Search results

Title: Managing carrot foliar diseases in commercial production fields in Michigan
Creator: Donne, Irene Mariel
Date: 2017
Collection: Electronic Theses & Dissertations
Description: "Fungal foliar diseases caused by Alternaria dauci and Cercospora carotae occur annually on carrots. Our goal was to update the disease management tactics by: 1) Testing OMRI-approved and conventional fungicides and 2) Evaluating TOM-CAST. Trials were conducted in 2015 and 2016. Disease severity was visually assessed weekly using the Horsfall-Barratt scale and a petiole health scale; the area under the disease progress curve (AUDPC) was calculated for these parameters. Root yield was...
Show more"Fungal foliar diseases caused by Alternaria dauci and Cercospora carotae occur annually on carrots. Our goal was to update the disease management tactics by: 1) Testing OMRI-approved and conventional fungicides and 2) Evaluating TOM-CAST. Trials were conducted in 2015 and 2016. Disease severity was visually assessed weekly using the Horsfall-Barratt scale and a petiole health scale; the area under the disease progress curve (AUDPC) was calculated for these parameters. Root yield was determined at harvest. Based on AUDPC results obtained in 2015 and 2016, the copperbased fungicides (copper hydroxide and copper hydroxide + copper oxychloride) were the only OMRI-approved products that significantly and consistently limited foliar blight. On the final assessment dates in both years, all conventional fungicides limited foliar and petiole blighting compared to the control with one exception; the propiconazole treatment in 2016 was similar to the control for petiole health. Yields differed significantly among the conventional treatments in 2016 but not in 2015. All treatments yielded significantly higher than the control except for iprodione. Treatments of pyraclostrobin + boscalid, fluxapyroxad + pyraclostrobin, and boscalid had statistically higher yields than penthiopyrad, iprodione, and propiconazole. TOM-CAST 15 and 25 DSV fungicide application schedules effectively reduced foliar blighting in 2015 under relatively light disease pressure. However, the TOM-CAST 25 DSV schedule did not adequately limit disease in 2016 when disease pressure was increased. Recently registered fungicides such as penthiopyrad and fluxapyroxad + pyraclostrobin and using TOM-CAST at the more conservative spray threshold of 15 DSV can help growers limit fungal foliar blight in years with higher disease pressure."--Page ii.
Show less

Title: Social-ecological systems, values, and the science of "people management"
Creator: Piso, Zachary Amedeo
Date: 2017
Collection: Electronic Theses & Dissertations
Description: This dissertation interrogates a shift in environmental science, policy, and management toward conceptualizing the environment as a social-ecological system. Social-ecological systems science reflects an interdisciplinary effort to understand how individuals and communities achieve their environmental goals through the institutions that they maintain. Though the paradigmatic institutions concern economic behavior (e.g. property rights institutions), the field embraces the social sciences...
Show moreThis dissertation interrogates a shift in environmental science, policy, and management toward conceptualizing the environment as a social-ecological system. Social-ecological systems science reflects an interdisciplinary effort to understand how individuals and communities achieve their environmental goals through the institutions that they maintain. Though the paradigmatic institutions concern economic behavior (e.g. property rights institutions), the field embraces the social sciences broadly, with contributions from sociology, anthropology, geography, political science, and so on. That said, social science is fairly narrowly conceived; leaders in the field stress that they are studying social mechanisms in order to predict and manage social behavior. In a popular textbook on the subject, Fikret Berkes and Carl Folke stress that "resource management is people management" and call for a social science of this management.Social-ecological systems scientists have generally neglected the ethics of people management-for the most part they subscribe to a fairly typical fact/value dichotomy according to which scientists describe social-ecological systems while managers and policymakers prescribe actions in light of these descriptions. Following several philosophical traditions (in particular pragmatist philosophy of science), I call attention to the ways that social-ecological systems science is value-laden. I take environmental pragmatism to provide a roadmap for conducting social-ecological systems science ethically. Environmental pragmatists stress that science is always embedded in practical problem-solving activities that presuppose particular goals for, and side constraints to, inquiry. Many traditions in the philosophy of environmental science embrace social science for the specific role of facilitating this deliberation, but these traditions do not seem to anticipate the explanatory ambitions of social sciences. This leaves unaddressed several pertinent questions about how social explanations work (i.e. how functional distinction structure inquiry), which have very practical implications for which social science disciplines should be included in a collaboration and how social and ecological knowledge should be integrated. For example, most social situations are characterized by property rights institutions, cultural traditions, political alliances, and other social institutions within the purview of particular social science disciplines, but researchers are not reflexive about whether to explain environmental change according to one set of practices or another.The dissertation traverses the following terrain: the first chapter more carefully motivates the questions above regarding the need for ethics and the promise, but present inadequacy, of environmental pragmatism to meet this need. Chapter two attends to Dewey's theory of inquiry, in particular the dialogical dimension of inquiry that authorizes warranted assertions. Through reflection on Daniel Bromley's volitional pragmatism and a debate between Richard Rorty and hermeneutic social scientists, chapter three attends to the way that social science structures inquiry in order to intervene in the normative practices of a community. Chapter four analyzes social-ecological explanations in order to locate normative and evaluative assumptions that should be accountable to democratic deliberation. Finally, chapter five redescribes interdisciplinary integration as an ethical project where decisions about the centering and decentering of different sciences is as much ethical as epistemological.
Show less

Title: The meaning of teaching mathematics : teacher positionings as embedded in algebra teachers' guides
Creator: Suh, Heejoo
Date: 2017
Collection: Electronic Theses & Dissertations
Description: Teacher educators have been examining the professional status of teaching, including defining central practices of teaching, comparing teaching to other professions, and understanding teachers’ own perspective via interviews, surveys, and observations. The present study intends to contribute to the discussion by examining the meaning of teaching as reflected in teachers’ guides. I chose to examine teachers’ guides because they are known to have impact on teachers. Being a resource that is...
Show moreTeacher educators have been examining the professional status of teaching, including defining central practices of teaching, comparing teaching to other professions, and understanding teachers’ own perspective via interviews, surveys, and observations. The present study intends to contribute to the discussion by examining the meaning of teaching as reflected in teachers’ guides. I chose to examine teachers’ guides because they are known to have impact on teachers. Being a resource that is close to teachers’ everyday practices, curriculum materials inspire teachers with what they could do in their classroom. Educators intending large-scale educational reform pay attention to curriculum materials. Careful use of linguistic features therein could enhance thus further support teachers’ guides in effective communication with teachers.I investigated four 8 th grade Algebra teachers’ guides - TG, selectively chosen to represent variety. Drawing on positioning theory, I observed positionings regularly appearing in each of those four. Then I turned to idiosyncratically-observable positionings. Two research questions sought understanding of what teaching mathematics entails as presented in the TGs, hence how each guide constructs teaching mathematics. Last, I examined the guides’ linguistic features. This was for understanding the degree of teacher professional judgment acknowledged by the guides.My results indicated that, as constructed by each guide, teaching mathematics does not differ much across the guides. Those guides presented various types of knowledge as entailed in teaching mathematics. Compared to aspects on teacher professionalism in the literature, the guides occasionally addressed teacher research, interaction with colleagues, utilizing knowledge, and acknowledging uncertainty. In addressing these aspects, the guides in most cases did so with insufficient support. Examining idiosyncratic positionings, I found two types: i) positionings occurred idiosyncratic only, and ii) positionings occurred idiosyncratically in addition to their regular appearance. The latter can be classified into two types: a) one that succeeds the context of the communication actions associated with the general positioning, and b) one dissociated from the context. My linguistic examination of the guides suggested that they varied in their use of voice. Some are more directive, others are more suggestive, indicating different levels of acknowledgement of teachers’ professional judgment. In this dissertation’s last chapter, I present ways in which this study contributes to understanding of curriculum materials and of teacher professionalism. I end this dissertation with implications for curriculum authors, teachers, teacher educators, and researchers.
Show less

