD bû Ic o2 o2 o2 2 o o jj r 03 ^ (D C ' 2 c3bû bû bû D OO O O £! S3 o « i g o ¿2 2?S (D T3 O g B c a. > o O o ^C N ~ £ U O U U in a C/3 J > g D fc £ c/3 Percentage dandelion count reductions1 in Kentucky bluegrass treated in the 1995 Com Gluten Meal Rate Weed Control Study for 1996 through 2001. Table 10. o o (N | VO © O r-n O «>s o Onn *Oh n a o r- r- in in On Os o 00 00 O On C /5 t"* oo oo On On On On £ § u TO3 n o x> © © © < N § | £ =2 £ § & © < © o n m £ l X> o Id 1cd 2 c O U —’ T3 c «> S « T3 _ to .£ p mO 1 1 o ^ -§ < u 2p »-H « h gps S* p © m o © 22 S ifo 00 On © o o © © © o a<3 ^y 5 a £ £ § © © © " | X © > > 0 g e p10 .£ in © J ii 1 « is IO ^s® a p is co o To3 « E cp a CO M Id a B £ cc c c a a ^3 a a 60 (50 C S) s ’® £a, oc cL> < g cu DO | c E o oE oB E o DO U U U cd cd > a - (N n t}- in £ ^ cd •8 | B S s td *60 0 0 OX) 00 | c E o oE oE oE D UU U U £03 H - (N m d >n s c §b O i3 ocd ocd ocd ocd f EE £ E Q C/3 hJ Anti-Desiccant Winter Protection of Creeping Bentgrass Putting Greens D.D. M inner a n d F .J Valverde Introduction The three major types of winter turf injury are direct low temperature stress, winter desiccation, and low temperature fungi. Frozen conditions in the absence of snow cover can cause a slow but constant lost of moisture. This type of winter injury known as desiccation is especially damaging during sunny and windy conditions. It is known that artificial barriers between the grass and the environment can positively decrease the physiological damage. Protective covers or tarps are often placed over the turf just prior to ground freeze and are not removed in the spring until the surface thaws. For most winter conditions turf covers speed spring green-up and reduce winter injury. An alternative to using plastic or any other kind of covers in turfgrass is the use of anti-desiccants. These substances decrease the rate at which plant tissue would lose water. The objective of this study was to determine the effect that anti-desiccants applied before winter would have on the quality of turfgrass at springtime. Materials and Methods The study was initiated November 15, 2001 at the Horticulture Research Station on a USGA sand based putting green containing a mature stand of ‘Penncross’ creeping bentgrass. The trial had eight treatments (Table 1) and 3 replications. Each treatment was applied using a C 0 2 sprayer on an area of 5 x 5 ft2. The Evergreen Turf Cover was placed on the same day that anti-desiccant treatments were applied. Table 1. Description of products and application rates. Treatments Rate (oz/1000ft2) Treatments Rate (oz/1000ft2) 1 GLAD 42 5 Transfi lm 8 2 GLAD 17 6 Wilt-Pruf 42 3 GLAD 11 7 Cover Evergreen ~ 4 GLAD 8 8 Control ~ No other practice or treatment was applied to the trial. A foot of snow was on top of the trial for about 90 days. The first rating of the trial was done the 26 of March, and every ten days after that, for a total of 4 ratings. Turf color was visually evaluated using a scale from 1-10, where 1 is white-brown color and 10 dark green. Results The winter of 2000 - 2001 produced record snow cover with just over 90 days of snow cover between December and March. The extensive snow cover eliminated any chance of injury from winter desiccation. Grey Snow Mold was generally extensive throughout the state, but there was only minimal injury on this particular bentgrass research site. Table 1 shows the summary of four evaluation dates in the spring. Turf color was used to evaluate the amount of winter injury as well as the rate of spring green-up. Normal spring green-up began during the first week of April. The untreated control and the Evergreen cover were used for comparison with the anti-desiccant materials. The Evergreen cover provided better turf color than the non-treated control. Lower turf color ratings for the anti-desiccant materials seemed to be associated with a lighter tan color of the turfgrass blades. There was no rate effect among the GLAD treatments. 96 Table 2. Turf quality for various anti-desiccant treatments. Higher values represent less winter injury. Turf Quality at each given date Treatm ent GLAD GLAD GLAD GLAD Transfilm Wilt-Pruf Evergreen cover Control LSD o.o5 Rate oz/sqft 42 17 11 8 8 42 — — 3/26 2.3 3.7 2.3 3.3 2.3 3.3 7.0 4.0 IS 4/4 2.7 3.0 2.3 3.3 2.3 2.7 7.0 4.0 2.3 4/14 4.3 4.3 3.7 5.0 3.7 4.7 7.0 5.0 2.2 4/24 5.0 4.7 4.7 5.7 4.3 5.7 8.0 5.0 1.7 Avg 3.5 4.2 3.2 4.3 3.3 4.1 7.2 4.6 1.7 While there were no significant differences between the control and any of the anti-desiccant materials, there appeared to be a non-statistical trend. This trend indicated that the anti-desiccant materials resulted in lower turf color ratings than the non-treated control. By 4 May all of the grass treated with anti-desiccants had recovered to a level equal to the non-treated control and the trend ceased to exist. The anti-desiccant materials used in this trial did not improve the spring performance of putting green turf following the winter. The winter of 2000-2001 did not produce winter desiccation conditions. 