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Title: Evaluation of Operational Performance and Environmental Impact of a Commercial Scale Anaerobic Digester Utilizing Multiple Feedstocks
Creator: Dwilaksono, Fahmi
Date: 2022
Collection: Electronic Theses & Dissertations
Description: Food waste and livestock manure become some of major sources that contribute to greenhouse gas (GHG) emissions in the U.S. Utilizing manure and food wastes as biogas feedstocks through anaerobic digestion (AD) process can improve renewable energy production while reducing the impact of climate change due to GHG emission from untreated organic wastes. This study evaluated the operational performance of Michigan State University’s commercial South Campus Anaerobic Digester (SCAD) as well as the...
Show moreFood waste and livestock manure become some of major sources that contribute to greenhouse gas (GHG) emissions in the U.S. Utilizing manure and food wastes as biogas feedstocks through anaerobic digestion (AD) process can improve renewable energy production while reducing the impact of climate change due to GHG emission from untreated organic wastes. This study evaluated the operational performance of Michigan State University’s commercial South Campus Anaerobic Digester (SCAD) as well as the environmental impact during its operation in 2014-2020. Evaluation of feedstock supplies quantity and output parameters of SCAD was conducted to understand the operational performance of the digester. A life cycle assessment (LCA) was done to know the environmental impact of SCAD by comparing it to the conventional waste management methods. Technoeconomic analysis was conducted to know the financial feasibility of SCAD as a commercial digester. The result shows that during its operation from 2014 to 2020, SCAD has processed 159,145 metric tons of feedstock from 18 different organic wastes to produce 15,165,156 kWh of electricity for MSU community. LCA results show that AD system possesses fewer environmental burdens in both global warming potential (GWP) and water eutrophication potential (WEP) compared to the conventional system. Technoeconomic analysis reveals that SCAD needs 21.5 years to accomplish its payback time, which is considered quite economically competitive.
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Title: INTENSITY IN TEACHING : DEVELOPING AN AFFECTIVE FRAMEWORK FOR WRITING INSTRUCTORS
Creator: Chapman Ball, Cadaxa
Date: 2022
Collection: Electronic Theses & Dissertations
Description: This qualitative research project aims to develop an affective framework for writing pedagogy through analyzing narrative data collected over the course of one semester teaching MSU’s first-year writing course, WRA 101: Writing As Inquiry. The narrative data consists of journal entries and reflections remarking on my own affective states in the writing classroom. Through analyzing these writings, this thesis reveals how and why affect theory has useful applications in writing pedagogy; namely...
Show moreThis qualitative research project aims to develop an affective framework for writing pedagogy through analyzing narrative data collected over the course of one semester teaching MSU’s first-year writing course, WRA 101: Writing As Inquiry. The narrative data consists of journal entries and reflections remarking on my own affective states in the writing classroom. Through analyzing these writings, this thesis reveals how and why affect theory has useful applications in writing pedagogy; namely, that instructors who attend to their own affective responses have the opportunity to better understand the changes they need to make to their teaching practices as well as the systems in which they teach.
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Title: PERCEPTIONS OF VOICE PRODUCTION IN HEALTHY FEMALES IN REGARDS TO AGE AND PROFESSIONALISM : AS QUANTIFIED BY ACOUSTIC AND PERCEPTUAL MEASURES
Creator: Nizami, Hafsaah Fatima
Date: 2022
Collection: Electronic Theses & Dissertations
Description: PURPOSE: This study aims to research the perception of voice as it relates to aging and professionalism in an individual. The production of voice varies in accordance with various factors. These variations often provide an insight into a listener’s perceptions of a speaker’s vocal characteristics and these perceptions may vary across settings. METHODS: This study required four components. The first component required college aged participants to rate perceptions of age and health in regards...