Title: Exploring the possibilities of Teachtown MKE : using local cultural institutions to support novice teachers' access to community resources
Creator: Shattuck, Tamara M.
Date: 2017
Collection: Electronic Theses & Dissertations
Description: Cultural institutions have the capacity to provide teachers with material resources for curriculum use in the classroom, professional support through connection to professional learning communities, promotion of self-confidence in teaching, and gains in subject content knowledge. There is building evidence that these types of support systems help novice teachers (i.e., teachers in their first three year of teaching), however many teachers do not have access to such support. And while access...
Show moreCultural institutions have the capacity to provide teachers with material resources for curriculum use in the classroom, professional support through connection to professional learning communities, promotion of self-confidence in teaching, and gains in subject content knowledge. There is building evidence that these types of support systems help novice teachers (i.e., teachers in their first three year of teaching), however many teachers do not have access to such support. And while access to resources is important for novice teachers, there is also evidence that access does not guarantee the use of resources. This descriptive study explores the inclusion of cultural institutions as partners in novice teacher support programs though the investigation of Milwaukee, Wisconsin's Key to the City Pass (KCP), which provides new teachers access to over 22 local cultural institutions. I also investigate how novice teachers develop and use the skill of relational agency -the ability to "work fluidly across professional boundaries" (Edwards, 2005a p. 177) and understand the utility of materials for problem solving. I hypothesize building the skill of relational agency helps novice teachers' better access KCP resources for professional use. Thus, four research questions drive this study; 1) How do teachers use KCP benefits? 2) Do teachers who participate in the KCP program incorporate community/cultural institution resources in their teaching practices, and if so how? 3) What barriers exist, and how can the KCP program be made more accessible for teachers? 4) Do participant teachers display the skill of relational agency, does this skill apply to KCP use? I used both quantitative and qualitative methods to analyze data and document teachers' use of the KCP through: (1) a teacher survey, (2) semi-structured focus group interviews, and (3) case study teacher interviews and artifact analysis. The quantitative survey data was analyzed first to gather general patterns of KCP use across participant teachers. I analyzed survey data using descriptive and inferential statistics. The qualitative data was analyzed in a second phase to gather information about how teachers use KCP resources. For the qualitative data analysis, I used deductive coding. Findings indicate that participants were mostly using the KCP program for personal reasons (e.g., trips with friends and family), and that they were able to connect with the local community through these trips. Through personal trips, teachers were also able to assess whether taking field trips to certain cultural institutions was appropriate for their students. Few teachers used the skill of relational agency in connection to the KCP and noted they were more likely to use cultural institution resources if they were clearly connected with state and district standards. Teachers were also more likely to use cultural institution resources if they had the opportunity to collaborate with cultural institution staff. This study enabled me to observe barriers to KCP use before generalizing how such a program might be created in other cities. Which in turn allowed me to understand the steps other cities should take in order to build stronger partnerships. Based on my findings of the barriers to KCP use I suggest cities who wish to form partnership programs: 1) gather input from all constituents, 2) create a strong mission statement, 3) connect to district wide initiatives, and 4) ensure a pathway for clear and continual communication between partners and participants.
Show less

Title: Solid-state nuclear magnetic resonance studies of the structures, membrane locations, cholesterol contact, and membrane motions of membrane-associated HIV Fusion Peptide (HFP
Creator: Jia, Lihui (Scientist)
Date: 2017
Collection: Electronic Theses & Dissertations
Description: Membrane fusion is the key step during HIV viral entry to cells, and the process is catalyzed by HIV membrane fusion protein gp41. HFP is the ~25-residue N-terminal domain of gp41 and is required for membrane fusion with significant decreases in fusion activity with point mutations. Both viral and host cell membrane contain ~30mol % cholesterol (CHOL), and HFP induced fusion is faster in membrane with CHOL. However, how HFP interacts with membrane lipids and CHOL is unknown. In this thesis,...
Show moreMembrane fusion is the key step during HIV viral entry to cells, and the process is catalyzed by HIV membrane fusion protein gp41. HFP is the ~25-residue N-terminal domain of gp41 and is required for membrane fusion with significant decreases in fusion activity with point mutations. Both viral and host cell membrane contain ~30mol % cholesterol (CHOL), and HFP induced fusion is faster in membrane with CHOL. However, how HFP interacts with membrane lipids and CHOL is unknown. In this thesis, we used the newly developed 13C-2H Rotational Echo Double Resonance (REDOR) solid-state NMR method to study the membrane location of HFP in chemically-native membrane environment. HFP is 13CO labeled at specific residue, and the membrane is deuterated at specific regions of the membrane using selective regions deuterated phosphatidylcholine (PC) and CHOL. We study HFP wild type, HFP_V2E and L9R mutants because these two mutants eliminate and decrease fusion respectively. HFP is predominantly β sheet structure in bilayer membrane for both HFP wild type and HFP_V2E mutant, HFP_L9R has a different structure and is likely helical. Both HFP and HFP_V2E mutant have major deeply-inserted membrane location contacting membrane center and minor shallowly-inserted membrane location contacting half way of one membrane leaflet. The HFP_V2E mutant has bigger fraction of molecules with shallower membrane location, which is consistent with the strong correlation between membrane location insertion depth and the peptide fusogenicity. HFP_L9R mutant has majorly deeply inserted into membrane.By comparing the HFP- PC and HFP- CHOL contact, there is preferential contact between HFP and CHOL vs PC at several residues including G5, G10 and G16. The free energy difference for contacting PC vs CHOL is ~ 0.57(5) kcal.mol-1 for T= 300K. HFP- CHOL contact geometry is successfully modeled by Swiss Dock and YASARA energy minimization with two strands antiparallel HFP (1→16/16→1 registry). There are two energetically favorable binding models between HFP and CHOL, from docking, energy minimization and consistency with REDOR results. The contact models reveal tilted and curved-up tail orientation of Chol_d7. Fusion may be catalyzed by matching the curvature of lipids contacting HFPs with the membrane curvature during the fusion intermediates like the stalk. Membrane motion perturbation by HFP is studied by static deuterium NMR from deuterium powder pattern spectrum, order parameter profile and T2 relaxation time. The DMPC-d54 spectrum becomes ~10% narrower in membrane without CHOL with 4% HFP and in membrane with 33% CHOL with 1% HFP. Accordingly, the order parameter of lipid acyl chain becomes ~ 1-10% disordered by HFP. However, the spectrum becomes 20% broader in membrane with 33% CHOL with 4% HFP, and the order parameter of lipid acyl chain becomes ~ 20- 30% ordered by HFP. With HFP at 37 °C, DMPC-d54 T2 decreases ~ 70 %, and the CHOL T2 decreases ~ 30%. T2 reduction is probably associated with increased membrane curvature induced by HFP. With greater membrane curvature, the C-D bond will experience more orientation diversity relative to the external magnetic field. Thus, the quadrupolar field will have greater change, leading to faster relaxation and shorter T2. Gp41_V2E mutant eliminates cell-cell fusion. Our CD spectroscopy studies show that the FPHM_V2E mutant is helical and the melting temperature is above 90 °C in 10mM Tris buffer + 0.2 % SDS at pH 7.4. Protein is trimer and induces no lipid mixing in PC: CHOL= 2:1 vesicles.
Show less

