PRECISION DIAGNOSTICS AND INNOVATIONS FOR PLANT BREEDING RESEARCH

Major technological advances are necessary to reach the goal of feeding our world’s growing population. To do this, there is an increasing demand within the agricultural field for rapid diagnostic tools to improve the efficiency of current methods in plant disease and DNA identification. The use of gold nanoparticles has emerged as a promising technology for a range of applications from smart agrochemical delivery systems to pathogen detection. In addition to this, advances in image classification analyses have allowed machine learning approaches to become more accessible to the agricultural field. Here we present the use of gold nanoparticles (AuNPs) for the detection of transgenic gene sequences in maize and the use of machine learning algorithms for the identification and classification of Fusarium spp. infected wheat seed. AuNPs show promise in their ability to diagnose the presence of transgenic insertions in DNA samples within 10 minutes through colorimetric response. Image-based analysis with the utilization of logistic regression, support vector machines, and k-nearest neighbors were able to accurately identify and differentiate healthy and diseased wheat kernels within the testing set at an accuracy of 95-98.8%. These technologies act as rapid tools to be used by plant breeders and pathologists to improve their ability to make selection decisions efficiently and objectively.