Show morePURPOSE: This study aims to research the perception of voice as it relates to aging and professionalism in an individual. The production of voice varies in accordance with various factors. These variations often provide an insight into a listener’s perceptions of a speaker’s vocal characteristics and these perceptions may vary across settings. METHODS: This study required four components. The first component required college aged participants to rate perceptions of age and health in regards to young and old female vocalists. The second section required college aged participants to rate professionalism in regards to young and old female vocalists. Speech Professionals were asked to participate in the third component of this study, in which they rated listeners among the GRBAS scale. The last component required a quantification of results using a standard acoustic measure like cepstral peak prominence. RESULTS: Strong positive correlations were found between estimated age and actual age, strong negative correlations were found between older women and professionalism; moderately positive correlations were found between younger women and professionalism; moderate to strong correlation was found between age and GRBAS; and moderate negative correlations were found between age and CPPS. DISCUSSION: The following results were found: College-aged students were able to distinguish between the younger and older females. Older women were perceived as less professional. As age increases, GRBAS scores also increase. As age increases, CPPS ratings will decrease. CONCLUSION: Further research may require completion of surveys in a controlled environment, and inclusion of pitch-related measures as they relate to the workplace. Keywords: Aging voice, biological sex differences, perception of voice, professional voice, acoustic measures
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Title: PRETERM DELIVERY AND ITS ASSOCIATION WITH FALSE POSITIVE, AUDITORY BRAINSTEM RESPONSE (ABR)-BASED NEWBORN HEARING SCREENING FINDINGS
Creator: Rathore, Mandavni
Date: 2022
Collection: Electronic Theses & Dissertations
Description: Newborn hearing screening failure can occur in infants without hearing loss; these false-positive (FP) results have been speculated to reflect neurodevelopmental disorder risk. Preterm birth (PTB), a known neurodevelopmental risk factor, has been associated with FP at initial screening. We aim to further characterize this association by stratifying PTB by gestational age and delivery circumstance. To do this, we analyzed birth certificate and Early Hearing Detection & Intervention data from...
Show moreNewborn hearing screening failure can occur in infants without hearing loss; these false-positive (FP) results have been speculated to reflect neurodevelopmental disorder risk. Preterm birth (PTB), a known neurodevelopmental risk factor, has been associated with FP at initial screening. We aim to further characterize this association by stratifying PTB by gestational age and delivery circumstance. To do this, we analyzed birth certificate and Early Hearing Detection & Intervention data from the Michigan Dept. of Health & Human Services (2007–2015; n = 919,363). We restricted our analysis to singleton live births with available ABR-based hearing screening data and obstetric estimates of gestational age (n = 655,079). We then used logistic regression to evaluate the association of PTB defined by gestational age (extreme: < 28 weeks; moderate: 28–34 weeks; late: 34–36 weeks) and delivery circumstance (spontaneous, medically indicated) with FP, using full-term birth (≥ 37 weeks) as the referent group. Approximately 4% of infants had FP findings. All gestational age categories were associated with this phenomenon (extreme: OR = 4.2, 95% CI 3.7, 4.7; moderate: OR = 1.2, 95% CI 1.1, 1.3; late: 1.6, 95% CI 1.5, 1.7). Spontaneous and medically indicated PTB were also associated with FP (OR = 1.7, 95% CI 1.6, 1.8; OR = 1.4, 95% CI 1.3, 1.5, respectively). All results persisted following adjustment for socio-demographic and antepartum factors except for moderate PTB (OR = 1.0, 95% CI 0.9, 1.1), though sensitivity analyses suggested marked heterogeneity within this group. Further research is needed to investigate factors underlying these differences and whether they correlate with neurodevelopmental disorder diagnoses.
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Title: HOW CAREGIVERS OF CHILDREN IN EARLY INTERVENTION FEEL ABOUT SIMPLIFIED LANGUAGE INPUT : A SURVEY STUDY
Creator: Nylund, Megan
Date: 2022
Collection: Electronic Theses & Dissertations
Description: There is some disagreement regarding how adults should simplify their utterances when speaking to young children with language delays. One type of simplification that has come into question is telegraphic input, a style of speaking that involves removing grammatical aspects of phrases, such as function words and grammatical markers (e.g., All done snack, Cookie yummy). In the current study, we utilized survey data collected by Andary (2020) surveying caregivers of children in early...
Show moreThere is some disagreement regarding how adults should simplify their utterances when speaking to young children with language delays. One type of simplification that has come into question is telegraphic input, a style of speaking that involves removing grammatical aspects of phrases, such as function words and grammatical markers (e.g., All done snack, Cookie yummy). In the current study, we utilized survey data collected by Andary (2020) surveying caregivers of children in early intervention about their views on different types of simplified language. Specifically, we asked: How do caregivers’ beliefs about telegraphic input relate to how comfortable they are producing different types of simplified utterances? Results were also analyzed for correlations between demographics and beliefs of simplification. Additionally, the survey provided space for caregivers to share recommendations they gained through Early On resources. Results show caregivers with a positive view of telegraphic input felt significantly less comfortable producing full, grammatical utterances than caregivers with a negative or neutral view of telegraphic input. Demographic analysis revealed caregivers with higher education levels preferred more grammatical utterances. These findings emphasize the importance of understanding caregiver perspectives in caregiver-mediated intervention approaches.