Title: The carbon sequestration and soil respiration after land use conversion in biofuel cropping ecosystems
Creator: Su, Yahn-Jauh
Date: 2017
Collection: Electronic Theses & Dissertations
Description: Global climate change alters Earth's carbon, hydrological and energy cycles from local to global scales, changing our climate patterns and impacting our lifestyles and prosperity. The development of bioenergy may partially mitigate the release of carbon dioxide during the combustion of fossil fuel. However, the carbon emissions from the bioenergy-induced land use change have long been debated and it is not certain whether they really represent a reduction of carbon emission. In this study, I...
Show moreGlobal climate change alters Earth's carbon, hydrological and energy cycles from local to global scales, changing our climate patterns and impacting our lifestyles and prosperity. The development of bioenergy may partially mitigate the release of carbon dioxide during the combustion of fossil fuel. However, the carbon emissions from the bioenergy-induced land use change have long been debated and it is not certain whether they really represent a reduction of carbon emission. In this study, I monitored the components of the net ecosystem exchange (NEE) of CO2, including gross primary production (GPP), ecosystem respiration (Reco), total soil respiration (Rs), autotrophic soil respiration (Ra) and heterotrophic soil respiration (Rh), to understand their responses to climate variability and in particular a severe drought event. I studied three major bioenergy crops (continuous corn, switchgrass and restored multicultural prairie) on fields with two different land use histories (conventional corn-soybean rotation and Conservation Reserve Program brome grass fields). I found that the amplitude, the duration and the seasonality of microclimatic variables (temperature and precipitation) were important for the carbon dynamics in the bioenergy cropping systems. The soil water content affected the annual NEE, GPP and Reco although it did not have strong correlations with these components of carbon fluxes at short-term scale. The short-term (1-2 week) normal summer water deficit may affect annual NEE while long-term (spring-summer) drought may change the community structure and affect the carbon cycling processes in the following years. The temperature sensitivities of soil respiration were shifted within and between years. In addition, crop types and land use histories affect the responses of ecosystem to climate events. The different phenology between annual and perennial crops and the establishment of dense root systems in perennial crops can change the ratio of the components of NEE and change the direction and the amounts of net ecosystem carbon flux. Annual and perennial crops have different strategies responding to different climate scenarios and their combinations. The monitoring of climate patterns at intra-annual scale is required to understand how the ecosystem respond to climate change.
Show less

Title: Dynamic network analysis with applications to functional neural connectivity
Creator: Golibagh Mahyari, Arash
Date: 2017
Collection: Electronic Theses & Dissertations
Description: "Contemporary neuroimaging techniques provide neural activity recordings with increasing spatial and temporal resolution yielding rich multichannel datasets that can be exploited for detailed description of anatomical and functional connectivity patterns in the brain. Studies indicate that the changes in functional connectivity patterns across spatial and temporal scales play an important role in a wide range of cognitive and executive processes such as memory and attention as well as in the...
Show more"Contemporary neuroimaging techniques provide neural activity recordings with increasing spatial and temporal resolution yielding rich multichannel datasets that can be exploited for detailed description of anatomical and functional connectivity patterns in the brain. Studies indicate that the changes in functional connectivity patterns across spatial and temporal scales play an important role in a wide range of cognitive and executive processes such as memory and attention as well as in the understanding the causes of many neural diseases and psychopathologies such as epilepsy, Alzheimers, Parkinsons and schizophrenia. Early work in the area was limited to the analysis of static brain networks obtained through averaging long-term functional connectivity, thus neglecting possible time-varying connections. There is growing evidence that functional networks dynamically reorganize and coordinate on millisecond scale for the execution of mental processes. Functional networks consist of distinct network states, where each state is defined as a period of time during which the network topology is quasi-stationary. For this reason, there has been an interest in characterizing the dynamics of functional networks using high temporal resolution electroencephalogram recordings. In this thesis, dynamic functional connectivity networks are represented by multiway arrays, tensors, which are able to capture the complete topological structure of the networks. This thesis proposes new methods for both tracking the changes in these dynamic networks and characterizing or summarizing the network states. In order to achieve this goal, a Tucker decomposition based approach is introduced for detecting the change points for task-based electroencephalogram (EEG) functional connectivity networks through calculating the subspace distance between consecutive time steps. This is followed by a tensor-matrix projection based approach for summarizing multiple networks within a time interval. Tensor based summarization approaches do not necessarily result in sparse network and succinct states. Moreover, subspace based summarizations tend to capture the background brain activity more than the low energy sparse activations. For this reason, we propose utilizing the sparse common component and innovations (SCCI) model which simultaneously finds the sparse common component of multiple signals. However, as the number of signals in the model increases, this becomes computationally prohibitive. In this thesis, a hierarchical algorithm to recover the common component in the SCCI model is proposed for large number of signals. The hierarchical recovery of SCCI model solves the time and memory limitations at the expense of a slight decrease in the accuracy. This hierarchical model is used to separate the common and innovation components of functional connectivity networks across time. The innovation components are tracked over time to detect the change points, and the common component of the detected network states are used to obtain the network summarization. SCCI recovery algorithm finds the sparse representation of the common and innovation components of signals with respect to pre-determined dictionaries. However, input signals are not always well-represented by pre-determined dictionaries. In this thesis, a structured dictionary learning algorithm for SCCI model is developed. The proposed method is applied to EEG data collected during a study of error monitoring where two different types of brain responses are elicited in response to the stimulus. The learned dictionaries can discriminate between the response types and extract the error-related potentials (ERP) corresponding to the two responses."--Pages ii-iii.
Show less