97 Direct Heat Stress Effects on Creeping Bentgrass D eying Li, D a vid D. Minner, Christians While direct high temperature damage is often suspected in the loss of creeping bentgrass on golf course greens in the summer, little data is available on this subject. It is known that as supraoptimal temperatures are reached, grasses become more susceptible to biotic- or abiotic- stresses. High soil temperatures inhibit many physiological activities of roots cells and disturb the normal functions of roots such as water uptake, nutrients uptake, hormonal transportation, and photosynthates distribution. High temperatures also cause leaf injuries and accelerate leaf senescence (DiPaola & Beard 1992; Huang, et al., 2000, 2001). Sometimes, soil temperatures can be so high that direct injury occurs to the plant. This type of injury is classified as "direct heat stress" ( Minner, 1981; Wehner & Watschke, 1981). The exact scenario under which direct heat stress happens is not clear. It has been documented that a temperature gradient can be established above a turfgrass cover and that the surface temperature of the mat can be significantly higher than the air temperature just above the surface (Waterhouse, 1950). The objectives of this study were to investigate whether "direct heat stress" occurs on creeping bentgrass maintained under green conditions in central Iowa, to identify the forms of damage that occur, and to describe quantitatively the conditions that lead to the damage. Materials and Methods The experiment was conducted at the horticultural research station north of Ames, IA. "Crenshaw" creeping bentgrass was established at a rate of 2 lbs seed/1000ft2 on Sept. 14, 2000, on a sand-based green constructed with 30 cm of sand on top of a 10 cm gravel layer. The green has a drainage system consisting of 10-cm-diam. drainage pipes placed at 10 m intervals. Starter fertilizer 1, 0.5, and 0.5 lbs/1000 ft2 of N, P2 O 5 , and K2 0 , respectively, was applied at the time of seeding. At the time of seeding, six 5’ by 5’ plots were established on the area by topdressing a 0.5-cm layer of Profile, Quick dry; Zeolite, Axis, mason sand, and a mixture of sand and peat (90% /10% v/v) over the surface. This was done to create conditions of different thermal properties on the surface of the green. The experiment was a randomized complete block design with three replications. Temperatures at three locations in each plot were measured at 0, 1 and inches with thermal couples every 15 minutes for three consecutive days (Aug 11 to Aug 15). Water content was measured with a time domain refelectometer (TDR) in the top 5 cm of the soil media. 6 Results and Discussion Mat temperature was 23.4 to 27.5 °F higher than the air temperature (Table 1). When the air temperature reached 86.9 °F°, the mat temperature reached 115 °F. We closely observed the creeping bentgrass shoots that were directly in contact with the soil surface and found that a banding of injured tissue occurred to many of the plants at this temperature. We refer to this phenomenon as "heat girdling". Some of this type of heat stress penetrated through surrounding leaves and sheaths, deeper into the center of the shoots and when the new leaf emerged, the girdling was observed on the emerging leaf blades (Fig. 1). Further investigation on turf plugs taken from these field plots and maintained in the greenhouse showed that heat girdling generally happens when soil mat temperature reached 118 °F (48 °C). Exact air temperature at which such soil mat temperature happens depends on the soil water content and soil thermal diffusivity. However, once the soil mat temperature gets to the threshold temperature, it takes less than one hour to cause heat girdling to the bentgrass shoots. Further research is needed to correlate air temperature and soil temperature for different root zone materials with different thermal properties and water holding capacities in order to predict threshold air temperatures and the amount of time required for direct heat stress. At this point we may conclude that direct heat stress such as heat girdling could occur during high temperature periods in central Iowa. We also believe that soil properties can affect the degree of injury. In the next phase of this work, we will address practical measures that can be taken to reduce heat stress damage on greens. 