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Title: THE ROLE OF PREZYGOTIC SELF-COMPATIBILITY IN FACILITATING INTERSPECIFIC COMPATIBILITY IN SOLANUM SECTION PETOTA
Creator: Behling, William
Date: 2022
Collection: Electronic Theses & Dissertations
Description: Domesticated potato (Solanum tuberosum L.) is the fourth most important food crop worldwide and critical food security crop. The high significance of this crop coupled with high production losses place potato at a high priority for genetic improvement, especially in the face of climate change. Potato breeding is undergoing a seismic shift from a tetraploid outcrossing crop towards a F1 hybrid system using diploid inbred lines as parents. This transformation has also changed how potato...
Show moreDomesticated potato (Solanum tuberosum L.) is the fourth most important food crop worldwide and critical food security crop. The high significance of this crop coupled with high production losses place potato at a high priority for genetic improvement, especially in the face of climate change. Potato breeding is undergoing a seismic shift from a tetraploid outcrossing crop towards a F1 hybrid system using diploid inbred lines as parents. This transformation has also changed how potato breeders access important traits from wild species. Pre- and post-zygotic barriers, such as self-incompatibility (SI) and endosperm failure, inhibit and complicate the use of the more valuable wild species in conventional and diploid breeding schemes. Traditionally plant breeders have employed the SC x SI rule, using self-compatibility (SC) to increase the success of interspecific crosses. In order to evaluate the potential of several different SC factors in facilitating broad interspecific compatibility: Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 knockout (KO) lines of S-RNase, HT-B and HT-B + S-RNase in S. tuberosum were evaluated along with the self-compatible species S. verrucosum, and S-locus inhibitor gene (Sli) from S. chacoense. Findings confirm the central role of S-RNase in interspecific pollination barriers and the inconsequential role of HT-B alone. Sli had no effect on interspecific barriers despite its prominent role as a source of SC. Finally, S. verrucosum styles displayed an absence of prezygotic barriers. Further analysis of the role of HT-A, as well as other mechanisms that potentially underlie the SC phenotype in S. verrucosum will further clarify the role of specific genetic factors that regulate interspecific compatibility in Solanum section Petota
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Title: OPPORTUNITIES AND CHALLENGES OF INTEGRATED LARGE SCALE PFAS MODELING
Creator: Raschke, Anna
Date: 2022
Collection: Electronic Theses & Dissertations
Description: Perfluoroalkyl substances (PFAS) have been observed around the world in air, water, and soil. Recent research and monitoring studies have alluded to the widespread presence of PFAS, but most observe the impact of PFAS as a snapshot in time and space. In an effort to better understand PFAS fate and transport in the environment, computational models have been developed. For this study, we synthesized the model applications of PFAS fate and transport via water medium through surface water,...
Show morePerfluoroalkyl substances (PFAS) have been observed around the world in air, water, and soil. Recent research and monitoring studies have alluded to the widespread presence of PFAS, but most observe the impact of PFAS as a snapshot in time and space. In an effort to better understand PFAS fate and transport in the environment, computational models have been developed. For this study, we synthesized the model applications of PFAS fate and transport via water medium through surface water, vadose zone, groundwater, streamflow, as well as their uptake and accumulation in plants and aquatic organisms. In addition, the system under this study is permeable to incoming (sources) and outgoing (sinks) PFAS compounds. Ultimately, knowledge gaps in modeling PFAS for each subsystem (e.g., surface water) area were identified. From there, a case study was performed to highlight the shortcomings of widely used models for PFAS fate and transport within a large and complex watershed. With a large number of PFAS using industries, Michigan is at the forefront of PFAS sampling. Therefore, the study area chosen was the Huron River watershed, a highly PFAS impacted watershed in Southeastern Michigan. The results showed the importance of organized monitoring studies and model improvements to better understand PFAS fate and transport in a large watershed.
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Title: Improving the Efficiency of Residential Buildings in Rural Alaska : An Analysis of Existing Infrastructure and Its Importance in Creating Energy-Efficient Homes
Creator: Milan, Maria
Date: 2022
Collection: Electronic Theses & Dissertations
Description: Rural communities in Alaska, many of which have a high Alaska Native population, currently face significant housing challenges. In a climate that can become extremely cold, houses are generally lacking in energy efficiency. Residents pay high rates for both oil and electricity to heat and operate their homes. Their homes are much smaller and with higher occupancy in comparison to typical U.S. homes. In addition, as the winter season brings sub-zero temperatures, windows remain shut with...