Title: Dual role of PU.1 in enhancer priming in macrophages
Creator: Tagore, Mohita Malay
Date: 2017
Collection: Electronic Theses & Dissertations
Description: All multicellular organisms arise from a single-celled zygote by the precise execution of a gene expression program which ensures appropriate cell identity. This process is particularly challenging in eukaryotic cells since eukaryotic DNA is packaged by architectural proteins called histones into chromatin, which might act as a barrier to the transcriptional machinery. Macrophages are cells of the immune system which undergo rapid, large scale changes in gene expression in response to...
Show moreAll multicellular organisms arise from a single-celled zygote by the precise execution of a gene expression program which ensures appropriate cell identity. This process is particularly challenging in eukaryotic cells since eukaryotic DNA is packaged by architectural proteins called histones into chromatin, which might act as a barrier to the transcriptional machinery. Macrophages are cells of the immune system which undergo rapid, large scale changes in gene expression in response to bacterial or viral challenge. This makes macrophages an excellent model for studying cell-type specific as well as inducible gene expression. Studies at the genome-wide level have shown that distal regulatory elements like enhancers play an essential role in determining the macrophage inducible response to microbial challenge. Further, lineage-specific transcription factors like PU.1 and C/EBPβ are known to bind inducible enhancers prior to gene induction in resting macrophages. Earlier studies using genome-wide approaches indicate that PU.1 is able to interact with chromatin, thus functioning as a 'pioneer factor' in macrophages. However, not much is known about the mechanism by which PU.1 keeps enhancers accessible prior to gene induction in resting macrophages. Using bone-marrow derived primary mouse macrophage cells as well as PU.1 deficient cell lines, my work highlights the changes in chromatin associated with PU.1 binding during macrophage differentiation as well as in response to bacterial infection. Using a quantitative nucleosome occupancy assay, we reported that PU.1 binding correlates with low nucleosome occupancy at an inducible enhancer in resting macrophages. Further upon induction with an appropriate stimulus, nucleosomes are stably evicted from the distal enhancer and the corresponding gene can be induced. More importantly, my results suggest that lack of PU.1 binding renders regulatory regions (enhancers and promoters) of inducible genes susceptible to heterochromatin formation and silencing by Polycomb repressive complex 2 (PRC2) in differentiated macrophages. PRC2-mediated silencing is also associated with an increase in nucleosome occupancy at the target regions and the corresponding genes cannot be induced. Results obtained from this research will provide important insights into the role of lineage-specific transcription factors at regulatory elements both during normal development and disease.
Show less

Title: Protease-containing membranes for rapid, controlled antibody digestion prior to mass spectrometry analysis
Creator: Pang, Yongle
Date: 2017
Collection: Electronic Theses & Dissertations
Description: "Monoclonal antibodies are the fastest growing class of therapeutic drugs because of their high specificities to targeQt cells. Facile analysis of therapeutic mAbs and their post-translational modifications (PTMs) is essential for quality control, and mass spectrometry (MS) is the most powerful tool for antibody characterization. Conventional antibody characterization workflows contain an in-solution digestion step, which is labor-intensive and time-consuming. Protease-containing membranes...
Show more"Monoclonal antibodies are the fastest growing class of therapeutic drugs because of their high specificities to targeQt cells. Facile analysis of therapeutic mAbs and their post-translational modifications (PTMs) is essential for quality control, and mass spectrometry (MS) is the most powerful tool for antibody characterization. Conventional antibody characterization workflows contain an in-solution digestion step, which is labor-intensive and time-consuming. Protease-containing membranes are an attractive alternative platform for protein digestion because of their high local enzyme concentrations, short radial diffusion distances, rapid convection in pores, simple fabrication and low cost. Additionally, variation of protein residence time in the membrane gives control over the size of proteolytic peptides. This research focuses on developing workflows for monoclonal antibody characterization using functionalized porous membranes. Sequential adsorption of poly (styrene sulfonate) and pepsin in a porous nylon membrane forms a pepsin membrane reactor. Pepsin is inexpensive and catalyzes proteolysis in acidic solutions, which avoids the need to alkylate cysteine residues and limits antibody deamidation. Variation of the residence times (3 ms to 3 s) of antibody solutions in pepsin-containing membranes yields "bottom-up" (1-2 kDa) to 'middle-down' (5-15 kDa) peptides in less than 10 min. These peptic peptides cover the entire sequences of Herceptin and a WatersTM antibody. Compared with the performance of bottom-up (in-solution tryptic digestion) and top-down (intact protein fragmentation) analysis of an antibody light chain, middle-down (in-membrane peptic digestion) analysis gives the highest bond cleavage (99%). In-membrane digestion also facilitates detection of PTMs such as oxidation, deamidation, N-terminal pyroglutamic acid formation and glycosylation. Recently developed protease-containing spin membranes provide an excellent platform for rapid, membrane-based protein digestion prior to ultrahigh-resolution Orbitrap MS analysis. Centrifugation of 100-200 æL of pretreated protein solutions through the pepsin- or trypsin-containing membranes takes less than 1 min and gives nearly 100% coverage of the protein sequences in subsequent direct infusion MS analysis of digests of apomyoglobin and four commercial monoclonal antibodies (Herceptin, Avastin, Rituxan and Vectibix). MS analysis of peptic and tryptic peptides also reveals mAb PTMs such as N-terminal pyroglutamate formation, C-terminal Lysine clipping and glycosylation. Liquid chromatography coupled to tandem mass spectrometry analysis of tryptic spin digests and subsequent MaxQuant data searching show 100% sequence coverage of all four antibody light chains, and 75.1%-98.4% coverage of the heavy chains. Compared to in-solution tryptic digestion of mAbs, spin digestion yields higher sequence coverage and a larger number of unique peptides. In-membrane digestion also facilitates protein sequence comparison. Rapid peptic in-membrane digestion of two antibodies with direct infusion MS analysis accurately reveals the antibody modification site in less than 1 h. Overall, membrane-based protein digestion uses minimal sample preparation time and yields high peptide and sequence coverages for identification of protein PTMs."--Page ii-iii.
Show less