98 Table 1. Root-zone surface temperatures observed when air temperature reached 86.9 °F (30.5 °C) on August 12, 2001 . Sand 10% Peat/90% sand (v/v) Profile Axis Zeolite o 'n o Maximum mat temperature ✓— \ O Treatment 110.3(43.5) 112.1(44.5) 110.8(43.8) 114.4(45.8) 110.8(43.8) Volumetric Water content @ 5 cm % 12.6 16.4 15.1 12.9 14.6 Fig. Heat girdled band on youngest leaf shown 3 days after injury occurred on Axis treatment. Leaf continued to grow and injured area became visible. Sand-Based Sport Field Stability Study D eying Li, D.D. Minner, a n d N E Christians Sports turf is an important area of turfgrass application. Unlike golf courses, most of the sports turf fields require more stable playing surface to support the activities of players and facilities, and to provide protection against sports injuries. The quality of playing surface is a function of turfgrass and soil media (Canaway and Baker, 1993). Surface quality is usually expressed as friction, traction, stiffness, and resilience when the interaction between the surface and player is the main concern. It also can be evaluated from the ball bounce resilience and rolling resistance or ball speed when information about the behavior of sport facilities on a playing surface is needed (Bell et al., 1985; Baker et al., 1988; McClements and Baker, 1994). O f all the qualities of playing surface, perhaps the safety of the players is the most important consideration when constructing and evaluating a sports field. Many sports injuries are related to varying degree of surface stability (Valiant, 1988; Powell and Schootman, 1993; Waddington and McNitt, 1995). To assess the risk requires knowledge from many disciplines including the sports ground mechanics, which is not well understood because of very limited research. The objective of this study was to evaluate the relative importance of sand particle size, particle-size distribution, particle shape and roundness, plant roots and root-zone water content in the stability of a sand-based sport field surface. Materials and Methods The study was established on an existing sand-based sport turf area at the Horticultural Research Station. The root zones were excaved to form 5 X 10 ft plots 6 inches deep. Five treatments, Hallet mason sand, Hallet concrete sand, Sidlley Proangle sand, Bunker white sand, and Hallet mason sand + 15% soil (v/v) were filled in the plots and compacted with a Whacker vibrating compactor for ten passes. The experiment design is a randomized complete block, with three replications. Sand angle at repose, particle-size distribution, particle shape/roundness (Li, 2001) was analyzed before loading materials to the plots (Table 1). On June 25 2001, half of each plot was planted with washed sod and half seeded with Kentucky bluegrass 'Unique'. The turf has been mowed once a week at 5 cm. The turf received 159 kg ha'1N, 35 kg ha'1P, and 96 kg ha'1K in three months period after sodding. Water content was measured by TDR each time surface stability was evaluated. Playing surface parameters were evaluated both before and after turf establishment. The playing surface was evaluated by measuring the penetration with a penetrometer, surface hardness with a B&K 2500 vibration analyzer (Rogers and Waddington, 1990) and traction with a cleated torque wrench device (Canaway and Bell, 1986). Bulk density also was measured to determine the compaction status. Two month into the turf establishment grass root and thatch dry mass was quantified in addition to other measurements. Preliminary Results Adding soil to Hallet mason sand did not increase penetration resistance. Penetration resistance increased after sodding for all materials except silica sand (Table 2). On 27 July 2001 (32 days after sodding) there was a noticeable increase in GmaxA. Thereafter, GmaxA decreased with no explanation (Tablé 2). Hallet mason sand had significantly greater traction than Hallet concrete or silica sand. Adding soil to mason sand did not increase traction (Table 3). For each sand material there was an optimum water content that maximized penetration resistance. Sand source (angularity) played a more important role in maximizing penetration resistance than adding 15% soil to mason sand. Two months after sodding traction, measured by the torque wrench method, did not correlate with many of the plant mass or sand property measurements in this study (Table 3). 100 © © © © ^ © © © © c> — © o in © —' © rn on © m X fOvo *— « 00 VO in © vo © a I u c o Z fs| ^ r- T3 vo r~~ © VO in £ Table 1. Particle size analysis of the sand sources used in the study. os oo ’— © m C ts 2 © vo © © © > O i> oCfl vP c o 03 E ^ T3 1 0) 2 co JL> . 