Show moreRural communities in Alaska, many of which have a high Alaska Native population, currently face significant housing challenges. In a climate that can become extremely cold, houses are generally lacking in energy efficiency. Residents pay high rates for both oil and electricity to heat and operate their homes. Their homes are much smaller and with higher occupancy in comparison to typical U.S. homes. In addition, as the winter season brings sub-zero temperatures, windows remain shut with limited mechanical or natural ventilation, also creating indoor air quality concerns. To date there has been limited studies of rural Alaskan communities’ housing, to better understand and quantify such housing challenges. This research focuses on a detailed study of the rural Alaskan community of Unalakleet. Located on the western shore of Alaska, this coastal community of approximately 765 people faces many housing challenges, similar to other rural Alaskan communities. Through collaboration with the housing authority in Unalakleet, this research conducted home energy assessments and resident interviews in the summer of 2021, including 27 energy assessments and 22 interviews. The purpose of the energy assessments was to collect information on typical building features and data, as well as to identify housing challenges. The interviews were generally completed for homes that also had an energy assessment and were used to better understand residents’ perspectives on energy and housing challenges beyond what was observed in the assessments. Blower door tests suggest that homes are usually small and tight, with leakiness around areas like the windows, where mold frequently was observed. Short-term indoor air quality monitoring suggests that some homes, especially those less than 46 m2 , had high CO2 concentrations, relative to others. Some homes had significant mold growth, and others had many areas of damage. Still, interviews with community members suggest that they were grateful for their housing and the ability to live in Unalakleet. The overall purpose of this research is to provide evidence to quantify the typical housing characteristics presence in rural Alaskan communities, as well as to provide results that motivate and support opportunities for new, more efficient housing. The introduction discusses major housing challenges, from high energy bills to the history of inefficient infrastructure in rural Alaska. Physical characteristics of assessed homes, followed by indoor air quality and air flow, are discussed in Chapters 2 and 3, respectively. Finally, Chapter 4 uses data collected from the 27 housing assessments to create a building energy model, where energy usage in existing housing is modeled to represent a ‘typical’ rural Alaskan home. The results of this model show where the largest improvements in efficiency can be made, especially in adding higher R-value insulation. The conclusion provides a brief overview of research contribution, limitations, and future work.
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Title: PRESCRIPTION OPIOIDS, OPIOID USE DISORDERS, AND OPIOID DEATHS ACROSS 21 OECD NATIONS
Creator: Siddiqui, Zehra Jabeen
Date: 2022
Collection: Electronic Theses & Dissertations
Description: Since the 1990s, the United States has seen increased prescription opioid consumption (POC) and an increase in the prevalence of opioid use disorders (OUD) and the opioid disorder death (ODD) rate. This paper examines three factors that may have fueled the opioid crisis: increased supply of prescription opioids, increased demand for opioids because of pain and economic related despair. A regression analysis of twenty-one OECD nations was used to examine the determinants of and the...
Show moreSince the 1990s, the United States has seen increased prescription opioid consumption (POC) and an increase in the prevalence of opioid use disorders (OUD) and the opioid disorder death (ODD) rate. This paper examines three factors that may have fueled the opioid crisis: increased supply of prescription opioids, increased demand for opioids because of pain and economic related despair. A regression analysis of twenty-one OECD nations was used to examine the determinants of and the relationships between POC, OUDs, and ODDs. Over 1991-2019, consumption did not have a significant effect on OUDs or ODDs, but over 2003-2019 it had a positive significant effect on both. These results held if the US was removed from the sample, supporting some supply side effect across the panels after 2003. Furthermore, OUDs have a large, positive and significant effect on ODDs, indicating two possible pathways direct (straight from consumption) and indirect (through an effect on opioid use disorders) that POC relates to increased ODDs. One model estimates that 39% of the increase in US opioid fatalities is attributable to increased POC between 2003-2010.
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Title: WE DON’T NEED TO KNOW WHAT WE SEE : MODEST MEDIATION OF BISTABLE PERCEPTION BY KNOWLEDGE
Creator: Zhang, Bobicheng
Date: 2022
Collection: Electronic Theses & Dissertations
Description: When faced with ambiguous visual input, an observer may have various perceptual interpre- tations even when the input remains unchanged. Indeed, such ambiguous displays can cause the observer to experience distinct perceptual interpretations in turn, unpredictably switching between them over time. Theories of such so-called multistable perception broadly fall into two categories: top-down theories that hold that perception in these cases depends strongly on higher-level cogni- tive factors...