Title: Where the rivers come together : reclaiming and re-imagining indigenous history, identity, and language in the city
Creator: Haviland, Adam
Date: 2017
Collection: Electronic Theses & Dissertations
Description: The movement and migration of Native Americans to urban areas is usually traced to the urbanization programs of the 1950s and 1960s. However, the recent scholarship of Coll Thrush (2007) and John Low (2016) highlight the long history of urban spaces as Indigenous spaces and the role Indigenous people and communities have played in their growth and development. Similarly, the discourse of language loss and revitalization, urban spaces, and Indigenous urban communities are seen as places or...
Show moreThe movement and migration of Native Americans to urban areas is usually traced to the urbanization programs of the 1950s and 1960s. However, the recent scholarship of Coll Thrush (2007) and John Low (2016) highlight the long history of urban spaces as Indigenous spaces and the role Indigenous people and communities have played in their growth and development. Similarly, the discourse of language loss and revitalization, urban spaces, and Indigenous urban communities are seen as places or endpoints of assimilation. Thus, language revitalization efforts and programs often focus on reservations as the primary domains where Indigenous languages and their speakers persist and thrive. Yet, despite settler colonial narratives of vanishing that erase Indigenous people and Indigenous languages from urban areas, cities have, and continue to be, important intersections of movement and migration and with deep historical roots where Indigenous languages persist and thrive as ideological markers of identity, belonging, and as spoken languages.This Research shows how Lansing, Michigan, Nkwejong (the place where the rivers come together) has a long history as an Indigenous intersection and space that challenges the local settler-colonial narratives of removal and erasure. Lansing has remained an Indigenous space through traditions of movement and migration that were driven by the auto industry and educational opportunities. Through these movements, Anishinabek from reservations in and around Manitoulin Island came here in the 1960s and 1970s who were fluent speakers ofAnishinaabemowin. Anishinabek from Canada and local Anishinabek, who had lost the language, created community and belonging through educational programs. These spaces have become focal points where community comes together and, for many individuals, are the primary spaces where language, culture, and identity are reclaimed and passed on. However, these are also spaces of tension where gender roles, language ideologies, and linguistic practices concerning language as an ideological marker of identity and its role as a communicative system are challenged and reimagined.Through interviews with community members and participant observation, I explore relationships to urban and reservation “homelands,” the importance of education as places where individuals develop relationships to their identities and culture, and the role that language, as both an ideological marker of identity and belonging and as a communicative system, play in their everyday lives and experiences. While most participants agreed that language was important to preserving identity and traditional knowledge, their relationships with their identities as urban and Indigenous, and their relationships to Indigenous language, highlight: (1) the need to reexamine language ideologies that link Language to “traditional culture and Knowledge” and the impacts these ideologies have on language revitalization. (2) The importance of urban areas as Indigenous homelands and places where Indigenous languages persist, and (3) the role of education as intersections and places of tension where multiple ideologies, identities, and ways of being Indigenous are expressed and reimagined.
Show less

Title: Feasibility and application of a CuxO-based memristor for sensing oxygen and other gases
Creator: Nyenke, Chinwe Pamela
Date: 2017
Collection: Electronic Theses & Dissertations
Description: This dissertation introduces the design, fabrication, and application of acopper-oxide-based memristor for the passive sensing of oxygen and other gases.The device design was as follows: Deposition of copper (Cu) bottom electrodes,(oxygen) vacancy-rich copper oxide (CuxO) switching layers, and tungsten (W) topelectrodes in a crossbar array structure. The CuxO layer was deposited via reactivesputtering of a Cu target with an argon-oxygen (Ar/O2) mixture. A portion of thislayer was extended...
Show moreThis dissertation introduces the design, fabrication, and application of acopper-oxide-based memristor for the passive sensing of oxygen and other gases.The device design was as follows: Deposition of copper (Cu) bottom electrodes,(oxygen) vacancy-rich copper oxide (CuxO) switching layers, and tungsten (W) topelectrodes in a crossbar array structure. The CuxO layer was deposited via reactivesputtering of a Cu target with an argon-oxygen (Ar/O2) mixture. A portion of thislayer was extended from each array cell to be exposed for sensing. Memristivedevices of different switching layer thicknesses were initially explored forirreversible sensing of oxygen in ambient air. Results of this first experimentdemonstrated an increase in resistance states upon prolonged exposure toambient air. For the second experiment, memristive devices were fabricated withsub-micron holes that were etched into the W top electrode to better reveal theswitching layer surface. The devices were also subjected to ambient oxygen at 180deg C to induce passive sensing in minutes. Resistance results were consistentwith the first experiment but also revealed a dependence on the surface area ofthe exposed oxide. Finally, memristive devices were investigated in a thirdexperiment for reversible sensing of an oxidizing gas and reducing gas at roomtemperature. This time, changes were not only observed in resistance but also inhysteresis (current versus voltage) depending on the type of gas introduced.Overall, this work demonstrates a step towards the use of the memristor as a gassensor, which we have named “memsensors”, by taking advantage of the device’sability to memorize (or record) historical information.
Show less

Title: Preservice teachers' intention to teach media and information literacy in their future classroom : an application of the theory of planned behavior
Creator: Gretter, Sarah
Date: 2017
Collection: Electronic Theses & Dissertations
Description: "This dissertation is a multi-phase study looking at preservice teachers' intention to teach Media & Information Literacy in their future classroom. Each of the three studies presented in this dissertation answered a specific question: 1) What do preservice teachers think about teaching MIL? 2) What predicts preservice teachers' intention to teach MIL? and 3) How can we support preservice teachers' intention to teach MIL? The first paper in this dissertation reported on an elicitation study...
Show more"This dissertation is a multi-phase study looking at preservice teachers' intention to teach Media & Information Literacy in their future classroom. Each of the three studies presented in this dissertation answered a specific question: 1) What do preservice teachers think about teaching MIL? 2) What predicts preservice teachers' intention to teach MIL? and 3) How can we support preservice teachers' intention to teach MIL? The first paper in this dissertation reported on an elicitation study conducted with focus groups of preservice teachers to understand, from their perspectives, the factors that would either impede or facilitate the teaching of MIL in their future classroom. The second paper described the design, validation, and results of a survey based on these factors. The third paper reported on an online module with reflective exercises designed around the results gathered in the aforementioned survey. Each paper describes the findings that emerged from its study, followed by implications for research and practice, along with questions for the field of Media & Information Literacy education"--Page ii.
Show less

Title: Role of a alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor on methylmercury-induced calcium dysregulation on motor neurons
Creator: Colón-Rodríguez, Alexandra
Date: 2017
Collection: Electronic Theses & Dissertations
Description: Methylmercury (MeHg) is a persistent environmental neurotoxicant to which humans are exposed mainly through the consumption of fish. MeHg leads to neuronal cell death in acute or chronic exposure and its mechanism of toxicity is not yet understood. Due to its high prevalence in the environment and its mechanism of toxicity MeHg has been considered a possible contributor to the development of Amyotrophic Lateral Sclerosis (ALS). Alterations in glutamate reuptake and Ca2+ regulation in ALS and...
Show moreMethylmercury (MeHg) is a persistent environmental neurotoxicant to which humans are exposed mainly through the consumption of fish. MeHg leads to neuronal cell death in acute or chronic exposure and its mechanism of toxicity is not yet understood. Due to its high prevalence in the environment and its mechanism of toxicity MeHg has been considered a possible contributor to the development of Amyotrophic Lateral Sclerosis (ALS). Alterations in glutamate reuptake and Ca2+ regulation in ALS and after MeHg exposures have been well documented. Most importantly, MeHg-induced alterations in intracellular Ca2+ ([Ca2+]i) in motor neurons lead to early onset ALS-like phenotype in the superoxide dismutase 1 (SOD1-G93A) mouse, a mouse model genetically susceptible to ALS. One of the ion channels that contribute to the alterations in [Ca2+]i observed in ALS and after MeHg exposure is the ionotropic glutamate receptor α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA). My research has focused on characterizing the effects of MeHg on motor neurons in vitro. I used two cell lines: a mouse motor neuron hybrid cell line NSC34 and an induced pluripotent stem cell derived-motor neuron (hiPSC-MN) cell line of human origin. I investigated MeHg toxicity in these cell types and on AMPA receptors in order to understand the role these ion channels play in the observed alterations in [Ca2+]i. Results from the studies in this dissertation demonstrate that MeHg exposure in vivo or in vitro lead to alterations in the AMPA receptor and the RNA editing enzyme ADAR2 gene expression. Also, that hiPSC-MNs are more susceptible than NSC34 cells to MeHg toxicity observed as an earlier concentration dependent cell death. I also identified that MeHg induces a bi-phasic increase in [Ca2+]i in hiPSC-MNs and Ca2+ permeable AMPA receptors are mediating those increases. Taken together these results suggest a potential role of the AMPA receptors in MeHg-induced toxicity in MNs. These findings contribute to the understanding of MeHg-induced toxicity in motor neurons and provide a platform for ongoing studies in our lab which are focused on identifying the underlying mechanisms by which MeHg is contributing to the accelerated onset of ALS-like phenotype.
Show less