60 i 2 cu is 13 rs DC X oo 60 2o > < (U Xo cL o -o C cd 0> 260 © a H 2 _o 15 ao> -O 2 NxO o 'O § «« o ^ P C| o cu ~ z ^ 1 jd jd g jd ^ 13 3 ~ "5 DC DCc/3 c/o DC Q oo J Table 3. Correlation coefficients between sand properties, plant mass, and playing surface parameters measured on 25 Aug. 2001, two months after sodding. Conclusions At this point it appears that multiple parameters, as opposed to a single type of measurement, will be required to accurately assess the stability of sand-based sport fields. References Baker, S.W., A.R. Cole, and S.L. Thornton. 1988. Performance standards and the interpretation of playing quality for soccer in relation to rootzone composition. J. Sports Turf Res. Inst. 64:120-132. Bell, M.J., S.W. Baker, and P.M. Canaway. 1985. Playing quality of sports surfaces: a review. J.Sports Turf Res.Inst. 61:26-45. Canaway, P.M. and S.W. Baker. 1993. Soil and turf properties governing play. International Turfgrass Society Research Journal. 7:192-200. McClements, I. and S.W. Baker. 1994. The playing quality of rugby pitches. Journal of the Sports Turf Research Institute. 70:2943. Powell, J.W. and M. Schootman. 1993. A multivariate risk analysis of natural grass and astroturf playing surfaces in the National Football League 1980-1989. International Turfgrass Society Research Journal. 7:201-211. Valiant, G.A. 1988. Ground reaction forces developed on artificial turf. Science and football, p. 406-415. In: Reilly, T., Lees A., Davids, K. and Murphy, W.J. (ed). First world congress of science and football, Liverpool, 13-17 April 1987. E.&.F.M.Spon Ltd. 12 New Fetter Land, London EC4P 4EE; E. & F.M. Spon, 29 West 35th Street, New York, N Y 10001. Waddington, D.V.and A.S. McNitt. 1995. Penn State Research on surface characteristics of playing fields. The Keynoter. 23(2):5-7. 103 Iowa State University Personnel Affiliated with the Turfgrass Research Program Barbara Bingaman, Ph.D. Postdoctoral Research Associate, Horticulture Dept. Natalie Canier Graduate Student, M.S. (Minner) Nick Christians, Ph.D. Professor, Turfgrass Science Research and Teaching, Horticulture Dept. Lucas Dant Field Technician, Horticulture Dept. Will Emley Superintendent, Horticulture Research Station Shui-Zhang Fei, Ph.D. Assistant Professor, Turfgrass Science Research (Plant Breeding), Horticulture Dept. Mark Gleason, Ph.D. Professor, Extension Plant Pathologist, Plant Pathology Dept. MarkHelgeson Field Technician, Horticulture Dept. Mark Howieson Graduate Student, Ph.D. (Christians) Jeff lies, Ph.D. Associate Professor, Extension, Nursery Crops/Omamentals, Horticulture Dept. Young K. Joo, Ph.D. Visiting Scientist from Korea Jason Kruse Graduate Student, Ph.D. (Christians and Chaplin - starting Fall 2001) Donald Lewis, Ph.D. Professor, Extension Entomologist, Entomology Dept. Deying Li, Ph.D. Postdoctoral Research Associate (Christians and Minner) David Minner, Ph.D. Associate Professor, Turfgrass Science Research and Extension, Horticulture Dept. Hugh O’Donnell Field Research Technician Daniel Oschner Field Technician, Horticulture Dept. Troy Oster Graduate Student, M.S. (Christians) Rodney St. John Superintendent, Turfgrass Research Station, Horticulture Dept. Joe Stoeffler Field Technician, Horticulture Dept. Federico Valverde Research Associate, Horticulture Dept. 104 Companies and Organizations That Made Donations or Supplied Products to the Iowa State University Turfgrass Research Program Special thanks are expressed to the Big Bear Turf Equipment Company and Textron for providing a Cushman Turfgrass Truckster, and Ryan GA30 aerifier; to Tri-State Turf and Irrigation for providing a Greensmaster 3100 Triplex Greensmower and a Groundsmaster 345 rotary mower; and, to Great American Outdoor for providing a John Deere 2500 Triplex Greensmower for use at the research area. M icro F lo M ilorganite M onsanto Company O ssian Lnc. PBL/Gordon C orporation P ickseed W est Lncorporated P ro file P roducts R ainbird Lrrigation Company Rhone-Poulenc C hem ical Company R iverdale C hem ical Company Rohm a n d H aas Co. The Scotts Company Seeds W est Lnc. Standard G o lfCompany SubAir Syngenta Tee J e t Spray P roducts Terra C hem ical C orporation Textron The Toro Company T ri S tate T u rf& Lrrigation Co. True Pitch, Lnc. T u rfSeed, Lnc. U nited H orticultural Supply U nited Seeds Lnc. W eatherm atic C orporation W illiam s Lawn Seed Company A iken P ea t A ndersons Law n Tech A vends BASF B ecker U nderw ood B ig B ear T u rfE quipm ent Com pany C hem ical Services Labs D & K T u rfP roducts D ow A grosciences G ardens A live G o lfC ourse Superintendents A ssociation o f Am erica G reat A m erican O utdoor G reen a n d B io Tech, Lnc. H eatw ay H unter Lndustries, Lnc. Lowa G o lfC ourse Superintendents A ssociation Lowa P ro fessio n a l Law n Care A ssociation Lowa S ports T u rfM anagers A ssociation Lowa Turfgrass Lnstitute Jacklin S eed L E SC O Lncorporated 105