Show moreWhen faced with ambiguous visual input, an observer may have various perceptual interpre- tations even when the input remains unchanged. Indeed, such ambiguous displays can cause the observer to experience distinct perceptual interpretations in turn, unpredictably switching between them over time. Theories of such so-called multistable perception broadly fall into two categories: top-down theories that hold that perception in these cases depends strongly on higher-level cogni- tive factors such as knowledge, and bottom-up theories which suggests more vital involvement of aspects of lower-order information processing such as local adaptation in the visual system. We evaluated whether the occurrence of perceptual reversals in the face of ambiguous input is related to the observer’s knowledge that the input is, indeed, ambiguous. We used an ambiguous animation that was designed such that subjects could report perceptual reversals without realizing the ambigu- ity. Subjects observed the animation, reported their perception, and filled out a questionnaire that assessed their knowledge of the animation’s ambiguity. We found that informed subjects reported slightly more perceptual switches than the other subjects, but that this between-group difference was very small compared to the lack of variability within each group between subjects who were aware of the ambiguity and those who were not. These findings suggests that knowledge of ambiguity can influence perception of ambiguous stimuli, but that this influence is relatively minor. This discrepancy between current findings and past work is discussed.
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Title: Evaluation of potential therapeutics for non-small cell lung cancer using 3D tumor spheroid models
Creator: Stoub, Hayden Eric
Date: 2022
Collection: Electronic Theses & Dissertations
Description: The development of novel and improved therapies for cancers requires robust means of investigation that both effectively recapitulate tumor biology while also utilizing resources efficiently. 3D tissue culture methods, namely spheroids and organoids, have emerged as an effective bridge between conventional in vitro methods and in vivo animal models in cancer research. This thesis provides background into lung cancer and the current landscape of 3D in vitro models of cancer, as well as...
Show moreThe development of novel and improved therapies for cancers requires robust means of investigation that both effectively recapitulate tumor biology while also utilizing resources efficiently. 3D tissue culture methods, namely spheroids and organoids, have emerged as an effective bridge between conventional in vitro methods and in vivo animal models in cancer research. This thesis provides background into lung cancer and the current landscape of 3D in vitro models of cancer, as well as detailing two studies in which multicellular tumor spheroids were used to investigate the therapeutic potential of candidate compounds. The first study uses an NCI-H358 multicellular tumor spheroid to investigate three compounds: Apigenin, a plant derived flavonoid; MSU42011, a novel retinoid x receptor (RXR) agonist; and CEP-1347, a mixed lineage kinase (MLK) inhibitor. It was found that Apigenin alone decreased the viability of spheroids, and that the combination of Apigenin and CEP-1347 synergistically decreased viability and increased cell death within the spheroids.The second study uses a murine lung adenocarcinoma tumor spheroid model to investigate the effects of MSU-71, a novel inhibitor of the nuclear factor erythroid 2-related factor (NRF2) pathway, on macrophage-induced invasion and migration. This compound blocked both migration in conventional experiments along with spheroid invasion, indicating its potential efficacy in preventing LUAD progression. This study also adapted the spheroid invasion model for use in multiple human LUAD cell lines and THP-1 conditioned medium. The findings presented herein demonstrate the versatility and value of 3D tumor spheroid models in the cancer drug development field.
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Title: BLAME JUDGEMENTS FOR PAST ACTIONS OF GROUPS
Creator: Vallabha, Shree
Date: 2022
Collection: Electronic Theses & Dissertations
Description: Historically, groups have committed innumerable atrocities, including genocide and slavery. I tested if people blame current groups for the actions of their past members and what underlies this blame. Current models of blame overlook the dimension of time and therefore have difficulty explaining this phenomenon. I hypothesized that perceiving higher (a) connectedness between past and present perpetrator groups, (b) unfulfilled obligations of perpetrator groups, (c) continued privilege of...
Show moreHistorically, groups have committed innumerable atrocities, including genocide and slavery. I tested if people blame current groups for the actions of their past members and what underlies this blame. Current models of blame overlook the dimension of time and therefore have difficulty explaining this phenomenon. I hypothesized that perceiving higher (a) connectedness between past and present perpetrator groups, (b) unfulfilled obligations of perpetrator groups, (c) continued privilege of perpetrator groups, and (d) continued harm of victim groups would facilitate higher blame judgements against current groups for the past. In a survey (N=518) using real events, I find results consistent with all the four hypotheses and some preliminary evidence for group differences in these relationships. I find that factors that link the present group to the past explain why groups are blamed for their past actions. These findings bring to light the deficiencies in existing theories of blame – calling into question its assumptions and incomplete criterion of judgement.