Title: Experimental and numerical investigation of confined premixed flame
Creator: Najim, Younis Mahal
Date: 2017
Collection: Electronic Theses & Dissertations
Description: Understanding the dynamics of premixed flames propagating during constant volume combustion is key to enhancing the performance of existing combustion devices, which provide 80% of the world’s energy supply, and reducing the impact of pollution on the environment. This work experimentally and numerically investigates confined premixed flame propagation in an initially quiescent mixture. Three combustion chambers are used; a curved wave disc engine channel and rectilinear channels of aspect...
Show moreUnderstanding the dynamics of premixed flames propagating during constant volume combustion is key to enhancing the performance of existing combustion devices, which provide 80% of the world’s energy supply, and reducing the impact of pollution on the environment. This work experimentally and numerically investigates confined premixed flame propagation in an initially quiescent mixture. Three combustion chambers are used; a curved wave disc engine channel and rectilinear channels of aspect ratio 7 and 10. The mixture is methane/air and syngas (H2/CO)/air initially at atmospheric pressure and room temperature. The channel walls are assumed to be isothermal to incorporate the effect of heat transfer. For two-dimensional analysis, the reaction rate is modeled using both detailed and reduced kinetic mechanisms. The mass diffusion is investigated using three different diffusion models with different levels of approximation; the multicomponent diffusion model of Chapman-Enskog including the Soret effect; the mixture-averaged model; and constant Lewis number. For three-dimensional analysis, a large eddy simulation coupled with the transport equation of the reaction progress variable is used. In this work, the reaction rate predicted using the Boger model of algebraic flame surface density is modified by incorporating a transient flame speed that accounts for the variation in the temperature and pressure of the unburned gases. The experimental measurements include schlieren photography to track the flame structure and propagation speed, and the pressure-time history during the combustion process is measured by a pressure sensor mounted in the channel wall. The experimental measurements validate the numerical simulation results and provide further understanding of the flame and pressure dynamics. Unlike behavior previously reported in straight or 90◦ bend channels, premixed flame propagation in the wave disc engine channel exhibits different features: the convex tulip flame converts back into a concave flame and thus reveals the influence of channel geometry on flame evolution. The experiments show that the rate of pressure change eventually becomes negative mainly due to heat losses that engender a correspondingly slower flame propagation during the final stage of burning. The analysis of the numerical results reveals the effect of the interaction between the flame front, pressure field, and flame-induced flow on flame evolution during all stages of flame structure development. The results also demonstrate that both multicomponent diffusion with the Soret effect and the mixture-averaged model produce slightly different results in flame speed, structure, peak temperature, and average pressure for the methane/air mixture, while the deviation is more pronounced for syngas flames. The methane/air flame produced by the unity Lewis number model, however, lags behind its counterparts during early stages and dramatically accelerates, at which time the values of peak temperature and average pressure show unrealistic behavior. Furthermore, unity Lewis number flames develop an artificial second tulip flame after the first tulip flame is annihilated. This second tulip flame is neither observed in the Chapman-Enskog and mixture-average simulations, nor in the experiments. This reveals the role of the Lewis number in the intrinsic thermodiffusive flame instabilities and tulip flame formation. The three-dimensional simulation uncovers an interesting behavior for the flame structure that is introduced here as a “transverse tulip” flame, which has not been previously reported. The “transverse tulip” flame evolves in the direction perpendicular to that of the initial tulip flame after the latter undergoes the transition from cusped convex back to the concave finger shape. The commonly used Zimont model produces an unrealistically diffused flame front. The large eddy simulation coupled with the here-modified algebraic flame surface density overcomes this issue and reproduces the experimental observations of the flame structure, pressure-time history, and burning time with good agreement.
Show less

Title: Fluid animation on deforming surface meshes
Creator: Wang, Xiaojun (Graduate of Michigan State University)
Date: 2017
Collection: Electronic Theses & Dissertations
Description: "We explore methods for visually plausible fluid simulation on deforming surfaces with inhomogeneous diffusion properties. While there are methods for fluid simulation on surfaces, not much research effort focused on the influence of the motion of underlying surface, in particular when it is not a rigid surface, such as knitted or woven textiles in motion. The complexity involved makes the simulation challenging to account for the non-inertial local frames typically used to describe the...
Show more"We explore methods for visually plausible fluid simulation on deforming surfaces with inhomogeneous diffusion properties. While there are methods for fluid simulation on surfaces, not much research effort focused on the influence of the motion of underlying surface, in particular when it is not a rigid surface, such as knitted or woven textiles in motion. The complexity involved makes the simulation challenging to account for the non-inertial local frames typically used to describe the motion and the anisotropic effects in diffusion, absorption, adsorption. Thus, our primary goal is to enable fast and stable method for such scenarios. First, in preparation of the material properties for the surface domain, we describe textiles with salient feature direction by bulk material property tensors in order to reduce the complexity, by employing 2D homogenization technique, which effectively turns microscale inhomogeneous properties into homogeneous properties in macroscale descriptions. We then use standard texture mapping techniques to map these tensors to triangles in the curved surface mesh, taking into account the alignment of each local tangent space with correct feature directions of the macroscale tensor. We show that this homogenization tool is intuitive, flexible and easily adjusted. Second, for efficient description of the deforming surface, we offer a new geometry representation for the surface with solely angles instead of vertex coordinates, to reduce storage for the motion of underlying surface. Since our simulation tool relies heavily on long sequences of 3D curved triangular meshes, it is worthwhile exploring such efficient representations to make our tool practical by reducing the memory access during real-time simulations as well as reducing the file sizes. Inspired by angle-based representations for tetrahedral meshes, we use spectral method to restore curved surface using both angles of the triangles and dihedral angles between adjacent triangles in the mesh. Moreover, in many surface deformation sequences, it is often sufficient to update the dihedral angles while keeping the triangle interior angles fixed. Third, we propose a framework for simulating various effects of fluid flowing on deforming surfaces. We directly applied our simulator on curved surface meshes instead of in parameter domains, whereas many existing simulation methods require a parameterization on the surface. We further demonstrate that fictitious forces induced by the surface motion can be added to the surface-based simulation at a small additional cost. These fictitious forces can be decomposed into different components. Only the rectilinear and Coriolis components are relevant to our choice of local frames. Other effects, such as diffusion, adsorption, absorption, and evaporation are also incorporated for realistic stain simulation. Finally, we explore the extraction of Lagrangian Coherent Structure (LCS), which is often referred to as the skeleton of fluid motion. The LCS structures are often described by ridges of the finite time Lyapunov exponent (FTLE) fields, which describe the extremal stretching of fluid parcels following the flow. We proposed a novel improvement to the ridge marching algorithm, which extract such ridges robustly for the typically noisy FTLE estimates even in well-defined fluid flows. Our results are potentially applicable to visualizing and controlling fluid trajectory patterns. In contrast to current methods for LCS calculation, which are only applicable to flat 2D or 3D domains and sensitive to noise, our ridge extraction is readily applicable to curved surfaces even when they are deforming. The collection of these computational tools will facilitate generation of realistic and easy to adjust surface fluid animation with various physically plausible effects on surface."--Pages ii-iii.
Show less

