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(1 - 8 of 8)
- Title
- Cytology and seed set studies in the pansy, Viola tricolor Hortensis L
- Creator
- Emino, Everett Raymond, 1942-
- Date
- 1967
- Collection
- Electronic Theses & Dissertations
- Title
- From skin to neurons : examining variations in reprogramming efficiency
- Creator
- Keaton, Sarah A.
- Date
- 2013
- Collection
- Electronic Theses & Dissertations
- Description
-
Cellular reprogramming is a newly emerging field with promising clinical applications. The ability to generate non-dividing crucial cell types from rapidly proliferating cell types, the potential to heal diseased people who do not have many treatment options, being able to bypass immune rejection, and avoid invasive surgery has captured the media's attention. However, there have been disparities in the efficiency of reprogramming and these needs to be addressed before cellular reprogramming...
Show moreCellular reprogramming is a newly emerging field with promising clinical applications. The ability to generate non-dividing crucial cell types from rapidly proliferating cell types, the potential to heal diseased people who do not have many treatment options, being able to bypass immune rejection, and avoid invasive surgery has captured the media's attention. However, there have been disparities in the efficiency of reprogramming and these needs to be addressed before cellular reprogramming can be applicable in a clinical setting. To better understand the variations of cellular reprogramming, human and mouse fibroblasts were converted into induced neural cells in an attempt to unveil the impact of disease state, tissue origin and genetics. The experimental results indicate reprogramming efficiency was reproducible within a primary fibroblast line however there was a dramatic difference between lines even from an isogenic source. Testing a larger number of fibroblast lines, even lines with the identical genetic backgrounds and tissue origins, is likely the most direct means of improving reprogramming efficiency and enabling this procedure to be available for therapeutic use.
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- Title
- Impact of ploidy on morphological variation in Arizona phlox, Phlox amabilis (Polemoniaceae)
- Creator
- Chansler, Matthew Thomas
- Date
- 2015
- Collection
- Electronic Theses & Dissertations
- Description
-
Polyploidy is an important factor in the evolution and ecology of flowering plants. A better understanding of the kind and degree of morphological differentiation among ploidy levels within a species can help explain further how polyploidy affects biodiversity. How widespread is the impact of ploidy across the phenotype of a species? Which aspects of morphology vary, and do they vary consistently? How does ploidy relate to overall morphological diversity? Do ploidy levels have detectable...
Show morePolyploidy is an important factor in the evolution and ecology of flowering plants. A better understanding of the kind and degree of morphological differentiation among ploidy levels within a species can help explain further how polyploidy affects biodiversity. How widespread is the impact of ploidy across the phenotype of a species? Which aspects of morphology vary, and do they vary consistently? How does ploidy relate to overall morphological diversity? Do ploidy levels have detectable phenotypic profiles? Finally, are there morphological differences between populations, potentially due to environment or evolutionary changes since formation apparent in natural populations? I assessed morphological variation within Arizona phlox, Phlox amabilis. This species of conservation concern is endemic to Arizona, and prior work has detected diploid, tetraploid, and hexaploid populations. I sampled 11 populations of P. amabilis, covering a large portion of the species’ range. A wide array of morphological features, including characters that described cell size, overall habit, leaf dimensions, and floral dimensions, were measured for up to 25 plants at each population. Significant differences were detected in 15 out of 27 characters using mixed GLM. A large amount of overall morphological variation is explained by the differences between ploidy levels, and each ploidy level can be described by a specific multivariate phenotype with 95% accuracy. Finally, although overall structuring was influenced by ploidy, differences among populations still contributed a high degree of variation in the morphospace of Phlox amabilis. This morphological assessment will be integrated with ecological and genetic data to build a more complete understanding of the interplay between these factors and ploidy in Phlox amabilis.
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- Title
- Jasmonate regulation of defense responses in tomato (Lycopersicon esculentum)
- Creator
- Liu, Guanghui
- Date
- 2004
- Collection
- Electronic Theses & Dissertations
- Title
- On the isolation of basal bodies from Tetrahymena pyriformis
- Creator
- Linney, Elwood A.
- Date
- 1967
- Collection
- Electronic Theses & Dissertations
- Title
- Rat colon epithelial cells : isolation, cultivation, and benzo(a)pyrene metabolism
- Creator
- Skrypec, Daniel J.
- Date
- 1981
- Collection
- Electronic Theses & Dissertations
- Title
- The cytologic responses of normal beagle dogs utilizing the skin window technic
- Creator
- Drees, David T.
- Date
- 1966
- Collection
- Electronic Theses & Dissertations
- Title
- Therapeutically targeting autophagy in non-small cell lung cancer
- Creator
- Yco, Lisette Pangilinan
- Date
- 2019
- Collection
- Electronic Theses & Dissertations
- Description
-
Autophagy is a conserved catabolic pathway which sequesters intracellular components in lysosomes to recycle macromolecules for cell maintenance. The role of autophagy in tumor cells is dynamic and depends on many factors including tumor types, tumor stages, and activity of several tumor suppressors and oncogenes. In this thesis, I wanted to improve our understanding of the unique relationship of autophagy with tumor suppressor p53 and oncogenic KRAS in cancer cells, particularly in NSCLC....
Show moreAutophagy is a conserved catabolic pathway which sequesters intracellular components in lysosomes to recycle macromolecules for cell maintenance. The role of autophagy in tumor cells is dynamic and depends on many factors including tumor types, tumor stages, and activity of several tumor suppressors and oncogenes. In this thesis, I wanted to improve our understanding of the unique relationship of autophagy with tumor suppressor p53 and oncogenic KRAS in cancer cells, particularly in NSCLC. First, I demonstrated that stabilized nuclear wild-type p53 through HDM2 inhibition with MK-8242 or nutlin-3a could induce autophagy in tumor cells through transactivation of several autophagy-related genes (DRAM, FOXO3A, SESN2, and MRCKα) and autophagy core genes (ATG4A and ULK1). In addition, I found that inhibiting of KRAS G12C signaling and suppressing mTORC1 activity by selective KRAS G12C inhibitor, ARS-853, could drive autophagy response in KRAS G12C NSCLC cell lines. Since autophagy could also promote survival under stress induced by several anticancer agents, I designed a combination study using newly reported selective ULK1 inhibitor, ULK-101, with ARS-853 in KRAS mutant NSCLC. Autophagy inhibition with ULK-101 dramatically enhanced the ability of selective KRAS G12C inhibitor to impair the viability of KRAS G12C NSCLC. Together, my study provided evidence that autophagy serves as a survival pathway in tumor cells and that future assessment of small molecule that target autophagy core proteins may be potential cancer therapeutic option in p53 wild-type and KRAS G12C NSCLC.
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