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Title: Memory-efficient emulation of physical tabular data using quadtree decomposition
Creator: Carlson, Jared
Date: 2022
Collection: Electronic Theses & Dissertations
Description: Computationally expensive functions are sometimes replaced in simulations with an emulator that approximates the true function (e.g., equations of state, wavelength-dependent opacity, or composition-dependent materials properties). For functions that have a constrained domain of interest, this can be done by discretizing the domain and performing a local interpolation on the tabulated function values of each local domain. For these so-called tabular data methods, the method of discretizing...
Show moreComputationally expensive functions are sometimes replaced in simulations with an emulator that approximates the true function (e.g., equations of state, wavelength-dependent opacity, or composition-dependent materials properties). For functions that have a constrained domain of interest, this can be done by discretizing the domain and performing a local interpolation on the tabulated function values of each local domain. For these so-called tabular data methods, the method of discretizing the domain and mapping the input space to each subdomain can drastically influence the memory and computational costs of the emulator. This is especially true for functions that vary drastically in different regions. We present a method for domain discretization and mapping that utilizes quadtrees, which results in significant reductions in the size of the emulator with minimal increases to computational costs or loss of global accuracy. We apply our method to the electron-positron Helmholtz free energy equation of state and show over an order of magnitude reduction in memory costs for reasonable levels of numerical accuracy.
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Title: Analysis of Positional Bias Within Multiple Stimulus Without Replacement Preference Assessments
Creator: Miranda, David Ray Gutierrez
Date: 2021
Collection: Electronic Theses & Dissertations
Description: Positional bias is a pattern of responding to a specific location that can be influenced by response effort and/or prior learning history. Within the contexts of preference assessments, positional bias create additional variables that make ascertaining true preferences within a preference assessment more difficult. Prior research on positional bias within preference assessments have focused primarily on its use in paired stimulus assessments due to the complex nature of the multiple-stimulus...
Show morePositional bias is a pattern of responding to a specific location that can be influenced by response effort and/or prior learning history. Within the contexts of preference assessments, positional bias create additional variables that make ascertaining true preferences within a preference assessment more difficult. Prior research on positional bias within preference assessments have focused primarily on its use in paired stimulus assessments due to the complex nature of the multiple-stimulus without replacement preference assessment. The present study is a secondary analysis that utilized four different methods to measure side bias and center bias for 19 young children with autism spectrum disorders. Results indicate that participants had varying degrees of biased responding but collectively engaged in little biased responding. Present study includes discussion of general patterns of responding, an analysis of the four methodologies, and general recommendations for the application of these methodologies.
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Title: GENERATION OF HT-B AND HT-B PLUS S-RNASE KNOCKOUT LINES TO UNDERSTAND SELF-COMPATIBILITY IN DIPLOID POTATO
Creator: Lee, Sarah
Date: 2021
Collection: Electronic Theses & Dissertations
Description: Domesticated potato (Solanum tuberosum L.) is the world’s third most important food crop and is a food security crop according to the Food and Agriculture Organization of the United Nations. Currently, commercial potatoes are autotetraploid and mainly produced via asexual clonal propagation. The autotetraploid nature of most cultivated potatoes in combination with acute inbreeding depression when self-fertilized over multiple generations cause challenges in making advances with traditional...
Show moreDomesticated potato (Solanum tuberosum L.) is the world’s third most important food crop and is a food security crop according to the Food and Agriculture Organization of the United Nations. Currently, commercial potatoes are autotetraploid and mainly produced via asexual clonal propagation. The autotetraploid nature of most cultivated potatoes in combination with acute inbreeding depression when self-fertilized over multiple generations cause challenges in making advances with traditional breeding schemes. The benefits of moving potato to a diploid breeding model include a simplified breeding scheme, easier fixation of desirable alleles, and generation of inbred lines that may be used to generate F1 hybrids with heterotic potential. A major hinderance to self-compatibility originates from the gametophytic self-incompatibility (GSI) system in which the S-RNase and HT-B genes play a critical role. Utilizing CRISPR-Cas9 gene editing, HT-B and HT-B + S-RNase knockout (KO) lines were produced. HT-B KOs produced parthenocarpic fruit but remained self-incompatible. However, the S-RNase and HT-B double KOs were self-compatible. Self-compatibility was measured quantitatively using fruit set, fruit weight, and seed count. Fruit set varied across both self-incompatible and self-compatible lines, with no clear trend in statistical significance. Double KO lines consistently displayed higher fruit weight than incompatible lines. Seed count served as the best measure of self-compatibility, with S-RNase and HT-B double KO lines producing up to three times mean seed per fruit when compared to S-RNase only KOs from prior studies. The lines with the highest levels of self-compatibility will serve as useful additions in advancing potato breeding to the diploid level.