Title: Affective aesthetics and the social politics of Neoliberalism in New Extremism cinema
Creator: Clark, Cameron
Date: 2017
Collection: Electronic Theses & Dissertations
Description: This thesis investigates the aesthetics of transgression in New Extremism cinema for the ways in which they inform, and are mutually informed by, neoliberal affect and social politics. Although negative affect and spectatorial embodiment are often central to critical discourse on New Extremism, this thesis instead attends to the interlocking aesthetic, narrative, and spatio-temporal expressions of affect as they correlate with neoliberal politics of respectability and reproduction. By...
Show moreThis thesis investigates the aesthetics of transgression in New Extremism cinema for the ways in which they inform, and are mutually informed by, neoliberal affect and social politics. Although negative affect and spectatorial embodiment are often central to critical discourse on New Extremism, this thesis instead attends to the interlocking aesthetic, narrative, and spatio-temporal expressions of affect as they correlate with neoliberal politics of respectability and reproduction. By deploying feminism and queer theory, this thesis locates positive, productive potentials within formal expressions of negative affect that mobilize a political critique of the violences within each film. In so doing, the selected film examples--Fabrice Du Welz's Calvaire (2004), Marina de Van's Dans ma peu (2002), Olivier Assayas's Demonlover (2002), Bruno Dumont's Twentynine Palms (2004), François Ozon's Criminal Lovers (2001), Alain Guiraudie's Stranger by the Lake (2014), to name a few--portray the shifting relations between politics, gender, and sexuality in the neoliberal present and offer critical alternatives for how such developments have impacted and impeded sociality.
Show less

Title: Effects of dimensions of word knowledge and their accessibility on different levels of reading comprehension in adolescent EFL learners
Creator: Choi, Yunjeong
Date: 2017
Collection: Electronic Theses & Dissertations
Description: Researchers have investigated that different dimensions of word knowledge (breadth versus depth) may play distinct roles in second language (L2) reading comprehension. Yet, little research has addressed how learners’ efficiency of accessing those dimensions of knowledge functions in their comprehension (i.e., the issue of knowledge availability versus accessibility in Cremer and Schoonen, 2013), not to mention comprehension at different levels. To fill gaps in previous research and enrich our...
Show moreResearchers have investigated that different dimensions of word knowledge (breadth versus depth) may play distinct roles in second language (L2) reading comprehension. Yet, little research has addressed how learners’ efficiency of accessing those dimensions of knowledge functions in their comprehension (i.e., the issue of knowledge availability versus accessibility in Cremer and Schoonen, 2013), not to mention comprehension at different levels. To fill gaps in previous research and enrich our knowledge about the lexical basis of L2 reading comprehension, the present study examined how different dimensions of L2 word knowledge and their respective accessibility function concurrently in explaining different levels of L2 reading comprehension in adolescent English as a Foreign Language (EFL) learners in Korea. To achieve this research goal, the present study defined lexical competence by distinguishing between vocabulary breadth and depth knowledge as well as between knowledge availability and accessibility. A number of tasks were employed to measure participants’ lexical competences: three paper-and-pencil tests for knowledge availability and three researcher-developed, computer-based tests for knowledge accessibility documenting response accuracy as well as latency. Reading comprehension was measured with the Gates-MacGinitie Reading Comprehension Test (MacGinitie & MacGinitie, 1992), which assessed both literal and inferential comprehension. In addition, the participants’ working memory and grammatical knowledge were also measured. The following key research findings were revealed. First, Confirmatory Factor Analysis analyses confirmed that knowledge of individual word meanings (breadth availability) and the ability to activate that knowledge efficiently (breadth accessibility), and knowledge of meaning relations among words (depth availability) and the ability to access that knowledge efficiently (depth accessibility) are distinctive constructs under the conceptualization of lexical competence. Second, availability and accessibility of depth knowledge were the only predictors that made significant contributions to reading comprehension when the impacts of those of breadth knowledge and working memory were controlled for. Within vocabulary depth, availability played a more important role than did accessibility. Availability and accessibility of breadth knowledge made no unique contribution to reading comprehension over and beyond those of depth knowledge. Third, the patterns of predictive roles of each lexical competence in reading comprehension did not seem substantially different between literal and inferential comprehension, confirming a stronger impact of depth knowledge, both availability and accessibility, than of breadth knowledge, and of availability of depth knowledge than of accessibility. However, the involvement of depth knowledge, particularly availability, seemed greater in inferential comprehension than in literal comprehension. These findings enriched our understanding about the lexical basis of L2 reading comprehension, particularly, the importance of efficient access to semantic network knowledge for textual reading and potentially differential involvement of semantic network knowledge in different levels of comprehension. Pedagogically, they suggested that vocabulary instruction should provide a learning environment where new words are provided in a meaningful reading context so that learners can establish a semantic network in their lexicon and continue developing, expanding, and consolidating the lexicon as they learn new words.
Show less