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Title: EXPLORING THE IMPACT OF BIOGAS QUANTITY AND QUALITY IN DIFFERENT DIGESTER TYPES WITH VARIATIONS IN TEMPERATURE
Creator: Barrios Arosemena, Maria Ines
Date: 2021
Collection: Electronic Theses & Dissertations
Description: The energy sector in the U.S. has been pushing for policies such as the Renewable Portfolio Standard (RPS) to mitigate the impacts of GHG emissions. Biogas from anaerobic digesters is a viable form of renewable energy, due to its CH4 composition, it can be used as a replacement for power and heat generation or upgraded and sold as biomethane. This study analyzed the effects of temperature in biogas quality and quantity of dairy cow manure in order to compare two main systems, a CSTR and a...
Show moreThe energy sector in the U.S. has been pushing for policies such as the Renewable Portfolio Standard (RPS) to mitigate the impacts of GHG emissions. Biogas from anaerobic digesters is a viable form of renewable energy, due to its CH4 composition, it can be used as a replacement for power and heat generation or upgraded and sold as biomethane. This study analyzed the effects of temperature in biogas quality and quantity of dairy cow manure in order to compare two main systems, a CSTR and a covered lagoon. A biochemical methane potential (BMP) test was performed to determine material biodegradability of dairy cow manure with respect to temperature. The results show that all samples are anaerobically biodegradable with samples yielding 86, 168, 440, 475 and 448 L biogas per kg initial VS for 15°C, non-mixed; 20°C, non-mixed; 30°C, non-mixed; 39°C, non-mixed; and 39°C, mixed, respectively. The BMP results demonstrated so significant difference between 30°C, non-mixed; 39°C, non-mixed; and 39°C, mixed, respectively. In addition, the effects of psychrophilic, unregulated, and mesophilic conditions were tested in small scale lab pilot digesters. Results show that mesophilic condition yielded the highest cumulative biogas production, while the psychrophilic and unregulated conditions presented higher methane yield. A life cycle analysis was performed to compare two popular anaerobic digestion systems, a CTSR and a covered lagoon, versus current manure management systems for dairy cow manure. The LCA revealed that both systems have less environmental burdens when compared to current waste management systems and a CSTR has less environmental burdens than a covered lagoon.
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Title: SOLIDARITY & RESISTANCE : WOMEN’S PERSPECTIVES ON EMPOWERMENT THROUGH SELF HELP GROUPS
Creator: Mishra, Manasi
Date: 2021
Collection: Electronic Theses & Dissertations
Description: Development interventions mostly aim to ‘empower’ rural women by increasing their income and livelihood but rarely consider the voices of their beneficiaries. I study the meanings and experiences of empowerment or nari shakti as understood and described by rural women who lead self-help groups (SHGs) engaged in savings, micro-credit and income generation. I conducted the research in the central Himalayan region of rural India in Uttarakhand State using in-depth interviews with 11 SHG leaders....
Show moreDevelopment interventions mostly aim to ‘empower’ rural women by increasing their income and livelihood but rarely consider the voices of their beneficiaries. I study the meanings and experiences of empowerment or nari shakti as understood and described by rural women who lead self-help groups (SHGs) engaged in savings, micro-credit and income generation. I conducted the research in the central Himalayan region of rural India in Uttarakhand State using in-depth interviews with 11 SHG leaders. The findings indicate that the participants experience empowerment as solidarity and collective power that manifest in the form of self-reliance, courage, and independence. The participants associate their self-reliance with courage and inner strength which they have built as a means to survive the pain and struggle. Their stories of nari shakti are also stories of pain and struggle.This research implies the need to examine perspectives of feminists on empowerment related interventions. Further research is needed on how micro-credit SHGs help build collective action that can challenge patriarchal power structures and bring about social change. Such research can help provide solutions on how non-profits can utilize feminist strategies of consciousness raising while still meeting donor expectations for the SHG programs.
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Title: PRECISION DIAGNOSTICS AND INNOVATIONS FOR PLANT BREEDING RESEARCH
Creator: Hugghis, Eli
Date: 2021
Collection: Electronic Theses & Dissertations
Description: Major technological advances are necessary to reach the goal of feeding our world’s growing population. To do this, there is an increasing demand within the agricultural field for rapid diagnostic tools to improve the efficiency of current methods in plant disease and DNA identification. The use of gold nanoparticles has emerged as a promising technology for a range of applications from smart agrochemical delivery systems to pathogen detection. In addition to this, advances in image...
Show moreMajor technological advances are necessary to reach the goal of feeding our world’s growing population. To do this, there is an increasing demand within the agricultural field for rapid diagnostic tools to improve the efficiency of current methods in plant disease and DNA identification. The use of gold nanoparticles has emerged as a promising technology for a range of applications from smart agrochemical delivery systems to pathogen detection. In addition to this, advances in image classification analyses have allowed machine learning approaches to become more accessible to the agricultural field. Here we present the use of gold nanoparticles (AuNPs) for the detection of transgenic gene sequences in maize and the use of machine learning algorithms for the identification and classification of Fusarium spp. infected wheat seed. AuNPs show promise in their ability to diagnose the presence of transgenic insertions in DNA samples within 10 minutes through colorimetric response. Image-based analysis with the utilization of logistic regression, support vector machines, and k-nearest neighbors were able to accurately identify and differentiate healthy and diseased wheat kernels within the testing set at an accuracy of 95-98.8%. These technologies act as rapid tools to be used by plant breeders and pathologists to improve their ability to make selection decisions efficiently and objectively.
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Title: TEACHING STAFF TO IMPLEMENT MAND TRAINING WITH CHILDREN WITH ASD THROUGH TELEHEALTH
Creator: Rosalik, Shelby R.
Date: 2021
Collection: Electronic Theses & Dissertations
Description: Telehealth services have increased substantially in the field of applied behavior analysis (ABA) since the start of the COVID-19 pandemic, though little research exists to empirically evaluate the efficacy of direct ABA telehealth treatment or more specifically, how behavior technicians can be trained to implement such treatment. The present investigation utilized a nonconcurrent multiple baseline design across participants to evaluate the use of an online behavioral skills training (BST)...
Show moreTelehealth services have increased substantially in the field of applied behavior analysis (ABA) since the start of the COVID-19 pandemic, though little research exists to empirically evaluate the efficacy of direct ABA telehealth treatment or more specifically, how behavior technicians can be trained to implement such treatment. The present investigation utilized a nonconcurrent multiple baseline design across participants to evaluate the use of an online behavioral skills training (BST) approach to teach behavior technicians to implement 20-min mand training sessions via telehealth with children diagnosed with autism spectrum disorder (ASD). The training phase of the study consisted of both role-play with feedback as well as feedback during sessions with the child participant. Results showed increases in behavior technician’s percentage of accurate implementation and rate of fully correct trials implemented following the training. Child participants also showed increased rates of independent mands following the BST implementation. Thus, BST may be an effective approach to teach behavior technicians to deliver mand training via telehealth.
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Title: CHARACTERIZING THE SOCIAL GAP IN UTILITY-SCALE SOLAR ENERGY
Creator: Crawford, Jessica Alex
Date: 2021
Collection: Electronic Theses & Dissertations
Description: Many consider utility-scale photovoltaic solar power to be an essential component of decarbonizing the United States power sector and mitigating climate change. This technology is well accepted by the public in general surveys, yet often faces local resistance during project siting. This phenomenon is known as the “social gap.” Using social gap theory from the wind energy literature as a foundation, this study examines the causes of and offers recommendations for addressing the solar social...
Show moreMany consider utility-scale photovoltaic solar power to be an essential component of decarbonizing the United States power sector and mitigating climate change. This technology is well accepted by the public in general surveys, yet often faces local resistance during project siting. This phenomenon is known as the “social gap.” Using social gap theory from the wind energy literature as a foundation, this study examines the causes of and offers recommendations for addressing the solar social gap in Michigan. The study relied on 33 semi-structured interviews with citizens, government officials, and developers across four Michigan communities, each facing a prospective utility-scale solar project. Through thematic analysis, I show that the solar social gap can be attributed to both a vocal minority that dominated community sentiment and project proposals that failed to meet the community’s standards for acceptable development. The gap was exacerbated by the presence of organized opposition groups as well as decision-makers relying on ineffective public processes to engage citizens. This research makes it clear that government officials and developers need to adopt practices that enhance community representation, process transparency, and decision-influence. Though decision-making strategies are not the only factor that affects community acceptance, implementing improved procedures could help close the solar social gap.
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