Title: Transparent multijunction organic photovoltaics
Creator: Young, Margaret (Process engineer)
Date: 2017
Collection: Electronic Theses & Dissertations
Description: "The widespread adoption of conventional solar cells based on inorganic semiconductors has been gaining traction in recent years but is still often hindered by high cost and lack of aesthetic appeal. Transparent organic semiconductor-based solar cells that selectively absorb in the UV and the NIR enable integration into building windows, automobiles, and consumer electronics in ways that traditional solar cells cannot. Moreover, integration onto existing infrastructure reduces the racking and...
Show more"The widespread adoption of conventional solar cells based on inorganic semiconductors has been gaining traction in recent years but is still often hindered by high cost and lack of aesthetic appeal. Transparent organic semiconductor-based solar cells that selectively absorb in the UV and the NIR enable integration into building windows, automobiles, and consumer electronics in ways that traditional solar cells cannot. Moreover, integration onto existing infrastructure reduces the racking and installation cost. In this work, we investigate routes to improve the efficiency of transparent solar cells by utilizing multijunction architectures. A transfer-matrix optical interference model is developed as a framework to optimize the full device stack considering the angle-dependent PV performance that is critical for matching subcell photocurrents in series tandem solar cells. In addition, a new method of fine tuning energy levels of low-bandgap small molecules with infrared selective absorption was demonstrated using a series of organic heptamethine salts. By exchanging the counterion from a small, hard anion to a fluorinated weakly coordinating anion, the frontier energy of the salt is shown to shift without affecting the bandgap, thus enabling simultaneous optimization of photocurrent generation of photovoltage. We further utilize this tunability to develop heptamethine molecules with absorption as deep as 1600 nm, the deepest infrared photo-response demonstrated to date with organic small molecules ideal for multijunction integration. Ultimately, transparent solar cells are an exciting new paradigm for solar deployment enabled by organic and excitonic semiconductors that offer a pathway to integrate solar onto virtually any surface without impacting the view."--Page ii.
Show less

Title: Microwave Imaging Using a Tunable Reflectarray Antenna and Superradiance in Open Quantum Systems
Creator: Tayebi, Amin
Date: 2017
Collection: Electronic Theses & Dissertations
Description: Theory, experiment, and computation are the three paradigms for scientific discoveries. This dissertation includes work in all three areas. The first part is dedicated to the practical design and development of a microwave imaging system, a problem mostly experimental and computational in nature. The second part discusses theoretical foundations of possible future advances in quantum signal transmission. In part one, a new active microwave imaging system is proposed. At the heart of this...
Show moreTheory, experiment, and computation are the three paradigms for scientific discoveries. This dissertation includes work in all three areas. The first part is dedicated to the practical design and development of a microwave imaging system, a problem mostly experimental and computational in nature. The second part discusses theoretical foundations of possible future advances in quantum signal transmission. In part one, a new active microwave imaging system is proposed. At the heart of this novel system lies an electronically reconfigurable beam-scanning reflectarray antenna. The high tuning capability of the reflectarray provides a broad steering range of +\- 60 degrees in two distinct frequency bands: S and F bands. The array, combined with an external source, dynamically steers the incoming beam across this range in order to generate multi-angle projection data for target detection. The collected data is then used for image reconstruction by means of time reversal signal processing technique. Our design significantly reduces cost and operational complexities compared to traditional imaging systems. In conventional systems, the region of interest is enclosed by a costly array of transceiver antennas which additionally requires a complicated switching circuitry. The inclusion of the beam scanning array and the utilization of a single source, eliminates the need for multiple antennas and the involved circuitry. In addition, unlike conventional setups, this system is not constrained by the dimensions of the object under test. Therefore the inspection of large objects, such as extended laminate structures, composite airplane wings and wind turbine blades becomes possible. Experimental results of detection of various dielectric targets as well as detecting anomalies within them, such as defects and metallic impurities, using the imaging prototype are presented.The second part includes the theoretical consideration of three different problems: quantum transport through two different nanostructures, a solid state device suitable for quantum computing and spherical plasmonic nanoantennas and waveguides. These three physically different systems are all investigated within a single quantum theory; the effective non-Hermitian Hamiltonian framework. The non-Hermitian Hamiltonian approach is a convenient mathematical formalism for the description of open quantum systems. This method based on the Feshbach projection formalism provides an alternative to popular methodssuch as the Feynman diagrammatic techniques and the master equation approach that are commonly used for studying open quantum systems. It is formally exact but very flexible and can be adjusted to many specific situations. One bright phenomenon emerging in the situation with a sufficiently strong continuum coupling in the case when the number of open channels is relatively small compared to the number of involved intrinsic states is the so-called superradiance. Being an analog of superradiance in quantum optics, this term stands for the formation in the system of a collective superposition of the intrinsic states coherently coupled to the same decay channel. The footprint of superradiance in each system is investigated in detail. In the quantum transport problem, signal transmission is greatly enhanced at the transition to superradiance. In the proposed solid state based charge qubit, the superradiant states effectively protect the remaining internal states from decaying into the continuum and hence increase the lifetime of the device. Finally, the superradiance phenomenon provides us a tool to manipulate light at the nanoscale. It is responsible for the existence of modes with distinct radiation properties in a system of coupled plasmonic nanoantennas: superradiant states with enhanced and dark modes with extremely damped radiation. Furthermore, similar to the quantum case, energy transport through a plasmonic waveguide is greatly enhanced.
Show less

Title: A detailed study of the gamma-ray nova V1324 Sco
Creator: Finzell, Thomas Michael Broen
Date: 2017
Collection: Electronic Theses & Dissertations
Description: It has recently been discovered that some, if not all, classical novae emit GeV gamma-rays, but the mechanisms involved in the production of the gamma-rays are still not well understood. We present here a comprehensive, multi-wavelength dataset---from radio to X-rays---for the most gamma-ray luminous classical nova to-date, V1324 Sco. Using this dataset, we show that V1324 Sco is a canonical dusty Fe-II type nova, with a reddening of E(B-V) = 1.16 +\- 0.12, a distance > 6.5 kpc, a bulk ejecta...
Show moreIt has recently been discovered that some, if not all, classical novae emit GeV gamma-rays, but the mechanisms involved in the production of the gamma-rays are still not well understood. We present here a comprehensive, multi-wavelength dataset---from radio to X-rays---for the most gamma-ray luminous classical nova to-date, V1324 Sco. Using this dataset, we show that V1324 Sco is a canonical dusty Fe-II type nova, with a reddening of E(B-V) = 1.16 +\- 0.12, a distance > 6.5 kpc, a bulk ejecta velocity of 1150 +/- 40 km/s and an ejecta mass of 2.0 +\- 0.4 * 10^-5 M_{Solar}. However, despite its seeming normalcy, there is also evidence for complex shock interactions, including the aforementioned gamma-rays and early time high-brightness temperature radio emission. To explain how a nova can be simultaneously ordinary and have the highest gamma-ray luminosity to date, we present a simplified model of the ejecta in which the strength of gamma-ray emission is set by properties of a fast ejecta component that collides with a slower component to produce shocks. We conclude this detailed study of V1324 Sco by showing how it has helped shape our understanding of the role of shocks in novae. Along with the study of V1324 Sco, this work also presents detailed methods for determining the reddening, distance, and filling factor of a classical nova from optical spectroscopy (using V1324 Sco as an example). We also provide detailed derivations for fitting nova radio light curves, to determine ejecta mass and velocity.
Show less

MSU Libraries

Digital Repository

Enabled Filters

Pages

Pages

MSU Libraries

Digital Repository

You are here

Search results

Pages

Pages